International journal of systematic and evolutionary microbiology最新文献

The authors of a recent study in this journal in which the union of the genera Youhaiella and Paradevosia was proposed wrongfully indicated the correct generic name that should be used. According to Rule 24b(4) of the International Code of Nomenclature of Prokaryotes, Paradevosia Geng et al. 2015 has priority over Youhaiella Wang et al. 2015. Hence, a new combination is needed for the species that contains Youhaiella tibetensis F4^T, and in this letter, Paradevosia tibetensis (Wang et al. 2015) comb. nov. is proposed by correctly adopting the earliest legitimate generic name.

A Gram-stain-negative, aerobic and rod-shaped bacterium, designated as HZG-20^T, was isolated from a tidal flat in Zhoushan, Zhejiang Province, China. The 16S rRNA sequence similarities between strain HZG-20^T and Pikeienuella piscinae RR4-56^T, Coraliihabitans acroporae NNCM2^T, Parvibaculum indicum P31^T and Zhengella mangrovi X9-2-2^T were 98.9, 91.7, 91.0 and 91.0%, respectively. Colonies of strain HZG-20^T were 1.4 mm in diameter, milky white, round, smooth and convex after cultivating on marine agar at 30 °C for 48 h. Cells were catalase and oxidase-negative. Growth occurred at 15-37 ℃ (optimum, 28 ℃), pH 5.0-9.0 (optimum, pH 6.0-8.0) and with 0-8% (w/v) NaCl (optimum, 1-3%). It contained Menaquinone-8 (H₂) as the sole respiratory quinone, and C_16:0 (11.8-13.6%), C_18:1  ω9c (6.8-13.3%) and C_15:0 anteiso (10.9-27.7%) as the major cellular fatty acids. The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid, one unidentified aminolipid, one unidentified phosphoglycolipid, two unidentified glycolipids (GL1-GL2) and three unidentified lipids (L1-L3). The genome of strain HZG-20^T was 3 835 886 bp in length, comprised 3746 protein-coding genes, with DNA G+C content of 67.1 mol%. The phylogenetic and phylogenomic trees indicated that strain HZG-20^T formed an independent and stable clade with P. piscinae RR4-56^T. However, the average nucleotide identity, digit DNA-DNA hybridization and average amino acid identity values between strain HZG-20^T and P. piscinae RR4-56^T, C. acroporae NNCM2^T, P. indicum P31^T and Z. mangrovi X9-2-2^T were 81.6, 71.1, 68.7 and 69.5%; 23.0, 18.5, 17.9 and 17.5%; and 78.2, 56.8, 56.5 and 61.9%, respectively, together with distinct chemotaxonomic features, indicating strain HZG-20^T should not be assigned to known genera. As a result, a novel species of a novel genus within the family Paracoccaceae, designated as Paludibacillus litoralis gen. nov., sp. nov., was proposed. The type strain is HZG-20^T (MCCC 1K08468^T=KCTC 82692^T).

Small, obligately anaerobic strains 13CB8C^T, 13CB11C^T, 13CB18C^T and 13GAM1G^T were isolated from a faecal sample in a patient with Parkinson's disease with a history of duodenal resection. After conducting a comprehensive polyphasic taxonomic analysis including genomic analysis, we propose the establishment of one new genus and four new species. The novel bacteria are Desulfovibrio falkowii sp. nov. (type strain JCM 36128^T = DSM 116810^T), Porphyromonas miyakawae sp. nov. (type strain JCM 36129^T = DSM 116947^T), Mediterraneibacter flintii sp. nov. (type strain JCM 36130^T = DSM 116866^T) and Owariibacterium komagatae gen. nov. sp. nov. (type strain JCM 36131^T = DSM 116982^T), respectively.

A crude oil aggregation-forming, strictly anaerobic, Gram-stain-positive, spore-forming, rod-shaped, motile and mesophilic bacterium, named strain SH18-2^T, was isolated from marine sediment near Sado Island in the Sea of Japan. The temperature, salinity and pH ranges of this strain for the growth were 15-40 °C (optimum 35 °C), 0.5-6.0% NaCl (w/v; optimum 2.0%) and pH 6.0-8.0 (optimum 7.5), respectively. A phylogenetic analysis of the 16S rRNA gene sequence showed that this strain belongs to the genus Clostridium. It was most closely related to Clostridium grantii A-1^T (97.02% homology). The draft genome size was 5616089 bp, with a G+C content of 29.7 mol%. The digital DNA-DNA hybridization values determined using the Genome-to-Genome Distance Calculator and the average nucleotide identity between this strain and C. grantii A-1^T were 20.30 and 78.24%, respectively. Whole-organism hydrolysates of strain SH18-2^T contained meso-diaminopimelic acid; the major fatty acid was C_16:0, and the polar lipid pattern consisted of diphosphatidylglycerol, phosphatidylglycerol, glycolipid and three unidentified polar lipids. From the results of genotypic, phenotypic and chemotaxonomic analyses, SH18-2^T (=NBRC 116030^T=DSM 115762^T) is the type strain and represents a novel species of the genus Clostridium, and the name Clostridium sediminicola sp. nov. is proposed.

A novel yeast species, isolated from the bark of pine trees in Gyeongju, South Korea, and designated as KCTC 37304^T (ex-type KACC 410729), is characterized by its genetic, morphological and physiological properties. Molecular phylogenetic analysis involving the D1/D2 domain of the 26S LSU rRNA gene and the internal transcribed spacer (ITS) region confirms that it belongs to the genus Chionosphaera. In comparison to Chionosphaera cuniculicola CBS:10065, the type strain of its closest relative, KCTC 37304^T exhibits 8 nucleotide substitutions (~2.07%) in the D1/D2 domain and 54 substitutions (~8.47%) in the ITS region. Morphologically, cells of KCTC 37304^T are globose to ovoid, which distinguishes them from the cylindrical cells of C. cuniculicola. Physiologically, KCTC 37304^T does not assimilate methanol or ethanol but can utilize succinate and xylitol, further differentiating it from C. cuniculicola. Based on these distinctive genetic, morphological and physiological features, we propose the new species Chionosphaera pinicorticola sp. nov., with KCTC 37304^T (ex-type strain KACC 410729) designated as the holotype.

Three aerobic, pink-pigmented, Gram-negative, motile and rod-shaped bacterial strains, designated SD21^T, SI9^T and SB2^T, were isolated from the phyllosphere of healthy litchis collected from three main producing sites of Guangdong Province, PR China. The 16S rRNA gene analysis showed that strains SD21^T and SI9^T belonged to the genus Methylobacterium (Mtb.) with the highest similarity to Mtb. komagatae DSM 19563^T (98.7%) and Mtb. phyllosphaerae CBMB27^T (99.8%), respectively, while strain SB2^T belonged to the genus Methylorubrum (Mtr.) and showed the highest similarity to Mtr. suomiense DSM 14458^T (98.6%). Phylogenomic analysis based on 92 core genes clearly showed that the most closely related type strains of SD21^T, SI9^T and SB2^T were Mtb. komagatae DSM 19563^T, Mtb. phyllostachyos ICMP 17619^T and Mtr. salsuginis CGMCC 1.6474^T, respectively. The ANI and dDDH values between the three isolates and their most closely related type strains were 85.6‒90.1% and 29.5‒40.4%, respectively, much below the threshold values for species delimitation. The isolates showed clear differences from their closely related type strains in terms of growth conditions, enzyme activities, substrates assimilation and contents of the major fatty acids. They all took summed feature 8 (C_18 : 1 ω7c and/or C_18 : 1 ω6c) as the major fatty acid, ubiquinone 10 as the predominant respiratory quinone and phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine as the major polar lipids. The phenotypic, phylogenetic and chemotaxonomic analyses with genome comparison strongly support that the isolates represent three distinct novel species within the genera of Methylobacterium and Methylorubrum, for which the names Methylobacterium litchii sp. nov., Methylobacterium guangdongense sp. nov. and Methylorubrum subtropicum sp. nov. are proposed, with SD21^T (=GDMCC 1.4327^T=KCTC 8300^T), SI9^T (=GDMCC 1.4329^T=KCTC 8298^T) and SB2^T (=GDMCC 1.4328^T=KCTC 8299^T) as the type strains, respectively.

Objective: The aim of the current clinical study was to reveal whether harvesting of a temporalis fascia graft would be associated with a higher incidence of temporomandibular joint (TMJ) internal derangement.

Methods: The study group involved 104 patients who had middle-ear operations, 67 of which involved harvesting of temporalis fascia and 37 that did not. The TMJs were clinically examined in each group.

Results: The total incidence of internal derangement of the TMJ was significantly higher in the group that had temporalis fascia harvesting (79.1%), compared to the group that did not have temporalis fascia harvesting (29.7%), (p= 0.001).

Conclusion: Harvesting of temporalis fascia probably alters mandibular kinematics and predisposes to internal derangement of the TMJs.

A novel strain of the genus Holzapfeliella, named He02^T, was isolated from flowers of Satureja montana L. in a survey for lactic acid bacteria associated with wild and cultivated plants in the metropolitan area of Valencia, Spain. Partial 16S rRNA gene sequencing revealed a similarity of 99% to Holzapfeliella floricola DSM 23037^T=Ryu1-2^T. Strain He02^T cells are Gram-stain-positive, catalase-negative non-motile rods, usually occurring in pairs. Cells show a pale yellow pigmentation when pelleted. As H. floricola, strain He02^T utilized a narrow range of carbohydrates, namely, glucose and fructose, homofermentatively. However, genome sequencing and estimation of average nucleotide identity (ANI) revealed an ANI value of 87.44 with H. floricola DSM 23037^T, the only H. floricola strain sequenced to date. A value of 30.5% for digital DNA-DNA hybridization was estimated with the Type Strain Genome Server tool when He02^T was compared with strain DSM 23037^T. These results indicate that strain He02^T constitutes a novel species, for which the name Holzapfeliella saturejae sp. nov. with He02^T (=CECT 31001^T=DSM 117324^T=CCM 9395^T) as type strain is proposed.