N. Mudjihartini, D. E. Andayani, Sheira Taflah Putri Handana
Background: Human milk contains many components, one of them is superoxide dismutase (SOD). Vitamin E and C, together with SOD, can prevent oxidative stress. Objective: This study investigated the correlation between vitamin E and vitamin C intake, with total SOD activity, in erythrocyte and breast milk among lactating mothers in Jakarta, Indonesia. Methods: Sixty lactating mothers aged 20–40 years were recruited in 1–6 months postpartum in Grogol Petamburan and the Cilincing Public Health Centre from March 2019 until April 2019. Vitamins E and C dietary intake were collected using a semi-quantitative food frequency questionnaire. SOD total activity of erythrocyte and breast milk was measured using the Ransod kit 125. Results: The median value of vitamin E intake was 6.50 mg/day, showing 91.7% of patients do not meet recommended daily intake (RDA) (19 gram/day), and the median of vitamin C intake was 120.05 mg/day with 70% participants fulfilling RDA. SOD total activity in erythrocyte and breastmilk showed a median value of 423.73 U/mL and 58.34 U/mL, respectively. The correlation between vitamin E intake with total SOD activity in erythrocyte (r = 0.143 p > 0,05) and breast milk (r = 0.041, p > 0,05) was not significant. Vitamin C intake was also not significantly correlated with SOD total activity in the erythrocyte. Conclusion: There is no significant correlation between vitamin E and vitamin C intake with the total activity of SOD of erythrocyte and breast milk in lactating mothers.
背景:母乳中含有多种成分，其中超氧化物歧化酶(SOD)就是其中之一。维生素E和C与SOD一起可以防止氧化应激。目的:研究印度尼西亚雅加达哺乳期母亲红细胞和母乳中维生素E和维生素C摄入量与总SOD活性的关系。方法:于2019年3月至2019年4月在格罗戈尔-佩坦布兰和希林林公共卫生中心招募60名年龄在20-40岁的产后1-6个月的哺乳期母亲。采用半定量食物频率问卷收集膳食中维生素E和C的摄入量。采用Ransod试剂盒125检测红细胞和母乳中SOD总活性。结果:维生素E摄入量中位数为6.50 mg/d, 91.7%的患者未达到推荐日摄入量(19 g/d)，维生素C摄入量中位数为120.05 mg/d, 70%的患者达到推荐日摄入量。红细胞和母乳中SOD总活性的中位数分别为423.73 U/mL和58.34 U/mL。维生素E摄入量与红细胞总SOD活性(r = 0.143 p > 0.05)和母乳(r = 0.041, p > 0.05)的相关性不显著。维生素C摄入量与红细胞SOD总活性也无显著相关。结论:哺乳期母亲维生素E和维生素C摄入量与红细胞和母乳中SOD总活性无显著相关性。
{"title":"The relationship between vitamin E and C intake with total activity of erythrocytes and breast milk superoxide dismutase in lactating mothers","authors":"N. Mudjihartini, D. E. Andayani, Sheira Taflah Putri Handana","doi":"10.32889/actabioina.15","DOIUrl":"https://doi.org/10.32889/actabioina.15","url":null,"abstract":"Background: Human milk contains many components, one of them is superoxide dismutase (SOD). Vitamin E and C, together with SOD, can prevent oxidative stress. \u0000Objective: This study investigated the correlation between vitamin E and vitamin C intake, with total SOD activity, in erythrocyte and breast milk among lactating mothers in Jakarta, Indonesia. \u0000Methods: Sixty lactating mothers aged 20–40 years were recruited in 1–6 months postpartum in Grogol Petamburan and the Cilincing Public Health Centre from March 2019 until April 2019. Vitamins E and C dietary intake were collected using a semi-quantitative food frequency questionnaire. SOD total activity of erythrocyte and breast milk was measured using the Ransod kit 125. \u0000Results: The median value of vitamin E intake was 6.50 mg/day, showing 91.7% of patients do not meet recommended daily intake (RDA) (19 gram/day), and the median of vitamin C intake was 120.05 mg/day with 70% participants fulfilling RDA. SOD total activity in erythrocyte and breastmilk showed a median value of 423.73 U/mL and 58.34 U/mL, respectively. The correlation between vitamin E intake with total SOD activity in erythrocyte (r = 0.143 p > 0,05) and breast milk (r = 0.041, p > 0,05) was not significant. Vitamin C intake was also not significantly correlated with SOD total activity in the erythrocyte. \u0000Conclusion: There is no significant correlation between vitamin E and vitamin C intake with the total activity of SOD of erythrocyte and breast milk in lactating mothers.","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"119 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132335929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abdul Halim Sadikin, Irene Dian, Mukharjon Mukharjon, Rini Puspitaningtum, S. Wanandi
Background: In some people, acetaldehyde, a toxic product from ethanol oxidation, cannot be oxidized to acetate. The excess of acetaldehyde could cause facial flushing, dizziness, and hypertension when they consume ethanol. This ethanol sensitivity is caused by a deficiency of ALDH2. Objective: This study aims to analyze and count the polymorphism frequency of the ALDH2 gene in Indonesia’s Minang ethnic. Methods: DNA samples were taken randomly from hair bulbous of 60 subjects (male and female, 3rd generation). A nested polymerase chain reaction was conducted to amplify the ALDH2 in the samples. Afterward, restriction fragment length polymorphism (RFLP) was conducted to the amplicons using the EcoRI restriction enzyme. The measured parameters were the distribution of the wildtype, atypical homozygote, and heterozygote. Results: Results showed that out of 60 subjects, 53.33% have an atypical homozygote gene (subjects prone to hypersensitive to alcohol), 28.33% have a heterozygote gene, and 18.33% have a wildtype gene. The frequency of the atypical alleles in Minang ethnic is 0.675. Conclusion: The atypical ALDH2 allele was much higher than the normal ALDH2 allele, in which most participants have atypical homozygote ALDH2, suggesting the samples are sensitive to alcohol.
{"title":"Genetic polymorphism of ALDH2 in Indonesia’s Minang ethnic","authors":"Abdul Halim Sadikin, Irene Dian, Mukharjon Mukharjon, Rini Puspitaningtum, S. Wanandi","doi":"10.32889/actabioina.14","DOIUrl":"https://doi.org/10.32889/actabioina.14","url":null,"abstract":"Background: In some people, acetaldehyde, a toxic product from ethanol oxidation, cannot be oxidized to acetate. The excess of acetaldehyde could cause facial flushing, dizziness, and hypertension when they consume ethanol. This ethanol sensitivity is caused by a deficiency of ALDH2. \u0000Objective: This study aims to analyze and count the polymorphism frequency of the ALDH2 gene in Indonesia’s Minang ethnic. \u0000Methods: DNA samples were taken randomly from hair bulbous of 60 subjects (male and female, 3rd generation). A nested polymerase chain reaction was conducted to amplify the ALDH2 in the samples. Afterward, restriction fragment length polymorphism (RFLP) was conducted to the amplicons using the EcoRI restriction enzyme. The measured parameters were the distribution of the wildtype, atypical homozygote, and heterozygote. \u0000Results: Results showed that out of 60 subjects, 53.33% have an atypical homozygote gene (subjects prone to hypersensitive to alcohol), 28.33% have a heterozygote gene, and 18.33% have a wildtype gene. The frequency of the atypical alleles in Minang ethnic is 0.675. \u0000Conclusion: The atypical ALDH2 allele was much higher than the normal ALDH2 allele, in which most participants have atypical homozygote ALDH2, suggesting the samples are sensitive to alcohol.","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114826374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Hamka, Fatimawali Fatimawali, O. Datu, T. Tallei
Background: Betel (Piper betle L.) is a well-known medicinal plant for its numerous health benefits. Saponins, flavonoids, polyphenols, and essential oils are among the chemical constituents of betel plants. Flavonoids are one of the most common groups of secondary metabolites found in plant tissues, including in betel plants. Objective: The purpose of this research is to isolate flavonoids from betel fruit and to determine the antibacterial activity of betel fruit extract against Pseudomonas aeruginosa. Methods: Thin-layer chromatography (TLC) with the eluent chloroform: methanol: water was used to isolate flavonoids. UV-Vis spectrophotometry was used to determine the presence of flavonoids in betel fruit. The antibacterial activity of extract and TLC-isolates of betel fruit was tested by using the disc method. Results: TLC analysis resulted in the formation of a brown stain. The UV-Vis spectrophotometry results revealed two absorption bands at 366 nm and 268 nm, indicating that flavonoids are present in betel fruit. Antibacterial activity test against Pseudomonas aeruginosa bacteria showed that the concentration of 30% and 60% of betel fruit extract had strong antibacterial activity. Conclusion: The results revealed that the betel fruit contains flavonoid compounds, and the extract has medium to strong antibacterial activity.
{"title":"Antibacterial activity of betel (Piper betle L.) fruit against Pseudomonas aeruginosa","authors":"A. Hamka, Fatimawali Fatimawali, O. Datu, T. Tallei","doi":"10.32889/actabioina.10","DOIUrl":"https://doi.org/10.32889/actabioina.10","url":null,"abstract":"Background: Betel (Piper betle L.) is a well-known medicinal plant for its numerous health benefits. Saponins, flavonoids, polyphenols, and essential oils are among the chemical constituents of betel plants. Flavonoids are one of the most common groups of secondary metabolites found in plant tissues, including in betel plants. \u0000Objective: The purpose of this research is to isolate flavonoids from betel fruit and to determine the antibacterial activity of betel fruit extract against Pseudomonas aeruginosa. \u0000Methods: Thin-layer chromatography (TLC) with the eluent chloroform: methanol: water was used to isolate flavonoids. UV-Vis spectrophotometry was used to determine the presence of flavonoids in betel fruit. The antibacterial activity of extract and TLC-isolates of betel fruit was tested by using the disc method. \u0000Results: TLC analysis resulted in the formation of a brown stain. The UV-Vis spectrophotometry results revealed two absorption bands at 366 nm and 268 nm, indicating that flavonoids are present in betel fruit. Antibacterial activity test against Pseudomonas aeruginosa bacteria showed that the concentration of 30% and 60% of betel fruit extract had strong antibacterial activity. \u0000Conclusion: The results revealed that the betel fruit contains flavonoid compounds, and the extract has medium to strong antibacterial activity.","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123020695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: The pathophysiological mechanism associated with spontaneous preterm delivery is oxidative stress through the increased formation of reactive oxygen species (ROS) due to lipid peroxidation. Malondialdehyde (MDA) is one of the biomarkers of oxidative stress produced through the lipid peroxidation process. Objective: The aim of this study is to observe the difference in MDA levels among preterm labor compared to full-term labor. Methods: Observational research was conducted with a comparative cross-sectional design. Maternal venous blood samples were taken from private hospitals and midwives in Padang city and Aro Suka Hospital Solok Regency. Samples were selected by consecutive sampling and divided into two groups with a total of 40 samples. MDA level was measured using the spectrophotometry method. Results: MDA levels in preterm delivery were 3,6±0.42 nmol/mL and in full-term delivery were 2.9±0.33 nmol/mL. Conclusion: There was a significant difference in MDA levels between preterm labor and full-term delivery. MDA levels in preterm childbirth were higher than MDA levels in full-term delivery.
{"title":"Increased level of malondialdehyde in preterm labor","authors":"Yesi Mustika Sari, Eti Yerizel","doi":"10.32889/actabioina.8","DOIUrl":"https://doi.org/10.32889/actabioina.8","url":null,"abstract":"Background: The pathophysiological mechanism associated with spontaneous preterm delivery is oxidative stress through the increased formation of reactive oxygen species (ROS) due to lipid peroxidation. Malondialdehyde (MDA) is one of the biomarkers of oxidative stress produced through the lipid peroxidation process. \u0000Objective: The aim of this study is to observe the difference in MDA levels among preterm labor compared to full-term labor. \u0000Methods: Observational research was conducted with a comparative cross-sectional design. Maternal venous blood samples were taken from private hospitals and midwives in Padang city and Aro Suka Hospital Solok Regency. Samples were selected by consecutive sampling and divided into two groups with a total of 40 samples. MDA level was measured using the spectrophotometry method. \u0000Results: MDA levels in preterm delivery were 3,6±0.42 nmol/mL and in full-term delivery were 2.9±0.33 nmol/mL. \u0000Conclusion: There was a significant difference in MDA levels between preterm labor and full-term delivery. MDA levels in preterm childbirth were higher than MDA levels in full-term delivery.","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114387436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Increasing blood sugar level may increase free radical compounds in type 2 diabetes. Free radical compounds can cause oxidative stress, thereby decreasing endogenous antioxidants such as reduced glutathione (GSH). Objective: This study aimed to determine whether random blood glucose levels affect GSH in type 2 diabetes patients within the Malay race. Methods: This study was observational with case-control, involving 25 patients with uncomplicated type 2 diabetes (receiving metformin and/or glimipiride) and 25 healthy controls. Random blood glucose levels were determined using ACCU-CHECK® Kit. Blood GSH levels were determined by Sigma GSH Assay Kit. Results: Results show that type 2 diabetes patients have a significantly lower random blood glucose level compared with those of age-matched normal subjects (p<0.0001). Type 2 diabetic patients had significantly lower levels of GSH (p=0.00) than those of age-matched normal subjects. We found a moderate negative correlation (r=-0.437 and p=0.02) between the level of random blood glucose and the level of GSH. Conclusion: The depletion of GSH during hyperglycemia may neutralize the free radicals indirectly generated by the abundant of glucose.
{"title":"Effect of glucose on reduced glutathione level in Malay uncomplicated type 2 diabetes patients","authors":"Subandrate, Raafqi Ranasasmita","doi":"10.32889/actabioina.13","DOIUrl":"https://doi.org/10.32889/actabioina.13","url":null,"abstract":"Background: Increasing blood sugar level may increase free radical compounds in type 2 diabetes. Free radical compounds can cause oxidative stress, thereby decreasing endogenous antioxidants such as reduced glutathione (GSH). \u0000Objective: This study aimed to determine whether random blood glucose levels affect GSH in type 2 diabetes patients within the Malay race. \u0000Methods: This study was observational with case-control, involving 25 patients with uncomplicated type 2 diabetes (receiving metformin and/or glimipiride) and 25 healthy controls. Random blood glucose levels were determined using ACCU-CHECK® Kit. Blood GSH levels were determined by Sigma GSH Assay Kit. \u0000Results: Results show that type 2 diabetes patients have a significantly lower random blood glucose level compared with those of age-matched normal subjects (p<0.0001). Type 2 diabetic patients had significantly lower levels of GSH (p=0.00) than those of age-matched normal subjects. We found a moderate negative correlation (r=-0.437 and p=0.02) between the level of random blood glucose and the level of GSH. \u0000Conclusion: The depletion of GSH during hyperglycemia may neutralize the free radicals indirectly generated by the abundant of glucose. \u0000 ","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126338770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-02-01DOI: 10.32889/ACTABIOINA.V3I2.51
Angga Crystal Loasana Yami, I. Batubara, K. A. Audah
Background : The treatment of some diseases caused by free radicals and pathogenic bacteria usually by using antioxidants and antibiotics. Due to excessive use of antibiotics and other environmental cues, some bacteria are now resistant to certain antibiotics or even to multiple antibiotics. Some Vibrio cholerae bacterial strains are multiresistant to many antibiotics.Objective : The antioxidant and antibacterial activities of Brugueira gymnorrhiza stem extracts against pathogenic bacteria V. cholerae.Method : The B. gymnorrhiza stem was extracted by gradient maceration method. The DPPH method was used to determine the antioxidant activity and the disc diffusion method was used to determine the antibacterial activities. The column chromatography method was used to fractionate the selective extract with the best activity. The LC-MS/MS method was used to identify the compound obtained from the fraction with the best antioxidant and antibacterial activity.Result : Ethyl acetate extract of B. gymnorrhiza stem had the best antibacterial activity with MIC and MBC values of 62.50 mg/L. Ethyl acetate extract also showed the best value of antioxidant activity as indicated by an IC50 value of 255.03 mg/L. The results of fractions test showed that fraction 3 had the best antibacterial and the best antioxidant activities with both the MIC and MBC values of 7.90 mg/L and IC50 value of 348.91 mg/L, respectively.Conclusion : Ethyl acetate extract of B. gymnorrhiza stem has good potential as antioxidant and antibacterial. The compound which is thought as antioxidant and antibacterial from Ethyl acetate extract is 2-Ethyl-4-methyl-1H-imidazole.
{"title":"ANTIOXIDANT AND ANTIBACTERIAL ACTIVITY OF MANGROVE Brugueira gymnorrhiza STEM EXTRACTS AGAINST PATHOGENIC BACTERIA Vibrio cholerae","authors":"Angga Crystal Loasana Yami, I. Batubara, K. A. Audah","doi":"10.32889/ACTABIOINA.V3I2.51","DOIUrl":"https://doi.org/10.32889/ACTABIOINA.V3I2.51","url":null,"abstract":"Background : The treatment of some diseases caused by free radicals and pathogenic bacteria usually by using antioxidants and antibiotics. Due to excessive use of antibiotics and other environmental cues, some bacteria are now resistant to certain antibiotics or even to multiple antibiotics. Some Vibrio cholerae bacterial strains are multiresistant to many antibiotics.Objective : The antioxidant and antibacterial activities of Brugueira gymnorrhiza stem extracts against pathogenic bacteria V. cholerae.Method : The B. gymnorrhiza stem was extracted by gradient maceration method. The DPPH method was used to determine the antioxidant activity and the disc diffusion method was used to determine the antibacterial activities. The column chromatography method was used to fractionate the selective extract with the best activity. The LC-MS/MS method was used to identify the compound obtained from the fraction with the best antioxidant and antibacterial activity.Result : Ethyl acetate extract of B. gymnorrhiza stem had the best antibacterial activity with MIC and MBC values of 62.50 mg/L. Ethyl acetate extract also showed the best value of antioxidant activity as indicated by an IC50 value of 255.03 mg/L. The results of fractions test showed that fraction 3 had the best antibacterial and the best antioxidant activities with both the MIC and MBC values of 7.90 mg/L and IC50 value of 348.91 mg/L, respectively.Conclusion : Ethyl acetate extract of B. gymnorrhiza stem has good potential as antioxidant and antibacterial. The compound which is thought as antioxidant and antibacterial from Ethyl acetate extract is 2-Ethyl-4-methyl-1H-imidazole.","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"201 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114752183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-29DOI: 10.32889/actabioina.v3i1.61
KA Audah
[No abstract available]
[没有摘要]
{"title":"HOW TO OVERCOME THE FEAR OF CORONAVIRUS?","authors":"KA Audah","doi":"10.32889/actabioina.v3i1.61","DOIUrl":"https://doi.org/10.32889/actabioina.v3i1.61","url":null,"abstract":"[No abstract available]","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125176203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-29DOI: 10.32889/actabioina.v3i1.29
N. A. Yudhaswara, A. Prijanti, M. Sadikin
Background: Lipoic acid is a substance contained in intra and extracellular that act as a coenzyme of Pyruvate Dehydrogenase, also as an antidote, chelating agent and antioxidant. Measurement of lipoic acid is needed to determine the amount of lipoic acid that performs its functions either as a coenzyme or an antioxidant. Besides, this measurement requires a special tool such as High Performance Liquid Chromatography (HPLC) and a process that is available in rural or simple laboratories. Objective: A common and easy tool such as a spectrophotometer was conducted and could expected to be a tool of lipoic acid determination in body fluid such as plasma.Methods: Measurement of lipoic acid using spectrophotometry with UV methanol and visible PdCl2 has been tested and compared to HPLC measurement that was valid and reliable in drug measurement or pharmaceutical preparations.Results: Determination of lipoic acid in plasma and leukocytes using PdCl2 produced replicable, reliability and valid result, with high accuracy, precision and was not different from lipoic acid measurement using HPLC, p=0.99. While UV methanol was different compare to HPLC p =0.0001 or was not valid.Conclusion: The measurement of lipoic acid using PdCl2 visible method can be applied to determine the levels of lipoic acid (LA) and DHLA in plasma and equal to HPLC result.
{"title":"PALLADIUM (II) CHLORIDE (PdCl2) SPECTROPHOTOMETRY TO DETERMINE LIPOIC ACID CONCENTRATION IN PLASMA AND LEUKOCYTES","authors":"N. A. Yudhaswara, A. Prijanti, M. Sadikin","doi":"10.32889/actabioina.v3i1.29","DOIUrl":"https://doi.org/10.32889/actabioina.v3i1.29","url":null,"abstract":"Background: Lipoic acid is a substance contained in intra and extracellular that act as a coenzyme of Pyruvate Dehydrogenase, also as an antidote, chelating agent and antioxidant. Measurement of lipoic acid is needed to determine the amount of lipoic acid that performs its functions either as a coenzyme or an antioxidant. Besides, this measurement requires a special tool such as High Performance Liquid Chromatography (HPLC) and a process that is available in rural or simple laboratories. Objective: A common and easy tool such as a spectrophotometer was conducted and could expected to be a tool of lipoic acid determination in body fluid such as plasma.Methods: Measurement of lipoic acid using spectrophotometry with UV methanol and visible PdCl2 has been tested and compared to HPLC measurement that was valid and reliable in drug measurement or pharmaceutical preparations.Results: Determination of lipoic acid in plasma and leukocytes using PdCl2 produced replicable, reliability and valid result, with high accuracy, precision and was not different from lipoic acid measurement using HPLC, p=0.99. While UV methanol was different compare to HPLC p =0.0001 or was not valid.Conclusion: The measurement of lipoic acid using PdCl2 visible method can be applied to determine the levels of lipoic acid (LA) and DHLA in plasma and equal to HPLC result.","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129221023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-29DOI: 10.32889/actabioina.v3i1.47
Pocut Astari, Y. Siregar, Ariyati Yosi
Background: Psoriasis is a chronic inflammation of the skin caused by combination of genetic, immune and environmental factors. The Vitamin D receptors alongside with plasma vitamin D (25(OH)D) level have known to be related with psoriasis. Vitamin D receptor polymorphism is one of the multiple polymorphisms that predispose individuals to several diseases. It is possible that this polymorphism is different among psoriatic patients and healthy one particularly in Medan, Indonesia population. Objective: We aimed to investigate associations between plasma level of 25(OH)D and one VDR gene polymorphism (A-1012G) with psoriasis.Methods: Fourty four psoriatic patients and 44 healthy control subjects’ DNA samples were obtained in this case control study and genotyped for A-1012G polymorphism by Polymerase Chain Reaction (PCR). The plasma vitamin D (25(OH)D) level of case and control subjects were examined using Enzyme Linked Immunosorbent Assay (ELISA).Results: Significant lower plasma 25(OH)D levels were found in control group (p<0.001) which consist of mostly young adult female. There is no significant relationship between AA, AG and GG genotype variances of A-1012G polymorphism with psoriasis (p=0.124).Conclusion: No significant association found between A-1012G polymorphism and psoriasis but there was a significant difference found between vitamin D level and psoriasis (p<0.001). From this study, vitamin D deficiency were more common in young adult female
{"title":"ASSOCIATION OF VITAMIN D LEVEL AND VITAMIN D RECEPTOR A-1012G POLYMORPHISM WITH PSORIASIS – CASE CONTROL STUDY","authors":"Pocut Astari, Y. Siregar, Ariyati Yosi","doi":"10.32889/actabioina.v3i1.47","DOIUrl":"https://doi.org/10.32889/actabioina.v3i1.47","url":null,"abstract":"Background: Psoriasis is a chronic inflammation of the skin caused by combination of genetic, immune and environmental factors. The Vitamin D receptors alongside with plasma vitamin D (25(OH)D) level have known to be related with psoriasis. Vitamin D receptor polymorphism is one of the multiple polymorphisms that predispose individuals to several diseases. It is possible that this polymorphism is different among psoriatic patients and healthy one particularly in Medan, Indonesia population. Objective: We aimed to investigate associations between plasma level of 25(OH)D and one VDR gene polymorphism (A-1012G) with psoriasis.Methods: Fourty four psoriatic patients and 44 healthy control subjects’ DNA samples were obtained in this case control study and genotyped for A-1012G polymorphism by Polymerase Chain Reaction (PCR). The plasma vitamin D (25(OH)D) level of case and control subjects were examined using Enzyme Linked Immunosorbent Assay (ELISA).Results: Significant lower plasma 25(OH)D levels were found in control group (p<0.001) which consist of mostly young adult female. There is no significant relationship between AA, AG and GG genotype variances of A-1012G polymorphism with psoriasis (p=0.124).Conclusion: No significant association found between A-1012G polymorphism and psoriasis but there was a significant difference found between vitamin D level and psoriasis (p<0.001). From this study, vitamin D deficiency were more common in young adult female","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130935849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-12-29DOI: 10.32889/actabioina.v3i1.50
MF Adhiwirawan, Y. Nugraha, C. Fauziah
Background: The usage of Cyclophosphamide (Cp) leads to infertility of reproductive system caused by acrolein. Acrolein can itself cause oxidative damage by depletion of cellular glutathione (GSH) by conjugation, leading to membrane disruption, DNA and mitochondrial damage and can exacerbate apoptosis, which may affect spermatogenesis. Zinc (Zn) which is constituents of superoxide dismutase, has a protective effect towards free radicals from physiological or pathologic effects to minimize the cell’s damage. Objective: The purpose of this research was to know the effect of Zn on the spermatozoa count of Mus musculus that given Cyclophosphamide intraperitoneally (ip).Methods: In the present study, Cyclophosphamide was administered in saline 200 mg/kg 1x weekly for 5 weeks by ip route, whereas Zn was supplemented by oral route with doses of 25, 50, 100 mg/Kg/day for 5 weeks. The data were analyzed with Anova and followed by Bonferroni Test at a significant level of 5%.Results: The result of this research revealed that high Zn diet and Cp administration decrease sperm count simultaneously. It showed by the decrease of sperm count from 1490 (1 x 103)/ml sperm in control group becomes 240 (1 x 103)/ml sperm in treatment group with 100 mg/Kg of oral Zn and 200 mg/kg of CpConclusion: This research show that Zn supplement to prevent Cyclophosphamide toxic effect in spermatogenesis doesn’t have a protective effect, in fact its reduce sperm count by excess of Methallothine production and alter the spermatogenesis by reduce Cu intake from intestine.
背景:使用环磷酰胺(Cp)可导致丙烯醛引起的生殖系统不孕症。丙烯醛本身可通过偶联作用使细胞谷胱甘肽(GSH)耗竭而引起氧化损伤，导致膜破坏、DNA和线粒体损伤，并可加剧细胞凋亡，从而影响精子发生。锌(Zn)是超氧化物歧化酶的组成成分，对自由基具有保护作用，使细胞免受生理或病理作用的损害。目的:了解锌对腹腔注射环磷酰胺的小家鼠精子数量的影响。方法:口服环磷酰胺200 mg/kg生理盐水1次，每周1次，连续5周;口服锌25、50、100 mg/kg /天，连续5周。数据采用方差分析和Bonferroni检验，显著水平为5%。结果:高锌饮食和加Cp可同时降低精子数量。它显示的精子数减少1490 (1 x 103) /毫升精液对照组成为240 (1 x 103) /毫升精液治疗组与100毫克/公斤口服锌和200毫克/公斤CpConclusion:这项研究表明,锌补充剂预防环磷酰胺毒性作用在精子发生没有保护作用,事实上其精子数减少Methallothine生产过剩和改变精子形成来自小肠的减少铜的摄入。
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