Pub Date : 2019-06-15DOI: 10.38111/ijapb.20190502002
G. Rachana, B. Subrahmanyam, N. Prashanthi, B. Sharvanabhava
{"title":"A Case Report on Early Diagnosis of Brain Tuberculoma in an Adolescent","authors":"G. Rachana, B. Subrahmanyam, N. Prashanthi, B. Sharvanabhava","doi":"10.38111/ijapb.20190502002","DOIUrl":"https://doi.org/10.38111/ijapb.20190502002","url":null,"abstract":"","PeriodicalId":13904,"journal":{"name":"International Journal of Advances in Pharmacy and Biotechnology","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78104601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-15DOI: 10.38111/ijapb.20190502001
Yamini Kumara Tadikonda, M. Dhanalakshmi
{"title":"RP-HPLC Method Development and Validation of Rofecoxib in Bulk and Dosage Form","authors":"Yamini Kumara Tadikonda, M. Dhanalakshmi","doi":"10.38111/ijapb.20190502001","DOIUrl":"https://doi.org/10.38111/ijapb.20190502001","url":null,"abstract":"","PeriodicalId":13904,"journal":{"name":"International Journal of Advances in Pharmacy and Biotechnology","volume":"118 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77292475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-03-15DOI: 10.38111/ijapb.20190501002
Md. Asra Farheen, M. Dhanalakshmi, Yamini Kumara Tadikonda
{"title":"RP-HPLC Method for Determination of Zinostatin in Bulk and Pharmaceutical Formulation","authors":"Md. Asra Farheen, M. Dhanalakshmi, Yamini Kumara Tadikonda","doi":"10.38111/ijapb.20190501002","DOIUrl":"https://doi.org/10.38111/ijapb.20190501002","url":null,"abstract":"","PeriodicalId":13904,"journal":{"name":"International Journal of Advances in Pharmacy and Biotechnology","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85037277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-03-15DOI: 10.38111/ijapb.20190501001
D. Girija, Basu Venkateswara Reddy, K. Kamalakar, C. Manasa, B. Pavani
{"title":"A Review on Tracer Technique and Its Applications in Synthesis of Secondary Metabolites in Pharmacognosy","authors":"D. Girija, Basu Venkateswara Reddy, K. Kamalakar, C. Manasa, B. Pavani","doi":"10.38111/ijapb.20190501001","DOIUrl":"https://doi.org/10.38111/ijapb.20190501001","url":null,"abstract":"","PeriodicalId":13904,"journal":{"name":"International Journal of Advances in Pharmacy and Biotechnology","volume":"2015 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87797661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-01-27DOI: 10.38111/ijapb.20190501003
Yashwanth Kumar K, M. K, V. E, Sharavanabhava B.S
An phenytoin is commonly administered as prophylactic or treatment of epileptic episodes in acute brain injury due to head injury. The aim of the study is to evaluate PK pattern of phenytoin in patients with traumatic and non-traumatic brain injuries. This study was carried out in 30 adult head injury patients and who were administered with phenytoin for prophylaxis of post trauma seizures or treatment. Serum Phenytoin concentrations (Cp) were determined and were compared between CVA and HT patients. The Km and Vmax were significantly higher in HT patients. The Cp and the Cp/dose ratio were higher in the CVA patients significantly (P<0.05). APACHE П score was significantly lower than the baseline at the end of the study in each group of patients (P<0.05). Due to significant differences in Cp and PK parameters between HT and CVA patients, close attention must be paid to the PK behavior of phenytoin in the efforts to improve the patient’s outcome after a severe HT.
{"title":"A Prospective Observational Study: Phenytoin Pharmacokinetic Pattern in Cerebrovascular Accident and Head Trauma Patients in Warangal Population","authors":"Yashwanth Kumar K, M. K, V. E, Sharavanabhava B.S","doi":"10.38111/ijapb.20190501003","DOIUrl":"https://doi.org/10.38111/ijapb.20190501003","url":null,"abstract":"An phenytoin is commonly administered as prophylactic or treatment of epileptic episodes in acute brain injury due to head injury. The aim of the study is to evaluate PK pattern of phenytoin in patients with traumatic and non-traumatic brain injuries. This study was carried out in 30 adult head injury patients and who were administered with phenytoin for prophylaxis of post trauma seizures or treatment. Serum Phenytoin concentrations (Cp) were determined and were compared between CVA and HT patients. The Km and Vmax were significantly higher in HT patients. The Cp and the Cp/dose ratio were higher in the CVA patients significantly (P<0.05). APACHE П score was significantly lower than the baseline at the end of the study in each group of patients (P<0.05). Due to significant differences in Cp and PK parameters between HT and CVA patients, close attention must be paid to the PK behavior of phenytoin in the efforts to improve the patient’s outcome after a severe HT.","PeriodicalId":13904,"journal":{"name":"International Journal of Advances in Pharmacy and Biotechnology","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82214250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-15DOI: 10.38111/ijapb.20180402002
R. Gunda, Prasada Rao Manchineni, MV Kiran Kumar, G. K. Rao
The main aim of present research study is to formulate the floating tablets of Labetalol HCl using 32 factorial design. Labetalol HCl, non selective α, -βadreno receptor antagonist, Indicated for treatment of Hypertension/moderate Heart Failure. The Floating tablets of Labetalol HCl were prepared employing different concentrations of HPMCK4M and sodium bicarbonate in different combinations by Direct Compression technique. The concentration of HPMCK4M and sodium bicarbonate required to achieve desired drug release was selected as independent variables, X1 and X2 respectively whereas, time required for 10% of drug dissolution (t10%), 50% (t50%), 75% (t75%) and 90% (t90%) were selected as dependent variables. 9 formulations were designed and are evaluated for hardness, friability, thickness, % drug content, Floating Lag time, In-vitro drug release. From the Results concluded that all the formulation were found to be within the Pharmacopoeial limits and the In-vitro dissolution profiles of all formulations were fitted in to different Kinetic models, the statistical parameters like intercept, slope & regression coefficient were calculated. Polynomial equations were developed for t10%, t50%, t75%, t90%. Validity of developed polynomial equations were verified by designing 2 check point formulations. According to SUPAC guidelines the formulation (F8) containing combination of 20% HPMCK4M and 3.75% sodium bicarbonate, is the most identical formulation which meets the objective of work. The selected formulation (F8) follows Higuchi’s kinetics, and the mechanism of drug release was found to be NonFickian Diffusion (n= 1.033, Super Case-II transport).
本研究的主要目的是采用32因子设计对盐酸拉贝他洛尔漂浮片进行配方研究。盐酸拉贝他洛尔，非选择性α， β肾上腺素受体拮抗剂，用于治疗高血压/中度心力衰竭。以不同浓度的HPMCK4M和碳酸氢钠为原料，采用直接压缩法制备盐酸拉贝他洛尔漂浮片。分别以达到预期药物释放所需的HPMCK4M浓度和碳酸氢钠浓度为自变量X1和X2，以药物溶出10% (t10%)、50% (t50%)、75% (t75%)和90% (t90%)所需的时间为因变量。设计了9种制剂，对其硬度、脆度、厚度、药物含量、漂浮滞后时间、体外释放度进行了评价。结果表明，各制剂均在药典限定范围内，并拟合了各制剂的体外溶出度曲线，计算了其截距、斜率、回归系数等统计参数。对t10%、t50%、t75%、t90%分别建立了多项式方程。通过设计2个检查点公式，验证了所建立的多项式方程的有效性。根据SUPAC指南，含有20% HPMCK4M和3.75%碳酸氢钠的配方(F8)是最符合工作目标的配方。所选制剂(F8)符合Higuchi动力学，药物释放机制为非菲克扩散(n= 1.033, Super Case-II转运)。
{"title":"DESIGN, DEVELOPMENT AND EVALUATION OF LABETALOL HCl GASTRO RETENTIVE FLOATING TABLETS","authors":"R. Gunda, Prasada Rao Manchineni, MV Kiran Kumar, G. K. Rao","doi":"10.38111/ijapb.20180402002","DOIUrl":"https://doi.org/10.38111/ijapb.20180402002","url":null,"abstract":"The main aim of present research study is to formulate the floating tablets of Labetalol HCl using 32 factorial design. Labetalol HCl, non selective α, -βadreno receptor antagonist, Indicated for treatment of Hypertension/moderate Heart Failure. The Floating tablets of Labetalol HCl were prepared employing different concentrations of HPMCK4M and sodium bicarbonate in different combinations by Direct Compression technique. The concentration of HPMCK4M and sodium bicarbonate required to achieve desired drug release was selected as independent variables, X1 and X2 respectively whereas, time required for 10% of drug dissolution (t10%), 50% (t50%), 75% (t75%) and 90% (t90%) were selected as dependent variables. 9 formulations were designed and are evaluated for hardness, friability, thickness, % drug content, Floating Lag time, In-vitro drug release. From the Results concluded that all the formulation were found to be within the Pharmacopoeial limits and the In-vitro dissolution profiles of all formulations were fitted in to different Kinetic models, the statistical parameters like intercept, slope & regression coefficient were calculated. Polynomial equations were developed for t10%, t50%, t75%, t90%. Validity of developed polynomial equations were verified by designing 2 check point formulations. According to SUPAC guidelines the formulation (F8) containing combination of 20% HPMCK4M and 3.75% sodium bicarbonate, is the most identical formulation which meets the objective of work. The selected formulation (F8) follows Higuchi’s kinetics, and the mechanism of drug release was found to be NonFickian Diffusion (n= 1.033, Super Case-II transport).","PeriodicalId":13904,"journal":{"name":"International Journal of Advances in Pharmacy and Biotechnology","volume":"72 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85922157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-15DOI: 10.38111/ijapb.20180402004
Urvashi B. Patel, Harshil M. Patel, Chainesh N. Shah
To obtain an enhanced in-vitro dissolution rate of simvastatin by using Liquisolid technique and Liquisolid tablets were optimized by DoE approach 32 full factorial design using Design Expert Software.Theliquisolid tablets were formulated by using propylene glycol (PG), as liquid vehicle, Avicel PH-102 as a carrier material, Aerosil as a coating material, and aspartame as sweetener and Kyron 314 as a superdisintegrant. The new mathematical model 32 full factorial design was utilized to formulate various liquisolid powder systems and to calculate amount of carrier material and coating material. All prepared liquisolid batches were subjected to weight variation, drug content uniformity, hardness, friability test, and disintegration test and dissolution tests. Liquisolid systems were also tested for DSC, FT-IR. From result of check point analysis of design data, , SMLCFDT10 shows higher Drug release (89.257 %) at less wetting time (124.682 sec.) and disintegrating time (31.843 sec). Simvasatin liquisolid compacts enhance aqueous solubility and dissolution rate in compare to other solubility enhancement technique. Hence, this research work may be useful to formulate fast disintegrating Tablets using Liquisolid Technique which may give rapid onset of action by rapid absorption, maximize efficacy, reduce dose and dose frequency & hence increase patient Compliance.
{"title":"FORMULATION AND EVALUATION OF FAST DISINTEGRATING TABLETS OF SIMVASTATIN USING LIQUISOLID TECHNOLOGY BY USING DOE APPROACH","authors":"Urvashi B. Patel, Harshil M. Patel, Chainesh N. Shah","doi":"10.38111/ijapb.20180402004","DOIUrl":"https://doi.org/10.38111/ijapb.20180402004","url":null,"abstract":"To obtain an enhanced in-vitro dissolution rate of simvastatin by using Liquisolid technique and Liquisolid tablets were optimized by DoE approach 32 full factorial design using Design Expert Software.Theliquisolid tablets were formulated by using propylene glycol (PG), as liquid vehicle, Avicel PH-102 as a carrier material, Aerosil as a coating material, and aspartame as sweetener and Kyron 314 as a superdisintegrant. The new mathematical model 32 full factorial design was utilized to formulate various liquisolid powder systems and to calculate amount of carrier material and coating material. All prepared liquisolid batches were subjected to weight variation, drug content uniformity, hardness, friability test, and disintegration test and dissolution tests. Liquisolid systems were also tested for DSC, FT-IR. From result of check point analysis of design data, , SMLCFDT10 shows higher Drug release (89.257 %) at less wetting time (124.682 sec.) and disintegrating time (31.843 sec). Simvasatin liquisolid compacts enhance aqueous solubility and dissolution rate in compare to other solubility enhancement technique. Hence, this research work may be useful to formulate fast disintegrating Tablets using Liquisolid Technique which may give rapid onset of action by rapid absorption, maximize efficacy, reduce dose and dose frequency & hence increase patient Compliance.","PeriodicalId":13904,"journal":{"name":"International Journal of Advances in Pharmacy and Biotechnology","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87386393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-15DOI: 10.38111/ijapb.20180402003
T. Kalpana, M. Madhavi, P. Mounika Rani, R. Kavitha, M. Manisha, K. Kalyani
Received: 18 August 2018 Revised: 24 August 2018 Accepted: 29 August 2018 The objective of the current research work is to investigate the in-vitro antibacterial and anthelmintic activity.The combined tri-herbal methanolic extract made up of equal quantities of leaves of Moringa oleifera, seeds of Terminalia chebula, and fresh bulbs of Allium sativum was evaluated for its in-vitro antibacterial and anthelmintic activity and was compared with its individual methanolic extracts of Terminalia chebula. The anti-bacterial activity was evaluated against gram neagative and gram positive bacteria. Streptomycin was used as a standard drug. The Anthelmintic activity was evaluated against Pheretima posthuma. Albendazole was used as a standard drug. The experimental results showed that triherbal methanolic plant extracts possess better activity on both microorganisms and earthworms. The combined activity of Moringa oleifera, Terminalia chebula and Allium sativum has been reported for the first time.
{"title":"IN-VITRO ANTI BACTERIAL AND ANTHELMINTIC ACTIVITY OF TERMINALIA CHEBULA, MORINGA OLEIFERA AND ALLIUM SATIVUM","authors":"T. Kalpana, M. Madhavi, P. Mounika Rani, R. Kavitha, M. Manisha, K. Kalyani","doi":"10.38111/ijapb.20180402003","DOIUrl":"https://doi.org/10.38111/ijapb.20180402003","url":null,"abstract":"Received: 18 August 2018 Revised: 24 August 2018 Accepted: 29 August 2018 The objective of the current research work is to investigate the in-vitro antibacterial and anthelmintic activity.The combined tri-herbal methanolic extract made up of equal quantities of leaves of Moringa oleifera, seeds of Terminalia chebula, and fresh bulbs of Allium sativum was evaluated for its in-vitro antibacterial and anthelmintic activity and was compared with its individual methanolic extracts of Terminalia chebula. The anti-bacterial activity was evaluated against gram neagative and gram positive bacteria. Streptomycin was used as a standard drug. The Anthelmintic activity was evaluated against Pheretima posthuma. Albendazole was used as a standard drug. The experimental results showed that triherbal methanolic plant extracts possess better activity on both microorganisms and earthworms. The combined activity of Moringa oleifera, Terminalia chebula and Allium sativum has been reported for the first time.","PeriodicalId":13904,"journal":{"name":"International Journal of Advances in Pharmacy and Biotechnology","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86628491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-15DOI: 10.38111/ijapb.20180402001
Mohsina Fp, Faheem Ip, S. Priya, Shaikh Mohammed Azhar Husain
The plant Ichnocarpus frutescens L. is from the family Apocynaceae, which extensively cultivated in most regions of the world and common avenue tree, commonly called as black creeper in English .The literature survey reveals that Ichnocarpus frutescens L.roots has been used as tonic, diuretic, demulcent, diaphoretic and anti diabetic.Young stems and leaves are also used for diabetes. Young stems and leaves contain triterpenoids, α –amyrin, acetalupeol and its acetates, friedelin, epifriedelinol and β-sitosterol. Flowers and fruits contain flavonoids-Quercetin, Kaepferol-3-glucoside. The Ethanolic and Aqueous extracts of dried flowers of Ichnocarpus frutescens L.were screened for anti-diabetic activity in normal rats, STZ induced diabetic rats and on serum glucose levels in glucose over loaded rats. The effect on the insulin level with treatment by aqueous and ethanolic extract of Ichnocarpus frutescens L. flowers suggest that the mode of action is similar to that of glibenclamide. Oral administration of both the extracts for 21 days significantly reduced blood glucose level in STZ induced diabetic rats. Both the extracts exhibited antihyperglycemic effect in glucose loaded rats and STZ induced rats.
{"title":"EVALUATION OF ANTI DIABETIC ACTIVITY OF ICHNOCARPUS FRUTESCENS L.","authors":"Mohsina Fp, Faheem Ip, S. Priya, Shaikh Mohammed Azhar Husain","doi":"10.38111/ijapb.20180402001","DOIUrl":"https://doi.org/10.38111/ijapb.20180402001","url":null,"abstract":"The plant Ichnocarpus frutescens L. is from the family Apocynaceae, which extensively cultivated in most regions of the world and common avenue tree, commonly called as black creeper in English .The literature survey reveals that Ichnocarpus frutescens L.roots has been used as tonic, diuretic, demulcent, diaphoretic and anti diabetic.Young stems and leaves are also used for diabetes. Young stems and leaves contain triterpenoids, α –amyrin, acetalupeol and its acetates, friedelin, epifriedelinol and β-sitosterol. Flowers and fruits contain flavonoids-Quercetin, Kaepferol-3-glucoside. The Ethanolic and Aqueous extracts of dried flowers of Ichnocarpus frutescens L.were screened for anti-diabetic activity in normal rats, STZ induced diabetic rats and on serum glucose levels in glucose over loaded rats. The effect on the insulin level with treatment by aqueous and ethanolic extract of Ichnocarpus frutescens L. flowers suggest that the mode of action is similar to that of glibenclamide. Oral administration of both the extracts for 21 days significantly reduced blood glucose level in STZ induced diabetic rats. Both the extracts exhibited antihyperglycemic effect in glucose loaded rats and STZ induced rats.","PeriodicalId":13904,"journal":{"name":"International Journal of Advances in Pharmacy and Biotechnology","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87089409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-11-16DOI: 10.38111/ijapb.20180404003
N. R. Babu, Syeda Sana Mahveen, Y. Padmavathi, Ananddas Ramesh Priyadharshini, P. Ravi Kumar, A. Divya, Anil Kumar Tallam
A new visible spectrophotometric method was developed for the determination of paraphenylenediamine (PPD) in pure and marketed forms using β‐naphthol as chromogenic reagent. This is based on reaction of PPD with β‐naphthol in acidic media to yield reddish‐pink coloured chromogen exhibiting absorption maximum at 552.4 nm. Beer’s law is obeyed in the concentration range of 200‐1000 ng/mL with coefficient of determination as 0.9976. The limit of detection (LOD) and limit of quantitation (LOQ) were found to be 64.2 ng/mL and 194.8 ng/mL respectively. The developed method has been validated as per the ICH Q2 (R1) guidelines. The results demonstrate that the method is linear, precise and accurate. The proposed method was successfully applied for determination of PPD in different brands of hair dyes with good recovery and reproducibility.
{"title":"SPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF PARA PHENYLENEDIAMINE IN HAIR DYES","authors":"N. R. Babu, Syeda Sana Mahveen, Y. Padmavathi, Ananddas Ramesh Priyadharshini, P. Ravi Kumar, A. Divya, Anil Kumar Tallam","doi":"10.38111/ijapb.20180404003","DOIUrl":"https://doi.org/10.38111/ijapb.20180404003","url":null,"abstract":"A new visible spectrophotometric method was developed for the determination of paraphenylenediamine (PPD) in pure and marketed forms using β‐naphthol as chromogenic reagent. This is based on reaction of PPD with β‐naphthol in acidic media to yield reddish‐pink coloured chromogen exhibiting absorption maximum at 552.4 nm. Beer’s law is obeyed in the concentration range of 200‐1000 ng/mL with coefficient of determination as 0.9976. The limit of detection (LOD) and limit of quantitation (LOQ) were found to be 64.2 ng/mL and 194.8 ng/mL respectively. The developed method has been validated as per the ICH Q2 (R1) guidelines. The results demonstrate that the method is linear, precise and accurate. The proposed method was successfully applied for determination of PPD in different brands of hair dyes with good recovery and reproducibility.","PeriodicalId":13904,"journal":{"name":"International Journal of Advances in Pharmacy and Biotechnology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88823453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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