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Functional connectivity of paired default mode network subregions in patients with eye trauma. 眼外伤患者配对默认模式网络亚区的功能连通性。
IF 1.9 4区 医学 Q2 OPHTHALMOLOGY Pub Date : 2024-12-18 eCollection Date: 2024-01-01 DOI: 10.18240/ijo.2024.12.13
Zheng-Mao Xing, Dan Song, Jin-Yu Hu, Xian-Mei Zhou, Xuan Liao, Cheng Chen, Hong Wei, Min Kang, Qian Ling, Liang-Qi He, Zhen-Zhe Liu, Jie Zou, Xu Chen, Zhen-Kai Wu, Yi Shao

Aim: To study functional brain abnormalities in patients with eye trauma (ET) and to discuss the pathophysiological mechanisms of ET.

Methods: Totally 31 ET patients and 31 healthy controls (HCs) were recruited. The age, gender, and educational background characteristics of the two groups were similar. After functional magnetic resonance imaging (fMRI) scanning, the subjects' spontaneous brain activity was evaluated with the functional connectivity (FC) method. Receiver operating characteristic (ROC) curve analysis was used to classify the data. Pearson's correlation analysis was used to explore the relationship between FC values in specific brain regions and clinical behaviors in patients with ET.

Results: Significantly increased FC between several regions was identified including the medial prefrontal cortex (MPFC) and left hippocampus formations (HF), the MPFC and left inferior parietal lobule (IPL), the left IPL and left medial temporal lobe (MTL), the left IPL and right MTL, and the right IPL and left MTL. No decreased region-to-region connectivity was detected in default mode network (DMN) sub-regions in patients with ET. Compared with HCs, ET patients exhibited significantly increased FC between several paired DMN regions, as follows: posterior cingulate cortex (PCC) and right HF (HF.R, t=2.196, P=0.032), right inferior parietal cortices (IPC.R) and left MTL (MTL.L, t=2.243, P=0.029), and right MTL (MTL.R) and HF.R (t=2.236, P=0.029).

Conclusion: FC values in multiple brain regions of ET patients are abnormal, suggesting that these brain regions in ET patients may be dysfunctional, which may help to reveal the pathophysiological mechanisms of ET.

目的:研究眼外伤(ET)患者的脑功能异常,探讨ET的病理生理机制。方法:招募31例ET患者和31例健康对照者。两组患者的年龄、性别、学历特征相似。功能磁共振成像(fMRI)扫描后,用功能连通性(FC)方法评估受试者的自发脑活动。采用受试者工作特征(ROC)曲线分析对数据进行分类。结果:内侧前额叶皮层(MPFC)与左侧海马区(HF)、内侧前额叶皮层与左侧下顶叶区(IPL)、左侧下顶叶区与左侧内侧颞叶区(MTL)、左侧前额叶区与右侧颞叶区(MTL)、左侧前额叶区与右侧颞叶区、右侧颞叶区与左侧颞叶区之间的FC值均显著升高。ET患者的默认模式网络(DMN)亚区未发现区域间连通性下降。与hc相比,ET患者在几个配对的DMN区域之间表现出显著增加的FC,如下:后扣带皮层(PCC)和右侧HF (HF)。R, t=2.196, P=0.032),右侧下顶叶皮质(IPC.R)和左侧颞叶皮质(MTL. R)。L, t=2.243, P=0.029),右MTL (MTL. r)和HF。(t=2.236, P=0.029)。结论:ET患者多脑区FC值异常,提示ET患者可能存在功能障碍,有助于揭示ET的病理生理机制。
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引用次数: 0
Predictive value of CA-153, CA-125 and Apo A for ocular metastasis in menopausal female patients with breast cancer. CA-153、CA-125和载子A对绝经期女性乳腺癌眼转移的预测价值。
IF 1.9 4区 医学 Q2 OPHTHALMOLOGY Pub Date : 2024-12-18 eCollection Date: 2024-01-01 DOI: 10.18240/ijo.2024.12.15
Qun-Ce Liu, Qian Ling, Yan-Mei Zeng, Cheng Chen, Hong Wei, Jin-Yu Hu, Jie Zou, Xiao-Yu Wang, Xu Chen, Yi Shao

Aim: To establish a meaningful standard for diagnosing ocular metastasis (OM) in menopausal breast cancer (BC) women, and explore the relationship between CA-153, CA-125, apolipoprotein A, and OM.

Methods: A total of 1362 menopausal female BC patients with OM volunteered to take part in this study between July 2012 and July 2022. Women with BC who are menopausal were found to have an OM incidence of 1.6%. Furthermore, CA-153, CA-125, and apolipoprotein A (Apo A) all contributed to OM in women with BC who are postmenopausal according to binary logistic regression. Receiver operating curve (ROC) analysis was used to assess the diagnostic value of OM in patients with BC.

Results: Both CA-153 and CA-153+CA-125 showed a higher sensitivity of 95.45%, whereas CA-153+Apo A illustrated the highest specificity of 99.02%. Moreover, CA-153 and CA-153+CA-125 had higher areas under the curve (AUC) of 0.973.

Conclusion: The data indicate that the serum concentrations of CA-153 exhibited the most significant predictors of the diagnosis of OM in menopausal women with BC. The current study researches the utility of risk factors in predicting of OM in menopausal BC women and put forward the latest suggestions on their clinical application.

目的:探讨CA-153、CA-125、载脂蛋白a与绝经期乳腺癌(BC)女性眼转移(OM)的关系,建立有意义的诊断标准。方法:2012年7月至2022年7月期间,共有1362名绝经期女性乳腺癌OM患者自愿参加本研究。绝经期乳腺癌患者的OM发病率为1.6%。此外,根据二元logistic回归,CA-153、CA-125和载脂蛋白A (Apo A)都与绝经后BC患者的OM有关。采用受试者工作曲线(Receiver operating curve, ROC)分析评估OM在BC患者中的诊断价值。结果:CA-153和CA-153+CA-125的敏感性均为95.45%,CA-153+Apo a的特异性最高,为99.02%。CA-153和CA-153+CA-125具有较高的曲线下面积(AUC),为0.973。结论:这些数据表明,血清CA-153浓度是绝经期乳腺癌妇女OM诊断的最重要预测因子。本研究旨在探讨危险因素在绝经期BC妇女OM预测中的作用,并对其临床应用提出最新建议。
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引用次数: 0
Influencing factors of ocular pain in dry eye disease patients at high altitude. 高原干眼病患者眼部疼痛的影响因素。
IF 1.9 4区 医学 Q2 OPHTHALMOLOGY Pub Date : 2024-12-18 eCollection Date: 2024-01-01 DOI: 10.18240/ijo.2024.12.08
Peng-Cheng Zhang, Wen-Shan Jiang, Ya-Zhen Wu, Han-Jing Dai, Dan Wang, Yu-Meng Bao, Hong Yan

Aim: To quantify the severity and frequency of ocular pain in Tibetan plateau patients with dry eye, and to evaluate the related factors affecting ocular pain.

Methods: A retrospective study included 160 cases of dry eye disease (DED) patients who were treated from July 2022 to June 2023. Age, gender, occupation, illness course, anxiety, plateau duration, plateau protection, ocular surface disease index scale (OSDI), break-up time (BUT), Schirmer I test (SIT), conjunctivitis, history of ophthalmic medication, autoimmune disease, the workload of daily near vision range, smoking and overnight stay were obtained via comprehensive ophthalmic assessment, and their duration was followed up. Logistic regression analysis was used to determine the related factors affecting ocular pain.

Results: Totally 77.5% (124/160) of DED patients had ocular pain, of which the severity of ocular pain was mild, moderate, and severe in 30.0%, 36.3%, and 11.3% of patients, respectively. Frequency of ocular pain was reported occasional, half the time, frequent, and persistent pain in 19.4%, 36.9%, 16.9%, and 4.4%. OSDI score was 19.67±5.70 (13 to 36), and the level of pain was lowly correlated with OSDI (rs =0.316, P<0.001). Logistic regression showed that in plateau DED patients, increased anxiety led to increased severity and frequency of ocular pain [odds ratio (OR)=3.662, 5.613, 2.387, and 4.870; all P<0.05], professional eye protection and improvement of daily sleep quantity decreased pain (OR=0.307, 0.572, 0.418, and 0.789; all P<0.05), while smoking and general protection of plateau did not affect the severity and frequency of ocular pain (all P>0.05).

Conclusion: Ocular pain is a common complaint in high-altitude DED patients during the pandemic. Anxiety, eye protection, and adequate sleep during the epidemic period are significantly associated with the severity and frequency of ocular pain in patients with plateau DED, while symptoms of DED have relatively little influence on them.

目的:量化青藏高原干眼症患者眼痛的严重程度和发生频率,探讨影响眼痛的相关因素。方法:对2022年7月至2023年6月接受治疗的160例干眼病(DED)患者进行回顾性研究。通过眼科综合评估获得患者的年龄、性别、职业、病程、焦虑程度、平台持续时间、平台保护、眼表疾病指数量表(OSDI)、分离时间(BUT)、Schirmer I试验(SIT)、结膜炎、眼科用药史、自身免疫性疾病、每日近视距负荷、吸烟情况、住院时间,并进行随访。采用Logistic回归分析确定影响眼痛的相关因素。结果:77.5%(124/160)的DED患者有眼痛,其中轻度、中度和重度分别占30.0%、36.3%和11.3%。偶尔、半次、频繁和持续的眼部疼痛发生率分别为19.4%、36.9%、16.9%和4.4%。OSDI评分为19.67±5.70(13 ~ 36)分,疼痛程度与OSDI评分相关性较低(rs =0.316, PPPP>0.05)。结论:大流行期间,眼部疼痛是高原DED患者的常见主诉。流行期的焦虑、护眼、充足睡眠与高原DED患者眼部疼痛的严重程度和频率显著相关,而DED症状对其影响相对较小。
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引用次数: 0
Biocompatibility of light responsive materials prepared for accommodative intraocular lenses manufacturing. 可调节人工晶状体制备的光反应材料的生物相容性。
IF 1.9 4区 医学 Q2 OPHTHALMOLOGY Pub Date : 2024-12-18 eCollection Date: 2024-01-01 DOI: 10.18240/ijo.2024.12.03
Chen-Zi Liu, Ming-Hao Sun, Wei Dai, Lan-Cao Hao, Si-Quan Zhu

Aim: To investigate the biocompatibility and bacterial adhesion properties of light responsive materials (LRM) and analyze the feasibility and biosafety of employing LRM in the preparation of accommodative intraocular lenses (AIOLs).

Methods: Employing fundamental experimental research techniques, LRM with human lens epithelial cells (hLECs) and human retinal pigment epithelium cells (ARPE-19 cells) were co-cultured. Commercially available intraocular lenses (IOLs) were used as controls to perform cell counting kit-8 (CCK-8), cell staining under varying light intensities, cell adhesion and bacterial adhesion experiments.

Results: LRM exhibited a stronger inhibitory effect on the proliferation of ARPE19 cells than commercially available IOLs when co-cultured with the undiluted extract for 96h (P<0.05). Under other culturing conditions, the effects on the proliferation of hLECs and ARPE-19 cells were not significantly different between the two materials. Under the influence of light irradiation at intensities of 200 and 300 mW/cm2, LRM demonstrated a markedly higher inhibitory effect on the survival of hLECs compared to commercially available IOLs (P<0.0001). They also showed a stronger suppressive effect on the survival rate of ARPE-19 cells, with significant differences observed at 200 mW/cm2 (P<0.001) and extremely significant differences at 300 mW/cm2 (P<0.0001). Additionally, compared to commercially available IOLs, LRM had a higher number of cells adhering to their surface (P<0.05), as well as a significantly greater number of adherent bacterium (P<0.0001).

Conclusion: LRM, characterized by their excellent non-contact tunable deformability and low cytotoxicity to ocular tissues, show considerable potential for use in the fabrication of AIOLs. These materials demonstrate strong cell adhesion; however, during photothermal conversion processes involving shape deformation under various light intensities, the resultant temperature rise may harm surrounding cells. These factors suggest that while the material plays a positive role in reducing the incidence of posterior capsule opacification (PCO), it also poses potential risks for retinal damage. Additionally, the strong bacterial adhesion of these materials indicates an increased risk of endophthalmitis.

目的:研究光响应材料(LRM)的生物相容性和细菌粘附性能,分析光响应材料制备适应性人工晶状体(aiol)的可行性和生物安全性。方法:采用基础实验研究技术,将LRM与人晶状体上皮细胞(hLECs)和人视网膜色素上皮细胞(ARPE-19)共培养。以市售人工晶状体(iol)为对照,进行细胞计数试剂盒-8 (CCK-8)、不同光强下的细胞染色、细胞粘附和细菌粘附实验。结果:LRM与未稀释的提取物共培养96h时,对ARPE19细胞的增殖抑制作用强于市售iol (P2); LRM对hLECs存活的抑制作用明显高于市售iol (P2 (P2 (ppp))。结论:LRM具有良好的非接触可调变形性和对眼部组织的低细胞毒性,在aiol的制造中具有相当大的应用潜力。这些材料具有很强的细胞粘附性;然而,在光热转换过程中,在各种光强下发生形状变形,由此产生的温升可能会损害周围的细胞。这些因素提示,该材料在降低后囊膜混浊(PCO)发生率方面发挥积极作用的同时,也存在视网膜损伤的潜在风险。此外,这些材料的强细菌粘附表明眼内炎的风险增加。
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引用次数: 0
Development and research status of intelligent ophthalmology in China. 中国智能眼科的发展与研究现状。
IF 1.9 4区 医学 Q2 OPHTHALMOLOGY Pub Date : 2024-12-18 eCollection Date: 2024-01-01 DOI: 10.18240/ijo.2024.12.20
Di Gong, Wang-Ting Li, Xiao-Meng Li, Cheng Wan, Yong-Jin Zhou, Shu-Jun Wang, Jian-Tao Wang, Yan-Wu Xu, Shao-Chong Zhang, Wei-Hua Yang

This paper analyzes the current status, technological developments, academic exchange platforms, and future challenges and solutions in the field of intelligent ophthalmology (IO) in China. In terms of technology, significant progress has been made in various areas, including diabetic retinopathy, fundus image analysis, quality assessment of medical artificial intelligence products, clinical research methods, technical evaluation, and industry standards. Researchers continually enhance the safety and standardization of IO technology by formulating a series of clinical application guidelines and standards. The establishment of domestic and international academic exchange platforms provides extensive collaboration opportunities for professionals in various fields, and various academic journals serve as publication platforms for IO research. However, challenges such as technological innovation, data privacy and security, lagging regulations, and talent shortages still pose obstacles to future development. To address these issues, future efforts should focus on strengthening technological research and development, regulatory framework construction, talent cultivation, and increasing patient awareness and acceptance of new technologies. By comprehensively addressing these challenges, IO in China is poised to further lead the industry's development on a global scale, bringing more innovation and convenience to the field of ophthalmic healthcare.

本文分析了中国智能眼科领域的现状、技术发展、学术交流平台以及未来面临的挑战和解决方案。技术方面,在糖尿病视网膜病变、眼底图像分析、医疗人工智能产品质量评估、临床研究方法、技术评价、行业标准等多个领域取得重大进展。研究人员通过制定一系列临床应用指南和标准,不断提高IO技术的安全性和规范性。国内外学术交流平台的建立为各领域专业人士提供了广泛的合作机会,各类学术期刊为IO研究提供了出版平台。然而,技术创新、数据隐私和安全、监管滞后、人才短缺等挑战仍然是未来发展的障碍。为了解决这些问题,未来的工作重点应放在加强技术研发、监管框架建设、人才培养以及提高患者对新技术的认识和接受度等方面。通过全面应对这些挑战,中国IO将在全球范围内进一步引领行业发展,为眼科医疗领域带来更多创新和便利。
{"title":"Development and research status of intelligent ophthalmology in China.","authors":"Di Gong, Wang-Ting Li, Xiao-Meng Li, Cheng Wan, Yong-Jin Zhou, Shu-Jun Wang, Jian-Tao Wang, Yan-Wu Xu, Shao-Chong Zhang, Wei-Hua Yang","doi":"10.18240/ijo.2024.12.20","DOIUrl":"10.18240/ijo.2024.12.20","url":null,"abstract":"<p><p>This paper analyzes the current status, technological developments, academic exchange platforms, and future challenges and solutions in the field of intelligent ophthalmology (IO) in China. In terms of technology, significant progress has been made in various areas, including diabetic retinopathy, fundus image analysis, quality assessment of medical artificial intelligence products, clinical research methods, technical evaluation, and industry standards. Researchers continually enhance the safety and standardization of IO technology by formulating a series of clinical application guidelines and standards. The establishment of domestic and international academic exchange platforms provides extensive collaboration opportunities for professionals in various fields, and various academic journals serve as publication platforms for IO research. However, challenges such as technological innovation, data privacy and security, lagging regulations, and talent shortages still pose obstacles to future development. To address these issues, future efforts should focus on strengthening technological research and development, regulatory framework construction, talent cultivation, and increasing patient awareness and acceptance of new technologies. By comprehensively addressing these challenges, IO in China is poised to further lead the industry's development on a global scale, bringing more innovation and convenience to the field of ophthalmic healthcare.</p>","PeriodicalId":14312,"journal":{"name":"International journal of ophthalmology","volume":"17 12","pages":"2308-2315"},"PeriodicalIF":1.9,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11589450/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142853955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of astigmatism and spherical equivalent correction on contrast sensitivity. 散光和球面等效校正对对比灵敏度的影响。
IF 1.9 4区 医学 Q2 OPHTHALMOLOGY Pub Date : 2024-12-18 eCollection Date: 2024-01-01 DOI: 10.18240/ijo.2024.12.12
Maryam Saffarizadeh, Saeed Rahmani, Alireza Akbarzadeh Baghban, Mohammad Ghassemi-Broumand

Aim: To investigate the effect of astigmatism and spherical equivalent (SE) correction on contrast sensitivity (CS).

Methods: In this cross-sectional study, 103 visually normal subjects aged 18 to 36y with bilateral regular astigmatism in range of 1.00 diopter cylinder (DC) to 4.00 DC and normal best-corrected visual acuity (20/20) were recruited. Binocular CS was assessed by linear sine-wave gratings at 1.5, 3, 6, 12, and 18 cycles per degree (cpd), before correction of astigmatism, after full correction of astigmatism by cylindrical spectacle lenses, and after SE of refractive error. The repeated measures ANOVA and Bonferroni test were used to compare the effects of astigmatism correction on logCS.

Results: Totally 39 patients were male and 64 patients were female with the mean age of 28.25±5.38y. The average degree of astigmatism in right and left eye was 2.03±0.83 and 2.10±0.78, respectively. Increases in uncorrected astigmatic power correlated with decreases in the logCS, especially at high spatial frequencies. A statistically significant difference in logCS was found between these three cases: before correction of astigmatism, after SE of refractive error, and after full correction of astigmatism by cylindrical spectacle lenses at all frequencies (P<0.001), except at 18 cpd. At 18 cpd, there was no statistically significant difference between logCS before and after SE of refractive error (P=1.0). Also, there was no statistically significant difference in mean CS between with-the-rule (WTR) and against-the-rule (ATR) astigmatism, before correction of astigmatism, after correction of astigmatism with cylindrical lenses, and after SE of refractive error.

Conclusion: Binocular astigmatism defocus decreases CS depending on the degree of astigmatism power; correction of this will improve patent's quality of vision. Although high astigmatism refractive error (more than 2.00 DC) that is fully corrected by cylindrical spectacle lenses doesn't increase the CS to the maximum value, especially at higher spatial frequencies (12 and 18). Also SE refractive error effects on improving CS in low astigmatism power (less than 2.00 DC), especially at lower spatial frequencies.

目的:探讨像散和球面等效校正对对比灵敏度的影响。方法:选取18 ~ 36岁视力正常、双侧常规散光1.00 ~ 4.00 DC、最佳矫正视力正常(20/20)的受试者103例。采用线性正弦波光栅在1.5、3、6、12和18周期/度(cpd)下评估双眼CS,分别在散光矫正前、散光完全矫正后和屈光不正SE后进行。采用重复测量方差分析和Bonferroni检验比较散光校正对logCS的影响。结果:男性39例,女性64例,平均年龄28.25±5.38岁。左、右眼平均散光度分别为2.03±0.83、2.10±0.78。未校正的像散功率的增加与logCS的降低相关,特别是在高空间频率下。三种情况下,散光矫正前、屈光不正SE、全频率柱形镜片散光矫正后的logCS差异均有统计学意义(PP=1.0)。顺规散光(WTR)与反规散光(ATR)、散光矫正前、柱面透镜散光矫正后、屈光不正SE后的平均CS差异均无统计学意义。结论:双眼散光离焦使CS随散光度数的增加而降低;对其进行矫正将提高患者的视觉质量。虽然高散光屈光不正(超过2.00 DC)被圆柱形眼镜镜片完全矫正后,CS并没有增加到最大值,特别是在较高的空间频率(12和18)。在低散光功率下(小于2.00 DC),特别是在较低的空间频率下,SE屈光不正对CS的改善也有影响。
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引用次数: 0
Short-term effectiveness of intelligent navigated laser photocoagulation versus subthreshold micropulse laser in patients with chronic central serous chorioretinopathy. 智能导航激光光凝术与阈下微脉冲激光对慢性中央浆液性脉络膜视网膜病变患者的短期疗效对比。
IF 1.9 4区 医学 Q2 OPHTHALMOLOGY Pub Date : 2024-11-18 eCollection Date: 2024-01-01 DOI: 10.18240/ijo.2024.11.10
Fen Zhou, Cheng-Hu Wang, Chen-Chen Zhou, Sha Liu, Jin Yao, Qin Jiang

Aim: To compare the short-term effectiveness of intelligent navigated laser photocoagulation and 577-nm subthreshold micropulse laser (SML) treatment in patients with chronic central serous chorioretinopathy (cCSC).

Methods: This observational retrospective cohort study included 60 consecutive patients who underwent intelligent navigated laser photocoagulation (n=30) or 577-nm SML treatment (n=30) for cCSC between Jan. 2021 and Oct. 2022. During 3mo follow-up, all patients underwent assessments of best correct visual acuity (BCVA) and optical coherence tomography (OCT).

Results: The operation of laser treatment was successful in all cases. At 1mo, BCVA improved significantly more in the intelligent navigated laser photocoagulation group compared to the SML group (P<0.05). The change was not significantly different at 3mo (P>0.05). Central macular thickness (CMT) in the intelligent navigated laser photocoagulation group was lower than in the SML group at 1mo (P<0.05). The subfoveal choroidal thickness (SFCT) in two groups were all significantly improved at 3mo (all P<0.05). The change between two groups was not significantly different at 1mo or at 3mo (P>0.05).

Conclusion: Intelligent navigated laser photocoagulation is superior to SML for treating cCSC, leading to better improvements in vision and CMT for short term.

目的:比较智能导航激光光凝和577纳米阈下微脉冲激光(SML)治疗慢性中心性浆液性脉络膜视网膜病变(cCSC)患者的短期疗效:这项观察性回顾性队列研究纳入了2021年1月至2022年10月期间接受智能导航激光光凝术(30例)或577纳米SML治疗(30例)的60例慢性中心性浆液性脉络膜视网膜病变患者。随访3个月期间,所有患者均接受了最佳矫正视力(BCVA)和光学相干断层扫描(OCT)评估:结果:所有病例的激光治疗都很成功。1个月后,智能导航激光光凝组的BCVA改善程度明显高于SML组(PP>0.05)。1个月后,智能导航激光光凝组的黄斑中心厚度(CMT)低于SML组(PPP>0.05):结论:智能导航激光光凝在治疗 cCSC 方面优于 SML,可在短期内更好地改善视力和 CMT。
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引用次数: 0
Cone-rod homeobox transcriptionally activates TCF7 to promote the proliferation of retinal pigment epithelial and retinoblastoma cells in vitro. 锥体-杆状同源染色体转录激活TCF7,促进体外视网膜色素上皮细胞和视网膜母细胞瘤细胞的增殖。
IF 1.9 4区 医学 Q2 OPHTHALMOLOGY Pub Date : 2024-11-18 eCollection Date: 2024-01-01 DOI: 10.18240/ijo.2024.11.04
Na Zhao, Ying-Ying Li, Jia-Man Xu, Mu-Yao Yang, Yun-Zhe Li, Thomas Chuen Lam, Lei Zhou, Qi-Hu Tong, Jun-Tao Zhang, Sheng-Zhan Wang, Xin-Xin Hu, Yu-Fei Wu, Qin-Kang Lu, Ting-Yuan Lang

Aim: To investigate the proliferation regulatory effect of cone-rod homeobox (CRX) in retinal pigment epithelium (RPE) and retinoblastoma (RB) cells to explore the potential application and side effect (oncogenic potential) of CRX-based gene therapy in RPE-based retinopathies.

Methods: Adult human retinal pigment epithelial (ARPE)-19 and human retinal pigment epithelial (RPE)-1 cells and Y79 RB cell were used in the study. Genetic manipulation was performed by lentivirus-based technology. The cell proliferation was determined by a CellTiter-Glo Reagent. The mRNA and protein levels were determined by quantitative real-time polymerase chain reaction (qPCR) and Western blot assay. The transcriptional activity of the promoter was determined by luciferase reporter gene assay. The bindings between CRX and transcription factor 7 (TCF7) promoter as well as TCF7 and the promoters of TCF7 target genes were examined by chromatin immunoprecipitation (ChIP) assay. The transcription of the TCF7 was determined by a modified nuclear run-on assay.

Results: CRX overexpression and knockdown significantly increased (n=3, P<0.05 in all the cells) and decreased (n=3, P<0.01 in all the cells) the proliferation of RPE and RB cells. CRX overexpression and knockdown significantly increased and deceased the mRNA levels of Wnt signaling target genes [including MYC proto-oncogene (MYC), JUN, FOS like 1 (FOSL1), CCND1, cyclin D2 (CCND2), cyclin D3 (CCND3), cellular communication network factor 4 (CCN4), peroxisome proliferator activated receptor delta (PPARD), and matrix metallopeptidase 7 (MMP7)] and the luciferase activity driven by the Wnt signaling transcription factor (TCF7). TCF7 overexpression and knockdown significantly increased and decreased the proliferation of RPE and RB cells and depletion of TCF7 significantly abolished the stimulatory effect of CRX on the proliferation of RPE and RB cells. CRX overexpression and knockdown significantly increased and decreased the mRNA level of TCF7 and the promoter of TCF7 was significantly immunoprecipitated by CRX antibody.

Conclusion: CRX transcriptionally activates TCF7 to promote the proliferation of RPE and RB cells in vitro. CRX is a potential target for RPE-based regenerative medicine. The potential risk of this strategy, tumorigenic potential, should be considered.

目的:研究视锥-视杆细胞同源染色体(CRX)在视网膜色素上皮(RPE)和视网膜母细胞瘤(RB)细胞中的增殖调控作用,以探索基于CRX的基因疗法在基于RPE的视网膜病变中的潜在应用和副作用(致癌潜力):研究使用了成人人视网膜色素上皮(ARPE)-19 和人视网膜色素上皮(RPE)-1 细胞以及 Y79 RB 细胞。基因操作是通过慢病毒技术进行的。细胞增殖由 CellTiter-Glo 试剂测定。mRNA 和蛋白质水平通过实时定量聚合酶链式反应(qPCR)和 Western 印迹检测进行测定。荧光素酶报告基因测定启动子的转录活性。染色质免疫共沉淀(ChIP)检测了 CRX 与转录因子 7(TCF7)启动子的结合,以及 TCF7 与 TCF7 靶基因启动子的结合。TCF7的转录是通过改良的核转录试验确定的:结果:CRX的过表达和敲除显著增加了(n=3,Pn=3,PMYC)、JUN、FOS like 1 (FOSL1)、CCND1、细胞周期蛋白D2 (CCND2)、细胞周期蛋白D3 (CCND3)、细胞通讯网络因子4 (CCN4)、过氧化物酶体增殖激活受体δ (PPARD)和基质金属肽酶7 (MMP7)]以及由Wnt信号转录因子(TCF7)驱动的荧光素酶活性。TCF7的过表达和敲除可显著增加和减少RPE和RB细胞的增殖,而TCF7的耗竭可显著消除CRX对RPE和RB细胞增殖的刺激作用。CRX过表达和敲除可明显提高和降低TCF7的mRNA水平,CRX抗体可明显免疫沉淀TCF7的启动子:结论:CRX可转录激活TCF7,促进体外RPE和RB细胞的增殖。CRX是基于RPE的再生医学的潜在靶点。应考虑到这一策略的潜在风险,即致瘤可能性。
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引用次数: 0
Corneal subepithelial nerve fibers in type 2 diabetes: potential biomarker of diabetic neuropathy. 2 型糖尿病患者的角膜上皮下神经纤维:糖尿病神经病变的潜在生物标记物。
IF 1.9 4区 医学 Q2 OPHTHALMOLOGY Pub Date : 2024-11-18 eCollection Date: 2024-01-01 DOI: 10.18240/ijo.2024.11.12
Ling-Rui Meng, Hua Chen, Wen-Qian Chen, Yi Gao, Zi-Wei Li, Zi Ye, Zhao-Hui Li

Aim: To observe the changes in corneal subepithelial nerve fibers (CNFs) and Langerhans cells (LCs) in patients with type 2 diabetes using corneal laser confocal microscopy (CLCM).

Methods: A total of 60 patients (64 eyes), including 40 patients with type 2 diabetes (DM group) and 20 subjects without diabetes (control group) were included with CLCM. Neuron J plugin of Image J software were used for quantitative analysis of CNF length (CNFL), CNF density (CNFD), corneal nerve branch fiber density (CNBD), main branch length density, branch length density, corneal nerve fiber tortuosity (NT) score, and LCs density. An independent samples t-test to analyze the variability between the two groups was performed, and Pearson correlation analysis was used to analyze the relationships between CNF and multiple biochemical indicators in the DM group. The predictive power of CNF for type 2 diabetes was assessed using the receiver operating characteristic (ROC) curve.

Results: There were significant differences in the CNFL, CNFD, and main branch length density between two groups. The results of Pearson correlation analysis showed a significant negative correlation between CNFD and the duration of diabetes as well as triglyceride levels and total cholesterol, and a significant positive correlation between CNFD and serum albumin. In addition, the NT score showed a positive correlation and urea nitrogen, similar to the positive correlation observed between LC density and glycosylated hemoglobin (HbA1c) levels. CNFD showed the highest area under the curve (AUC of ROC) value, followed by main branch length density and CNFL. The AUC of the ROC curve under the logistic regression model also demonstrated good predictive values. The cut-off values of CNFD, CNFL, and main branch length density for diabetes showed 31.25, 18.85, and 12.56, respectively.

Conclusion: In patients with type 2 diabetes, there is a notable reduction in both CNFL and CNFD. These measurements can be influenced by various blood biochemical factors. However, the compromised nerve fibers can serve as valuable indicators for predicting the onset of type 2 diabetes and also as biomarkers for detecting diabetic neuropathy and its related complications.

目的:使用角膜激光共聚焦显微镜(CLCM)观察2型糖尿病患者角膜上皮下神经纤维(CNFs)和朗格汉斯细胞(LCs)的变化:共有 60 名患者(64 只眼)接受了角膜激光共聚焦显微镜检查,其中包括 40 名 2 型糖尿病患者(DM 组)和 20 名非糖尿病患者(对照组)。使用 Image J 软件的 Neuron J 插件对角膜神经纤维长度(CNFL)、角膜神经纤维密度(CNFD)、角膜神经纤维分支密度(CNBD)、角膜神经纤维主支长度密度、角膜神经纤维分支长度密度、角膜神经纤维迂曲度(NT)评分和角膜神经纤维密度进行定量分析。采用独立样本t检验分析两组间的变异性,并采用皮尔逊相关分析法分析DM组CNF与多项生化指标之间的关系。使用接收者操作特征曲线(ROC)评估了CNF对2型糖尿病的预测能力:结果:两组间的 CNFL、CNFD 和主支长度密度存在明显差异。皮尔逊相关分析结果显示,CNFD与糖尿病病程、甘油三酯水平和总胆固醇水平呈显著负相关,CNFD与血清白蛋白呈显著正相关。此外,NT 评分与尿素氮呈正相关,类似于 LC 密度与糖化血红蛋白(HbA1c)水平之间的正相关。CNFD 的曲线下面积(ROC 的 AUC)值最高,其次是主枝长度密度和 CNFL。逻辑回归模型的 ROC 曲线的 AUC 值也显示出良好的预测价值。CNFD、CNFL和主支长度密度对糖尿病的临界值分别为31.25、18.85和12.56:结论:2 型糖尿病患者的 CNFL 和 CNFD 均明显下降。结论:在 2 型糖尿病患者中,CNFL 和 CNFD 都明显下降,这些测量值可能受到各种血液生化因素的影响。然而,受损的神经纤维可作为预测 2 型糖尿病发病的重要指标,也可作为检测糖尿病神经病变及其相关并发症的生物标志物。
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引用次数: 0
Short-term fluctuation of intraocular pressure and influencing factors following intravitreal injection in patients with retinal vascular diseases. 视网膜血管疾病患者玻璃体内注射后的短期眼压波动及其影响因素。
IF 1.9 4区 医学 Q2 OPHTHALMOLOGY Pub Date : 2024-11-18 eCollection Date: 2024-01-01 DOI: 10.18240/ijo.2024.11.11
Jing-Peng Miao, Yi-Yun Zeng, Xin-Ming Gu, Xin-Yuan Zhang

Aim: To investigate the patterns of short-term intraocular pressure (IOP) fluctuations and identify the contributing factors following intravitreal injection in patients with retinal vascular diseases.

Methods: Totally 81 patients were enrolled in this case control study. Eyes were categorized into 7 groups, including age-related macular degeneration (AMD), polypoidal choroidal vasculopathy (PCV), idiopathic choroidal neovascularization (CNV), proliferative diabetic retinopathy (PDR), diabetic macular edema (DME), macular edema secondary to branch (BVOME) and central (CVOME) retinal vein occlusion. IOP was measured in all patients using rebound tonometer at 7 preset time points perioperatively. Additionally, based on the administered medication, the eyes were classified into three treatment groups, including dexamethasone intravitreal implant (IVO), intravitreal conbercept (IVC), and intravitreal ranibizumab (IVR). To compare IOP values at various time points across groups, we employed one-way ANOVA, independent sample t-test or χ 2 test and multivariate logistic regression analysis.

Results: Peak IOP values across all groups were observed at 40s, and 5min after intravitreal injection. Statistical differences in IOP were detected at the 5min among the 7 indication groups (F=2.50, P=0.029). When examing the impact of medications, the IVO group exhibited lower average IOP values at both 40s and 5min compared to the IVC and IVR groups (P<0.001; P=0.007). The IOP values at 40s and 5min were significantly higher in BVOME and CVOME group compared to non-retinal vein occlusion-secondary macular edema (RVOME) group (P<0.001). Multivariate logistic regression analysis further confirmed that IOP measurement at 40s was significantly higher in CVOME group than in non-RVOME group (OR=1.64, 95%CI: 1.09-2.47; P=0.018).

Conclusion: Needle size plays a crucial role in the transient changes of IOP following intravitreal injection. Before administering intravitreal injection to patients with central retinal vein occlusion, it is essential to exclude any underlysing causes of increased IOP.

目的:研究视网膜血管疾病患者玻璃体内注射后短期内眼压(IOP)波动的模式,并确定其诱因:本病例对照研究共纳入 81 例患者。方法:这项病例对照研究共纳入 81 名患者,将其分为 7 组,包括老年性黄斑变性(AMD)、多形性脉络膜血管病(PCV)、特发性脉络膜新生血管(CNV)、增殖性糖尿病视网膜病变(PDR)、糖尿病性黄斑水肿(DME)、视网膜分支静脉闭塞(BVOME)和视网膜中央静脉闭塞(CVOME)引起的黄斑水肿。在围手术期的 7 个预设时间点,使用回弹式眼压计测量所有患者的眼压。此外,根据用药情况,患者被分为三个治疗组,包括地塞米松玻璃体内植入组(IVO)、康贝赛普玻璃体内植入组(IVC)和雷尼单抗玻璃体内植入组(IVR)。为了比较各组不同时间点的眼压值,我们采用了单因素方差分析、独立样本 t 检验或 χ 2 检验以及多变量逻辑回归分析:所有组的峰值眼压都出现在玻璃体内注射后的 40s 和 5 分钟。7 个适应症组在 5 分钟时的眼压存在统计学差异(F=2.50,P=0.029)。在研究药物的影响时,IVO 组在 40s 和 5min 时的平均眼压值均低于 IVC 组和 IVR 组(PP=0.007)。与非视网膜静脉闭塞-继发性黄斑水肿(RVOME)组相比,BVOME 组和 CVOME 组在 40s 和 5min 时的眼压值明显更高(PP=0.018):针头大小对玻璃体内注射后眼压的短暂变化起着至关重要的作用。在对视网膜中央静脉闭塞患者进行玻璃体内注射前,必须排除导致眼压升高的任何潜在原因。
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引用次数: 0
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International journal of ophthalmology
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