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Description of six novel species Bacillus bruguierae sp. nov., Bacillus kandeliae sp. nov., Bacillus yunxiaonensis sp. nov., Metabacillus rhizosphaerae sp. nov., Metabacillus sediminis sp. nov. and Psychrobacillus mangrovi sp. nov., isolated from mangrove ecosystem.
IF 2 3区 生物学 Q4 MICROBIOLOGY Pub Date : 2025-01-01 DOI: 10.1099/ijsem.0.006656
Guo-Hong Liu, Manik Prabhu Narsing Rao, Huai Shi, Qian-Qian Chen, Syed Raziuddin Quadri, Wen-Jun Li

Six Gram-stain-positive and rod-shaped strains, designated FJAT-51614T, FJAT-51639T, FJAT-52054T, FJAT-52991T, FJAT-53654T and FJAT-53711T, were isolated from a mangrove ecosystem. The condition for growth among the strains varied (pH ranging 5.0-11.0 and temperature 15-45 °C). These strains showed the highest 16S rRNA gene sequence similarity to the genera Bacillus, Metabacillus and Psychrobacillus. Menaquinone MK-7 (100%) was present in all strains except strain FJAT-51614T, which contained MK-7 (32%) and MK-8 (68%). The major fatty acids (>10%) in strains FJAT-52054T and FJAT-51614T were anteiso-C15 : 0 and iso-C14 : 0. The major fatty acid (>10%) in strain FJAT-53654T was anteiso-C15 : 0, while in strain FJAT-51639T were iso-C15 : 0 and iso-C17 : 0. The major fatty acids in strains FJAT-52991T and FJAT-53711T were iso-C15 : 0 and iso-C16 : 0. The major polar lipids in strains FJAT-51639T, FJAT-52991T and FJAT-51614T were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, while strains FJAT-53711T, FJAT-52054T and FJAT-53654T consist of diphosphatidylglycerol and phosphatidylglycerol. The genome-relatedness values among the present study strains and the closest species were below the recognized cutoff levels for species delineation. The polyphasic analysis comparison results represent six strains as novel species for which the names Bacillus bruguierae sp. nov. (FJAT-51639T=GDMCC 1.3073T=JCM 35615T), Bacillus kandeliae sp. nov. (FJAT-52991T=GDMCC 1.3069T=JCM 35619T), Bacillus yunxiaonensis sp. nov. (FJAT-53711T=GDMCC 1.3068T=JCM 35616T), Metabacillus rhizosphaerae sp. nov. (FJAT-53654T=GDMCC 1.3075T=JCM 35614T), Metabacillus sediminis sp. nov. (FJAT-52054T=GDMCC 1.3074T=JCM 35620T) and Psychrobacillus mangrovi sp. nov. (FJAT-51614T=GDMCC 1.3072T=JCM35478T) are proposed.

从红树林生态系统中分离出六株革兰氏染色阳性的杆状菌株,分别命名为 FJAT-51614T、FJAT-51639T、FJAT-52054T、FJAT-52991T、FJAT-53654T 和 FJAT-53711T。这些菌株的生长条件各不相同(pH 值在 5.0-11.0 之间,温度为 15-45 ℃)。这些菌株的 16S rRNA 基因序列与芽孢杆菌属、拟杆菌属和精神杆菌属的相似度最高。除菌株 FJAT-51614T 含有 MK-7(32%)和 MK-8(68%)外,其他菌株均含有 Menaquinone MK-7(100%)。FJAT-52054T 和 FJAT-51614T 菌株中的主要脂肪酸(>10%)是前异-C15 :0 和异-C14 :0.菌株 FJAT-53654T 的主要脂肪酸(>10%)是前异-C15 :0,而在菌株 FJAT-51639T 中则是异-C15 : 0 和异-C17 : 0:0 和异 C17 :0.菌株 FJAT-52991T 和 FJAT-53711T 的主要脂肪酸是异-C15 : 0 和异-C16 : 0:0 和异 C16 :0.菌株 FJAT-51639T、FJAT-52991T 和 FJAT-51614T 的主要极性脂质为二磷脂酰甘油、磷脂酰甘油和磷脂酰乙醇胺,而菌株 FJAT-53711T、FJAT-52054T 和 FJAT-53654T 则由二磷脂酰甘油和磷脂酰甘油组成。本研究菌株与最接近物种之间的基因组相关性值低于公认的物种划分临界值。多相分析比较结果表明,6 株菌株为新种,其名称分别为 Bacillus bruguierae sp.nov.(FJAT-51639T=GDMCC 1.3073T=JCM 35615T)、Bacillus kandeliae sp.nov.(FJAT-52991T=GDMCC 1.3069T=JCM 35619T)、Bacillus yunxiaonensis sp.nov.(FJAT-53711T=GDMCC 1.3069T=JCM 35619T)、Bacillus yunxiaonensis sp.(FJAT-53711T=GDMCC 1.3068T=JCM 35616T), Metabacillus rhizosphaerae sp.nov.(FJAT-52054T=GDMCC 1.3074T=JCM 35620T)和 Psychrobacillus mangrovi sp.
{"title":"Description of six novel species <i>Bacillus bruguierae</i> sp. nov., <i>Bacillus kandeliae</i> sp. nov., <i>Bacillus yunxiaonensis</i> sp. nov., <i>Metabacillus rhizosphaerae</i> sp. nov., <i>Metabacillus sediminis</i> sp. nov. and <i>Psychrobacillus mangrovi</i> sp. nov., isolated from mangrove ecosystem.","authors":"Guo-Hong Liu, Manik Prabhu Narsing Rao, Huai Shi, Qian-Qian Chen, Syed Raziuddin Quadri, Wen-Jun Li","doi":"10.1099/ijsem.0.006656","DOIUrl":"10.1099/ijsem.0.006656","url":null,"abstract":"<p><p>Six Gram-stain-positive and rod-shaped strains, designated FJAT-51614<sup>T</sup>, FJAT-51639<sup>T</sup>, FJAT-52054<sup>T</sup>, FJAT-52991<sup>T</sup>, FJAT-53654<sup>T</sup> and FJAT-53711<sup>T</sup>, were isolated from a mangrove ecosystem. The condition for growth among the strains varied (pH ranging 5.0-11.0 and temperature 15-45 °C). These strains showed the highest 16S rRNA gene sequence similarity to the genera <i>Bacillus</i>, <i>Metabacillus</i> and <i>Psychrobacillus</i>. Menaquinone MK-7 (100%) was present in all strains except strain FJAT-51614<sup>T</sup>, which contained MK-7 (32%) and MK-8 (68%). The major fatty acids (>10%) in strains FJAT-52054<sup>T</sup> and FJAT-51614<sup>T</sup> were anteiso-C<sub>15 : 0</sub> and iso-C<sub>14 : 0</sub>. The major fatty acid (>10%) in strain FJAT-53654<sup>T</sup> was anteiso-C<sub>15 : 0</sub>, while in strain FJAT-51639<sup>T</sup> were iso-C<sub>15 : 0</sub> and iso-C<sub>17 : 0</sub>. The major fatty acids in strains FJAT-52991<sup>T</sup> and FJAT-53711<sup>T</sup> were iso-C<sub>15 : 0</sub> and iso-C<sub>16 : 0</sub>. The major polar lipids in strains FJAT-51639<sup>T</sup>, FJAT-52991<sup>T</sup> and FJAT-51614<sup>T</sup> were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, while strains FJAT-53711<sup>T</sup>, FJAT-52054<sup>T</sup> and FJAT-53654<sup>T</sup> consist of diphosphatidylglycerol and phosphatidylglycerol. The genome-relatedness values among the present study strains and the closest species were below the recognized cutoff levels for species delineation. The polyphasic analysis comparison results represent six strains as novel species for which the names <i>Bacillus bruguierae</i> sp. nov. (FJAT-51639<sup>T</sup>=GDMCC 1.3073<sup>T</sup>=JCM 35615<sup>T</sup>), <i>Bacillus kandeliae</i> sp. nov. (FJAT-52991<sup>T</sup>=GDMCC 1.3069<sup>T</sup>=JCM 35619<sup>T</sup>), <i>Bacillus yunxiaonensis</i> sp. nov. (FJAT-53711<sup>T</sup>=GDMCC 1.3068<sup>T</sup>=JCM 35616<sup>T</sup>), <i>Metabacillus rhizosphaerae</i> sp. nov. (FJAT-53654<sup>T</sup>=GDMCC 1.3075<sup>T</sup>=JCM 35614<sup>T</sup>), <i>Metabacillus sediminis</i> sp. nov. (FJAT-52054<sup>T</sup>=GDMCC 1.3074<sup>T</sup>=JCM 35620<sup>T</sup>) and <i>Psychrobacillus mangrovi</i> sp. nov. (FJAT-51614<sup>T</sup>=GDMCC 1.3072<sup>T</sup>=JCM35478<sup>T</sup>) are proposed.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Flavobacterium arundinis sp. nov., Flavobacterium calami sp. nov., Flavobacterium helocola sp. nov. and Flavobacterium flavipallidum sp. nov., isolated from stagnant water in a clump of Phragmites japonica Steud.
IF 2 3区 生物学 Q4 MICROBIOLOGY Pub Date : 2025-01-01 DOI: 10.1099/ijsem.0.006667
Sunho Park, Inhyup Kim, Haejin Woo, Hyunji Lee, Subin Yook, Taegun Seo

Four novel bacterial strains were isolated from stagnant water in a clump of Phragmites japonica Steud. The four Gram-negative, facultatively anaerobic, rod-shaped, non-motile and yellow-coloured strains were designated as DGU11T, DGU38T, DGU41T and DGU99T. The four novel strains exhibited 16S rRNA gene similarities with their closest type strains, ranging from 95.7 to 98.7%, average nucleotide identity values from 75.3 to 95.6% and digital DNA-DNA hybridization values between 17.9 and 59.6%. The cells of strain DGU11T were non-motile and grew at 10-30°C (optimum 25 °C), a pH range of 4.0-12.0 (optimum 9.0) and in the presence of 0-1.0% sodium chloride (NaCl) (optimum 0%). The cells of strain DGU38T were non-motile and grew at 10-35°C (optimum 30 °C), a pH range of 4.0-8.0 (optimum 7.0) and at 0 -2.0% NaCl (optimum 0%). The cells of strain DGU41T were non-motile and grew at 15-50°C (optimum 30 °C), a pH range of 6.0-9.0 (optimum 7.0) and in the presence of 0% NaCl (optimum 0%). The cells of strain DGU99T were non-motile and grew at 15-35°C (optimum 25 °C), a pH range of 6.0-12.0 (optimum 9.0) and in the presence of 0 -2.0% NaCl (optimum 0%). The major fatty acid composition of the four novel strains was iso-C15:0 and C16:1  ω7c/C16:1  ω6c, which is similar to other Flavobacterium species. The proposed names of the novel strains are Flavobacterium arundinis sp. nov. (type strain DGU11T=KACC 23722T=TBRC 19004T), Flavobacterium calami sp. nov. (type strain DGU38T=KACC 23723T=TBRC 19005T), Flavobacterium helocola sp. nov. (type strain DGU41T=KACC 23724T=TBRC 19006T) and Flavobacterium flavipallidum sp. nov. (type strain DGU99T=KACC 23725T=TBRC 19007T).

{"title":"<i>Flavobacterium arundinis</i> sp. nov., <i>Flavobacterium calami</i> sp. nov., <i>Flavobacterium helocola</i> sp. nov. and <i>Flavobacterium flavipallidum</i> sp. nov., isolated from stagnant water in a clump of <i>Phragmites japonica</i> Steud.","authors":"Sunho Park, Inhyup Kim, Haejin Woo, Hyunji Lee, Subin Yook, Taegun Seo","doi":"10.1099/ijsem.0.006667","DOIUrl":"https://doi.org/10.1099/ijsem.0.006667","url":null,"abstract":"<p><p>Four novel bacterial strains were isolated from stagnant water in a clump of <i>Phragmites japonica</i> Steud. The four Gram-negative, facultatively anaerobic, rod-shaped, non-motile and yellow-coloured strains were designated as DGU11<sup>T</sup>, DGU38<sup>T</sup>, DGU41<sup>T</sup> and DGU99<sup>T</sup>. The four novel strains exhibited 16S rRNA gene similarities with their closest type strains, ranging from 95.7 to 98.7%, average nucleotide identity values from 75.3 to 95.6% and digital DNA-DNA hybridization values between 17.9 and 59.6%. The cells of strain DGU11<sup>T</sup> were non-motile and grew at 10-30°C (optimum 25 °C), a pH range of 4.0-12.0 (optimum 9.0) and in the presence of 0-1.0% sodium chloride (NaCl) (optimum 0%). The cells of strain DGU38<sup>T</sup> were non-motile and grew at 10-35°C (optimum 30 °C), a pH range of 4.0-8.0 (optimum 7.0) and at 0 -2.0% NaCl (optimum 0%). The cells of strain DGU41<sup>T</sup> were non-motile and grew at 15-50°C (optimum 30 °C), a pH range of 6.0-9.0 (optimum 7.0) and in the presence of 0% NaCl (optimum 0%). The cells of strain DGU99<sup>T</sup> were non-motile and grew at 15-35°C (optimum 25 °C), a pH range of 6.0-12.0 (optimum 9.0) and in the presence of 0 -2.0% NaCl (optimum 0%). The major fatty acid composition of the four novel strains was iso-C<sub>15:0</sub> and C<sub>16:1</sub> <i> ω7</i>c<i>/</i>C1<sub>6:1</sub> <i> ω6</i>c, which is similar to other <i>Flavobacterium</i> species. The proposed names of the novel strains are <i>Flavobacterium arundinis</i> sp. nov. (type strain DGU11<sup>T</sup>=KACC 23722<sup>T</sup>=TBRC 19004<sup>T</sup>), <i>Flavobacterium calami</i> sp. nov. (type strain DGU38<sup>T</sup>=KACC 23723<sup>T</sup>=TBRC 19005<sup>T</sup>), <i>Flavobacterium helocola</i> sp. nov. (type strain DGU41<sup>T</sup>=KACC 23724<sup>T</sup>=TBRC 19006<sup>T</sup>) and <i>Flavobacterium flavipallidum</i> sp. nov. (type strain DGU99<sup>T</sup>=KACC 23725<sup>T</sup>=TBRC 19007<sup>T</sup>).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pseudomonas spirodelae, sp. nov., a bacterium isolated from the roots of the aquatic plant Spirodela polyrhiza. 螺旋体假单胞菌,sp. nov.,一种从水生植物多螺旋体根中分离出来的细菌。
IF 2 3区 生物学 Q4 MICROBIOLOGY Pub Date : 2025-01-01 DOI: 10.1099/ijsem.0.006637
Karlie Vandeborne, Nathan Guy, George Lengyel, Jonathan Franks, Chris Maltman

A polyphasic taxonomic study was carried out on strain T5W1T, isolated from the roots of the aquatic plant Spirodela polyrhiza. This isolate is Gram-negative, rod-shaped, motile, aerobic and non-pigmented. Nearly complete 16S rRNA gene sequence homology related the strain to Pseudomonas, with 98.4% similarity to P. guineae, P. peli and P. leptonychotis. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) with the closest phylogenetic neighbour of T5W1T showed differences at the species level, further confirmed by differences in several physiological characteristics. The main fatty acids are summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c), C18 : 1 ω7c, and C16 : 0. The DNA G+C content is 59.3 mol%. Q-9 is the primary ubiquinone found, and phosphatidylethanolamine is the dominant polar lipid, with lesser amounts of phosphatidylglycerol, diphosphatidylglycerol and phosphatidylserine. Based on the results obtained, this bacterium is assigned to the genus Pseudomonas as a new species with the name Pseudomonas spirodelae sp. nov., type strain T5W1T (=NRRL B-65714T =DSM 118085T).

对从水生植物多根螺旋体(Spirodela polyrhiza)根中分离的菌株T5W1T进行了多相分类研究。革兰氏阴性,杆状,可运动,需氧,无色素。该菌株16S rRNA基因序列与假单胞菌同源性接近,与几内亚假单胞菌、peli假单胞菌和leptonychotis假单胞菌相似度为98.4%。与T5W1T最接近的系统发育邻居的平均核苷酸同一性(ANI)和数字DNA-DNA杂交(dDDH)在物种水平上显示出差异,进一步证实了几个生理特征的差异。主要脂肪酸归纳为特征3 (C16: 1 ω7c和/或C16: 1 ω6c)、C18: 1 ω7c和C16: 0。DNA G+C含量为59.3 mol%。Q-9是主要的泛醌,磷脂酰乙醇胺是主要的极性脂质,还有少量的磷脂酰甘油、二磷脂酰甘油和磷脂酰丝氨酸。根据上述结果,将该细菌划归为假单胞菌属新种,命名为Pseudomonas spirrodelae sp. nov.,型菌株T5W1T (=NRRL B-65714T =DSM 118085T)。
{"title":"<i>Pseudomonas spirodelae,</i> sp. nov., a bacterium isolated from the roots of the aquatic plant <i>Spirodela polyrhiza</i>.","authors":"Karlie Vandeborne, Nathan Guy, George Lengyel, Jonathan Franks, Chris Maltman","doi":"10.1099/ijsem.0.006637","DOIUrl":"https://doi.org/10.1099/ijsem.0.006637","url":null,"abstract":"<p><p>A polyphasic taxonomic study was carried out on strain T5W1<sup>T</sup>, isolated from the roots of the aquatic plant <i>Spirodela polyrhiza</i>. This isolate is Gram-negative, rod-shaped, motile, aerobic and non-pigmented. Nearly complete 16S rRNA gene sequence homology related the strain to <i>Pseudomonas</i>, with 98.4% similarity to <i>P. guineae</i>, <i>P. peli</i> and <i>P. leptonychotis</i>. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) with the closest phylogenetic neighbour of T5W1<sup>T</sup> showed differences at the species level, further confirmed by differences in several physiological characteristics. The main fatty acids are summed feature 3 (C<sub>16 : 1</sub> <i> </i> <sub>ω7c</sub> and/or C<sub>16 : 1</sub> <i> </i> <sub>ω6c</sub>), C<sub>18 : 1</sub> <i> </i> <sub>ω7c</sub>, and C<sub>16 : 0</sub>. The DNA G+C content is 59.3 mol%. Q-9 is the primary ubiquinone found, and phosphatidylethanolamine is the dominant polar lipid, with lesser amounts of phosphatidylglycerol, diphosphatidylglycerol and phosphatidylserine. Based on the results obtained, this bacterium is assigned to the genus <i>Pseudomonas</i> as a new species with the name <i>Pseudomonas spirodelae</i> sp. nov., type strain T5W1<sup>T</sup> (=NRRL B-65714<sup>T</sup> =DSM 118085<sup>T</sup>).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142962156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Four novel nontuberculous mycobacteria species: Mycobacterium wendilense sp. nov., Mycobacterium burgundiense sp. nov., Mycobacterium kokjensenii sp. nov. and Mycobacterium holstebronense sp. nov. revived from a historical Danish strain collection. 四种新的非结核分枝杆菌:文迪氏分枝杆菌,勃艮第分枝杆菌,kokjensenii分枝杆菌和holstebronense分枝杆菌,从丹麦的历史菌株收集中复活。
IF 2 3区 生物学 Q4 MICROBIOLOGY Pub Date : 2025-01-01 DOI: 10.1099/ijsem.0.006620
Xenia Emilie Sinding Iversen, Erik Michael Rasmussen, Dorte Bek Folkvardsen, Erik Svensson, Conor J Meehan, René Jørgensen, Anders Norman, Troels Lillebaek

Four novel nontuberculous mycobacteria were discovered from a historical strain collection at the International Reference Laboratory of Mycobacteriology at Statens Serum Institut in Copenhagen, Denmark. Phylogenetic analysis combining the 16S rrs, internal transcribed spacer and 23S rrl elements, as well as a single-copy core-gene (hsp65, rpoB+C, secA and tuf) analysis of these freeze-dried mycobacteria, clinically isolated from gastric lavage samples between 1948 and 1955, showed to be associated with type strains grouping within the Terra and Fortuitum-Vaccae clade. Phenotypic characteristics, biochemical properties and fatty acid and mycolic acid profiles supported the classification as novel strains. A genomic comparison to the closest related type strain was done by calculating average nucleotide identity and in silico DNA:DNA hybridization values, which showed 87.9% and 33.0% for Mu0050, 85.2% and 27.4% for Mu0053, 85.3% and 27.6% for Mu0083 and 93.3% and 50.1% for Mu0102, respectively. The names proposed for the new species are Mycobacterium wendilense sp. nov. (Mu0050T=ITM 501390T=CCUG 77525T), Mycobacterium burgundiense sp. nov. (Mu0053T=ITM 501391T=CCUG 77526T), Mycobacterium kokjensenii sp. nov. (Mu0083T=ITM 501392T=CCUG 77527T) and Mycobacterium holstebronense sp. nov. (Mu0102T=ITM 501393T=CCUG 77528T).

在丹麦哥本哈根Statens血清研究所国际分枝杆菌学参考实验室的历史菌株收集中发现了四种新的非结核分枝杆菌。对1948 ~ 1955年间临床从胃灌洗液中分离的冻干分枝杆菌进行了16S rrs、内部转录间隔段和23S rrl元件以及一个单拷贝核心基因(hsp65、rpoB+C、secA和tuf)的系统发育分析,结果表明,这些分枝杆菌与Terra和Fortuitum-Vaccae分支的类型菌株分组有关。表型特征、生化特性以及脂肪酸和霉菌酸谱支持其分类为新菌株。结果表明,Mu0050、Mu0053、Mu0083和Mu0102分别为87.9%和33.0%、85.2%和27.4%、85.3%和27.6%、93.3%和50.1%。提出的新种名称为温氏分枝杆菌sp. 11 (Mu0050T=ITM 501390T=CCUG 77525T)、勃艮第分枝杆菌sp. 11 (Mu0053T=ITM 501391T=CCUG 77526T)、柯延森分枝杆菌sp. 11 (Mu0083T=ITM 501392T=CCUG 77527T)和霍尔斯特bronense分枝杆菌sp. 11 (Mu0102T=ITM 501393T=CCUG 77528T)。
{"title":"Four novel nontuberculous mycobacteria species: <i>Mycobacterium wendilense</i> sp. nov., <i>Mycobacterium burgundiense</i> sp. nov., <i>Mycobacterium kokjensenii</i> sp. nov. and <i>Mycobacterium holstebronense</i> sp. nov. revived from a historical Danish strain collection.","authors":"Xenia Emilie Sinding Iversen, Erik Michael Rasmussen, Dorte Bek Folkvardsen, Erik Svensson, Conor J Meehan, René Jørgensen, Anders Norman, Troels Lillebaek","doi":"10.1099/ijsem.0.006620","DOIUrl":"https://doi.org/10.1099/ijsem.0.006620","url":null,"abstract":"<p><p>Four novel nontuberculous mycobacteria were discovered from a historical strain collection at the International Reference Laboratory of Mycobacteriology at Statens Serum Institut in Copenhagen, Denmark. Phylogenetic analysis combining the 16S <i>rrs</i>, internal transcribed spacer and 23S <i>rrl</i> elements, as well as a single-copy core-gene (<i>hsp65</i>, <i>rpoB+C</i>, <i>secA</i> and <i>tuf</i>) analysis of these freeze-dried mycobacteria, clinically isolated from gastric lavage samples between 1948 and 1955, showed to be associated with type strains grouping within the Terra and Fortuitum-Vaccae clade. Phenotypic characteristics, biochemical properties and fatty acid and mycolic acid profiles supported the classification as novel strains. A genomic comparison to the closest related type strain was done by calculating average nucleotide identity and <i>in silico</i> DNA:DNA hybridization values, which showed 87.9% and 33.0% for Mu0050, 85.2% and 27.4% for Mu0053, 85.3% and 27.6% for Mu0083 and 93.3% and 50.1% for Mu0102, respectively. The names proposed for the new species are <i>Mycobacterium wendilense</i> sp. nov. (Mu0050<sup>T</sup>=ITM 501390<sup>T</sup>=CCUG 77525<sup>T</sup>), <i>Mycobacterium burgundiense</i> sp. nov. (Mu0053<sup>T</sup>=ITM 501391<sup>T</sup>=CCUG 77526<sup>T</sup>), <i>Mycobacterium kokjensenii</i> sp. nov. (Mu0083<sup>T</sup>=ITM 501392<sup>T</sup>=CCUG 77527<sup>T</sup>) and <i>Mycobacterium holstebronense</i> sp. nov. (Mu0102<sup>T</sup>=ITM 501393<sup>T</sup>=CCUG 77528<sup>T</sup>).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11706282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142948902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of Starmerella aleppica f.a., sp. nov. and large indels in the rRNA cistron that split the Starmerella genus. 星菜属分离的rRNA顺子中大索引的鉴定。
IF 2 3区 生物学 Q4 MICROBIOLOGY Pub Date : 2025-01-01 DOI: 10.1099/ijsem.0.006629
Matthias Sipiczki, Abhishek Baghela

Yeast strains representing a novel asexual ascomycetous species were isolated from seven Malva sylvestris flowers. Sequencing of the chromosomal regions coding for the D1/D2 domains of the large subunit ribosomal RNA, the ITS1-5.8S-ITS2 segments and parts of the gene coding for the small subunit ribosomal RNA showed that the isolates were conspecific. Comparative analysis of these sequences and the corresponding sequences of the type strains of ascomycetous yeasts revealed that the strains represent a hitherto undescribed species belonging to the sensu stricto subclade of the genus Starmerella. The new species is osmotolerant and can develop invasive pseudohyphae, but does not form spores. For the new species, the name Starmerella aleppica f.a. (forma asexualis) is proposed. The holotype, preserved in a metabolically inactive state, is CBS 12960T (extype cultures: 2-1361 and CCY 90-2-1, NCAIM Y.02123). The GenBank accession numbers of barcode sequences are JX515983 (D1/D2 domain), JX515985 (ITS1-5.8S-ITS2 and partial 18S rRNA gene), PQ613837 (TEF1 partial sequence) and PQ613838 (RPB2 partial sequence). MycoBank: MB855459. The analysis of the D1/D2 and internal transcribed spacer (ITS) sequences of the type strains of species of the genus identified multiple multinucleotide indels that can be used as taxonomic markers (InDel markers). The indel patterns of the subclades are very different and homogeneous within the subclades. This result reinforces the idea raised, but also refuted, in previous studies that the Starmerella subclades may represent different genera.

摘要从7株Malva sylvestris花中分离到一种新的无性子囊菌酵母菌株。编码大亚基核糖体RNA D1/D2结构域的染色体区域、编码小亚基核糖体RNA的ITS1-5.8S-ITS2片段和部分基因的测序结果表明,分离株具有同源性。将这些序列与子囊酵母类型菌株的相应序列进行比较分析表明,这些菌株属于Starmerella属严格意义亚枝的一个迄今未被描述的种。新种具有渗透性,可产生侵入性假菌丝,但不形成孢子。对于新种,建议将其命名为Starmerella aleppica f.a (forma asexual alis)。在代谢无活性状态下保存的全型为CBS 12960T(外型培养:2-1361和CCY 90-2-1, NCAIM Y.02123)。条形码序列的GenBank登录号为JX515983 (D1/D2结构域)、JX515985 (ITS1-5.8S-ITS2和部分18S rRNA基因)、PQ613837 (TEF1部分序列)和PQ613838 (RPB2部分序列)。MycoBank: MB855459。通过对该属种属型菌株的D1/D2和ITS序列分析,鉴定出多个可作为分类标记的多核苷酸序列(InDel标记)。亚支系的indel模式在亚支系内差别很大,而且是均匀的。这一结果加强了先前研究中提出的观点,但也驳斥了这一观点,即Starmerella亚分支可能代表不同的属。
{"title":"Identification of <i>Starmerella aleppica</i> f.a., sp. nov. and large indels in the rRNA cistron that split the <i>Starmerella</i> genus.","authors":"Matthias Sipiczki, Abhishek Baghela","doi":"10.1099/ijsem.0.006629","DOIUrl":"10.1099/ijsem.0.006629","url":null,"abstract":"<p><p>Yeast strains representing a novel asexual ascomycetous species were isolated from seven <i>Malva sylvestris</i> flowers. Sequencing of the chromosomal regions coding for the D1/D2 domains of the large subunit ribosomal RNA, the ITS1-5.8S-ITS2 segments and parts of the gene coding for the small subunit ribosomal RNA showed that the isolates were conspecific. Comparative analysis of these sequences and the corresponding sequences of the type strains of ascomycetous yeasts revealed that the strains represent a hitherto undescribed species belonging to the <i>sensu stricto</i> subclade of the genus <i>Starmerella</i>. The new species is osmotolerant and can develop invasive pseudohyphae, but does not form spores. For the new species, the name <i>Starmerella aleppica</i> f.a. (forma asexualis) is proposed. The holotype, preserved in a metabolically inactive state, is CBS 12960<sup>T</sup> (extype cultures: 2-1361 and CCY 90-2-1, NCAIM Y.02123). The GenBank accession numbers of barcode sequences are JX515983 (D1/D2 domain), JX515985 (ITS1-5.8S-ITS2 and partial 18S rRNA gene), PQ613837 (<i>TEF1</i> partial sequence) and PQ613838 (<i>RPB2</i> partial sequence). MycoBank: MB855459. The analysis of the D1/D2 and internal transcribed spacer (ITS) sequences of the type strains of species of the genus identified multiple multinucleotide indels that can be used as taxonomic markers (InDel markers). The indel patterns of the subclades are very different and homogeneous within the subclades. This result reinforces the idea raised, but also refuted, in previous studies that the <i>Starmerella</i> subclades may represent different genera.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142948905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Description of Veillonella absiana sp. nov., isolated from pig faeces. 猪粪便中分离的苦纹细孔菌的描述。
IF 2 3区 生物学 Q4 MICROBIOLOGY Pub Date : 2025-01-01 DOI: 10.1099/ijsem.0.006643
Lu Bai, Jayoung Paek, Yeseul Shin, Hongik Kim, Si Hyun Kim, Jeong Hwan Shin, Joong-Ki Kook, Young-Hyo Chang

Two Gram-stain-negative cocci anaerobes were isolated from pig faeces and designated as strains YH-vei2232T and YH-vei2233. Phylogenetic analysis using 16S rRNA gene sequences revealed that the isolates were most closely related to Veillonella rogosae KCTC 5967T, with 97.0% similarity. Analysis of housekeeping gene sequences (rpoB) revealed the strain formed a sub-cluster within the genus Veillonella. The average nucleotide identities between the two isolates and the most closely related strains within genus Veillonella were <75.0%. The major fatty acids were Summed Feature 8 and C16 : 1  ω9c. The cell wall peptidoglycan contained meso-diaminopimelic acid. The major metabolic end products of isolates were propionic and acetic acids. The genomic DNA G+C contents of both strains were 40.1 mol%. The chemotaxonomic, phenotypic and phylogenetic properties of YH-vei2232T (=KCTC 25748T=NBRC 116427T) and YH-vei2233 (=KCTC 25749=NBRC 116428) suggest that they represent a novel species of the genus Veillonella, for which the name Veillonella absiana sp. nov. is proposed.

从猪粪便中分离到2株革兰氏阴性厌氧菌,分别命名为YH-vei2232T和YH-vei2233。16S rRNA基因序列的系统发育分析表明,分离株与rogosae Veillonella KCTC 5967T亲缘关系最近,相似度为97.0%。内务基因序列(rpoB)分析显示该菌株形成了细孔菌属的一个亚群。两株分离株与细孔菌属内亲缘关系最密切的菌株的平均核苷酸同源度为16.1 ω9c。细胞壁肽聚糖含有中二氨基亚苯甲酸。菌株的主要代谢终产物为丙酸和乙酸。两菌株基因组DNA G+C含量均为40.1% mol%。YH-vei2232T (=KCTC 25748T=NBRC 116427T)和YH-vei2233 (=KCTC 25749=NBRC 116428)的化学分类、表型和系统发育特征表明,它们代表了一种新种,并建议将其命名为absiana sp. nov.。
{"title":"Description of <i>Veillonella absiana</i> sp. nov., isolated from pig faeces.","authors":"Lu Bai, Jayoung Paek, Yeseul Shin, Hongik Kim, Si Hyun Kim, Jeong Hwan Shin, Joong-Ki Kook, Young-Hyo Chang","doi":"10.1099/ijsem.0.006643","DOIUrl":"https://doi.org/10.1099/ijsem.0.006643","url":null,"abstract":"<p><p>Two Gram-stain-negative cocci anaerobes were isolated from pig faeces and designated as strains YH-vei2232<sup>T</sup> and YH-vei2233. Phylogenetic analysis using 16S rRNA gene sequences revealed that the isolates were most closely related to <i>Veillonella rogosae</i> KCTC 5967<sup>T</sup>, with 97.0% similarity. Analysis of housekeeping gene sequences (<i>rpoB</i>) revealed the strain formed a sub-cluster within the genus <i>Veillonella</i>. The average nucleotide identities between the two isolates and the most closely related strains within genus <i>Veillonella</i> were <75.0%. The major fatty acids were Summed Feature 8 and C<sub>16 : 1</sub> <i> ω9</i>c. The cell wall peptidoglycan contained <i>meso</i>-diaminopimelic acid. The major metabolic end products of isolates were propionic and acetic acids. The genomic DNA G+C contents of both strains were 40.1 mol%. The chemotaxonomic, phenotypic and phylogenetic properties of YH-vei2232<sup>T</sup> (=KCTC 25748<sup>T</sup>=NBRC 116427<sup>T</sup>) and YH-vei2233 (=KCTC 25749=NBRC 116428) suggest that they represent a novel species of the genus <i>Veillonella</i>, for which the name <i>Veillonella absiana</i> sp. nov. is proposed.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142983547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Tenacibaculum platacis sp. nov., Tenacibaculum vairaonense sp. nov. and Tenacibaculum polynesiense sp. nov. isolated from batfish (Platax orbicularis) in Tahiti Island, French Polynesia. 法属波利尼西亚塔希提岛蝙蝠鱼(Platax orbicularis)中分离的platacbaculum platacbaculum sp. nov., vairaonense sp. 11 .和polynesiense sp. 11 .。
IF 2 3区 生物学 Q4 MICROBIOLOGY Pub Date : 2025-01-01 DOI: 10.1099/ijsem.0.006605
Pierre Lopez, Benjamin Fradet, Lucie Coffion, Jean-François Bernardet, Denis Saulnier, Eric Duchaud

Ten novel Gram-negative, aerobic, non-sporulating, yellow-pigmented rod-shaped bacterial strains motile by gliding were isolated from marine organisms/environments in French Polynesia. Three of them designated as 190524A05cT, 190524A02bT and 190130A14aT were retrieved from orbicular batfish (Platax orbicularis) mucus. Online database comparisons using 16S rRNA amplicons resulted in over 95% similarity to the genus Tenacibaculum. Phylogenetic analyses based on 679 concatenated core protein sequences revealed that strains 190524A05cT, 190524A02bT and 190130A14aT showed the highest similarity to Tenacibaculum skagerrakense DSM 14836T, Tenacibaculum xiamenense LMG 27422T and Tenacibaculum holothuriorum S2-2T, respectively. Digital DNA-DNA hybridization and average nt identity values between strains 190524A05cT, 190524A02bT and 190130A14aT and other type strains were less than 76.25 and 24.1%, respectively. The DNA G+C content was 31.48, 30.66 and 31.98 mol% for strains 190524A05cT, 190524A02bT and 190130A14aT, respectively. Menaquinone-6 was detected as the major isoprenoid quinone in these three strains. The major polar lipids (phosphatidylethanolamine and aminophospholipid) were similar to the chemotaxonomic profile of other species of the genus Tenacibaculum. Strain 190524A05cT contained summed feature 3 (comprising C16:1 ω7c and/or iso-C15:0 2-OH), iso-C15:1 G, iso-C15:0 and iso-C17:0 3-OH as the major cellular fatty acids. Strain 190524A02bT contained summed feature 3 (comprising C16:1 ω7c and/or iso-C15:0 2-OH), iso-C15:0, iso-C15:1 G and iso-C17:0 3-OH as the major cellular fatty acids. Strain 190130A14aT contained iso-C15:1 G, summed feature 3 (comprising C16:1 ω7c and/or iso-C15:0 2-OH), iso-C15:0 and iso-C17:0 3-OH as the major cellular fatty acids. Based on the phenotypic and molecular features, these three strains represent novel species of the genus Tenacibaculum for which the names Tenacibaculum platacis sp. nov., with 190524A05cT (= CIP 112470T = DSM 118113T) as the type strain; Tenacibaculum vairaonense sp. nov., with 190524A02bT (= CIP 112469T = DSM 118112T) as the type strain; and Tenacibaculum polynesiense sp. nov., with 190130A14aT (= CIP 112468T = DSM 118111T) as the type strain, are proposed.

从法属波利尼西亚的海洋生物/环境中分离到10株新的革兰氏阴性、需氧、无孢子、黄色色素杆状细菌菌株。其中3个从圆形蝙蝠鱼(Platax orbicularis)粘液中提取,编号为190524A05cT、190524A02bT和190130A14aT。使用16S rRNA扩增子进行在线数据库比较,结果与Tenacibaculum属的相似性超过95%。基于679条核心蛋白序列的系统发育分析显示,菌株190524A05cT、190524A02bT和190130A14aT与skagerrakense Tenacibaculum DSM 14836T、厦门Tenacibaculum ximenense LMG 27422T和holothuriorum S2-2T的相似性最高。菌株190524A05cT、190524A02bT和190130A14aT与其他型菌株的数字DNA-DNA杂交和平均nt同源值分别小于76.25%和24.1%。菌株190524A05cT、190524A02bT和190130A14aT的DNA G+C含量分别为31.48、30.66和31.98 mol%。甲基萘醌-6是这3株菌株中主要的类异戊二烯醌。主要极性脂质(磷脂酰乙醇胺和氨基磷脂)与Tenacibaculum属其他物种的化学分类特征相似。菌株190524A05cT的主要细胞脂肪酸为总特征3(含C16:1 ω7c和/或iso-C15:0 2-OH)、iso-C15:1 G、iso-C15:0和iso-C17:0 3- oh。菌株190524A02bT的主要细胞脂肪酸为总特征3(含C16:1 ω7c和/或iso-C15:0 2-OH)、iso-C15:0、iso-C15:1 G和iso-C17:0 3- oh。菌株190130A14aT含有iso-C15:1 G,特征3(包括C16:1 ω7c和/或iso-C15:0 2-OH), iso-C15:0和iso-C17:0 3- oh为主要细胞脂肪酸。基于表型和分子特征,这3株菌株代表了Tenacibaculum属的新种,命名为Tenacibaculum platacis sp. nov.,类型菌株为190524A05cT (= CIP 112470T = DSM 118113T);vairaonense Tenacibaculum sp. nov.,以190524A02bT (= CIP 112469T = DSM 118112T)为型菌株;提出了以190130A14aT (= CIP 112468T = DSM 118111T)为类型菌株的波利尼西亚腱菌sp. nov.。
{"title":"<i>Tenacibaculum platacis</i> sp. nov., <i>Tenacibaculum vairaonense</i> sp. nov. and <i>Tenacibaculum polynesiense</i> sp. nov. isolated from batfish (<i>Platax orbicularis</i>) in Tahiti Island, French Polynesia.","authors":"Pierre Lopez, Benjamin Fradet, Lucie Coffion, Jean-François Bernardet, Denis Saulnier, Eric Duchaud","doi":"10.1099/ijsem.0.006605","DOIUrl":"https://doi.org/10.1099/ijsem.0.006605","url":null,"abstract":"<p><p>Ten novel Gram-negative, aerobic, non-sporulating, yellow-pigmented rod-shaped bacterial strains motile by gliding were isolated from marine organisms/environments in French Polynesia. Three of them designated as 190524A05c<sup>T</sup>, 190524A02b<sup>T</sup> and 190130A14a<sup>T</sup> were retrieved from orbicular batfish (<i>Platax orbicularis</i>) mucus. Online database comparisons using 16S rRNA amplicons resulted in over 95% similarity to the genus <i>Tenacibaculum</i>. Phylogenetic analyses based on 679 concatenated core protein sequences revealed that strains 190524A05c<sup>T</sup>, 190524A02b<sup>T</sup> and 190130A14a<sup>T</sup> showed the highest similarity to <i>Tenacibaculum skagerrakense</i> DSM 14836<sup>T</sup>, <i>Tenacibaculum xiamenense</i> LMG 27422<sup>T</sup> and <i>Tenacibaculum holothuriorum</i> S2-2<sup>T</sup>, respectively. Digital DNA-DNA hybridization and average nt identity values between strains 190524A05c<sup>T</sup>, 190524A02b<sup>T</sup> and 190130A14a<sup>T</sup> and other type strains were less than 76.25 and 24.1%, respectively. The DNA G+C content was 31.48, 30.66 and 31.98 mol% for strains 190524A05c<sup>T</sup>, 190524A02b<sup>T</sup> and 190130A14a<sup>T</sup>, respectively. Menaquinone-6 was detected as the major isoprenoid quinone in these three strains. The major polar lipids (phosphatidylethanolamine and aminophospholipid) were similar to the chemotaxonomic profile of other species of the genus <i>Tenacibaculum</i>. Strain 190524A05c<sup>T</sup> contained summed feature 3 (comprising C<sub>16:1</sub> ω7c and/or iso-C<sub>15:0</sub> 2-OH), iso-C<sub>15:1</sub> G, iso-C<sub>15:0</sub> and iso-C<sub>17:0</sub> 3-OH as the major cellular fatty acids. Strain 190524A02b<sup>T</sup> contained summed feature 3 (comprising C<sub>16:1</sub> ω7c and/or iso-C<sub>15:0</sub> 2-OH), iso-C<sub>15:0</sub>, iso-C<sub>15:1</sub> G and iso-C<sub>17:0</sub> 3-OH as the major cellular fatty acids. Strain 190130A14a<sup>T</sup> contained iso-C<sub>15:1</sub> G, summed feature 3 (comprising C<sub>16:1</sub> ω7c and/or iso-C<sub>15:0</sub> 2-OH), iso-C<sub>15:0</sub> and iso-C<sub>17:0</sub> 3-OH as the major cellular fatty acids. Based on the phenotypic and molecular features, these three strains represent novel species of the genus <i>Tenacibaculum</i> for which the names <i>Tenacibaculum platacis</i> sp. nov., with 190524A05c<sup>T</sup> (= CIP 112470<sup>T</sup> = DSM 118113<sup>T</sup>) as the type strain; <i>Tenacibaculum vairaonense</i> sp. nov., with 190524A02b<sup>T</sup> (= CIP 112469<sup>T</sup> = DSM 118112<sup>T</sup>) as the type strain; and <i>Tenacibaculum polynesiense</i> sp. nov., with 190130A14a<sup>T</sup> (= CIP 112468<sup>T</sup> = DSM 118111<sup>T</sup>) as the type strain, are proposed.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142931788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Tistrella arctica sp. nov., isolated from the surface seawater of the Bering Sea and Chukchi Sea and emended description of the genus Tistrella. 从白令海和楚科奇海表面海水中分离出来的Tistrella arctic sp. nov.及对Tistrella属的订正描述。
IF 2 3区 生物学 Q4 MICROBIOLOGY Pub Date : 2025-01-01 DOI: 10.1099/ijsem.0.006627
Jinzhu Zhang, Jianning Wang, Qiliang Lai, Xiupian Liu, Guangyu Li, Panyi Pan, Ziyuan He, Zongze Shao, Chunming Dong

Two Gram-stain-negative, motile, non-spore-forming, aerobic or facultative anaerobic and short rod-shaped bacterial strains, 25B02-3T and BH-R2-4, were isolated from surface seawater collected from the Bering Sea and Chukchi Sea, respectively. The 16S rRNA gene sequences of the two strains were identical. The phylogenetic analysis of the 16S rRNA gene sequences indicated that they were related to the genus Tistrella and shared 99.6 and 98.2% sequence similarity with T. bauzanensis BZ78T and T. mobilis IAM 14872T, respectively. Both 16S rRNA gene and genome sequence-based phylogenetic analyses showed that the two strains formed a monophyletic clade within the genus Tistrella, indicating that they may represent a novel species. The digital DNA‒DNA hybridization (dDDH) values and average nucleotide identities (ANI) between the two strains were 93 and 99%, respectively, indicating that they are different strains. The dDDH and ANI values between the two strains and the type strains of the genus Tistrella were 22.4-58.3% and 81.0-95.0%, respectively. These data clearly demonstrated that the two strains represent a separate genomic species of the genus Tistrella. The principal fatty acids were Sum In Feature 8 (C18 : 1 ω7c or C18 : 1 ω6c), C19 : 0 cyclo ω8c, Sum In Feature 2 (C12 : 0 aldehyde or unknown 10.928) and C16 : 0. The predominant respiratory quinone was Q-10, with a minor Q-9. The polar lipids included phosphatidylethanolamine, aminolipids and phospholipids. The genomic DNA G+C contents of strains 25B02-3T and BH-R2-4 were 67.3 mol% and 67.4 mol%, respectively. On the basis of the polyphasic evidence presented in this study, the two strains represent a novel species within the genus Tistrella, for which the name Tistrella arctica sp. nov. is proposed. The type strain is 25B02-3T (=MCCC 1A07333T=KCTC 8340T).

从白令海和楚科奇海采集的表层海水中分别分离到革兰氏染色阴性、运动、不产孢子、好氧或兼性厌氧、短杆状的2株细菌25B02-3T和BH-R2-4。两株的16S rRNA基因序列相同。对16S rRNA基因序列的系统发育分析表明,该基因序列与Tistrella属同源,与bauzanensis BZ78T和T. mobilis IAM 14872T的序列相似性分别为99.6%和98.2%。基于16S rRNA基因和基因组序列的系统发育分析表明,这两个菌株在Tistrella属内形成了一个单系分支,表明它们可能代表一个新种。两菌株的数字DNA-DNA杂交(dDDH)值和平均核苷酸同一性(ANI)值分别为93和99%,表明它们是不同的菌株。两株与鼠霉属型株的dDDH和ANI值分别为22.4 ~ 58.3%和81.0 ~ 95.0%。这些数据清楚地表明,这两个菌株代表了一个独立的基因组物种的Tistrella属。主要脂肪酸是Sum In Feature 8 (C18: 1 ω7c或C18: 1 ω6c)、C19: 0 cyclo ω8c、Sum In Feature 2 (C12: 0醛或未知10.928)和C16: 0。呼吸醌以Q-10为主,Q-9次之。极性脂质包括磷脂酰乙醇胺、氨基脂和磷脂。菌株25B02-3T和BH-R2-4的基因组DNA G+C含量分别为67.3%和67.4%。根据本研究提供的多相证据,这两个菌株代表了Tistrella属中的一个新种,并建议将其命名为Tistrella arctica sp. 11。型应变为25B02-3T (=MCCC 1A07333T=KCTC 8340T)。
{"title":"<i>Tistrella arctica</i> sp. nov., isolated from the surface seawater of the Bering Sea and Chukchi Sea and emended description of the genus <i>Tistrella</i>.","authors":"Jinzhu Zhang, Jianning Wang, Qiliang Lai, Xiupian Liu, Guangyu Li, Panyi Pan, Ziyuan He, Zongze Shao, Chunming Dong","doi":"10.1099/ijsem.0.006627","DOIUrl":"https://doi.org/10.1099/ijsem.0.006627","url":null,"abstract":"<p><p>Two Gram-stain-negative, motile, non-spore-forming, aerobic or facultative anaerobic and short rod-shaped bacterial strains, 25B02-3<sup>T</sup> and BH-R2-4, were isolated from surface seawater collected from the Bering Sea and Chukchi Sea, respectively. The 16S rRNA gene sequences of the two strains were identical. The phylogenetic analysis of the 16S rRNA gene sequences indicated that they were related to the genus <i>Tistrella</i> and shared 99.6 and 98.2% sequence similarity with <i>T. bauzanensis</i> BZ78<sup>T</sup> and <i>T. mobilis</i> IAM 14872<sup>T</sup>, respectively. Both 16S rRNA gene and genome sequence-based phylogenetic analyses showed that the two strains formed a monophyletic clade within the genus <i>Tistrella</i>, indicating that they may represent a novel species. The digital DNA‒DNA hybridization (dDDH) values and average nucleotide identities (ANI) between the two strains were 93 and 99%, respectively, indicating that they are different strains. The dDDH and ANI values between the two strains and the type strains of the genus <i>Tistrella</i> were 22.4-58.3% and 81.0-95.0%, respectively. These data clearly demonstrated that the two strains represent a separate genomic species of the genus <i>Tistrella</i>. The principal fatty acids were Sum In Feature 8 (C<sub>18 : 1</sub> <i>ω7</i>c or C<sub>18 : 1</sub> <i>ω6</i>c), C<sub>19 : 0</sub> cyclo <i>ω</i>8<i>c</i>, Sum In Feature 2 (C<sub>12 : 0</sub> aldehyde or unknown 10.928) and C<sub>16 : 0</sub>. The predominant respiratory quinone was Q-10, with a minor Q-9. The polar lipids included phosphatidylethanolamine, aminolipids and phospholipids. The genomic DNA G+C contents of strains 25B02-3<sup>T</sup> and BH-R2-4 were 67.3 mol% and 67.4 mol%, respectively. On the basis of the polyphasic evidence presented in this study, the two strains represent a novel species within the genus <i>Tistrella</i>, for which the name <i>Tistrella arctica</i> sp. nov. is proposed. The type strain is 25B02-3<sup>T</sup> (=MCCC 1A07333<sup>T</sup>=KCTC 8340<sup>T</sup>).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142931792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Leptospira gorisiae sp. nov, L. cinconiae sp. nov, L. mgodei sp. nov, L. milleri sp. nov and L. iowaensis sp. nov: five new species isolated from water sources in the Midwestern United States. 戈尔氏钩端螺旋体、cinconiae钩端螺旋体、mgodei钩端螺旋体、milleri钩端螺旋体和爱荷华钩端螺旋体:美国中西部水源分离的五新种。
IF 2 3区 生物学 Q4 MICROBIOLOGY Pub Date : 2025-01-01 DOI: 10.1099/ijsem.0.006595
Camila Hamond, Bienvenido Tibbs-Cortes, Luis G V Fernandes, Karen LeCount, Ellie J Putz, Tammy Anderson, Patrick Camp, Tod Stuber, Jessica Hicks, Hans van der Linden, Priscyla Dos Santos Ribeiro, Darrell O Bayles, Linda K Schlater, Jarlath E Nally

Isolates of Leptospira spp. were cultured from water sources at five different sites in central Iowa in the Midwestern United States and characterized by whole-genome sequencing. Isolates were helix-shaped and motile. Genome sequence analyses determined that the isolates could be clearly distinguished from other species described in the genus Leptospira and included one species that belonged to the pathogen subclade P1, one species that belonged to the pathogen subclade P2 and three species that belonged to the saprophyte subclade S1. The names Leptospira gorisiae sp. nov. (type strain WS92.C1T=NVSL-WS92.C1T=KIT0303T), Leptospira cinconiae sp. nov. (type strain WS58.C1T=NVSL-WS58.C1T=KIT0304T), Leptospira mgodei sp. nov. (type strain WS4.C2T=NVSL.WS4.C2T=KIT0305T), Leptospira iowaensis sp. nov. (type strain WS39.C2T=NVSL-WS39.C2T=KIT0306T) and Leptospira milleri sp. nov. (type strain WS60.C2T=NVSL-WS60.C2T=KIT0307T) are proposed.

从美国中西部爱荷华州中部五个不同地点的水源中培养钩端螺旋体分离株,并对其进行全基因组测序。分离株呈螺旋状,可运动。基因组序列分析表明,分离物与其他钩端螺旋体属物种有明显的区别,包括病原亚分支P1的1种、病原亚分支P2的1种和腐生亚分支S1的3种。提出了11月哥氏钩端螺旋体(型株WS92.C1T=NVSL-WS92.C1T=KIT0303T)、11月cinconileptospirae(型株WS58.C1T=NVSL-WS58.C1T=KIT0304T)、11月mgodei钩端螺旋体(型株WS4.C2T=NVSL.WS4.C2T=KIT0305T)、11月iowaicle螺旋体(型株WS39.C2T=NVSL-WS39.C2T=KIT0306T)和11月milleri钩端螺旋体(型株WS60.C2T=NVSL-WS60.C2T=KIT0307T)的命名。
{"title":"<i>Leptospira gorisiae</i> sp. nov, <i>L</i>. <i>cinconiae</i> sp. nov, <i>L</i>. <i>mgodei</i> sp. nov, <i>L</i>. <i>milleri</i> sp. nov and <i>L</i>. <i>iowaensis</i> sp. nov: five new species isolated from water sources in the Midwestern United States.","authors":"Camila Hamond, Bienvenido Tibbs-Cortes, Luis G V Fernandes, Karen LeCount, Ellie J Putz, Tammy Anderson, Patrick Camp, Tod Stuber, Jessica Hicks, Hans van der Linden, Priscyla Dos Santos Ribeiro, Darrell O Bayles, Linda K Schlater, Jarlath E Nally","doi":"10.1099/ijsem.0.006595","DOIUrl":"https://doi.org/10.1099/ijsem.0.006595","url":null,"abstract":"<p><p>Isolates of <i>Leptospira</i> spp. were cultured from water sources at five different sites in central Iowa in the Midwestern United States and characterized by whole-genome sequencing. Isolates were helix-shaped and motile. Genome sequence analyses determined that the isolates could be clearly distinguished from other species described in the genus <i>Leptospir</i>a and included one species that belonged to the pathogen subclade P1, one species that belonged to the pathogen subclade P2 and three species that belonged to the saprophyte subclade S1. The names <i>Leptospira gorisiae</i> sp. nov. (type strain WS92.C1<sup>T</sup>=NVSL-WS92.C1<sup>T</sup>=KIT0303<sup>T</sup>), <i>Leptospira cinconiae</i> sp. nov. (type strain WS58.C1<sup>T</sup>=NVSL-WS58.C1<sup>T</sup>=KIT0304<sup>T</sup>), <i>Leptospira mgodei</i> sp. nov. (type strain WS4.C2<sup>T</sup>=NVSL.WS4.C2<sup>T</sup>=KIT0305<sup>T</sup>), <i>Leptospira iowaensis</i> sp. nov. (type strain WS39.C2<sup>T</sup>=NVSL-WS39.C2<sup>T</sup>=KIT0306<sup>T</sup>) and <i>Leptospira milleri</i> sp. nov. (type strain WS60.C2<sup>T</sup>=NVSL-WS60.C2<sup>T</sup>=KIT0307<sup>T</sup>) are proposed.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11706286/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142948709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
International Committee on Systematics of Prokaryotes Subcommittee on the taxonomy of Chlamydiae: minutes of the closed meeting, Zoom meeting, 24 September 2024.
IF 2 3区 生物学 Q4 MICROBIOLOGY Pub Date : 2025-01-01 DOI: 10.1099/ijsem.0.006670
Nicole Borel, Gilbert Greub, For The Icsp Subcommittee On The Taxonomy Of

Subcommittee on the taxonomy of Chlamydiae: minutes of the closed meeting, Zoom meeting, 24 September 2024.

{"title":"International Committee on Systematics of Prokaryotes Subcommittee on the taxonomy of <i>Chlamydiae</i>: minutes of the closed meeting, Zoom meeting, 24 September 2024.","authors":"Nicole Borel, Gilbert Greub, For The Icsp Subcommittee On The Taxonomy Of","doi":"10.1099/ijsem.0.006670","DOIUrl":"https://doi.org/10.1099/ijsem.0.006670","url":null,"abstract":"<p><p>Subcommittee on the taxonomy of <i>Chlamydiae</i>: minutes of the closed meeting, Zoom meeting, 24 September 2024.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
International journal of systematic and evolutionary microbiology
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