International journal of biochemistry and molecular biology最新文献

Introduction: In Ghana, Corchorus olitorius, Solanum macrocarpon and Amaranthus cruentus are green leafy vegetables that are customarily eaten together with a starchy staple food. The present study aimed at assessing the ethanolic leaf extract of C. olitorius, S. macrocarpon and A. cruentus for antioxidant capacity, phytochemical property, nutritional and anti-nutrient content.

Method: Phytochemical constituent and proximate analysis were determined using standard protocols. The DPPH scavenging activity was used to determine the antioxidant activity of the ethanolic leaf extracts from the three vegetables. The antinutrients phytate and oxalate were determined by titrimetric methods of analysis.

Results: Pytochemical screening revealed the presence of tannins and flavonoids in C. olitorius, S. macrocarpon and A. cruentus. Alkaloids and saponins were present in C. olitorius and S. macrocarpon but not in A. cruentus. Terpenoids, steroids, carotenoids and coumarins were absent in all the three vegetables. Proximate analysis revealed varying levels of moisture, fat, protein, ash, crude fibre and carbohydrates in the three leafy vegetables. The DPPH scavenging showed 86.71%, 71.72% and 38.86% activity for S. macrocarpon, C. olitorius and A. cruentus respectively. The antinutrient results revealed an oxalate level of 2.7 ± 0.13% for C. olitorius, 6.43 ± 0.06% for A. cruentus and 12.32 ± 0.13% for S. macrocarpon. For levels of phytates, our results revealed a 3.084 ± 0.54%, 1.14 ± 0.26% and 1.71 ± 0.27% for C. olitorius, A. cruentus and S. macrocarpon, respectively.

Conclusion: The current study has shown that C. olitorius, A. cruentus and S. macrocarpon possess important phytochemicals, nutrients and significant antioxidant activity, suggesting a potential of these vegetables against diverse disease, if eaten by humans.

Background: Bloodstream infections are serious complications in neutropenic cancer patients. There has been a universal pickup in multidrug resistant (MDR) strains. For individuals who are at high risk for infections caused by MDR bacteria, a novel de-escalation strategy has been developed. Determine the bacterial spectrum and antibiotic resistance pattern in febrile neutropenic cancer patients was the goal of this investigation.

Materials and methods: From 2019 to 2020, 60 cancer patients with febrile neutropenia who were sent to Isfahan's Omid Hospital were included in this retrospective analysis. Experiments were done on the antimicrobial susceptibility of isolated bacterial infections.

Results: The patients' average age was 43.35±15.59 years. Ninety-one percent (55/61) of the 60 patients had hematologic malignancies, and 8.3 percent (5/61) had solid tumors. The majority of the germs were gram-negative bacteria (66.7 percent). E. coli was the pathogen that was isolated the most frequently (26.7%), followed by Klebsiella (16.7 percent). In addition, the most prevalent identified Gram-positive bacteria was Staphylococcus epidermidis (21.7 percent). Third-generation cephalosporin (ESBL-E) resistance was present in 50% of E. coli, along with 50% resistance to cotrimoxazole, ciprofloxacin, and piperacillin, 31% resistance to amikacin, and 20% resistance to meropenem (CRE). They had an 80% sensitivity to amikacin and a 70% sensitivity to ciprofloxacin. Ten percent of our patients had antibiotic resistance in the antibiogram (XDR).

Conclusion: In summary, most bacterial infections were resistant to different medications. The emergence and spread of Gram-negative bacteria that are resistant to antibiotics can be stopped by prudent antibiotic use.