Pub Date : 2020-02-18DOI: 10.11603/ijmmr.2413-6077.2019.2.10698
O. Yaremchuk, K. Posokhova, I. Kuzmak, M. Kulitska, О. О. Shevchuk, A. Volska, P. Lykhatskyi
Background. Antiphospholipid syndrome (APS) is an autoimmune disease characterized by the presence of specific antibodies. Objective. The aim of the study was to investigate the effect of combined use of L-arginine and aminoguanidine on cytokine profile (IL-1β, IL-6, TNF-α, IL-4, IL-10) in experimental APS. Methods. The study was performed on BALB/c female mice. L-arginine (25 mg/kg) and aminoguanidine (10 mg/kg) were used for correction. Serum cytokines concentrations were assessed using an ELISA test. Results. It was found that in APS the concentration of proinflammatory cytokines IL-1β, IL-6 and TNF-a increases in 3.2, 2.3 and 4.5 times respectively, compare to the control. At the same time a decrease of the IL-4 and IL-10 in 1.9 and 2.2 times was evidenced. Aminoguanidine, a selective iNOS inhibitor, caused a significant decrease of TNF-α by 57% (p<0.001), but there were no changes in IL-1β, IL-6, IL-4 and IL-10 compare to the APS-group. L-arginine combined with aminoguanidine caused a significant decrease in the concentration of IL-1β by 30% (p<0.01), IL-6 – by 16% (p<0.05), TNF-a – by 59% (p<0.001) compare to the control. At the same time, the concentration of IL-4 increased by 35% (p <0.01), IL-10 – by 25% (p<0.005). Conclusions. Combined use of the precursor of the NO synthesis L-arginine and aminoguanidine, a selective iNOS inhibitor, leads to a decrease in the concentrations of IL-1β, IL-6, TNF-a and an increase of IL-4 and IL-10 compare to the group of the BALB/c mice with APS and the group of animals administered with aminoguanidine.
{"title":"IMPACT OF NITRIC OXIDE SYNTHESIS MODULATORS ON THE CYTOKINES PROFILE IN EXPERIMENTAL ANTIPHOSPHOLIPID SYNDROME","authors":"O. Yaremchuk, K. Posokhova, I. Kuzmak, M. Kulitska, О. О. Shevchuk, A. Volska, P. Lykhatskyi","doi":"10.11603/ijmmr.2413-6077.2019.2.10698","DOIUrl":"https://doi.org/10.11603/ijmmr.2413-6077.2019.2.10698","url":null,"abstract":"Background. Antiphospholipid syndrome (APS) is an autoimmune disease characterized by the presence of specific antibodies. Objective. The aim of the study was to investigate the effect of combined use of L-arginine and aminoguanidine on cytokine profile (IL-1β, IL-6, TNF-α, IL-4, IL-10) in experimental APS. Methods. The study was performed on BALB/c female mice. L-arginine (25 mg/kg) and aminoguanidine (10 mg/kg) were used for correction. Serum cytokines concentrations were assessed using an ELISA test. Results. It was found that in APS the concentration of proinflammatory cytokines IL-1β, IL-6 and TNF-a increases in 3.2, 2.3 and 4.5 times respectively, compare to the control. At the same time a decrease of the IL-4 and IL-10 in 1.9 and 2.2 times was evidenced. Aminoguanidine, a selective iNOS inhibitor, caused a significant decrease of TNF-α by 57% (p<0.001), but there were no changes in IL-1β, IL-6, IL-4 and IL-10 compare to the APS-group. L-arginine combined with aminoguanidine caused a significant decrease in the concentration of IL-1β by 30% (p<0.01), IL-6 – by 16% (p<0.05), TNF-a – by 59% (p<0.001) compare to the control. At the same time, the concentration of IL-4 increased by 35% (p <0.01), IL-10 – by 25% (p<0.005). Conclusions. Combined use of the precursor of the NO synthesis L-arginine and aminoguanidine, a selective iNOS inhibitor, leads to a decrease in the concentrations of IL-1β, IL-6, TNF-a and an increase of IL-4 and IL-10 compare to the group of the BALB/c mice with APS and the group of animals administered with aminoguanidine.","PeriodicalId":14059,"journal":{"name":"International Journal of Medicine and Medical Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44029239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-18DOI: 10.11603/ijmmr.2413-6077.2019.2.10459
Y. Lekomtseva
Background. Mild traumatic brain injury (mTBI) leads to disturbance of various metabolic processes significant in pathogenesis of the maintaining of long-term consequences after it. The objective of the research was to analyse changes in the activity of some membrane-associated enzyme markers, which are involved in different redox reactions, reflecting main metabolic processes. Methods. Forty-seven patients with long-term consequences of mTBI, thirty controls were enrolled. The levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase (LDH), gamma-glutamyl transpeptidase were evaluated in sera by gas-liquid chromatograph and calorimetric methods. Results. The study revealed significant changes in metabolic processes observed for alkaline phosphatase and LDH, which were the indicators of membrane and redox processes disturbances, acidosis severity and impaired energy cell metabolism. The averages of LDH level was 662.7 versus 381.9 U/L, in the controls. The disease progression was followed by directly proportional LDH increase reaching very high values in the patients with disease duration more than 15 years (mean ±SD 144.6±16.3 versus 82.6±8.4 U/L, controls p<0.05). The long-term consequences of mTBI were characterized by statistically significant decrease of alkaline phosphatase and positive dependence (p<0.05) of it (r=+0.48) on the disease duration with the averages of alkaline phosphatase level of 152.5±11.21 versus 212.6±9.63 U/L, controls (p<0.01). The significance of changes in membrane-associated enzymes serum levels correlated with development of oxidative stress and metabolic processes dysfunction. Conclusion. In the patients with long-term consequences of mTBI, dysregulation of enzymes activity was detected that might be a marker of nervous system energy impairment and membranes destruction.
{"title":"SOME METABOLIC PROCESSES IN THE PATIENTS WITH LONG-TERM CONSEQUENCES OF MILD TRAUMATIC BRAIN INJURY","authors":"Y. Lekomtseva","doi":"10.11603/ijmmr.2413-6077.2019.2.10459","DOIUrl":"https://doi.org/10.11603/ijmmr.2413-6077.2019.2.10459","url":null,"abstract":"Background. Mild traumatic brain injury (mTBI) leads to disturbance of various metabolic processes significant in pathogenesis of the maintaining of long-term consequences after it. The objective of the research was to analyse changes in the activity of some membrane-associated enzyme markers, which are involved in different redox reactions, reflecting main metabolic processes. Methods. Forty-seven patients with long-term consequences of mTBI, thirty controls were enrolled. The levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase (LDH), gamma-glutamyl transpeptidase were evaluated in sera by gas-liquid chromatograph and calorimetric methods. Results. The study revealed significant changes in metabolic processes observed for alkaline phosphatase and LDH, which were the indicators of membrane and redox processes disturbances, acidosis severity and impaired energy cell metabolism. The averages of LDH level was 662.7 versus 381.9 U/L, in the controls. The disease progression was followed by directly proportional LDH increase reaching very high values in the patients with disease duration more than 15 years (mean ±SD 144.6±16.3 versus 82.6±8.4 U/L, controls p<0.05). The long-term consequences of mTBI were characterized by statistically significant decrease of alkaline phosphatase and positive dependence (p<0.05) of it (r=+0.48) on the disease duration with the averages of alkaline phosphatase level of 152.5±11.21 versus 212.6±9.63 U/L, controls (p<0.01). The significance of changes in membrane-associated enzymes serum levels correlated with development of oxidative stress and metabolic processes dysfunction. Conclusion. In the patients with long-term consequences of mTBI, dysregulation of enzymes activity was detected that might be a marker of nervous system energy impairment and membranes destruction.","PeriodicalId":14059,"journal":{"name":"International Journal of Medicine and Medical Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46157087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-18DOI: 10.11603/ijmmr.2413-6077.2019.2.10458
Syed Asif Hashmi, G. Bhadauria, K. Rajmohan, Inam Danish Khan, A. Gupta, D. Mitra, R. Gupta, Mehreen Rahman, U. Kapoor, S. Singh, F. M. Ahmad
S.A. Hashmi1, G.S. Bhadauria1, K.S. Rajmohan1, *I.D. Khan1, A. Gupta1, D. Mitra1, R.M. Gupta2, M. Rahman3, U. Kapoor4, S.K. Singh5, F.M.H. Ahmad1 1 – ARMY COLLEGE OF MEDICAL SCIENCES AND BASE HOSPITAL, NEW DELHI, INDIA 2 – ARMED FORCES MEDICAL COLLEGE, PUNE, INDIA 3 – BUDDHA INSTITUTE OF DENTAL SCIENCES, INDIA 4 – COMMAND HOSPITAL (NC), UDHAMPUR, INDIA 5 – ALL INDIA INSTITUTE OF MEDICAL SCIENCES, NEW DELHI, INDIA
{"title":"HANSEN’S DISEASE DIAGNOSED AFTER ANTI-CANCER CHEMOTHERAPY","authors":"Syed Asif Hashmi, G. Bhadauria, K. Rajmohan, Inam Danish Khan, A. Gupta, D. Mitra, R. Gupta, Mehreen Rahman, U. Kapoor, S. Singh, F. M. Ahmad","doi":"10.11603/ijmmr.2413-6077.2019.2.10458","DOIUrl":"https://doi.org/10.11603/ijmmr.2413-6077.2019.2.10458","url":null,"abstract":"S.A. Hashmi1, G.S. Bhadauria1, K.S. Rajmohan1, *I.D. Khan1, A. Gupta1, D. Mitra1, R.M. Gupta2, M. Rahman3, U. Kapoor4, S.K. Singh5, F.M.H. Ahmad1 1 – ARMY COLLEGE OF MEDICAL SCIENCES AND BASE HOSPITAL, NEW DELHI, INDIA 2 – ARMED FORCES MEDICAL COLLEGE, PUNE, INDIA 3 – BUDDHA INSTITUTE OF DENTAL SCIENCES, INDIA 4 – COMMAND HOSPITAL (NC), UDHAMPUR, INDIA 5 – ALL INDIA INSTITUTE OF MEDICAL SCIENCES, NEW DELHI, INDIA","PeriodicalId":14059,"journal":{"name":"International Journal of Medicine and Medical Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42485616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-18DOI: 10.11603/ijmmr.2413-6077.2019.2.10671
O. I. Zarudna, I. Venher, A. Dovbush
Background. A microcirculatory bloodstream is a target, source and reason of the pathological process in patients with systemic connective tissue diseases. Objectives. This study is focused on meta-analyses of biopsy material of skin flaps harvested from patients’ fingers to identify specific morphological changes. Methods. A retrospective analysis of the medical records of 39 examinees with systemic sclerosis (SSc), 45 with Systemic Lupus Erythematosus (SLE), and 45 with rheumatoid arthritis (RA) was performed. The condition of peripheral hemodynamics was examined with longitudinal rheovasography of arms and legs. Endothelin-1 (ЕТ1) concentration was evaluated by immunoenzymatic method. We assessed other results of clinical and laboratory tests to compare them with morphological changes of the microcirculatory bed. Results. Most patients involved suffered from abnormal peripheral hemodynamics. It was revealed that kidneys, lungs or heart were damaged more frequently in the patients with peripheral blood circulation disorders, which were the most significant in the patients with SSc (p<0.05). Disorders of peripheral blood flow were exacerbated in case of lengthening of the disease course. Concentration of ЕТ1 was relevantly higher in the patients with peripheral blood flow disorders. Number of pathologic capillaries was the highest in the SSc patients. Conclusions. In terms of integral estimation, extremely significant changes of microcirculatory bloodstream were evidenced in the patients with SSc. However, some morphometric peculiarities were revealed in the patients without peripheral blood flow disorders. Thus, normal rheovasography did not exclude any microcirculation disorders.
{"title":"CONNECTIVE TISSUE DISEASES: FOCUS ON MICROCIRCULATORY BED","authors":"O. I. Zarudna, I. Venher, A. Dovbush","doi":"10.11603/ijmmr.2413-6077.2019.2.10671","DOIUrl":"https://doi.org/10.11603/ijmmr.2413-6077.2019.2.10671","url":null,"abstract":"Background. A microcirculatory bloodstream is a target, source and reason of the pathological process in patients with systemic connective tissue diseases. \u0000Objectives. This study is focused on meta-analyses of biopsy material of skin flaps harvested from patients’ fingers to identify specific morphological changes. \u0000Methods. A retrospective analysis of the medical records of 39 examinees with systemic sclerosis (SSc), 45 with Systemic Lupus Erythematosus (SLE), and 45 with rheumatoid arthritis (RA) was performed. The condition of peripheral hemodynamics was examined with longitudinal rheovasography of arms and legs. Endothelin-1 (ЕТ1) concentration was evaluated by immunoenzymatic method. We assessed other results of clinical and laboratory tests to compare them with morphological changes of the microcirculatory bed. \u0000Results. Most patients involved suffered from abnormal peripheral hemodynamics. It was revealed that kidneys, lungs or heart were damaged more frequently in the patients with peripheral blood circulation disorders, which were the most significant in the patients with SSc (p<0.05). Disorders of peripheral blood flow were exacerbated in case of lengthening of the disease course. Concentration of ЕТ1 was relevantly higher in the patients with peripheral blood flow disorders. Number of pathologic capillaries was the highest in the SSc patients. \u0000Conclusions. In terms of integral estimation, extremely significant changes of microcirculatory bloodstream were evidenced in the patients with SSc. However, some morphometric peculiarities were revealed in the patients without peripheral blood flow disorders. Thus, normal rheovasography did not exclude any microcirculation disorders.","PeriodicalId":14059,"journal":{"name":"International Journal of Medicine and Medical Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49237525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-12DOI: 10.11603/IJMMR.2413-6077.2019.1.10307
O. Shevchuk, I. M. Todor, N. Lukianova, N. Rodionova, V. Nikolaev, V. Chekhun
Background. Side effects of antineoplastic agents (especially leukopenia and neutropenia) could be the main limiting factors for efficient treatment. Objective. The research is aimed at the study of myeloprotective capability of biosimilars of granulocyte colony stimulating factor (G-CSF) and granular carbon oral adsorbent C2 in melphalan-induced bone marrow suppression in Guerin carcinoma-grafted rats. Methods. Melphalan at the dose of 5.5 mg/kg was used to promote bone marrow suppression in the Guerin carcinoma grafted rats. To fight myelosuppression, we used filgrastim and its analogue, designed and produced by IEPOR, a recombinant granulocyte colony-stimulating factor (r-GCSF). Carbon granulated enterosorbent C2 was used for enteral sorption therapy (bulk density γ=0.18 g/cm3, diameter of granules 0.15-0.25 mm, BET pore surface – 2162 m2/g). All rats were sacrificed on the 17th day after carcinoma cells inoculation or on the 8th day after Melphalan injection. Results. Alkylating cytostatic agent caused severe leukopenia (by 95.7%), neutropenia (by 73.9%), and thrombocytopenia (by 84.9%) in the experimental rats. Mortality rate was 57%. Filgrastim and enterosorption with carbon oral adsorbent C2 increased the studied indices, but the most prominent results were observed when combination of both factors was used. Studied means did not affect the anti-tumor efficacy of Melphalan alone and in combination. Conclusions. Our results are perspective for further investigation of the efficacy of the combination of carbon oral adsorbents and hematopoietic cytokines in cases of ameliorate anti-cancer chemotherapy side effects, and its implementation into clinics.
背景。抗肿瘤药物的副作用(特别是白细胞减少和中性粒细胞减少)可能是有效治疗的主要限制因素。目标。本研究旨在研究粒细胞集落刺激因子(G-CSF)生物仿制药和颗粒碳口服吸附剂C2对melphalin诱导的移植Guerin癌大鼠骨髓抑制的骨髓保护能力。方法。5.5 mg/kg剂量的美法兰可促进移植Guerin癌大鼠骨髓抑制。为了对抗骨髓抑制，我们使用了由重组粒细胞集落刺激因子(r-GCSF) IEPOR设计和生产的非格昔汀及其类似物。采用碳粒型肠吸收剂C2进行肠内吸收治疗(体积密度γ=0.18 g/cm3，颗粒直径0.15 ~ 0.25 mm, BET孔表面积- 2162 m2/g)。所有大鼠于癌细胞接种后第17天或注射美法兰后第8天处死。结果。烷基化细胞抑制剂引起大鼠严重白细胞减少(95.7%)、中性粒细胞减少(73.9%)和血小板减少(84.9%)。死亡率为57%。非格司提姆和碳口腔吸附剂C2的肠道吸附均能提高研究指标，但两者联合使用效果最显著。研究方法不影响美法兰单用和联用的抗肿瘤效果。结论。我们的研究结果为进一步研究碳口服吸附剂与造血细胞因子联合使用在改善抗癌化疗副作用的情况下的疗效，并将其应用于临床提供了前景。
{"title":"EFFICACY OF GRANULOCYTE COLONY-STIMULATING FACTOR AND ENTEROSORPTION IN MELPHALAN-INDUCED BONE MARROW SUPPRESSION IN GUERIN CARCINOMA GRAFTED RATS","authors":"O. Shevchuk, I. M. Todor, N. Lukianova, N. Rodionova, V. Nikolaev, V. Chekhun","doi":"10.11603/IJMMR.2413-6077.2019.1.10307","DOIUrl":"https://doi.org/10.11603/IJMMR.2413-6077.2019.1.10307","url":null,"abstract":"Background. Side effects of antineoplastic agents (especially leukopenia and neutropenia) could be the main limiting factors for efficient treatment. \u0000Objective. The research is aimed at the study of myeloprotective capability of biosimilars of granulocyte colony stimulating factor (G-CSF) and granular carbon oral adsorbent C2 in melphalan-induced bone marrow suppression in Guerin carcinoma-grafted rats. \u0000Methods. Melphalan at the dose of 5.5 mg/kg was used to promote bone marrow suppression in the Guerin carcinoma grafted rats. To fight myelosuppression, we used filgrastim and its analogue, designed and produced by IEPOR, a recombinant granulocyte colony-stimulating factor (r-GCSF). Carbon granulated enterosorbent C2 was used for enteral sorption therapy (bulk density γ=0.18 g/cm3, diameter of granules 0.15-0.25 mm, BET pore surface – 2162 m2/g). All rats were sacrificed on the 17th day after carcinoma cells inoculation or on the 8th day after Melphalan injection. \u0000Results. Alkylating cytostatic agent caused severe leukopenia (by 95.7%), neutropenia (by 73.9%), and thrombocytopenia (by 84.9%) in the experimental rats. Mortality rate was 57%. Filgrastim and enterosorption with carbon oral adsorbent C2 increased the studied indices, but the most prominent results were observed when combination of both factors was used. Studied means did not affect the anti-tumor efficacy of Melphalan alone and in combination. \u0000Conclusions. Our results are perspective for further investigation of the efficacy of the combination of carbon oral adsorbents and hematopoietic cytokines in cases of ameliorate anti-cancer chemotherapy side effects, and its implementation into clinics.","PeriodicalId":14059,"journal":{"name":"International Journal of Medicine and Medical Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46835629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-12DOI: 10.11603/IJMMR.2413-6077.2019.1.9897
E. I. Edibamode, K. Mordi, L. David, A. M. Eghoi
Background. External ear measurement is of utmost importance in reconstructive surgeries. Objectives. The present study is aimed at ascertaining sexual dimorphism in external ear anthropometry and ear lobe attachments among adults Ijaws in Bayelsa, Nigeria. Methods. A total of 112 adults within the age range of 18-50 yrs, who met the inclusion criteria, were involved in the study. Four linear dimensions of the ear, which are ear length (EL), ear width (EW), lobular length (LL) and lobular width (LW), were measured for both genders. The lobular attachment for both ears for males and females were also examined and results recorded. These data were subjected to Student t-test, Chi-square test, and Pearson’s correlation using SPSS version 20.0. Results. The mean values for EL, EW, LL, and LW for the left auricle in the males and females were 58.14±0.60, 27.41±0.37, 14.47±0.27, 13.50±0.34 and 57.90±0.16, 27.45±0.65, 15.41±0.31, 13.43±0.38 respectively. However, for the right auricle in the males and females, the values were 58.40±0.45, 28.21±0.68, 14.32±0.31, 13.04±0.32, and 56.66±1.10, 27.51±0.65, 15.58±0.29, 13.28±0.34 respectively. The left and right lobular length were the only parameters that proved statistical significance (p<0.05) in females compare to males. Pearson’s correlations between right and left sides for each of the parameters were positive and significant. Chi-square analysis revealed no significant relationship (p>0.05) between earlobe attachments and gender. Conclusions. Sexual dimorphism was thus established in the Ijaw population as regards lobular length dimensions. It is believed that the results of this study would be very useful for ear morphology and reconstructive surgeries.
{"title":"ANTHROPOMETRY OF THE EXTERNAL EAR AMONG ADULT IJAWS IN BAYELSA STATE OF NIGERIA","authors":"E. I. Edibamode, K. Mordi, L. David, A. M. Eghoi","doi":"10.11603/IJMMR.2413-6077.2019.1.9897","DOIUrl":"https://doi.org/10.11603/IJMMR.2413-6077.2019.1.9897","url":null,"abstract":"Background. External ear measurement is of utmost importance in reconstructive surgeries. \u0000Objectives. The present study is aimed at ascertaining sexual dimorphism in external ear anthropometry and ear lobe attachments among adults Ijaws in Bayelsa, Nigeria. \u0000Methods. A total of 112 adults within the age range of 18-50 yrs, who met the inclusion criteria, were involved in the study. Four linear dimensions of the ear, which are ear length (EL), ear width (EW), lobular length (LL) and lobular width (LW), were measured for both genders. The lobular attachment for both ears for males and females were also examined and results recorded. These data were subjected to Student t-test, Chi-square test, and Pearson’s correlation using SPSS version 20.0. \u0000Results. The mean values for EL, EW, LL, and LW for the left auricle in the males and females were 58.14±0.60, 27.41±0.37, 14.47±0.27, 13.50±0.34 and 57.90±0.16, 27.45±0.65, 15.41±0.31, 13.43±0.38 respectively. However, for the right auricle in the males and females, the values were 58.40±0.45, 28.21±0.68, 14.32±0.31, 13.04±0.32, and 56.66±1.10, 27.51±0.65, 15.58±0.29, 13.28±0.34 respectively. The left and right lobular length were the only parameters that proved statistical significance (p<0.05) in females compare to males. Pearson’s correlations between right and left sides for each of the parameters were positive and significant. Chi-square analysis revealed no significant relationship (p>0.05) between earlobe attachments and gender. \u0000Conclusions. Sexual dimorphism was thus established in the Ijaw population as regards lobular length dimensions. It is believed that the results of this study would be very useful for ear morphology and reconstructive surgeries.","PeriodicalId":14059,"journal":{"name":"International Journal of Medicine and Medical Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43059601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-12DOI: 10.11603/IJMMR.2413-6077.2019.1.10308
N. Volotovska, T. V. Kashchak
Background. Blood loss during civil and military limb trauma is the most common cause of preventable death. Complications due to the use of a hemostatic tourniquet are widely investigated nowadays. Therefore, the standards of the past have to be improved. Objective. The aim of the research is to study the reaction of the enzyme chain of the liver antioxidant system in the presence of modifications of ischemia-reperfusion injury (IRI). Methods. 210 white male-rats, aged 5-5.5 months, were used in the research. The dynamics of antioxidant enzymes activity catalase (Cat) and superoxide dismutase (SOD) in liver tissue in cases of modifications of ischemia-reperfusion injury (IRI) were studied. The period of investigation was in 24 hours, 3, 7, 14 days after the injury. Results. In cases of simulated IRI the catalase level mainly decreased at each period of the experiment. The peak of SOD activity was evidenced on the 1st, 3rd or 7th days after the experimental IRI according to the degree of trauma severity. Thus, IRI combined with severe blood loss and mechanical trauma caused the severest affection of the antioxidant system. Even a single application of hemostatic tourniquet caused similar wavelike reactions at different times. Conclusions. The development of IRI is accompanied by a significant depression of the liver antioxidant system. The most significant changes were evidenced in cases of IRI combined with blood loss and mechanical trauma, but even a single application of a tourniquet caused active response of the antioxidant enzymes.
{"title":"ANTIOXIDANT ENZYMES ACTIVITY IN EXPERIMENTAL ISCHEMIA-REPERFUSION INJURY","authors":"N. Volotovska, T. V. Kashchak","doi":"10.11603/IJMMR.2413-6077.2019.1.10308","DOIUrl":"https://doi.org/10.11603/IJMMR.2413-6077.2019.1.10308","url":null,"abstract":"Background. Blood loss during civil and military limb trauma is the most common cause of preventable death. Complications due to the use of a hemostatic tourniquet are widely investigated nowadays. Therefore, the standards of the past have to be improved. \u0000Objective. The aim of the research is to study the reaction of the enzyme chain of the liver antioxidant system in the presence of modifications of ischemia-reperfusion injury (IRI). \u0000Methods. 210 white male-rats, aged 5-5.5 months, were used in the research. The dynamics of antioxidant enzymes activity catalase (Cat) and superoxide dismutase (SOD) in liver tissue in cases of modifications of ischemia-reperfusion injury (IRI) were studied. The period of investigation was in 24 hours, 3, 7, 14 days after the injury. \u0000Results. In cases of simulated IRI the catalase level mainly decreased at each period of the experiment. The peak of SOD activity was evidenced on the 1st, 3rd or 7th days after the experimental IRI according to the degree of trauma severity. Thus, IRI combined with severe blood loss and mechanical trauma caused the severest affection of the antioxidant system. Even a single application of hemostatic tourniquet caused similar wavelike reactions at different times. \u0000Conclusions. The development of IRI is accompanied by a significant depression of the liver antioxidant system. The most significant changes were evidenced in cases of IRI combined with blood loss and mechanical trauma, but even a single application of a tourniquet caused active response of the antioxidant enzymes.","PeriodicalId":14059,"journal":{"name":"International Journal of Medicine and Medical Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48310253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-12DOI: 10.11603/IJMMR.2413-6077.2019.1.10306
V. Lukianchuk, T. Bukhtiarova, I. Seifullina, E. Polishchuk, O. E. Martsinko, H. A. Topolnytska
Background. Previous studies showed that new coordinate compound Cerebrogerm (ferrum bis(citrato)germanate) is a promising cerebroprotector. Objective of the study is a comparative analysis of the central stage of the pharmacokinetics of Cerebrogerm as well as its distribution in vital organs in cases of closed head injury. Methods. On the experimental original model of closed brain injury in rats the parameters of the central stage of Cerebrogerm pharmacokinetics: the distribution in brain, liver, kidneys, were studied. Results. It is established that, in cases of closed head injury Cerebrogerm reaches maximum concentration first in the brain (in 3.75 h), then in the kidneys (in 3.92 h), and finally in the liver (in 4.17 h). In this case, the magnitude of the Cmax of the coordinating compound of germanium that is being investigated in different biosubstrates of the rats with closed head injury may be presented in descending order as follows: brain (7.95 mg/L) > liver (6.22 mg/L) > kidneys (1.79 mg/L). Conclusions. The compound Cerebrogerm studied easily gets through the blood-brain barrier and meets the present requirements for cerebroprotectors and antihypoxants. The attained results allow noting that in the early post-traumatic period of closed head injury, the blood circulation in the kidneys does not change and cannot modify the absorption-elimination processes of xenobiotics. It has been also established that Cerebrogerm is distributed faster in the examined organs in cases of closed head injury. The highest concentration of the drug is present in the brain and the smallest one – in the kidneys.
{"title":"PHARMACOKINETICS OF THE NEW CEREBROPETECTOR FERRUM BIS(CITRATO)GERMANATE AT THE STAGE OF ITS DISTRIBUTION TO THE ORGANS IN CLOSED HEAD INJURY","authors":"V. Lukianchuk, T. Bukhtiarova, I. Seifullina, E. Polishchuk, O. E. Martsinko, H. A. Topolnytska","doi":"10.11603/IJMMR.2413-6077.2019.1.10306","DOIUrl":"https://doi.org/10.11603/IJMMR.2413-6077.2019.1.10306","url":null,"abstract":"Background. Previous studies showed that new coordinate compound Cerebrogerm (ferrum bis(citrato)germanate) is a promising cerebroprotector. \u0000Objective of the study is a comparative analysis of the central stage of the pharmacokinetics of Cerebrogerm as well as its distribution in vital organs in cases of closed head injury. \u0000Methods. On the experimental original model of closed brain injury in rats the parameters of the central stage of Cerebrogerm pharmacokinetics: the distribution in brain, liver, kidneys, were studied. \u0000Results. It is established that, in cases of closed head injury Cerebrogerm reaches maximum concentration first in the brain (in 3.75 h), then in the kidneys (in 3.92 h), and finally in the liver (in 4.17 h). In this case, the magnitude of the Cmax of the coordinating compound of germanium that is being investigated in different biosubstrates of the rats with closed head injury may be presented in descending order as follows: brain (7.95 mg/L) > liver (6.22 mg/L) > kidneys (1.79 mg/L). \u0000Conclusions. The compound Cerebrogerm studied easily gets through the blood-brain barrier and meets the present requirements for cerebroprotectors and antihypoxants. The attained results allow noting that in the early post-traumatic period of closed head injury, the blood circulation in the kidneys does not change and cannot modify the absorption-elimination processes of xenobiotics. It has been also established that Cerebrogerm is distributed faster in the examined organs in cases of closed head injury. The highest concentration of the drug is present in the brain and the smallest one – in the kidneys.","PeriodicalId":14059,"journal":{"name":"International Journal of Medicine and Medical Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42580969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-11DOI: 10.11603/IJMMR.2413-6077.2019.1.9857
N. Matkovska
Background. Alcohol is considered to be the main risk factor for adverse event deaths around the world. In Ukraine, mortality due to alcoholic liver disease (ALD) has taken the second place in the structure of death causes from diseases of the digestive system. Objective. The aim of the research was to study the peculiarities of the causes of death in the patients with alcoholic liver disease at the stage of liver cirrhosis (LC) based on the analysis of protocols of pathoanatomical research. Methods. The analysis of 149 protocols of the pathoanatomical study of the patients, who died from alcoholic LC, has been carried out at the premises of the Pathoanatomical Department of the Ivano-Frankivsk Regional Clinical Hospital in 2006-2018. Results. Most people were young and middle aged. Fatal cases were caused by decompensation of the LC with the development of hepatic, hepatic-renal, cardio-pulmonary insufficiency, pancreatic necrosis, gastrointestinal bleeding (GIB), sepsis, hepatocellular carcinoma (HCC). In 37.6 % of the patients the concomitant illness was coronary heart disease (CHD), 10.7 % of the people had hypertension. In 6 % of the patients, ischemic stroke of the brain was diagnosed. In most people atherosclerotic vascular changes were revealed. Conclusions. Excessive consumption of alcohol and, consequently, the development of LC, can be considered as an adverse factor in the reducing social standard of living. In the majority of people, who died from the decompensation of alcoholic LC, atherosclerotic vascular lesions have been detected. This indicates a significant prevalence of lipid metabolism disturbance in the people with alcoholic LC.
{"title":"MORTALITY ANALYSIS OF THE PATIENTS WITH ALCOHOLIC LIVER CIRRHOSIS","authors":"N. Matkovska","doi":"10.11603/IJMMR.2413-6077.2019.1.9857","DOIUrl":"https://doi.org/10.11603/IJMMR.2413-6077.2019.1.9857","url":null,"abstract":"Background. Alcohol is considered to be the main risk factor for adverse event deaths around the world. In Ukraine, mortality due to alcoholic liver disease (ALD) has taken the second place in the structure of death causes from diseases of the digestive system. \u0000Objective. The aim of the research was to study the peculiarities of the causes of death in the patients with alcoholic liver disease at the stage of liver cirrhosis (LC) based on the analysis of protocols of pathoanatomical research. \u0000Methods. The analysis of 149 protocols of the pathoanatomical study of the patients, who died from alcoholic LC, has been carried out at the premises of the Pathoanatomical Department of the Ivano-Frankivsk Regional Clinical Hospital in 2006-2018. \u0000Results. Most people were young and middle aged. Fatal cases were caused by decompensation of the LC with the development of hepatic, hepatic-renal, cardio-pulmonary insufficiency, pancreatic necrosis, gastrointestinal bleeding (GIB), sepsis, hepatocellular carcinoma (HCC). In 37.6 % of the patients the concomitant illness was coronary heart disease (CHD), 10.7 % of the people had hypertension. In 6 % of the patients, ischemic stroke of the brain was diagnosed. In most people atherosclerotic vascular changes were revealed. \u0000Conclusions. Excessive consumption of alcohol and, consequently, the development of LC, can be considered as an adverse factor in the reducing social standard of living. In the majority of people, who died from the decompensation of alcoholic LC, atherosclerotic vascular lesions have been detected. This indicates a significant prevalence of lipid metabolism disturbance in the people with alcoholic LC.","PeriodicalId":14059,"journal":{"name":"International Journal of Medicine and Medical Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45187672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-11DOI: 10.11603/IJMMR.2413-6077.2019.1.9737
B. Gerashchenko, K. Salmina, J. Eglītis, J. Erenpreisa
Background. Discrepancies in the interpretation of breast cancer therapeutic responses still exist mainly because of lack of standardized assessment criteria and methods. Objective. DNA content profiling of cells in the affected (cancerous) tissue before and after neoadjuvant chemotherapy (NAC) was applied to facilitate interpretation of therapeutic responses. Methods. Both diagnostic biopsy and operation materials representing the tissue of primary tumors surgically removed after NAC were subjected to DNA image cytometry. Polyploidy and aneuploidy in DNA histograms were evaluated with a prognostic Auer typing. Stemline DNA index (DI) values and percentages of cells that polyploidize (>4.5C) were also determined. Immunofluorescence staining was applied to evaluate proliferation (Ki-67), invasiveness (CD44), and self-renewal factors characteristic for stem cells (SOX2 and NANOG). Results. DNA content profiles of 12 breast cancer cases, of which 7 were triple-negative, revealed the features of tumor non-responsiveness to NAC in 7 cases, of which 5 were triple-negative. Among non-responsive cases there were 3 cases that showed enhanced polyploidization, suggesting the negative NAC effect. Near-triploid (DI=1.26-1.74) triple-negative cases were determined as most resistant to NAC. Cycling near-triploid cells may contribute to the excessive numbers of >4.5C cells. Polyploid cells were positive for Ki-67, CD44, SOX2, and NANOG. Conclusions. DNA content profiling data provide additional helpful information for interpreting therapeutic responses in NAC-treated breast cancers. Polyploid tumor cells possessing stem cell features can be induced by NAC. Because NAC effects in some cases may be unfavorable, the use of the further treatment strategy should be carefully considered.
{"title":"PROBING BREAST CANCER THERAPEUTIC RESPONSES BY DNA CONTENT PROFILING","authors":"B. Gerashchenko, K. Salmina, J. Eglītis, J. Erenpreisa","doi":"10.11603/IJMMR.2413-6077.2019.1.9737","DOIUrl":"https://doi.org/10.11603/IJMMR.2413-6077.2019.1.9737","url":null,"abstract":"Background. Discrepancies in the interpretation of breast cancer therapeutic responses still exist mainly because of lack of standardized assessment criteria and methods. \u0000Objective. DNA content profiling of cells in the affected (cancerous) tissue before and after neoadjuvant chemotherapy (NAC) was applied to facilitate interpretation of therapeutic responses. \u0000Methods. Both diagnostic biopsy and operation materials representing the tissue of primary tumors surgically removed after NAC were subjected to DNA image cytometry. Polyploidy and aneuploidy in DNA histograms were evaluated with a prognostic Auer typing. Stemline DNA index (DI) values and percentages of cells that polyploidize (>4.5C) were also determined. Immunofluorescence staining was applied to evaluate proliferation (Ki-67), invasiveness (CD44), and self-renewal factors characteristic for stem cells (SOX2 and NANOG). \u0000Results. DNA content profiles of 12 breast cancer cases, of which 7 were triple-negative, revealed the features of tumor non-responsiveness to NAC in 7 cases, of which 5 were triple-negative. Among non-responsive cases there were 3 cases that showed enhanced polyploidization, suggesting the negative NAC effect. Near-triploid (DI=1.26-1.74) triple-negative cases were determined as most resistant to NAC. Cycling near-triploid cells may contribute to the excessive numbers of >4.5C cells. Polyploid cells were positive for Ki-67, CD44, SOX2, and NANOG. \u0000Conclusions. DNA content profiling data provide additional helpful information for interpreting therapeutic responses in NAC-treated breast cancers. Polyploid tumor cells possessing stem cell features can be induced by NAC. Because NAC effects in some cases may be unfavorable, the use of the further treatment strategy should be carefully considered.","PeriodicalId":14059,"journal":{"name":"International Journal of Medicine and Medical Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47593724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：