Heart failure (HF) incidence increases worldwide and is affected by various risk factors such as coronary artery disease, hypertension, obesity, and diabetes. Dietary recommendations for patients with HF have generally focused on sodium restriction; however, different nutritional approaches are considered in patients with a high risk of malnutrition due to the diuretic drugs they use. Omega-3 fatty acids obtained from aquatic organisms are essential regulators of cardiovascular health. There are different opinions on giving/consuming omega-3 fatty acids (and supplements) to improve the symptoms of heart failure. Although found mainly in oily fish, EPA and DHA, the marine n-3 polyunsaturated fatty acids are now found in commercially available supplements over the counter (as fish oils) or as concentrated pharmaceutical preparations. Fatty fish is the premier natural food source of EPA and DHA. In this study, we review the effects of omega-3 fatty acids, associated with low cardiovascular disease risk, on HF while trying to understand if it could ameliorate or exacerbate HF.
{"title":"Review Article: The importance of Omega-3 fatty acids in fish on human health","authors":"D. N. Kaplan, Z. Selamoğlu","doi":"10.52547/ijaah.7.2.1","DOIUrl":"https://doi.org/10.52547/ijaah.7.2.1","url":null,"abstract":"Heart failure (HF) incidence increases worldwide and is affected by various risk factors such as coronary artery disease, hypertension, obesity, and diabetes. Dietary recommendations for patients with HF have generally focused on sodium restriction; however, different nutritional approaches are considered in patients with a high risk of malnutrition due to the diuretic drugs they use. Omega-3 fatty acids obtained from aquatic organisms are essential regulators of cardiovascular health. There are different opinions on giving/consuming omega-3 fatty acids (and supplements) to improve the symptoms of heart failure. Although found mainly in oily fish, EPA and DHA, the marine n-3 polyunsaturated fatty acids are now found in commercially available supplements over the counter (as fish oils) or as concentrated pharmaceutical preparations. Fatty fish is the premier natural food source of EPA and DHA. In this study, we review the effects of omega-3 fatty acids, associated with low cardiovascular disease risk, on HF while trying to understand if it could ameliorate or exacerbate HF.","PeriodicalId":14506,"journal":{"name":"Iranian Journal of Aquatic Animal Health","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75180588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
During this research from April to August 2020, some 30 fish of Chalcalburnus chalcoides were caught from different parts of Sefidrood by cast nets. The fish were anesthetized using 25 mg/l of clove flower extract. In order to identify the parasites, blood, eyes, various internal organs were sampled. All of the isolated parasites were stained and clarified using Hematoxylin and Eosin stain and valid parasitology keys. Examination of internal parts of Chalcalburnus chalcoides revealed varying degrees of infestation by different parasitic species, including fish eyes infection by trematodes Diplostomum spathaceum , (infestation percentage 53.3%). Meanwhile, blood was affected mainly by Trypanosome percae (13.3%), Cryptobia sp. abdominal cavity by Eustrongylides excisus (20%), Clinostomum complanatum (13.3%), anisakis larvae (23.3%), Asymphylodora kubanicum (46.6%), Caryophylaelus laticeps Khavaia arminica metacercaria plerocercoid Ligula intestinalis Bothriocephalus acus (26%) and Corynosoma caspicum (30%). Almost all of the examined fish were found to have been infested by at least one or two parasite species. The new finding of this research is that isolation and identification of four parasites species namely as zoonosis Clinostomum complanatum , Eustrongylides excisus , Ligula intestinalis and Anisakis larvae are reported in this fish species for the first time.
{"title":"Research Article: Identification of Chalcalburnus chalcoides internal parasites in Sefidrood River, Guilan, Iran","authors":"M. Rahanandeh, B. Tizkar, M. Abedi","doi":"10.52547/ijaah.7.2.19","DOIUrl":"https://doi.org/10.52547/ijaah.7.2.19","url":null,"abstract":"During this research from April to August 2020, some 30 fish of Chalcalburnus chalcoides were caught from different parts of Sefidrood by cast nets. The fish were anesthetized using 25 mg/l of clove flower extract. In order to identify the parasites, blood, eyes, various internal organs were sampled. All of the isolated parasites were stained and clarified using Hematoxylin and Eosin stain and valid parasitology keys. Examination of internal parts of Chalcalburnus chalcoides revealed varying degrees of infestation by different parasitic species, including fish eyes infection by trematodes Diplostomum spathaceum , (infestation percentage 53.3%). Meanwhile, blood was affected mainly by Trypanosome percae (13.3%), Cryptobia sp. abdominal cavity by Eustrongylides excisus (20%), Clinostomum complanatum (13.3%), anisakis larvae (23.3%), Asymphylodora kubanicum (46.6%), Caryophylaelus laticeps Khavaia arminica metacercaria plerocercoid Ligula intestinalis Bothriocephalus acus (26%) and Corynosoma caspicum (30%). Almost all of the examined fish were found to have been infested by at least one or two parasite species. The new finding of this research is that isolation and identification of four parasites species namely as zoonosis Clinostomum complanatum , Eustrongylides excisus , Ligula intestinalis and Anisakis larvae are reported in this fish species for the first time.","PeriodicalId":14506,"journal":{"name":"Iranian Journal of Aquatic Animal Health","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85548813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study has investigated the impacts of biofloc on water quality, growth parameters, and whole-body composition of the Litopenaeus vannamei juveniles (initial average weight 5.23±0.20 g). Five experimental treatments were designed: Shrimp fed basal diet (Control), 5 and 10% wet biofloc-supplemented diets (W5 and W10), 5 and 10% dried biofloc-supplemented diets (D5 and D10) for 32 days. Experimental tanks (50 liters) as triplicate for each treatment, was stocked 12 shrimps. During the experiment, chemical and physical water parameters were examined and were not different statistically among experimental treatments. At the end of the experiment, in W10 treatment, the growth parameters were significantly higher than the control treatment. Also, in W10 and D5 treatments, feed conversion efficiency (FCE) and feed conversion ratio (FCR) were significantly better than the control. Compared to other treatments, significantly better protein efficiency ratio (PER) and lipid efficiency ratio (LER) were observed in W10 treatment. Results indicated that in W10 and D5 treatments, the protein, ash, and dry matter contents of the shrimp were significantly higher compared to the control treatment. Also, in W10, D5, and D10 treatments, carcass lipid and fiber contents were significantly higher compared to the control. Overall, the best performance was observed in shrimp fed on 10% wet biofloc-supplemented diet.
{"title":"Research Article: The effect of biofloc-supplemented diets on the Pacific white shrimp (Litopenaeus vannamei): Analysis of water quality, growth performance, and biochemical composition","authors":"M. Barzamini, M. Harsij, H. Adineh, H. Jafaryan","doi":"10.52547/ijaah.7.2.30","DOIUrl":"https://doi.org/10.52547/ijaah.7.2.30","url":null,"abstract":"This study has investigated the impacts of biofloc on water quality, growth parameters, and whole-body composition of the Litopenaeus vannamei juveniles (initial average weight 5.23±0.20 g). Five experimental treatments were designed: Shrimp fed basal diet (Control), 5 and 10% wet biofloc-supplemented diets (W5 and W10), 5 and 10% dried biofloc-supplemented diets (D5 and D10) for 32 days. Experimental tanks (50 liters) as triplicate for each treatment, was stocked 12 shrimps. During the experiment, chemical and physical water parameters were examined and were not different statistically among experimental treatments. At the end of the experiment, in W10 treatment, the growth parameters were significantly higher than the control treatment. Also, in W10 and D5 treatments, feed conversion efficiency (FCE) and feed conversion ratio (FCR) were significantly better than the control. Compared to other treatments, significantly better protein efficiency ratio (PER) and lipid efficiency ratio (LER) were observed in W10 treatment. Results indicated that in W10 and D5 treatments, the protein, ash, and dry matter contents of the shrimp were significantly higher compared to the control treatment. Also, in W10, D5, and D10 treatments, carcass lipid and fiber contents were significantly higher compared to the control. Overall, the best performance was observed in shrimp fed on 10% wet biofloc-supplemented diet.","PeriodicalId":14506,"journal":{"name":"Iranian Journal of Aquatic Animal Health","volume":"92 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83812336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study was conducted to evaluate the effect of different doses of GnRH on stress response in female koi carp ( Cyprinus carpio ). For this purpose, forty sexually mature female koi carp with an average weight of 102.05 ± 9.03 g were divided to four groups and fish received with a single intraperitoneal 0.9% NaCl with 20 mg kg -1 metoclopramide (C); 10 μg kg body weight -1 (BW) GnRH with 20 mg kg -1 metoclopramide (Gn10); 20 μg kg BW -1 GnRH with 20 mg kg -1 metoclopramide (Gn20); 50 μg kg BW -1 GnRH with 20 mg kg -1 metoclopramide (Gn50). Blood samples were collected before and after ovulation (approximately 24 h post-injection). The concentrations of plasma cortisol, lactate, and glucose were measured. Broodstocks who received GnRH spawned, while broodstocks of the group C did not spawn. The Gn10, Gn20, and Gn50 treatments led to significantly higher cortisol, lactate, and glucose concentration after ovulation compared to before injection ( p< 0.05 ). The present results showed that females are highly sensitive to manipulation during reproduction and higher levels of the hormone cause more stress broodstocks, so they must be held with minimal disorders, especially during spawning period.
本研究旨在探讨不同剂量的GnRH对雌性锦鲤应激反应的影响。将40尾平均体重为102.05±9.03 g的性成熟雌锦鲤分为4组，分别腹腔注射0.9% NaCl和20 mg kg -1甲氧氯普胺(C);10 μg kg体重-1 (BW) GnRH加20 mg kg -1甲氧氯普胺(Gn10);20 μg kg BW -1 GnRH加20 mg kg -1甲氧氯普胺(Gn20);50 μg kg BW -1 GnRH加20 mg kg -1甲氧氯普胺(Gn50)。在排卵前后(注射后约24小时)采集血样。测定血浆皮质醇、乳酸和葡萄糖浓度。接受GnRH的亲鱼产卵，而C组的亲鱼没有产卵。Gn10、Gn20和Gn50组小鼠排卵后皮质醇、乳酸和葡萄糖浓度显著高于注射前(p< 0.05)。目前的研究结果表明，雌性在繁殖过程中对操纵高度敏感，较高的激素水平会对亲鱼造成更大的压力，因此必须尽量减少对它们的干扰，特别是在产卵期。
{"title":"Research Article: The effect of different doses of GnRH on stress responses in female koi carp (Cyprinus carpio)","authors":"M. M. Eslami, S. Javadian, S. Bahram","doi":"10.52547/ijaah.7.2.61","DOIUrl":"https://doi.org/10.52547/ijaah.7.2.61","url":null,"abstract":"This study was conducted to evaluate the effect of different doses of GnRH on stress response in female koi carp ( Cyprinus carpio ). For this purpose, forty sexually mature female koi carp with an average weight of 102.05 ± 9.03 g were divided to four groups and fish received with a single intraperitoneal 0.9% NaCl with 20 mg kg -1 metoclopramide (C); 10 μg kg body weight -1 (BW) GnRH with 20 mg kg -1 metoclopramide (Gn10); 20 μg kg BW -1 GnRH with 20 mg kg -1 metoclopramide (Gn20); 50 μg kg BW -1 GnRH with 20 mg kg -1 metoclopramide (Gn50). Blood samples were collected before and after ovulation (approximately 24 h post-injection). The concentrations of plasma cortisol, lactate, and glucose were measured. Broodstocks who received GnRH spawned, while broodstocks of the group C did not spawn. The Gn10, Gn20, and Gn50 treatments led to significantly higher cortisol, lactate, and glucose concentration after ovulation compared to before injection ( p< 0.05 ). The present results showed that females are highly sensitive to manipulation during reproduction and higher levels of the hormone cause more stress broodstocks, so they must be held with minimal disorders, especially during spawning period.","PeriodicalId":14506,"journal":{"name":"Iranian Journal of Aquatic Animal Health","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83642852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Arayesh, S. Motahari, R. Kazempoor, M. Farahani
The herbicides used in agriculture threaten aquatic ecosystems and biodiversity on a global scale. There are several reports on the residues of currently used herbicides in the tissues of aquatic organisms. This study aimed to determine the effect of concentration on the accumulation of Butachlor in liver tissue in zebrafish fish exposed to sub-lethal concentrations of the toxin. In this study, we investigated the losses and residual toxins in the water and the liver of the zebrafish exposed to sub-lethal concentrations of Butachlor herbicide. This study was performed on 680 zebrafish. Initially, to determine the LC 50 -96 h value, a preliminary pilot study was performed, according to which, the main experiment was then conducted considering four treatments each with three replicates for 30 days. The experimental groups included T1 (exposure to 40% of the LC 50 -96 h concentration of Butachlor), T2 (exposure to 60% of LC 50 -96 h), T3 (exposure to 80% of LC 50 -96 h), and control (C). Mortality was recorded daily. Sampling was conducted from water and liver tissues on the first day and the days 15 and 30 to determine Butachlor residues using gas chromatography (GC). The results showed that Butachlor residue in water was associated with the concentration of the toxin and the exposure time, (T3>T2>T1 groups; p <0.05 ). According to the results, the highest mortality and residual Butachlor in the liver tissue were related to the T2>T3>T1> C groups, respectively, on days 15 and 30 after exposure ( p <0.05 ). According to the results of this study, Butachlor herbicide can accumulate in liver tissues of zebrafish even when it is used in low concentrations. Also the behavioral and clinical features following Butachlor use included restlessness, rapid respiration, air swallowing at the surface of the water, loss of balance, and disoriented swimming was observed. Regarding the importance of fish as the protein source in humans’ food, this phenomenon can be a potential threat to human health. Therefore, it is necessary to reduce the application of this toxin and replace it with alternative compounds.
{"title":"Research Article: Bioaccumulation of different concentrations of Butachlor in the Zebrafish (Danio rerio)","authors":"P. Arayesh, S. Motahari, R. Kazempoor, M. Farahani","doi":"10.52547/ijaah.7.2.7","DOIUrl":"https://doi.org/10.52547/ijaah.7.2.7","url":null,"abstract":"The herbicides used in agriculture threaten aquatic ecosystems and biodiversity on a global scale. There are several reports on the residues of currently used herbicides in the tissues of aquatic organisms. This study aimed to determine the effect of concentration on the accumulation of Butachlor in liver tissue in zebrafish fish exposed to sub-lethal concentrations of the toxin. In this study, we investigated the losses and residual toxins in the water and the liver of the zebrafish exposed to sub-lethal concentrations of Butachlor herbicide. This study was performed on 680 zebrafish. Initially, to determine the LC 50 -96 h value, a preliminary pilot study was performed, according to which, the main experiment was then conducted considering four treatments each with three replicates for 30 days. The experimental groups included T1 (exposure to 40% of the LC 50 -96 h concentration of Butachlor), T2 (exposure to 60% of LC 50 -96 h), T3 (exposure to 80% of LC 50 -96 h), and control (C). Mortality was recorded daily. Sampling was conducted from water and liver tissues on the first day and the days 15 and 30 to determine Butachlor residues using gas chromatography (GC). The results showed that Butachlor residue in water was associated with the concentration of the toxin and the exposure time, (T3>T2>T1 groups; p <0.05 ). According to the results, the highest mortality and residual Butachlor in the liver tissue were related to the T2>T3>T1> C groups, respectively, on days 15 and 30 after exposure ( p <0.05 ). According to the results of this study, Butachlor herbicide can accumulate in liver tissues of zebrafish even when it is used in low concentrations. Also the behavioral and clinical features following Butachlor use included restlessness, rapid respiration, air swallowing at the surface of the water, loss of balance, and disoriented swimming was observed. Regarding the importance of fish as the protein source in humans’ food, this phenomenon can be a potential threat to human health. Therefore, it is necessary to reduce the application of this toxin and replace it with alternative compounds.","PeriodicalId":14506,"journal":{"name":"Iranian Journal of Aquatic Animal Health","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84510544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study aimed to investigate the effects of complementary rainbow trout diets with different concentrations of Celmanax® (active compounds of Saccharomyces cerevisiae with mannan-oligosaccharide (MOS)) on immune responses, pressure resistance and resistance to Yersiniosis. Rainbow trout were fed with a diet containing various concentration levels of S.cerevisiae with MOS (prebiotic) (0, 0.1, 0.5 and 1%) for 60 days. While evaluating some of the parameters of the immune system, the blood samples were prepared from the tuber stem vein every 30 days. On day sixty of the study, various stress tests including temperature increases, hypoxia and induction of experimental disease with Yersinia ruckeri were also performed in all the experimental groups. Results showed that alternative complement pathway and total antibody were significantly elevated by diets containing different concentrations of prebiotic and the found be 0.1% ( p<0.05 ). However, the results of environmental pressures and exposure to bacteria showed that rainbow trout resistance was increased with different concentrations of prebiotic and the effective supplementation diet concentration was also 0.1% ( p<0.05 ). Based on these findings, it is suggested that to increase and enhance immunity and improve rainbow trout resistance, it would be appropriate to add 0.1% concentration of prebiotic in the diet.
{"title":"Research Article: Effects of diet supplementation with different level of Celmanax® (Saccharomyces cerevisiae cell wall with Mannan-Oligosaccharides) on health, environmental stress and Yersiniosis in Oncorhynchus mykiss","authors":"A. Khodadadi, H. Malekinejad, M. Hosseini","doi":"10.52547/ijaah.7.2.44","DOIUrl":"https://doi.org/10.52547/ijaah.7.2.44","url":null,"abstract":"This study aimed to investigate the effects of complementary rainbow trout diets with different concentrations of Celmanax® (active compounds of Saccharomyces cerevisiae with mannan-oligosaccharide (MOS)) on immune responses, pressure resistance and resistance to Yersiniosis. Rainbow trout were fed with a diet containing various concentration levels of S.cerevisiae with MOS (prebiotic) (0, 0.1, 0.5 and 1%) for 60 days. While evaluating some of the parameters of the immune system, the blood samples were prepared from the tuber stem vein every 30 days. On day sixty of the study, various stress tests including temperature increases, hypoxia and induction of experimental disease with Yersinia ruckeri were also performed in all the experimental groups. Results showed that alternative complement pathway and total antibody were significantly elevated by diets containing different concentrations of prebiotic and the found be 0.1% ( p<0.05 ). However, the results of environmental pressures and exposure to bacteria showed that rainbow trout resistance was increased with different concentrations of prebiotic and the effective supplementation diet concentration was also 0.1% ( p<0.05 ). Based on these findings, it is suggested that to increase and enhance immunity and improve rainbow trout resistance, it would be appropriate to add 0.1% concentration of prebiotic in the diet.","PeriodicalId":14506,"journal":{"name":"Iranian Journal of Aquatic Animal Health","volume":"45 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91380686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This research was conducted to evaluate the effect of different levels of Alphamune prebiotic on growth performance and blood chemistry of rainbow trout (Oncorhynchus mykiss) juvenile. Rainbow trout with an initial weight of 16.46±0.09 g were randomly assigned to four dietary treatments for eight weeks. The dietary treatment was: 0 (ALP0), 1 (ALP1), 1.5 (ALP1.5), and 2 (ALP2) g of Alphamune prebiotic/kg of basal diet. The results showed that there was no significant difference in survival rate and SGR at the end of the trial. A significant difference was observed in the final weight and weight gain, and the highest one was obtained in ALP1.5 treatment (p<0.05). Food conversion rate (FCR) was affected by Alphamune levels and the lowest FCR was observed in ALP1.5 treatment. Blood biochemistry assay revealed that glucose, triglyceride, and cholesterol were not influenced by Alphamune's different levels. Corresponding author's Email: Ro.javadian@gmail.com. There were significant differences in IgM and total protein and the highest value was obtained in ALP2. These results showed that 1.5% Alphamune prebiotic (ALP1.5) had a positive effect on growth performance and biochemical parameters of rainbow trout juveniles. Keyword: Alphamune, Prebiotic, Growth, Oncorhynchus mykiss
{"title":"Research Article: Evaluation of feed efficiency, growth and biochemical parameters of rainbow trout (Oncorhynchus mikyss) juveniles fed with different levels of Alphamune prebiotic","authors":"H. Kanani, S. Javadian, S. Bahram","doi":"10.52547/ijaah.7.1.47","DOIUrl":"https://doi.org/10.52547/ijaah.7.1.47","url":null,"abstract":"This research was conducted to evaluate the effect of different levels of Alphamune prebiotic on growth performance and blood chemistry of rainbow trout (Oncorhynchus mykiss) juvenile. Rainbow trout with an initial weight of 16.46±0.09 g were randomly assigned to four dietary treatments for eight weeks. The dietary treatment was: 0 (ALP0), 1 (ALP1), 1.5 (ALP1.5), and 2 (ALP2) g of Alphamune prebiotic/kg of basal diet. The results showed that there was no significant difference in survival rate and SGR at the end of the trial. A significant difference was observed in the final weight and weight gain, and the highest one was obtained in ALP1.5 treatment (p<0.05). Food conversion rate (FCR) was affected by Alphamune levels and the lowest FCR was observed in ALP1.5 treatment. Blood biochemistry assay revealed that glucose, triglyceride, and cholesterol were not influenced by Alphamune's different levels. Corresponding author's Email: Ro.javadian@gmail.com. There were significant differences in IgM and total protein and the highest value was obtained in ALP2. These results showed that 1.5% Alphamune prebiotic (ALP1.5) had a positive effect on growth performance and biochemical parameters of rainbow trout juveniles. Keyword: Alphamune, Prebiotic, Growth, Oncorhynchus mykiss","PeriodicalId":14506,"journal":{"name":"Iranian Journal of Aquatic Animal Health","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89254257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Mohamed, R. Abdi, M. Ronagh, M. A. Salari - Ali Abadi, Z. Basir
Three species of fish including, macroscopic scaled fish as Otolithes ruber, microscopic scaled sturgeon as Huso huso and free scaled cat fish as Pangasius hypophthalmus were prepared and specimen of dorsal of head and caudal peduncle were carried out. Routine procedures of tissues preparation followed and paraffin sections stained with (H&E) and (PAS). Results showed, epidermis formed non keratinized stratified squamous epithelium with epidermis, lymphocytes, goblet cells, taste bud and club cells. The epidermis thickness of the head skin was higher than that of the caudal peduncle, as demonstrated by image analysis using light microscopy. Goblet cells were along the superficial cells layers and their distributions were varied. Corresponding author's Email: abdir351@gmail.com In histomorphometric studies by PAS staining the highest number of these cells were seen in head of O. ruber and the lowest were seen in the caudal peduncle of P. hypophthalmus. Most of them were seen from the middle to surface layer of the epidermis. Club cells, with large nucleus, mostly evident in the deep and middle layer of the epidermis, being the largest cells within the epithelium. The highest numbers of these cells (61.8 ± 2.16) were found in head region of P. hypophthalmus. Taste buds as a sensory organ were not seen in caudal peduncle of O. ruber and H. huso. Based on the results of this study, epidermis had similarities in cell type and differences in their numbers that could be justified by the presence or absence of scales.
{"title":"Research Article: Comparative histomorphology of epidermis of head and caudal peduncle in Otolithes ruber, Huso huso and Pangasius hypophthalmus fish","authors":"M. Mohamed, R. Abdi, M. Ronagh, M. A. Salari - Ali Abadi, Z. Basir","doi":"10.52547/ijaah.7.1.10","DOIUrl":"https://doi.org/10.52547/ijaah.7.1.10","url":null,"abstract":"Three species of fish including, macroscopic scaled fish as Otolithes ruber, microscopic scaled sturgeon as Huso huso and free scaled cat fish as Pangasius hypophthalmus were prepared and specimen of dorsal of head and caudal peduncle were carried out. Routine procedures of tissues preparation followed and paraffin sections stained with (H&E) and (PAS). Results showed, epidermis formed non keratinized stratified squamous epithelium with epidermis, lymphocytes, goblet cells, taste bud and club cells. The epidermis thickness of the head skin was higher than that of the caudal peduncle, as demonstrated by image analysis using light microscopy. Goblet cells were along the superficial cells layers and their distributions were varied. Corresponding author's Email: abdir351@gmail.com In histomorphometric studies by PAS staining the highest number of these cells were seen in head of O. ruber and the lowest were seen in the caudal peduncle of P. hypophthalmus. Most of them were seen from the middle to surface layer of the epidermis. Club cells, with large nucleus, mostly evident in the deep and middle layer of the epidermis, being the largest cells within the epithelium. The highest numbers of these cells (61.8 ± 2.16) were found in head region of P. hypophthalmus. Taste buds as a sensory organ were not seen in caudal peduncle of O. ruber and H. huso. Based on the results of this study, epidermis had similarities in cell type and differences in their numbers that could be justified by the presence or absence of scales.","PeriodicalId":14506,"journal":{"name":"Iranian Journal of Aquatic Animal Health","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90281745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Bazari Moghaddam, M. Sharif rohani, M. Haghighi
Today, nanotechnology is considered as a priority and strategic technology for all countries due to its wide range of applications. The present study was performed to evaluate the effects of various levels of dried Aloe vera extract nanoparticles on some hematological parameters, liver enzymes and immune responses of Siberian sturgeon (Acipencer baerii). Three hundred sixty Acipenser baerii (with mean weight of 10.95 ± 0.04 g), were randomly divided into three treatment groups including 0.5%, 1% and 1.5% of nanoparticles of dried Aloe vera extract and one control group (without any additive), each in three replicates, stored in twelve tanks of 500-liter fiberglass with 350 liters water. Water physicochemical parameters were recorded daily. Nanoparticles of dried A. vera extract in a ratio of 0.5%, 1%, and 1.5% were added to the treatment groups diet for 2 months. Corresponding author's E-mail: Soheilbm274@gmail.com. At the end of the first and second months of rearing, the necessary samplings for hematology and serology studies were done. The results of this study showed that the amounts of RBC, Hb, Hct, MCV, WBC, lysozyme, ACH50 and IgM were significantly different in the treatment groups compared with the control group at the end of the study (p<0.05). There were no significant differences in liver enzymes including ALT, AST and ALP between treatments and control groups (p>0.05). In conclusion the results have shown that use of nanoparticles of dried A. vera extract had the impact of immune function booster and the usage of this nanoparticle mainly at the level of 1% in the diet can be used as a safety stimulant for Siberian sturgeon.
{"title":"Research Article: Effects of nanoparticles of dried Aloe vera extract on some of the hematological parameters, liver enzymes and immune responses in Siberian sturgeon (Acipenser baerii)","authors":"S. Bazari Moghaddam, M. Sharif rohani, M. Haghighi","doi":"10.52547/ijaah.7.1.56","DOIUrl":"https://doi.org/10.52547/ijaah.7.1.56","url":null,"abstract":"Today, nanotechnology is considered as a priority and strategic technology for all countries due to its wide range of applications. The present study was performed to evaluate the effects of various levels of dried Aloe vera extract nanoparticles on some hematological parameters, liver enzymes and immune responses of Siberian sturgeon (Acipencer baerii). Three hundred sixty Acipenser baerii (with mean weight of 10.95 ± 0.04 g), were randomly divided into three treatment groups including 0.5%, 1% and 1.5% of nanoparticles of dried Aloe vera extract and one control group (without any additive), each in three replicates, stored in twelve tanks of 500-liter fiberglass with 350 liters water. Water physicochemical parameters were recorded daily. Nanoparticles of dried A. vera extract in a ratio of 0.5%, 1%, and 1.5% were added to the treatment groups diet for 2 months. Corresponding author's E-mail: Soheilbm274@gmail.com. At the end of the first and second months of rearing, the necessary samplings for hematology and serology studies were done. The results of this study showed that the amounts of RBC, Hb, Hct, MCV, WBC, lysozyme, ACH50 and IgM were significantly different in the treatment groups compared with the control group at the end of the study (p<0.05). There were no significant differences in liver enzymes including ALT, AST and ALP between treatments and control groups (p>0.05). In conclusion the results have shown that use of nanoparticles of dried A. vera extract had the impact of immune function booster and the usage of this nanoparticle mainly at the level of 1% in the diet can be used as a safety stimulant for Siberian sturgeon.","PeriodicalId":14506,"journal":{"name":"Iranian Journal of Aquatic Animal Health","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76292925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microalgae contain many valuable biological compounds, so their commercial production is of great importance. One of the important challenges in microalga production is their high cost associated with their harvesting processes. Therefore, finding efficient and cost-effective technologies for algae biomass separation is essential for achieving an operational process. The study was conducted to find the influence of pH variation from 4 to 11 on harvesting efficiency and fatty acids component of marine microalgae Thalassiosira sp under laboratory condition. The NaOH and HCl were added to adjust pH value in each treatment. Data was presented as mean ± SD. Comparison between treatments was performed using one-way analysis of variance ANOVA. The results showed that with increasing the pH up to 10, flocculation processes (92%) and concentration factor (20) increased significantly. Corresponding author's Email: paria.akbary@gmail.com While, lowering the pH from 8.2 to 4 was not effective in isolating algal biomass. In the next stage, the biomass fatty acids content was measured by both alkaline pH induction method and the centrifuge method. Analyzes showed that the percentage of lipid and saturated and unsaturated fatty acids in biomass collected by alkaline pH induction technique was lower than the centrifugation method. In general, the results of the present study showed that clotting in alkaline pH up to 10 can be suggested as a suitable method for harvesting of Thalassiosira sp microalgae, and if the production of polyunsaturated acids is considered, of consuming high amounts of sodium hydroxide should be avoided.
{"title":"Research Article: Effects of pH-induced technique on flocculation efficiency and fatty acids profile of marine microalgae, Thalassiosira sp","authors":"M. Hobbi, P. Akbary, Z. Aminikhoei","doi":"10.52547/ijaah.7.1.21","DOIUrl":"https://doi.org/10.52547/ijaah.7.1.21","url":null,"abstract":"Microalgae contain many valuable biological compounds, so their commercial production is of great importance. One of the important challenges in microalga production is their high cost associated with their harvesting processes. Therefore, finding efficient and cost-effective technologies for algae biomass separation is essential for achieving an operational process. The study was conducted to find the influence of pH variation from 4 to 11 on harvesting efficiency and fatty acids component of marine microalgae Thalassiosira sp under laboratory condition. The NaOH and HCl were added to adjust pH value in each treatment. Data was presented as mean ± SD. Comparison between treatments was performed using one-way analysis of variance ANOVA. The results showed that with increasing the pH up to 10, flocculation processes (92%) and concentration factor (20) increased significantly. Corresponding author's Email: paria.akbary@gmail.com While, lowering the pH from 8.2 to 4 was not effective in isolating algal biomass. In the next stage, the biomass fatty acids content was measured by both alkaline pH induction method and the centrifuge method. Analyzes showed that the percentage of lipid and saturated and unsaturated fatty acids in biomass collected by alkaline pH induction technique was lower than the centrifugation method. In general, the results of the present study showed that clotting in alkaline pH up to 10 can be suggested as a suitable method for harvesting of Thalassiosira sp microalgae, and if the production of polyunsaturated acids is considered, of consuming high amounts of sodium hydroxide should be avoided.","PeriodicalId":14506,"journal":{"name":"Iranian Journal of Aquatic Animal Health","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77391189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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