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Recombinant human BMP-2/-7 heterodimer protein expression for bone tissue engineering using recombinant baculovirus expression system 重组人BMP-2/-7异源二聚体蛋白在骨组织工程中的表达
Pub Date : 2016-06-30 DOI: 10.7852/IJIE.2016.32.2.49
Seung‐Won Park, T. Goo, Seong-Ryul Kim, Kwangho Choi
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引用次数: 0
The effects of Cudrania tricuspidata extract on bone metabolism in ovariectomized rats 三爪蟾提取物对去卵巢大鼠骨代谢的影响
Pub Date : 2016-06-30 DOI: 10.7852/IJIE.2016.32.2.80
You-Young Jo, S. Seo, Ji‐Won Kim, H. Cho, J. Chon, Kwang-gill Lee, H. Lee, Y. Park, H. Kweon
The effects of Cudrania tricuspidata (CT) extract on markers of osteoporosis were examined in ovariectomized rats. We classified 26 rats into five groups and provided a pellet chow diet and tap water throughout the 27-wk experimental period. During the last 15 wk, we added oral injections to each group as follows: sham-operated (SHAM, n=4) and ovariectomized-control (OVX, n=5) with distilled water, alendronate with 10 mg/kg/d of alendronate sodium (ALEN, n=5), CT (CT100, n=6) with 100 mg/kg/d of CT, and CT (CT300, n=6) with 300 mg/kg/d of CT. After the experimental period, blood, urine, and micro-CT images were assessed. The CT100 and OVX groups did not show any significant differences in urinary n-terminal telopeptide (NTx) ( p< 0.05), but with increases in CT concentration, the NTx level was slightly reduced. Serum osteocalcin was significantly higher in the CT groups than in all other groups ( p< 0.05). Notably, the serum calcium levels of all groups were within the normal range, but urinary calcium levels in the CT groups were significantly lower than the OVX group ( p< 0.05). In addition, the CT groups exhibited higher trabecular BMD than the OVX groups while showing similar BMD to the ALEN group ( p< 0.05). The Tb.Th of the ALEN group was lower than all other groups. Based on the overall analysis of results, CT prevented bone loss by inhibiting bone resorption and enhancing bone formation. Although alendronate showed a similar effect in preventing bone loss, it did so by solely inhibiting bone resorption, and its long-term use reportedly causes paradoxical effects such as hip fractures. Thus, for osteoporosis induced by ovariectomy, we conclude that CT extract is an effective natural treatment without severe side effects. Abstract
采用去卵巢大鼠,观察了三爪藤提取物对骨质疏松指标的影响。我们将26只大鼠分为5组,在27周的实验期间给予颗粒饲料和自来水。在最后15周,我们给每组分别口服注射:假手术(SHAM, n=4)和去卵巢对照(OVX, n=5)加蒸馏水,阿仑磷酸钠加阿仑磷酸钠10 mg/kg/d (ALEN, n=5), CT (CT100, n=6)加CT100 mg/kg/d, CT (CT300, n=6)加CT300 mg/kg/d。实验结束后,评估血液、尿液和显微ct图像。CT100组和OVX组尿n-末端末端肽(NTx)水平差异无统计学意义(p< 0.05),但随着CT浓度的升高,NTx水平略有降低。CT组血清骨钙素显著高于其他各组(p< 0.05)。各组血清钙水平均在正常范围内,但CT组尿钙水平显著低于OVX组(p< 0.05)。CT组骨小梁骨密度高于OVX组,与ALEN组相近(p< 0.05)。结核病。ALEN组的血压低于其他各组。综合分析结果,CT通过抑制骨吸收和促进骨形成来防止骨丢失。尽管阿仑膦酸钠在防止骨质流失方面显示出类似的效果,但它仅仅是通过抑制骨吸收来实现的,据报道,长期使用阿仑膦酸钠会产生矛盾的效果,如髋部骨折。因此,对于卵巢切除术所致的骨质疏松症,我们认为CT提取物是一种有效的天然治疗方法,而且没有严重的副作用。摘要
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引用次数: 0
Breeding of biparental sex-limited larval marking yellow cocoon variety "Hanbyeolnue" 双亲本性限幼虫标记黄茧品种“韩白茧”的选育
Pub Date : 2016-06-30 DOI: 10.7852/IJIE.2016.32.2.54
Kee-Young Kim, S. Seo, Mi-Ja Kim, S. Ji, Gyoo-Byung Sung, Yong‐Soon Kim, Wan-Taek Ju, Hae-yong Kwon, B. Sohn, Pil-Don Kang
All of silkworm varieties reared in farmhouses are first generation hybrids, for the production of these hybrid silkworms precise and labor-saving sex discrimination is necessary. The new variety “Hanbyeolnue” is biparental sex-limited larval marking yellow cocoon variety which was bred from biparental sex-limited strain of Japanese originated Jam 319 and Chinese originated Jam 320. Productivity test of Hanbyeolnue in 2015 showed high healthiness and short larval period. The Hanbyeolnue was evaluated as an excellent variety which can be utilized for special purpose silkworm such as male pupae or Cordyceps production. et al ., 2000) , Hansaengjam(Kang et al ., 2011) and Dodamnue, and sex-limited biparental yellow cocoon variety Hwangbojam(Kang et al ., 2013) were developed by NAAS, RDA and being supplied to farmhouses. To increase the sex discrimination efficiency, the biparental sex-limited larval marking yellow cocoon variety was developed to integrate advantage of each variety. The Hanbyeolnue designated as a recommended silkworm variety was bred from sex-limited larval marking yellow cocoon variety parent and it is superior to biparental sex-limited larval marking varieties and biparental sex-limited yellow cocoon varieties in practical sex discrimination efficiency. This variety can be utilized for producing functional material which can Abstract
家蚕都是第一代杂交品种,为了生产这些杂交品种的蚕,精确省力的性别区分是必要的。新品种“韩白糯”是由日本原产果酱319和中国原产果酱320的双亲本性限株系培育而成的双亲本性限幼虫标记黄茧品种。2015年的产力试验显示,汉别柳健康度高,幼虫期短。该品种被认为是一种优良品种,可用于生产特殊用途的蚕蛹或虫草。Hansaengjam(Kang et al ., 2011)和Dodamnue,以及性别限制双亲本黄茧品种Hwangbojam(Kang et al ., 2013)是由NAAS和RDA开发并供应给农户的。为提高黄茧的性别识别效率,综合各品种的优势,培育了双亲本性限幼虫标记黄茧品种。推荐品种韩白蚕以性限幼虫标记黄茧品种为亲本选育而成,在实际性别识别效率上优于双亲本性限幼虫标记品种和双亲本性限黄茧品种。该品种可用于生产可抽象的功能材料
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引用次数: 1
Effects of Brewer's spent grain (BSG) on larval growth of mealworms, Tenebrio molitor (Coleoptera: Tenebrionidae) Brewer's spent grain (BSG)对粉蚧幼虫生长的影响(鞘翅目:粉蚧科)
Pub Date : 2016-03-31 DOI: 10.7852/IJIE.2016.32.1.41
Sunyoung Kim, H. Kim, K. Lee, H. Yoon, N. Kim
Mealworms, Tenebrio molitor (Coleoptera: Tenebrionidae), are widely used as food source for animal rearing as well as human diet. Conventionally, mealworms raised on wheat bran. In this study, we investigated the effects of brewer’s spent grain (BSG) on the growth of mealworm larvae to reduce the rearing cost. We prepared five different diets with various BSG content, 0, 10, 30, 50, and 70% of heated air dried BSG with wheat bran. We compared survivorship, larval weight, developmental duration, pupation rate, and pupal weight of five different groups of T. molitor raised on these five diets. The larval survivorships were almost similar with the control group except a group with 70% of BSG. For the larval weight, groups with 30% and 50% of BSG gained more weight than that with control treatment, 0% of BSG. For the developmental period, the groups with 30% of BSG took less time compared to the control group. In all treatment groups, more than 90% of pupation rate were observed. Especially, the group with 30% of BSG showed highest pupation rate among the five groups. Wheat bran with 30~50% of BSG was the optimal diet for successive insect rearing among the five diet treatments. Based on this study, we concluded that adding BSG to wheat bran helps to improve the quality of T. molitor and to reduce the rearing period.
粉虫(鞘翅目:粉虫科)是一种广泛用于动物饲养和人类饮食的食物来源。通常,粉虫以麦麸为食。为降低粉虫的饲养成本,研究了啤酒废粮对粉虫幼虫生长的影响。我们准备了5种不同BSG含量的日粮,分别是0、10、30、50和70%的小麦麸皮热风干BSG。我们比较了在这五种饲料上饲养的五组褐飞蛾的存活率、幼虫体重、发育时间、化蛹率和蛹重。除BSG占70%外,其余各组幼虫成活率与对照组基本相同。在幼虫体重方面,添加30%和50%肉豆粕组的增重高于对照组(添加0%肉豆粕组)。在发育期,BSG含量为30%的组比对照组所用的时间更短。各处理组化蛹率均在90%以上。其中,BSG含量为30%组的化蛹率最高。在5种饲粮处理中,添加30~50%粗纤维素的麦麸是连续饲养昆虫的最佳饲粮。综上所述,在麦麸中添加BSG有助于提高小麦品质,缩短饲养周期。
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引用次数: 15
Safety evaluation of cricket(Gryllus bimaculatus) extract in Sprague-Dawley rats 蟋蟀提取物对大鼠的安全性评价
Pub Date : 2016-03-31 DOI: 10.7852/IJIE.2016.32.1.12
Somin Lee, K. Ahn, H. Ryu, H. Kim, Jinkyu Lee, M. Cho, M. Ahn, K. Song
Recently, research investment in the improvement of food safety as a food source and specializing of nutritional source of edible insects is being actively conducted. Cricket especially has been attracting considerable interest in entomophagy; however, research on the safety assessment of cricket is limited. This study investigated the effects of cricket ethanol extract when orally administrated in Sprague-Dawley rats. Here, we performed a 4 wk repeated oral dose toxicity test in Sprague-Dawley rats following the Organization for Economic Cooperation and Development test guidelines 407 under Good Laboratory Practice regulation. Rats were randomly allocated 4 groups: vehicle control, 250, 500, 1,000 mg/kg test groups and administrated based on body weight for 28 d. The animals were observed for mortalities and clinical signs, body weight changes, food and water consumption. At the end of treatment period, blood and urine were collected and analyzed. Subsequently, the animals were sacrificed and subjected to gross pathological examination and organ weight measurement. The organs were preserved for histopathological examination. The results showed that there were no systemic toxicological effects related with the cricket ethanol extract in the 4 wk oral repeated dose toxicity study. It is considered that NOAEL of cricket ethanol extract is greater than 1,000 mg/kg/d and there was no target organ detected.
近年来,作为食用昆虫食品来源和营养来源专业化的食品安全提升研究投入正在积极进行。蟋蟀尤其吸引了人们对食虫的极大兴趣;然而,对板球安全评价的研究却十分有限。本研究考察了蟋蟀乙醇提取物口服给药对Sprague-Dawley大鼠的影响。在这里,我们按照经济合作与发展组织良好实验室规范下的测试指南407,对Sprague-Dawley大鼠进行了为期4周的重复口服剂量毒性试验。将大鼠随机分为4组:载药对照组、250、500、1000 mg/kg试验组,按体重给药28 d,观察动物死亡率、临床体征、体重变化、食量和饮水量。在治疗期结束时,采集血液和尿液进行分析。随后处死动物,进行大体病理检查和脏器重量测定。器官保存以作组织病理学检查。结果表明,在4周的口服重复给药毒性研究中,蟋蟀乙醇提取物未出现与全身毒理学效应相关的作用。认为蟋蟀乙醇提取物的NOAEL大于1000 mg/kg/d,未检出靶器官。
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引用次数: 12
Influence of forestry host plants and rearing seasons on silk gland weight of tropical tasar silkworm, Antheraea mylitta (Lepidoptera: Saturniidae) under Doon valley conditions of Uttarakhand in India 印度北阿坎德邦Doon山谷条件下,林业寄主植物和饲养季节对热带绢蚕(Antheraea mylitta)丝腺重量的影响
Pub Date : 2016-03-31 DOI: 10.7852/IJIE.2016.32.1.1
N. K. Bhatia, M. Yousuf, P. Tewary, Satyasheel Sharma
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引用次数: 0
Potential use of essential oils to control the leaf-cutting ants; Atta sexdens rubropilosa and Acromyrmex subterraneus molestans (Hymenoptera: Formicidae) 精油控制切叶蚁的潜在用途;红茧小蜂及地下小蜂(膜翅目:蚁科)
Pub Date : 2016-03-31 DOI: 10.7852/IJIE.2016.32.1.26
R. Ribeiro, H. Fouad
The present study was developed in order to evaluate the effect of five essential oils on the workers of the leaf-cutting ants; Atta sexdens rubropilosa and Acromyrmex subterraneus molestans by contact with a treated surface and ingestion with a treated leaves.. The essential oils of cinnamon, clove and mustard had generally more effective with 5, 10 and 15% concentrations after 24, 48, 72 and 96 h against workers of A. sexdens rubropilosa and A. subterraneus molestans in contact bioassay, but mustard was the most effective in ingestion bioassay on both species. On the other hand, there was no significant difference among the essential oils with 1% concentration and control after 24, 48 and 72 h of treatment in contact and ingestion bioassays against workers of A. sexdens rubropilosa . However, Andiroba oil had less efficiency values in all concentrations been used. Therefore, the essential oils of mustard, cinnamon and clove have contact and ingestion effects on workers of A. sexdens rubropilosa and A. subterraneus molestans, and may be promising on the leaf-cutting ant control.
本研究旨在评价5种精油对切叶蚁工蚁的影响;通过接触处理过的表面和食用处理过的叶子,红毛螨和地底Acromyrmex肉桂精油、丁香精油和芥菜精油分别以5%、10%和15%的浓度处理24、48、72和96 h时,对红毛田鼠和地下田鼠的接触生物试验效果较好,而芥菜精油对这两种工蜂的食入生物试验效果最好。另一方面,处理24、48和72 h后,1%浓度精油与对照精油对红毛斑姬螨工蜂的接触和食入生物测定无显著差异。然而,在使用的所有浓度下,Andiroba油的效率值都较低。因此,芥菜精油、肉桂精油和丁香精油对红叶裂叶蚁和地下裂叶蚁的工蜂具有接触和摄食作用,在防治切叶蚁方面有一定的应用前景。
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引用次数: 1
Structural and thermal characteristics of photocrosslinked silk fibroin - PEG hydrogel 光交联丝素-聚乙二醇水凝胶的结构和热特性
Pub Date : 2016-03-31 DOI: 10.7852/IJIE.2016.32.1.35
You-Young Jo, Kwang-gill Lee, J. C. Bragg, Chien-Chi Lin, H. Kweon
Hydrogels are crosslinked hydrophilic matrices for a variety of biomedical applications. Silk fibroin (SF), one of typical natural biomaterials, has been explored as base material for hydrogel. Photocrosslinked SF hydrogel containing poly(ethylene glycol) (PEG) was formulated through visible light initiated thiol-acrylate photopolymerization. The morphological, structural and thermal properties of SF . PEG hydrogel was investigated through scanning electron microscopy, X-ray diffractometry, thermogravimetry, and differential scanning calorimetry. The morphology of SF hydrogel showed dot and uneven surface with network cross-section. X-ray diffraction curves showed that the specific diffraction peaks of PEG were not changed by the intensity of the peaks were affected by sonication. Thermo-degradation behavior of SF . PEG hydrogel sonicated was significantly affected and became complex pattern compared to unsonicated ones. However, the melting endothermic temperature of SF-PEG hydrogel was not changed but the crystalline enthalpy was decreased by gelation and sonication.
水凝胶是交联的亲水基质,用于各种生物医学应用。丝素蛋白是一种典型的天然生物材料,是制备水凝胶的基础材料。采用可见光引发巯基丙烯酸酯光聚合法制备了含聚乙二醇(PEG)的光交联SF水凝胶。SF的形态、结构和热性能。通过扫描电镜、x射线衍射、热重法和差示扫描量热法对PEG水凝胶进行了研究。SF水凝胶形貌呈圆点状,表面凹凸不平,截面呈网状。x射线衍射曲线表明,聚乙二醇的具体衍射峰不受声波强度的影响。SF的热降解行为。聚乙二醇水凝胶经超声处理后,与未经超声处理的水凝胶相比,受到明显的影响,形成了复杂的图案。经凝胶和超声处理后,SF-PEG水凝胶的熔融吸热温度没有变化,但结晶焓降低。
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引用次数: 0
Effect of temperature on the development of the Common Grass Yellow, Eurema hecabe 温度对普通草黄(Eurema hecabe)发育的影响
Pub Date : 2015-12-31 DOI: 10.7852/IJIE.2015.31.2.35
Seonghyun Kim, Haechul Park, I. Park
The developmental responses of insects to temperature are important considerations in gaining a better understanding of their ecology and life histories. Temperature-dependent phenology models permit examination of the effects of temperature on the geographical distributions, population dynamics, and management of insects. Measurements of insect developmental and survival responses to temperature pose practical challenges that depend on the chosen modality, variability among individuals, and high mortality rates near the lower and upper threshold temperatures. Different temperature levels can significantly affect larval development of Eurema hecabe. The development of E. hecabe reared on leaves of Lespedeza cuneata was investigated at three temperature regimes (20, 25, and 30°C), a relative humidity of 60%, and a light:dark photoperiod of 14:10 h. The developmental time from larva to adult was 34.3, 20.6, and 17.9 d at temperatures of 20, 25, and 30°C, respectively. Pupal rate was 47.6%, 47.6%, and 61.9% at temperatures of 20, 25, and 30°C, respectively. The developmental threshold temperature estimated from larva to pupae was 8.1°C with 381.7 degree-days. There is an increasing need for a standardized manual for rearing this butterfly species based on adequate knowledge of its ecology.
昆虫对温度的发育反应是更好地了解其生态和生活史的重要考虑因素。依赖温度的物候模型可以检验温度对地理分布、种群动态和昆虫管理的影响。测量昆虫对温度的发育和生存反应带来了实际的挑战,这取决于所选择的方式、个体之间的差异以及在下限和上限温度附近的高死亡率。不同的温度水平可以显著地影响小木鱼幼虫的发育。在20、25和30℃的相对湿度60%和14:10 h的光照条件下,研究了胡枝子叶片上饲养的小叶蝉的发育情况。在20、25和30℃的光照条件下,小叶蝉从幼虫到成虫的发育时间分别为34.3、20.6和17.9 d。在20℃、25℃和30℃时,蛹率分别为47.6%、47.6%和61.9%。从幼虫到蛹的发育阈温度为8.1℃,381.7℃。人们越来越需要一本标准化的手册,在充分了解其生态的基础上饲养这种蝴蝶。
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引用次数: 1
Positive affect of insect-mediated mental healthcare program to adolescent`s emotions 昆虫介导的心理保健项目对青少年情绪的正向影响
Pub Date : 2015-12-31 DOI: 10.7852/IJIE.2015.31.2.90
S. Bae, T. Shin, Young-Soon Jun, S. Woo
Several insects have been shown to exert a strong influence positively on peoples` emotions. Insect-mediated mental healthcare program was designed to help meet the physical, behavioral and developmental needs of adolescents. They were provided with an insectmediated mental healthcare program for a total of 10 sessions, one session per wk, at 1 h per session, accompanied by a pre- and post-test for emotion. Significant differences and correlations were found according to the individual participants` planning, subjective quality of life, self-reported depression and self-worth, respectively. The results indicated that adolescents who participated in the insect-mediated healthcare program group once showed significant improvement in their planning, subjective quality of life and self-worth. However, most of all participants` self-reported depression was not influenced. These findings suggest that pet-insects positively affect adolescent`s emotional health via an insect-mediated healthcare program.
有几种昆虫已被证明对人们的情绪产生强烈的积极影响。昆虫介导的心理保健项目旨在帮助满足青少年的身体、行为和发展需求。他们接受了一项昆虫介导的心理保健计划,共10次,每周一次,每次1小时,同时进行了情绪前和后测试。个体计划、主观生活质量、自我报告抑郁和自我价值感分别存在显著差异和相关。结果表明,参加过一次昆虫媒介保健方案组的青少年在计划、主观生活质量和自我价值感方面均有显著改善。然而,大多数参与者自我报告的抑郁并未受到影响。这些研究结果表明,宠物昆虫通过昆虫介导的医疗保健计划对青少年的情绪健康产生积极影响。
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引用次数: 1
期刊
International journal of industrial entomology
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