Pub Date : 2024-03-01DOI: 10.22159/ijpps.2024v16i3.48975
Mitali Dua, Arvind Narwat, Abhinav Goyal
Objective: Drug-induced cutaneous reactions are common problem in our country and can range from simple rash to severe reactions. Early recognition of these reactions enables early identification and withdrawal of offending drugs, thereby reducing morbidity and mortality. So present study aimed to assess clinical pattern of drug-induced cutaneous reactions in Dermatology OPD. Methods: This study was an open, non-comparative, non-interventional, observational study conducted on patients visiting dermatology department to see the clinical pattern of drug-induced cutaneous reactions. A total of 60 patients with suspected cutaneous adverse drug reactions were recruited. A detailed physical examination was done by a physician, including drug intake during 3 w preceding reactions and type of drug reactions. Results: Most frequently reported cutaneous drug reactions were Stevens-Johnson Syndrome (23%), Maculopapular rash (18%) Toxic Epidermal Necrolysis (15%) and were caused by antiepileptic drugs in 21(35%) patients, followed by antibiotics in 17(28.33%) cases, NSAID’s in 7(11.6%) cases, antitubercular drugs in 3(5%) and antiretroviral drugs in 3(5%) cases. A high proportioned of these reactions (50%) were moderate (31%) of these were severe because they require hospitalisation or increased the duration of stay in hospital or were life-threatening in (1%). Principal offending drug was phenytoin. Conclusion: A good knowledge of ADRs, a careful history taking and watchful approach while prescribing of drugs can prevent many of adverse drug reactions. These facts justify the development of an intensive programme of pharmacovigilance.
{"title":"DRUG-INDUCED CUTANEOUS REACTIONS: A PHARMACOVIGILANCE STUDY","authors":"Mitali Dua, Arvind Narwat, Abhinav Goyal","doi":"10.22159/ijpps.2024v16i3.48975","DOIUrl":"https://doi.org/10.22159/ijpps.2024v16i3.48975","url":null,"abstract":"Objective: Drug-induced cutaneous reactions are common problem in our country and can range from simple rash to severe reactions. Early recognition of these reactions enables early identification and withdrawal of offending drugs, thereby reducing morbidity and mortality. So present study aimed to assess clinical pattern of drug-induced cutaneous reactions in Dermatology OPD.\u0000Methods: This study was an open, non-comparative, non-interventional, observational study conducted on patients visiting dermatology department to see the clinical pattern of drug-induced cutaneous reactions. A total of 60 patients with suspected cutaneous adverse drug reactions were recruited. A detailed physical examination was done by a physician, including drug intake during 3 w preceding reactions and type of drug reactions.\u0000Results: Most frequently reported cutaneous drug reactions were Stevens-Johnson Syndrome (23%), Maculopapular rash (18%) Toxic Epidermal Necrolysis (15%) and were caused by antiepileptic drugs in 21(35%) patients, followed by antibiotics in 17(28.33%) cases, NSAID’s in 7(11.6%) cases, antitubercular drugs in 3(5%) and antiretroviral drugs in 3(5%) cases. A high proportioned of these reactions (50%) were moderate (31%) of these were severe because they require hospitalisation or increased the duration of stay in hospital or were life-threatening in (1%). Principal offending drug was phenytoin.\u0000Conclusion: A good knowledge of ADRs, a careful history taking and watchful approach while prescribing of drugs can prevent many of adverse drug reactions. These facts justify the development of an intensive programme of pharmacovigilance.","PeriodicalId":14188,"journal":{"name":"International Journal of Pharmacy and Pharmaceutical Sciences","volume":"101 19","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140088891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-01DOI: 10.22159/ijpps.2024v16i3.50326
Barnali Mandal
Chemical preservatives, usually used during the long period, to protect the food materials by controlling undesirable bacteria and harmful spoiler, have been proved as toxic to human health. Conscious consumers have serious awareness to purchase safe foods without chemical additives. In the recent years, bio-preservation is gained increasing attention to harmonize consumer demands along with standard food quality. Various attempts are growing on the use of micro-organisms or their antimicrobial metabolites for the protection of food products. The bacteriocins produced by lactic acid bacteria (LAB) have a relatively broad antimicrobial spectrum against variety of food-borne pathogenic and spoilage bacteria. Bacteriocin-producing lactic acid bacteria or bacteriocins can be used in foods as bio-preservatives. The review is focused on bacteriocin produced by lactic acid bacteria.
{"title":"BACTERIOCIN PRODUCED BY LACTIC ACID BACTERIA: A PROBIOTIC","authors":"Barnali Mandal","doi":"10.22159/ijpps.2024v16i3.50326","DOIUrl":"https://doi.org/10.22159/ijpps.2024v16i3.50326","url":null,"abstract":"Chemical preservatives, usually used during the long period, to protect the food materials by controlling undesirable bacteria and harmful spoiler, have been proved as toxic to human health. Conscious consumers have serious awareness to purchase safe foods without chemical additives. In the recent years, bio-preservation is gained increasing attention to harmonize consumer demands along with standard food quality. Various attempts are growing on the use of micro-organisms or their antimicrobial metabolites for the protection of food products. The bacteriocins produced by lactic acid bacteria (LAB) have a relatively broad antimicrobial spectrum against variety of food-borne pathogenic and spoilage bacteria. Bacteriocin-producing lactic acid bacteria or bacteriocins can be used in foods as bio-preservatives. The review is focused on bacteriocin produced by lactic acid bacteria.","PeriodicalId":14188,"journal":{"name":"International Journal of Pharmacy and Pharmaceutical Sciences","volume":"27 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140277675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-01DOI: 10.22159/ijpps.2024v16i3.50234
Ridhdhi K. Hirapara, Anil Singh, B. Karelia, Anita Sinha
Objective: Pemphigus Vulgaris [PV] is a chronic autoimmune disease. Corticosteroids and Immunosuppressants are the main line of treatment. The aim of this study was to evaluate the prescription pattern and patient adherence behavior with therapy. We determined the association between patient adherence and quality of life in patients with pemphigus vulgaris. Methods: A prospective observational study was conducted from February 2020 to May 2021. The age and sex of patients who were diagnosed with PV within 1 mo period and confirmative histopathological findings in Biopsy were included in the study. A total of 35 patients were analyzed and patients were reviewed for medication adherence and quality of life by using MMAS-8 and WHOQOL respectively, monthly till 3 mo after initiation of therapy. Statistical analysis was done by using Epi Info version 7.1.5. Results: Out of 140 prescriptions, vitamin C and zinc were the most common drug prescribed in 129 prescriptions followed by prednisolone prescribed in 128 prescriptions. The average number of drugs per encounter was 8.67. 97.56 % of drugs were prescribed by generic names. No significant improvement was seen in the adherence behavior of patients from 1st follow-up to 3rd follow-up. It was observed that physical, psychological, and environmental domains have significant associations with medication adherence in all 3 follow-up visits. Conclusion: It was concluded that Polypharmacy and inappropriate use of medicines may decrease the adherence behavior of patients to the therapy. Low adherence to pharmacotherapy affects the quality of life in PV patients. This study is beneficial for raising awareness about treatment adherence and also encouraging the development of appropriate interventions.
{"title":"AN ANALYSIS OF PRESCRIPTION PATTERN, ADHERENCE TO PHARMACOTHERAPY AND QUALITY OF LIFE IN PEMPHIGUS VULGARIS PATIENTS","authors":"Ridhdhi K. Hirapara, Anil Singh, B. Karelia, Anita Sinha","doi":"10.22159/ijpps.2024v16i3.50234","DOIUrl":"https://doi.org/10.22159/ijpps.2024v16i3.50234","url":null,"abstract":"Objective: Pemphigus Vulgaris [PV] is a chronic autoimmune disease. Corticosteroids and Immunosuppressants are the main line of treatment. The aim of this study was to evaluate the prescription pattern and patient adherence behavior with therapy. We determined the association between patient adherence and quality of life in patients with pemphigus vulgaris.\u0000Methods: A prospective observational study was conducted from February 2020 to May 2021. The age and sex of patients who were diagnosed with PV within 1 mo period and confirmative histopathological findings in Biopsy were included in the study. A total of 35 patients were analyzed and patients were reviewed for medication adherence and quality of life by using MMAS-8 and WHOQOL respectively, monthly till 3 mo after initiation of therapy. Statistical analysis was done by using Epi Info version 7.1.5.\u0000Results: Out of 140 prescriptions, vitamin C and zinc were the most common drug prescribed in 129 prescriptions followed by prednisolone prescribed in 128 prescriptions. The average number of drugs per encounter was 8.67. 97.56 % of drugs were prescribed by generic names. No significant improvement was seen in the adherence behavior of patients from 1st follow-up to 3rd follow-up. It was observed that physical, psychological, and environmental domains have significant associations with medication adherence in all 3 follow-up visits.\u0000Conclusion: It was concluded that Polypharmacy and inappropriate use of medicines may decrease the adherence behavior of patients to the therapy. Low adherence to pharmacotherapy affects the quality of life in PV patients. This study is beneficial for raising awareness about treatment adherence and also encouraging the development of appropriate interventions.","PeriodicalId":14188,"journal":{"name":"International Journal of Pharmacy and Pharmaceutical Sciences","volume":"2 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140270449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-01DOI: 10.22159/ijpps.2024v16i3.49807
N. Karunasree, Faheem Begum, Mohammed OBAIDULLAH KHAN, Khatijatul Kubra Nameera
Objective: To compare the efficacy and safety of topical ciprofloxacin v/s fortified gentamicin-ceftazidime in bacterial keratitis. Methods: This was a randomized control trial done on 60 subjects with 30 subjects in each group. The clinical signs and symptoms are recorded in two groups of bacterial keratitis patients at baseline and after 2 w of treatment using ciprofloxacin ophthalmic solution and standard therapy regimen of fortified gentamicin-ceftazidime using scoring of ocular signs and symptoms (1=minimum, not present), (5=maximum, severe) with a study period of 3 mo Results: The group administered with fortified ceftazidime+gentamicin demonstrated superior clinical and statistical efficacy compared to ciprofloxacin in treating bacterial keratitis. This regimen led to a substantial alleviation of symptoms and minimized ocular discomfort to a greater extent. Notably, the calculated p-value for the day 14 score, standing at 0.02 (below the 0.05 threshold), underscores the significant superiority of fortified ceftazidime+gentamicin in symptom reduction. Conclusion: We conclude that fortified ceftazidime+gentamicin is better than ciprofloxacin for the treatment of bacterial keratitis.
{"title":"A COMPARATIVE STUDY TO EVALUATE EFFICACY AND SAFETY OF TOPICAL CIPROFLOXACIN V/S FORTIFIED GENTAMICIN-CEFTAZIDIME IN BACTERIAL KERATITIS” AT A TERTIARY CARE CENTRE IN TELANGANA","authors":"N. Karunasree, Faheem Begum, Mohammed OBAIDULLAH KHAN, Khatijatul Kubra Nameera","doi":"10.22159/ijpps.2024v16i3.49807","DOIUrl":"https://doi.org/10.22159/ijpps.2024v16i3.49807","url":null,"abstract":"Objective: To compare the efficacy and safety of topical ciprofloxacin v/s fortified gentamicin-ceftazidime in bacterial keratitis.\u0000Methods: This was a randomized control trial done on 60 subjects with 30 subjects in each group. The clinical signs and symptoms are recorded in two groups of bacterial keratitis patients at baseline and after 2 w of treatment using ciprofloxacin ophthalmic solution and standard therapy regimen of fortified gentamicin-ceftazidime using scoring of ocular signs and symptoms (1=minimum, not present), (5=maximum, severe) with a study period of 3 mo\u0000Results: The group administered with fortified ceftazidime+gentamicin demonstrated superior clinical and statistical efficacy compared to ciprofloxacin in treating bacterial keratitis. This regimen led to a substantial alleviation of symptoms and minimized ocular discomfort to a greater extent. Notably, the calculated p-value for the day 14 score, standing at 0.02 (below the 0.05 threshold), underscores the significant superiority of fortified ceftazidime+gentamicin in symptom reduction.\u0000Conclusion: We conclude that fortified ceftazidime+gentamicin is better than ciprofloxacin for the treatment of bacterial keratitis.","PeriodicalId":14188,"journal":{"name":"International Journal of Pharmacy and Pharmaceutical Sciences","volume":"248 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140275600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-01DOI: 10.22159/ijpps.2024v16i3.50391
Chitra Selvarajan, Nalini Ganesan
Objective: Chalcones and their derivatives display a wide range of pharmacological activities. This study examined the effects of AM114, a boronic-chalcone derivative, on human THP-1-derived macrophages with and without interleukin-1β (IL-1β) stimulation. Methods: AM114 and Aspirin-treated THP-1-derived macrophages underwent activation with or without interleukin-1β. The IC50 concentrations of AM114 and Aspirin were determined through an MTT test. Apoptosis was measured using various techniques, including staining with acridine orange/Ethidium bromide, Hoechst 33342, and rhodamine 123 assays. Caspase-3 activity was measured using the spectrofluorimetric technique, while DNA fragmentation was assessed via agarose gel electrophoresis. Pro-inflammatory cytokines such as interleukin-6 (IL-6) and chemokines like interleukin-8 (IL-8) were measured using enzyme-linked immunosorbent assays. Results: AM114 and Aspirin showed dose-dependent cytotoxic effects on THP-1 macrophages. Induction of apoptosis was detected in AM114-treated THP-1 macrophages activated with IL-1β compared to macrophages without IL-1β. The gradation of dye uptake, membrane blebbing, increased caspase-3 activity, and DNA fragmentation ensures the induction of apoptosis, which indicates the cell's morphological changes, biochemical processes, and mitochondrial activity. Treating AM114 in IL-1β-activated THP-1 macrophages significantly reduced pro-inflammatory cytokines (IL-6) and chemokines (IL-8), suggesting its anti-cytokine potential in inflammatory diseases. Conclusion: The study results emphasize that AM114 could act as an anti-inflammatory agent by triggering apoptosis and reducing the release of cytokines and chemokines in inflammatory conditions. As a result, it may be used as a therapeutic option for inflammatory diseases.
{"title":"EXPLORING THE THERAPEUTIC POTENTIAL OF AM114: A BORONIC CHALCONE DERIVATIVE INDUCE APOPTOSIS AND SUPPRESS PROINFLAMMATORY CYTOKINES AND CHEMOKINES IN INTERLEUKIN-1β STIMULATED HUMAN THP-1 DERIVED MACROPHAGES","authors":"Chitra Selvarajan, Nalini Ganesan","doi":"10.22159/ijpps.2024v16i3.50391","DOIUrl":"https://doi.org/10.22159/ijpps.2024v16i3.50391","url":null,"abstract":"Objective: Chalcones and their derivatives display a wide range of pharmacological activities. This study examined the effects of AM114, a boronic-chalcone derivative, on human THP-1-derived macrophages with and without interleukin-1β (IL-1β) stimulation. \u0000Methods: AM114 and Aspirin-treated THP-1-derived macrophages underwent activation with or without interleukin-1β. The IC50 concentrations of AM114 and Aspirin were determined through an MTT test. Apoptosis was measured using various techniques, including staining with acridine orange/Ethidium bromide, Hoechst 33342, and rhodamine 123 assays. Caspase-3 activity was measured using the spectrofluorimetric technique, while DNA fragmentation was assessed via agarose gel electrophoresis. Pro-inflammatory cytokines such as interleukin-6 (IL-6) and chemokines like interleukin-8 (IL-8) were measured using enzyme-linked immunosorbent assays.\u0000Results: AM114 and Aspirin showed dose-dependent cytotoxic effects on THP-1 macrophages. Induction of apoptosis was detected in AM114-treated THP-1 macrophages activated with IL-1β compared to macrophages without IL-1β. The gradation of dye uptake, membrane blebbing, increased caspase-3 activity, and DNA fragmentation ensures the induction of apoptosis, which indicates the cell's morphological changes, biochemical processes, and mitochondrial activity. Treating AM114 in IL-1β-activated THP-1 macrophages significantly reduced pro-inflammatory cytokines (IL-6) and chemokines (IL-8), suggesting its anti-cytokine potential in inflammatory diseases.\u0000Conclusion: The study results emphasize that AM114 could act as an anti-inflammatory agent by triggering apoptosis and reducing the release of cytokines and chemokines in inflammatory conditions. As a result, it may be used as a therapeutic option for inflammatory diseases.","PeriodicalId":14188,"journal":{"name":"International Journal of Pharmacy and Pharmaceutical Sciences","volume":"111 37","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140089561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-01DOI: 10.22159/ijpps.2024v16i2.49960
Vishal Bodke, Bharat Tekade, Ruchita Badekar, Swapnil D. Phalak, Mohan Kale
Oral pulsatile drug delivery systems (PDDS) are intended to induce programmable lag phases before a quick and quantifiable, repeated, or prolonged medication release. As a result, they are gaining popularity due to their inherent suitability for achieving chronotherapeutic goals, which have just been highlighted concerning several prevalent chronic illnesses characterized by typical night or early-morning recurring symptoms (e. g. bronchial asthma, heart attack, rheumatoid arthritis, early-morningawakening). Furthermore, time-based colonic release is possible when pulsatile delivery devices are correctly modified to overcome unexpected gastric emptying and give delay periods that roughly match the small intestine transit time. Oral pulsatile administration is accomplished using several release platforms, including reservoir, capsular, and osmotic devices. The current review article addressed the topics that followed: the reason pulsatile drug delivery systems have been invented; diseases for which pulsatile release is necessary; classification, advantages and disadvantages; methods used in the current systems; the situation nowadays and its potential for the future; recent advancements, and especially, the previous five to ten years of research on pulsatile drug delivery conducted by researchers using a variety of drugs for a variety of diseases.
{"title":"PULSATILE DRUG DELIVERY SYSTEMS THE NOVEL APPROACH","authors":"Vishal Bodke, Bharat Tekade, Ruchita Badekar, Swapnil D. Phalak, Mohan Kale","doi":"10.22159/ijpps.2024v16i2.49960","DOIUrl":"https://doi.org/10.22159/ijpps.2024v16i2.49960","url":null,"abstract":"Oral pulsatile drug delivery systems (PDDS) are intended to induce programmable lag phases before a quick and quantifiable, repeated, or prolonged medication release. As a result, they are gaining popularity due to their inherent suitability for achieving chronotherapeutic goals, which have just been highlighted concerning several prevalent chronic illnesses characterized by typical night or early-morning recurring symptoms (e. g. bronchial asthma, heart attack, rheumatoid arthritis, early-morningawakening). Furthermore, time-based colonic release is possible when pulsatile delivery devices are correctly modified to overcome unexpected gastric emptying and give delay periods that roughly match the small intestine transit time. Oral pulsatile administration is accomplished using several release platforms, including reservoir, capsular, and osmotic devices.\u0000The current review article addressed the topics that followed: the reason pulsatile drug delivery systems have been invented; diseases for which pulsatile release is necessary; classification, advantages and disadvantages; methods used in the current systems; the situation nowadays and its potential for the future; recent advancements, and especially, the previous five to ten years of research on pulsatile drug delivery conducted by researchers using a variety of drugs for a variety of diseases.","PeriodicalId":14188,"journal":{"name":"International Journal of Pharmacy and Pharmaceutical Sciences","volume":"33 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139876244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-01DOI: 10.22159/ijpps.2024v16i2.49824
V. Kashyap, Pragya Srivastava, Hedaytullah, Shadab Alam
Objective: The goal of this study aimed to evaluate the protective and vascular effect of the polyherbal trikatu in rats on isoproterenol (ISO) triggered myocardial infarction (MI). Methods: For a total of two days in a row at 24 h breaks (27th and 28th d), a subcutaneous (s.c.) injection of isoproterenol (85 mg/kg body weight) was used to induce myocardial infarction. The rats in Group I behaved as the normal control without pretreatment. Rats in Group II were given isoproterenol. The rats in Group III were selected as the standard, treated with vitamin E (10 mg/kg, p.o.) for 28 d and subjected to isoproterenol (ISO) toxicity. Rats of Group IV and Group V received test sample trikatu 100 mg/kg and 200 mg/kg, respectively for 28 d and were subjected to isoproterenol (ISO) toxicity. Results: Rats given isoproterenol treatment revealed a considerable elevation of serum enzyme cardiac troponin I (cTnI), aspartate aminotransferase (AST), alanine aminotransferase (ALT), Heart creatinine kinase (CK-MB), Lactase dehydrogenase (LDH). Rats pretreated with trikatu and vitamin E+ISO showed significant different (p<0.001) for AST, ALT, LDH and CK-MB levels elevated by ISO. Histopathological tests showed that trikatu and vitamin E decreased inflammation and edema in the hearts of rats. Conclusion: The aqueous suspension of trikatu churna was found to be significantly helpful in minimizing the magnitude of myocardial damage and combating oxidative stress.
研究目的本研究旨在评估多草本植物三卡图对大鼠异丙肾上腺素（ISO）引发的心肌梗死（MI）的保护作用和血管效应：方法：连续两天（第 27 天和第 28 天）间隔 24 小时皮下注射异丙肾上腺素（85 毫克/千克体重）诱发心肌梗死。I 组大鼠作为正常对照，不进行预处理。给 II 组大鼠注射异丙肾上腺素。第三组大鼠被选为标准组，服用维生素 E（10 毫克/千克，口服）28 天，并接受异丙肾上腺素（ISO）毒性试验。第四组和第五组的大鼠分别接受了 100 毫克/千克和 200 毫克/千克的测试样本三卡图治疗 28 天，并接受了异丙肾上腺素（ISO）毒性测试：结果：接受异丙肾上腺素治疗的大鼠血清肌钙蛋白 I（cTnI）、天门冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）、心肌肌酸激酶（CK-MB）和乳酸脱氢酶（LDH）显著升高。使用曲卡图和维生素 E+ISO 预处理的大鼠，其谷丙转氨酶（AST）、谷草转氨酶（ALT）、肌酸激酶（LDH）和肌酸激酶（CK-MB）水平与 ISO 升高的水平有显著差异（p<0.001）。组织病理学测试表明，曲卡图和维生素 E 能减轻大鼠心脏的炎症和水肿：结论：研究发现，三卡图茶（trikatu churna）水悬浮液在最大程度地降低心肌损伤程度和对抗氧化应激方面有明显的帮助。
{"title":"CARDIOPROTECTIVE EFFECT OF TRIKATU CHURNA ON ISOPROTERENOL-INDUCED MYOCARDIAL INFARCTION","authors":"V. Kashyap, Pragya Srivastava, Hedaytullah, Shadab Alam","doi":"10.22159/ijpps.2024v16i2.49824","DOIUrl":"https://doi.org/10.22159/ijpps.2024v16i2.49824","url":null,"abstract":"Objective: The goal of this study aimed to evaluate the protective and vascular effect of the polyherbal trikatu in rats on isoproterenol (ISO) triggered myocardial infarction (MI).\u0000Methods: For a total of two days in a row at 24 h breaks (27th and 28th d), a subcutaneous (s.c.) injection of isoproterenol (85 mg/kg body weight) was used to induce myocardial infarction. The rats in Group I behaved as the normal control without pretreatment. Rats in Group II were given isoproterenol. The rats in Group III were selected as the standard, treated with vitamin E (10 mg/kg, p.o.) for 28 d and subjected to isoproterenol (ISO) toxicity. Rats of Group IV and Group V received test sample trikatu 100 mg/kg and 200 mg/kg, respectively for 28 d and were subjected to isoproterenol (ISO) toxicity.\u0000Results: Rats given isoproterenol treatment revealed a considerable elevation of serum enzyme cardiac troponin I (cTnI), aspartate aminotransferase (AST), alanine aminotransferase (ALT), Heart creatinine kinase (CK-MB), Lactase dehydrogenase (LDH). Rats pretreated with trikatu and vitamin E+ISO showed significant different (p<0.001) for AST, ALT, LDH and CK-MB levels elevated by ISO. Histopathological tests showed that trikatu and vitamin E decreased inflammation and edema in the hearts of rats.\u0000Conclusion: The aqueous suspension of trikatu churna was found to be significantly helpful in minimizing the magnitude of myocardial damage and combating oxidative stress.","PeriodicalId":14188,"journal":{"name":"International Journal of Pharmacy and Pharmaceutical Sciences","volume":"244 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139884027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-01DOI: 10.22159/ijpps.2024v16i2.49824
V. Kashyap, Pragya Srivastava, Hedaytullah, Shadab Alam
Objective: The goal of this study aimed to evaluate the protective and vascular effect of the polyherbal trikatu in rats on isoproterenol (ISO) triggered myocardial infarction (MI). Methods: For a total of two days in a row at 24 h breaks (27th and 28th d), a subcutaneous (s.c.) injection of isoproterenol (85 mg/kg body weight) was used to induce myocardial infarction. The rats in Group I behaved as the normal control without pretreatment. Rats in Group II were given isoproterenol. The rats in Group III were selected as the standard, treated with vitamin E (10 mg/kg, p.o.) for 28 d and subjected to isoproterenol (ISO) toxicity. Rats of Group IV and Group V received test sample trikatu 100 mg/kg and 200 mg/kg, respectively for 28 d and were subjected to isoproterenol (ISO) toxicity. Results: Rats given isoproterenol treatment revealed a considerable elevation of serum enzyme cardiac troponin I (cTnI), aspartate aminotransferase (AST), alanine aminotransferase (ALT), Heart creatinine kinase (CK-MB), Lactase dehydrogenase (LDH). Rats pretreated with trikatu and vitamin E+ISO showed significant different (p<0.001) for AST, ALT, LDH and CK-MB levels elevated by ISO. Histopathological tests showed that trikatu and vitamin E decreased inflammation and edema in the hearts of rats. Conclusion: The aqueous suspension of trikatu churna was found to be significantly helpful in minimizing the magnitude of myocardial damage and combating oxidative stress.
研究目的本研究旨在评估多草本植物三卡图对大鼠异丙肾上腺素（ISO）引发的心肌梗死（MI）的保护作用和血管效应：方法：连续两天（第 27 天和第 28 天）间隔 24 小时皮下注射异丙肾上腺素（85 毫克/千克体重）诱发心肌梗死。I 组大鼠作为正常对照，不进行预处理。给 II 组大鼠注射异丙肾上腺素。第三组大鼠被选为标准组，服用维生素 E（10 毫克/千克，口服）28 天，并接受异丙肾上腺素（ISO）毒性试验。第四组和第五组的大鼠分别接受了 100 毫克/千克和 200 毫克/千克的测试样本三卡图治疗 28 天，并接受了异丙肾上腺素（ISO）毒性测试：结果：接受异丙肾上腺素治疗的大鼠血清肌钙蛋白 I（cTnI）、天门冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）、心肌肌酸激酶（CK-MB）和乳酸脱氢酶（LDH）显著升高。使用曲卡图和维生素 E+ISO 预处理的大鼠，其谷丙转氨酶（AST）、谷草转氨酶（ALT）、肌酸激酶（LDH）和肌酸激酶（CK-MB）水平与 ISO 升高的水平有显著差异（p<0.001）。组织病理学测试表明，曲卡图和维生素 E 能减轻大鼠心脏的炎症和水肿：结论：研究发现，三卡图茶（trikatu churna）水悬浮液在最大程度地降低心肌损伤程度和对抗氧化应激方面有明显的帮助。
{"title":"CARDIOPROTECTIVE EFFECT OF TRIKATU CHURNA ON ISOPROTERENOL-INDUCED MYOCARDIAL INFARCTION","authors":"V. Kashyap, Pragya Srivastava, Hedaytullah, Shadab Alam","doi":"10.22159/ijpps.2024v16i2.49824","DOIUrl":"https://doi.org/10.22159/ijpps.2024v16i2.49824","url":null,"abstract":"Objective: The goal of this study aimed to evaluate the protective and vascular effect of the polyherbal trikatu in rats on isoproterenol (ISO) triggered myocardial infarction (MI).\u0000Methods: For a total of two days in a row at 24 h breaks (27th and 28th d), a subcutaneous (s.c.) injection of isoproterenol (85 mg/kg body weight) was used to induce myocardial infarction. The rats in Group I behaved as the normal control without pretreatment. Rats in Group II were given isoproterenol. The rats in Group III were selected as the standard, treated with vitamin E (10 mg/kg, p.o.) for 28 d and subjected to isoproterenol (ISO) toxicity. Rats of Group IV and Group V received test sample trikatu 100 mg/kg and 200 mg/kg, respectively for 28 d and were subjected to isoproterenol (ISO) toxicity.\u0000Results: Rats given isoproterenol treatment revealed a considerable elevation of serum enzyme cardiac troponin I (cTnI), aspartate aminotransferase (AST), alanine aminotransferase (ALT), Heart creatinine kinase (CK-MB), Lactase dehydrogenase (LDH). Rats pretreated with trikatu and vitamin E+ISO showed significant different (p<0.001) for AST, ALT, LDH and CK-MB levels elevated by ISO. Histopathological tests showed that trikatu and vitamin E decreased inflammation and edema in the hearts of rats.\u0000Conclusion: The aqueous suspension of trikatu churna was found to be significantly helpful in minimizing the magnitude of myocardial damage and combating oxidative stress.","PeriodicalId":14188,"journal":{"name":"International Journal of Pharmacy and Pharmaceutical Sciences","volume":"357 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139824325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-01DOI: 10.22159/ijpps.2024v16i2.49493
Divya Tandon
Objective: The objective of the work is to study the association between Resistin Gene polymorphisms and susceptibility of Oral Squamous Cell carcinoma. Methods: In the present study, we scrutinize the connection between four genetic polymorphisms present in RETN gene with the susceptibility, progression, and clinical outcome of OSCC among 200 OSCC patients and 200 healthy controls. Results: The results of the study reveal that among 260 smokers, the risk of developing OSCC is significantly more among the subjects having history of using betel quid in comparison to those who are not habitual users of betel quid. Conclusion: The study reveals that patients with OSCC exhibiting G/A heterozygous genotype of RETN rs3219175 polymorphism have lesser risk for developing high-grade tumor compared to the patients with G/G homozygotes in North Indian population.
{"title":"RESISTIN GENE POLYMORPHISMS: POTENTIAL BIOMARKER FOR ORAL SQUAMOUS CELL CARCINOMA","authors":"Divya Tandon","doi":"10.22159/ijpps.2024v16i2.49493","DOIUrl":"https://doi.org/10.22159/ijpps.2024v16i2.49493","url":null,"abstract":"Objective: The objective of the work is to study the association between Resistin Gene polymorphisms and susceptibility of Oral Squamous Cell carcinoma.\u0000Methods: In the present study, we scrutinize the connection between four genetic polymorphisms present in RETN gene with the susceptibility, progression, and clinical outcome of OSCC among 200 OSCC patients and 200 healthy controls. \u0000Results: The results of the study reveal that among 260 smokers, the risk of developing OSCC is significantly more among the subjects having history of using betel quid in comparison to those who are not habitual users of betel quid. \u0000Conclusion: The study reveals that patients with OSCC exhibiting G/A heterozygous genotype of RETN rs3219175 polymorphism have lesser risk for developing high-grade tumor compared to the patients with G/G homozygotes in North Indian population.","PeriodicalId":14188,"journal":{"name":"International Journal of Pharmacy and Pharmaceutical Sciences","volume":"30 48","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139684229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-01DOI: 10.22159/ijpps.2024v16i2.49027
Ravi Kant Upadhyay, Simran Sharma
Objective: In this study, albino mice were injected with a sub-lethal dosage of purified wasp Ropalidia Marginata venom toxins to assess the effectiveness of polyclonal anti-venom antibodies. Methods: To neutralize the toxic effects, polyclonal antibodies were generated by immunizing albino mice. The antibody underwent partial purification using ammonium sulphate treatment and octanoic acid precipitation. To detect the presence of antibodies in the antiserum, an immunodouble diffusion test was conducted using Ouchterlony's method (1962). This involved allowing both antigens and antibodies to diffuse radially towards each other from their respective wells. When they reached an equivalence zone, a precipitation complex of antigen and antibody became visible as a concentric band, indicating the development of the combination. To quantitatively determine the amount of antibodies in the antiserum, the equivalency zone approach was used. Results: Experimental mice were injected with a combination containing 400, 800, and 1200 µg of pure antibody, which had been treated serum biomolecules, including metabolic enzymes, completely reversed in the experimental with 40% of the LD50 of wasp venom the elevated serum parameters were glucose, pyruvic acid, lipid, protein and free amino acid, reached to normal (100%) in the treated with 40% of LD50 of the venom and polyclonal treated after 6 h of administration. Anti-serum treatment also successfully normalized the alteration in serum enzyme just after 4h. Similarly, anti-serum treatment also successfully normalized the alteration in serum enzyme just after 4h treated with 40% of LD50 of the venom. Serum ACP content was obtained as 125.35% after 40% of LD50 venom injection, which was get normalized up to 102.81% after 4 h of the anti-venom treatment. Serum ALP content of 114.8% elevation was reversed back to 102.40% after anti-venom treatment. The GPT level significantly reversed up to 102.5%, while it was 130% in the venom-treated mice. A complete reversal was obtained in GPT level, which was obtained as 104.54% in the venom-treated animal. Similarly, LDH which was elevated up to 112.45 % in venom-injected mice was successfully reversed up to 100.25% after anti-venom treatment. Similarly, Ache concentration was fully recovered after anti-venom treatment 6 h, all animals (group B-E) that had received 40% of the LD50 of venom treated with pure antiserum. Conclusion: The venom-injected group showed a complete restoration of serum protein, free amino acid, uric acid, cholesterol, pyruvic acid, total lipid, and glucose level in experimental mice.
{"title":"PRODUCTION OF POLYCLONAL ANTIBODIES AGAINST INDIAN PAPER WASP ROPALIDIA MARGINATA VENOM TOXINS AND THEIR EFFICACY IN THE REVERSAL OF TOXIC EFFECTS","authors":"Ravi Kant Upadhyay, Simran Sharma","doi":"10.22159/ijpps.2024v16i2.49027","DOIUrl":"https://doi.org/10.22159/ijpps.2024v16i2.49027","url":null,"abstract":"Objective: In this study, albino mice were injected with a sub-lethal dosage of purified wasp Ropalidia Marginata venom toxins to assess the effectiveness of polyclonal anti-venom antibodies.\u0000Methods: To neutralize the toxic effects, polyclonal antibodies were generated by immunizing albino mice. The antibody underwent partial purification using ammonium sulphate treatment and octanoic acid precipitation. To detect the presence of antibodies in the antiserum, an immunodouble diffusion test was conducted using Ouchterlony's method (1962). This involved allowing both antigens and antibodies to diffuse radially towards each other from their respective wells. When they reached an equivalence zone, a precipitation complex of antigen and antibody became visible as a concentric band, indicating the development of the combination. To quantitatively determine the amount of antibodies in the antiserum, the equivalency zone approach was used.\u0000Results: Experimental mice were injected with a combination containing 400, 800, and 1200 µg of pure antibody, which had been treated serum biomolecules, including metabolic enzymes, completely reversed in the experimental with 40% of the LD50 of wasp venom the elevated serum parameters were glucose, pyruvic acid, lipid, protein and free amino acid, reached to normal (100%) in the treated with 40% of LD50 of the venom and polyclonal treated after 6 h of administration. Anti-serum treatment also successfully normalized the alteration in serum enzyme just after 4h.\u0000Similarly, anti-serum treatment also successfully normalized the alteration in serum enzyme just after 4h treated with 40% of LD50 of the venom. Serum ACP content was obtained as 125.35% after 40% of LD50 venom injection, which was get normalized up to 102.81% after 4 h of the anti-venom treatment. Serum ALP content of 114.8% elevation was reversed back to 102.40% after anti-venom treatment. The GPT level significantly reversed up to 102.5%, while it was 130% in the venom-treated mice. A complete reversal was obtained in GPT level, which was obtained as 104.54% in the venom-treated animal. Similarly, LDH which was elevated up to 112.45 % in venom-injected mice was successfully reversed up to 100.25% after anti-venom treatment. Similarly, Ache concentration was fully recovered after anti-venom treatment 6 h, all animals (group B-E) that had received 40% of the LD50 of venom treated with pure antiserum.\u0000Conclusion: The venom-injected group showed a complete restoration of serum protein, free amino acid, uric acid, cholesterol, pyruvic acid, total lipid, and glucose level in experimental mice.","PeriodicalId":14188,"journal":{"name":"International Journal of Pharmacy and Pharmaceutical Sciences","volume":"28 18","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139685137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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