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Larvicidal Activity of a Natural Botanical Biostop Moustiques® andPhysiological Changes Induced in Susceptible and Resistant Strains ofAnopheles gambiae Giles (Diptera: Culicidae) 一种天然植物型灭蚊®的杀幼虫活性及冈比亚按蚊敏感和耐药菌株的生理变化(双翅目:库蚊科)
Pub Date : 2015-02-20 DOI: 10.2174/1874407901509010012
K. M. Ahadji-Dabla, J. Brunet, G. Ketoh, G. Apétogbo, I. Glitho, L. Belzunces
The larvicidal activity of Biostop Moustiques ® (BM), a botanical biocide, was studied on susceptible and resis- tant strains of Anopheles gambiae s.s. at the concentrations of 1, 5, 10 and 20 mL/L of water. In addition to mortality and total protein concentration, the effect of BM on the activity of the following metabolic enzymes was evaluated in fourth instar larvae: glutathione-S-transferase (GST), alkaline phosphatase (ALP), superoxide dismutase (SOD), lactate dehy- drogenase (LDH) and glucose 6-phosphate dehydrogenase (G6PDH). BM caused more than 90% mortality at different larval stages and had median lethal concentration (LC50) of 8.13 mL/L in susceptible Kisumu strain and 8.08 mL/L in re- sistant Acerkis strain. Protein concentration increased significantly in both strains when BM concentration was higher than 1 mL/L. GST and ALP activities increased significantly in both strains. LDH activity increased in Kisumu strain at 5 mL/L and decreased in Acerkis strain at all concentrations. G6PDH activity significantly increased with a maximum ef- fect at 1 mL/L for Acerkis and 5 mL/L for Kisumu. BM completely suppressed SOD activity at 10 mL/L for Kisumu strain and 1 mL/L for Acerkis strain. This study showed that BM had a high larvicidal activity against both strains of Anopheles gambiae and it elicited a wide range of physiological changes.
研究了植物性杀菌剂Biostop Moustiques®(BM)在1、5、10和20 mL/L水浓度下对冈比亚按蚊敏感株和抗性株的杀幼虫活性。除死亡率和总蛋白浓度外,还研究了BM对4龄幼虫谷胱甘肽s转移酶(GST)、碱性磷酸酶(ALP)、超氧化物歧化酶(SOD)、乳酸脱氢酶(LDH)和葡萄糖6-磷酸脱氢酶(G6PDH)活性的影响。BM在不同幼虫期的死亡率均在90%以上,敏感株Kisumu和抗性株Acerkis的中位致死浓度(LC50)分别为8.13 mL/L和8.08 mL/L。当BM浓度高于1 mL/L时,两种菌株的蛋白浓度均显著升高。GST和ALP活性均显著升高。在5 mL/L浓度下,Kisumu菌株的LDH活性升高,Acerkis菌株的LDH活性降低。G6PDH活性显著增加,Acerkis为1 mL/L, Kisumu为5 mL/L时效果最大。在10 mL/L的Kisumu菌株和1 mL/L的Acerkis菌株中,BM完全抑制了SOD活性。本研究表明,BM对两种冈比亚按蚊均有较高的杀幼虫活性,并引起广泛的生理变化。
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引用次数: 8
Sweet potato Weevil (Cylas puncticollis) Boheman Infestation: Cultivar Differences and the Effects of Mulching 甘薯象甲(Cylas puncticollis)波西米亚侵害:品种差异及覆盖效果
Pub Date : 2015-02-17 DOI: 10.2174/1874407901509010007
A. Mansaray, A. Sundufu, M. Moseray, S. Fomba
The effects of infestation by the sweet potato weevil, Cylas puncticollis Bohemen, on yield in 3 sweet potato cultivars, in relation to different mulching levels were compared. The experiment was laid out in a randomized complete split plot design at three levels. It consisted of a main plot factor, the three sweet potato cultivars (slipot 2, 3 and 4) with mulching at three levels (0, 1, 3 and 5t/ha) as sub plot. The factors were replicated thrice. The results of the studies re- vealed that increase in mulching level resulted in significantly decreased infestation of sweet potato weevil and higher root yield. Mulching at the rate of 5t/ha recorded the least infestation (12.13% for the year 2012; 13.32% for the year 2013) followed by 3t/ha (17.86% for the year 2012; 21.24% for the year 2013), 1t/ha (25.18% for the year 2012; 29.49% for the year 2013) whilst the unmulched plot recorded the highest (35.75% for the year 2012; 41.30% for the year 2013). Root yield was the highest with mulching at the rate of 5t/ha (6242.42Kg/ha for the year 2012; 4828.45Kg/ha for the year 2013) followed by 3t/ha (5863.64Kg/ha for the year 2012; 4883.82Kg/ha for the year 2013), 1t/ha (4580.81Kg/ha for the year 2012; 3818.18Kg/ha for the year 2013) whilst the unmulched plot recorded the highest (4202.03Kg/ha for the year 2012; 3489.89Kg/ha for the year 2013). Significant negative correlation (r = -0.91, p = 0.0001) was observed between weevil root damage and root dry matter. Only two cultivars, Slipot 3 and Slipot 4 were observed to be less susceptible to the sweetpoto weevil. Thus, using mulch cover at the rate of 3- 5t/ha in a field planted with slipot 3 or slipot 4 will in- crease yield, while at same time reduce sweet potato weevil infestation.
比较了不同覆盖水平下甘薯象甲对3个甘薯品种产量的影响。试验采用随机完全分形图设计,分为三个水平。以3个甘薯品种(2、3、4号)为主要小区因子,分别在0、1、3、5t/ hm2 3个水平覆盖,作为次小区。这些因素被重复了三次。结果表明,增加地膜覆盖水平可显著降低甘薯象鼻虫的侵染,提高根产量。覆盖量为5吨/公顷时,2012年虫害最少(12.13%);2013年为13.32%),其次是3吨/公顷(2012年为17.86%;2013年21.24%),1吨/公顷(2012年25.18%;2013年为29.49%),而未覆盖地块的比例最高(2012年为35.75%;2013年为41.30%)。2012年根产量最高,覆盖5t/ha (6242.42Kg/ha);2013年为4828.45公斤/公顷),其次是3吨/公顷(2012年为5863.64公斤/公顷;2013年为4883.82公斤/公顷),2012年为4580.81公斤/公顷;2013年为3818.18Kg/ha),而未覆盖地块记录最高(2012年为4202.03Kg/ha;2013年为3489.89公斤/公顷)。象鼻虫根系损伤与根系干物质呈显著负相关(r = -0.91, p = 0.0001)。只有2个品种,滑钵3号和滑钵4号被观察到对甜马铃薯象鼻虫不那么敏感。因此,在3号或4号滑槽种植的地块上,以3 ~ 5t/ hm2的覆盖量进行地膜覆盖,既能增产,又能减少甘薯象鼻虫的侵害。
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引用次数: 12
Factors Determining the Host Range of Two Tortoise Beetles, Cassidanebulosa L. and C. piperata Hope (Coleoptera: Chrysomelidae) in Japan 日本两种龟甲(鞘翅目:金龟甲科)寄主范围的影响因素
Pub Date : 2015-02-02 DOI: 10.2174/1874407901509010001
A. Nagasawa, K. Matsuda
We investigated the relationship between host-plant use by two tortoise beetles, Cassida nebulosa and C. piperata, in a field and their performance under laboratory conditions. Sixteen plant species were grown in the experimen- tal field with C. nebulosa being observed to grow on three chenopodiaceous species (Chenopodium album, C. album var. centrorubrum, and C. ficifolium), while C. piperata grew on the same three chenopodiaceous species plus three amaran- thaceous species (Amaranthus blitum, A. hybridus, and A. retroflexus). Host-plant selection in the field was directly corre- lated to the larval growth under laboratory conditions. Thus, the two tortoise beetles selected plant species that were suit- able as larval food under natural conditions. In the laboratory experiments, the survival period of adults and the number of eggs produced showed that adults have wider food ranges than larvae. Therefore, food quality is less important as a limit- ing factor for adults compared to larvae. In addition, the field observation showed that C. nebulosa laid eggs only on suit- able host plant species, whereas C. piperata laid eggs on both suitable hosts and non-hosts.
本文研究了两种陆龟(Cassida nebulosa和C. piperata)在野外对寄主植物的利用及其在实验室条件下的表现。在试验田中种植了16种植物,观察到云杉在3种藜属植物上生长(藜属植物、藜属植物var. centrorubrum和C. ficifolium),而花椒在3种藜属植物和3种苋属植物上生长(苋属植物blanthus、A. hybridus和A. retroflexus)。田间的寄主选择与实验室条件下的幼虫生长直接相关。因此,这两种陆龟甲虫选择了适合在自然条件下作为幼虫食物的植物物种。在室内实验中,成虫的生存期和产卵数表明,成虫的食物范围比幼虫更广。因此,与幼虫相比,食物质量作为成虫的限制因素不那么重要。此外,田间观察表明,云状假丝蛾只在适宜寄主植物上产卵,而胡椒假丝蛾在适宜寄主和非寄主植物上都产卵。
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引用次数: 2
Influence of Different Statuses of Honey Bee Queens, Apis mellifera L. on the Ultrastructure of the Flagella on (3-Day Old) Workers 不同蜂王状态对3日龄工蜂鞭毛超微结构的影响
Pub Date : 2014-11-14 DOI: 10.2174/1874407901408010022
Azza A. Awad, A. Moustafa, M. F. A. Rahman, R. Sayed
The effect of different statuses of honeybee queens, Apis mellifera (one-year-old mated queen; supersedure queen; three-day old virgin queen; queen cell; without queen; and without queen/without bee bread) on many aspects of 3- day old workers were studied with scanning electron microscopy (SEM). This study was carried out to describe the (types, distribution, numbers and measurements) of the different sensilla on the workers' flagellum. Electrone microscopic study of workers under different queen statuses showed the morphological structure of the antennae (Geniculate antennae). Each antenna composed of scape, a pedicel, and a flagellum of 10 segments. SEM revealed the presence of seven types and eleven subtypes of sensilla on the dorsal side of the different flagellomeres; they were Sensilla Ampullacea {Am}, Sen- silla Basiconica {Ba}, Sensilla Campaniformia {Cf}, Sensilla Chaetica I, II {Ch}, Sensilla Coelloconica I, II {Co}, Sen- silla Placodea I, II, III {PL}, Sensilla Trichodea I, II, III, IV {Tr}. This study revealed that, there is no significant differ- ence in the numbers of sensilla (Am), (Ba), (Co I) of the workers under all the queen statuses. The measurements of sen- silla Chaetica differ significantly according to the different queen statuses. The numbers and distributions of (Co II) were exhibited at extremely low percentages in the different statuses. There is significant difference in the percentages of each (PL I, II, III) between the different queen statuses. Also, the (Tr I, II, III, IV) are found in all statuses, There is significant difference in the percentages of each sensilla types between the different queen statuses.
蜂王不同状态的影响,1岁的蜜蜂(Apis mellifera;接替女王;三天老处女女王;女王细胞;没有皇后;用扫描电子显微镜(SEM)对3日龄工蜂的各方面进行了研究。本文对工蜂鞭毛上不同感受器的种类、分布、数量和尺寸进行了研究。对不同蜂后状态下工蜂的电子显微镜观察显示了触角的形态结构(膝状触角)。每根天线由花苞、花梗和10节鞭毛组成。扫描电镜显示在不同鞭毛节背侧存在7种类型和11种亚型的感受器;分别为壶形感器{Am}、Basiconica Sen- silla {Ba}、Campaniformia senilla {Cf}、Chaetica senilla I、II {Ch}、Coelloconica I、II {Co}、Placodea Sen- silla I、II、III {PL}、Trichodea senilla I、II、III、IV {Tr}。本研究发现,不同蜂后状态下工蜂的感受器(Am)、(Ba)、(Co I)数量无显著差异。根据不同的皇后地位,森新罗Chaetica的尺寸有很大的不同。不同状态下(Co II)的数量和分布都表现为极低的百分比。在不同的女王状态之间,每个(PL I, II, III)的百分比有显著差异。此外,在所有状态中都发现了(Tr I, II, III, IV),不同蜂后状态之间每种感受器类型的百分比存在显著差异。
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引用次数: 5
Googling Insects as a New Trend in Cultural Entomology: An Italian Perspective 谷歌昆虫作为文化昆虫学的新趋势:意大利视角
Pub Date : 2014-09-29 DOI: 10.2174/1874407901408010017
B. Luigi
Cultural entomology (CE) explores the interactions between the world of insects and mankind. Since the inter- net plays a major role in nowadays life, it would be of great interest to investigate the insects-related internet usage and activities. However, there are very few studies about this. For this reason, inspired by the seminal work carried out by the Japanese Takada, we decided to replicate his findings. We analyzed with Google Trends and with the wavelet power spec- trum analysis (WPSA) the hit-search volumes of the most common insects. We found that the five most commonly searched insects are bees, butterflies, mosquitoes, ants and spiders which are arachnids, but are commonly believed to be insects. We found a seasonality for some insects, as confirmed by the WPSA, and with peaks related to the seasonal pe- riod in which the insects are most visible, while we could not detect any regularity or trend for other insects, which are not clearly related to any particular season. In addition, we proved that, at least in some cases, hit-search volumes were influ- enced by the media and that the degree of seasonal regularity as measured by the WPSA was variable. Thus, we have proven the robustness of previous finding, extending their validity also in another culture and another context. Therefore, rather than being confined to a specific culture, googling insects could be a universal behavior and attitude, even though with some cross-cultural differences. Further systematic research is needed to shed light on this topic.
文化昆虫学(CE)探索昆虫世界与人类之间的相互作用。由于互联网在当今生活中扮演着重要的角色,因此研究与昆虫有关的互联网使用和活动将是非常有趣的。然而,这方面的研究很少。因此,受到日本高田的开创性工作的启发,我们决定复制他的发现。我们用谷歌趋势和小波功率谱分析(WPSA)分析了最常见昆虫的搜索量。我们发现最常搜索的五种昆虫是蜜蜂、蝴蝶、蚊子、蚂蚁和蜘蛛,它们是蛛形纲动物,但通常被认为是昆虫。我们发现了一些昆虫的季节性,正如WPSA所证实的那样,它们的峰值与昆虫最明显的季节类型有关,而我们没有发现任何规律性或趋势,它们与任何特定季节没有明显的关系。此外,我们证明,至少在某些情况下,点击搜索量受到媒体的影响,而WPSA测量的季节性规律性程度是可变的。因此,我们已经证明了先前发现的稳健性,并在另一种文化和另一种背景下扩展了它们的有效性。因此,尽管存在一些跨文化差异,但搜索昆虫可能是一种普遍的行为和态度,而不是局限于特定的文化。需要进一步的系统研究来阐明这一主题。
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引用次数: 2
Effects of Anti-Juvenile Hormone (Precocene II) Treatment on Phase Changes and Pheromone Production in the Desert Locust, Schistocerca gregaria (Forskal) (Orthoptera: Acrididae) 抗幼激素(早熟II)处理对荒漠蝗(直翅目:蝗科)生理期变化及信息素分泌的影响
Pub Date : 2014-07-11 DOI: 10.2174/1874407901408010010
A. I. Tawfik, E. Osir, A. Hassanali
The roles of anti-juvenile hormone (precocene II) on phase changes and pheromone production were examined in laboratory-bred gregarious desert locust, Schistocerca gregaria. Precocene II was applied to last instar nymphs and newly emerged adults in gregarious phase. Generally, the 5 th instar nymphs exhibited a higher sensitivity to topical appli- cation of precocen II than the adults. In contrast, the adults exhibited a higher sensitivity to injection of precocen II in comparison to 5 th instar nymphs. Topical application or injection of precocene II inhibited pheromone production (as measured by the amount of phenylacetonitrile released). Both topical application to 5 th instar nymphs and injection to adults affected the external body coloration, sexual behavior and mating of gregarious adult males, S. gregaria in com- parison to control insects.
研究了抗幼虫激素(早熟激素II)对荒漠蝗生理期变化和信息素产生的影响。在群居期末龄若虫和新出成虫中应用II型早熟。一般情况下,5龄若虫对外用II型早熟菌的敏感性高于成虫。与5龄若虫相比,成虫对注射II型早熟蛋白表现出更高的敏感性。外用或注射前茂素II抑制信息素的产生(通过苯乙腈的释放量来测量)。5龄若虫外用和成虫注射对群居成虫体表颜色、性行为和交配的影响与对照昆虫不同。
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引用次数: 1
Factors Affecting Abundance and Damage Caused by Cabbage Aphid, Brevicoryne brassicae on Four Brassica Leafy Vegetables: Brassica oleracea var. Acephala, B. chinense, B. napus and B. carinata 甘蓝蚜对4种芸苔类叶菜(甘蓝、白头甘蓝、中国甘蓝、甘蓝型甘蓝和油菜花甘蓝)丰度及危害的影响因素
Pub Date : 2014-06-13 DOI: 10.2174/1874407901408010001
D. Munthali, A. B. Tshegofatso
Resistance of four brassica leafy vegetables: kale, Chinese cabbage, rape and Ethiopian mustard to cabbage aphid, Brevicoryne brassicae (L.) was studied in cage experiments at the Botswana College of Agriculture, Gaborone, Botswana. A completely randomised design with cultivars as treatments and four replicates per treatment was used. One newly laid nymph was placed on each of the four potted plants of each cultivar and left to breed. Aphid abundance and damage on each cultivar were recorded weekly for six weeks. Direct counts of aphids were made to estimate abundance while the proportions of leaves with honeydew were used to measure intensity of damage. Chlorophyll, water and protein content and leaf thickness were estimated and their effects on aphid abundance and damage intensity were determined. Aphid populations on all cultivars peaked in the third week. Peak populations differed significantly (P < 0.05) between cultivars, with the greatest (828.2 per leaf) on Chinese cabbage and the least (307 per leaf) on Ethiopian mustard. Cabbage aphids caused significantly (P < 0.05) greater reduction of marketable leaves on kale than on the other cultivars. Cultivars with high chlorophyll; high water content and the thickest leaves had lower abundance of cabbage aphid than those with significantly (P< 0.05) lower chlorophyll and water content and thinner leaves; those with thicker leaves, high protein and high water content suffered greatest honeydew damage. Therefore breeding for low protein and water content, high chlo- rophyll content and thin leaves is recommended because the cultivar produced would suffer low honeydew damage.
在博茨瓦纳哈博罗内的博茨瓦纳农业学院,研究了甘蓝、白菜、油菜和埃塞俄比亚芥菜4种芸苔类叶菜对甘蓝蚜虫(Brevicoryne brassicae, L.)的抗性。试验采用完全随机设计,以品种为处理,每个处理4个重复。每个品种的四株盆栽植物上分别放置一个新产下的若虫,然后任其繁殖。连续6周每周记录各品种蚜虫数量及危害情况。蚜虫的直接计数被用来估计丰度,而带蜜露的叶子的比例被用来衡量损害的强度。测定叶绿素含量、水分和蛋白质含量以及叶片厚度,确定其对蚜虫数量和危害强度的影响。各品种蚜虫数量在第3周达到高峰。不同品种间最大种群数差异显著(P < 0.05),白菜最大(828.2只/叶),芥菜最小(307只/叶)。甘蓝蚜对甘蓝可售叶的影响显著(P < 0.05)高于其他品种。叶绿素含量高的品种;叶绿素和水分含量显著(P< 0.05)降低、叶片较薄的油菜蚜丰度显著低于叶片含水量较高、叶片较厚的油菜蚜丰度;叶片较厚、蛋白质含量高、水分含量高的植株受到的蜜露危害最大。因此,建议选育低蛋白质、低水分、高叶绿素含量、薄叶的品种,因为所生产的品种受蜜露危害小。
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引用次数: 14
First Report of the Fruits Flies, Ceratitis quinaria and Ceratitis Silvestri, on Yellow Plum Ximenia americana in Burkina Faso, West Africa 西非布吉纳法索美洲黄梅蝇属奎氏角膜炎和银角角膜炎首次报告
Pub Date : 2014-05-30 DOI: 10.2174/1874407901307010009
A. Sawadogo, O. Gnankiné, A. Badolo, A. Ouédraogo, S. Ouédraogo, R. Dabiré, A. Sanon
Fruits of Ximenia americana provide oil that is used for food and cosmetics. However, oil production potentials are limited by damages caused by the fruit flies. This research focused on the identification of the harmful insects of the ripe fruits of X. americana, the measure of morphometric and demographic parameters. Damages were also determined. Samplings were carried out in two sites and consisted in fruits collection on 10 randomly selected mature trees per site. Fruits are sorted to separate healthy ones and portions of damaged ones were incubated in order to follow insects rearing. Two species of fruit flies were identified for the first time on X. americana. They were Ceratitis quinaria (BEZZI) and Ceratitis silvestrii (BEZZI).Their morphometric and demographic parameters varied according to the sites. Parasitoids from the genus Diachasmimorpha were also identified. The rate of damaged fruits varied from 85.5% at Yagma to 95.5 % at Gampela, that compromises the efficient exploitation of this fruit. This work provides relevant information which will help to set up a sustainable management of X. americana's pests in Burkina Faso.
美洲西米尼亚的果实提供用于食品和化妆品的油。然而,由于果蝇造成的损害,石油生产潜力受到限制。本文主要对美洲凤梨成熟果实中有害昆虫的鉴定、形态计量学和人口统计学参数的测定进行了研究。损害赔偿金也已确定。采样在两个站点进行,包括在每个站点随机选择的10棵成熟树上采集果实。将水果分类,分离出健康的水果,并将部分受损的水果孵育,以便跟随昆虫饲养。在美洲蠓上首次鉴定出2种果蝇。分别为奎氏角膜炎(BEZZI)和西尔维斯特角膜炎(BEZZI)。它们的形态计量学和人口统计学参数因地点而异。同时还鉴定出了Diachasmimorpha属的拟寄生物。损坏水果的比率从Yagma的85.5%到Gampela的95.5%不等,这损害了这种水果的有效开发。这项工作提供了相关信息,将有助于在布基纳法索建立美洲大蠊害虫的可持续管理。
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引用次数: 3
Oxydoras kneri as a Potential Candidate for the Biological Control of Mosquito-Vector Tropical Diseases 克氏Oxydoras kneri作为蚊媒热带疾病生物防治的潜在候选者
Pub Date : 2013-09-06 DOI: 10.2174/1874407920130527001
A. M. Ribeiro, F. A. Leal-Santos, Paulo Roberto Soares, A. Martins, A. M. Nachi, Anagela Maria Acel, Jorge Senatore Vargas-Rodrigues, Eucilene Alves Santana Porto, Cor J. Fernandes Fontes
In this study, we assessed the larvivorous activity of Oxydoras kneri against Culicinae larvae in laboratory ex- periments to evaluate their suitability as a biological control. First, analysis of stomach content was performed to verify the feeding habits of the fish in their natural habitat. The behavior was then verified in aquarium experiments by feeding 2700 larvae to each fish. The feeding preference of the fish was evaluated in further experiments in which larvae and commercial fish food were offered, either separately or simultaneously. For both methods, the evaluation was performed by counting the number of adult mosquitoes that emerged during a 60-h observation period. The laboratory was main- tained at relatively stable temperature and humidity. The stomach content of 9 specimens of O. kneri captured in the Cuiaba River contained 26.5% insect larvae, with 11.5% exclusively from Diptera insects. The emergence of adults from insect larvae was not influenced by the presence of O. kneri. However, emergence was statistically lower in aquaria when the larvae were placed as food for the fish. The consumption of larvae and granulated commercial fish food was similar during a 60-h observation period, even when these items were offered separately or simultaneously. Since O. kneri are ubiquitous in the Amazon region, this finding highlights their potential role in the biological control of Culicidae mos- quito larvae growing in fish farm tanks. However, further studies are required to evaluate their behavior in the field and the operational difficulties of their use as a biological control.
本研究通过室内实验,评价了克氏氧毒蛾对库氏库氏幼虫的食虫活性,以评价其作为生物防治的适宜性。首先,对胃内容物进行分析,以验证鱼在其自然栖息地的摄食习性。然后在水族馆实验中通过向每条鱼喂2700条幼虫来验证这种行为。在进一步的实验中,分别或同时提供幼虫和商业鱼食,评估了鱼的摄食偏好。两种方法均采用60 h观察期成蚊出蚊数进行评价。实验室维持在相对稳定的温度和湿度下。在库亚巴河捕获的9只克纳利弧菌胃内容物中,昆虫幼虫含量为26.5%,其中双翅目昆虫占11.5%。昆虫幼虫成虫的羽化不受O. kneri菌存在的影响。然而,在水族箱中,当幼虫被放置为鱼的食物时,羽化率在统计上较低。在60小时的观察期间,幼虫和粒状商业鱼食的摄入量相似,即使这些食物是分开或同时提供的。由于O. kneri在亚马逊地区普遍存在,这一发现突出了它们在养鱼场水箱中生长的多基多库蚊幼虫的生物控制中的潜在作用。但是,还需要进一步的研究来评价它们在实地的行为和使用它们作为生物防治的操作困难。
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引用次数: 1
Larvicidal and Repellent Potential of Chenopodium ambrosioides Linn Essential Oil against Anopheles gambiae Giles (Diptera: Culicidae) 锦衣藜亚精油对冈比亚按蚊的杀幼虫驱避作用(双翅目:库蚊科)
Pub Date : 2013-08-23 DOI: 10.2174/1874407901307010016
J. Bigoga, Petola A. Saahkem, S. Ndindeng, J. Ngondi, M. Nyegue, J. Oben, R. Leke
Larvicidal and repellent effect of the essential oil from the seeds and leaves of Chenopodium ambrosioides Linn were evaluated against the larvae and adults of Anopheles gambiae s.s. mosquitoes at concentrations of 0, 50, 75, 100, 200, 300 and 400 ppm. Total mortality of larvae occurred at 200ppm and 300ppm for the essential oils from seeds and leaves respectively. Alpha-terpinene (73.55% for the seeds; 40.69% for the leaves) and para-cymene (20.05% for the seeds; 45.44% for the leaves) are the principal constituents. There was a 100% repellent effect on adult mosquitoes at 200ppm, with protection time of at least four hours for both seed and leaf essential oils. The findings indicated that C. ambrosioides Linn possess mosquito larvicidal and repellent potentials that might be further exploited in combating ma- laria through anti-vector intervention.
用0、50、75、100、200、300和400 ppm的浓度对冈比亚按蚊幼虫和成虫进行了杀幼虫和驱避效果的研究。种子精油含量为200ppm时幼虫总死亡率最高,叶片精油含量为300ppm时幼虫总死亡率最高。种子α -萜烯含量为73.55%;叶片40.69%)和副伞花素(种子20.05%);45.44%为叶片)是主要成分。当浓度为200ppm时,对成蚊的驱避效果为100%,种子精油和叶精油的保护时间至少为4小时。研究结果表明,锦绣草具有杀幼虫和驱避蚊虫的潜力,可进一步利用抗媒介干预手段防治疟疾。
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引用次数: 20
期刊
The Open Entomology Journal
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