Pub Date : 2019-06-30DOI: 10.26438/ijsrbs/v6i3.6572
S elvajeyanthi, N Hemashenpagam, M Vinotha
{"title":"Potential Probiotic Analysis Indigenous Lactic Acid Bacteria from Freshly Drawn Goat Milk","authors":"S elvajeyanthi, N Hemashenpagam, M Vinotha","doi":"10.26438/ijsrbs/v6i3.6572","DOIUrl":"https://doi.org/10.26438/ijsrbs/v6i3.6572","url":null,"abstract":"","PeriodicalId":14378,"journal":{"name":"International Journal of Scientific Research in Biological Sciences","volume":"2013 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73366593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-30DOI: 10.26438/ijsrbs/v6i3.2030
S. Ghouse
Received: 22/May/2019, Accepted: 12/Jun/2019, Online: 30/Jun/2019 Abstract-India is a country with more than 60–70% of its population dependent on agriculture. Rapid industrialization and successful green, white and blue revolutions have necessitated a large variety of chemicals/pesticides usage resulting environmental pollution which has become not only a national but a global problem. Fish occupy a prominent position in the field of aquatic toxicology and have been employed in studies concerning ecological health. In the present investigation two synthetic pyrethroids were selected namely Cyfluthrin and Fenvalerate to evaluate their toxicity on Gambusia affinis. In the present study there is significant decline in DNA content in the gill, liver and kidney tissues is observed, when the fish Gambusia affinis is exposed to the cyflthrin and fenvalerate. Decreases in the DNA content due to inhibition of the enzymes in DNA synthesis. The declines in RNA level in treated fish due to obstruction in RNA synthesis. Resulting in the swelling and chromatolysis of Nissle bodies which are rich in RNA. Pesticides may influence directly or modify DNA, other cellular process associated with the integrity of the genome.
{"title":"Effects of synthetic pyrethroids Cyfluthrin and Fenvalerate on Nucleic acid contents of fresh water fish Gambusia affinis","authors":"S. Ghouse","doi":"10.26438/ijsrbs/v6i3.2030","DOIUrl":"https://doi.org/10.26438/ijsrbs/v6i3.2030","url":null,"abstract":"Received: 22/May/2019, Accepted: 12/Jun/2019, Online: 30/Jun/2019 Abstract-India is a country with more than 60–70% of its population dependent on agriculture. Rapid industrialization and successful green, white and blue revolutions have necessitated a large variety of chemicals/pesticides usage resulting environmental pollution which has become not only a national but a global problem. Fish occupy a prominent position in the field of aquatic toxicology and have been employed in studies concerning ecological health. In the present investigation two synthetic pyrethroids were selected namely Cyfluthrin and Fenvalerate to evaluate their toxicity on Gambusia affinis. In the present study there is significant decline in DNA content in the gill, liver and kidney tissues is observed, when the fish Gambusia affinis is exposed to the cyflthrin and fenvalerate. Decreases in the DNA content due to inhibition of the enzymes in DNA synthesis. The declines in RNA level in treated fish due to obstruction in RNA synthesis. Resulting in the swelling and chromatolysis of Nissle bodies which are rich in RNA. Pesticides may influence directly or modify DNA, other cellular process associated with the integrity of the genome.","PeriodicalId":14378,"journal":{"name":"International Journal of Scientific Research in Biological Sciences","volume":"53 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89582299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-30DOI: 10.26438/ijsrbs/v6i3.114118
Swarnalatha Daram, S. Poornima, Syeda Zubeda, P Akhilesh, Ayyala, B. Srinadh, P. Shailaja, A. Sandhya, D. Ramakrishna, Q. Hasan
{"title":"Association of Regulatory Factor X 6 gene (rs339331) polymorphism with Prostate Cancer A case-control study from South India","authors":"Swarnalatha Daram, S. Poornima, Syeda Zubeda, P Akhilesh, Ayyala, B. Srinadh, P. Shailaja, A. Sandhya, D. Ramakrishna, Q. Hasan","doi":"10.26438/ijsrbs/v6i3.114118","DOIUrl":"https://doi.org/10.26438/ijsrbs/v6i3.114118","url":null,"abstract":"","PeriodicalId":14378,"journal":{"name":"International Journal of Scientific Research in Biological Sciences","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73064805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-30DOI: 10.26438/ijsrbs/v6i3.119123
S. Puvaneswari, R. Karuppasamy
{"title":"Length-Weight Relationship of the Toothed Pony Fish, Gazza Minuta (Bloch, 1797) from Tuticorin Waters of India","authors":"S. Puvaneswari, R. Karuppasamy","doi":"10.26438/ijsrbs/v6i3.119123","DOIUrl":"https://doi.org/10.26438/ijsrbs/v6i3.119123","url":null,"abstract":"","PeriodicalId":14378,"journal":{"name":"International Journal of Scientific Research in Biological Sciences","volume":"258 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79589830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-30DOI: 10.26438/ijsrbs/v6i3.8186
Ashok Dawande, Neha D. Gajbhiye, V. N. Charde, Y. S. Banginwar
The present study reports assessment of the antibiofilm activity of endophytic fungi on quorum sensing controlled biofilm formation in Pseudomonas aeruginosa MTCC 2453. Endophytic fungi isolated from agriculture field were evaluated for antibiofilm activity. After fermentation in sucrose broth, biofilm formation inhibitors from endophytic fungi were extracted using ethyl acetate. The active extract from each endophytic fungus was assayed for inhibition of biofilm formation using biosensor strain Pseudomonas aeruginosa. At highest tested concentration (200μg/ml) of both fungal extract and biocidal agent, the extract from Fusarium sp.1 demonstrated maximum inhibition in biofilm formation (49.65%) and minimum inhibition by Colletotrichum sp. extract (18.21%) and inhibition was significant at (P < 0.05). Similarly at highest tested concentration (200μg/ml) of fungal extract alone, the maximum percent decrease (18.90%) was observed in P. aeruginosa by Phoma sp. extract and minimum inhibition by Penicillium sp extract (4.00%) with no statistical difference. On the other hand extract from Aspergillus sp.2 was found to show no antibiofilm activity but exhibit stimulatory effect on biofilm formation extract (-4.78%). Combined effect of active fungal extract and biocidal agent resulted in significantly more percent biofilm inhibition as compared to fungal extract alone. The action of one or more active compounds extracted from endophytic fungi may be responsible for antibiofilm activity. Such compounds could be effective against emerging multidrug resistant pathogens. KeywordsEndophytic fungi, Biofilm, Quorum sensing, Pseudomonas aeruginosa.
{"title":"Assessment of endophytic fungal isolates for its Antibiofilm activity on Pseudomonas aeruginosa","authors":"Ashok Dawande, Neha D. Gajbhiye, V. N. Charde, Y. S. Banginwar","doi":"10.26438/ijsrbs/v6i3.8186","DOIUrl":"https://doi.org/10.26438/ijsrbs/v6i3.8186","url":null,"abstract":"The present study reports assessment of the antibiofilm activity of endophytic fungi on quorum sensing controlled biofilm formation in Pseudomonas aeruginosa MTCC 2453. Endophytic fungi isolated from agriculture field were evaluated for antibiofilm activity. After fermentation in sucrose broth, biofilm formation inhibitors from endophytic fungi were extracted using ethyl acetate. The active extract from each endophytic fungus was assayed for inhibition of biofilm formation using biosensor strain Pseudomonas aeruginosa. At highest tested concentration (200μg/ml) of both fungal extract and biocidal agent, the extract from Fusarium sp.1 demonstrated maximum inhibition in biofilm formation (49.65%) and minimum inhibition by Colletotrichum sp. extract (18.21%) and inhibition was significant at (P < 0.05). Similarly at highest tested concentration (200μg/ml) of fungal extract alone, the maximum percent decrease (18.90%) was observed in P. aeruginosa by Phoma sp. extract and minimum inhibition by Penicillium sp extract (4.00%) with no statistical difference. On the other hand extract from Aspergillus sp.2 was found to show no antibiofilm activity but exhibit stimulatory effect on biofilm formation extract (-4.78%). Combined effect of active fungal extract and biocidal agent resulted in significantly more percent biofilm inhibition as compared to fungal extract alone. The action of one or more active compounds extracted from endophytic fungi may be responsible for antibiofilm activity. Such compounds could be effective against emerging multidrug resistant pathogens. KeywordsEndophytic fungi, Biofilm, Quorum sensing, Pseudomonas aeruginosa.","PeriodicalId":14378,"journal":{"name":"International Journal of Scientific Research in Biological Sciences","volume":"111 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73955667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-30DOI: 10.26438/ijsrbs/v6i3.130132
Kalpana I. Pattan, S. Puranik
In this study, we surveyed 47 women beedi workers of Nippani, a place in North Karnataka of India known for tobacco trade and industries. We found that this labor intensive task has led to enormous health conditions in the women involved in rolling the beedis manually. Respiratory problems like cough and throat burns were reported by more than 50% of women. The incidence of Orthopedic and Ophthalmic illness was also common. Other health conditions included GI problems, Giddiness and Headache. Keywords— Beedi-workers, Tobacco, Respiratory problems, orthopedic problems, Indian small scale industry.
{"title":"Occupational Health Hazards of Women Beedi Rollers in Nippani Taluk, South India","authors":"Kalpana I. Pattan, S. Puranik","doi":"10.26438/ijsrbs/v6i3.130132","DOIUrl":"https://doi.org/10.26438/ijsrbs/v6i3.130132","url":null,"abstract":"In this study, we surveyed 47 women beedi workers of Nippani, a place in North Karnataka of India known for tobacco trade and industries. We found that this labor intensive task has led to enormous health conditions in the women involved in rolling the beedis manually. Respiratory problems like cough and throat burns were reported by more than 50% of women. The incidence of Orthopedic and Ophthalmic illness was also common. Other health conditions included GI problems, Giddiness and Headache. Keywords— Beedi-workers, Tobacco, Respiratory problems, orthopedic problems, Indian small scale industry.","PeriodicalId":14378,"journal":{"name":"International Journal of Scientific Research in Biological Sciences","volume":"99 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80576707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-30DOI: 10.26438/ijsrbs/v6i3.5664
Amardeep Singh, Gyanika Shukla, S. S. Gaurav
{"title":"To Raise the Polyclonal Immuno-Probe(s) and Development of Enzyme Immuno-Assay for Detection of Cross Reactivity of Alternaria brassicae and their Different Isolates","authors":"Amardeep Singh, Gyanika Shukla, S. S. Gaurav","doi":"10.26438/ijsrbs/v6i3.5664","DOIUrl":"https://doi.org/10.26438/ijsrbs/v6i3.5664","url":null,"abstract":"","PeriodicalId":14378,"journal":{"name":"International Journal of Scientific Research in Biological Sciences","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86113313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-30DOI: 10.26438/ijsrbs/v6i3.4149
V. Devaraj, S. S. Kesti
As termite is a social insect, its colony consists of three castes. i.e. reproductive, worker & soldier castes. In their role of cellulose digestion, the worker termite uses two sources of cellulolytic enzyme that include cellulases produced by the termite and the gut symbionts. Odontotermes obesus classified in mound builder termite, mostly depend on cellulolytic bacteria for cellulose digestion. This study aims to characterize cellulolytic bacteria of termite gut symbionts of worker Odontotermes obesus and to identify cellulolytic bacteria based on sequences of 16S ribosomal RNA(rRNA) gene. Cellulolytic bacteria of termite gut were isolated and cultured in CMC media. Gram’s staining and biochemical characteristics were analysed. The two bacterial isolates were selected from Odontotermes obesus with aerobic conditions for the analysis of 16S rRNA gene sequences. BLAST-N result of 16S rRNA gene sequences of white and yellow colony isolates showed that the isolate has highest similarity with Trabulsiella odontotermitis and Chryseobacterium ureilyticum, respectively.
{"title":"Isolation and Molecular Characterization of Termite GUT Microflora","authors":"V. Devaraj, S. S. Kesti","doi":"10.26438/ijsrbs/v6i3.4149","DOIUrl":"https://doi.org/10.26438/ijsrbs/v6i3.4149","url":null,"abstract":"As termite is a social insect, its colony consists of three castes. i.e. reproductive, worker & soldier castes. In their role of cellulose digestion, the worker termite uses two sources of cellulolytic enzyme that include cellulases produced by the termite and the gut symbionts. Odontotermes obesus classified in mound builder termite, mostly depend on cellulolytic bacteria for cellulose digestion. This study aims to characterize cellulolytic bacteria of termite gut symbionts of worker Odontotermes obesus and to identify cellulolytic bacteria based on sequences of 16S ribosomal RNA(rRNA) gene. Cellulolytic bacteria of termite gut were isolated and cultured in CMC media. Gram’s staining and biochemical characteristics were analysed. The two bacterial isolates were selected from Odontotermes obesus with aerobic conditions for the analysis of 16S rRNA gene sequences. BLAST-N result of 16S rRNA gene sequences of white and yellow colony isolates showed that the isolate has highest similarity with Trabulsiella odontotermitis and Chryseobacterium ureilyticum, respectively.","PeriodicalId":14378,"journal":{"name":"International Journal of Scientific Research in Biological Sciences","volume":"78 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83897441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-30DOI: 10.26438/ijsrbs/v6i3.3140
M. Ramaswamy, Sivakumar Duraikannu, Chandrasekar Solaimuthu
Indian saw-scaled viper are one of the highly venomous snake found in Indian subcontinent and its venom has a wide variety of toxic compounds and it can able to causing severe envenoming characterized by severe pharmacological effects. The traditional knowledge on the Acalypha fruticosa plant was clearly states that this plant was used against the venomous bites and stings. In best of our knowledge, there were no any scientific reports to justify the anti-snake venom potential of this plant. The main objective of the present research work was to evaluate the anti-snake venom activity of A. fruticosa against Indian saw-scaled viper venom using envenomed animal model. The effects of ethanolic leaves extract of A. fruticosa on hematological parameters of experimental rats, saw-scaled viper venom induced liver toxicity, renal toxicity and blood metabolites was analyzed in experimental rats. In the present study, the ethanolic leaves extract of A. fruticosa leaves was inhibited the venom induced changes in hematological parameters. The plant extract was effectively decreased the saw-scaled viper venom induced liver toxicity and renal toxicity in experimental rats. The observed results clearly states that the A. fruticosa leaves has potent snati-snake venom activity.
{"title":"Assessment of anti-snake venom effects of Acalypha fruticosa leaves against Indian Saw-scaled viper by using envenomed Wistar albino rats","authors":"M. Ramaswamy, Sivakumar Duraikannu, Chandrasekar Solaimuthu","doi":"10.26438/ijsrbs/v6i3.3140","DOIUrl":"https://doi.org/10.26438/ijsrbs/v6i3.3140","url":null,"abstract":"Indian saw-scaled viper are one of the highly venomous snake found in Indian subcontinent and its venom has a wide variety of toxic compounds and it can able to causing severe envenoming characterized by severe pharmacological effects. The traditional knowledge on the Acalypha fruticosa plant was clearly states that this plant was used against the venomous bites and stings. In best of our knowledge, there were no any scientific reports to justify the anti-snake venom potential of this plant. The main objective of the present research work was to evaluate the anti-snake venom activity of A. fruticosa against Indian saw-scaled viper venom using envenomed animal model. The effects of ethanolic leaves extract of A. fruticosa on hematological parameters of experimental rats, saw-scaled viper venom induced liver toxicity, renal toxicity and blood metabolites was analyzed in experimental rats. In the present study, the ethanolic leaves extract of A. fruticosa leaves was inhibited the venom induced changes in hematological parameters. The plant extract was effectively decreased the saw-scaled viper venom induced liver toxicity and renal toxicity in experimental rats. The observed results clearly states that the A. fruticosa leaves has potent snati-snake venom activity.","PeriodicalId":14378,"journal":{"name":"International Journal of Scientific Research in Biological Sciences","volume":"128 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85283027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-30DOI: 10.26438/ijsrbs/v6i3.113
L. Das, H. Salvi, R. D. Kamboj
The study described the diversity and phytosociological attributes of plant species (trees, shrubs and herbs) in coastal areas of Devbhoomi Dwarka District and its islands in the Gulf of Kachchh. A random sampling method was employed in this study. A total of 243 plant species were recorded of which trees and shrubs represented with 30 specieseach. Grasses & sedges were also represented by 30 species and 29 species were climbers. Among the tree and shrub species, Prosopis juliflora showed the highest density (373.51 ind. /ha), frequency (63.50.67%), relative density (30.19.7%), relative frequency (24.41%) and relative abundance (7.68%).Regarding herb species, Aristida redacta represented the highest density (3.97ind./sq.m) and frequency (39.02%). Moreover, the highest importance value index was measured in Prosopis juliflora (62.28) among trees & shrubs and Aristida redacta (31.51) among herbs. The Abundance/Frequency ratio of trees, shrubs and herb species showed contagious distribution pattern within the study area. The present study also includes α diversity (Shannon diversity index, Simpson's Index, species richness, evenness index) of the coastal terrestrial plants.
{"title":"Phytosociological study of coastal flora of Devbhoomi Dwarka district and its islands in the Gulf of Kachchh, Gujarat","authors":"L. Das, H. Salvi, R. D. Kamboj","doi":"10.26438/ijsrbs/v6i3.113","DOIUrl":"https://doi.org/10.26438/ijsrbs/v6i3.113","url":null,"abstract":"The study described the diversity and phytosociological attributes of plant species (trees, shrubs and herbs) in coastal areas of Devbhoomi Dwarka District and its islands in the Gulf of Kachchh. A random sampling method was employed in this study. A total of 243 plant species were recorded of which trees and shrubs represented with 30 specieseach. Grasses & sedges were also represented by 30 species and 29 species were climbers. Among the tree and shrub species, Prosopis juliflora showed the highest density (373.51 ind. /ha), frequency (63.50.67%), relative density (30.19.7%), relative frequency (24.41%) and relative abundance (7.68%).Regarding herb species, Aristida redacta represented the highest density (3.97ind./sq.m) and frequency (39.02%). Moreover, the highest importance value index was measured in Prosopis juliflora (62.28) among trees & shrubs and Aristida redacta (31.51) among herbs. The Abundance/Frequency ratio of trees, shrubs and herb species showed contagious distribution pattern within the study area. The present study also includes α diversity (Shannon diversity index, Simpson's Index, species richness, evenness index) of the coastal terrestrial plants.","PeriodicalId":14378,"journal":{"name":"International Journal of Scientific Research in Biological Sciences","volume":"2014 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73313944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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