Maria Fransiska Priska Yulianti, Anita Lidesna Shinta Amat, R. Hutasoit, P. Pakan
Background: Propionibacterium acnes is a bacterium found on the skin that plays a significant role in acne vulgaris. The inappropriate use of antibiotics can lead to resistance, necessitating the search for alternative therapies from plants with high antibacterial potential. One such plant is jamblang (Syzygium cumini), which has demonstrated antibacterial properties. Objective: This research aims to test the antibacterial activity of jamblang leaf extract against the growth of P. acnes. Method: The study employed an experimental design with a posttest-only control group design. The treatment groups consisted of a positive control (clindamycin), a negative control (sterile distilled water), and jamblang leaf extract groups with concentrations of 100%, 50%, 25%, 12.5%, and 6.25% (n=4). Data analysis was conducted using the One-Way ANOVA statistical test. Results: The jamblang leaf extract exhibited antibacterial potential against the growth of P. acnes. The extract showed strong antibacterial activity at concentrations of 100%, 50%, 25%, and 12.5%, and moderate antibacterial activity at a concentration of 6.25%. Conclusion: This suggests that jamblang leaf extract could be a promising alternative therapy for treating acne vulgaris, offering a natural solution to combat antibiotic resistance.
{"title":"Antibacterial activity of jamblang leaf ethanol extract (Syzygium cumini) against the growth of Propionibacterium acnes","authors":"Maria Fransiska Priska Yulianti, Anita Lidesna Shinta Amat, R. Hutasoit, P. Pakan","doi":"10.32889/actabiona.161","DOIUrl":"https://doi.org/10.32889/actabiona.161","url":null,"abstract":"Background: Propionibacterium acnes is a bacterium found on the skin that plays a significant role in acne vulgaris. The inappropriate use of antibiotics can lead to resistance, necessitating the search for alternative therapies from plants with high antibacterial potential. One such plant is jamblang (Syzygium cumini), which has demonstrated antibacterial properties. \u0000Objective: This research aims to test the antibacterial activity of jamblang leaf extract against the growth of P. acnes. \u0000Method: The study employed an experimental design with a posttest-only control group design. The treatment groups consisted of a positive control (clindamycin), a negative control (sterile distilled water), and jamblang leaf extract groups with concentrations of 100%, 50%, 25%, 12.5%, and 6.25% (n=4). Data analysis was conducted using the One-Way ANOVA statistical test. \u0000Results: The jamblang leaf extract exhibited antibacterial potential against the growth of P. acnes. The extract showed strong antibacterial activity at concentrations of 100%, 50%, 25%, and 12.5%, and moderate antibacterial activity at a concentration of 6.25%. \u0000Conclusion: This suggests that jamblang leaf extract could be a promising alternative therapy for treating acne vulgaris, offering a natural solution to combat antibiotic resistance.","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"35 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141651041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Syazili Mustofa, Ilma Puteri Hutami, Dwi Sarwindah
Background: The therapeutic properties of the ethanol extract derived from the stem bark of Rhizophora apiculata have been investigated. Nevertheless, there is a lack of studies regarding its acute toxicity. Objective: This research aims to evaluate the toxicity of ethanolic extract of R. apiculata bark by examining histological changes in the liver and kidney of rats. Method: The ethanol extract of R. apiculata bark was administered using a fixed-dose approach in preliminary and primary tests, focusing on the kidneys and liver of male Sprague Dawley rats. Four treatment groups received doses of 5, 50, 300, and 2000 mg/kg BW, while one group served as the control. An uninterrupted observation period of 14 days was conducted to determine any indications of acute toxicity in these animals. On the fifteenth day, the rats were terminated. Results: This study indicated the absence of any toxic manifestations, such as tremors, excessive salivation, convulsions, coma, and mortality. Nevertheless, it caused histological damage to the rats’ liver and kidney when administered at doses 300 and 2000 mg/kg BW. Conclusion: While the administration of R. apiculata extracts at doses of 300 and 2000 mg/kgBW resulted in histological damage to the kidneys and livers of the rats, it did not induce any immediate symptoms.
{"title":"Acute toxicity test of Rhizophora apiculata bark extract on rat liver and kidney histology using fixed dose method","authors":"Syazili Mustofa, Ilma Puteri Hutami, Dwi Sarwindah","doi":"10.32889/actabioina.144","DOIUrl":"https://doi.org/10.32889/actabioina.144","url":null,"abstract":"Background: The therapeutic properties of the ethanol extract derived from the stem bark of Rhizophora apiculata have been investigated. Nevertheless, there is a lack of studies regarding its acute toxicity. \u0000Objective: This research aims to evaluate the toxicity of ethanolic extract of R. apiculata bark by examining histological changes in the liver and kidney of rats. \u0000Method: The ethanol extract of R. apiculata bark was administered using a fixed-dose approach in preliminary and primary tests, focusing on the kidneys and liver of male Sprague Dawley rats. Four treatment groups received doses of 5, 50, 300, and 2000 mg/kg BW, while one group served as the control. An uninterrupted observation period of 14 days was conducted to determine any indications of acute toxicity in these animals. On the fifteenth day, the rats were terminated. \u0000Results: This study indicated the absence of any toxic manifestations, such as tremors, excessive salivation, convulsions, coma, and mortality. Nevertheless, it caused histological damage to the rats’ liver and kidney when administered at doses 300 and 2000 mg/kg BW. \u0000Conclusion: While the administration of R. apiculata extracts at doses of 300 and 2000 mg/kgBW resulted in histological damage to the kidneys and livers of the rats, it did not induce any immediate symptoms.","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":" 20","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141671215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. K. Widiasri, Anysah Nur Fauziyah, F. Alamsyah, R. Pratiwi
Background: Cancer therapy based on alternating current electric field exposure, Electro-Capacitive Cancer Therapy (ECCT), has been pre-clinically tested for its effectiveness in breast tumor inhibition. However, concerns regarding the safety of electric field (EF) exposure to vital organs have been raised. In the kidney and spleen, IL-10 and TNF-α play roles in the response to inflammation. Objective: This research aimed to evaluate the safety of electric field exposure to vital organs, by assessing the expression of IL-10 and TNF-α mRNA in the kidney and spleen of rats, both with and without breast tumors. Methods: Female rats were divided into four groups; non-induction-non-therapy (NINT), non-induction-therapy (NIT), induction-non-therapy (INT), and induction-therapy (IT). Rats were induced by 7,12-dimethylbenz[a]anthracene (DMBA) at a dosage 20 mg/Kg body weight and exposed to electric fields for 10 hours, followed by a 2-hour rest period. Total RNA from the kidney and spleen was isolated, cDNA was synthesized by reverse transcription PCR, and analysis of IL-10 and TNF-α mRNA expression was performed by RT-qPCR method. Data were analyzed using the Livak formula, GraphPad Prism 9.0 software, and significance test ANOVA (α = 0.05). Results: Alternating current electric field exposure had no effects on the expression of TNF-α and IL-10 mRNA in the kidney and spleen of rats. Specifically, no effect was observed on TNF-α mRNA expression in all groups, and there was no effect on IL-10 mRNA expression in the spleen. Only the INT group showed a significant increase in IL-10 mRNA expression in the kidney (p < 0.05). Conclusion: Exposure of an alternating current electric field did not affect the relative mRNA expression of TNF-α and IL-10 in the kidneys and spleen of rats.
{"title":"Relative expression of IL-10 and TNF-α mRNA of kidney and spleen tissues of rat with and without mammary tumor after exposed to alternating current electric field","authors":"N. K. Widiasri, Anysah Nur Fauziyah, F. Alamsyah, R. Pratiwi","doi":"10.32889/actabioina.107","DOIUrl":"https://doi.org/10.32889/actabioina.107","url":null,"abstract":"Background: Cancer therapy based on alternating current electric field exposure, Electro-Capacitive Cancer Therapy (ECCT), has been pre-clinically tested for its effectiveness in breast tumor inhibition. However, concerns regarding the safety of electric field (EF) exposure to vital organs have been raised. In the kidney and spleen, IL-10 and TNF-α play roles in the response to inflammation. \u0000Objective: This research aimed to evaluate the safety of electric field exposure to vital organs, by assessing the expression of IL-10 and TNF-α mRNA in the kidney and spleen of rats, both with and without breast tumors. \u0000Methods: Female rats were divided into four groups; non-induction-non-therapy (NINT), non-induction-therapy (NIT), induction-non-therapy (INT), and induction-therapy (IT). Rats were induced by 7,12-dimethylbenz[a]anthracene (DMBA) at a dosage 20 mg/Kg body weight and exposed to electric fields for 10 hours, followed by a 2-hour rest period. Total RNA from the kidney and spleen was isolated, cDNA was synthesized by reverse transcription PCR, and analysis of IL-10 and TNF-α mRNA expression was performed by RT-qPCR method. Data were analyzed using the Livak formula, GraphPad Prism 9.0 software, and significance test ANOVA (α = 0.05). \u0000Results: Alternating current electric field exposure had no effects on the expression of TNF-α and IL-10 mRNA in the kidney and spleen of rats. Specifically, no effect was observed on TNF-α mRNA expression in all groups, and there was no effect on IL-10 mRNA expression in the spleen. Only the INT group showed a significant increase in IL-10 mRNA expression in the kidney (p < 0.05). \u0000Conclusion: Exposure of an alternating current electric field did not affect the relative mRNA expression of TNF-α and IL-10 in the kidneys and spleen of rats.","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127196965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Heme is a compound consisting of an iron (Fe) atom bound to a pyrrole ring forming protoporphyrin IX (PPIX). Protoporphyrin combines with a protein-forming hemoprotein compound that plays an essential role in oxygen-binding and transport as well as in the process of energy production in the mitochondria. Some cancer cells have more heme biosynthesis than normal cells, which is thought to be linked to more cancer cell growth. Inhibition of heme biosynthesis in some cancer cells leads to decreased cell proliferation. This review article discusses the synthesis of heme, the role of heme in energy metabolism, which is needed for cell proliferation, the inhibition of heme synthesis and its effect on cancer cell proliferation, and the possibility of the inhibition of heme biosynthesis as an approach in therapy of cancer in the future.
{"title":"Is heme biosynthesis influenced the mitochondrial function and cell proliferation in cancer?","authors":"Raisa Nauli, S. A. Jusman","doi":"10.32889/actabioina.65","DOIUrl":"https://doi.org/10.32889/actabioina.65","url":null,"abstract":"Heme is a compound consisting of an iron (Fe) atom bound to a pyrrole ring forming protoporphyrin IX (PPIX). Protoporphyrin combines with a protein-forming hemoprotein compound that plays an essential role in oxygen-binding and transport as well as in the process of energy production in the mitochondria. Some cancer cells have more heme biosynthesis than normal cells, which is thought to be linked to more cancer cell growth. Inhibition of heme biosynthesis in some cancer cells leads to decreased cell proliferation. This review article discusses the synthesis of heme, the role of heme in energy metabolism, which is needed for cell proliferation, the inhibition of heme synthesis and its effect on cancer cell proliferation, and the possibility of the inhibition of heme biosynthesis as an approach in therapy of cancer in the future. \u0000 \u0000 \u0000 \u0000 ","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117022046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tri Wistya, Utami Pranita, Aritrina Laode, Kardin Sukurni, Andi Noor, Kholidha Syarifin, M. Subangkit
Background: Dillenia serrata (locally known as singi) is a natural product with the potential to improve liver function in hypercholesterolemia, due to anticholesterol and antioxidant properties. Objective: This study aimed to investigate the effect of D. serrata on the histopathological features of rat livers induced by a high-fat diet. Methods: This study used a quasi-experimental in vivo with a post-test-only control group design. Rats were divided into four groups: normal, high-fat diet, as well as D. serrata, and simvastatin treatment. Hypercholesterolemia was induced by a high-fat diet for two weeks. Rat liver tissues were analyzed histologically using Hematoxylin-Eosin (HE) staining, and were observed under a light microscope at 40x magnification in five wide fields of view. Results: The high-fat diet group had the most adipose cells, while the D. serrata group had the least. Statistical analysis demonstrated a significant difference between the high-fat diet group and the other groups (p < 0.05). Interestingly, no significant difference between the D. serrata group and either the normal or simvastatin group (p > 0.05), suggesting the treatment of fruit may restore liver function comparable to normal and simvastatin group. Conclusion: The D. serrata fruit reduces the number of fat cells in the histopathology of rats induced with the high-fat diet.
{"title":"Histopathological analysis of the liver in hypercholesterolemia rats treated with Dillenia serrata fruits","authors":"Tri Wistya, Utami Pranita, Aritrina Laode, Kardin Sukurni, Andi Noor, Kholidha Syarifin, M. Subangkit","doi":"10.32889/actabioina.56","DOIUrl":"https://doi.org/10.32889/actabioina.56","url":null,"abstract":"Background: Dillenia serrata (locally known as singi) is a natural product with the potential to improve liver function in hypercholesterolemia, due to anticholesterol and antioxidant properties. \u0000Objective: This study aimed to investigate the effect of D. serrata on the histopathological features of rat livers induced by a high-fat diet. \u0000Methods: This study used a quasi-experimental in vivo with a post-test-only control group design. Rats were divided into four groups: normal, high-fat diet, as well as D. serrata, and simvastatin treatment. Hypercholesterolemia was induced by a high-fat diet for two weeks. Rat liver tissues were analyzed histologically using Hematoxylin-Eosin (HE) staining, and were observed under a light microscope at 40x magnification in five wide fields of view. \u0000Results: The high-fat diet group had the most adipose cells, while the D. serrata group had the least. Statistical analysis demonstrated a significant difference between the high-fat diet group and the other groups (p < 0.05). Interestingly, no significant difference between the D. serrata group and either the normal or simvastatin group (p > 0.05), suggesting the treatment of fruit may restore liver function comparable to normal and simvastatin group. \u0000Conclusion: The D. serrata fruit reduces the number of fat cells in the histopathology of rats induced with the high-fat diet. \u0000 \u0000 \u0000 \u0000 ","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125507407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Free radicals are atoms or molecules with one or more unpaired electrons. Lead acetate has been reported to increase the presence of free radicals in the body. Objective: This study aims to investigate the effect of oral administration of jamblang (Syzygium cumini) leaf extract on hydrogen peroxide (H2O2) serum levels in rats induced with lead acetate. Methods: A total of 24 male rats were divided into three groups: the negative control, the positive control, and the treatment group. The negative control group received a standard diet, the positive control group received lead acetate at a dose of 40 mg/kg body weight, and the treatment group received lead acetate at the same dose along with jamblang leaf extract at a dose of 150 mg/kg body weight. Lead acetate and jamblang leaf extract administration was carried out for 30 days. Afterward, serum H2O2 levels were examined using the colorimetry method. Results: Results revealed that H2O2 levels in the negative control, positive control, and treatment groups were 3.08±0.24, 4.94±0.75, and 3.44±0.65 nmol/L, respectively. Significant differences were observed between the groups, as well as between the negative control and positive control, and between the positive control and treatment group. Conclusion: The study showed that jamblang leaf extract can reduce hydrogen peroxide levels in Wistar rats treated with lead acetate, indicating its ability to address oxidative stress.
{"title":"Jamblang (Syzygium cumini) leaf extract decreased hydrogen peroxide in lead acetate-induced rats","authors":"R. Rita, El-Nagdy Sy, Endrinaldi","doi":"10.32889/actabioina.118","DOIUrl":"https://doi.org/10.32889/actabioina.118","url":null,"abstract":"Background: Free radicals are atoms or molecules with one or more unpaired electrons. Lead acetate has been reported to increase the presence of free radicals in the body. \u0000Objective: This study aims to investigate the effect of oral administration of jamblang (Syzygium cumini) leaf extract on hydrogen peroxide (H2O2) serum levels in rats induced with lead acetate. \u0000Methods: A total of 24 male rats were divided into three groups: the negative control, the positive control, and the treatment group. The negative control group received a standard diet, the positive control group received lead acetate at a dose of 40 mg/kg body weight, and the treatment group received lead acetate at the same dose along with jamblang leaf extract at a dose of 150 mg/kg body weight. Lead acetate and jamblang leaf extract administration was carried out for 30 days. Afterward, serum H2O2 levels were examined using the colorimetry method. \u0000Results: Results revealed that H2O2 levels in the negative control, positive control, and treatment groups were 3.08±0.24, 4.94±0.75, and 3.44±0.65 nmol/L, respectively. Significant differences were observed between the groups, as well as between the negative control and positive control, and between the positive control and treatment group. \u0000Conclusion: The study showed that jamblang leaf extract can reduce hydrogen peroxide levels in Wistar rats treated with lead acetate, indicating its ability to address oxidative stress.","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131623769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Singh, Saba Khan, M. Khan, H. Ahsan, Roshan Alam
Background: During pregnancy, the physiological production of reactive oxygen species (ROS) is associated with a variety of maternal, placental, and fetal developmental functions. These functions are disrupted by excessive amounts of ROS, resulting to pregnancy complications. Different stages of pregnancy require a balance between oxidant and antioxidant production. Objective: The aim of this study is to investigate the status of malondialdehyde (MDA) and catalase (CAT) activity in pregnant and age matched non-pregnant women. Methods: In this cross-sectional study, a total of 74 participants were enrolled, including 37 pregnant and 37 age-matched non-pregnant women. The age range of participants was 18 to 40 years. MDA and CAT levels were measured spectrophotometrically. A p-value 0.05 was statistically significant. Results: Compared to non-pregnant women, the mean plasma level of MDA was considerably elevated in pregnant women (p<0.0001). However, the mean level of CAT activity in pregnant women was significantly lower than in non-pregnant women (p<0.0001) In pregnant women, there is a significant strong negative correlation between MDA levels and CAT activity (p<0.01). Conclusion: Pregnant women have high level of oxidative stress, indicating that the pregnant women are more susceptible to oxidative damage and may develop pregnancy-associated complications.
{"title":"Estimation of malondialdehyde and catalase activity in pregnant women at IIMS&R Hospital, Lucknow, India","authors":"N. Singh, Saba Khan, M. Khan, H. Ahsan, Roshan Alam","doi":"10.32889/actabioina.89","DOIUrl":"https://doi.org/10.32889/actabioina.89","url":null,"abstract":"Background: During pregnancy, the physiological production of reactive oxygen species (ROS) is associated with a variety of maternal, placental, and fetal developmental functions. These functions are disrupted by excessive amounts of ROS, resulting to pregnancy complications. Different stages of pregnancy require a balance between oxidant and antioxidant production. \u0000Objective: The aim of this study is to investigate the status of malondialdehyde (MDA) and catalase (CAT) activity in pregnant and age matched non-pregnant women. \u0000Methods: In this cross-sectional study, a total of 74 participants were enrolled, including 37 pregnant and 37 age-matched non-pregnant women. The age range of participants was 18 to 40 years. MDA and CAT levels were measured spectrophotometrically. A p-value 0.05 was statistically significant. \u0000Results: Compared to non-pregnant women, the mean plasma level of MDA was considerably elevated in pregnant women (p<0.0001). However, the mean level of CAT activity in pregnant women was significantly lower than in non-pregnant women (p<0.0001) In pregnant women, there is a significant strong negative correlation between MDA levels and CAT activity (p<0.01). \u0000Conclusion: Pregnant women have high level of oxidative stress, indicating that the pregnant women are more susceptible to oxidative damage and may develop pregnancy-associated complications. \u0000 ","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"83 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131713784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Hardiany, Stephanie Gosal, Damayanti Angelina, Engelbert Julyan Gravianto, R. Antarianto
Background: Fasting may increase the activity of endogenous antioxidants and protect against oxidative stress. However, the effects of different fasting durations on the liver have not been reported. Objective: The purpose of this study is to determine the effect of intermittent and prolonged fasting on oxidative stress markers in the liver tissue and plasma of New Zealand White rabbits. Methods: New Zealand White rabbits were divided into three groups: control, intermittent fasting (IF), and prolonged fasting (PF), with each group consisting of five rabbits. The control group was provided with food ad libitum; the IF group fasted for 16 hours, while the PF group fasted for 40 hours, followed by an eight-hour non-fasting period for six days. In liver tissue and plasma, oxidative stress indicators (catalase, carbonyl, GSH) were evaluated. Results: In the IF group, liver GSH was significantly higher than in the control group. However, neither liver carbonyl nor catalase levels changed significantly in the IF group. In the IF group, plasma carbonyl was significantly lower than in the PF group. In addition, there was no significant differences between groups in plasma catalase and GSH levels. Conclusion: Intermittent fasting and prolonged fasting could significantly increase liver GSH levels of New Zealand White rabbits. In addition, intermittent fasting is more effective than prolonged fasting at preventing oxidative stress.
{"title":"The impact of fasting toward oxidative stress marker in the liver and plasma of new zealand white rabbit","authors":"N. Hardiany, Stephanie Gosal, Damayanti Angelina, Engelbert Julyan Gravianto, R. Antarianto","doi":"10.32889/actabioina.112","DOIUrl":"https://doi.org/10.32889/actabioina.112","url":null,"abstract":"Background: Fasting may increase the activity of endogenous antioxidants and protect against oxidative stress. However, the effects of different fasting durations on the liver have not been reported. \u0000Objective: The purpose of this study is to determine the effect of intermittent and prolonged fasting on oxidative stress markers in the liver tissue and plasma of New Zealand White rabbits. \u0000Methods: New Zealand White rabbits were divided into three groups: control, intermittent fasting (IF), and prolonged fasting (PF), with each group consisting of five rabbits. The control group was provided with food ad libitum; the IF group fasted for 16 hours, while the PF group fasted for 40 hours, followed by an eight-hour non-fasting period for six days. In liver tissue and plasma, oxidative stress indicators (catalase, carbonyl, GSH) were evaluated. \u0000Results: In the IF group, liver GSH was significantly higher than in the control group. However, neither liver carbonyl nor catalase levels changed significantly in the IF group. In the IF group, plasma carbonyl was significantly lower than in the PF group. In addition, there was no significant differences between groups in plasma catalase and GSH levels. \u0000Conclusion: Intermittent fasting and prolonged fasting could significantly increase liver GSH levels of New Zealand White rabbits. In addition, intermittent fasting is more effective than prolonged fasting at preventing oxidative stress.","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128393064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Dewi, Alexander Rafael Satyadharma, A. R. Danendra, Wardaya
Background: Hypobaric hypoxia is a state of decreased oxygen pressure at high altitudes that can lead to hypoxia and oxidative stress as a result. Skeletal muscle is one of the important organs that can be affected by oxidative stress and cause contractile dysfunction. Objective: This study aimed to evaluate the impact of intermittent hypobaric hypoxia on oxidative stress markers in rat skeletal muscle, by measuring malondialdehyde (MDA) and carbonyl levels. Methods: Twenty-five Wistar rats were allocated into five groups, including one control group and four hypoxic groups (I-IV). The hypoxic groups were exposed to an altitude of 25,000 feet for 5 minutes using hypobaric chamber in once (I), twice (II), three (III), and four (IV) times, with a 7-day interval period between exposures. The control group remained in normobaric conditions throughout the study. MDA levels were measured by thiobarbituric acid (TBA) test, while carbonyl levels were measured using 2,4-dinitrophenylhydrazine (DNPH) reagent. Results: The MDA level was significantly increased in group I compared to the control group (p=0.008). There were decreasing MDA levels in groups II, III, and IV compared to group I. The carbonyl level was significantly higher in group I than the control group (p=0.000), with an even higher level observed in group II. Although the carbonyl levels tended to decrease in groups III and IV, they still remained higher than those of the control group. Conclusion: Exposure to hypobaric hypoxia leads to an increase in MDA and carbonyl levels in the skeletal muscles, indicating an elevation of oxidative stress levels. However, the subsequent intermittent hypobaric hypoxia exposure resulted in a reduction in these levels, implying that skeletal muscles may adapt to hypoxic conditions.
{"title":"Malondialdehyde and carbonyl levels in skeletal muscle tissues after intermittent hypobaric hypoxia exposures","authors":"S. Dewi, Alexander Rafael Satyadharma, A. R. Danendra, Wardaya","doi":"10.32889/actabioina.113","DOIUrl":"https://doi.org/10.32889/actabioina.113","url":null,"abstract":"Background: Hypobaric hypoxia is a state of decreased oxygen pressure at high altitudes that can lead to hypoxia and oxidative stress as a result. Skeletal muscle is one of the important organs that can be affected by oxidative stress and cause contractile dysfunction. \u0000Objective: This study aimed to evaluate the impact of intermittent hypobaric hypoxia on oxidative stress markers in rat skeletal muscle, by measuring malondialdehyde (MDA) and carbonyl levels. \u0000Methods: Twenty-five Wistar rats were allocated into five groups, including one control group and four hypoxic groups (I-IV). The hypoxic groups were exposed to an altitude of 25,000 feet for 5 minutes using hypobaric chamber in once (I), twice (II), three (III), and four (IV) times, with a 7-day interval period between exposures. The control group remained in normobaric conditions throughout the study. MDA levels were measured by thiobarbituric acid (TBA) test, while carbonyl levels were measured using 2,4-dinitrophenylhydrazine (DNPH) reagent. \u0000Results: The MDA level was significantly increased in group I compared to the control group (p=0.008). There were decreasing MDA levels in groups II, III, and IV compared to group I. The carbonyl level was significantly higher in group I than the control group (p=0.000), with an even higher level observed in group II. Although the carbonyl levels tended to decrease in groups III and IV, they still remained higher than those of the control group. \u0000Conclusion: Exposure to hypobaric hypoxia leads to an increase in MDA and carbonyl levels in the skeletal muscles, indicating an elevation of oxidative stress levels. However, the subsequent intermittent hypobaric hypoxia exposure resulted in a reduction in these levels, implying that skeletal muscles may adapt to hypoxic conditions.","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"88 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132684337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kenny Cantika Abadi, Febriana Catur Iswanti, S. A. Jusman, Fadilah Fadilah, A. R. Prijanti
Background: Maternal exposure to cigarette smoke during pregnancy and lactation might harmful for the fetus. The smoke contains many free radicals that could be eliminated by antioxidant. This study aimed to investigate the effect of Spirulina platensis ethanol extract as antioxidant against cigarette smoke exposure during pregnancy until lactation by assessing oxidative stress markers in neonatal brain tissues. Methods: The experimental study used 26 offspring divided into four groups: (C) = offspring of maternal control group; (Cg) = offspring of maternal exposed to cigarette smoke; (CgSp) = offspring of maternal given spirulina and exposed to cigarette smoke; and (Sp) = offspring of maternal given spirulina only group, during gestation and 9 days lactation (30 days). Each group consisted of 6 offspring obtained from 2 adult females mated with male Sprague-Dawley rats. The exposure of cigarette smoke was 4 burn cigarettes/day for 30 days. The dose of extract was 200 mg/kg BW. The offspring were sacrificed, and the brain tissues were taken for MDA, MnSOD activity, as well as catalase activity, carbonyl, and GSH. Results: There was no significant differences in MDA level between groups. The carbonyl, SOD, and catalase activity did not differ between the control and smoked group. Conclusion: Exposure of four burned cigarettes smoke per day during pregnancy, and 9 days of lactation did not trigger oxidative stress. However, the effect of Spirulina platensis administration on rat offspring brain could not be analyzed.
{"title":"Spirulina platensis effect on oxidative stress of rat’s offspring brain exposed to cigarette smoke during pregnancy and lactation","authors":"Kenny Cantika Abadi, Febriana Catur Iswanti, S. A. Jusman, Fadilah Fadilah, A. R. Prijanti","doi":"10.32889/actabioina.64","DOIUrl":"https://doi.org/10.32889/actabioina.64","url":null,"abstract":"Background: Maternal exposure to cigarette smoke during pregnancy and lactation might harmful for the fetus. The smoke contains many free radicals that could be eliminated by antioxidant. This study aimed to investigate the effect of Spirulina platensis ethanol extract as antioxidant against cigarette smoke exposure during pregnancy until lactation by assessing oxidative stress markers in neonatal brain tissues. \u0000Methods: The experimental study used 26 offspring divided into four groups: (C) = offspring of maternal control group; (Cg) = offspring of maternal exposed to cigarette smoke; (CgSp) = offspring of maternal given spirulina and exposed to cigarette smoke; and (Sp) = offspring of maternal given spirulina only group, during gestation and 9 days lactation (30 days). Each group consisted of 6 offspring obtained from 2 adult females mated with male Sprague-Dawley rats. The exposure of cigarette smoke was 4 burn cigarettes/day for 30 days. The dose of extract was 200 mg/kg BW. The offspring were sacrificed, and the brain tissues were taken for MDA, MnSOD activity, as well as catalase activity, carbonyl, and GSH. \u0000Results: There was no significant differences in MDA level between groups. The carbonyl, SOD, and catalase activity did not differ between the control and smoked group. \u0000Conclusion: Exposure of four burned cigarettes smoke per day during pregnancy, and 9 days of lactation did not trigger oxidative stress. However, the effect of Spirulina platensis administration on rat offspring brain could not be analyzed.","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123498012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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