Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954855
P. Matteini, F. Ratto, F. Rossi, R. Pini, Bruno Tiribilli, G. Giambastiani, L. Luconi, L. Dei, Gabriella Caminati
Gold nanorods (GNRs) show enhanced absorption in the near infrared (NIR) region and can be conveniently employed for selective conversion of light into heat. We have succeeded in fabricating hybrid GNRs-polysaccharides composites in the form of semi-solid formulations, films or hydrogels, with good biocompatibility, enhanced stabilization in physiological environment and under laser irradiation and high versatility. Thanks to these properties, these materials are good candidates for applications in many biomedical methodologies including laser-mediated tissue repair and drug delivery. The present results are encouraging toward the development of a novel minimally-invasive technology based on the introduction of bio-inspired nanoplasmonic materials into photothermal applications.
{"title":"Hybrid laser-activatable gold nanorods-loaded hydrogels for photothermal applications","authors":"P. Matteini, F. Ratto, F. Rossi, R. Pini, Bruno Tiribilli, G. Giambastiani, L. Luconi, L. Dei, Gabriella Caminati","doi":"10.1109/IWBP.2011.5954855","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954855","url":null,"abstract":"Gold nanorods (GNRs) show enhanced absorption in the near infrared (NIR) region and can be conveniently employed for selective conversion of light into heat. We have succeeded in fabricating hybrid GNRs-polysaccharides composites in the form of semi-solid formulations, films or hydrogels, with good biocompatibility, enhanced stabilization in physiological environment and under laser irradiation and high versatility. Thanks to these properties, these materials are good candidates for applications in many biomedical methodologies including laser-mediated tissue repair and drug delivery. The present results are encouraging toward the development of a novel minimally-invasive technology based on the introduction of bio-inspired nanoplasmonic materials into photothermal applications.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"23 1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134320791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954837
J. Kalkman, T. V. van Leeuwen, R. Sprik
We present simulations of the decorrelation rate for a high and a low coherence light source in heterodyne light scattering geometry. Light scatters on an ensemble of particles of which the particle trajectories are calculated using a forced-diffusion model. The low coherence signal is constructed in the Fourier domain (as in Fourier-domain optical coherence tomography).
{"title":"Path-length-resolved forced-diffusive particle dynamics in Fourier-domain optical coherence tomography","authors":"J. Kalkman, T. V. van Leeuwen, R. Sprik","doi":"10.1109/IWBP.2011.5954837","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954837","url":null,"abstract":"We present simulations of the decorrelation rate for a high and a low coherence light source in heterodyne light scattering geometry. Light scatters on an ensemble of particles of which the particle trajectories are calculated using a forced-diffusion model. The low coherence signal is constructed in the Fourier domain (as in Fourier-domain optical coherence tomography).","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130963799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954813
M. Perenzoni, D. Perenzoni, D. Stoppa, V. Mulloni, F. Solazzi, G. Resta, B. Margesin
A low-cost integrated microsensor for biomedical applications is proposed. It is composed by an antenna-coupled microbolometer as detector, which is coupled to a specifically designed integrated circuit for biasing and readout. The main features are fast response to radiation, device level reference sensor, and electronic time-correlation for pulsed sources. The detector and the integrated circuits have been fabricated in a custom MEMS technology and a standard CMOS technology, respectively. First electrical measurements show properly operating devices.
{"title":"Terahertz microsensor for biomedical applications","authors":"M. Perenzoni, D. Perenzoni, D. Stoppa, V. Mulloni, F. Solazzi, G. Resta, B. Margesin","doi":"10.1109/IWBP.2011.5954813","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954813","url":null,"abstract":"A low-cost integrated microsensor for biomedical applications is proposed. It is composed by an antenna-coupled microbolometer as detector, which is coupled to a specifically designed integrated circuit for biasing and readout. The main features are fast response to radiation, device level reference sensor, and electronic time-correlation for pulsed sources. The detector and the integrated circuits have been fabricated in a custom MEMS technology and a standard CMOS technology, respectively. First electrical measurements show properly operating devices.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132076613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954864
L. S. Dolci, M. Mirasoli, Angela Buragina, M. Guardigli, A. Roda, F. Bonvicini, Francesca Di Furio
A diagnostic device suitable for Parvovirus B19 genotyping and quantitative DNA detection was developed, based on hybridization with immobilized oligonucleotide probes and chemiluminescence detection. The microfluidic chamber was directly coupled with a CCD sensor in a “contact” imaging configuration to obtain a portable device suitable for point-of-care applications.
{"title":"Point-of-care Parvovirus B19 detection and genotyping based on microfluidics and chemiluminescence “contact” imaging detection","authors":"L. S. Dolci, M. Mirasoli, Angela Buragina, M. Guardigli, A. Roda, F. Bonvicini, Francesca Di Furio","doi":"10.1109/IWBP.2011.5954864","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954864","url":null,"abstract":"A diagnostic device suitable for Parvovirus B19 genotyping and quantitative DNA detection was developed, based on hybridization with immobilized oligonucleotide probes and chemiluminescence detection. The microfluidic chamber was directly coupled with a CCD sensor in a “contact” imaging configuration to obtain a portable device suitable for point-of-care applications.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"27 6","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114019040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954829
M. Lepore, M. Portaccio, B. D. Ventura, L. Mita, D. G. Mita, C. Camerlingo, I. Delfino
Micro-Raman spectroscopy and interval Partial Least Square (iPLS) multivariate analysis have been used for determining glucose concentration in various commercial sport drinks. By employing a visible excitation light (633 nm), micro-Raman spectra in the 600–1600 cm−1 wavenumber shift region have been recorded, showing well defined and separated vibrational fingerprints of the various contained sugars (glucose, fructose and sucrose). Glucose content was quantified by using the iPLS analysis based on a model built by employing aqueous glucose solutions as reference samples. The estimated glucose concentrations are in good agreement with the values obtained by using a biochemical assay. These results represent a significant step towards the development of a fast, simple, cost-effective Raman-based method for glucose quantification in products of food and beverage industry, alternative to expensive, time-, sample- and chemicals-consuming biochemical assays currently used in production and quality control processes.
{"title":"Determination of glucose content by means of visible micro-Raman spectroscopy and interval partial least square multivariate analysis","authors":"M. Lepore, M. Portaccio, B. D. Ventura, L. Mita, D. G. Mita, C. Camerlingo, I. Delfino","doi":"10.1109/IWBP.2011.5954829","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954829","url":null,"abstract":"Micro-Raman spectroscopy and interval Partial Least Square (iPLS) multivariate analysis have been used for determining glucose concentration in various commercial sport drinks. By employing a visible excitation light (633 nm), micro-Raman spectra in the 600–1600 cm−1 wavenumber shift region have been recorded, showing well defined and separated vibrational fingerprints of the various contained sugars (glucose, fructose and sucrose). Glucose content was quantified by using the iPLS analysis based on a model built by employing aqueous glucose solutions as reference samples. The estimated glucose concentrations are in good agreement with the values obtained by using a biochemical assay. These results represent a significant step towards the development of a fast, simple, cost-effective Raman-based method for glucose quantification in products of food and beverage industry, alternative to expensive, time-, sample- and chemicals-consuming biochemical assays currently used in production and quality control processes.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121195624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954801
Mascaro A.L. Allegra, L. Sacconi, F. Pavone, P. Cesare, Giorgio Grasselli, P. Strata
In the adult nervous system, different population of neurons corresponds to different regenerative behavior. Although previous works show that olivocerebellar fibers are capable of axonal regeneration in a suitable environment as a response to injury [1], we have hitherto no details about the real dynamics of fiber regeneration. We coupled two photon imaging to laser-induced lesions to perform in vivo multiphoton nanosurgery in the CNS of living mice expressing fluorescent proteins to investigate the reparative properties of Climbing Fibers (CFs) in the adult CNS, following the time evolution of this plastic process in vivo. Here we show that sprouting may take place in a murine model in the days that follow a sub-micrometric lesion on the distal portion of the CF. Furthermore this unique model allows, through manipulation of the viral vector, to explore the biochemical mechanisms underlying the reparative process. The great potential of long-term two-photon imaging, coupled to genetic manipulation, opens great opportunities to further investigate the dynamic properties of neurons and their rearrangement following an injury.
{"title":"Laser axotomy on cerebellar climbing fibers","authors":"Mascaro A.L. Allegra, L. Sacconi, F. Pavone, P. Cesare, Giorgio Grasselli, P. Strata","doi":"10.1109/IWBP.2011.5954801","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954801","url":null,"abstract":"In the adult nervous system, different population of neurons corresponds to different regenerative behavior. Although previous works show that olivocerebellar fibers are capable of axonal regeneration in a suitable environment as a response to injury [1], we have hitherto no details about the real dynamics of fiber regeneration. We coupled two photon imaging to laser-induced lesions to perform in vivo multiphoton nanosurgery in the CNS of living mice expressing fluorescent proteins to investigate the reparative properties of Climbing Fibers (CFs) in the adult CNS, following the time evolution of this plastic process in vivo. Here we show that sprouting may take place in a murine model in the days that follow a sub-micrometric lesion on the distal portion of the CF. Furthermore this unique model allows, through manipulation of the viral vector, to explore the biochemical mechanisms underlying the reparative process. The great potential of long-term two-photon imaging, coupled to genetic manipulation, opens great opportunities to further investigate the dynamic properties of neurons and their rearrangement following an injury.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"92 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126833141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954814
N. Santana, C. Nieva, À. Sierra, M. Marro, S. Rao, D. Petrov
The identification of biomarkers in the primary tumor is essential for the stratification of breast cancer patients according to the risk of metastasis progression. To characterize the lipogenic phenotype of breast cancer metastatic cells, the Raman excitation was acquired using an InVia Raman microscope (Renishaw) with a backscattered configuration. The spectroscopic differences between metastatic variants, confirmed by Nile Red staining and fluorescence microscopy, indicate the biomedical application of Raman microspectroscopy to identify the metabolic phenotype of metastatic cells.
{"title":"Raman microspectroscopy is a tool to identify the metastatic ability of breast tumors","authors":"N. Santana, C. Nieva, À. Sierra, M. Marro, S. Rao, D. Petrov","doi":"10.1109/IWBP.2011.5954814","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954814","url":null,"abstract":"The identification of biomarkers in the primary tumor is essential for the stratification of breast cancer patients according to the risk of metastasis progression. To characterize the lipogenic phenotype of breast cancer metastatic cells, the Raman excitation was acquired using an InVia Raman microscope (Renishaw) with a backscattered configuration. The spectroscopic differences between metastatic variants, confirmed by Nile Red staining and fluorescence microscopy, indicate the biomedical application of Raman microspectroscopy to identify the metabolic phenotype of metastatic cells.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115245864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954818
D. Piras, Wenfeng Xia, M. Heijblom, E. M. T. Tije, Johannes C.G. van Hespen, W. Steenbergen, T. G. V. Leeuwen, S. Manohar, F. V. D. Engh, Joost M. Klaase
Breast cancer detection using a parallel plate concfiguration photoacoustic mammoscope is presented. Patients with highly suspicious palpable lesions are included in our clinical protocol. First results from a patient with infiltrative ductal carcinoma are presented.
{"title":"Breast imaging with the Twente Photoacoustic Mammoscope","authors":"D. Piras, Wenfeng Xia, M. Heijblom, E. M. T. Tije, Johannes C.G. van Hespen, W. Steenbergen, T. G. V. Leeuwen, S. Manohar, F. V. D. Engh, Joost M. Klaase","doi":"10.1109/IWBP.2011.5954818","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954818","url":null,"abstract":"Breast cancer detection using a parallel plate concfiguration photoacoustic mammoscope is presented. Patients with highly suspicious palpable lesions are included in our clinical protocol. First results from a patient with infiltrative ductal carcinoma are presented.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"72 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115957157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954810
P. Rivolo, G. Digregorio, F. Frascella, P. Mandracci, M. Ballarini, F. Giorgis, E. Descrovi, L. Dominici, F. Michelotti
We report on time resolved sensing experiments demonstrating the direct, label-free and specific binding of an antibody/antibody system probed by means of Bloch surface waves on silicon-based one-dimensional photonic crystals.
{"title":"Realtime antibody-antibody detection by means of Bloch surface waves on silicon-based multilayers","authors":"P. Rivolo, G. Digregorio, F. Frascella, P. Mandracci, M. Ballarini, F. Giorgis, E. Descrovi, L. Dominici, F. Michelotti","doi":"10.1109/IWBP.2011.5954810","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954810","url":null,"abstract":"We report on time resolved sensing experiments demonstrating the direct, label-free and specific binding of an antibody/antibody system probed by means of Bloch surface waves on silicon-based one-dimensional photonic crystals.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"72 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115718489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954799
F. Merola, S. Coppola, V. Vespini, S. Grilli, P. Ferraro, D. Balduzzi, A. Galli, R. Puglisi
Optical trapping is accomplished by means of polymeric micro-axicons, a sort of conical lenses with great depth of focus. A survey of several applications will be given.
{"title":"Trapping and manipulating micro-objects by bessel beams obtained through polymeric microaxicons","authors":"F. Merola, S. Coppola, V. Vespini, S. Grilli, P. Ferraro, D. Balduzzi, A. Galli, R. Puglisi","doi":"10.1109/IWBP.2011.5954799","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954799","url":null,"abstract":"Optical trapping is accomplished by means of polymeric micro-axicons, a sort of conical lenses with great depth of focus. A survey of several applications will be given.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128547063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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