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Influence of breed on motility and motion characteristics of fresh, chilled and frozen bull spermatozoa 品种对新鲜、冷藏和冷冻公牛精子运动特性的影响
Pub Date : 2021-03-31 DOI: 10.5897/IJLP2020.0700
Kefelegn Seyoum, Alemayehu Lemma, Asrat Tera
At present, frozen-thawed semen is used extensively for artificial insemination (AI) in Ethiopia. However, subjective semen quality assessment is still practiced by the semen processing organizations of the country. In this study, motility and motion characteristics of spermatozoa were assessed by using integrated semen analysis system (ISAS) to diagnose breed differences in quality of semen. Semen was collected from 14 breeding bulls (Boran = 4, Crosses of 75 % Holstein Frisian ˟ 25% Boran = 4 and Holstein Frisian = 6). After initial subjective assessment, the semen was evaluated at fresh, chilled and frozen stages for various sperm motion characteristics using integrated semen analysis system (ISAS® v1, Proiser, Spain). PH, volume and morphological defects were differed significantly (P < 0.05) among breeds. Significantly higher (P < 0.05) motility percentage (82.5%) was identified in Boran fresh semen. In contrast spermatozoa motion characteristics: Medium, medium progressive and slow types were significantly higher (P < 0.05) in crosses. Individual motility percentage and spermatozoa motion types (medium and slow) were significantly low (P < 0.05) in Boran chilled semen. In line with chilled semen significantly high motility percentage (42.9%) for frozen semen was observed in HF. The sperm kinematic parameters: Average path velocity (VAP, µm/s), straight line velocity (VSL, µm/s), curvilinear velocity (VCL, µm/s), amplitude of lateral head displacement (ALH, µm), beat cross frequency (BCF, Hz) and straightness (STR) percentage were differed significantly (P < 0.05) among the three breeds at all stages of semen production. The smallest kinematic values of all parameters except for LIN, STR and WOB of fresh semen were recorded in cross breed bulls at all stages of production. On the other hand significantly higher (P < 0.05) values for all the parameters (VCL, VAP, VSL, ALH, BCF, LIN, STR and WOB) of frozen semen were recorded in Boran breed. Thus it is possible to conclude that breed has influence on motility and motion characteristics of bull spermatozoa at different stages of semen production. Key words: Boran, Integrated Semen Analysis System (ISAS), kinematic parameters.
目前,冷冻解冻精液在埃塞俄比亚广泛用于人工授精(AI)。然而,我国精液加工机构仍然实行主观的精液质量评价。本研究采用精液综合分析系统(ISAS)对精子的活力和运动特性进行了评价,以诊断精液质量的品种差异。收集了14头种牛(博兰= 4,75%荷斯坦弗里斯兰杂交˟25%博兰= 4,荷斯坦弗里斯兰杂交= 6)的精液。在初步主观评估后,使用综合精液分析系统(ISAS®v1, Proiser,西班牙)在新鲜、冷藏和冷冻阶段评估精液的各种精子运动特征。不同品种间PH、体积和形态缺陷差异显著(P < 0.05)。柏然鲜精液的活动力率显著高于(P < 0.05) 82.5%。而精子运动特征:杂交中、中进行性、慢性显著增高(P < 0.05)。勃然冷藏精液的个体运动率和精子运动类型(中、慢)均显著低于对照组(P < 0.05)。与冷冻精液一致,在HF中观察到冷冻精液的高运动率(42.9%)。精子运动参数:平均路径速度(VAP,µm/s)、直线速度(VSL,µm/s)、曲线速度(VCL,µm/s)、侧头位移幅度(ALH,µm)、跳动交叉频率(BCF, Hz)和直线度(STR)百分比在3个品种的精液生产各阶段差异均显著(P < 0.05)。除新鲜精液的LIN、STR和WOB外,所有参数的运动学值在杂交公牛的各个生产阶段都是最小的。冻精VCL、VAP、VSL、ALH、BCF、LIN、STR和WOB均显著高于柏然品种(P < 0.05)。由此可以得出结论,品种对公牛精子在不同产精阶段的运动和运动特性有影响。关键词:Boran,精液综合分析系统(ISAS),运动学参数
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引用次数: 3
Effect of dietary hemp seed cake on the performance of commercial laying hens 饲粮中添加大麻籽饼对商品蛋鸡生产性能的影响
Pub Date : 2021-02-28 DOI: 10.5897/IJLP2020.0747
Rajasekhar Kasula, F. Solis, Byron Shaffer, Frank Connett, Chris Barrett, R. Cocker, E. Willinghan
The cultivation of hemp was prohibited due to its high content of the psychoactive substance Δ-9 tetrahydrocannabinol (THC). Regulatory changes from several countries allow the cultivation of hemp that permits plants and plant parts with less than 0.3% Δ-9 THC. The concern of the levels of THC still remains; therefore, testing hemp seed cake (HSC) contributes new information about the effect of this ingredient on livestock. The objective of this study was to evaluate the effect of HSC on feed intake, body weight (BW), egg production, eggs per hen housed (EHH), feed conversion ratio (FCR), and livability of laying hens. Eight hundred 30-week old laying hens were randomly distributed and fed four treatments for 19 weeks, no HSC (C0), 10% (H10), 20% (H20) and 30% (H30) of HSC replicated 8 times with 25 hens per replicate. Feed intake was not affected by the HSC supplementation. BWs were reduced across all treatments with significant lower impact on the HSC treatments, egg production, EHH and FCR were not consistently affected by HSC supplementation. The livability was within the normal range for the breed. The results of this study confirm that HSC supplementation in feed does not affect the performance of laying hens. Key words: Hemp, hemp seed cake (HSC), performance, tetrahydrocannabinol, tetrahydrocannabinol (THC), safety.
大麻因其精神活性物质Δ-9四氢大麻酚(THC)含量高而被禁止种植。几个国家的法规变化允许种植大麻,允许含有低于0.3% THC的植物和植物部分Δ-9。对四氢大麻酚含量的关注仍然存在;因此,测试大麻籽饼(HSC)为这种成分对牲畜的影响提供了新的信息。本试验旨在评价HSC对蛋鸡采食量、体重(BW)、产蛋量、每窝蛋数(EHH)、饲料系数(FCR)和宜居性的影响。选取30周龄蛋鸡800只,随机饲喂4个处理,分别饲喂不添加HSC (C0)、添加10% (H10)、20% (H20)和30% (H30)的HSC,重复8次,每个重复25只鸡。饲料采食量不受添加HSC的影响。所有处理均降低了体重,对HSC处理的影响显著降低,蛋产量、EHH和FCR不受补充HSC的影响。存活率在该品种的正常范围内。本研究结果证实,饲料中添加HSC对蛋鸡生产性能无影响。关键词:大麻,大麻籽饼,性能,四氢大麻酚,四氢大麻酚,安全性。
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引用次数: 4
Phenotypic characterization (qualitative traits) of various strains of indigenous Tswana chickens in Kweneng and Southern districts of Botswana 博茨瓦纳Kweneng和南部地区不同品种土着茨瓦纳鸡的表型特征(质量性状)
Pub Date : 2021-02-28 DOI: 10.5897/IJLP2020.0745
J. Machete, P. Kgwatalala, S. J. Nsoso, J. Morêki, Patrick G. Nthoiwa, A. O. Aganga
The aim of this study was to identify and describe qualitative traits of indigenous Tswana chicken populations in Kweneng and Southern districts of Botswana. The qualitative traits involved in the study included tail colour, breast colour, back colour, neck colour, comb type, shank colour, earlobe colour and head shape. Data were subjected to frequency and cross tabulation procedures of descriptive statistics in Statistical Package for Social Sciences (SPSS) to compute frequencies of occurrence of each qualitative trait. The five strains of indigenous Tswana chickens under scavenging management system showed distinct physical variations for most of the qualitative traits. Black was the most predominant tail colour across the strains (51.6%) followed by brown (27.9%). The frequency of brown breast colour and brown back colour were significantly higher in those respective regions. Brown and black were the predominant neck colours across the strains. The single comb type (81.7%), featherless shank (65.4%), red ear lobes (67.6%) and grey shank colour (32.9%) were the most predominant phenotypes across the strains. Plain and crested head shapes occurred at similar frequencies of 56.4 and 43.6%, respectively, in Tswana chickens in Southern part of Botswana. Key words: Botswana, morphological characterization, phenotypic variation, qualitative traits, Tswana chickens.
本研究的目的是鉴定和描述博茨瓦纳Kweneng和南部地区土着茨瓦纳鸡种群的质量特征。研究中涉及的定性特征包括尾巴颜色、胸部颜色、背部颜色、颈部颜色、梳子类型、小腿颜色、耳垂颜色和头部形状。数据采用社会科学统计软件包(SPSS)中描述性统计的频率和交叉表程序,以计算每个定性特征的发生频率。在清道夫管理制度下的5个土着茨瓦纳鸡品系在大多数质量性状上表现出明显的物理变异。尾色以黑色为主(51.6%),其次为棕色(27.9%)。棕胸色和棕背色的出现频率在这两个区域明显更高。棕色和黑色是所有品种的主要颈部颜色。单梳型(81.7%)、无羽柄型(65.4%)、红色耳垂型(67.6%)和灰色柄型(32.9%)是各菌株最主要的表型。在博茨瓦纳南部的茨瓦纳鸡中,平头型和冠头型出现的频率相似,分别为56.4和43.6%。关键词:博茨瓦纳,形态特征,表型变异,质量性状,茨瓦纳鸡
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引用次数: 2
Performance and welfare of dairy cattle in an alternative compost bedded pack housing in a pasture-based system 牧场系统中替代堆肥床状包装房舍中奶牛的生产性能和福利
Pub Date : 2021-01-31 DOI: 10.5897/IJLP2020.0749
S. K. Bettie
The objective of this study was to assess the performance and welfare (lameness, hock lesions, mastitis) of loose-housed dairy cattle managed under grazing or semi-grazing system. Data of thirty-two cows before and after changing to semi-grazing system during the rainy season (week 1 to 4; grazing, week 6 to 9; semi-grazing) and before and after changing to semi-grazing (week 11 to 16; grazing, week 18 to 23, semi-grazing) during the dry season were examined. Cows were evaluated weekly for 24 weeks for somatic cell count (SCC), lameness, hock lesions, and hygiene. Milk yield was collected daily. Bacteria cultures were prepared to identify pathogens. Isolates were subjected to sensitivity tests. No difference was observed between grazing and semi-grazing, regarding milk yield, lameness, hock lesions, SCC, and sub-clinical mastitis prevalence. Mean SCC under grazing was >600,000 cells/ml, indicating infection with major pathogens (Streptococcus agalatiae, Streptococcus dysgalatiae). Klebsiella species increased with season and were less sensitive to common antibiotics. Contagious pathogens in milk were lower (14%) compared with environmental pathogens (82%). Because Klebsiella species were less sensitive to many antibiotics, keeping cows healthy is critical. Focus on reducing somatic cell count will be important in preventing mastitis infection in dairy cows. Key words: Grazing, semi-grazing system, performance, cattle welfare.
本研究的目的是评估放牧或半放牧制度下散养奶牛的生产性能和福利(跛行、飞节病变、乳腺炎)。雨季改半放牧前后32头奶牛数据(第1 ~ 4周;放牧,第6 - 9周;半放牧)和半放牧前后(第11 ~ 16周;旱季放牧(第18 ~ 23周,半放牧)。连续24周,每周对奶牛进行体细胞计数(SCC)、跛行、飞节病变和卫生状况评估。每天收集产奶量。准备细菌培养物以鉴定病原体。分离物进行敏感性试验。放牧和半放牧在产奶量、跛行、跗关节病变、SCC和亚临床乳腺炎患病率方面没有观察到差异。放牧条件下平均SCC >600,000细胞/ml,提示感染了主要病原菌(无乳链球菌、失乳链球菌)。克雷伯菌种类随季节增加,对常用抗生素不敏感。牛奶中的传染性病原体(14%)低于环境病原体(82%)。由于克雷伯氏菌对许多抗生素不太敏感,因此保持奶牛的健康至关重要。减少奶牛的体细胞计数是预防奶牛乳腺炎感染的重要方法。关键词:放牧;半放牧制度;生产性能;
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引用次数: 0
Comparative study on efficiency of sexed semen and conventional semen on in vivo produced bovine embryo quality and quantity of Boran and Holstein -Boran cross bred in Bishoftu, Ethiopia 埃塞俄比亚比绍夫图Boran和荷尔斯泰因-Boran杂交牛体内产胚质量和数量的两性精液和常规精液效率比较研究
Pub Date : 2021-01-31 DOI: 10.5897/IJLP2020.0751
Mosisa Dire Babura, T. Degefa, Fekadu Reggasa
The study was conducted from December 2019 to May 2020 to evaluate the efficiency of local convectional semen and imported sexed semen on in vivo produced bovine embryo quality in Boran and HB cross breed donor cows in Bishoftu, Ethiopia. In this study, it was hypothesized that the breed of donor cows and types of semen can influence the quality of in vivo produced embryo as well as the number of transferable embryos. Randomized experimental design was employed, and a total of 20 donor cows (10 Boran and 10H*B cross) were superovulated and flushed excluding non-responsive donor cows. Donor cows were randomly assigned to two groups: Group-1- five donors inseminated with sexed semen and group-2- five other cows inseminated with convectional semen from each breed. The results of study showed that the embryo recovery rate was 68% in H*B crossbreds and 53% in Boran donor cows. A total of 121 embryos were produced of which, 60 were transferable embryos (36 from H*B crossbreds and 24 from Boran), 39 UFOs (31 from H*B crossbreds and 8 from Boran) and 22 degenerated embryos (12 from HB cross and 10 from Boran). The results of this study showed that the number of transferable embryos produced from donors inseminated with sexed semen and convectional semen was nearly similar. However, the number of defective embryos tended to be higher with sexed semen and the number of unfertilized oocytes is significantly (p<0.05) higher in crossbred cows. This finding suggests that farmer can use sexed semen to get sex specific dairy calves in both cattle genotypes. Key words: Breed, donor cows, superovulation.
该研究于2019年12月至2020年5月在埃塞俄比亚比绍夫图进行,旨在评估当地常规精液和进口性精液对Boran和HB杂交供体牛体内生产的牛胚胎质量的影响。在本研究中,假设供体奶牛的品种和精液的类型会影响体内产生的胚胎的质量以及可移植胚胎的数量。采用随机试验设计,选取20头供体奶牛(10头博然与10头h *B杂交)进行超排卵和冲洗试验,排除无反应供体奶牛。将供体奶牛随机分为两组:组1- 5只供体奶牛用性授精,组2- 5只供体奶牛用每个品种的常规精液授精。研究结果表明,H*B杂交奶牛的胚胎恢复率为68%,柏然供牛的胚胎恢复率为53%。共获得121个胚胎,其中可移植胚胎60个(H*B杂交36个,博然24个),UFOs胚胎39个(H*B杂交31个,博然8个),退化胚胎22个(HB杂交12个,博然10个)。本研究结果表明,用有性精子和常规精子受精的供体产生的可移植胚胎数量几乎相同。杂交奶牛的缺陷胚胎数量倾向于用有性精子增加,未受精卵细胞数量显著(p<0.05)增加。这一发现表明,在两种牛的基因型中,农民都可以使用性别化的精液来获得性别特异性的奶牛犊牛。关键词:品种,供牛,超排卵。
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引用次数: 0
Effect of garlic (Allium sativum) and onion (Allium cepa L.) extract chitosan nanoparticles on antioxidant enzymes activities and relative weight of visceral organs of rainbow rooster chicken 大蒜(Allium sativum)和洋葱(Allium cepa L.)提取物壳聚糖纳米粒对彩虹公鸡鸡内脏器官抗氧化酶活性和相对重量的影响
Pub Date : 2020-12-31 DOI: 10.5897/IJLP2020.0728
Viviana Imbua Levi Enoka, G. Kikuvi, P. Ndung'u
Poultry meat is susceptible to oxidation but increased antioxidant enzyme increases its availability in muscle during processing and storage. In livestock, synthetic antioxidant has some side effects; plant polyphenols can enhance the level of antioxidant enzymes but they are in-active in the gut of chicken and therefore, nanotechnology can be of importance in augmenting the stability of polyphenols. In the study, seventy two rainbow rooster chickens were treated with nanoparticles- prepared from Chitosan with Aqueous Garlic and Onion (CHIAGO), chitosan with total phenol and ajoene rich extract (CHITPA), and chitosan solution (CHISOLN) of 5 to 10%, with 0.5 g and 1 g Fosbac (antibiotic) administered orally twice a week for a period of 8 weeks. One chicken from each of the group and a control group were sacrificed on weekly basis with the muscles and visceral organs removed for analysis. The weight of visceral organs, catalase (CAT) enzyme activities of thigh and breast were analyzed from 1st to 8th weeks and 2, 2-Diphenyl-1-picryl hydroxyl (DPPH) inhibition of thigh and breast muscles for 1st, 4th and 8th weeks. Relative weight of the heart, liver and spleen did not change when compared with the control (p>0.05) but it increased in the gizzard (p 0.05) but without increase in the erythrocytes and liver (p>0.05). DPPH inhibition increased with CHIAGO 10%, CHITPA 10% in week 4 and week 8 in the breast and thigh muscle. Garlic and onion extract chitosan nanoparticles can act as natural antioxidant compound. Key words: Chitosan, garlic and onion extract, visceral organ, meat, antioxidant enzyme activities.
禽肉易受氧化,但在加工和储存过程中,增加抗氧化酶可提高其在肌肉中的利用率。在家畜中,合成抗氧化剂有一定的副作用;植物多酚可以提高抗氧化酶的水平,但它们在鸡的肠道中是不活跃的,因此,纳米技术在增强多酚的稳定性方面可能是重要的。本研究以72只彩虹鸡为实验对象,分别用大蒜和洋葱水壳聚糖(CHIAGO)、富含总酚和ajoene提取物的壳聚糖(CHITPA)和壳聚糖溶液(CHISOLN)(5% ~ 10%)制备纳米颗粒,每周口服两次Fosbac(抗生素)0.5 g和1g,持续8周。每组1只,对照组1只,每周处死1只,取肌肉和内脏器官进行分析。在试验第1 ~ 8周,分别测定各组大鼠脏器重量、大腿和乳房过氧化氢酶(CAT)活性和第1、4、8周大腿和乳房肌肉2,2 -二苯基-1-苦硝基羟基(DPPH)抑制情况。与对照组相比,心脏、肝脏和脾脏的相对重量没有变化(p>0.05),砂囊的相对重量增加(p 0.05),红细胞和肝脏的相对重量没有增加(p>0.05)。在第4周和第8周,胸部和大腿肌肉DPPH的抑制作用随着CHIAGO 10%、CHITPA 10%的增加而增加。大蒜和洋葱提取的壳聚糖纳米颗粒可作为天然抗氧化化合物。关键词:壳聚糖,大蒜洋葱提取物,内脏器官,肉类,抗氧化酶活性
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引用次数: 2
Native chicken farming: A tool for wealth creation and food security in Benin 本地养鸡:贝宁创造财富和粮食安全的工具
Pub Date : 2020-11-30 DOI: 10.5897/ijlp2020.0716
A. Camus, F. Arthur, Osei-Amponsah Richard, D. Nourou, Gbedevi Robert, Fonton Marie-Christelle, J. Guilherme, S. Farougou
Poultry is the second largest livestock in Benin (contributing to 22% of the total meat produced), and chickens are the most reared poultry in the country. The objective of this study was to assess the current trends of local chicken breeding and factors that influence their production performance, and thereby to deliver information for production improvement and sustainable use of indigenous chicken breeds. An electronic tool was used to collect information from 269 respondents selected through a chain referral sampling method. The results showed that there were three production systems; however, the most common production system used was free-range extensive system (70%). Regarding flock ownership, men owned the birds in the majority of the cases (63%) and had overall higher population of chickens than women. The most predominant constraints for chicken production, in descending order of importance, included theft, access to bank loan, lack of training, limited investment, disease spread and mortality. From our results, gender, financial resources, main activity of the flock owner, weaning practice, and the production area had a significant effect on flock size. To enhance the indigenous chicken production, changes in traditional management practices combined with breeding program focusing on within breed selection (closed nuclear system) is a better approach.  Key words: Biodiversity, cluster analysis, food security, farmer livelihood, local chickens.
家禽是贝宁第二大家畜(占肉类总产量的22%),而鸡是该国饲养最多的家禽。本研究的目的是评估地方鸡养殖的当前趋势和影响其生产性能的因素,从而为改进生产和可持续利用地方鸡品种提供信息。通过连锁推荐抽样方法,使用电子工具从269名受访者中收集信息。结果表明:该区存在三种生产体系;然而,最常见的生产系统是散养放养系统(70%)。在鸡群所有权方面,男性在大多数情况下拥有鸡(63%),并且鸡的总体数量高于女性。鸡肉生产最主要的制约因素(按重要性由高到低)包括盗窃、无法获得银行贷款、缺乏培训、投资有限、疾病传播和死亡率。从我们的研究结果来看,性别、经济资源、畜群主的主要活动、断奶方式和生产区域对畜群规模有显著影响。为了提高地方鸡的产量,改变传统的管理做法,结合以品种内选择(封闭核系统)为重点的育种计划是更好的方法。关键词:生物多样性,聚类分析,粮食安全,农民生计,地方鸡
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引用次数: 3
Laboratory evaluation, purpose of production and utilization of cattle manure in enset (Ensete ventricosum) based mixed production systems of Gurage Zone, Southern Ethiopia 埃塞俄比亚南部古拉格地区以牛粪为基础的混合生产系统的实验室评价、生产目的和牛粪利用
Pub Date : 2020-11-30 DOI: 10.5897/ijlp2019.0609
Dirsha Demam Wonchesa
The study was conducted in four districts of Gurage zone in Sothern Ethiopia to determine the N, P, K, organic carbon and nitrogen of cattle manure. Seventy two households, 36 from highland and 36 from midaltitude were randomly selected from a total of 360 sample households selected for the study. Around 88.05% of farmers in the study areas were keeping cattle for high demand of manure to fertilize enset fields and for milk production. There were no practices of using cattle dung as fuel or dung cake for sale. The sampled manure had nitrogen content of 2.68% and C/N ratio of 11:1 in highland and 2.24% with C/N ratio of 12:1 in mid-altitude. The organic matter obtained from manure in highland and midaltitude, respectively, were 51.89 and 44.82%. The gram of N, P, K, kg-1 DM of manure, respectively, were 26.8, 16.5 and 1.6 for highland and 22.4, 12.6 and1.2 for midaltitude. To realize production sustainability of enset system, cattle manure was found to be of paramount importance. Therefore, appropriate interventions in cattle production and forage development are the prime necessity to realize sustainability in enset production and households’ food security. Key words: Cattle manure, Gurage Zone NPK, organic carbon, organic matter.
本研究在埃塞俄比亚南部古拉格地区的4个地区进行,测定牛粪中的N、P、K、有机碳和氮。从360个样本家庭中随机抽取72户,其中高原36户,中等海拔36户。在研究地区,约88.05%的农民养牛是为了给农田施肥和生产牛奶需要大量的肥料。没有将牛粪用作燃料或牛粪饼出售的做法。高原地区土壤氮素含量为2.68%,碳氮比为11:1;中等海拔地区土壤氮素含量为2.24%,碳氮比为12:1。高原和中高原有机肥有机质含量分别为51.89%和44.82%。高原地区N、P、K、kg-1 DM分别为26.8、16.5和1.6克,中等海拔地区为22.4、12.6和1.2克。为了实现养殖系统的可持续生产,牛粪是至关重要的。因此,对牛生产和饲料发展进行适当干预是实现畜群生产可持续性和家庭粮食安全的首要必要条件。关键词:牛粪;牧区氮磷钾;有机碳;
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引用次数: 1
Metagenomic assessment of the rumen resistome, mobilome and stress response genes in smallholder dairy cattle in Kenya 肯尼亚小农奶牛瘤胃抵抗组、移动组和应激反应基因的宏基因组评估
Pub Date : 2020-11-30 DOI: 10.5897/ijlp2019.0654
Kibegwa Felix, Bett C. Rawlynce, G. Charles, Machuka Eunice, Mujibi D. Fidalis
Smallholder dairy cattle rumen microbiotas are subjected to a wide range of antimicrobials as well as sudden fluctuations in diets. As such, they develop an enormous reservoir of resistant genes, mobilome and stress response genes. However, information on metagenomic reactions to such dietary variations, especially for cattle reared in the tropics, remains largely unexplored. This meta-analysis was conducted to assess if antibiotic and toxic compound resistance genes (ARGs), stress response genes and bacterial phages, prophages and transposable element genes were present, and to what extent, in three dairy cattle genotypes (Friesian, FriesianXJersey crossbreed, Jersey) reared in a farm that practiced judicious use of antimicrobials. Potential bacterial hosts to these genes were also explored. The rumen metagenomes generated from Next Generation Sequencing (NGS) technology were analyzed using MG-RAST. According to the results stress reaction, resistance and mobilome genes were present in similar amounts in all the three genotypes. Cobalt-zinc-cadmium resistance, fluoroquinolone resistance, methicillin resistance in Staphylococci and multidrug resistance efflux pumps were the most abundant resistant genes and were spread across 20, 24, 16 and 21 bacterial classes, respectively. Bacteria in charge of phage integration and excision, phages replication and phage packaging were mostly allocated to the phyla Firmicutes, Bacteroides and Proteobacteria. Within the stress response genes, metagenomic assembly-based host-tracking analysis identified the extended heat shock dnaK gene cluster as the most abundant genes, while Bacteroides and Clostridium were the principal bacterial hosts. The results show that even with proper use of antimicrobials, the cattle rumen contained an immense distribution of responses to stress, ARGs and mobilome genes distributed in a vast assemblage of hosts. There is also a high correlation between these three functional groups. Key words: Resistome, metagenome, MG-RAST, Stress genes, mobilome
小农奶牛瘤胃微生物群受到各种抗菌剂和饲料突然波动的影响。因此,它们形成了一个巨大的抗性基因、移动组和应激反应基因库。然而,关于这种饮食变化的宏基因组反应的信息,特别是对于在热带地区饲养的牛,在很大程度上仍未被探索。本荟萃分析旨在评估在合理使用抗菌剂的养殖场饲养的三种奶牛基因型(弗里西亚、弗里西亚与泽西杂交、泽西)中是否存在抗生素和有毒化合物耐药基因(ARGs)、应激反应基因以及细菌噬菌体、噬菌体和转座因子基因,以及在多大程度上存在。对这些基因的潜在细菌宿主也进行了探索。利用MG-RAST对下一代测序(NGS)技术生成的瘤胃宏基因组进行分析。结果表明,应激反应基因、抗性基因和运动组基因在三种基因型中含量相近。耐钴锌镉、氟喹诺酮类、葡萄球菌耐甲氧西林和耐多药外排泵是最丰富的耐药基因,分别分布在20、24、16和21个细菌类别中。负责噬菌体整合和切除、噬菌体复制和噬菌体包装的细菌主要分布在厚壁菌门、拟杆菌门和变形菌门。在胁迫响应基因中,基于宏基因组组装的宿主跟踪分析发现,扩展热休克dnaK基因簇是最丰富的基因,而拟杆菌和梭状芽胞杆菌是主要的细菌宿主。结果表明,即使在适当使用抗菌剂的情况下,牛瘤胃中也含有大量的应激反应基因,ARGs和移动组基因分布在大量宿主中。这三个官能团之间也有很高的相关性。关键词:抗性组,宏基因组,MG-RAST,应激基因,移动组
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引用次数: 0
Urban dairy production and waste management in Oromia special zone around Finfine, Ethiopia 埃塞俄比亚芬芬附近奥罗米亚特区的城市乳制品生产和废物管理
Pub Date : 2020-10-31 DOI: 10.5897/ijlp2020.0718
Jalel Fikadu, B. Tamir, U. Galmessa, K. Effa
The survey was conducted to assess urban dairy production and waste management system in Oromia Special Zone around Finfinnee, Ethiopia. The three study towns (Burayu, Sululta and Sebeta) were purposively selected due to the high potential for commercial dairy production. A total of 90 commercial dairy producers 30 from each town who at least own 10 dairy cows were randomly selected. The farmers interviewed individually using the survey questionnaire. The collected data were analyzed and the study revealed that 47.8 and 52.2% of the interviewed were female and male respectively. Next to daily laborers, household wives shared larger responsibility for feeding (21.1%), milking (28.9%) and cleaning (13.3%). The genetic composition of dairy cows in the study areas ranges from 50% exotic gene inheritances to pure (100%) exotic Holstein Friesian. Accordingly, 50, 62.5, ≥75% and pure Holstein Friesian cows account for about 24.4, 38.9, 24.4 and 11% of the herd, respectively. The major sources of feed were both formulated feed and feed that mixed at home (55.6%) and tap water (74. 4%). The average age at first calving, calving interval and days open was 2.26±.05years 20.8 ± 0.05 months and 161.76±34.80 days respectively. The major waste in the farm is manure (73.3%) and followed by feed left over (14.45%) and dust (12. 25%). High price feed, shortage of land, unavailability of dairy cow/heifer in time, feed quality, unavailability of feed in nearby area, diseases and lack of access to credit, shortage of water and inadequate training were among the major constraint of dairy production that need urgent intervention to utilize the untapped resources in the area. Key words: Dairy cattle, urban, production, milk, waste.
该调查旨在评估埃塞俄比亚Finfinnee周围奥罗米亚特区的城市乳制品生产和废物管理系统。这三个研究城镇(Burayu, Sululta和Sebeta)是有目的地选择的,因为它们具有商业乳制品生产的巨大潜力。随机抽取90名商业奶农,每个镇30名,至少拥有10头奶牛。使用调查问卷对农民进行了个别访谈。对收集到的数据进行分析,研究发现受访者中女性和男性分别占47.8%和52.2%。除了日工之外,家庭主妇承担的喂养(21.1%)、挤奶(28.9%)和清洁(13.3%)的责任更大。研究地区奶牛的基因组成从50%的外来基因遗传到纯(100%)外来荷斯坦弗里西亚奶牛。据此,50头、62.5头、≥75%头和纯种荷斯坦弗里西亚奶牛分别约占畜群的24.4%、38.9%、24.4%和11%。饲料的主要来源是配方饲料和家中混合饲料(55.6%)和自来水(74%)。4%)。平均初产犊龄、产犊间隔和开胎天数为2.26±。05年,20.8±0.05个月,161.76±34.80天。猪场的主要废物是粪肥(73.3%),其次是饲料残渣(14.45%)和粉尘(12.3%)。25%)。饲料价格高、土地短缺、奶牛/小母牛无法及时获得、饲料质量、附近地区无法获得饲料、疾病和无法获得信贷、缺水和培训不足是乳制品生产的主要制约因素,需要紧急干预,以利用该地区未开发的资源。关键词:奶牛;城市;生产;
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引用次数: 1
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International Journal of Livestock Production
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