D. M. Adcock, G. W. Moore, G. W. Kershaw, S. A. L. Montalvao, R. C. Gosselin
This guidance document has been prepared on behalf of the International Council for Standardization in Haematology (ICSH). The aim of the document is to provide guidance and recommendations for the performance and interpretation of activated partial thromboplastin time (APTT) and prothrombin time (PT) plasma mixing tests in clinical laboratories in all regions of the world. The following areas are included in this document: preanalytical, analytical, postanalytical, and quality assurance considerations as they relate to the proper performance and interpretation of plasma mixing tests. The recommendations are based on good laboratory practice, published data in peer-reviewed literature, and expert opinion.
{"title":"International Council for Standardization in Haematology (ICSH) recommendations for the performance and interpretation of activated partial thromboplastin time and prothrombin time mixing tests","authors":"D. M. Adcock, G. W. Moore, G. W. Kershaw, S. A. L. Montalvao, R. C. Gosselin","doi":"10.1111/ijlh.14344","DOIUrl":"10.1111/ijlh.14344","url":null,"abstract":"<p>This guidance document has been prepared on behalf of the International Council for Standardization in Haematology (ICSH). The aim of the document is to provide guidance and recommendations for the performance and interpretation of activated partial thromboplastin time (APTT) and prothrombin time (PT) plasma mixing tests in clinical laboratories in all regions of the world. The following areas are included in this document: preanalytical, analytical, postanalytical, and quality assurance considerations as they relate to the proper performance and interpretation of plasma mixing tests. The recommendations are based on good laboratory practice, published data in peer-reviewed literature, and expert opinion.</p>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"46 5","pages":"777-788"},"PeriodicalIF":2.2,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ijlh.14344","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141621996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
<p>Leukocytosis, defined as an increase in the number of white blood cells (WBC), is a common feature in hospitalized patients. The most common form of leukocytosis is the increase of neutrophilic granulocytes. A profound increase in neutrophilic granulocytes is also known as a leukemoid reaction (LR). Since the first introduction of the term “leukemoid reaction” by Krumbhaar in 1926,<span><sup>1</sup></span> its diagnosis has significantly improved when various laboratory methods were introduced to differentiate LR from malignant granulocytic proliferation. The prognosis for patients with LR relies primarily on their underlying causes; however, the mortality rate remains relatively high.<span><sup>2</sup></span> Therefore, a simple and reproducible method for the initial evaluation of blood, especially when ancillary studies are unavailable, is clinically paramount for improving patient care.</p><p>Different cutoffs have been reported in the literature for defining neutrophilic LR. Some authors have used a cutoff of 25.0 × 10<sup>9</sup> leukocytes/L<span><sup>3</sup></span>, while others have applied higher cutoffs of 40.0 × 10<sup>9</sup>/L, or 50.0 × 10<sup>9</sup>/L.<span><sup>4, 5</sup></span> Regardless of the cutoff, neutrophilic LR shares similar features with profound increases in neutrophils and precursors mimicking chronic leukemia of granulocytic lineage. The increase in immature forms in LR, also known as left-shift, however, is predominantly late forms (segmented neutrophils, band neutrophils) with a minority of intermediate forms (metamyelocytes, myelocytes) and no increase in early forms (promyelocytes, blasts). In addition, neutrophilic LR is usually associated with morphologic features of “toxic changes,” including heavy cytoplasmic granules (toxic granules), Dohle bodies, and cytoplasmic vacuoles. Clinical assessment of patients with potential LR relies heavily on these morphologic features.</p><p>One of the most important differential diagnoses of LR is chronic myeloid leukemia (CML), which may show similar findings when evaluating blood smears. CML is a stem cell neoplasm affecting all three lineages of hematopoietic cells, with the most profound proliferation in the granulocytic lineage. The patients typically present with marked granulocytosis with a predominance of neutrophilic granulocytes and precursors. Eosinophilic and basophilic lineages are also increased but to a lesser degree. CML can be differentiated from LR by several features when evaluating blood smears. CML shows a more prominent left-shift in neutrophilic granulocytes with the entire spectrum of immature forms. Eosinophils and basophils are also increased in CML. The granulocytic cells in CML typically lack the “toxic changes” commonly seen in LR.</p><p>The vast majority of CML and LR can be readily differentiated by a review of blood smears and clinical history. Occasionally, differentiating LR from CML can be challenging on blood smear review due to morph
使用 Microsoft Excel 整理数据，对范围、中位数、均值进行描述性分析，并使用 t 检验进行统计分析。P 值等于或低于 0.01 即为具有统计学意义。CML 患者的年龄从 21 岁到 83 岁不等，中位年龄为 56.5 岁。男女比例为 2.1:1。所有慢性骨髓性白血病患者均在慢性期表现为慢性骨髓性白血病。LR患者的年龄从18岁到90岁不等，中位年龄为61岁。男女比例为 1:1.4。LR的病因是感染（45%）、炎症（43%）和药物（18%）。CML 白细胞计数中位数为 86.75 × 109/L（31.0 × 109-365.1 × 109），而 LR 白细胞计数中位数为 33.5 × 109/L（25.6 × 109- 67.4 × 109）。对 CML 和 LR 病例进行的人工鉴别计数显示，CML 病例的偏骨髓细胞、髓细胞、原髓细胞、囊泡、嗜酸性粒细胞和嗜碱性粒细胞的百分比明显更高。然而，LR 中性粒细胞的百分比明显更高。为了评估白细胞计数在区分 LR 和慢性粒细胞性白血病方面的诊断性能，我们进行了接收者操作特征（ROC）曲线分析。ROC曲线下面积（AUC）为0.92（95% CI：0.85-0.99），显示出良好的鉴别能力。最佳临界值为 45 × 109/L，灵敏度为 0.93，特异度为 0.76，可确保识别出大多数 CML 病例。建议临床使用 45 × 109/L 的临界值来区分 LR 和 CML。然而，由于存在假阴性和假阳性结果，该临界值不能单独使用，应与所有可用的实验室结果结合使用，以进行最准确的评估。这些结果证实，CML 中的粒细胞增多倾向于更深层次的左移，中期和早期粒细胞增多。同时，LR 主要影响晚期患者，分段中性粒细胞增多。此外，在 CML 中更容易看到血泡，而在与感染和炎症相关的 LR 中始终检测不到血泡。CML 和 LR 的带状中性粒细胞计数无明显差异。正如预期的那样，嗜酸性粒细胞和嗜碱性粒细胞在 CML 中作为肿瘤群体的一部分而增加，而细菌感染和慢性疾病相关炎症通常不会影响嗜酸性粒细胞和嗜碱性粒细胞。可以预见的是，单核细胞通常不受这两种实体的影响，而且 CML 和 LR 的单核细胞计数没有明显差异。这项研究为文献提供了 CML 和 LR 中血细胞类别的数值范围和统计数据，可作为执业病理学家的有用指南，也可纳入医学教育的教科书中。该研究的局限性在于样本量较小，且主要是来自一家机构的住院患者，存在选择偏差。未来可能需要进行更大规模、多机构的研究来证实我们的发现。
{"title":"Comparison of blood cell counts in leukemoid reaction and chronic myeloid leukemia: A study using Scopio blood cell counter with statistical analysis","authors":"Alaa S. Hrizat, Jerald Z. Gong","doi":"10.1111/ijlh.14341","DOIUrl":"10.1111/ijlh.14341","url":null,"abstract":"<p>Leukocytosis, defined as an increase in the number of white blood cells (WBC), is a common feature in hospitalized patients. The most common form of leukocytosis is the increase of neutrophilic granulocytes. A profound increase in neutrophilic granulocytes is also known as a leukemoid reaction (LR). Since the first introduction of the term “leukemoid reaction” by Krumbhaar in 1926,<span><sup>1</sup></span> its diagnosis has significantly improved when various laboratory methods were introduced to differentiate LR from malignant granulocytic proliferation. The prognosis for patients with LR relies primarily on their underlying causes; however, the mortality rate remains relatively high.<span><sup>2</sup></span> Therefore, a simple and reproducible method for the initial evaluation of blood, especially when ancillary studies are unavailable, is clinically paramount for improving patient care.</p><p>Different cutoffs have been reported in the literature for defining neutrophilic LR. Some authors have used a cutoff of 25.0 × 10<sup>9</sup> leukocytes/L<span><sup>3</sup></span>, while others have applied higher cutoffs of 40.0 × 10<sup>9</sup>/L, or 50.0 × 10<sup>9</sup>/L.<span><sup>4, 5</sup></span> Regardless of the cutoff, neutrophilic LR shares similar features with profound increases in neutrophils and precursors mimicking chronic leukemia of granulocytic lineage. The increase in immature forms in LR, also known as left-shift, however, is predominantly late forms (segmented neutrophils, band neutrophils) with a minority of intermediate forms (metamyelocytes, myelocytes) and no increase in early forms (promyelocytes, blasts). In addition, neutrophilic LR is usually associated with morphologic features of “toxic changes,” including heavy cytoplasmic granules (toxic granules), Dohle bodies, and cytoplasmic vacuoles. Clinical assessment of patients with potential LR relies heavily on these morphologic features.</p><p>One of the most important differential diagnoses of LR is chronic myeloid leukemia (CML), which may show similar findings when evaluating blood smears. CML is a stem cell neoplasm affecting all three lineages of hematopoietic cells, with the most profound proliferation in the granulocytic lineage. The patients typically present with marked granulocytosis with a predominance of neutrophilic granulocytes and precursors. Eosinophilic and basophilic lineages are also increased but to a lesser degree. CML can be differentiated from LR by several features when evaluating blood smears. CML shows a more prominent left-shift in neutrophilic granulocytes with the entire spectrum of immature forms. Eosinophils and basophils are also increased in CML. The granulocytic cells in CML typically lack the “toxic changes” commonly seen in LR.</p><p>The vast majority of CML and LR can be readily differentiated by a review of blood smears and clinical history. Occasionally, differentiating LR from CML can be challenging on blood smear review due to morph","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"46 6","pages":"1125-1127"},"PeriodicalIF":2.2,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ijlh.14341","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141617796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Steven Bruzek, Marisol Betensky, Anthony A. Sochet, Neil A. Goldenberg, Vera Ignjatovic
Introduction
Fibrinolysis is a critical aspect of the hemostatic system, with assessment of fibrinolytic potential being critical to predict bleeding and clotting risk. We describe the method for a novel low-plasma-volume assay of fibrinolytic capacity utilizing the euglobulin fraction (the “modified mini-euglobulin clot lysis assay [ECLA]”), its analytic sensitivity to alterations in key fibrinolytic substrates/regulators, and its initial applications in acute and convalescent disease cohorts.
Methods
The modified mini-ECLA requires 50 μL of plasma, a maximal read time of 3 h (with most results available within 60 min), and is entirely performed in a 96-well microplate. Assay measurements were obtained in a variety of commercial control and deficient plasmas representing clinically relevant hypo- and hyperfibrinolytic states, and in three distinct adolescent cohorts with acute or convalescent illness: critically ill, following endotracheal intubation; acute COVID-19-related illness; and ambulatory, 3 months following a venous thromboembolic event.
Results
In 100% and 75% deficient plasmas, hypofibrinolysis for plasminogen-deficient, fibrinolysis for alpha-2-antiplasmin-deficient, and hyperfibrinolysis for plasminogen activator inhibitor-1-deficient plasmas were observed.
Conclusion
The modified mini-ECLA Clot Lysis Time Ratio (“CLTR”) demonstrated moderate-strength correlations with the Clot Formation and Lysis (CloFAL) assay, is analytically sensitive to altered fibrinolytic states in vitro, and correlates with clinical outcomes in preliminarily-studied patient populations.
{"title":"Methods, precision, and analytical sensitivity of a novel low-plasma-volume assay of fibrinolytic capacity utilizing the euglobulin fraction","authors":"Steven Bruzek, Marisol Betensky, Anthony A. Sochet, Neil A. Goldenberg, Vera Ignjatovic","doi":"10.1111/ijlh.14340","DOIUrl":"10.1111/ijlh.14340","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Fibrinolysis is a critical aspect of the hemostatic system, with assessment of fibrinolytic potential being critical to predict bleeding and clotting risk. We describe the method for a novel low-plasma-volume assay of fibrinolytic capacity utilizing the euglobulin fraction (the “modified mini-euglobulin clot lysis assay [ECLA]”), its analytic sensitivity to alterations in key fibrinolytic substrates/regulators, and its initial applications in acute and convalescent disease cohorts.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The modified mini-ECLA requires 50 μL of plasma, a maximal read time of 3 h (with most results available within 60 min), and is entirely performed in a 96-well microplate. Assay measurements were obtained in a variety of commercial control and deficient plasmas representing clinically relevant hypo- and hyperfibrinolytic states, and in three distinct adolescent cohorts with acute or convalescent illness: critically ill, following endotracheal intubation; acute COVID-19-related illness; and ambulatory, 3 months following a venous thromboembolic event.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>In 100% and 75% deficient plasmas, hypofibrinolysis for plasminogen-deficient, fibrinolysis for alpha-2-antiplasmin-deficient, and hyperfibrinolysis for plasminogen activator inhibitor-1-deficient plasmas were observed.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The modified mini-ECLA Clot Lysis Time Ratio (“CLTR”) demonstrated moderate-strength correlations with the Clot Formation and Lysis (CloFAL) assay, is analytically sensitive to altered fibrinolytic states in vitro, and correlates with clinical outcomes in preliminarily-studied patient populations.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"46 6","pages":"1092-1100"},"PeriodicalIF":2.2,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141565420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Didier Jambou, Noemie Saut, Viviane Queyrel, Anny Appert-Flory, Florence Fischer, Pierre Suchon, Neila De Pooter, Pierre Toulon
Introduction
G20210A (c.*97G>A) prothrombin gene variant, found in white population has been associated with an increased risk of venous thromboembolism (VTE). Other rare polymorphisms in F2 gene (C20209T) have been reported, more rare and touching black people, but its potential association with VTE remain uncertain.
Methods
About a 69 years-old Caucasian woman presenting an unprovoked deep venous thrombosis of the leg, we analyzed retrospectively 25.000 thrombophilia tests on a 11-year period of time (2007–2018), at Nice and Marseille University Hospitals, and performed extensive review of the literature.
Results
Genetic determination included a similar PCR protocol and sequencing. Twenty-one heterozygous cases out of 25.585 determinations (0.08%) was found. The C20209T mutation detected in our Caucasian patient is rare, with a frequency that differed from what was reported in the previous literature, mainly in non-Caucasian patients (Africans, Africans-Americans, and Caribbeans). One hundred and thirteen patients with this mutation have been described in the literature, of which only one homozygous.
Conclusion
This study is the most important on C20209T mutation performed at present, allowing to precise its frequency and its potential role in venous thromboembolism.
{"title":"F2c.*C20209T mutation in patients with a history of thrombosis: A case report, retrospective 2 site-results and review of the literature","authors":"Didier Jambou, Noemie Saut, Viviane Queyrel, Anny Appert-Flory, Florence Fischer, Pierre Suchon, Neila De Pooter, Pierre Toulon","doi":"10.1111/ijlh.14312","DOIUrl":"10.1111/ijlh.14312","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>G20210A (c.*97G>A) prothrombin gene variant, found in white population has been associated with an increased risk of venous thromboembolism (VTE). Other rare polymorphisms in F2 gene (C20209T) have been reported, more rare and touching black people, but its potential association with VTE remain uncertain.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>About a 69 years-old Caucasian woman presenting an unprovoked deep venous thrombosis of the leg, we analyzed retrospectively 25.000 thrombophilia tests on a 11-year period of time (2007–2018), at Nice and Marseille University Hospitals, and performed extensive review of the literature.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Genetic determination included a similar PCR protocol and sequencing. Twenty-one heterozygous cases out of 25.585 determinations (0.08%) was found. The C20209T mutation detected in our Caucasian patient is rare, with a frequency that differed from what was reported in the previous literature, mainly in non-Caucasian patients (Africans, Africans-Americans, and Caribbeans). One hundred and thirteen patients with this mutation have been described in the literature, of which only one homozygous.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study is the most important on C20209T mutation performed at present, allowing to precise its frequency and its potential role in venous thromboembolism.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"46 5","pages":"894-898"},"PeriodicalIF":2.2,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ijlh.14312","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141556256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Advantages of patient-based real-time quality control applications in modern quality assurance strategies","authors":"Tony Badrick, Jean-Marc Giannoli, Huub van Rossum","doi":"10.1111/ijlh.14338","DOIUrl":"10.1111/ijlh.14338","url":null,"abstract":"","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"46 6","pages":"1123-1124"},"PeriodicalIF":2.2,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141536220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Longrong Ran, Yu Peng, Mingyu Zhao, Xin Luo, Shuang Chen, Xinyi Tang, Yakun Zhang, Lian Li, Liangmei Li, Wei Zhang, Tingting Jiang, Xuelian Wu, Renzhi Hu, Yao Liu, Zailin Yang
<div> <section> <h3> Introduction</h3> <p>Autologous hematopoietic stem cell transplantation (ASCT) has gained extensive application in the treatment of lymphoma and multiple myeloma (MM). Plenty of studies demonstrate that peripheral blood indicators could be considered potential predictive biomarkers for hematopoietic stem cells (HSCs) collection efficiency, including white blood cell count (WBC), monocyte count (Mono), platelet count (PLT), hematocrit, and hemoglobin levels. Currently, clinically practical predictive models based on these peripheral detection indicators to quickly, conveniently, and accurately predict collection efficiency are lacking.</p> </section> <section> <h3> Methods</h3> <p>In total, 139 patients with MM and lymphoma undergoing mobilization and collection of ASCT were retrospectively studied. The study endpoint was successful collection of autologous HSCs. We analyzed the effects of clinical characteristics and peripheral blood markers on collection success, and screened variables to establish a prediction model. We determined the optimal cutoff value of peripheral blood markers for predicting successful stem cell collection and the clinical value of a multi-marker prediction approach. We also established a prediction model for collection efficacy.</p> </section> <section> <h3> Results</h3> <p>Univariate and multivariate logistic regression analyses showed that the mobilization regimen, Mono, PLT, mononuclear cell count (MNC), and peripheral blood CD34<sup>+</sup> cell count (PB CD34<sup>+</sup> counts) were significant predictors of successful collection of peripheral blood stem cells (PBSC). Two predictive models were constructed based on the results of multivariate logistic analyses. Model 1 included the mobilization regimen, Mono, PLT, and MNC, whereas Model 2 included the mobilization regimen, Mono, PLT, MNC, and PB CD34<sup>+</sup> counts. Receiver operating characteristic (ROC) curve analysis showed that the PB CD34<sup>+</sup> counts, Model 1, and Model 2 could predict successful HSCs collection, with cutoff values of 26.92 × 106/L, 0.548, and 0.355, respectively. Model 1 could predict successful HSCs collection with a sensitivity of 84.62%, specificity of 75.73%, and area under the curve (AUC) of 0.863. Model 2 could predict successful HSCs collection with a sensitivity of 83.52%, specificity of 94.17%, and AUC of 0.946; thus, it was superior to the PB CD34<sup>+</sup> counts alone.</p> </section> <section> <h3> Conclusion</h3> <p>Our findings suggest that the combination of the mobilization regimen, Mono, PLT, M
{"title":"Predictive model of the efficiency of hematopoietic stem cell collection in patients with multiple myeloma and lymphoma based on multiple peripheral blood markers","authors":"Longrong Ran, Yu Peng, Mingyu Zhao, Xin Luo, Shuang Chen, Xinyi Tang, Yakun Zhang, Lian Li, Liangmei Li, Wei Zhang, Tingting Jiang, Xuelian Wu, Renzhi Hu, Yao Liu, Zailin Yang","doi":"10.1111/ijlh.14337","DOIUrl":"10.1111/ijlh.14337","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Autologous hematopoietic stem cell transplantation (ASCT) has gained extensive application in the treatment of lymphoma and multiple myeloma (MM). Plenty of studies demonstrate that peripheral blood indicators could be considered potential predictive biomarkers for hematopoietic stem cells (HSCs) collection efficiency, including white blood cell count (WBC), monocyte count (Mono), platelet count (PLT), hematocrit, and hemoglobin levels. Currently, clinically practical predictive models based on these peripheral detection indicators to quickly, conveniently, and accurately predict collection efficiency are lacking.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>In total, 139 patients with MM and lymphoma undergoing mobilization and collection of ASCT were retrospectively studied. The study endpoint was successful collection of autologous HSCs. We analyzed the effects of clinical characteristics and peripheral blood markers on collection success, and screened variables to establish a prediction model. We determined the optimal cutoff value of peripheral blood markers for predicting successful stem cell collection and the clinical value of a multi-marker prediction approach. We also established a prediction model for collection efficacy.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Univariate and multivariate logistic regression analyses showed that the mobilization regimen, Mono, PLT, mononuclear cell count (MNC), and peripheral blood CD34<sup>+</sup> cell count (PB CD34<sup>+</sup> counts) were significant predictors of successful collection of peripheral blood stem cells (PBSC). Two predictive models were constructed based on the results of multivariate logistic analyses. Model 1 included the mobilization regimen, Mono, PLT, and MNC, whereas Model 2 included the mobilization regimen, Mono, PLT, MNC, and PB CD34<sup>+</sup> counts. Receiver operating characteristic (ROC) curve analysis showed that the PB CD34<sup>+</sup> counts, Model 1, and Model 2 could predict successful HSCs collection, with cutoff values of 26.92 × 106/L, 0.548, and 0.355, respectively. Model 1 could predict successful HSCs collection with a sensitivity of 84.62%, specificity of 75.73%, and area under the curve (AUC) of 0.863. Model 2 could predict successful HSCs collection with a sensitivity of 83.52%, specificity of 94.17%, and AUC of 0.946; thus, it was superior to the PB CD34<sup>+</sup> counts alone.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Our findings suggest that the combination of the mobilization regimen, Mono, PLT, M","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"46 6","pages":"1068-1076"},"PeriodicalIF":2.2,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ijlh.14337","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141499998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Leukocytes engulfing platelets: New evidence of thrombocytopenia in autoimmune necrotic myositis from morphological observation","authors":"Qiang Meng, Yang Fu","doi":"10.1111/ijlh.14336","DOIUrl":"10.1111/ijlh.14336","url":null,"abstract":"","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"47 1","pages":"5-7"},"PeriodicalIF":2.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141478254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yolanda Posada-Franco, Ana García-Álvarez, Elena Hernández-Álvarez, Irene Serrano-García, Rocío Contera-Raposo, Mercedes Martínez-Novillo González, María Teresa Sanz-Casla
Introduction
Reticulocyte count and novel derived parameters provide insight into the effectiveness of erythropoiesis and may be useful tools in the classification and diagnosis of anemias. However, there is no standardisation, so we consider it necessary that each laboratory evaluates the parameters according to its own methodology and instrumentation and establishes its own reference ranges. Our aim was to establish the reference intervals (RIs) of reticulocyte profile provided by the Beckman Coulter DxH 900 haematological autoanalyzer in our reference population.
Methods
One hundred and seventy-five healthy adults (18 to 62 years) were included. Subjects were collected from the blood donation centre of the Hospital Clínico San Carlos (Madrid, Spain) upon informed consent. Whole blood was collected and assayed for 14 haematological parameters on the Beckman Coulter DxH 900 analyzer in the haematology laboratory of the Clinical Analysis Department. RIs were established as per Clinical and Laboratory Standards Institute EP28-A3c guidelines using three different statistical approaches.
Results
RIs estimated using the non-parametric method and the Harrell-Davis bootstrap method were very similar. RIs estimated by the robust method were narrower. Gender partitioning was required for two haematological parameters (low haemoglobin density (LHD) and microcytic anaemia factor (MAF)). The rest of the parameters did not need to be partitioned according to Lahti's method.
Conclusion
RIs have been established for 14 hematologic parameters of the reticulocyte profile for the Beckman Coulter DxH 900 haematology analyzer using a healthy cohort of adult subjects.
{"title":"Reference intervals for reticulocyte count and derived reticulocyte parameters in a cohort of healthy adults","authors":"Yolanda Posada-Franco, Ana García-Álvarez, Elena Hernández-Álvarez, Irene Serrano-García, Rocío Contera-Raposo, Mercedes Martínez-Novillo González, María Teresa Sanz-Casla","doi":"10.1111/ijlh.14332","DOIUrl":"10.1111/ijlh.14332","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>Reticulocyte count and novel derived parameters provide insight into the effectiveness of erythropoiesis and may be useful tools in the classification and diagnosis of anemias. However, there is no standardisation, so we consider it necessary that each laboratory evaluates the parameters according to its own methodology and instrumentation and establishes its own reference ranges. Our aim was to establish the reference intervals (RIs) of reticulocyte profile provided by the Beckman Coulter DxH 900 haematological autoanalyzer in our reference population.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>One hundred and seventy-five healthy adults (18 to 62 years) were included. Subjects were collected from the blood donation centre of the Hospital Clínico San Carlos (Madrid, Spain) upon informed consent. Whole blood was collected and assayed for 14 haematological parameters on the Beckman Coulter DxH 900 analyzer in the haematology laboratory of the Clinical Analysis Department. RIs were established as per Clinical and Laboratory Standards Institute EP28-A3c guidelines using three different statistical approaches.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>RIs estimated using the non-parametric method and the Harrell-Davis bootstrap method were very similar. RIs estimated by the robust method were narrower. Gender partitioning was required for two haematological parameters (low haemoglobin density (LHD) and microcytic anaemia factor (MAF)). The rest of the parameters did not need to be partitioned according to Lahti's method.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>RIs have been established for 14 hematologic parameters of the reticulocyte profile for the Beckman Coulter DxH 900 haematology analyzer using a healthy cohort of adult subjects.</p>\u0000 </section>\u0000 </div>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"46 6","pages":"997-1003"},"PeriodicalIF":2.2,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141461417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bone marrow necrosis (BMN) is a clinically and pathologically poorly-defined and readily-overlooked entity. The current facts and guidelines pertaining to this entity are scarce, and there exist controversies. Upon reviewing the literature, we present the facts, analyze these controversies, and discourse on future prospects.
{"title":"Bone marrow necrosis: Facts, controversies, and perspective","authors":"Chengxin Luan, Hongguo Zhao, Yufei Ding","doi":"10.1111/ijlh.14335","DOIUrl":"10.1111/ijlh.14335","url":null,"abstract":"<p>Bone marrow necrosis (BMN) is a clinically and pathologically poorly-defined and readily-overlooked entity. The current facts and guidelines pertaining to this entity are scarce, and there exist controversies. Upon reviewing the literature, we present the facts, analyze these controversies, and discourse on future prospects.</p>","PeriodicalId":14120,"journal":{"name":"International Journal of Laboratory Hematology","volume":"46 5","pages":"770-776"},"PeriodicalIF":2.2,"publicationDate":"2024-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/ijlh.14335","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141461327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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