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Characterization of DNJ production for large-scale fermentation of mulberry leaf 桑叶大规模发酵生产DNJ的特性研究
Pub Date : 2017-01-01 DOI: 10.7852/IJIE.2017.35.2.111
O. Kwon, Wan-Taek Ju, Hyun-bok Kim, Gyoo-Byung Sung, Yong‐Soon Kim
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引用次数: 4
Comparison of silkworm powder from 3 Bombyx mori varieties on alcohol metabolism in rats 3个家蚕品种家蚕粉对大鼠酒精代谢的影响比较
Pub Date : 2017-01-01 DOI: 10.7852/ijie.2017.35.1.22
Da-Young Lee, Jae-Min Cho, S. Yun, K. Hong, S. Ji, Jong-Gon Son, Eun-Hee Kim
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引用次数: 1
Comparative effect of silkworm powder from 3 Bombyx mori varieties on ethanol-induced gastric injury in rat model 3个家蚕品种家蚕粉对乙醇性大鼠胃损伤模型的比较作用
Pub Date : 2017-01-01 DOI: 10.7852/IJIE.2017.35.1.14
Da-Young Lee, Jae-Min Cho, S. Yun, K. Hong, S. Ji, Jong-Gon Son, Eun-Hee Kim
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引用次数: 5
Effects of olfactory self- and cross-adaptation on perceiving odor in a moth 嗅觉自适应和交叉适应对飞蛾气味感知的影响
Pub Date : 2016-12-31 DOI: 10.7852/IJIE.2016.32.2.60
K. Qian, Haibin Chen, X. Wan
Pheromone orientation in moths is an exemplar of olfactory sensitivity. To avoid cross mating, the responses of males to pheromone blends must be high specificity and temporal resolution. We tested the effects of olfactory self- and cross-adaptation of pheromone compounds and mixtures in Spodoptera litura moths by electroantennogram (EAG) recordings. The challenge of S. litura antennae to a pulse train of its own pheromone blends of Z9,E11-14:OAc and Z9,E12-14:OAc with 200 ms on/off and 1 s on/off indicated that the repetitive stimulation by 200 ms on/off with high dosages resulted in greater adaptation than that by 1 s on/off with low dosages and the adaptation index of Z9,E11-14:OAc in all treatments is significantly larger than that of Z9,E12-14:OAc, suggesting that high dosages with more frequent stimulation prefer to induce sensory adaptations and a different odor coding exist between the two components in the antennal periphery in this moth. The cross-adaptation EAG test among the two pheromone compounds and Z7-12:OAc and Z9-14:OH from congeneric species of S. litura showed that each of these compounds adapted the antenna more to that specific compound. The significantly higher adaptation to Z7-12:OAc and Z9-14:OH than to the pheromone components of S. litura induced by themselves suggested that both of them are coded by specific odor receptor neurons which are different from those tuned to the pheromone components of S. litura . Thus, we proposed that Z7-12:OAc and Z9-14:OH may play an important role in avoidance of heterospecific mating between S. litura and its sympatric moth species.
蛾的信息素取向是嗅觉敏感性的一个例子。为了避免交叉交配,雄性对信息素混合物的反应必须具有高特异性和时间分辨率。通过触角电图(EAG)记录,研究了斜纹夜蛾嗅觉自适应和交叉适应对信息素化合物和混合物的影响。通过对其自身信息素混合物Z9、E11-14:OAc和Z9、E12-14:OAc在200 ms开/关和1 s开/关脉冲序列的挑战,表明高剂量200 ms开/关的重复刺激比低剂量1 s开/关的适应性更强,且Z9、E11-14:OAc在所有处理中的适应指数均显著大于Z9、E12-14:OAc。这表明高剂量和更频繁的刺激更倾向于诱导感觉适应,并且该蛾触角周围的两种成分之间存在不同的气味编码。两种信息素化合物与同属种斜纹山茱萸的Z7-12:OAc和Z9-14:OH的交叉适应性EAG测试表明,这两种信息素化合物对天线的适应性更强。对Z7-12:OAc和Z9-14:OH诱导的适应性明显高于对斜纹山茱萸信息素成分诱导的适应性,这表明它们都是由特定的气味受体神经元编码的,与对斜纹山茱萸信息素成分调节的神经元不同。因此,我们认为Z7-12:OAc和Z9-14:OH可能在斜纹夜蛾与同域飞蛾避免异种交配中起重要作用。
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引用次数: 0
Antioxidant activity of silkworm powder treated with protease 蛋白酶处理蚕粉抗氧化活性的研究
Pub Date : 2016-12-31 DOI: 10.7852/IJIE.2016.33.2.78
S. Bae, You-Young Jo, Kwang-gill Lee, Hyun-bok Kim, H. Kweon
The antioxidant activity of silkworm powder treated by proteolytic enzyme was investigated. Total protein content of silkworm power was assayed using BCA, Bradford assays and SDS-polyacrylamide gel electrophoresis (PAGE) with alkaline protease treatment conditions including temperature and pH. The optimum condition of alkaline protease treatment for silkworm powder was found to be 60℃ and pH 7. The alkaline protease treatment resulted in increased contents of free amino acids, total polyphenol and total flavonoid compared to control group. The silkworm hydrolysates showed excellent antioxidant activities in various in vitro models such as 2,2 diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, 2,2 - azino-bis(3-ethylbenzthiazoline-6)-sulfonic acid (ABTS) radical scavenging activity. These results provide useful information for using silkworm powder as an ingredient in functional foods and for exploiting alkaline protease treatment to improve the extractability and bioactivity of a raw material.
研究了蚕粉经蛋白水解酶处理后的抗氧化活性。采用BCA法、Bradford法和sds -聚丙烯酰胺凝胶电泳法(PAGE)测定了蚕粉的总蛋白含量,碱酶处理条件包括温度和pH,确定了碱性蛋白酶处理蚕粉的最佳条件为60℃、pH 7。碱性蛋白酶处理使游离氨基酸、总多酚和总黄酮含量高于对照组。家蚕水解产物对2,2 -二苯基-1-吡啶肼(DPPH)自由基的清除能力、2,2 -氮基-双(3-乙基苯并噻唑-6)-磺酸(ABTS)自由基的清除能力等体外实验均显示出良好的抗氧化活性。这些结果为蚕粉作为功能性食品的原料和利用碱性蛋白酶处理提高原料的可提取性和生物活性提供了有益的信息。
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引用次数: 2
Effect of degumming on structure and mechanical properties of silk textile made with silk/polyurethane core-spun yarn 脱胶对丝/聚氨酯包芯纱真丝织物结构和力学性能的影响
Pub Date : 2016-12-31 DOI: 10.7852/IJIE.2016.33.2.132
Y. Bae, Chunho Kim, D. Bae, I. Um
Although silk textile shows excellent performance when used in clothing over a long period, its limited elongation and elasticity have restricted its extension to other textile and non-textile applications. In the present study, silk textile was produced using silk/ polyurethane core-spun yarn and degummed to enhance its elongation and elasticity. The effects of degumming on the structure and mechanical properties of the silk textile were examined. Scanning electron microscopy observation revealed that the silk filaments became finer and more flexible with degumming, resulting in increased tangling of weft yarns and a highly shrunk textile structure in the weft direction. Although the strength of the degummed silk textile was decreased, its elongation greatly increased by 383% (a 16-fold increase) because of the degumming treatment. In particular, the elasticity of the silk textile was greatly improved. The silk textile exhibited ~30% reduction in the elongation after the second extension; however, the elongation almost did not change after 18 additional extension–recovery tests. its use in other textile and non-textile applications. In a previous study, as a preliminary work, silk textile was made with silk/ polyurethane core-spun yarn and treated with hot water to improve its elongation and elasticity and Um, 2016). However, the results were unpromising, with the silk textile exhibiting 96.7% elasticity, even although it was just extended by an elongation of 20%. In the present study, the silk textile made with silk/ polyurethane core-spun yarn was degummed to enhance its elasticity, and the effects of degumming on the structure and mechanical properties of the silk textile were examined.
虽然丝绸在长期的服装使用中表现出优异的性能,但其有限的伸长率和弹性限制了其在其他纺织和非纺织领域的应用。本研究以真丝/聚氨酯包芯纱为原料,通过脱胶提高其伸长率和弹性来生产真丝纺织品。研究了脱胶对真丝织物结构和力学性能的影响。扫描电镜观察发现,脱胶后丝丝变得更细、更柔韧,导致纬纱缠结增加,织物结构在纬向高度收缩。脱胶后的丝织物虽然强度下降,但伸长率却大幅度提高了383%(提高了16倍)。特别是真丝织物的弹性得到了很大的提高。真丝织物经二次拉伸后伸长率下降~30%;然而,在18次额外的拉伸-恢复试验后,伸长率几乎没有变化。它在其他纺织和非纺织应用中的使用。在之前的研究中,作为一项前期工作,用丝绸/聚氨酯包芯纱制成真丝纺织品,并用热水处理以提高其伸长率和弹性(Um, 2016)。然而,结果并不乐观,丝绸织物的弹性为96.7%,即使它只是延长了20%的伸长率。以真丝/聚氨酯包芯纱为原料,对真丝纺织品进行脱胶,以提高其弹性,考察脱胶对真丝纺织品结构和力学性能的影响。
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引用次数: 1
Effect of adult population density on egg production in the black soldier fly, Hermetia illucens (Diptera: Stratiomyidae) 成虫密度对黑兵蝇产卵量的影响(双翅目:层蛾科)
Pub Date : 2016-12-31 DOI: 10.7852/IJIE.2016.33.2.92
K. Park, W. Kim, Eun Sun Kim, Ji-Young Choi, Sung-hyun Kim
The black soldier fly is economically important because its prepupae are used as feed for many animals, including fish and swine. In Korea, black-soldier-fly farms have attempted to increase annual breeding and mass egg production for use in animal feed, as well as the decomposition of organic waste. Such efforts require an understanding of optimal mating and oviposition techniques. Specifically, adult densities and cage size may both improve the efficiency of mass egg production. Our study used four sizes of nylon cages (1.0 × 1.0 × 2.5 m, 1.5 × 1.5 × 2.5 m, 2.0 × 2.0 × 2.5 m, 2.5 × 2.5 × 2.5 m) and three density treatments (4 kg, 8 kg, and 10 kg of pupae) to investigate optimal habitat size and adult density. We found that cage size (independent of density) did not significantly influence female fecundity (number of egg clutches and egg weight), whereas higher densities increased egg number and weight regardless of cage size. Thus, we recommend manipulating adult density to enhance productivity in commercial black-soldier-fly farming. However, we also propose further detailed research to develop methods that account for seasonal changes and environmental conditions, as climatic variables (temperature, sunlight) likely influence female fecundity as well.
黑兵蝇在经济上很重要,因为它的预蛹可以作为许多动物的饲料,包括鱼和猪。在韩国,黑兵蝇农场试图增加每年的繁殖和用于动物饲料的大量鸡蛋产量,并分解有机废物。这种努力需要了解最佳交配和产卵技术。具体而言,成虫密度和笼型尺寸均可提高批量产蛋效率。本研究采用4种尺寸的尼龙笼(1.0 × 1.0 × 2.5 m、1.5 × 1.5 × 2.5 m、2.0 × 2.0 × 2.5 m、2.5 × 2.5 × 2.5 m)和3种密度处理(4 kg、8 kg和10 kg的蛹)来研究最佳生境大小和成虫密度。我们发现笼大小(与密度无关)对雌性繁殖力(卵窝数和卵重)没有显著影响,而无论笼大小如何,密度越高,卵数和卵重越高。因此,我们建议通过控制成虫密度来提高商业黑兵蝇养殖的生产力。然而,我们也建议进一步进行详细的研究,以制定考虑季节变化和环境条件的方法,因为气候变量(温度、阳光)也可能影响女性的繁殖力。
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引用次数: 9
Storage stability of silk solution for viscosity and electrospinnability 丝液粘度和电纺丝性能的贮存稳定性
Pub Date : 2016-12-31 DOI: 10.7852/IJIE.2016.33.2.138
S. Kim, I. Um
In the present study, silk fibroin (SF) was dissolved in CaCl 2 /H 2 O/EtOH solution at 85 o C. After the dissolution, the SF solution was cooled down and stored at 4 o C for 28 d. The stability of the solution’s viscosity and electrospinnability was observed to examine the stability of SF molecules during storage in CaCl 2 /H 2 O/EtOH solution. The viscosities of SF/CaCl 2 /H 2 O/ EtOH solution and SF formic acid solution did not change during 28 days’ storage of SF in CaCl 2 /H 2 O/EtOH solution. The electrospinnability of the SF solution, mean diameter of the electrospun SF fiber, and crystallinity index of electrospun SF web did not change, regardless of the length of the storage period. These results imply that SF molecules do not degrade during 28 days’ storage in CaCl 2 /H 2 O/EtOH solution.
本研究将丝素蛋白(SF)溶解在85℃的cacl2 / h2o /EtOH溶液中,溶解后冷却并在4℃下保存28 d,观察溶液粘度和电可纺性的稳定性,以考察丝素分子在cacl2 / h2o /EtOH溶液中储存的稳定性。SF/ cacl2 / h2o /EtOH溶液和SF甲酸溶液在cacl2 / h2o /EtOH溶液中储存28 d,粘度没有变化。无论贮存时间的长短,SF溶液的电可纺性、SF纤维的平均直径和SF网的结晶度指数都没有变化。这些结果表明,在cacl2 / h2o /EtOH溶液中,SF分子在28天内没有降解。
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引用次数: 1
Larval growth and the effect of temperature on head capsule size in Atrophaneura alcinous (Lepidoptera: Papilionidae) 黄萎蔫蝇幼虫生长及温度对其头囊大小的影响
Pub Date : 2016-12-31 DOI: 10.7852/IJIE.2016.33.2.50
Seonghyun Kim, Haechul Park, I. Park
The size of head capsule is one of the most important factors for identifying developmental stage. In order to understand the developmental characteristics of the butterfly Atrophaneura alcinous, we examined fluctuations in larval head capsule size under three different temperature conditions (20, 25, and 30°C) and 60% humidity. As a result, larvae developed to the fifth instar at all three temperatures. The head capsule size of larvae tended to be larger at the lowest temperature and smaller at the highest temperature. The development rate showed a regular change, consistent with Dyar’s rule regarding head capsule size development. Furthermore, the development of head capsule size was found to correspond to a second degree polynomial regression better than to a linear regression. On the basis of these results, it would appear to be possible to perform an accurate assessment of instar status during the development of A. alcinous.
头囊的大小是确定发育阶段的重要因素之一。为了了解萎蔫蝶(Atrophaneura alcinous)的发育特征,我们研究了三种不同温度条件(20、25和30℃)和60%湿度下幼虫头囊大小的变化。结果,在这三种温度下,幼虫都能发育到五龄。幼虫的头囊大小在最低温度下呈增大趋势,在最高温度下呈减小趋势。发育速率呈规律性变化,符合Dyar头囊大小发育规律。此外,发现头部蒴果大小的发展更符合二次多项式回归而不是线性回归。在这些结果的基础上,似乎有可能在A. alcinous的发育过程中进行准确的早期状态评估。
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引用次数: 0
Identification of the genes which related cold (low temperature) stress in Bombyx mori 家蚕冷(低温)胁迫相关基因的鉴定
Pub Date : 2016-12-31 DOI: 10.7852/IJIE.2016.33.2.102
M. Kang, Kwangho Choi, K. Park, S. Nho
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引用次数: 0
期刊
International journal of industrial entomology
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