Pub Date : 2019-07-01DOI: 10.33545/26646501.2019.v1.i2a.28
Aziz Ahmed, A. N. Soomro, Rehana Kauser, H. Basharat, M. Ramzan
{"title":"Evaluation of sperm mobility activation media for egg Fertiliztion in locally bred American channel catfish (Ictalurus punctatus)","authors":"Aziz Ahmed, A. N. Soomro, Rehana Kauser, H. Basharat, M. Ramzan","doi":"10.33545/26646501.2019.v1.i2a.28","DOIUrl":"https://doi.org/10.33545/26646501.2019.v1.i2a.28","url":null,"abstract":"","PeriodicalId":13891,"journal":{"name":"International journal of biochemistry and molecular biology","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75533155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-01DOI: 10.33545/26646501.2019.v1.i2a.9
A. Khaliq, Muhammad Abdul Shakoor, M. Yasin, N. Ahmad
{"title":"Occurrence of sugarcane yellow leaf virus in southern Punjab","authors":"A. Khaliq, Muhammad Abdul Shakoor, M. Yasin, N. Ahmad","doi":"10.33545/26646501.2019.v1.i2a.9","DOIUrl":"https://doi.org/10.33545/26646501.2019.v1.i2a.9","url":null,"abstract":"","PeriodicalId":13891,"journal":{"name":"International journal of biochemistry and molecular biology","volume":"80 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84105405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-01DOI: 10.33545/26646501.2019.v1.i2a.10
A. Hossain, A. M. Alsaif, R. M. Taha
The study was carried out to investigate the pigments (chlorophyll and anthocyanin), total phenol, flavonoid and mineral (K+) content in water apple fruit affected by gibberellic acid. The lowest amount of chlorophyll was observed in the control treatment. The highest chlorophyll content was found at 60 ppm GA3. The most effective concentration to earlier maturity of water apple fruit was 60 ppm of GA3. Moreover, flavonoid content was observed higher in 60 ppm GA3 than control, 30 and 90 ppm GA3 concentration. However, the most effective concentration for flavonoid content was in the 60 ppm GA3. Fruit K+ content was significantly increased by GA3 treatments, especially at 60 ppm. Anthocyanin compounds were found increasing trend while increasing the maturity development.
{"title":"Total phenol, flavonoid, anthoayanin and nutrient content development of water apple fruit affected by gibberellic acid","authors":"A. Hossain, A. M. Alsaif, R. M. Taha","doi":"10.33545/26646501.2019.v1.i2a.10","DOIUrl":"https://doi.org/10.33545/26646501.2019.v1.i2a.10","url":null,"abstract":"The study was carried out to investigate the pigments (chlorophyll and anthocyanin), total phenol, flavonoid and mineral (K+) content in water apple fruit affected by gibberellic acid. The lowest amount of chlorophyll was observed in the control treatment. The highest chlorophyll content was found at 60 ppm GA3. The most effective concentration to earlier maturity of water apple fruit was 60 ppm of GA3. Moreover, flavonoid content was observed higher in 60 ppm GA3 than control, 30 and 90 ppm GA3 concentration. However, the most effective concentration for flavonoid content was in the 60 ppm GA3. Fruit K+ content was significantly increased by GA3 treatments, especially at 60 ppm. Anthocyanin compounds were found increasing trend while increasing the maturity development.","PeriodicalId":13891,"journal":{"name":"International journal of biochemistry and molecular biology","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78187501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to investigate the antimitotic and antiproliferation activities of crude acetone-methanol and aqueous extracts of two marine molluscs commonly found in the Niger Delta region of Nigeria; T.fuscatus and P.aurita, against human cancerous cell lines (DU145, Hep-2, and HCC1395) cell lines in vitro. The antimitotic activity of the extracts was evaluated using Allium cepa root meristematic cells. Antiproliferative activity of the plant extracts against the cancerous cell lines was compared with normal cell line (VeroE6). Doxorubicin was used as a positive control. Gene expression studies using qPCR for the proapoptotic genes, CASP3, CASP8 and P53 were also carried out. The alcohol extract of T.fuscatus (TFAC) exhibited the most promising activity against all the cancer cell lines tested (DU145 IC50 = 96.48 ± 1.36 μg/ml, HCC 1395 IC50 = 61.44 ± 2.45 μg/ml, Hep2 IC50 = 0.52 ± 0.36 μg/ml) and also had the highest selectivity index of 4.94, 7.78 and 921.97 for DU145, HCC 1395 and Hep-2 cells respectively. Furthermore, TFAC was the only extract that significantly upregulated the expression of caspase 3, caspase 8 and P53. Thus, these findings suggest potential exploitation of TFAC as an anticancer agent.
{"title":"In vitro antiproliferative studies of extracts of the marine molluscs: Tympanatonus fuscatus Var radula (linnaeus) and Pachymelania aurita (muller).","authors":"Queensley Eghianruwa, Omolaja Osoniyi, Sabina Wachira, Naomi Maina, Regina Mbugua, Mabel Imbuga","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This study aimed to investigate the antimitotic and antiproliferation activities of crude acetone-methanol and aqueous extracts of two marine molluscs commonly found in the Niger Delta region of Nigeria; <i>T.fuscatus</i> and <i>P.aurita</i>, against human cancerous cell lines (DU145, Hep-2, and HCC1395) cell lines <i>in vitro</i>. The antimitotic activity of the extracts was evaluated using <i>Allium</i> cepa root meristematic cells. Antiproliferative activity of the plant extracts against the cancerous cell lines was compared with normal cell line (VeroE6). Doxorubicin was used as a positive control. Gene expression studies using qPCR for the proapoptotic genes, CASP3, CASP8 and P53 were also carried out. The alcohol extract of <i>T.fuscatus</i> (TFAC) exhibited the most promising activity against all the cancer cell lines tested (DU145 IC<sub>50</sub> = 96.48 ± 1.36 μg/ml, HCC 1395 IC<sub>50</sub> = 61.44 ± 2.45 μg/ml, Hep2 IC<sub>50</sub> = 0.52 ± 0.36 μg/ml) and also had the highest selectivity index of 4.94, 7.78 and 921.97 for DU145, HCC 1395 and Hep-2 cells respectively. Furthermore, TFAC was the only extract that significantly upregulated the expression of caspase 3, caspase 8 and P53. Thus, these findings suggest potential exploitation of TFAC as an anticancer agent.</p>","PeriodicalId":13891,"journal":{"name":"International journal of biochemistry and molecular biology","volume":"10 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2019-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6526377/pdf/ijbmb0010-0001.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37295695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.33545/26646501.2019.v1.i1a.12
D. Sivalingam
{"title":"A case study of physico: Chemical and biological parameters of adilabad district town lake, Andhra Pradesh, India","authors":"D. Sivalingam","doi":"10.33545/26646501.2019.v1.i1a.12","DOIUrl":"https://doi.org/10.33545/26646501.2019.v1.i1a.12","url":null,"abstract":"","PeriodicalId":13891,"journal":{"name":"International journal of biochemistry and molecular biology","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88320823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.33545/26646501.2019.v1.i1a.6
Dr. C.R. Subudhi, N. Jena, S. Suryavanshi, R. Subudhi
{"title":"Rainfall probability analysis for crop planning in Jagatsingpur district of Odisha, India","authors":"Dr. C.R. Subudhi, N. Jena, S. Suryavanshi, R. Subudhi","doi":"10.33545/26646501.2019.v1.i1a.6","DOIUrl":"https://doi.org/10.33545/26646501.2019.v1.i1a.6","url":null,"abstract":"","PeriodicalId":13891,"journal":{"name":"International journal of biochemistry and molecular biology","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82784451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.33545/26646501.2019.v1.i1a.2
L. Pandey, Satya Prakash Tantuway, Y. Bano, N. Swamy, Ranjan Singh, Sundaram Bhuvaneswari
{"title":"Developing a protocol for the isolation of high quality DNA from carbohydrate rich medicinal plant for molecular identification","authors":"L. Pandey, Satya Prakash Tantuway, Y. Bano, N. Swamy, Ranjan Singh, Sundaram Bhuvaneswari","doi":"10.33545/26646501.2019.v1.i1a.2","DOIUrl":"https://doi.org/10.33545/26646501.2019.v1.i1a.2","url":null,"abstract":"","PeriodicalId":13891,"journal":{"name":"International journal of biochemistry and molecular biology","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89101922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.33545/26646501.2019.v1.i1a.1
Ayaz Ahmad, Sahrish Khan
{"title":"Amelogenin (genetic marker) role in sex determination of human","authors":"Ayaz Ahmad, Sahrish Khan","doi":"10.33545/26646501.2019.v1.i1a.1","DOIUrl":"https://doi.org/10.33545/26646501.2019.v1.i1a.1","url":null,"abstract":"","PeriodicalId":13891,"journal":{"name":"International journal of biochemistry and molecular biology","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76596055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.33545/26646501.2019.v1.i1a.3
Dr. Veena Soni
{"title":"Renewable energy potential assessment in Indian perspective","authors":"Dr. Veena Soni","doi":"10.33545/26646501.2019.v1.i1a.3","DOIUrl":"https://doi.org/10.33545/26646501.2019.v1.i1a.3","url":null,"abstract":"","PeriodicalId":13891,"journal":{"name":"International journal of biochemistry and molecular biology","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76195915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-01DOI: 10.33545/26646501.2019.v1.i1a.4
Dr. Veena Soni
{"title":"Accelerating access to energy services: Way forward","authors":"Dr. Veena Soni","doi":"10.33545/26646501.2019.v1.i1a.4","DOIUrl":"https://doi.org/10.33545/26646501.2019.v1.i1a.4","url":null,"abstract":"","PeriodicalId":13891,"journal":{"name":"International journal of biochemistry and molecular biology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79613144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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