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Description on anatomy and histology of Echinometra mathaei (Echinoidea: Camarodonta: Echinometidae), the Persian Gulf sea urchin 波斯湾海胆,棘总目:棘总目:棘总科)解剖组织学描述
Pub Date : 2018-11-01 DOI: 10.29252/IJAAH.4.2.1
F. Piryaei, P. G. Mostafavi, D. Shahbazzadeh, K. Bagheri, Venom
Echinoids were collected at depth of 0-5 m from coastal water of Larak Island, the Persian Gulf in January 2016. Soft tissues of the animal including peristome, peritoneum, gills, podium, ampulla, axial organ, gonads and food canal were taken and fixed in %10 formalin. Five micron sections were stained by hematoxylin and eosin method and studied under light microscope. The anatomy and histology of organs including peristome, peritoneum, gills, podium, ampulla, axial organ, gonads and food canal were investigated. In oral view, peristome, mouth, teeth, gills, spines, and pedicellariae were the six major compartments. Correspondence K Pooshang Bagheri, Venom and Biotherapeutics Molecules Lab, Medical Biotechnology Dept, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran (e-mail: k_bagheri@pasteur.ac.ir). Aboral view was seen as a round shape consisted of tube feet, spines, pedicellariae, madreporite, genital plate, ocular plate and anus. In both oral and aboral view seven types of spines and five types of pedicellariae were reported. Histological evaluation showed the organization of their layers and cells. In this study some anatomical and histological features of the Persian Gulf Echinometra mathaei were characterized. The data presented in this paper could be illuminating the path for marine biologists, anatomists, and histologists to use this creature in their desired research areas, basic or industrial approaches.
2016年1月,在波斯湾Larak岛沿海水域0 ~ 5 m深度采集棘虫。取肠壁、腹膜、鳃、腹底、壶腹、轴形器官、性腺和食道等软组织,用%10福尔马林固定。采用苏木精和伊红法对5微米切片进行染色,光镜下观察。研究了胃壁、腹膜、鳃、腹腹、腹轴、性腺和食道等器官的解剖组织学。口腔观上,胃壁、口、牙、鳃、棘、马蒂为六大隔室。K Pooshang Bagheri,伊朗德黑兰巴斯德研究所生物技术研究中心,医学生物技术部,毒液和生物治疗分子实验室(e-mail: k_bagheri@pasteur.ac.ir)。产宫观呈圆形,由管足、棘、马蒂、红石、生殖板、眼板和肛门组成。在口腔和流产视图中,报告了7种类型的棘和5种类型的马蒂。组织学检查显示其层和细胞的组织。本文对波斯湾棘球蚴的一些解剖和组织学特征进行了研究。论文中提供的数据可以为海洋生物学家、解剖学家和组织学家在他们想要的研究领域、基础或工业方法中使用这种生物指明道路。
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引用次数: 0
Effects of Padina australis (Hauck) polysaccharide extract on growth, antioxidant and nonspecific immune parameters of the western white leg shrimp, Litopenaeus vannamei (Boone) 南蹄草多糖提取物对凡纳滨对虾生长、抗氧化及非特异性免疫指标的影响
Pub Date : 2018-11-01 DOI: 10.29252/IJAAH.4.2.73
P. Akbary, Z. Aminikhoei
This study aimed at determining impacts of various amounts of water-soluble polysaccharide extracts from brown algae, Padina australis (Hauck) (WPP) on actions of antioxidant enzymes, the growth, and non-specific immune response of shrimp, Litopenaeus vannamei (Boone). Triplicate groups of shrimp (1 ± 0.1g) have been given feed 4 isonitrogenous and iso-lipidic diets composing of 4 levels; that is, 0 (control), 0.5, 1.0, and 1.5 g kg of WPP extract for eight weeks. Analyses showed that significantly positive effects of diets with 1.0 g kg of WPP extract on the growth function of the studied groups. Additionally, improvements of the antioxidant enzyme activities, such as catalase, malondialdehyde, super-oxide dismutase, and glutathione peroxidase via feeding 0.5 and 1 g kg of WPP extracts. Correspondence P Akbary, Department of Marine Sciences, Chabahar Maritime University, Fisheries group, Chabahar, Iran (e-mail: paria.akbary@gmail.com). Furthermore, activity of phenoloxidase and lysozyme in the treatment fed by 0.5 and 1 g kg 1 of WPP extract have been considerably greater in comparison to the controls and 1.5 g kg treatments. Findings demonstrated that usage of 1.0 g kg of water soluble poly-saccharides extract of the P. australis are useful on growth function, antio-xidant and nonospecific immune variables of the L. vannamei.
本研究旨在研究不同量的褐藻水溶多糖提取物对凡纳滨对虾(Litopenaeus vannamei, Boone)抗氧化酶活性、生长和非特异性免疫反应的影响。3个重复组(1±0.1g)投喂4种等氮等脂饲料,组成4个水平;即0(对照)、0.5、1.0和1.5 g kg WPP提取物,持续8周。分析表明,饲粮中添加1.0 g kg WPP提取物对各试验组的生长功能有显著的正向影响。此外,饲喂0.5和1 g kg WPP提取物可提高过氧化氢酶、丙二醛、超氧化物歧化酶和谷胱甘肽过氧化物酶等抗氧化酶活性。伊朗恰巴哈尔海事大学海洋科学系渔业组P . Akbary (e-mail: paria.akbary@gmail.com)。此外,饲喂0.5和1 g kg / 1 WPP提取物的处理的酚氧化酶和溶菌酶活性显著高于对照和1.5 g kg / 1 WPP提取物的处理。结果表明,1.0 g kg水溶多糖提取物对凡纳梅的生长功能、抗氧化和非特异性免疫指标均有影响。
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引用次数: 3
The effect of replacement of cow's gelatin by Cyprinus carpio skin gelatin on the some mineral contents and color parameters of functional pastill 鲤鱼皮明胶替代牛明胶对功能性糊料中部分矿物质含量和颜色参数的影响
Pub Date : 2018-11-01 DOI: 10.29252/IJAAH.4.2.40
N. Choobkar, A. Aghajani, Abolfazl Jokar
Gelatin is a valuable protein source and a commercial hydrocolloid which is particularly applied for food, pharmaceutical, and photographic industries. Commercial gelatin is basically from pig and cow, which because of religious reasons and risk of transmission of Bovine spongiform encephalopathy (BSE) respectively, not acceptable to use. Therefore gelatin extraction from fish, and especially it s waste has considerable importance. In the present study, after the extraction of gelatin from economic fish skin of carp (Cyprinus carpio), various formulations of this gelatin with cows gelatin have been used in ratios of 0, 25, 50, 75 and 100% to pastill production. Correspondence N Choobkar, Department of Fisheries, College of Agriculture, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran (e-mail: Nchoobkar20@gmail.com). Then, the physicochemical experiments include of ash, protein, moisture, iron, phosphorus, zinc and lithium content, and color measurement were done on one day after of pastill production. The results showed that the treatment based on 75% fish gelatin had the highest moisture, protein, phosphorus, iron, ash and zinc content (p<0.05) while the highest level of potassium and lithium content was related to treatment based on 100% industrial gelatin (Cows) (p<0.05). Industrial treatment showed the highest L value (lightness) and the lowest index a (green–red) and b (blue–yellow) value (p<0.05). It can be concluded that useless waste of fish as halal gelatin can be a good substitute instead of other gelatin for children's favorite junk foods, which has higher levels of zinc and protein content with economical benefits.
明胶是一种有价值的蛋白质来源和商业水胶体,特别适用于食品,制药和摄影工业。商品明胶主要来自猪和牛,分别由于宗教原因和牛海绵状脑病(BSE)传播的风险,不能接受使用。因此,从鱼中提取明胶,特别是从鱼的废料中提取明胶具有相当重要的意义。在本研究中,从鲤鱼(Cyprinus carpio)的经济鱼皮中提取明胶后,以0,25,50,75和100%的比例将这种明胶与牛明胶混合制成各种配方。伊朗克尔曼沙伊斯兰阿扎德大学克尔曼沙分校农业学院渔业系N Choobkar (e-mail: Nchoobkar20@gmail.com)。然后,在生产后的第一天进行灰分、蛋白质、水分、铁、磷、锌、锂含量的理化实验和颜色测定。结果表明,75%鱼明胶处理的饲料水分、蛋白质、磷、铁、灰分和锌含量最高(p<0.05),而100%工业明胶(牛)处理的饲料钾和锂含量最高(p<0.05)。工业处理的L值(亮度)最高,a(绿红)和b(蓝黄)指数最低(p<0.05)。由此可见,鱼的无用废料作为清真明胶可作为儿童喜爱的垃圾食品的良好替代品,具有较高的锌和蛋白质含量,具有经济效益。
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引用次数: 1
The use of active edible coatings made from a combination of Lepidium sativum gum and Carvacrol to increase shelf life of farmed shrimp kept under refrigerator condition 用枸杞树胶和香芹酚复合制成活性食用涂层,提高养殖对虾的冷藏保存期
Pub Date : 2018-11-01 DOI: 10.29252/IJAAH.4.2.55
M. Karamkhani, S. Anvar, M. Ataee
The aim of this study was to evaluate the effect of edible coating based on Lepidium sativum seed gum containing different levels of carvacrol on microbial, physical quality and shelf life of farmed shrimp (Penaeus semisulcatus) during refrigerated storage (18 days). At first, different concentrations of carvacrol (0, 5, 10 and 15%) were added to edible coating. After that the color indexes, antioxidant and antimicrobial activities of coating samples were studied. Then the shrimp samples were covered with these coating solutions, and were evaluated the effects of them on samples quality and shelf life. The obtained results from edible coating tests showed that by increasing the carvacrol in edible coating, the antioxidant activity and L* values of samples were increased significantly, conversely, the b* value significantly was decreased (p<0.05). Microbial analysis of shrimp samples indicated that edible coating containing 10% and 15% carvacrol had significant effects on reducing microbial load (p<0.05). Correspondence S.A.A Anvar, Department of Food hygiene, Science and Research branch, Islamic Azad University, Tehran, Iran (e-mail: saaa4824@gmail.com). The smallest changes in pH and TBA amounts were seen in the coating samples enriched with 10% and 15% carvacrol. Using of coating containing different levels of carvacrol was increased L* and a* values while the b* significantly was decreased (p<0.05). The results stated that the application of edible coating enriched with carvacrol significantly improved sensory quality of samples (p<0.05). It has been confirmed that coating the shrimps with cress seed coating containing 10% and 15% carvacrol retarded the melanosis in shrimp samples. This study thus clearly indicated the effect of cress seed gum coating containing carvacrol on shrimp samples improved their good quality characteristics and the shelf life during refrigerated storage, which was supported by the results of microbiological, chemical, and sensorial properties. The shrimp treated with cress seed gum coating containing 10% carvacrol gave a good sensory quality.
本试验旨在研究含不同含量香芹酚的Lepidium sativum seed gum可食性包衣对养殖对虾(Penaeus semiulcatus)冷藏(18 d)期间微生物、物理品质和货架期的影响。首先,在食用包衣中添加不同浓度的香芹酚(0、5、10、15%)。然后对涂层样品的显色指标、抗氧化活性和抗菌活性进行了研究。然后将这些包衣液覆盖在虾样品上,并评价其对样品质量和保质期的影响。食用包衣试验结果表明,增加食用包衣中香芹酚的含量,样品的抗氧化活性和L*值显著提高,b*值显著降低(p<0.05)。对虾样品微生物分析表明,添加10%和15%香芹酚的食用包衣显著降低了虾样品的微生物负荷(p<0.05)。通信S.A.A Anvar,伊朗德黑兰伊斯兰阿扎德大学食品卫生系,科学与研究分部(e-mail: saaa4824@gmail.com)。在富含10%和15%香芹酚的涂层样品中,pH和TBA量的变化最小。添加不同水平香芹酚可显著提高肉鸡的L*和a*值,显著降低肉鸡的b*值(p<0.05)。结果表明,应用富含香芹酚的食用包衣显著提高了样品的感官品质(p<0.05)。用含有10%和15%香芹酚的水芹籽包衣对虾类黑变病有延缓作用。由此可见,芥蓝籽胶包被香芹酚后,虾类在冷藏过程中具有良好的品质特性和保质期,其微生物学、化学和感官特性的研究结果支持了这一结论。用含10%香芹酚的水芹籽胶包衣处理后,虾的感官品质较好。
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引用次数: 6
Effects of dietary Garlic extract on some blood, immunity and growth parameters of Common Carp fingerlings (Cyprinus carpio) 饲料中大蒜提取物对鲤鱼鱼苗部分血液、免疫及生长参数的影响
Pub Date : 2018-11-01 DOI: 10.29252/IJAAH.4.2.28
A. K. Pashaki, S. M. J Zorriehzahra, M. Ghasemi, M. Rohani, S. Hosseini
This research was carried out to study the effect of dietary garlic extract on some blood, immunity and growth parameters in common carp fingerlings. One hundred thirty five fish with an average weight of 15 ± 3.4 g were cultivated in 9 aquariums of 20 liters, with a density of 15 in each. Fish were fed with two diets of 1 and 5 g kg garlic extract for 8 weeks. By the end of the eighth week; growth, blood and immunity parameters were measured in fish. Mean temperature, dissolved oxygen and pH during the experiments were 17.01 ± 1.7 °C, 7.23 ± 0.41 mg L and 7.51 ± 0.81, respectively. *Correspondence S.M.J Zorriehzahra, Aquatic animal Health & Diseases Department, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran (e-mail: zorrieh@yahoo.com) Fish were fed with 5 g kg garlic extract had lower FCR (Feed Conversion Ratio) than the control group (p<0.05). Final weight, weight rate, Specific Growth Rate (SGR), and mean daily growth in treatment groups did not show a significant difference with the control group (p>0.05). The highest number of White Blood Cells (WBC), Lymphocyte, the highest amount of Lysozyme and IgM were reported in fish receiving the 5 g kg garlic extract compared to the control (p<0.05). Based on the results, garlic extract at 5 g kg supplemented diet can improve some parameters of the blood, immune and growth of Common Carp fingerlings.
本试验旨在研究饲料中添加大蒜提取物对鲤鱼鱼种血液、免疫及生长指标的影响。在9个20升的水族箱中养殖了135条鱼,平均重量为15±3.4 g,每个水族箱的密度为15条。分别饲喂1和5 g kg大蒜提取物两种饲料,为期8周。在第八周结束时;测定了鱼的生长、血液和免疫参数。实验期间的平均温度为17.01±1.7°C,溶解氧为7.23±0.41 mg L, pH为7.51±0.81。*通讯S.M.J Zorriehzahra,伊朗德黑兰农业研究教育与推广组织(AREEO)伊朗渔业科学研究所水产动物卫生与疾病部(e-mail: zorrieh@yahoo.com) 5 g kg大蒜提取物组鱼的饲料转化率(FCR)低于对照组(p0.05)。5 g kg大蒜提取物组的白细胞(WBC)、淋巴细胞、溶菌酶和IgM含量均高于对照组(p<0.05)。由此可见,饲料中添加5 g kg大蒜提取物可改善鲤鱼鱼种的血液、免疫和生长等指标。
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引用次数: 9
Antimicrobial efficiency of Allium atroviolaceum extract on Rainbow trout in different temperature and storage time 葱提取物在不同温度和贮存时间下对虹鳟鱼的抑菌效果
Pub Date : 2018-11-01 DOI: 10.29252/IJAAH.4.2.86
B. Hafeznia, S. Anvar, S. Kakookali, N. Choobkar
Foodborne diseases in nature are usually infectious or toxic, caused by bacteria, viruses, parasites or chemicals, transmitted through food or water contaminated by the human body. To control the disease, as well as to ensure that the food is safe and healthy, its risks must be checked, detected and controlled. The goals of food industry are to produce healthy and high quality products that requires no contamination with bacteria. The use of natural preservatives such as herbal extracts and essential oils is recommended. Allium atroviolaceum is a family of Alliaceae and is a perennial herb which has a high nutritional value. The role of plant extracts has been evaluated as a preservative. Correspondence S.A.A Anvar, Department of Food Hygiene, Science and Research Branch, Islamic Azad University, Tehran, Iran (e-mail: Saaa4824@gmail.com ). The total number of bacteria calculated followed by treatment of the aqueous and ethanol extractions of the A. atroviolaceum on rainbow trout fish fillet. 100 μl of aqueous and alcoholic extracts followed by ten times dilution used to evaluate the minimum inhibitory concentration, then 25 grams of rainbow trout fillet was submerged with the best concentrations of aqueous and alcoholic extracts. The results illustrated that the best aqueous and alcoholic extractions against Staphylococcus aureus was 3.125 mg ml and 6.25 mg ml and followed by treatment on fish both type of extraction showed inhibition on growth of bacteria compare to control group. So we believe that this plant has such a valuable effect to improve the quality and shelf life of fish.
自然界中的食源性疾病通常具有传染性或毒性,由细菌、病毒、寄生虫或化学物质引起,通过被人体污染的食物或水传播。为了控制疾病,确保食品安全健康,必须对其风险进行检查、检测和控制。食品工业的目标是生产健康和高质量的产品,不需要细菌污染。建议使用天然防腐剂,如草药提取物和精油。葱属葱科植物,是一种营养价值较高的多年生草本植物。植物提取物作为防腐剂的作用已得到评价。通信S.A.A Anvar,伊朗德黑兰伊斯兰阿扎德大学食品卫生系,科学与研究分部(e-mail: Saaa4824@gmail.com)。对虹鳟鱼鱼片进行水提液和乙醇提液处理,计算其细菌总数。以100 μl水提液和醇提液稀释10倍,测定最小抑菌浓度,以虹鳟鱼鱼片25 g为最佳水提液和醇提液。结果表明,水提液和醇提液对金黄色葡萄球菌的抑菌效果最佳,分别为3.125 mg ml和6.25 mg ml,随后对鱼体进行处理,两种提取液对金黄色葡萄球菌的抑菌效果均优于对照组。因此,我们认为这种植物对提高鱼类的质量和保质期具有如此宝贵的作用。
{"title":"Antimicrobial efficiency of Allium atroviolaceum extract on Rainbow trout in different temperature and storage time","authors":"B. Hafeznia, S. Anvar, S. Kakookali, N. Choobkar","doi":"10.29252/IJAAH.4.2.86","DOIUrl":"https://doi.org/10.29252/IJAAH.4.2.86","url":null,"abstract":"Foodborne diseases in nature are usually infectious or toxic, caused by bacteria, viruses, parasites or chemicals, transmitted through food or water contaminated by the human body. To control the disease, as well as to ensure that the food is safe and healthy, its risks must be checked, detected and controlled. The goals of food industry are to produce healthy and high quality products that requires no contamination with bacteria. The use of natural preservatives such as herbal extracts and essential oils is recommended. Allium atroviolaceum is a family of Alliaceae and is a perennial herb which has a high nutritional value. The role of plant extracts has been evaluated as a preservative. Correspondence S.A.A Anvar, Department of Food Hygiene, Science and Research Branch, Islamic Azad University, Tehran, Iran (e-mail: Saaa4824@gmail.com ). The total number of bacteria calculated followed by treatment of the aqueous and ethanol extractions of the A. atroviolaceum on rainbow trout fish fillet. 100 μl of aqueous and alcoholic extracts followed by ten times dilution used to evaluate the minimum inhibitory concentration, then 25 grams of rainbow trout fillet was submerged with the best concentrations of aqueous and alcoholic extracts. The results illustrated that the best aqueous and alcoholic extractions against Staphylococcus aureus was 3.125 mg ml and 6.25 mg ml and followed by treatment on fish both type of extraction showed inhibition on growth of bacteria compare to control group. So we believe that this plant has such a valuable effect to improve the quality and shelf life of fish.","PeriodicalId":14506,"journal":{"name":"Iranian Journal of Aquatic Animal Health","volume":"79 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85927565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
期刊
Iranian Journal of Aquatic Animal Health
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