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Antibacterial Activity of Fungal Endophytes Isolated from Wattakaka volubilis (Linn.f.), A medicinal plant from Telangana, India. 来自印度特伦加纳邦的药用植物卷叶草(Linn.f.)内生真菌的抗菌活性研究。
Pub Date : 2019-02-28 DOI: 10.26438/ijsrbs/v6i1.7583
A. Aruna, M. Abhinesh, V. Bhavani, V. K. Reddy
Received: 23/Jan/2019, Accepted: 10/Feb/2019, Online: 28/Feb/2019 Abstract─ Endophytic fungi are known to produce a wide range of metabolites of pharmacological importance. Endophytes associated with medicinal plants are targets for intensive research. This research paper deals with the endophytic fungi isolated from Wattakaka volubili also known as Dudipala, a high valued medicinal plant in traditional medicine. A total of 39 fungal species representing mostly imperfect fungi were isolated from different plant parts. The fungal species varied with geographic and environmental conditions of the same host species. Among different genera, Aspergillus, Penicillium, Fusarium, Phoma and mycelia sterilia were dominant. Solvent extracts of five endophytic fungi viz Aspergillus oryzae, Diplodia andamanensis, Fusarium graminearum, Penicillium rubrum and Rhizoctonia bataticola were tested against four human pathogenic bacteria, Antibacterial activity varied with the fungus as well as solvent. The inhibitory activity is very prominent on E.coli. Hexanol and chloroform extracts activity is very close to streptomycin. In light of these observations it is recommended that the pure extracts need to further test for antibacterial activity.
收稿日期:2019年1月23日,收稿日期:2019年2月10日,在线日期:2019年2月28日摘要─内生真菌可以产生多种具有药理意义的代谢物。与药用植物相关的内生菌是深入研究的目标。本研究研究了一种传统药用植物——水仙(Wattakaka volubili)的内生真菌。共分离到39种真菌,其中大部分为不完全真菌。同一寄主的地理和环境条件不同,真菌种类也不同。在不同属中,曲霉属、青霉属、镰刀菌属、真菌属和无菌菌丝属占优势。对5种内生真菌(米曲霉、红豆双plodia andamanensis、禾谷镰刀菌、红青霉和bataticola Rhizoctonia)的溶剂提取物进行了对4种人类致病菌的抑菌活性试验,结果表明,不同溶剂和真菌的抑菌活性不同。对大肠杆菌的抑制作用非常明显。己醇和氯仿提取物的活性与链霉素非常接近。根据这些观察结果,建议需要进一步测试纯提取物的抗菌活性。
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引用次数: 1
Association of AVPR1A gene microsatellites with Autism Spectrum Disorder in south Indian population 南印度人群中AVPR1A基因微卫星与自闭症谱系障碍的关系
Pub Date : 2019-02-28 DOI: 10.26438/ijsrbs/v6i1.203207
.. D.Mandamkulathil, .. AA.Pillai, .. SA.Poovathinal, .. SK.Chengappa, .. A.Chandrasekar, Asn Murthy, Dzampaeva Et
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引用次数: 0
In Vitro Conservation of Medicinally Important Climbing Shrub Maerua Arenaria Hook. F. and Thomson 药用灌木沙棘的离体保护。F.和汤姆森
Pub Date : 2019-02-28 DOI: 10.26438/ijsrbs/v6i1.208212
K. Thirupathi, M. Ghani, V. Suresh, T. Swamy, G. Rajender, Muhammad Mustafa
Caper family belongs to the order Brassicales comprises various important medicinal plants distributed in tropical countries. Maerua is the second largest genera in this family. Maerua arenaria is a wild climbing shrub having high medicinal value, due to over exploitation it becomes threatened. In vitro propagation technique has been adopted for conserving the valuable medicinal plant by using plant growth regulators. Initially the nodal explants were inoculated on MS medium supplemented with 4.0 mg/l BAP and 2.0 mg/l NAA for axillary bud proliferation. Multiple shoots (5-6 shoots per explant) were recorded after 34 days on MS medium with 2.0 mg/l BAP and 2.0 mg/l IAA. Since the shoots are small, they were transferred on MS medium containing 2.0 mg/l BAP and 2.0 mg/l GA3 for shoot elongation. These well developed elongated shoots were transferred to MS medium supplemented with 2.0 mg/l IBA for rooting. These in vitro developed plantlets were acclimatized in green house and successfully transplanted to natural condition and 78% of plants were survived.
刺山柑科属于芸苔目,是分布在热带国家的多种重要药用植物。Maerua是这个科的第二大属。毛蕊花是一种具有很高药用价值的野生攀缘灌木,由于过度开发而受到威胁。利用植物生长调节剂对珍贵药用植物进行体外繁殖保护。先将外植体接种于添加4.0 mg/l BAP和2.0 mg/l NAA的MS培养基上,培养腋芽增殖。在添加2.0 mg/l BAP和2.0 mg/l IAA的MS培养基上,培养34天后可形成多苗(每个外植体5-6个苗)。由于芽小,将其转移到含有2.0 mg/l BAP和2.0 mg/l GA3的MS培养基上进行芽伸长。将发育良好的细长芽转移到添加2.0 mg/l IBA的MS培养基上生根。这些离体发育植株经温室驯化后成功移栽到自然条件下,成活率达78%。
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引用次数: 2
Diversity of Lichens along Elevational Gradients in Forest Ranges of Chamarajanagar District, Karnataka State 卡纳塔克邦查玛拉贾纳加尔地区森林山脉沿海拔梯度的地衣多样性
Pub Date : 2019-02-28 DOI: 10.26438/ijsrbs/v6i1.97104
S. Rashmi, H. Rajkumar
The lichen species richness in Chamarajanagar district in Karnataka, India were assessed using altitudinal gradient, in order to compare distribution patterns of different growth forms, dominant families and diversity index. Four major forest ranges, Biligiriranga Hills, Himavad Gopalaswamy Hills, Malay Mahadeshwara Hills, and Shivanasamudra Falls were surveyed and a total of 97 lichens, belonging to 47 genera and 25 families were recorded. Physciaceae was found to be dominant family by 18 species under 8 genera, followed by Parmeliaceae with 16 species under 4 genera. Crustose type was dominated with 51%, followed by foliose type with 38%. Malay Mahadeshwara hills had highest lichen species richness and harboured 67 species, of which, 36 species were crustose type and 28 species were foliose type. Shannon-weiner index ranged from 3.852.45 and Simpson index of diversity ranged from 0.02-0.09. Luxuriant growth of lichens was recorded at the altitude of mid elevation with degree of abundance, density, frequency and distribution of the lichen species compared to other elevation. The present study provided baseline data of lichen diversity, which helps in understanding the relationship between distribution of lichen species along different elevation bands and vegetation types. Keywords— Biodiversity, Deciduous forest, Physciaceae, Corticolous, MM Hills
采用海拔梯度法对印度卡纳塔克邦Chamarajanagar地区地衣物种丰富度进行了评价,以比较不同生长形式、优势科和多样性指数的分布格局。调查了Biligiriranga Hills、Himavad Gopalaswamy Hills、马来Mahadeshwara Hills和Shivanasamudra Falls 4个主要森林山脉,共记录了97种地衣,隶属于25科47属。结果表明,薄荷科为优势科,8属18种,4属16种;壳型占51%,其次是卵叶型,占38%。马来Mahadeshwara山地衣物种丰富度最高,共有67种地衣,其中壳状地衣36种,叶状地衣28种。Shannon-weiner多样性指数为3.852.45,Simpson多样性指数为0.02 ~ 0.09。中高海拔地衣生长繁茂,种类丰富度、密度、频次和分布均高于其他海拔。本研究提供了地衣多样性的基线数据,有助于了解不同海拔带地衣物种分布与植被类型之间的关系。关键词:生物多样性,落叶林,Physciaceae,皮质,MM Hills
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引用次数: 5
Conspectus of Aquatic Macrophytic Flora of Jammu and Kashmir State, India 印度查谟和克什米尔邦水生大型植物区系概貌
Pub Date : 2019-02-28 DOI: 10.26438/ijsrbs/v6i1.167176
A. Shah, Z. Reshi, M. Shah
Keeping in view importance of macrophytic in aquatic ecosystems, we focused on the diversity of macrophytes in Jammu and Kashmir State, the part western Himalaya. A total of 234 macrophytic species were reported from all three regions of state after taken into consideration both primary and secondary sources. The emergent macrophytes had the highest diversity, followed by rooted floating leaftype, submerged and free floating. The maximum numbers of macrophytic species were recorded in the Kashmir valley while as minimum of 41 species from the Ladakh region. The lakes which have been submitted to an increasing eutrophication for the past several years is overgrown mainly by the aggressive interlopers like Typha sp., Phragmites australis, Sparganium ramosum and Scirpus sp and some free floating species.
考虑到水生生态系统中大型植物的重要性,我们将重点放在喜马拉雅西部查谟和克什米尔邦大型植物的多样性上。在考虑了原生和次生来源后,全州三个地区共报告了234种大型植物。苗生植物多样性最高,其次为根浮叶型、沉叶型和自由漂浮型。大植植物种类最多的是克什米尔谷地,最少的是拉达克地区,只有41种。近年来,湖泊富营养化程度呈上升趋势,主要为泰蒲、芦苇、沙米草和山棘等入侵物种和一些自由漂浮物种。
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引用次数: 3
Alteration in Antioxidant Defence System and Oxidative Stress in blood of Liver Cirrhosis 肝硬化患者血液中抗氧化防御系统及氧化应激的改变
Pub Date : 2019-02-28 DOI: 10.26438/ijsrbs/v6i1.4754
A. Thakkar, H. Shah, N. Patel
Available online at: www.isroset.org Received: 10/Jan/2019, Accepted: 11/Feb/2019, Online: 28/Feb/2019 AbstractSerum liver marker enzymes ALT, AST, ALP and GGT are generally used as liver marker enzymes however they cannot provide exact idea regarding establishment of liver disease condition because many liver cirrhotic participants indicate non-significant alteration. Present study is aimed to know the level of antioxidant defense system and oxidative stress in controls and cirrhotic participants. The cross-sectional study was carried out at tertiary centre of gastroenterologist unit at Anand. Experimental participants were divided into two groups: Control (n= 52, Age26.7±1.23 years, 39 M/ 13 F) and cirrhosis (n= 44, Age53.48±2.02 years, 34 M/10 F). All participants underwent anthropometric assessment, clinical examination, biochemical parameters, antioxidant parameters and lipid peroxidation marker or oxidative stress marker. Results of our study indicates nonconclusive increase in serum enzyme level. As an average only 42-67 % of the participants indicates increase (ALT (55%), AST (67.5%), GGT (57.5%) and ALP (42.5%)). The total antioxidant activity (Gallic acid equivalent (GAE (p<0.0001)) and Trolox equivalent (TE) (p<0.0001)) and antioxidant enzymes such as Catalase (CAT (p<0.0001)), Glutathione Peroxidase (GSHPx (p<0.0001)) and Glutathione-s-transferase (GST (p<0.0001)) were significantly decreased except superoxide dismutase (SOD (p<0.05)) during cirrhosis condition. Lipid peroxidation marker or oxidative stress marker (MDA) was significantly increased (p<0.0001) in cirrhotic participants compared to controls. The alteration in the above parameters is among all (100%) the participants. Study strongly indicates that antioxidant enzymes namely GST, GSHPx and CAT can be used as surrogate markers of oxidative stress for the cirrhosis condition.
摘要血清肝脏标记酶ALT、AST、ALP和GGT通常被用作肝脏标记酶,但由于许多肝硬化参与者表示没有明显改变,因此它们不能提供肝脏疾病状况的确切信息。本研究旨在了解对照组和肝硬化参与者的抗氧化防御系统和氧化应激水平。横断面研究是在阿南德胃肠病学三级中心进行的。实验参与者分为对照组(n= 52,年龄26.7±1.23岁,39 M/ 13 F)和肝硬化组(n= 44,年龄53.48±2.02岁,34 M/10 F)。所有参与者均进行了人体测量、临床检查、生化指标、抗氧化指标和脂质过氧化指标或氧化应激指标。我们的研究结果表明血清酶水平的增加不是决定性的。平均只有42- 67%的参与者表示ALT(55%)、AST(67.5%)、GGT(57.5%)和ALP(42.5%)升高。总抗氧化活性(没食子酸等效(GAE (p<0.0001))和Trolox等效(TE) (p<0.0001))以及过氧化氢酶(CAT (p<0.0001))、谷胱甘肽过氧化物酶(GSHPx (p<0.0001))和谷胱甘肽s-转移酶(GST (p<0.0001))等抗氧化酶(除超氧化物歧化酶(SOD (p<0.05))外)均显著降低。与对照组相比,肝硬化参与者的脂质过氧化标志物或氧化应激标志物(MDA)显著升高(p<0.0001)。以上参数的改变是所有(100%)参与者的。研究强烈提示抗氧化酶GST、GSHPx和CAT可以作为肝硬化氧化应激的替代指标。
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引用次数: 0
Anatomy and stomatal micromorphology of Psophocarpus tetragonolobus (L.) DC. (Winged Bean) 四爪蟾的解剖与气孔微观形态直流。(带翅膀的Bean)
Pub Date : 2019-02-28 DOI: 10.26438/ijsrbs/v6i1.233239
K. Kumar, R. ajalakshmi
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引用次数: 1
Characterization and fungistatic activity of Eucalyptus globulus leaf extract mediated phytosynthesized silver nanoparticles 桉叶提取物介导的植物合成纳米银的表征及抑菌活性研究
Pub Date : 2019-02-28 DOI: 10.26438/ijsrbs/v6i1.105111
M. Kumar, R. Garampalli
In the present study silver nanoparticles were synthesized through green approach using leaf extract of Eucalyptus globules and confirmed by change in colour of reaction mixture, the peak obtained at 425nm in UV-visible spectroscopic study and characterized by XRD, DLS, SEM, EDAX and FTIR for their structure, size, shape and possible biomolecules responsible for the reduction of silver ion to silver nanoparticles. XRD spectrum confirmed the particles were face centered cubic in nature with an average particle size of 25nm which is in agreement with DLS data (25.31nm). SEM images of nanoparticles revealed the spherical shape of particles and the Energy Dispersive X-ray analysis (EDAX) confirmed the significant presence of elemental silver. Synthesized silver nanoparticle showed good fungicidal activity against Didymella bryoniae, Fusarium oxysporum, Aspergillus flavus and Rhizoctonia solani. KeywordsFungistatic, activity, Phytosynthesis, Silver nanoparticles, Eucalyptus globulus
本研究以桉树叶提取物为原料,采用绿色法合成了银纳米颗粒,并通过反应混合物颜色的变化进行了证实,在425nm处获得了紫外可见光谱峰,并通过XRD、DLS、SEM、EDAX和FTIR对其结构、大小、形状和可能参与银离子还原成银纳米颗粒的生物分子进行了表征。XRD谱图证实颗粒为面心立方,平均粒径为25nm,与DLS数据(25.31nm)一致。纳米颗粒的SEM图像显示颗粒呈球形,能量色散x射线分析(EDAX)证实了元素银的显著存在。合成的纳米银颗粒对褐裂菌、尖孢镰刀菌、黄曲霉和枯丝核菌均有良好的杀菌活性。关键词抑菌,活性,植物合成,纳米银,蓝桉
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引用次数: 0
Synthesis and characterization of Cadmium (II) complex with tetradentate N2O2- donor Schiff base and biological activity 四齿N2O2-施主席夫碱镉配合物的合成、表征及生物活性
Pub Date : 2019-02-28 DOI: 10.26438/ijsrbs/v6i1.270274
Animesh Patra, G. P. Sahoo, Palash Setua, A. Pandey, Surajit Maiti
Transition metal complex of Cd (II) with tetradentate schiff base derived from the condensation of 4-nitro-1,2diamino benzene and 5-nitro-salicyldehyde (H2L) have been synthesized. The synthesized square planar complex was investigated using elemental analysis (C, H and N), infrared, conductivity and electronic spectroscopic tools. The interactions of cadmium (II) complex examined towards Bovine serum albumin (BSA) with the help of absorption and fluorescence spectroscopic tools. The complex is strongly binds to proteins, then we study the antibacterial activity of the Schiff base and its cadmium complex by agar disc diffusion method against some species of pathogenic bacteria (Escherichia coli, Vibrio cholerae, Streptococcus pneumonia and Bacillus cereus).The result indicated that cadmium complex was more activity than ligand due to higher lipophilicity effect. Keywords— Cadmium complex, Bovine serum albumin interaction, antibacterial activity.
由4-硝基-1,2 -二氨基苯和5-硝基水杨醛(H2L)缩合而成的四齿席夫碱过渡金属Cd (II)配合物。采用元素分析(C、H、N)、红外、电导率和电子能谱等手段对合成的方平面配合物进行了表征。利用吸收光谱和荧光光谱研究了镉(II)配合物与牛血清白蛋白(BSA)的相互作用。利用琼脂盘扩散法研究了希夫碱及其镉配合物对大肠杆菌、霍乱弧菌、肺炎链球菌和蜡样芽孢杆菌等病原菌的抑菌活性。结果表明,由于镉配合物具有较高的亲脂性,其活性高于配体。关键词:镉配合物,牛血清白蛋白相互作用,抗菌活性。
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引用次数: 1
An Ergonomic survey of Health Status of the Handloom Weavers 手摇织机织工健康状况的工效学调查
Pub Date : 2019-02-28 DOI: 10.26438/ijsrbs/v6i1.196202
Santu Durlov, Atanu Saha, Sasangbaha Mandi, S. Sahu
Weaving is a one of the time honored crafts in the globe. The handloom industry is mainly a rural based cottage industry, where the processes are done mainly manually. Some handloom product of West Bengal like “Sari” (a traditional Indian dress worn by females in India) appreciated worldwide. It is an extensive piece of art work exclusively done manually by the weavers. The product development encompasses a wide range of tasks such as manual sorting of raw materials, dyeing, cutting, starching and adjustment of loom, arrangement of thread and spindle insertion, etc. This study was aimed at evaluating the different health problems and socio-economic conditions of the handloom weavers. Three hundred forty (340) weavers were randomly taken from different districts of West Bengal and 108 people from the same localities and similar socioeconomic background were also taken as a referent. Detailed job profile of handloom weavers was also analyzed with the help of established standard questionnaire. It was observed that all types of weavers belong to low socio-economic condition and suffered from several health problems like pain in different body parts, eye constraint, respiratory problems and many more to say.
编织是世界上历史最悠久的工艺之一。手工纺织业主要是一种以农村为基础的家庭手工业,其过程主要是手工完成的。西孟加拉邦的一些手工产品,如“纱丽”(印度女性穿的传统印度服装),在全世界都很受欢迎。这是一件广泛的艺术品,完全由织工手工完成。产品的开发包含了广泛的任务,如手工分拣原料,染色,切割,上浆和调整织机,安排线和插入主轴等。本研究旨在评估手织布机织工的不同健康问题和社会经济状况。从西孟加拉邦的不同地区随机抽取340名织布工,从相同地区和相似社会经济背景中抽取108人作为参考。通过编制标准问卷,详细分析了手织工的工作概况。据观察,所有类型的织布工都属于社会经济条件较低的人,并患有几种健康问题,如身体不同部位疼痛、眼睛受限、呼吸问题等等。
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引用次数: 3
期刊
International Journal of Scientific Research in Biological Sciences
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