Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954853
F. Ratto, P. Matteini, F. Rossi, R. Pini, S. Centi, F. Fusi
We give new insight into multifunctional nanoparticles with light extinction in the therapeutic window, optical stability even on aggregation, as well as possibility of bio-conjugation. The optical response of these particles rests on gold nanorods, which interact with near infrared (NIR) light via plasmonic oscillations, which is an interface effect. Therefore the optical behavior of these particles is challenged by factors which typically occur in the biological sample and under excitation, such as aggregation (e.g. inside endocytic vesicles) and shape transformations. Here silanization of the gold nanorods is proposed as one effective solution to overcome these issues. A shell of porous silica confers isolation from the local environment and additional stability, and also proves suitable for PEGylation and bio-conjugation with e.g. biological macromolecules. We engineer models of aggregation of these particles, in order to investigate its principal effect on their optical response. While in the absence of silica gold nanorods undergo substantial degradation of their plasmonic bands, silanization proves excellent to maintain pristine optical properties even after critical flocculation.
{"title":"Fabrication and optical stability from silanized gold nanorods as transducers of near infrared light","authors":"F. Ratto, P. Matteini, F. Rossi, R. Pini, S. Centi, F. Fusi","doi":"10.1109/IWBP.2011.5954853","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954853","url":null,"abstract":"We give new insight into multifunctional nanoparticles with light extinction in the therapeutic window, optical stability even on aggregation, as well as possibility of bio-conjugation. The optical response of these particles rests on gold nanorods, which interact with near infrared (NIR) light via plasmonic oscillations, which is an interface effect. Therefore the optical behavior of these particles is challenged by factors which typically occur in the biological sample and under excitation, such as aggregation (e.g. inside endocytic vesicles) and shape transformations. Here silanization of the gold nanorods is proposed as one effective solution to overcome these issues. A shell of porous silica confers isolation from the local environment and additional stability, and also proves suitable for PEGylation and bio-conjugation with e.g. biological macromolecules. We engineer models of aggregation of these particles, in order to investigate its principal effect on their optical response. While in the absence of silica gold nanorods undergo substantial degradation of their plasmonic bands, silanization proves excellent to maintain pristine optical properties even after critical flocculation.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"159 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124715044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954835
M. Norgia, A. Pesatori, I. Milesi, R. Dellacà
This work describes the design and realization of an optical interferometer, aimed at the measurement of forced oscillations on the human respiratory system. For the detection of very low signal, a dedicated algorithm was implemented.
{"title":"Self-mixing interferometer for direct vibration measurement on human skin","authors":"M. Norgia, A. Pesatori, I. Milesi, R. Dellacà","doi":"10.1109/IWBP.2011.5954835","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954835","url":null,"abstract":"This work describes the design and realization of an optical interferometer, aimed at the measurement of forced oscillations on the human respiratory system. For the detection of very low signal, a dedicated algorithm was implemented.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126170481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954860
E. Orabona, I. Rea, I. Rendina, L. de Stefano
In this paper we present a numerical model for the design of microfluidic assisted porous silicon microarray for the optical monitoring of biomolecular interactions. The kinetic binding is studied as a function of several physical, chemical and geometrical parameters. Optimization conditions in order to maximize the sensor response and increase the accuracy are given.
{"title":"Modelling biochemical interactions in a microfluidic assisted porous silicon microarray for optical sensing","authors":"E. Orabona, I. Rea, I. Rendina, L. de Stefano","doi":"10.1109/IWBP.2011.5954860","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954860","url":null,"abstract":"In this paper we present a numerical model for the design of microfluidic assisted porous silicon microarray for the optical monitoring of biomolecular interactions. The kinetic binding is studied as a function of several physical, chemical and geometrical parameters. Optimization conditions in order to maximize the sensor response and increase the accuracy are given.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130693903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954863
S. Coppola, V. Vespini, S. Grilli, M. Paturzo, P. Ferraro, L. De Petrocellis, P. Orlando
Nano- and pico-droplets have been extracted and dispensed from sessile drop or liquid film reservoirs through pyroelectric effect activated by a hot tip or an IR laser source on polar dielectric substrates. The manipulation of liquids in 2D and 3D was demonstrated without the need of high-voltage power supplies and/or circuits.
{"title":"Dispensing and manipulation of nano-drops by pyro-EHD (electro-hydro-dynamic) effect","authors":"S. Coppola, V. Vespini, S. Grilli, M. Paturzo, P. Ferraro, L. De Petrocellis, P. Orlando","doi":"10.1109/IWBP.2011.5954863","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954863","url":null,"abstract":"Nano- and pico-droplets have been extracted and dispensed from sessile drop or liquid film reservoirs through pyroelectric effect activated by a hot tip or an IR laser source on polar dielectric substrates. The manipulation of liquids in 2D and 3D was demonstrated without the need of high-voltage power supplies and/or circuits.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"14 4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132568792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954821
A. Duval, M. Nakkach, A. Bellemain, J. Moreau, M. Canva, A. Dhawan, T. Vo‐Dinh
Surface Plasmon Resonance (SPR) sensors are usually based on continuous thin metallic films and coupling of propagative plasmon (PP) modes using high index prism or grating. The quantitative monitoring of the surface evolution is performed by measuring either shift in angular or spectral dip position, or by reflectivity variation in the slope of the angulo-spectral coupling curve. Such sensors are studied in many laboratories and used in a growing number of commercial devices. It appears however that these systems are reaching their limits in terms of sensitivity [1]. In the field of plasmonic sensing, many other works are devoted to the study of metallic nanoparticles. We anticipate that using nanostructured substrates for exciting coupled localized plasmon (CLP) or quasi propagating plasmon (QPP) will allow to further increase the sensors capabilities, in particular in terms of sensitivity. Samples were realized using either e-beam or deep-UV lithography based techniques. Experimental characterizations were conducted using lab-made angulo-spectral SPR Imaging systems [2]. Using RCWA simulations, we have calculated the plasmonics responses, in term of angulo-spectral reflectivity, of nanostructures. A novel narrow groove plasmonic nano-grating sensing configuration exhibits the highest potential [3].
{"title":"Nanostructured substrates for surface plasmon resonance sensors","authors":"A. Duval, M. Nakkach, A. Bellemain, J. Moreau, M. Canva, A. Dhawan, T. Vo‐Dinh","doi":"10.1109/IWBP.2011.5954821","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954821","url":null,"abstract":"Surface Plasmon Resonance (SPR) sensors are usually based on continuous thin metallic films and coupling of propagative plasmon (PP) modes using high index prism or grating. The quantitative monitoring of the surface evolution is performed by measuring either shift in angular or spectral dip position, or by reflectivity variation in the slope of the angulo-spectral coupling curve. Such sensors are studied in many laboratories and used in a growing number of commercial devices. It appears however that these systems are reaching their limits in terms of sensitivity [1]. In the field of plasmonic sensing, many other works are devoted to the study of metallic nanoparticles. We anticipate that using nanostructured substrates for exciting coupled localized plasmon (CLP) or quasi propagating plasmon (QPP) will allow to further increase the sensors capabilities, in particular in terms of sensitivity. Samples were realized using either e-beam or deep-UV lithography based techniques. Experimental characterizations were conducted using lab-made angulo-spectral SPR Imaging systems [2]. Using RCWA simulations, we have calculated the plasmonics responses, in term of angulo-spectral reflectivity, of nanostructures. A novel narrow groove plasmonic nano-grating sensing configuration exhibits the highest potential [3].","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132843272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954828
R. Risi, L. Manti, G. Perna, M. Lasalvia, V. Capozzi, M. Lepore
A Micro-Raman spectroscopic investigation has been performed on human mammary epithelial cells irradiated with different doses of X-ray. Results show that this experimental approach can detect radiation-induced changes in structure, protein, nucleic acid, lipid, and carbohydrate content. Micro- Raman spectroscopy appears to be a very sensitive technique to detect molecular changes even in single human cells following exposure to ionising radiation.
{"title":"Micro-Raman spectroscopy on human mammary epithelial cells irradiated by different doses of X-Rays","authors":"R. Risi, L. Manti, G. Perna, M. Lasalvia, V. Capozzi, M. Lepore","doi":"10.1109/IWBP.2011.5954828","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954828","url":null,"abstract":"A Micro-Raman spectroscopic investigation has been performed on human mammary epithelial cells irradiated with different doses of X-ray. Results show that this experimental approach can detect radiation-induced changes in structure, protein, nucleic acid, lipid, and carbohydrate content. Micro- Raman spectroscopy appears to be a very sensitive technique to detect molecular changes even in single human cells following exposure to ionising radiation.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123767529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954808
A. Baryshev, K. Kawasaki, M. Inoue
Responses from one-dimensional magnetophotonic crystals (1D MPCs) terminated by gold layers have been studied experimentally. These MPCs supported an optical resonance (optical Tamm state-OTS) originating from periodicity of their lattices and the surface plasmons resonance (SPR) on gold films' interfaces. Parameters of the crystals and experimental conditions were chosen such that OTS and SPR modes were excited simultaneously by light from a selected spectral range. Later was observed in spectra as an overlap of the resonances. Tuning the overlap by a variation in experimental conditions resulted in changes of measured optical and magneto-optical responses from the MPCs.
{"title":"Magnetophotonic crystals with metalized surfaces: an approach to optical biosensors","authors":"A. Baryshev, K. Kawasaki, M. Inoue","doi":"10.1109/IWBP.2011.5954808","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954808","url":null,"abstract":"Responses from one-dimensional magnetophotonic crystals (1D MPCs) terminated by gold layers have been studied experimentally. These MPCs supported an optical resonance (optical Tamm state-OTS) originating from periodicity of their lattices and the surface plasmons resonance (SPR) on gold films' interfaces. Parameters of the crystals and experimental conditions were chosen such that OTS and SPR modes were excited simultaneously by light from a selected spectral range. Later was observed in spectra as an overlap of the resonances. Tuning the overlap by a variation in experimental conditions resulted in changes of measured optical and magneto-optical responses from the MPCs.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"251 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116807066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954795
Yi Wang, W. Knoll, J. Dostálek
In this paper, long range surface plasmons (LRSP) and magnetic iron oxide nanoparticle (MNP) immunoassay are investigated for advancing the sensitivity of surface plasmon resonance (SPR) biosensor technology for the analysis of bacterial pathogens. The MNPs-antibody conjugates served as labels for enhancing the binding-induced refractive index changes and as “vehicles” for rapid delivery of bacteria from a sample solution to the sensor surface by applied magnetic field. A sensor chip for diffraction-coupled long range surface plasmon resonance (LRSPR) was developed and functionalized with capture antibodies. These surface plasmon waves enable improving the resolution of refractive index changes measurements on the surface and probing whole volume of captured bacterial pathogens. The combined LRSPR and MNP assay was applied for detection of Escherichia coli O157:H7. About 3 orders of magnitude improvement of the sensitivity with respect to regular SPR and the analysis time of ∼30 min are demonstrated.
{"title":"Long range surface plasmon resonance bacterial pathogen biosensor with magnetic nanoparticle assay","authors":"Yi Wang, W. Knoll, J. Dostálek","doi":"10.1109/IWBP.2011.5954795","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954795","url":null,"abstract":"In this paper, long range surface plasmons (LRSP) and magnetic iron oxide nanoparticle (MNP) immunoassay are investigated for advancing the sensitivity of surface plasmon resonance (SPR) biosensor technology for the analysis of bacterial pathogens. The MNPs-antibody conjugates served as labels for enhancing the binding-induced refractive index changes and as “vehicles” for rapid delivery of bacteria from a sample solution to the sensor surface by applied magnetic field. A sensor chip for diffraction-coupled long range surface plasmon resonance (LRSPR) was developed and functionalized with capture antibodies. These surface plasmon waves enable improving the resolution of refractive index changes measurements on the surface and probing whole volume of captured bacterial pathogens. The combined LRSPR and MNP assay was applied for detection of Escherichia coli O157:H7. About 3 orders of magnitude improvement of the sensitivity with respect to regular SPR and the analysis time of ∼30 min are demonstrated.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117051669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954797
S. Sekatskii
We present single molecule Fluorescence Resonant Energy Transfer Scanning Near-field Optical Microscope (FRET SNOM) images of CdSe nanocrystal (donor) deposited onto the glass slide surface. Images were obtained using the earlier proposed FRET SNOM probes which are 30 – 100 nm thick polymer layers stained with the acceptor dye molecules coated onto the apex of the standard sharp aperture SNOM tip. An original SNOM construction enabling very fast scanning was used to overcome the photostability problem, so we were able to record first single molecules FRET SNOM images, but the photostability is still not enough for regular informative single molecule FRET SNOM imaging. For this, we are elaborating probes based on ultra photostable NV− color centers in nanodiamond crystals attached to the apex of a standard SNOM probe.
{"title":"Single molecule Fluorescent Resonance Energy Transfer Scanning Near-field Optical Microscopy","authors":"S. Sekatskii","doi":"10.1109/IWBP.2011.5954797","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954797","url":null,"abstract":"We present single molecule Fluorescence Resonant Energy Transfer Scanning Near-field Optical Microscope (FRET SNOM) images of CdSe nanocrystal (donor) deposited onto the glass slide surface. Images were obtained using the earlier proposed FRET SNOM probes which are 30 – 100 nm thick polymer layers stained with the acceptor dye molecules coated onto the apex of the standard sharp aperture SNOM tip. An original SNOM construction enabling very fast scanning was used to overcome the photostability problem, so we were able to record first single molecules FRET SNOM images, but the photostability is still not enough for regular informative single molecule FRET SNOM imaging. For this, we are elaborating probes based on ultra photostable NV− color centers in nanodiamond crystals attached to the apex of a standard SNOM probe.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"147 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117095830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954812
C. Vannahme, M. Schelb, S. Klinkhammer, U. Lemmer, T. Mappes
We present a parallel production process enabling the integration of multiple organic semiconductor laser light sources into a PMMA chip intended for the excitation of fluorescent markers of biological samples.
{"title":"Integrated lasers for polymer based lab-on-a-chip systems","authors":"C. Vannahme, M. Schelb, S. Klinkhammer, U. Lemmer, T. Mappes","doi":"10.1109/IWBP.2011.5954812","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954812","url":null,"abstract":"We present a parallel production process enabling the integration of multiple organic semiconductor laser light sources into a PMMA chip intended for the excitation of fluorescent markers of biological samples.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121662856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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