Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954855
P. Matteini, F. Ratto, F. Rossi, R. Pini, Bruno Tiribilli, G. Giambastiani, L. Luconi, L. Dei, Gabriella Caminati
Gold nanorods (GNRs) show enhanced absorption in the near infrared (NIR) region and can be conveniently employed for selective conversion of light into heat. We have succeeded in fabricating hybrid GNRs-polysaccharides composites in the form of semi-solid formulations, films or hydrogels, with good biocompatibility, enhanced stabilization in physiological environment and under laser irradiation and high versatility. Thanks to these properties, these materials are good candidates for applications in many biomedical methodologies including laser-mediated tissue repair and drug delivery. The present results are encouraging toward the development of a novel minimally-invasive technology based on the introduction of bio-inspired nanoplasmonic materials into photothermal applications.
{"title":"Hybrid laser-activatable gold nanorods-loaded hydrogels for photothermal applications","authors":"P. Matteini, F. Ratto, F. Rossi, R. Pini, Bruno Tiribilli, G. Giambastiani, L. Luconi, L. Dei, Gabriella Caminati","doi":"10.1109/IWBP.2011.5954855","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954855","url":null,"abstract":"Gold nanorods (GNRs) show enhanced absorption in the near infrared (NIR) region and can be conveniently employed for selective conversion of light into heat. We have succeeded in fabricating hybrid GNRs-polysaccharides composites in the form of semi-solid formulations, films or hydrogels, with good biocompatibility, enhanced stabilization in physiological environment and under laser irradiation and high versatility. Thanks to these properties, these materials are good candidates for applications in many biomedical methodologies including laser-mediated tissue repair and drug delivery. The present results are encouraging toward the development of a novel minimally-invasive technology based on the introduction of bio-inspired nanoplasmonic materials into photothermal applications.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"23 1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134320791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954797
S. Sekatskii
We present single molecule Fluorescence Resonant Energy Transfer Scanning Near-field Optical Microscope (FRET SNOM) images of CdSe nanocrystal (donor) deposited onto the glass slide surface. Images were obtained using the earlier proposed FRET SNOM probes which are 30 – 100 nm thick polymer layers stained with the acceptor dye molecules coated onto the apex of the standard sharp aperture SNOM tip. An original SNOM construction enabling very fast scanning was used to overcome the photostability problem, so we were able to record first single molecules FRET SNOM images, but the photostability is still not enough for regular informative single molecule FRET SNOM imaging. For this, we are elaborating probes based on ultra photostable NV− color centers in nanodiamond crystals attached to the apex of a standard SNOM probe.
{"title":"Single molecule Fluorescent Resonance Energy Transfer Scanning Near-field Optical Microscopy","authors":"S. Sekatskii","doi":"10.1109/IWBP.2011.5954797","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954797","url":null,"abstract":"We present single molecule Fluorescence Resonant Energy Transfer Scanning Near-field Optical Microscope (FRET SNOM) images of CdSe nanocrystal (donor) deposited onto the glass slide surface. Images were obtained using the earlier proposed FRET SNOM probes which are 30 – 100 nm thick polymer layers stained with the acceptor dye molecules coated onto the apex of the standard sharp aperture SNOM tip. An original SNOM construction enabling very fast scanning was used to overcome the photostability problem, so we were able to record first single molecules FRET SNOM images, but the photostability is still not enough for regular informative single molecule FRET SNOM imaging. For this, we are elaborating probes based on ultra photostable NV− color centers in nanodiamond crystals attached to the apex of a standard SNOM probe.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"147 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117095830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954813
M. Perenzoni, D. Perenzoni, D. Stoppa, V. Mulloni, F. Solazzi, G. Resta, B. Margesin
A low-cost integrated microsensor for biomedical applications is proposed. It is composed by an antenna-coupled microbolometer as detector, which is coupled to a specifically designed integrated circuit for biasing and readout. The main features are fast response to radiation, device level reference sensor, and electronic time-correlation for pulsed sources. The detector and the integrated circuits have been fabricated in a custom MEMS technology and a standard CMOS technology, respectively. First electrical measurements show properly operating devices.
{"title":"Terahertz microsensor for biomedical applications","authors":"M. Perenzoni, D. Perenzoni, D. Stoppa, V. Mulloni, F. Solazzi, G. Resta, B. Margesin","doi":"10.1109/IWBP.2011.5954813","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954813","url":null,"abstract":"A low-cost integrated microsensor for biomedical applications is proposed. It is composed by an antenna-coupled microbolometer as detector, which is coupled to a specifically designed integrated circuit for biasing and readout. The main features are fast response to radiation, device level reference sensor, and electronic time-correlation for pulsed sources. The detector and the integrated circuits have been fabricated in a custom MEMS technology and a standard CMOS technology, respectively. First electrical measurements show properly operating devices.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132076613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954859
Jasenka Memisevic, L. Shriver-Lake, P. Howell, J. Golden, N. Hashemi, Kirsten B. Jackson, F. Ligler
The utility of pathogen and toxin detection systems depends not only upon sensitivity, specificity and capability for multiplexed recognition, but also on access at the point of need, ease of use, and response time. Combining microfluidics and optical biosensors facilitates miniaturization and automation, while careful design makes it possible to test variable quantities of complex matrices such as food (mL) and clinical samples (µL). We report on progress towards an optofluidic system that combines a rotating magnet trap for automated sample processing and a microflow cytometer capable of 4-color analysis to achieve multi-analyte diagnostics. Sample and antibody-coated magnetic beads are introduced into a moving magnetic field created by magnets rotating in the opposite direction of the flow. The color-coded magnetic beads with captured target remain in suspension while fluorescent tracer reagents are introduced to the flow, thus optimizing the binding kinetics and minimizing aggregation. Magnetic beads with bound target and tracer reagents are released for multiplexed analysis by reversing the direction of spinning magnets.
{"title":"Automated sample processing for flow cytometry","authors":"Jasenka Memisevic, L. Shriver-Lake, P. Howell, J. Golden, N. Hashemi, Kirsten B. Jackson, F. Ligler","doi":"10.1109/IWBP.2011.5954859","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954859","url":null,"abstract":"The utility of pathogen and toxin detection systems depends not only upon sensitivity, specificity and capability for multiplexed recognition, but also on access at the point of need, ease of use, and response time. Combining microfluidics and optical biosensors facilitates miniaturization and automation, while careful design makes it possible to test variable quantities of complex matrices such as food (mL) and clinical samples (µL). We report on progress towards an optofluidic system that combines a rotating magnet trap for automated sample processing and a microflow cytometer capable of 4-color analysis to achieve multi-analyte diagnostics. Sample and antibody-coated magnetic beads are introduced into a moving magnetic field created by magnets rotating in the opposite direction of the flow. The color-coded magnetic beads with captured target remain in suspension while fluorescent tracer reagents are introduced to the flow, thus optimizing the binding kinetics and minimizing aggregation. Magnetic beads with bound target and tracer reagents are released for multiplexed analysis by reversing the direction of spinning magnets.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122781479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954862
S. Surdo, L. Strambini, G. Barillaro, F. Carpignano, S. Merlo
In this work, fabrication and testing of closed-loop opto-fluidic microsystems integrating both optical and fluidic functionalities on the same chip are reported. The microsystems consisted of an electrochemically micromachined silicon substrate, on which high-order vertical silicon/air one dimensional photonic crystals (1D PhCs) were integrated together with fluidic microchannels, bonded to a glass cover, the latter provided with inlet/outlet holes for liquid injection/extraction in/out the PhCs air gaps. An experimental characterization was performed by pumping different liquids (water, ethanol, isopropanol) through the opto-fluidic microsystem and by measuring the reflectivity spectrum in the wavelength range 1.0 – 1.7 µm (at normal incidence) with an inplane fiber optic setup. Experimental results, which well agreed with theoretical predictions, demonstrated the high sensitivity of the opto-fluidic microsystem in measuring small refractive index changes, which was estimated to be 1049 nm/RIU.
{"title":"Silicon micromachined photonic crystal integrated in an opto-fluidic microsystem","authors":"S. Surdo, L. Strambini, G. Barillaro, F. Carpignano, S. Merlo","doi":"10.1109/IWBP.2011.5954862","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954862","url":null,"abstract":"In this work, fabrication and testing of closed-loop opto-fluidic microsystems integrating both optical and fluidic functionalities on the same chip are reported. The microsystems consisted of an electrochemically micromachined silicon substrate, on which high-order vertical silicon/air one dimensional photonic crystals (1D PhCs) were integrated together with fluidic microchannels, bonded to a glass cover, the latter provided with inlet/outlet holes for liquid injection/extraction in/out the PhCs air gaps. An experimental characterization was performed by pumping different liquids (water, ethanol, isopropanol) through the opto-fluidic microsystem and by measuring the reflectivity spectrum in the wavelength range 1.0 – 1.7 µm (at normal incidence) with an inplane fiber optic setup. Experimental results, which well agreed with theoretical predictions, demonstrated the high sensitivity of the opto-fluidic microsystem in measuring small refractive index changes, which was estimated to be 1049 nm/RIU.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129369542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954799
F. Merola, S. Coppola, V. Vespini, S. Grilli, P. Ferraro, D. Balduzzi, A. Galli, R. Puglisi
Optical trapping is accomplished by means of polymeric micro-axicons, a sort of conical lenses with great depth of focus. A survey of several applications will be given.
{"title":"Trapping and manipulating micro-objects by bessel beams obtained through polymeric microaxicons","authors":"F. Merola, S. Coppola, V. Vespini, S. Grilli, P. Ferraro, D. Balduzzi, A. Galli, R. Puglisi","doi":"10.1109/IWBP.2011.5954799","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954799","url":null,"abstract":"Optical trapping is accomplished by means of polymeric micro-axicons, a sort of conical lenses with great depth of focus. A survey of several applications will be given.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128547063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954864
L. S. Dolci, M. Mirasoli, Angela Buragina, M. Guardigli, A. Roda, F. Bonvicini, Francesca Di Furio
A diagnostic device suitable for Parvovirus B19 genotyping and quantitative DNA detection was developed, based on hybridization with immobilized oligonucleotide probes and chemiluminescence detection. The microfluidic chamber was directly coupled with a CCD sensor in a “contact” imaging configuration to obtain a portable device suitable for point-of-care applications.
{"title":"Point-of-care Parvovirus B19 detection and genotyping based on microfluidics and chemiluminescence “contact” imaging detection","authors":"L. S. Dolci, M. Mirasoli, Angela Buragina, M. Guardigli, A. Roda, F. Bonvicini, Francesca Di Furio","doi":"10.1109/IWBP.2011.5954864","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954864","url":null,"abstract":"A diagnostic device suitable for Parvovirus B19 genotyping and quantitative DNA detection was developed, based on hybridization with immobilized oligonucleotide probes and chemiluminescence detection. The microfluidic chamber was directly coupled with a CCD sensor in a “contact” imaging configuration to obtain a portable device suitable for point-of-care applications.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"27 6","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114019040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954844
J. Popp, B. Dietzek, M. Schmitt, P. Rosch, R. Moller, C. Krafft
We will present modern trends in Raman microspectroscopy spanning a multitude of different Raman based technologies from e.g. Raman-microspectroscopy, UV resonance Raman, SERS, towards nonlinear Raman microscopy (CARS) for biomedical diagnosis.
{"title":"Raman spectroscopy - An essential tool for biophotonics","authors":"J. Popp, B. Dietzek, M. Schmitt, P. Rosch, R. Moller, C. Krafft","doi":"10.1109/IWBP.2011.5954844","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954844","url":null,"abstract":"We will present modern trends in Raman microspectroscopy spanning a multitude of different Raman based technologies from e.g. Raman-microspectroscopy, UV resonance Raman, SERS, towards nonlinear Raman microscopy (CARS) for biomedical diagnosis.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117099677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954829
M. Lepore, M. Portaccio, B. D. Ventura, L. Mita, D. G. Mita, C. Camerlingo, I. Delfino
Micro-Raman spectroscopy and interval Partial Least Square (iPLS) multivariate analysis have been used for determining glucose concentration in various commercial sport drinks. By employing a visible excitation light (633 nm), micro-Raman spectra in the 600–1600 cm−1 wavenumber shift region have been recorded, showing well defined and separated vibrational fingerprints of the various contained sugars (glucose, fructose and sucrose). Glucose content was quantified by using the iPLS analysis based on a model built by employing aqueous glucose solutions as reference samples. The estimated glucose concentrations are in good agreement with the values obtained by using a biochemical assay. These results represent a significant step towards the development of a fast, simple, cost-effective Raman-based method for glucose quantification in products of food and beverage industry, alternative to expensive, time-, sample- and chemicals-consuming biochemical assays currently used in production and quality control processes.
{"title":"Determination of glucose content by means of visible micro-Raman spectroscopy and interval partial least square multivariate analysis","authors":"M. Lepore, M. Portaccio, B. D. Ventura, L. Mita, D. G. Mita, C. Camerlingo, I. Delfino","doi":"10.1109/IWBP.2011.5954829","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954829","url":null,"abstract":"Micro-Raman spectroscopy and interval Partial Least Square (iPLS) multivariate analysis have been used for determining glucose concentration in various commercial sport drinks. By employing a visible excitation light (633 nm), micro-Raman spectra in the 600–1600 cm−1 wavenumber shift region have been recorded, showing well defined and separated vibrational fingerprints of the various contained sugars (glucose, fructose and sucrose). Glucose content was quantified by using the iPLS analysis based on a model built by employing aqueous glucose solutions as reference samples. The estimated glucose concentrations are in good agreement with the values obtained by using a biochemical assay. These results represent a significant step towards the development of a fast, simple, cost-effective Raman-based method for glucose quantification in products of food and beverage industry, alternative to expensive, time-, sample- and chemicals-consuming biochemical assays currently used in production and quality control processes.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121195624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2011-06-08DOI: 10.1109/IWBP.2011.5954814
N. Santana, C. Nieva, À. Sierra, M. Marro, S. Rao, D. Petrov
The identification of biomarkers in the primary tumor is essential for the stratification of breast cancer patients according to the risk of metastasis progression. To characterize the lipogenic phenotype of breast cancer metastatic cells, the Raman excitation was acquired using an InVia Raman microscope (Renishaw) with a backscattered configuration. The spectroscopic differences between metastatic variants, confirmed by Nile Red staining and fluorescence microscopy, indicate the biomedical application of Raman microspectroscopy to identify the metabolic phenotype of metastatic cells.
{"title":"Raman microspectroscopy is a tool to identify the metastatic ability of breast tumors","authors":"N. Santana, C. Nieva, À. Sierra, M. Marro, S. Rao, D. Petrov","doi":"10.1109/IWBP.2011.5954814","DOIUrl":"https://doi.org/10.1109/IWBP.2011.5954814","url":null,"abstract":"The identification of biomarkers in the primary tumor is essential for the stratification of breast cancer patients according to the risk of metastasis progression. To characterize the lipogenic phenotype of breast cancer metastatic cells, the Raman excitation was acquired using an InVia Raman microscope (Renishaw) with a backscattered configuration. The spectroscopic differences between metastatic variants, confirmed by Nile Red staining and fluorescence microscopy, indicate the biomedical application of Raman microspectroscopy to identify the metabolic phenotype of metastatic cells.","PeriodicalId":142421,"journal":{"name":"2011 International Workshop on Biophotonics","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2011-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115245864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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