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in vitro screening the antibacterial activity of four whole body eusocial insects extracted by polar solvents 对极性溶剂提取的四种全社会性昆虫体外抗菌活性进行了筛选
International journal of biochemistry and molecular biology
Pub Date : 2021-01-01 DOI: 10.33545/26646501.2021.v3.i1a.21
A. Mekhlif
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引用次数: 1
Novel antibody against TMX2 and its effects on breast cancer cells. 新的抗TMX2抗体及其对乳腺癌细胞的影响。
International journal of biochemistry and molecular biology
Pub Date : 2020-02-15 eCollection Date: 2020-01-01
Eleana Hatzidaki, Dimitrios A Ntanovasilis, Ioannis Papasotiriou

Although monoclonal antibodies are promising, a truly fully human antibody is yet to be produced. Current human antibodies have the human sequence, but are produced in either transgenic animals or in phages. The aim of this paper was to produce a truly human antibody directed against an epitope of our choice, secreted by human plasma cells. The target protein was TMX2 one of the least studied disulfide isomerases. IgG and anti-TMX2 antibody were determined by both Elisa and western blot. TMX2 KD was evaluated by Surface Plasmon Resonance. TMX2 localization was determined by flow cytometry in MCF-7 cells. Efficiency was evaluated by MTT. Gene expression was evaluated by PCR. We have managed to produce two fully human antibodies directed against TMX2 protein. TMX2 protein was found both in the cytoplasm and cell membrane of breast cancer cells. RGCC TMX2 antibody recognizing an extracellular epitope increased cell proliferation. RGCC TMX2 antibody recognizing an intracellular epitope decreased cell proliferation and gene expression related to cancer survival, differentiation and metastasis. These findings suggest this platform is very promising for novel personalized therapies. TMX2 could be a novel target for cancer treatment.

虽然单克隆抗体很有前景，但真正的全人抗体尚未产生。目前的人类抗体具有人类的序列，但在转基因动物或噬菌体中产生。本文的目的是生产一种真正的人类抗体，直接针对我们选择的表位，由人类浆细胞分泌。靶蛋白是研究最少的二硫异构酶之一TMX2。Elisa和western blot检测血清中IgG和抗tmx2抗体。TMX2 KD采用表面等离子体共振法测定。流式细胞术检测TMX2在MCF-7细胞中的定位。采用MTT法评价效率。PCR检测基因表达。我们已经成功生产了两种针对TMX2蛋白的全人类抗体。TMX2蛋白存在于乳腺癌细胞的细胞质和细胞膜中。识别细胞外表位的RGCC TMX2抗体可促进细胞增殖。识别细胞内表位的RGCC TMX2抗体可降低肿瘤存活、分化和转移相关的细胞增殖和基因表达。这些发现表明，这个平台对于新的个性化治疗非常有希望。TMX2可能成为癌症治疗的新靶点。
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引用次数: 0
ABO and WCM biotypology: The secret of the holobiont ABO和WCM生物类型学:全息生物的秘密
International journal of biochemistry and molecular biology
Pub Date : 2020-01-01 DOI: 10.33545/26646501.2020.v2.i1a.17
M. Menapace
It has always been thought that we are just plain old human beings being composed of mere human eukaryotic cells and occasionally attacked by viruses and bacteria. But this vision is plainly wrong. We now know that we are a coherent mix of all kinds of life, running from unicellular microorganisms to pluricellular organisms, which form part and parcel of what can be called human being. We are “holobionts” and as such the multiplicity of cells and viruses belonging to all kingdoms of life collaborate in an equilibrium that if perturbed can cause disease. The ancient medicines (including Western Constitutional Medicine [WCM]) adopted this way of thinking and the ancient observers sagely imprinted their medicinal systems with this holistic view of life for which disease has to be contrasted with a return to equilibrium. And the ancient medicines used as primary tool the correction of the diet seen as the restoration of the equilibrium between the energies (microorganisms) in the body. More recently a new medicinal system the ABO groups has taken a stronger stand in the scientific arena through the experimental confirmation of its main mechanism of action by glycosciences (especially glycobiology). Necessarily, bacteria, fungi and other unicellular organisms can feed off on glycans which are both produced and absorbed by the human body (diet) and the suitable balance of diet will interfere in this cycle. It is now the time to capture all these advances in ancient and modern medicine and fuse them into a new medicinal system, the ABO-WCM biotypology, to better explain the reality of the holobiont and to completely transform modern science into a truly personalized approach.
人们一直认为，我们只是由真核细胞组成的普通人类，偶尔会受到病毒和细菌的攻击。但这种看法显然是错误的。我们现在知道，我们是各种生命的一个连贯的混合体，从单细胞微生物到多细胞生物，它们构成了所谓的人类的重要组成部分。我们是“全息生物”，因此，属于所有生命王国的细胞和病毒的多样性在一种平衡中合作，如果被扰乱就会导致疾病。古代医学(包括西方体质医学[WCM])采用了这种思维方式，古代的观察者明智地将这种整体的生命观烙印在他们的医学体系中，这种观点认为疾病必须与回归平衡相对照。古代医学作为主要工具，将饮食的调整视为恢复体内能量(微生物)之间的平衡。最近，通过糖科学(特别是糖生物学)对ABO血型的主要作用机制的实验证实，一个新的医疗系统在科学领域采取了更强有力的立场。细菌、真菌和其他单细胞生物必然会以人体(饮食)产生和吸收的聚糖为食，而适当的饮食平衡会干扰这一循环。现在是时候抓住古代和现代医学的所有这些进步，并将它们融合到一个新的医学系统中，即ABO-WCM生物类型学，以更好地解释全息生物的现实，并将现代科学完全转变为真正个性化的方法。
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引用次数: 0
Effects of N-benzyl-9-(2-tetrahydropyranyl) and indole-3-acetic acid in vitro culture of Butea buteiformis Voigt n -苄基-9-(2-四氢吡喃基)和吲哚-3-乙酸对丁茶离体培养的影响
International journal of biochemistry and molecular biology
Pub Date : 2020-01-01 DOI: 10.33545/26646501.2020.v2.i1a.16
Belai Meeta Suwal Singh
Butea buteiformis is a valuable tree with large trifoliate leaves with beautiful flower (Fig.1). It is distributed in tropical and subtropical region of Nepal. The seeds are bitter in taste and used for an anthelmintic. The plant possesses importance in agroforestry due to their nitrogen fixing capability. Plants are used for reforestration mostly on slope areas. The seeds were surface sterilized and cultured on half strength Murashige and Skoog (1962) (MS) medium. Nodal explants obtained from cultured were subcultured on different concentrations of N-Benzyl-9-(2-tetrahydropyranyl) (BPA) and Indole-3-acetic acid (IAA). The best proliferation of nodes and shoots were observed on the MS medium supplemented with 0.5 μM BPA and 0.5 μM IAA. After 8 weeks of culture the propagated plants were acclimatized and transferred to the sand box containing 1:1 soil and sand. Well rooted plants were then established in the field. All the data collected were worked out statistically with SPSS, a system of analytical procedure.
Butea buteiformis是一种珍贵的乔木，叶大，三叶状，花漂亮(图1)。它分布在尼泊尔的热带和亚热带地区。这种种子的味道是苦的，用来做驱虫药。该植物因其固氮能力在农林业中具有重要意义。植物主要用于斜坡地区的再造林。对种子进行表面灭菌，并在半强度Murashige和Skoog (1962) (MS)培养基上培养。用不同浓度的n -苄基-9-(2-四氢吡喃基)(BPA)和吲哚-3-乙酸(IAA)进行继代培养。在添加0.5 μM BPA和0.5 μM IAA的MS培养基上，茎节和芽的增殖效果最好。培养8周后，将繁殖植株移栽到土砂1:1的砂箱中。然后在地里种上生根良好的植物。所有收集到的数据都用SPSS(一种分析程序系统)进行统计。
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引用次数: 0
Studies of heavy metal content in dates, grapes, pineapple, rambutan, apple, banana and mango as fruit pollutant 红枣、葡萄、菠萝、红毛丹、苹果、香蕉、芒果等水果污染物重金属含量的研究
International journal of biochemistry and molecular biology
Pub Date : 2020-01-01 DOI: 10.33545/26646501.2020.v2.i1a.14
A. Hossain
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引用次数: 0
Root, shoot and callus cell proliferation from broccoli root tip, shoot tip and leaf cutting in vitro 西兰花根尖、茎尖和离体切叶的根、茎和愈伤组织细胞增殖
International journal of biochemistry and molecular biology
Pub Date : 2020-01-01 DOI: 10.33545/26646501.2020.v2.i1a.13
A. Hossain, M. M. Uddin
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引用次数: 0
Benefits of camellia sinensis 山茶的好处
International journal of biochemistry and molecular biology
Pub Date : 2019-07-01 DOI: 10.33545/26646501.2019.v1.i2a.11
Ashikujaman Syed
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引用次数: 0
in vitro callus induction of safflower (Carthimus tinctorius L.) 红花离体愈伤组织诱导的研究
International journal of biochemistry and molecular biology
Pub Date : 2019-07-01 DOI: 10.33545/26646501.2019.v1.i2a.8
NS Pawar, B. Rathod, P. K. Majhi, VE Gotamwad
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引用次数: 0
Inhibitory effect of leaf and root bark of Calotropis procera and Parquetina nigrescens on larval midgut enzyme activities of Callosobruchus maculatus 牛角参叶、根皮和黑皮对斑点胼胝体幼虫中肠酶活性的抑制作用
International journal of biochemistry and molecular biology
Pub Date : 2019-07-01 DOI: 10.33545/26646501.2019.v1.i2a.5
Alamuoye Nathaniel Olu
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引用次数: 0
Utilization of hormones as cell signaling component, its mechanism and regulated protein and gene expression 激素作为细胞信号传导成分的利用及其机制及调控的蛋白和基因表达
International journal of biochemistry and molecular biology
Pub Date : 2019-07-01 DOI: 10.33545/26646501.2019.v1.i2a.7
A. Hossain, M. M. Uddin
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引用次数: 0
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