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Study on Estimation and Antioxidant activity of Gloriosa superba L. Whole Plant Extract 金凤花全植物提取物的评价及抗氧化活性研究
Pub Date : 2019-06-30 DOI: 10.26438/ijsrbs/v6i3.5055
U. Jothi, J. Jebamalar, T. Sivakumar
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引用次数: 4
Quality evaluation of Coconut Based Snack during storage period 椰子类零食储藏期质量评价
Pub Date : 2019-06-30 DOI: 10.26438/ijsrbs/v6i3.105113
M. Sivasakthi, N. Sangeetha
: Natural versatile ingredients packed with potent nutrients such as beet root, carrot, ginger and mint have been selected for the recent study for their variable vast phenolic compounds and other functional properties as osmotic infusions in the form of filtrates. Sliced samples (0.8±0.1mm) of matured coconuts were subjected to the process osmotic dehydration from 0 min to 720 min at room temperature. Then the slices were dried in a hot-air oven at temperature 45-60°C for about 6-7 hours and freeze drying at temperature (-40 to 30°C) for a period of 14-16 hours. Osmotic medium without the infusion of filtrates of functional ingredients serves as the control. The snack samples were packed using Aluminium foil laminated LDPE pouches with infusion of 100% nitrogen gas composition and stored at ambient temperature till analyzing the shelf life features like free fatty acids, peroxide value, total sugars, moisture content and microbial growth and the results were interpreted using SPSS tool t test ( p≤0.05 ). Upon three months of storage study, the products quality remained good, where as slight deterioration of shelf life characteristics seen beyond three months, showed the keeping quality of the snack.
最近的研究选择了含有甜菜根、胡萝卜、生姜和薄荷等营养成分的天然多功能成分,因为它们具有丰富的酚类化合物和其他功能特性,可以渗透注入滤液。成熟椰子切片样品(0.8±0.1mm)在室温下进行0 ~ 720 min的过程渗透脱水。然后在温度45-60°C的热风烘箱中干燥约6-7小时,在温度(-40 ~ 30°C)下冷冻干燥14-16小时。以渗透性介质为对照,不输注功能成分滤液。零食样品采用铝箔层压LDPE袋包装,注入100%氮气成分,室温保存,分析游离脂肪酸、过氧化值、总糖、水分含量、微生物生长等保质期特征,并采用SPSS工具进行t检验(p≤0.05)。经过3个月的贮藏研究,产品质量保持良好,超过3个月的货架期特征略有恶化,说明零食的保存质量良好。
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引用次数: 0
Cytotoxic effects and Identification of bioactive metabolites from crude extract of potential probiotic bacterium, Bacillus amyloliquefacians CS4, isolated from the gut of freshwater fish Channa striata (Bloch, 1793) 淡水鱼肠道潜在益生菌解淀粉芽孢杆菌CS4粗提物的细胞毒作用及生物活性代谢物鉴定(Bloch, 1793)
Pub Date : 2019-06-30 DOI: 10.26438/ijsrbs/v6i3.1419
R NobelSuryaPandiDurai, D. Aiswarya, Amutha, G. Sethuraman, P. erumal
Received: 23/May/2019, Accepted: 15/Jun/2019, Online: 30/Jun/2019 Abstract— In the present study, the crude secondary metabolites were extracted from the potential probiotic bacterium, Bacillus amyloliquefacians CS4 using solvent ethyl acetate and the cytotoxicity of bacterial extract was assessed against HeLa cell line through MTT assay. The crude extract exhibited cytotoxicity effect on HeLa cell lines with the effective half maximal inhibitory concentration (IC50) value of 121.65μg/ml. The brine shrimp toxicity assay of crude extract revealed half maximal lethal concentration (LC50) value at 93.377μg/ml against Artemia salina. The zebra fish (Danio rerio) embryos were treated with different concentrations of extract upto 72 hpf and was found lowest survivability (36.6 %) at 200μg/ml concentration. The bioactive compounds and their functional groups of crude extract were identified using GC-MS and FTIR analyses. The secondary metabolites produced by B. amyloliquefacians CS4 have caused potential cytotoxic effects on cancer cell line and these compounds could be possibly used for the development of therapeutic agents, in relation to cancer drug discovery.
摘要/ Abstract摘要:本研究以潜在的益生菌解淀粉芽孢杆菌CS4为原料,采用乙酸乙酯溶剂提取其粗次生代谢物,并通过MTT法评价其对HeLa细胞株的细胞毒性。粗提物对HeLa细胞株具有细胞毒作用,有效半最大抑制浓度(IC50)为121.65μg/ml。粗提物对盐蒿的半数最大致死浓度(LC50)为93.377μg/ml。不同浓度的提取液处理斑马鱼胚胎(Danio rerio),浓度为72 hpf, 200μg/ml时存活率最低,为36.6%。采用气相色谱-质谱和红外光谱对粗提物的活性成分及其官能团进行了鉴定。解淀粉芽孢杆菌CS4产生的次生代谢产物对癌细胞具有潜在的细胞毒性作用,这些化合物可能用于开发治疗药物,与癌症药物的发现有关。
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引用次数: 0
Observation of Aqua-Bio fertilizer nutrients influence of Trigonella foenum- graecum L. growth in mediated Aquaponics system for food security. 水-生物肥料养分对水培系统中三角Trigonella foenum- graecum L.生长影响的观察
Pub Date : 2019-04-30 DOI: 10.26438/ijsrbs/v6i2.102110
Manjula., Raja
Received: 21/Mar/2019, Accepted: 10/Apr/2019, Online: 30/Apr/2019 AbstractThe conception of Aquaponics is a synergistic combination of aquaculture and hydroponics that nurture plants with aquaculture water. It is the internationally improved agricultural practices, are needed to assuage rural poverty and enhance food security. Aquaponics is a soil-less combined approach to proficient and sustainable intensification of agriculture that meets the needs of water scarcity inventiveness and generated modern farming practices in agriculture. The nutrient-rich fish water from the fish tanks is used to fertigate hydroponic production beds. To improve the production efficiency can be used bio fertilizers (Aquaponic water and Gracilaria corticata extract) as a liquid it contains organic macro, micronutrient and growth hormones. Tilapia fish produced the nutrient that is utilized by the plant Trigonella foenumgraecum L. The Physico-chemical parameter of both bio fertilizers should be assessed by using the standard procedures. The fish effluent water is compared with the Gracilaria corticata red seaweed extract by foliar spray and combining with both liquid bio fertilizers (Aquaponic water and seaweed extracts). Morphological growths of the plants were assessed. The combination of Aquaponic water and Gracilaria corticata seaweed extract was given the good result, but the estimation (15.26± 0.29, 24.06± 0.27, 30± 0.64, 35.94 ±0.28 cm plant height at 7, 14, 21 and 28 DAS) (2.6± 0.1 branches at 30, DAS) (533.67 ±11and 51 ± 1 mg shoot and root fresh weight per plant) leaf area (46.62±0.89cm2) at 28 DAS was related to the Aquaponic water in the plant Trigonella foenumgraecum L. Thus, the present result revealed that the integrated aquaponic system is the new agricultural farming systems with liquid bio fertilizers are ecofriendly practice for food security.
摘要Aquaponics的概念是水产养殖和水培的协同结合,用水产养殖的水来培育植物。国际上需要改进农业做法,以减轻农村贫困和加强粮食安全。鱼菜共生是一种无土综合方法,可实现熟练和可持续的农业集约化,满足水资源短缺的需求,具有创造性,并产生了现代农业实践。养鱼池中富含营养的鱼水用于水培生产床的施肥。为提高生产效率,可采用含有机宏量、微量营养素和生长激素的生物肥料(水培水和江蓠提取物)作为液体。罗非鱼产生的营养物质为葫芦巴植物所利用。这两种生物肥料的理化参数应按标准程序进行评估。采用叶面喷施和混合使用液体生物肥料(水培水和海藻提取物)的方法,对鱼排水和紫菜提取物进行了比较。对植物的形态生长进行了评价。水培水与海苔草提取物联合使用效果较好,但在7、14、21和28 DAS时的株高(15.26±0.29、24.06±0.27、30±0.64、35.94±0.28 cm)(30、DAS时的枝重2.6±0.1 mg)(533.67±11和51±1 mg茎根鲜重)(28 DAS时的叶面积(46.62±0.89cm2)与三角藻的水培水有关。综上所述,综合水培系统是一种新型的液体生物肥料农业系统,是保障粮食安全的环保实践。
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引用次数: 0
Understanding the impact of Bacillus thuringiensis proteins on non-target organisms 了解苏云金芽孢杆菌蛋白对非靶生物的影响
Pub Date : 2019-04-30 DOI: 10.26438/ijsrbs/v6i2.169176
T. Singh, Y. Tiwari, G. Awasthi
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引用次数: 1
Zinc oxide (ZnO) induced toxicity and behavioural changes to oligochaete worm Tubifex tubifex (Muller) 氧化锌(ZnO)诱导的少毛线虫的毒性和行为改变(Muller)
Pub Date : 2019-04-30 DOI: 10.26438/ijsrbs/v6i2.3542
A. Chatterjee, Ritwick Bhattacharya, N. Saha
Zinc is among the important trace element of the body by participating in several biological processes. But it shows toxicity after a certain concentration. Zinc oxide (ZnO), a hazardous heavy metal, is frequently used in both bulk and nanoparticle format in industrial and household purposes throughout India. In the present study the 24, 48, 72 and 96h LC50 values of ZnO was monitored to benthic oligochaete worm Tubifex tubifex (Muller) through Finney’s probit analysis method. Different abnormal behavioural changes in the worm were also recorded during the exposure period. The 24, 48, 72 and 96h LC50 values of Zinc oxide (ZnO) to Tubifex tubifex was obtained as 30.88, 24.15, 19.95 and 11.15 mg/l respectively. The mortality rate of Tubifex tubifex varied significantly (p<0.05) with a dose and time dependent manner (24, 48, 72 and 96 h). The exposed worm showed several erratic behaviors with the gradually increasing concentrations of the metal and with the progress of time of exposure. The safe concentration of this metal was ranged between 0.0001115 -1.11 mg/l. Based on 96h LC50 value the Maximum allowable concentration (MAC), no observable effect concentration (NOEC) and the lowest observable effect concentration (LOEC) of Zinc oxide to Tubifex tubifex were 1.11, 1.3 and 2.1 mg/l respectively. The present study will be useful in determination of the safe dose of the toxicant before its disposal to the environment.
锌是人体重要的微量元素之一,参与多种生物过程。但在一定浓度后显示出毒性。氧化锌(ZnO)是一种危险的重金属,在印度各地的工业和家庭用途中经常以散装和纳米颗粒的形式使用。本研究采用Finney概率分析法,对底栖寡栖蠕虫Tubifex Tubifex (Muller)的24、48、72和96h的LC50值进行了监测。在暴露期间,还记录了蠕虫的不同异常行为变化。结果表明,氧化锌(ZnO)对Tubifex的LC50值分别为30.88、24.15、19.95和11.15 mg/l。在24、48、72和96 h时,管翅小虫的死亡率呈剂量依赖性和时间依赖性(p<0.05),随着金属浓度的逐渐增加和暴露时间的延长,暴露后的虫体表现出一些不稳定的行为。这种金属的安全浓度范围在0.0001115 -1.11毫克/升之间。以96h LC50值为基准,氧化锌对管翅虫的最大允许浓度(MAC)为1.11,无观察效应浓度(NOEC)为1.3,最低观察效应浓度(LOEC)为2.1 mg/l。本研究将有助于确定该毒物在排入环境之前的安全剂量。
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引用次数: 4
Morphological, Ethno biological and Phytopharmacological Attributes of Tridax procumbensLinn. (Asteraceae) A Review 原春三叶的形态、民族生物学和植物药理特性。(菊科)综述
Pub Date : 2019-04-30 DOI: 10.26438/ijsrbs/v6i2.182191
P. Ghosh, Swagata Biswas, Maitrayee Biswas, A. Dutta, S. Sil, Sirshendu Chatterjee
Tridax procumbens Linn. is an widespread herbaceous weed distributed throughout India. It is commonly known as coat buttons, is also dispensed as Bhringraj, and used as Ayurvedic medicine for liver disorders and hair growth. The plant is an annual or perennial herbaceous weed, found mainly in the croplands, disturbed areas, lawn, and roadsides. Literature survey suggested that the various parts of the plant were reported to possess phytochemical compounds like alkaloids, carotenoids, flavonoids, saponins, fumaric acid, β-sitosterol, and tannins. It was richly endowed with oleanolic acid, and high content of sodium and potassium are also present. Bioactive components like luteolin, glucoluteolin, quercetin, and isoquercetin also have been reported from its flowers. Primary plant pigments such as chlorophyll and carotenoids also analyzed from leaves. So, it is well known for several potential pharmacological activities like anti-microbial, anti-oxidant, wound healing, insecticidal, hepato-protective, anti-diabetic, immune modulating, anti-cancer, anti-dysentery, anti-inflammatory, and anti-hair fall. The present review study deals with natural habitat, taxonomical, phytochemical, pharmacological and toxicological activities of Tridax procumbens. This study may be helpful for identification and preparation of a clear profile of the plant which may open new avenues in the medical field in the treatment of various diseases. KeywordsTridax procumbens, Medicinal Weed, Ethnobotany, Morphological, Phytopharmacology.
三叶草;是一种广泛分布在印度的草本杂草。它通常被称为大衣纽扣,也被称为Bhringraj,并被用作治疗肝脏疾病和头发生长的阿育吠陀药物。该植物是一种一年生或多年生草本杂草,主要生长在农田、干扰区、草坪和路边。文献调查表明,该植物的各个部位都含有生物碱、类胡萝卜素、类黄酮、皂苷、富马酸、β-谷甾醇和单宁等植物化学物质。它富含齐墩果酸,钠和钾含量也很高。生物活性成分如木犀草素、葡萄糖木犀草素、槲皮素和异槲皮素也有报道从其花中提取。叶绿素和类胡萝卜素等初级植物色素也从叶子中进行了分析。因此,它具有抗微生物、抗氧化、伤口愈合、杀虫、保肝、抗糖尿病、调节免疫、抗癌、抗痢疾、抗炎、防脱发等潜在的药理作用。本文对原藜的自然生境、分类、植物化学、药理和毒理学活性等方面进行了综述。本研究可为该植物的鉴定和制备提供参考,为其治疗多种疾病开辟新的途径。【关键词】原春草;药用杂草;民族植物学;
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引用次数: 13
Isolation and Screening of Chlorella Sorokiniana for Wastewater Treatment and Biodiesel Production 小球藻的分离与筛选及其废水处理和生物柴油生产
Pub Date : 2019-04-30 DOI: 10.26438/ijsrbs/v6i2.5967
M. S.S, S. Girisha
- Microalgae grown on wastewater are a probable source of low cost wastewater treatment and biodiesel production. In the study, microalgae was enumerated and identified as C hlorella sorokiniana by 18S rDNA sequence which was cultivated in different wastewater for nutrient removal as well as biodiesel production were studied. The results reveal that the pH of different wastewater samples almost neutralized by microalgae, the total dissolved solids elimination ranging from 52 to 66%, the biological oxygen demand removal efficiency varied much among different wastewaters the removal rate is of 72 to 90%, the chemical oxygen demand removal ranges from 59 to 75%. The magnesium level was decreased and efficiency is about 36 to 60%, the sulphates absorption efficiency was ranging from 57 to 68% and the chloride removal efficiency was 13 to 33%. The lipid content was obtained from the algal biomass which are grown in different wastewater samples was transesterified for biodiesel production, the biodiesel was analyzed by FTIR which meets the ASTM and EU standards, hence from the current study it is evident that Chlorella sorokiniana can be effectively used for potential source for phycoremediation and biodiesel production.
-在废水上生长的微藻是低成本废水处理和生物柴油生产的可能来源。在这项研究中,微藻列举并确认为C hlorella sorokiniana 18 s rDNA序列在不同的养分去除废水培养以及生物柴油的生产进行了研究。结果表明,微藻对不同废水样品的pH值基本中和,对总溶解固体去除率在52% ~ 66%之间,生物需氧量去除率在72% ~ 90%之间,化学需氧量去除率在59% ~ 75%之间。镁含量降低,效率约为36 ~ 60%,硫酸盐吸附效率为57 ~ 68%,氯化物去除效率为13 ~ 33%。通过对不同废水样品中生长的藻类生物质的脂质含量进行酯交换制备生物柴油,并对生物柴油进行FTIR分析,所得生物柴油符合ASTM和EU标准,因此从目前的研究中可以看出,小球藻可以有效地作为生物修复和生物柴油生产的潜在来源。
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引用次数: 0
Comprehend of Patho-physiological Changes and Consequential Prognosis in Marmabhighata (Injury to Marma) 对Marmabhighata (Marma损伤)病理生理变化及预后的认识
Pub Date : 2019-04-30 DOI: 10.26438/ijsrbs/v6i2.160168
Hiarman Banjare, S. Meher, Sachinkumar Baghel, S. Dalai
„Marma‟ literary communicates the sense of vital parts of the body. The science of Marma is an unique approach of Ayurveda and established to help the practice of surgery. There are total 107 Marma‟s in the human body. These are classified into five types according to location, structures involved, effect and consequences or prognosis of the trauma over there. Any type of Marmabhighata (injury to Marma) is likely to produce subjective manifestations governed by the extent and the nature of injuries to the Marmas. Tridosha in the body have their Panchabhautik origin. The Pancha mahabhautas are the main constituting component of Tridosha such that, the consequence of traumatised Marma has got direct bearing on the predominance of Tridoshas over there. Any trauma to these Marmas is likely to provoke both the Shareerik and Manasik dosha which thereby affects the body (Sharira) and Mind (Mana). So, as the base (body and Mind) gets affected, the Jeevatma or Bhootatma itself ceases to exit. This changes in Marma after injury such as deformities and delayed healing mainly depends upon the consequences that occur due to patho-physiological changes over Marma. Keyword: Marma, Marmabhighata, injury to Marma, Tridosha, Panchabhuta, Consequences
“玛玛”文学传达了身体重要部位的感觉。玛玛科学是阿育吠陀的一种独特方法,旨在帮助外科手术的实践。人体内总共有107个马尔马。根据位置,涉及的结构,影响和后果以及创伤的预后可以分为五种类型。任何类型的Marmabhighata(对Marma的伤害)都可能产生主观表现,这取决于对Marma伤害的程度和性质。身体中的Tridosha有它们的Panchabhautik起源。Pancha mahabhautas是Tridosha的主要组成部分,因此,受到创伤的Marma的后果直接影响了Tridosha在那里的主导地位。对这些Marmas的任何创伤都可能同时激发Shareerik和Manasik dosha,从而影响身体(Sharira)和心灵(Mana)。因此,当基础(身心)受到影响时,菩提摩(Jeevatma)或菩提摩(Bhootatma)本身停止退出。损伤后Marma的变化,如畸形和延迟愈合,主要取决于Marma的病理生理变化所产生的后果。关键词:Marma, Marmabhighata, Marma损伤,Tridosha, Panchabhuta,后果
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引用次数: 0
Spatio-Temporal Variations in Mangrove Vegetation in conjunction with Related Environmental Factors in Pichavaram (India) 1996-2016 印度Pichavaram地区红树林植被时空变化及其相关环境因子研究(英文版)1996-2016
Pub Date : 2019-04-30 DOI: 10.26438/ijsrbs/v6i2.1525
ripa M K, Ashwini N. Mudaliar, N. Lele, A. Mankad, T. Murthy
The major objective of the study is to explore the inter-annual vegetation changes of Pichavaram mangroves over two decades (from 1996 to 2016). The study mainly focuses on the summer and onset monsoon season, where the variability in vegetation is easily noticed. Vegetation indices like Normalized Difference Vegetation Index (NDVI), Land Surface Temperature (LST) and Land Surface Water Index (LSWI) were estimated from the remotely sensed data of Landsat imagery. A comprehensive evaluation of the relationship among the vegetation indices reveals that there is an increase in the area occupied by the mangroves throughout the years. Correspondingly, the area under the mangrove cover have also faced an increase in the surface temperature, but much lesser when compared to the adjacent areas. According to the results obtained, NDVI, LSWI, LST, can be used to understand the status of mangrove vegetation with increase and decrease of temperature and water. It is found that the correlation among the remotely sensed indices depend on the season of the year. The summer season in general exhibited higher negative correlation with NDVI and LST and also between LST and LSWI than the onset monsoon season. It is also apparent that with the passing of years, the correlations among the indices are also found to be increasing. Thus in the global scenario of variable climate change, it is important to monitor the seasonal changes in vegetation, especially mangroves, for their effective conservation.
本研究的主要目的是探讨皮查瓦拉姆红树林在1996年至2016年间的年际植被变化。研究主要集中在夏季和季风开始季节,植被的变化很容易被注意到。利用Landsat影像遥感数据估算了归一化植被指数(NDVI)、地表温度(LST)和地表水指数(LSWI)等植被指数。综合评价植被指数间的关系,红树林面积呈逐年增加的趋势。相应地,红树林覆盖下的区域也面临着地表温度的升高,但与邻近区域相比要小得多。结果表明,NDVI、LSWI、LST可用于了解红树林植被随温度和水分的增减状况。结果表明,各遥感指标间的相关性与季节有关。总体而言,夏季与NDVI、LST以及LST与LSWI的负相关高于季风开始期。随着时间的推移,各指数之间的相关性也明显增强。因此,在全球气候变化多变的情况下,监测植被的季节变化,特别是红树林,对其进行有效保护是很重要的。
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引用次数: 2
期刊
International Journal of Scientific Research in Biological Sciences
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