Haelim Son, Wonsik Mun, Robert J Mitchell, Gyu-Sung Cho, Ja-Young Moon, Kyoung Lee
Five pink-pigmented bacterial strains, isolated from human skin and classified within the genus Roseomonas, were examined. Among them, four were identified as Roseomonas mucosa, while strain OT10T was deemed to be a potential novel species. Strain OT10T exhibited characteristics, such as Gram-stain-negative, oxidase positive, motile, strictly aerobic and rod shaped. The cells had multiple flagella at one end, arranged in a lophotrichous pattern. The predominant cellular fatty acids in OT10T were C18:1 ω7c/C18:1 ω6c and C18:1 2OH; ubiquinone (Q)-10 was identified as the sole quinone. Major polar lipids included phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine and two aminolipids. The G+C content of the genome was determined to be 72.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarities revealed that strain OT10T is closely related to Roseomonas gilardii subsp. gilardii ATCC 49956T (97.7%), Roseomonas gilardii subsp. rosea ATCC BAA-691T (97.7%) and R. mucosa ATCC BAA-692T (97.5%). For the comparative genomic analyses, whole-genome sequencing was also conducted for strain OT10T. Considering the chemotaxonomic, genotypic and phenotypic features, as well as the low average nucleotide identity and digital DNA-DNA hybridization values compared to its closest phylogenomic neighbours, OT10T is proposed to be a novel species named Roseomonas cutis sp. nov., with OT10T designated as the type strain (=KCTC 92087T =JCM 34968T).
{"title":"<i>Roseomonas cutis</i> sp. nov., isolated from human epidermis.","authors":"Haelim Son, Wonsik Mun, Robert J Mitchell, Gyu-Sung Cho, Ja-Young Moon, Kyoung Lee","doi":"10.1099/ijsem.0.006617","DOIUrl":"https://doi.org/10.1099/ijsem.0.006617","url":null,"abstract":"<p><p>Five pink-pigmented bacterial strains, isolated from human skin and classified within the genus <i>Roseomonas</i>, were examined. Among them, four were identified as <i>Roseomonas mucosa</i>, while strain OT10<sup>T</sup> was deemed to be a potential novel species. Strain OT10<sup>T</sup> exhibited characteristics, such as Gram-stain-negative, oxidase positive, motile, strictly aerobic and rod shaped. The cells had multiple flagella at one end, arranged in a lophotrichous pattern. The predominant cellular fatty acids in OT10<sup>T</sup> were C<sub>18:1</sub> ω7c/C<sub>18:1</sub> ω6c and C<sub>18:1</sub> 2OH; ubiquinone (Q)-10 was identified as the sole quinone. Major polar lipids included phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine and two aminolipids. The G+C content of the genome was determined to be 72.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarities revealed that strain OT10<sup>T</sup> is closely related to <i>Roseomonas gilardii</i> subsp. <i>gilardii</i> ATCC 49956<sup>T</sup> (97.7%), <i>Roseomonas gilardii</i> subsp. <i>rosea</i> ATCC BAA-691<sup>T</sup> (97.7%) and <i>R. mucosa</i> ATCC BAA-692<sup>T</sup> (97.5%). For the comparative genomic analyses, whole-genome sequencing was also conducted for strain OT10<sup>T</sup>. Considering the chemotaxonomic, genotypic and phenotypic features, as well as the low average nucleotide identity and digital DNA-DNA hybridization values compared to its closest phylogenomic neighbours, OT10<sup>T</sup> is proposed to be a novel species named <i>Roseomonas cutis</i> sp. nov., with OT10<sup>T</sup> designated as the type strain (=KCTC 92087<sup>T</sup> =JCM 34968<sup>T</sup>).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142948770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Following a proposal for further integration of Candidatus names into the International Code of Nomenclature of Prokaryotes, I here report the outcome of the ballot on this proposal by the members of the International Committee on Systematics of Prokaryotes.
{"title":"Addition of Section 10, Rules 66-73 for further integration of <i>Candidatus</i> names into the International Code of Nomenclature of Prokaryotes.","authors":"Aharon Oren","doi":"10.1099/ijsem.0.006638","DOIUrl":"https://doi.org/10.1099/ijsem.0.006638","url":null,"abstract":"<p><p>Following a proposal for further integration of <i>Candidatus</i> names into the International Code of Nomenclature of Prokaryotes, I here report the outcome of the ballot on this proposal by the members of the International Committee on Systematics of Prokaryotes.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142948825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Two strains of Stachybotrys, identified based on morphology and phylogenetic analysis, were isolated from rocky desertification soils in Yunnan province. Phylogenetic analyses inferred from three loci (the internal transcribed spacer of the nuclear ribosomal RNA gene, β-tubulin and RNA polymerase II second-largest subunit) showed that the two strains formed a single clade and were introduced as a new species of Stachybotrys, S. qujingensis. S. qujingensis is characterized by having ampulliform or broadly fusiform conidiogenous cells and dark olivaceous-green, oblong-ellipsoidal conidia. Phylogenetically, S. qujingensis is most closely related to S. musae, but it distinguishes the latter by longer and narrower conidia. Descriptions, illustrations and phylogenetic placement of S. qujingensis were provided.
{"title":"<i>Stachybotrys qujingensis</i> sp. nov., a new hyphomycete from desertified rocky soil in southwest China.","authors":"Xing-Wen Dai, Zhi-Yu Ren, Xue-Rong Pan, Ze-Fen Yu","doi":"10.1099/ijsem.0.006664","DOIUrl":"https://doi.org/10.1099/ijsem.0.006664","url":null,"abstract":"<p><p>Two strains of <i>Stachybotrys</i>, identified based on morphology and phylogenetic analysis, were isolated from rocky desertification soils in Yunnan province. Phylogenetic analyses inferred from three loci (the internal transcribed spacer of the nuclear ribosomal RNA gene, β-tubulin and RNA polymerase II second-largest subunit) showed that the two strains formed a single clade and were introduced as a new species of <i>Stachybotrys</i>, <i>S. qujingensis. S. qujingensis</i> is characterized by having ampulliform or broadly fusiform conidiogenous cells and dark olivaceous-green, oblong-ellipsoidal conidia. Phylogenetically, <i>S. qujingensis</i> is most closely related to <i>S. musae</i>, but it distinguishes the latter by longer and narrower conidia. Descriptions, illustrations and phylogenetic placement of <i>S. qujingensis</i> were provided.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tracy L Nicholson, Keira L Stuart, Darrell O Bayles
Three novel strains within the genus Streptococcus (29887T, 29892T and 29896T) were isolated from healthy pigs during routine veterinary physical exams. All three strains were non-motile and non-spore-forming Gram-positive cocci. The complete genome of each strain was attained, and phylogenetic analyses were performed. Comparison of the genomes of 29887T, 29892T and 29896T to the genomes of other Streptococcus strains revealed digital DNA-DNA hybridization (dDDH) values between 21.2% and 53.9% and average nucleotide identity (ANI) values between 70.00% and 94.44%. Phylogenetic analyses suggested that each strain, 29896T (S. suivaginalis sp. nov.), 29887T (S. iners sp. nov.) and 29892T (S. iners subsp. hyiners subsp. nov.), may represent a novel species within the genus Streptococcus, while ANI analysis indicated that strains 29896T (S. suivaginalis sp. nov.) and 29887T (S. iners sp. nov.) represent novel species within the genus Streptococcus, and 29892T (S. iners subsp. hyiners subsp. nov.) represents a novel subspecies of 29887T (S. iners sp. nov.). Based upon the combined data presented in this study, two novel species, Streptococcus suivaginalis sp. nov. (type strain, 29896T=NRRL B-65677T=NCTC 14941T) and Streptococcus iners sp. nov. (type strain, 29887T=NRRL B-65675T=NCTC 14939T) are proposed, and one novel subspecies, Streptococcus iners subsp. hyiners subsp. nov. (type strain, 29892T=NRRL B-65676T=NCTC 14940T) is proposed.
{"title":"<i>Streptococcus suivaginalis</i> sp. nov., <i>Streptococcus iners</i> sp. nov. and <i>Streptococcus iners</i> subsp. <i>hyiners</i> subsp. nov. isolated from pigs.","authors":"Tracy L Nicholson, Keira L Stuart, Darrell O Bayles","doi":"10.1099/ijsem.0.006631","DOIUrl":"10.1099/ijsem.0.006631","url":null,"abstract":"<p><p>Three novel strains within the genus <i>Streptococcus</i> (29887<sup>T</sup>, 29892<sup>T</sup> and 29896<sup>T</sup>) were isolated from healthy pigs during routine veterinary physical exams. All three strains were non-motile and non-spore-forming Gram-positive cocci. The complete genome of each strain was attained, and phylogenetic analyses were performed. Comparison of the genomes of 29887<sup>T</sup>, 29892<sup>T</sup> and 29896<sup>T</sup> to the genomes of other <i>Streptococcus</i> strains revealed digital DNA-DNA hybridization (dDDH) values between 21.2% and 53.9% and average nucleotide identity (ANI) values between 70.00% and 94.44%. Phylogenetic analyses suggested that each strain, 29896<sup>T</sup> (<i>S. suivaginalis</i> sp. nov.), 29887<sup>T</sup> (<i>S. iners</i> sp. nov.) and 29892<sup>T</sup> (<i>S. iners</i> subsp. <i>hyiners</i> subsp. nov.), may represent a novel species within the genus <i>Streptococcus</i>, while ANI analysis indicated that strains 29896<sup>T</sup> (<i>S. suivaginalis</i> sp. nov.) and 29887<sup>T</sup> (<i>S. iners</i> sp. nov.) represent novel species within the genus <i>Streptococcus,</i> and 29892<sup>T</sup> (<i>S. iners</i> subsp. <i>hyiners</i> subsp. nov.) represents a novel subspecies of 29887<sup>T</sup> (<i>S. iners</i> sp. nov.). Based upon the combined data presented in this study, two novel species, <i>Streptococcus suivaginalis</i> sp. nov. (type strain, 29896<sup>T</sup>=NRRL B-65677<sup>T</sup>=NCTC 14941<sup>T</sup>) and <i>Streptococcus iners</i> sp. nov. (type strain, 29887<sup>T</sup>=NRRL B-65675<sup>T</sup>=NCTC 14939<sup>T</sup>) are proposed, and one novel subspecies, <i>Streptococcus iners</i> subsp. <i>hyiners</i> subsp. nov. (type strain, 29892<sup>T</sup>=NRRL B-65676<sup>T</sup>=NCTC 14940<sup>T</sup>) is proposed.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11753468/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143004727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Two Gram-stain-negative, curved-rod-shaped, non-motile and aerobic bacteria W6T and I13T were isolated from marine sediment samples collected from Meishan Island located in the East China Sea. Catalase and oxidase activities and hydrolysis of Tween 40, 60 and 80 were positive for both strains, while nitrate reduction, indole production, methyl red reaction and H2S production were negative. Phylogenetic analyses based on 16S rRNA and genome sequences revealed that strains W6T and I13T formed distinct phylogenetic lineages within the genera Ascidiimonas and Leptobacterium, respectively. Strain W6T showed the closest relatedness to Ascidiimonas aurantiaca N5DA8-2CT with 93.9% 16S rRNA gene sequence similarity, 70.7% average nucleotide identity (ANI), 71.0% average amino acid identity (AAI) and 16.4% digital DNA-DNA hybridization (dDDH) values, while strain I13T was most closely related to Leptobacterium flavescens YM3-301T with 92.1% 16S rRNA gene sequence similarity, 70.5% ANI, 72.1% AAI and 17.2% dDDH values. The two novel strains shared 92.0% 16S rRNA gene sequence similarity to each other and were identified as two distinct species based on 70.7% ANI, 70.4% AAI and 17.1% dDDH values calculated using whole-genome sequences. The genomes of strains W6T and I13T were 4.59 Mbp with a G+C content of 34.5 mol% and 2.38 Mbp with a G+C content of 36.2 mol%, respectively. The only respiratory quinone was menaquinone-6, the major polar lipid was phosphatidylethanolamine and the major cellular fatty acids were iso-C15 : 0, iso-C15 : 1 G and iso-C17 : 0 3-OH. Based on phenotypic, chemotaxonomic and genotypic data, strains W6T and I13T are considered to represent two novel species in the genera Ascidiimonas and Leptobacterium, respectively, in the family Flavobacteriaceae, for which the names Ascidiimonas meishanensis sp. nov. and Leptobacterium meishanense sp. nov. are proposed. The type strains are W6T (=KCTC 102201T=MCCC 1K08928T) and I13T (=KCTC 102202T=MCCC 1K08929T), respectively.
{"title":"<i>Ascidiimonas meishanensis</i> sp. nov. and <i>Leptobacterium meishanense</i> sp. nov., two bacteria isolated from marine sediment in the East China Sea.","authors":"Hao Wang, Huiting Wang, Xinyu Liu, Dawoon Jung, Eun-Young Seo, Shan He, Weiyan Zhang, Lijian Ding","doi":"10.1099/ijsem.0.006653","DOIUrl":"10.1099/ijsem.0.006653","url":null,"abstract":"<p><p>Two Gram-stain-negative, curved-rod-shaped, non-motile and aerobic bacteria W6<sup>T</sup> and I13<sup>T</sup> were isolated from marine sediment samples collected from Meishan Island located in the East China Sea. Catalase and oxidase activities and hydrolysis of Tween 40, 60 and 80 were positive for both strains, while nitrate reduction, indole production, methyl red reaction and H<sub>2</sub>S production were negative. Phylogenetic analyses based on 16S rRNA and genome sequences revealed that strains W6<sup>T</sup> and I13<sup>T</sup> formed distinct phylogenetic lineages within the genera <i>Ascidiimonas</i> and <i>Leptobacterium</i>, respectively. Strain W6<sup>T</sup> showed the closest relatedness to <i>Ascidiimonas aurantiaca</i> N5DA8-2C<sup>T</sup> with 93.9% 16S rRNA gene sequence similarity, 70.7% average nucleotide identity (ANI), 71.0% average amino acid identity (AAI) and 16.4% digital DNA-DNA hybridization (dDDH) values, while strain I13<sup>T</sup> was most closely related to <i>Leptobacterium flavescens</i> YM3-301<sup>T</sup> with 92.1% 16S rRNA gene sequence similarity, 70.5% ANI, 72.1% AAI and 17.2% dDDH values. The two novel strains shared 92.0% 16S rRNA gene sequence similarity to each other and were identified as two distinct species based on 70.7% ANI, 70.4% AAI and 17.1% dDDH values calculated using whole-genome sequences. The genomes of strains W6<sup>T</sup> and I13<sup>T</sup> were 4.59 Mbp with a G+C content of 34.5 mol% and 2.38 Mbp with a G+C content of 36.2 mol%, respectively. The only respiratory quinone was menaquinone-6, the major polar lipid was phosphatidylethanolamine and the major cellular fatty acids were iso-C<sub>15 : 0</sub>, iso-C<sub>15 : 1</sub> G and iso-C<sub>17 : 0</sub> 3-OH. Based on phenotypic, chemotaxonomic and genotypic data, strains W6<sup>T</sup> and I13<sup>T</sup> are considered to represent two novel species in the genera <i>Ascidiimonas</i> and <i>Leptobacterium</i>, respectively, in the family Flavobacteriaceae, for which the names <i>Ascidiimonas meishanensis</i> sp. nov. and <i>Leptobacterium meishanense</i> sp. nov. are proposed. The type strains are W6<sup>T</sup> (=KCTC 102201<sup>T</sup>=MCCC 1K08928<sup>T</sup>) and I13<sup>T</sup> (=KCTC 102202<sup>T</sup>=MCCC 1K08929<sup>T</sup>), respectively.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143052526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Gail E Gasparich, Chih-Horng Kuo, Lucia Manso-Silván, Alain Blanchard, Mitchell F Balish
{"title":"Corrigendum: International Committee on Systematics of Prokaryotes Subcommittee on the Taxonomy of Mollicutes: minutes of the closed meeting, 7 July 2024, Gran Canaria, Spain.","authors":"Gail E Gasparich, Chih-Horng Kuo, Lucia Manso-Silván, Alain Blanchard, Mitchell F Balish","doi":"10.1099/ijsem.0.006662","DOIUrl":"https://doi.org/10.1099/ijsem.0.006662","url":null,"abstract":"","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143023419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Strain NoAHT (=KACC 23135T=JCM 35999T), a novel Gram-negative, motile bacterium with a rod-shaped morphology, was isolated from the zoo animal faecal samples, specifically the long-tailed goral species Naemorhedus caudatus. The novel bacterial strain grew optimally in a nutrient broth medium under the following conditions: 1-2% (w/v) NaCl, pH 7-8 and 30 °C. The strain NoAHT exhibited high tolerance to NaCl, with the ability to tolerate up to 7% (w/v) NaCl. Based on phylogenetic analyses using 16S rRNA gene sequencing, strain NoAHT was found to have the closest relatedness to Comamonas jiangduensis YW1T (98.5%), Comamonas aquatica ATCC 11330T (97.9%), Comamonas resistens KCTC 82561T (97.9%), Comamonas fluminis CJ34T (97.7%) and Comamonas suwonensis EJ-4T (97.6%). The genome size and genomic DNA G+C content of strain NoAHT were 4.05 Mbp and 55.9 mol%, respectively. A whole-genome-level comparison of strain NoAHT with C. jiangduensis YWT, Comamonas kerstersii LMG 3475T, C. aquatica NBRC 14918T, Comamonas terrigena NBRC 12685T and C. fluminis CJ34T revealed the following orthologous average nucleotide identity values: 80.1, 79.0, 78.6, 76.3 and 75.2%, respectively. The major polar lipids of strain NoAHT were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Considering our findings in chemotaxonomic, genotypic and phenotypic characteristics, strain NoAHT is identified as a novel species within the genus Comamonas, for which the name Comamonas halotolerans sp. nov. is proposed.
{"title":"<i>Comamonas halotolerans</i> sp. nov., isolated from the faecal sample of a zoo animal, <i>Naemorhedus caudatus</i>.","authors":"Yerim Park, Bitnara Kim, Jihyeon Min, Woojun Park","doi":"10.1099/ijsem.0.006665","DOIUrl":"https://doi.org/10.1099/ijsem.0.006665","url":null,"abstract":"<p><p>Strain NoAH<sup>T</sup> (=KACC 23135<sup>T</sup>=JCM 35999<sup>T</sup>), a novel Gram-negative, motile bacterium with a rod-shaped morphology, was isolated from the zoo animal faecal samples, specifically the long-tailed goral species <i>Naemorhedus caudatus</i>. The novel bacterial strain grew optimally in a nutrient broth medium under the following conditions: 1-2% (w/v) NaCl, pH 7-8 and 30 °C. The strain NoAH<sup>T</sup> exhibited high tolerance to NaCl, with the ability to tolerate up to 7% (w/v) NaCl. Based on phylogenetic analyses using 16S rRNA gene sequencing, strain NoAH<sup>T</sup> was found to have the closest relatedness to <i>Comamonas jiangduensis</i> YW1<sup>T</sup> (98.5%), <i>Comamonas aquatica</i> ATCC 11330<sup>T</sup> (97.9%), <i>Comamonas resistens</i> KCTC 82561<sup>T</sup> (97.9%), <i>Comamonas fluminis</i> CJ34<sup>T</sup> (97.7%) and <i>Comamonas suwonensis</i> EJ-4<sup>T</sup> (97.6%). The genome size and genomic DNA G+C content of strain NoAH<sup>T</sup> were 4.05 Mbp and 55.9 mol%, respectively. A whole-genome-level comparison of strain NoAH<sup>T</sup> with <i>C. jiangduensis</i> YW<sup>T</sup>, <i>Comamonas kerstersii</i> LMG 3475<sup>T</sup>, <i>C. aquatica</i> NBRC 14918<sup>T</sup>, <i>Comamonas terrigena</i> NBRC 12685<sup>T</sup> and <i>C. fluminis</i> CJ34<sup>T</sup> revealed the following orthologous average nucleotide identity values: 80.1, 79.0, 78.6, 76.3 and 75.2%, respectively. The major polar lipids of strain NoAH<sup>T</sup> were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Considering our findings in chemotaxonomic, genotypic and phenotypic characteristics, strain NoAH<sup>T</sup> is identified as a novel species within the genus <i>Comamonas</i>, for which the name <i>Comamonas halotolerans</i> sp. nov. is proposed.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xuan Zhang, You-Jun Liao, Zi-Xuan Liu, Rui Wang, Hong-Can Liu, Yan-Yan Zheng, Yu-Guang Zhou, Ya-Jing Yu, Lu Xue, Ai-Hua Li
Five aerobic, Gram-stain-negative bacterial strains, designated as C3-2-a3T, B3-2-R+30, C3-2-a4, C3-2-M3 and C3-2-M8, were isolated from the coastal soil of LungmuCo Lake in the Tibet Autonomous Region, PR China. Phylogenetic analyses based on 16S rRNA genes and genomes indicated that these isolates belonged to the genus Luteimonas and showed a high similarity to Luteimonas suaedae LNNU 24178T (99.01%), Luteimonas endophytica RD2P54T (98.80%) and Luteimonas salinisoli SJ-92T (97.67%). The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain C3-2-a3T and related reference strains Luteimonas suaedae LNNU 24178T, Luteimonas endophytica RD2P54T and Luteimonas salinisoli SJ-92T were 91.89, 83.11 and 83.86% and 46.90, 26.90 and 28.20%, respectively. All values were below the thresholds for delineating species, supporting their classification as novel species of the genus Luteimonas. The genomic DNA G+C content of strains C3-2-a3T was 68.39%. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified phospholipids. The predominant respiratory quinone was ubiquinone-8 (Q-8), aligning with the characteristics of members of the genus Luteimonas. The major fatty acids (>10.0%) of strain C3-2-a3T were identified as iso-C11 : 0, iso-C15 : 0, iso-C16 : 0 and iso-C17 : 1 ω9c. Based on the results of phenotypic, physiological, chemotaxonomic and genotypic characterizations, we propose that the isolates represent a novel species of genus Luteimonas, for which the name Luteimonas salinilitoris sp. nov is proposed. The type strain is C3-2-a3T (=CGMCC 1.14507T=KCTC 8642T).
{"title":"<i>Luteimonas salinilitoris</i> sp. nov., isolated from the shore soil of saline lake in Tibet of China.","authors":"Xuan Zhang, You-Jun Liao, Zi-Xuan Liu, Rui Wang, Hong-Can Liu, Yan-Yan Zheng, Yu-Guang Zhou, Ya-Jing Yu, Lu Xue, Ai-Hua Li","doi":"10.1099/ijsem.0.006630","DOIUrl":"https://doi.org/10.1099/ijsem.0.006630","url":null,"abstract":"<p><p>Five aerobic, Gram-stain-negative bacterial strains, designated as C3-2-a3<sup>T</sup>, B3-2-R+30, C3-2-a4, C3-2-M3 and C3-2-M8, were isolated from the coastal soil of LungmuCo Lake in the Tibet Autonomous Region, PR China. Phylogenetic analyses based on 16S rRNA genes and genomes indicated that these isolates belonged to the genus <i>Luteimonas</i> and showed a high similarity to <i>Luteimonas suaedae</i> LNNU 24178<sup>T</sup> (99.01%), <i>Luteimonas endophytica</i> RD2P54<sup>T</sup> (98.80%) and <i>Luteimonas salinisoli</i> SJ-92<sup>T</sup> (97.67%). The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain C3-2-a3<sup>T</sup> and related reference strains <i>Luteimonas suaedae</i> LNNU 24178<sup>T</sup>, <i>Luteimonas endophytica</i> RD2P54<sup>T</sup> and <i>Luteimonas salinisoli</i> SJ-92<sup>T</sup> were 91.89, 83.11 and 83.86% and 46.90, 26.90 and 28.20%, respectively. All values were below the thresholds for delineating species, supporting their classification as novel species of the genus <i>Luteimonas</i>. The genomic DNA G+C content of strains C3-2-a3<sup>T</sup> was 68.39%. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified phospholipids. The predominant respiratory quinone was ubiquinone-8 (Q-8), aligning with the characteristics of members of the genus <i>Luteimonas</i>. The major fatty acids (>10.0%) of strain C3-2-a3<sup>T</sup> were identified as iso-C<sub>11 : 0</sub>, iso-C<sub>15 : 0</sub>, iso-C<sub>16 : 0</sub> and iso-C<sub>17 : 1</sub> <i> ω</i>9<i>c</i>. Based on the results of phenotypic, physiological, chemotaxonomic and genotypic characterizations, we propose that the isolates represent a novel species of genus <i>Luteimonas</i>, for which the name <i>Luteimonas salinilitoris s</i>p. nov is proposed. The type strain is C3-2-a3<sup>T</sup> (=CGMCC 1.14507<sup>T</sup>=KCTC 8642<sup>T</sup>).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142931785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The authors of a recent study in this journal in which the union of the genera Youhaiella and Paradevosia was proposed wrongfully indicated the correct generic name that should be used. According to Rule 24b(4) of the International Code of Nomenclature of Prokaryotes, Paradevosia Geng et al. 2015 has priority over Youhaiella Wang et al. 2015. Hence, a new combination is needed for the species that contains Youhaiella tibetensis F4T, and in this letter, Paradevosia tibetensis (Wang et al. 2015) comb. nov. is proposed by correctly adopting the earliest legitimate generic name.
最近在该杂志上发表的一项研究中,提出了Youhaiella属和Paradevosia属的合并,作者错误地指出了应该使用的正确的属名。根据《国际原核生物命名规则》第24b(4)条,Paradevosia Geng et al. 2015优先于Youhaiella Wang et al. 2015。因此,需要对含有Youhaiella tibetensis F4T的物种进行新的组合,在这封信中,Paradevosia tibetensis (Wang et al. 2015)进行了梳理。11月是通过正确采用最早的合法通用名称提出的。
{"title":"Proposal of <i>Paradevosia tibetensis</i> (Wang <i>et al</i>. 2015) comb. nov. by transferring the species <i>Youhaiella tibetensis</i> to the genus <i>Paradevosia</i>.","authors":"Meng-Syun Li","doi":"10.1099/ijsem.0.006634","DOIUrl":"https://doi.org/10.1099/ijsem.0.006634","url":null,"abstract":"<p><p>The authors of a recent study in this journal in which the union of the genera <i>Youhaiella</i> and <i>Paradevosia</i> was proposed wrongfully indicated the correct generic name that should be used. According to Rule 24b(4) of the International Code of Nomenclature of Prokaryotes, <i>Paradevosia</i> Geng <i>et al</i>. 2015 has priority over <i>Youhaiella</i> Wang <i>et al</i>. 2015. Hence, a new combination is needed for the species that contains <i>Youhaiella tibetensis</i> F4<sup>T</sup>, and in this letter, <i>Paradevosia tibetensis</i> (Wang <i>et al</i>. 2015) comb. nov. is proposed by correctly adopting the earliest legitimate generic name.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143005239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dong-Yan He, Jia-Wei Gao, Yu-Ruo Wang, Ke Cao, Yun-Fei Cao, Yang Li, Lu-Yao Wang, Xing-Cheng Wang, Lin Xu, Cong Sun
A Gram-stain-negative, aerobic and rod-shaped bacterium, designated as HZG-20T, was isolated from a tidal flat in Zhoushan, Zhejiang Province, China. The 16S rRNA sequence similarities between strain HZG-20T and Pikeienuella piscinae RR4-56T, Coraliihabitans acroporae NNCM2T, Parvibaculum indicum P31T and Zhengella mangrovi X9-2-2T were 98.9, 91.7, 91.0 and 91.0%, respectively. Colonies of strain HZG-20T were 1.4 mm in diameter, milky white, round, smooth and convex after cultivating on marine agar at 30 °C for 48 h. Cells were catalase and oxidase-negative. Growth occurred at 15-37 ℃ (optimum, 28 ℃), pH 5.0-9.0 (optimum, pH 6.0-8.0) and with 0-8% (w/v) NaCl (optimum, 1-3%). It contained Menaquinone-8 (H2) as the sole respiratory quinone, and C16:0 (11.8-13.6%), C18:1 ω9c (6.8-13.3%) and C15:0 anteiso (10.9-27.7%) as the major cellular fatty acids. The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid, one unidentified phosphoglycolipid, two unidentified glycolipids (GL1-GL2) and three unidentified lipids (L1-L3). The genome of strain HZG-20T was 3 835 886 bp in length, comprised 3746 protein-coding genes, with DNA G+C content of 67.1 mol%. The phylogenetic and phylogenomic trees indicated that strain HZG-20T formed an independent and stable clade with P. piscinae RR4-56T. However, the average nucleotide identity, digit DNA-DNA hybridization and average amino acid identity values between strain HZG-20T and P. piscinae RR4-56T, C. acroporae NNCM2T, P. indicum P31T and Z. mangrovi X9-2-2T were 81.6, 71.1, 68.7 and 69.5%; 23.0, 18.5, 17.9 and 17.5%; and 78.2, 56.8, 56.5 and 61.9%, respectively, together with distinct chemotaxonomic features, indicating strain HZG-20T should not be assigned to known genera. As a result, a novel species of a novel genus within the family Paracoccaceae, designated as Paludibacillus litoralis gen. nov., sp. nov., was proposed. The type strain is HZG-20T (MCCC 1K08468T=KCTC 82692T).
{"title":"<i>Paludibacillus litoralis</i> gen. nov., sp. nov.: a novel species of a novel genus in the family <i>Paracoccaceae</i>, isolated from the sediment of a tidal flat located in Zhoushan, China.","authors":"Dong-Yan He, Jia-Wei Gao, Yu-Ruo Wang, Ke Cao, Yun-Fei Cao, Yang Li, Lu-Yao Wang, Xing-Cheng Wang, Lin Xu, Cong Sun","doi":"10.1099/ijsem.0.006655","DOIUrl":"10.1099/ijsem.0.006655","url":null,"abstract":"<p><p>A Gram-stain-negative, aerobic and rod-shaped bacterium, designated as HZG-20<sup>T</sup>, was isolated from a tidal flat in Zhoushan, Zhejiang Province, China. The 16S rRNA sequence similarities between strain HZG-20<sup>T</sup> and <i>Pikeienuella piscinae</i> RR4-56<sup>T</sup>, <i>Coraliihabitans acroporae</i> NNCM2<sup>T</sup>, <i>Parvibaculum indicum</i> P31<sup>T</sup> and <i>Zhengella mangrovi</i> X9-2-2<sup>T</sup> were 98.9, 91.7, 91.0 and 91.0%, respectively. Colonies of strain HZG-20<sup>T</sup> were 1.4 mm in diameter, milky white, round, smooth and convex after cultivating on marine agar at 30 °C for 48 h. Cells were catalase and oxidase-negative. Growth occurred at 15-37 ℃ (optimum, 28 ℃), pH 5.0-9.0 (optimum, pH 6.0-8.0) and with 0-8% (w/v) NaCl (optimum, 1-3%). It contained Menaquinone-8 (H<sub>2</sub>) as the sole respiratory quinone, and C<sub>16:0</sub> (11.8-13.6%), C<sub>18:1</sub> <i> ω9</i>c (6.8-13.3%) and C<sub>15:0</sub> anteiso (10.9-27.7%) as the major cellular fatty acids. The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid, one unidentified phosphoglycolipid, two unidentified glycolipids (GL1-GL2) and three unidentified lipids (L1-L3). The genome of strain HZG-20<sup>T</sup> was 3 835 886 bp in length, comprised 3746 protein-coding genes, with DNA G+C content of 67.1 mol%. The phylogenetic and phylogenomic trees indicated that strain HZG-20<sup>T</sup> formed an independent and stable clade with <i>P. piscinae</i> RR4-56<sup>T</sup>. However, the average nucleotide identity, <i>digit</i> DNA-DNA hybridization and average amino acid identity values between strain HZG-20<sup>T</sup> and <i>P. piscinae</i> RR4-56<sup>T</sup>, <i>C. acroporae</i> NNCM2<sup>T</sup>, <i>P. indicum</i> P31<sup>T</sup> and <i>Z. mangrovi</i> X9-2-2<sup>T</sup> were 81.6, 71.1, 68.7 and 69.5%; 23.0, 18.5, 17.9 and 17.5%; and 78.2, 56.8, 56.5 and 61.9%, respectively, together with distinct chemotaxonomic features, indicating strain HZG-20<sup>T</sup> should not be assigned to known genera. As a result, a novel species of a novel genus within the family <i>Paracoccaceae</i>, designated as <i>Paludibacillus litoralis</i> gen. nov., sp. nov., was proposed. The type strain is HZG-20<sup>T</sup> (MCCC 1K08468<sup>T</sup>=KCTC 82692<sup>T</sup>).</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"75 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}