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Comparative proteomics analysis of whey fractions from Korean mother's milk with different gender babies in the northeast China 中国东北朝鲜族不同性别婴儿母乳乳清组分的蛋白质组学比较分析
IF 4.4 2区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-01-22 DOI: 10.1111/1471-0307.13053
Boxue Xia, Yingcong Lu, Dan Li, Jianjun Cheng, Shilong Jiang, Mingruo Guo, Cuina Wang
Infant's gender may have influences on composition of breastmilk. This study investigated differences in whey fractions from milk of mothers (n = 12, 3 biological repeats) with male or female babies using a 4D label-free proteomics. Altogether, 1572 proteins were identified and 42 of them were differentially expressed. Majority of the different proteins are located in extracellular position and possessed domain of immunoglobulin V-set. The screened proteins were observed to contribute greatly to functions of cellular process, binding, and cell part; and pathways of immune system and immune disease. The findings may afford beneficial knowledge for understanding differences in breastmilk composition.
婴儿的性别可能会影响母乳的成分。本研究利用 4D 无标记蛋白质组学研究了男婴或女婴母亲(n = 12,3 个生物学重复)乳汁中乳清组分的差异。共鉴定出 1572 种蛋白质,其中 42 种蛋白质的表达存在差异。大多数不同的蛋白质都位于细胞外位置,并具有免疫球蛋白 V 组的结构域。据观察,筛选出的蛋白质对细胞过程、结合和细胞部分的功能,以及免疫系统和免疫疾病的途径有很大贡献。这些发现可为了解母乳成分的差异提供有益的知识。
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引用次数: 0
The effect of soluble soybean polysaccharide addition to permeate concentrate on lactose crystallisation, growth and recovery during lactose manufacturing 在乳糖生产过程中,向渗透浓缩物中添加可溶性大豆多糖对乳糖结晶、生长和回收的影响
IF 4.4 2区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-01-19 DOI: 10.1111/1471-0307.13050
Venkateswarlu Sunkesula, Prafulla Salunke, Lloyd E Metzger
Lactose is manufactured from permeates of cheese whey, milk protein concentrate and a mix of both using a crystallisation process. Several factors, such as solute supersaturation, cooling rate and impurities, influence lactose crystallisation. This study focussed on the effect of soluble soybean polysaccharides (SSPS) on crystallisation during lactose manufacture from concentrated permeate solutions by evaluating lactose crystal growth and recovery with and without SSPS addition. Adding 0.1% (w/w) SSPS resulted in a significant difference (P > 0.05) in lactose crystal size and yield. The results suggest that using SSPS during lactose manufacturing from permeate concentrates could influence lactose crystallisation and recovery.
乳糖是利用结晶工艺从奶酪乳清、浓缩牛奶蛋白和两者的混合渗透物中制造出来的。溶质过饱和度、冷却速度和杂质等多种因素都会影响乳糖的结晶。本研究通过评估添加和不添加可溶性大豆多糖(SSPS)的乳糖晶体生长和回收率,重点研究了可溶性大豆多糖(SSPS)对从浓缩渗透液生产乳糖过程中结晶的影响。添加 0.1%(重量比)的 SSPS 会导致乳糖晶体大小和产量的显著差异(P > 0.05)。结果表明,在利用渗透浓缩物生产乳糖的过程中使用 SSPS 可能会影响乳糖的结晶和回收。
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引用次数: 0
Triglyceride and fatty acid composition of bovine colostrum and transition milk in pasture-based dairy cows supplemented prepartum with inorganic selenium, organic selenium or rumen-protected choline 产前补充无机硒、有机硒或瘤胃保护胆碱的牧场奶牛初乳和过渡乳的甘油三酯和脂肪酸组成
IF 4.4 2区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-01-17 DOI: 10.1111/1471-0307.13051
Fionnuala McDermott, Emer Kennedy, Gaetan Drouin, Lorraine Brennan, Tom F. O'Callaghan, Michael Egan, Sean A. Hogan
The objectives of this study were to determine the effects of prepartum supplementation with inorganic selenium (INORG), organic selenium (ORG) or a rumen-protected choline, on the triglyceride (TAG) and total fatty acid (TFA) profiles of bovine colostrum and transition milk. Fifty-seven Holstein Friesian (HF) and HF × Jersey cows were supplemented daily from 49 ± 12.9 days prior to calving until day of calving. Milk samples were analysed for TAG and TFA composition using GC-FID. Prepartum supplementation did not have a significant effect on the milk lipid profiles. This study quantified colostrum lipids not previously reported in the literature.
本研究旨在确定产前补充无机硒(INORG)、有机硒(ORG)或瘤胃保护胆碱对牛初乳和过渡乳甘油三酯(TAG)和总脂肪酸(TFA)含量的影响。57头荷斯坦弗里斯兰(HF)奶牛和HF×娟珊(Jersey)奶牛从产犊前49±12.9天开始至产犊当日,每天接受补充营养。使用 GC-FID 分析牛奶样本中的 TAG 和 TFA 成分。产前添加辅食对牛奶脂质没有显著影响。这项研究对初乳脂质进行了定量分析,而此前的文献中并没有相关报道。
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引用次数: 0
Characterisation of extracellular vesicles properties and miRNAs profiles in Brown Swiss milk 褐瑞士牛奶中细胞外囊泡特性和 miRNAs 图谱的表征
IF 4.4 2区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-01-16 DOI: 10.1111/1471-0307.13049
Chunmei Du, Suyu Quan, Jian Ma, Fuquan Yin, Mengsi Xu, Liuxia Lin, Guangxian Zhou, Jiang Wu, Shangquan Gan
The conserved extracellular vesicles (EVs)-miRNAs from milk might potentially influence human gene expression, or even introduce their analogous nucleotide chains into the human food chain. This study aimed to characterise the features of Brown Swiss milk EVs and the EVs-miRNAs profile. Brown Swiss milk EVs exhibited a cup-shaped morphology, with a distribution of (2.3 ± 0.8) E + 11 particles/mL. Sequencing identified a total of 238 miRNAs, which were mainly implicated in immune response and cell signal transduction. The conservation of these milk EVs-miRNAs among species suggested they might be evolutionarily selected to regulate the growth and development of the newborns.
牛奶中的保守细胞外囊泡 (EVs) -miRNAs 有可能影响人类基因的表达,甚至将其类似的核苷酸链引入人类食物链。本研究旨在描述棕瑞牛奶EVs的特征以及EVs-miRNAs的概况。棕色瑞士牛奶的EVs呈杯状形态,分布为(2.3 ± 0.8)E + 11颗粒/毫升。测序共鉴定出 238 个 miRNAs,它们主要与免疫反应和细胞信号转导有关。这些牛奶EVs-miRNAs在物种间的保留表明,它们可能是进化选择出来的,用于调节新生儿的生长发育。
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引用次数: 0
Journal News and Editor's Choice Article for February 2024 2024 年 2 月期刊新闻和编辑推荐文章
IF 4.4 2区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-01-15 DOI: 10.1111/1471-0307.13048
Michael Mullan
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引用次数: 0
Evaluation of low sodium Kačkavalj cheese properties using 3D scanning, scanning electron microscopy and computer vision system 利用三维扫描、扫描电子显微镜和计算机视觉系统评估低钠卡卡瓦利奶酪的特性
IF 4.4 2区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-01-12 DOI: 10.1111/1471-0307.13046
Ana Satric, Igor Tomasevic, Ilija Djekic, Vladimir Pavlovic, Steva Levic, Jelena Miocinovic
Salt reduction in food is one of the main concerns for public health agencies. Performing two-way analysis of variance, the effects of salt reduction and replacement on the pasta filata Kačkavalj cheese properties were studied during 6 months. Computer vision system is a novel method for colour evaluation. There were no differences in colour parameters L*, a* and b* (90.40–92.87, 0.07–0.60 and 11.40–18.53 respectively) and microstructure analysed by scanning electron microscopy between variants during ripening. Three-dimensional scanning revealed that low sodium variants showed lower volume reduction. Salt reduction in Kačkavalj cheese results in high quality product and contributes to consumer health.
减少食品中的盐分是公共卫生机构关注的主要问题之一。通过双向方差分析,研究了 6 个月期间减盐和换盐对意大利面 Filata Kačkavalj 奶酪特性的影响。计算机视觉系统是一种新的颜色评估方法。在成熟过程中,不同品种之间的颜色参数 L*、a* 和 b*(分别为 90.40-92.87、0.07-0.60 和 11.40-18.53)以及扫描电子显微镜分析的微观结构没有差异。三维扫描显示,低钠变种的体积减少较少。减少 Kačkavalj 奶酪中的盐分可获得高质量的产品,并有助于消费者的健康。
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引用次数: 0
The effect of incorporating microencapsulated Lactobacillus casei ATCC 334 on the physicochemical and rheological properties of fortified Greek-style yoghurt 加入微胶囊干酪乳杆菌 ATCC 334 对强化希腊酸奶的理化和流变特性的影响
IF 4.4 2区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-01-09 DOI: 10.1111/1471-0307.13047
Beneranda Murua-Pagola, Lucía Abadía-García, Maria Isabel Vázquez-Aguilar, Silvia L Amaya-Llano, Eduardo Morales-Sánchez
This work evaluated the physicochemical and rheological properties of Greek-style yoghurt supplemented with microencapsulated Lactobacillus casei ATCC 334 cells. The microencapsulation was able to protect the probiotic cells during yoghurt fermentation, and up to 21 days of cold storage, as well as to the exposure under gastrointestinal conditions. Samples added with free cells showed the lowest values of G′ (867 ± 31.76) after 24 h of fermentation and the highest values of G′ and G″ after 14 days of cold storage. The yoghurt added with microencapsulated cells exhibited greater firmness and the lowest adhesiveness values.
这项研究对添加了微胶囊干酪乳杆菌 ATCC 334 细胞的希腊式酸奶的理化和流变特性进行了评估。微胶囊能够在酸奶发酵、长达 21 天的冷藏以及胃肠道暴露条件下保护益生菌细胞。添加了游离细胞的样品在发酵 24 小时后显示出最低的 G′值(867 ± 31.76),而在冷藏 14 天后显示出最高的 G′和 G″值。添加了微胶囊细胞的酸奶显示出更高的硬度和最低的粘附性值。
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引用次数: 0
Whey protein‐derived peptides improve blood profile and enhance muscular endurance in mice 源于乳清蛋白的肽能改善小鼠的血液状况并增强肌肉耐力
IF 4.4 2区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-01-05 DOI: 10.1111/1471-0307.13038
Da-min Jung, Woo‐Jin Ki, Young‐Soon Lim, Myoung‐Soo Nam, Kee K. Kim
Whey protein‐derived peptides (WPP) can exert diverse physiological activities. However, their effects on exercise ability remain largely unknown. Herein, we investigated the effects of WPP on various physiological activities in cells, as well as on the blood profile and exercise ability of mice, aiming to develop bioactive peptides for enhancing muscular endurance. The intake of WPP induced a positive blood profile by lowering total cholesterol and significantly enhanced the muscular endurance of mice in treadmill endurance tests. Collectively, our results suggest the usefulness of WPP as agents for inducing vascular health, contributing to exercise ability by enhancing muscular endurance.
乳清蛋白衍生肽(WPP)可发挥多种生理活性。然而,它们对运动能力的影响在很大程度上仍然未知。在此,我们研究了 WPP 对细胞中各种生理活性以及小鼠血液特征和运动能力的影响,旨在开发用于增强肌肉耐力的生物活性肽。摄入 WPP 可降低总胆固醇,从而改善血液状况,并显著增强小鼠在跑步机耐力测试中的肌肉耐力。总之,我们的研究结果表明,WPP 可作为诱导血管健康的药物,通过增强肌肉耐力来提高运动能力。
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引用次数: 0
Cross-linking duration impacts on Hatay cheeses with microbial transglutaminase enzyme 微生物转谷氨酰胺酶交联时间对哈塔伊奶酪的影响
IF 4.4 2区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-01-04 DOI: 10.1111/1471-0307.13044
Nuray Güzeler, Çağla Özbek, Hüseyin Mert
The study aimed to enhance Hatay cheese texture using microbial transglutaminase enzyme (mTG). Cheeses were manufactured using milk treated with mTG for different durations (30, 45 and 60 min) and assessed over 60 days. Longer treatment increased yield (18.79–20.81%) and hardness but reduced springiness. Composition, proteolysis and adhesiveness remained unaffected. Electrophoresis showed β-casein and αs1-casein declines during storage, with minor changes in αs1-casein's breakdown product. Microbial transglutaminase enzyme created a denser protein network, visible in SEM, resulting in a compact structure. Sensory analysis favoured the 60-min treatment. Overall, mTG improved Hatay cheese quality by modifying texture without major compositional changes.
这项研究旨在利用微生物转谷氨酰胺酶(mTG)改善哈塔伊奶酪的质地。使用经不同时间(30、45 和 60 分钟)mTG 处理的牛奶制作奶酪,并在 60 天内进行评估。较长时间的处理提高了产量(18.79-20.81%)和硬度,但降低了弹性。成分、蛋白质溶解度和粘附性则未受影响。电泳结果表明,β-酪蛋白和αs1-酪蛋白在贮藏过程中会下降,αs1-酪蛋白的分解产物变化不大。微生物转谷氨酰胺酶生成了更密集的蛋白质网络,在扫描电镜下可见,从而形成了紧凑的结构。感官分析更倾向于 60 分钟的处理。总的来说,mTG 通过改变质地提高了哈泰奶酪的质量,而没有发生重大的成分变化。
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引用次数: 0
Preliminary evaluation of Hyblean Ricotta cheese through seasons, a comparison of the chemical and sensory characteristics 对不同季节的海布伦乳清干酪进行初步评估,比较其化学和感官特征
IF 4.4 2区 农林科学 Q1 Agricultural and Biological Sciences Pub Date : 2024-01-01 DOI: 10.1111/1471-0307.13043
Guido Mangione, Margherita Caccamo, Kevin La Terra, Giovanni Marino, Antonio Difalco, Giuseppe Azzaro, Giovanni Belvedere, Giuseppe Licitra
The present work aimed at a preliminary characterisation of the Hyblean Ricotta cheese manufactured from semi-extensive dairies in Sicily. A survey on the detection of technological parameters during the production process, as well as the chemical and sensory analysis of raw material and the products, were performed to compare the effect of two different seasons linked with changes in the feeding system (pasture vs indoor). Results indicate that the Ricotta from pasture season showed a significantly higher fat, total solids and protein content and a higher yellow-colour appearance, bitter taste, vegetable odour and aroma compared to the indoor season samples.
本研究旨在对西西里岛半集约型奶牛场生产的海布伦乳清干酪进行初步特性分析。对生产过程中的技术参数检测以及原料和产品的化学和感官分析进行了调查,以比较两个不同季节与饲养系统(牧场与室内)变化的影响。结果表明,与室内季节的样品相比,牧场季节的意大利乳清干酪脂肪、总固形物和蛋白质含量明显更高,外观呈黄色,口感苦涩,有植物气味和香气。
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引用次数: 0
期刊
International Journal of Dairy Technology
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