Pub Date : 2019-09-21DOI: 10.32889/actabioina.v2i1.35
Endang Maya Sari, Riryn Novianty, A. Awaluddin, S. Saryono, N. Pratiwi
Background: Biodegradation of petroleum hydrocarbon needs a specific technique called bioremediation to remove the environmental pollutants. Several indigenous microorganisms including fungi, bacteria, and actinomycetes are effective agents in degrading petroleum derivatives, aliphatic and polyaromatic hydrocarbons (PAHs).Objective: This research aimed to investigate indigenous fungi isolates from petroleum hydrocarbon contaminated soil in Siak which are capable to degrade hydrocarbon.Methods: The competence of indigenous fungi was isolated from a crude oil-contaminated soil which collected from one of oil-field in Siak, Riau. The effectiveness of isolates on the degradation crude oil was tested by culturing the isolates in Bushnell-Haas broth containing crude oil (5% v/v) for 16 days. A decrease in pH, change in optical density and amount of CO2 released were recorded to indirectly indicate the crude oil degradation by the fungi. To measure the percentage of crude oil biodegradation, gravimetric analysis was utilized.Results: The two colonies were selected and identified as Aspergillus sp LBKURCC151 and Penicillium sp LBKURCC153. The results showed that Aspergillus sp LBKURCC151 reached a higher level (61%) of biodegradation after 16 days under the optimum conditions in degrading total petroleum hydrocarbon than Penicillium sp LBKURCC153 (46%).Conclusion: These results indicated that Aspergillus sp LBKURCC151 and Penicillium sp LBKURCC153 are potential degraders for bioremediation in crude oil-contaminated area.
{"title":"EFFECTIVENESS OF CRUDE OIL DEGRADING FUNGI ISOLATED FROM PETROLEUM HYDROCARBON CONTAMINATED SOIL IN SIAK, RIAU","authors":"Endang Maya Sari, Riryn Novianty, A. Awaluddin, S. Saryono, N. Pratiwi","doi":"10.32889/actabioina.v2i1.35","DOIUrl":"https://doi.org/10.32889/actabioina.v2i1.35","url":null,"abstract":"Background: Biodegradation of petroleum hydrocarbon needs a specific technique called bioremediation to remove the environmental pollutants. Several indigenous microorganisms including fungi, bacteria, and actinomycetes are effective agents in degrading petroleum derivatives, aliphatic and polyaromatic hydrocarbons (PAHs).Objective: This research aimed to investigate indigenous fungi isolates from petroleum hydrocarbon contaminated soil in Siak which are capable to degrade hydrocarbon.Methods: The competence of indigenous fungi was isolated from a crude oil-contaminated soil which collected from one of oil-field in Siak, Riau. The effectiveness of isolates on the degradation crude oil was tested by culturing the isolates in Bushnell-Haas broth containing crude oil (5% v/v) for 16 days. A decrease in pH, change in optical density and amount of CO2 released were recorded to indirectly indicate the crude oil degradation by the fungi. To measure the percentage of crude oil biodegradation, gravimetric analysis was utilized.Results: The two colonies were selected and identified as Aspergillus sp LBKURCC151 and Penicillium sp LBKURCC153. The results showed that Aspergillus sp LBKURCC151 reached a higher level (61%) of biodegradation after 16 days under the optimum conditions in degrading total petroleum hydrocarbon than Penicillium sp LBKURCC153 (46%).Conclusion: These results indicated that Aspergillus sp LBKURCC151 and Penicillium sp LBKURCC153 are potential degraders for bioremediation in crude oil-contaminated area.","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126995275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-21DOI: 10.32889/actabioina.v2i1.33
Abdul Halim Sadikin, Y. Moenadjat, Novi Sylvia Hardiany
Background: Usually it takes a large number of volume sample to determine blood group from external secretion fluids. But, in certain condition, samples are only available in very small amount. The objective of this study is to detect the presence of ABO blood group substances in mucosal fluid using ELISA technique, thus only requires small amount of samples.Objective: To develop an ELISA technique using the current anti-ABO antibodies for determination of blood group by hemagglutination technique and second peroxidase label antibody specific for mouse IgG, originally used for another ELISA technique.Methods: 100 μl of diluted human intestinal mucosal fluid were incubated overnight in 4oC in ELISA microplate wells, followed by addition anti-ABO antibodies. Then after incubation, a second revealing antibody anti mouse IgG labeled with peroxidase was added. After a brief incubation, substrate H2O2 and chromogenic TMB were added.Results: Positive reaction is marked by development of blue colour, which, on termination enzymatic reaction by addition 100 μl H2SO4 change to yellow.Conclusion: An ELISA method for detecting ABO substance in mucosal fluid can be developed from antibodies not specifically made for this technique, but specific only for the target.
{"title":"ELISA METHOD TO DETECT ABO BLOOD GROUP IN EXTERNAL SECRETION FLUIDS","authors":"Abdul Halim Sadikin, Y. Moenadjat, Novi Sylvia Hardiany","doi":"10.32889/actabioina.v2i1.33","DOIUrl":"https://doi.org/10.32889/actabioina.v2i1.33","url":null,"abstract":"Background: Usually it takes a large number of volume sample to determine blood group from external secretion fluids. But, in certain condition, samples are only available in very small amount. The objective of this study is to detect the presence of ABO blood group substances in mucosal fluid using ELISA technique, thus only requires small amount of samples.Objective: To develop an ELISA technique using the current anti-ABO antibodies for determination of blood group by hemagglutination technique and second peroxidase label antibody specific for mouse IgG, originally used for another ELISA technique.Methods: 100 μl of diluted human intestinal mucosal fluid were incubated overnight in 4oC in ELISA microplate wells, followed by addition anti-ABO antibodies. Then after incubation, a second revealing antibody anti mouse IgG labeled with peroxidase was added. After a brief incubation, substrate H2O2 and chromogenic TMB were added.Results: Positive reaction is marked by development of blue colour, which, on termination enzymatic reaction by addition 100 μl H2SO4 change to yellow.Conclusion: An ELISA method for detecting ABO substance in mucosal fluid can be developed from antibodies not specifically made for this technique, but specific only for the target.","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"158 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126208665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-21DOI: 10.32889/actabioina.v2i1.31
Maria Christina Dwiyanti, R. Benettan, F. Wandy, M. Lirendra, F. Ferdinal, D. Limanan
Background: Hyperoxia is a state of oversupply of oxygen in tissues and organs that can increase reactive oxygen species (ROS). When antioxidants cannot balance ROS levels, oxidative stress occurs. Catalase and reduced glutathione (GSH) are two of the antioxidants that can be very useful to counteract ROS. Increased production of ROS subsequently results in lipids damage and generates malondialdehyde (MDA). ROS interaction with cardiac cells causes remodeling thus leads to heart failure.Objectives: The purpose of this study was to find out the changes on oxidative stress-related biomarkers in plasma and cardiac tissue. Methods: Sprague Dawley rats were divided into 5 groups (n=6/group). Control group was exposed to normoxia (21% O2), while each treatment group was exposed to hyperoxia (75% O2) for 1, 3, 7, and 14 days. Blood and heart samples were used for blood gas analysis and hematology test, also for catalase specific activity measurement, GSH level, and MDA level measurement. Results: Blood gas analysis of pO2, pCO2, and HCO3 were increased, while the O2 saturation and all hematological parameters were decreased. Plasma and cardiac tissue’s catalase specific activity increased in day 1 to day 7 but declined in day 14. Cardiac tissue’s GSH has the same result. Plasma GSH level increased in day 1 but decreased afterward. MDA level in plasma and cardiac tissue increased significantly since day 1.Conclusion: Hyperoxia causes oxidative stress, marked by the increase of oxidative stress-related markers, and partially compensated respiratory acidosis.
{"title":"CHANGES ON OXIDATIVE STRESS-RELATED BIOMARKERS IN PLASMA AND CARDIAC TISSUE DUE TO PROLONGED EXPOSURE TO NORMOBARIC HYPEROXIA","authors":"Maria Christina Dwiyanti, R. Benettan, F. Wandy, M. Lirendra, F. Ferdinal, D. Limanan","doi":"10.32889/actabioina.v2i1.31","DOIUrl":"https://doi.org/10.32889/actabioina.v2i1.31","url":null,"abstract":"Background: Hyperoxia is a state of oversupply of oxygen in tissues and organs that can increase reactive oxygen species (ROS). When antioxidants cannot balance ROS levels, oxidative stress occurs. Catalase and reduced glutathione (GSH) are two of the antioxidants that can be very useful to counteract ROS. Increased production of ROS subsequently results in lipids damage and generates malondialdehyde (MDA). ROS interaction with cardiac cells causes remodeling thus leads to heart failure.Objectives: The purpose of this study was to find out the changes on oxidative stress-related biomarkers in plasma and cardiac tissue. Methods: Sprague Dawley rats were divided into 5 groups (n=6/group). Control group was exposed to normoxia (21% O2), while each treatment group was exposed to hyperoxia (75% O2) for 1, 3, 7, and 14 days. Blood and heart samples were used for blood gas analysis and hematology test, also for catalase specific activity measurement, GSH level, and MDA level measurement. Results: Blood gas analysis of pO2, pCO2, and HCO3 were increased, while the O2 saturation and all hematological parameters were decreased. Plasma and cardiac tissue’s catalase specific activity increased in day 1 to day 7 but declined in day 14. Cardiac tissue’s GSH has the same result. Plasma GSH level increased in day 1 but decreased afterward. MDA level in plasma and cardiac tissue increased significantly since day 1.Conclusion: Hyperoxia causes oxidative stress, marked by the increase of oxidative stress-related markers, and partially compensated respiratory acidosis.","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128311703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-21DOI: 10.32889/actabioina.v2i1.34
Syazili Mustofa, Fauziah Hanif
Background: The mangrove bark extract (Rhizophora apiculata) is known to have the ability to inhibit the formation of free radicals, act as antioxidants, and anti-inflammatory. Objective: This study was attempted to investigate the potency of Rhizophora apiculata bark extracts as an antioxidant to protect rat testes from the damage due to cigarette smoke exposure. Methods: An experimental study using a posttest-only control group design was employed. Samples consisted of 25 male rats divided into 5 groups, namely K (-) not treated, K (+) exposed to cigarette smoke without the administration of mangrove bark extract, groups P1, P2, and P3 were exposed to cigarette smoke and each group received a dose of Rhizophora apiculata bark extracts every day for 30 days. Furthermore, P1 obtained 28.275 mg/KgBW, P2 was about 56.55 mg/kgBW, and P3 got 113.10 mg/kgBW.Results: Analysis using One Way ANOVA showed that there were significant effects of administration of extracts on the average number of primary spermatocytes and the thickness of the seminiferous tubules in the rats that have been exposed to cigarette smoke when compared to controls. The dose of extract that has the best effect was 113.10 mg/kgBW.Conclusion: Rhizophora apiculata bark extract is indicated to have a protective effect that can prevent damage in rats testes exposed to cigarette smoke.
{"title":"THE PROTECTIVE EFFECT OF RHIZOPHORA APICULATA BARK EXTRACT AGAINST TESTICULAR DAMAGE INDUCED BY CIGARETTE SMOKE IN MALE RATS","authors":"Syazili Mustofa, Fauziah Hanif","doi":"10.32889/actabioina.v2i1.34","DOIUrl":"https://doi.org/10.32889/actabioina.v2i1.34","url":null,"abstract":"Background: The mangrove bark extract (Rhizophora apiculata) is known to have the ability to inhibit the formation of free radicals, act as antioxidants, and anti-inflammatory. Objective: This study was attempted to investigate the potency of Rhizophora apiculata bark extracts as an antioxidant to protect rat testes from the damage due to cigarette smoke exposure. Methods: An experimental study using a posttest-only control group design was employed. Samples consisted of 25 male rats divided into 5 groups, namely K (-) not treated, K (+) exposed to cigarette smoke without the administration of mangrove bark extract, groups P1, P2, and P3 were exposed to cigarette smoke and each group received a dose of Rhizophora apiculata bark extracts every day for 30 days. Furthermore, P1 obtained 28.275 mg/KgBW, P2 was about 56.55 mg/kgBW, and P3 got 113.10 mg/kgBW.Results: Analysis using One Way ANOVA showed that there were significant effects of administration of extracts on the average number of primary spermatocytes and the thickness of the seminiferous tubules in the rats that have been exposed to cigarette smoke when compared to controls. The dose of extract that has the best effect was 113.10 mg/kgBW.Conclusion: Rhizophora apiculata bark extract is indicated to have a protective effect that can prevent damage in rats testes exposed to cigarette smoke.","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114609229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-21DOI: 10.32889/actabioina.v2i1.32
Beauty Novianty, E. Amalia, Z. Maritska, Yuwono Yuwono, Lusia Hayati
Background: Over the past decade, numbers of Carbapenemase Producing-Carbapenem Resistant Enterobacteriaceae (CP-CRE) has been increasing worldwide and it has been becoming a threat because of its resistance against carbapenem which is considered as the “last resort” antibiotic. Therapy options for its infection are still limited. Aminoglycoside serves as one of the most commonly used antibiotics, but the resistance against it has already been presented for a long time. Aminoglycoside Modifying Enzyme (AME) is the most important resistance mechanism against aminoglycoside. AAC(6’)-Ib enzyme is one of the most common AME produced by the gram-negative bacteria.Objectives: This study wished to identify the gene of this enzyme among CRE isolated from infected Indonesian patients in Dr. Mohammad Hoesin Hospital Palembang.Methods: Twenty-eight isolates collected from CRE-infected patients identified by Vitek 2 Compact (bioMerieux, USA) in dr. Mohammad Hoesin Hospital Palembang during September—November 2017. AAC(6’)-Ib gene was identified using PCR method, then visualize by electrophoresis. The result is then analyzed by comparing it with a susceptibility test. Results: Out of 28 samples, AAC(6’)-Ib is identified in 22 (78.57%) samples. Samples with AAC(6’)-Ib showed to be less resistant to various antibiotics, significantly to amikacin (p=0.023).Conclusion: AAC(6’)-Ib gene is found in most of samples implying its frequent occurrence in Indonesian patients.
背景:在过去的十年中，产碳青霉烯酶-耐碳青霉烯肠杆菌科(CP-CRE)在世界范围内的数量不断增加，并因其对碳青霉烯的耐药性而成为一种威胁，碳青霉烯被认为是“最后手段”的抗生素。其感染的治疗选择仍然有限。氨基糖苷是最常用的抗生素之一，但对氨基糖苷的耐药性早已出现。氨基糖苷修饰酶(AME)是氨基糖苷最重要的耐药机制。AAC(6′)-Ib酶是革兰氏阴性菌最常见的AME之一。目的:本研究旨在鉴定巨港穆罕默德·胡辛医生医院感染的印度尼西亚患者分离的CRE酶的基因。方法:收集2017年9 - 11月在Palembang dr. Mohammad Hoesin医院使用Vitek 2 Compact (bioMerieux, USA)检测的28株cre感染患者。用PCR法鉴定AAC(6’)-Ib基因，然后电泳显示。然后通过将其与药敏试验进行比较来分析结果。结果:在28份样本中，22份(78.57%)样本中鉴定出AAC(6′)-Ib。含有AAC(6′)-Ib的样品对各种抗生素的耐药性较低，对阿米卡星的耐药性较低(p=0.023)。结论:AAC(6’)-Ib基因在大多数样本中均存在，提示该基因在印度尼西亚患者中较为常见。
{"title":"THE FIRST INVESTIGATION OF AAC(6’)-Ib ENZYME IN CARBAPENEM-RESISTANT ENTEROBACTERIACEAE ISOLATED FROM INDONESIAN PATIENTS","authors":"Beauty Novianty, E. Amalia, Z. Maritska, Yuwono Yuwono, Lusia Hayati","doi":"10.32889/actabioina.v2i1.32","DOIUrl":"https://doi.org/10.32889/actabioina.v2i1.32","url":null,"abstract":"Background: Over the past decade, numbers of Carbapenemase Producing-Carbapenem Resistant Enterobacteriaceae (CP-CRE) has been increasing worldwide and it has been becoming a threat because of its resistance against carbapenem which is considered as the “last resort” antibiotic. Therapy options for its infection are still limited. Aminoglycoside serves as one of the most commonly used antibiotics, but the resistance against it has already been presented for a long time. Aminoglycoside Modifying Enzyme (AME) is the most important resistance mechanism against aminoglycoside. AAC(6’)-Ib enzyme is one of the most common AME produced by the gram-negative bacteria.Objectives: This study wished to identify the gene of this enzyme among CRE isolated from infected Indonesian patients in Dr. Mohammad Hoesin Hospital Palembang.Methods: Twenty-eight isolates collected from CRE-infected patients identified by Vitek 2 Compact (bioMerieux, USA) in dr. Mohammad Hoesin Hospital Palembang during September—November 2017. AAC(6’)-Ib gene was identified using PCR method, then visualize by electrophoresis. The result is then analyzed by comparing it with a susceptibility test. Results: Out of 28 samples, AAC(6’)-Ib is identified in 22 (78.57%) samples. Samples with AAC(6’)-Ib showed to be less resistant to various antibiotics, significantly to amikacin (p=0.023).Conclusion: AAC(6’)-Ib gene is found in most of samples implying its frequent occurrence in Indonesian patients.","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"66 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114810696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-31DOI: 10.32889/actabioina.v1i2.16
S. Dewi, W. Mulyawan, S. Wanandi, M. Sadikin
Background: High altitude can cause hypobaric hypoxia (HH), resulted from the lower barometric pressure and hence partial pressure of oxygen. Hypoxia can lead to a lot of deleterious molecular and cellular changes, such as generation of free radicals or reactive oxygen species (ROS). Increasing of ROS can cause oxidative stress if the antioxidant enzyme does not increase simultaneously. Oxidative damage in brain has toxic effect on cognitive functions. Objective: In this study, we investigate effect of acute intermittent HH on oxidative stress and antioxidant enzyme activity in rat brain. Method: Wistar rats divided into 5 groups, consisting control group and four experimental groups which treated to HH. Rats were exposed to simulated HH equivalent to 35.000 feet in hypobaric chamber for 1 minute, repeated once a week. Results: Level of malondialdehyde and carbonyl in rat brain under acute HH increased at HH exposure (group I) compare to control group. These levels decreased afterward at intermittent HH exposure (group II-IV). Specific activity of superoxide dismutase (SOD) shows increasing level at intermittent HH exposure, especially group IV was increasing of SOD level significantly. The increasing pattern of specific activity of catalase was inversely from SOD pattern, but it still has higher activity in intermittent HH compare to control group. Conclusion: Brain tissue seems to be able to perform an adequate adaptive response to hypobaric hypoxia after the training, shown by its significantly decreased MDA and carbonyl level and also increased specific activity of SOD and catalase.
{"title":"The effect of intermittent hypobaric hypoxia on oxidative stress status and antioxidant enzymes activity in rat brain","authors":"S. Dewi, W. Mulyawan, S. Wanandi, M. Sadikin","doi":"10.32889/actabioina.v1i2.16","DOIUrl":"https://doi.org/10.32889/actabioina.v1i2.16","url":null,"abstract":"Background: High altitude can cause hypobaric hypoxia (HH), resulted from the lower barometric pressure and hence partial pressure of oxygen. Hypoxia can lead to a lot of deleterious molecular and cellular changes, such as generation of free radicals or reactive oxygen species (ROS). Increasing of ROS can cause oxidative stress if the antioxidant enzyme does not increase simultaneously. Oxidative damage in brain has toxic effect on cognitive functions. \u0000Objective: In this study, we investigate effect of acute intermittent HH on oxidative stress and antioxidant enzyme activity in rat brain. \u0000Method: Wistar rats divided into 5 groups, consisting control group and four experimental groups which treated to HH. Rats were exposed to simulated HH equivalent to 35.000 feet in hypobaric chamber for 1 minute, repeated once a week. \u0000Results: Level of malondialdehyde and carbonyl in rat brain under acute HH increased at HH exposure (group I) compare to control group. These levels decreased afterward at intermittent HH exposure (group II-IV). Specific activity of superoxide dismutase (SOD) shows increasing level at intermittent HH exposure, especially group IV was increasing of SOD level significantly. The increasing pattern of specific activity of catalase was inversely from SOD pattern, but it still has higher activity in intermittent HH compare to control group. \u0000Conclusion: Brain tissue seems to be able to perform an adequate adaptive response to hypobaric hypoxia after the training, shown by its significantly decreased MDA and carbonyl level and also increased specific activity of SOD and catalase.","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116071073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-31DOI: 10.32889/actabioina.v1i2.17
Muhamad Arif Budiman, M. Sadikin, A. Prijanti
Background: Folate is an important substance used for purine and pyrimidine nucleotide synthesis. One measurement of folate that already establishes is using ELISA (Enzyme-linked immunosorbent assay) method. Folate binding protein is a protein that can bind folate, therefore it considered can be used as a tool that can replace antibody dependent ELISA method. Objectives: The aim of this research was to create a method for folate measurement in serum called Enzyme-labeled protein ligand binding assay (ELPLBA) by replacing antibody as used in ELISA method with folate binding protein (FBP) that purified from the whey of milk. Methods: The method is tested using 20 serum samples and compared to ELISA. Folate binding protein was purified from bovine’s milk using ammonium sulfate up to 90% saturated, DEAE-cellulose anion exchange chromatography and affinity chromatography. SDS-PAGE and western blot were used to establish the protein band of FBP that has molecular weight of ~25-35 kDa. ELPLBA was arranged with stationary phase using aminohexyl-agarose, and folic acid linked on it using carbodiimide. Results: The result show there was no significant difference of folate concentration between ELPLBA (14.804 ± 2.795) and ELISA method (13.859 ± 3.638), p = 0.363. Conclusion: ELPLBA method show similarity for determination of folate in serum which was the same as standard folate measurement (ELISA).
{"title":"Human serum folate can be measured using folate binding protein linked to enzyme-labeled protein ligand binding assay (ELPLBA) as well as ELISA","authors":"Muhamad Arif Budiman, M. Sadikin, A. Prijanti","doi":"10.32889/actabioina.v1i2.17","DOIUrl":"https://doi.org/10.32889/actabioina.v1i2.17","url":null,"abstract":"Background: Folate is an important substance used for purine and pyrimidine nucleotide synthesis. One measurement of folate that already establishes is using ELISA (Enzyme-linked immunosorbent assay) method. Folate binding protein is a protein that can bind folate, therefore it considered can be used as a tool that can replace antibody dependent ELISA method. \u0000Objectives: The aim of this research was to create a method for folate measurement in serum called Enzyme-labeled protein ligand binding assay (ELPLBA) by replacing antibody as used in ELISA method with folate binding protein (FBP) that purified from the whey of milk. \u0000Methods: The method is tested using 20 serum samples and compared to ELISA. Folate binding protein was purified from bovine’s milk using ammonium sulfate up to 90% saturated, DEAE-cellulose anion exchange chromatography and affinity chromatography. SDS-PAGE and western blot were used to establish the protein band of FBP that has molecular weight of ~25-35 kDa. ELPLBA was arranged with stationary phase using aminohexyl-agarose, and folic acid linked on it using carbodiimide. \u0000Results: The result show there was no significant difference of folate concentration between ELPLBA (14.804 ± 2.795) and ELISA method (13.859 ± 3.638), p = 0.363. \u0000Conclusion: ELPLBA method show similarity for determination of folate in serum which was the same as standard folate measurement (ELISA).","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127705635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-31DOI: 10.32889/actabioina.v1i2.18
H R Helmi, F. Ferdinal, A. Prijanti, S. A. Jusman, Frans D Suyatna
Background: Chronic systemic hypoxia is severe environmental stress for the heart and might lead to the development of heart failure. Apelin is an endogenous peptide that has been shown to have various beneficial effects on cardiac function. Apelin appears to have a role to play in the ventricular dysfunction and maintaining the performance of the heart. Objectives: In the present study we want to investigate the adaptive response of heart tissue to chronic systemic hypoxia and the correlation with apelin expression and oxidative stress in rat. Methods: An experimental study was performed using 28 Sprague-Dawley male rats, 8 weeks of age. Rats were divided into 7 groups 4 each, namely control group; normoxia (O2 atmosphere) and the treatment group of hypoxia (8% O2) for 6 hours; 1;3;5;7 and 14 days respectively. Body weight and heart weight were measured at each treatment. Ventricular thickness was measured by caliper, Apelin mRNA was measured using real-time qRT-PCR with Livak formula and malondialdehyde (MDA) level was used to assess oxidative stress due to cardiac tissue hypoxia. Results: Macroscopic exams showed hypertrophy at day 7th. The relative expression of Apelin mRNA in hypoxic heart is decreased at the beginning and then increased, starting from day-7 to day-14. The MDA levels were significantly increased from day-7 and were strongly correlated with relative expression Apelin. Conclusion: It is concluded that the increase of Apelin expression is related to oxidative stress in heart tissue of rats during chronic systemic hypoxia.
{"title":"Expression of apelin is related to oxidative damage in heart tissue of rats during chronic systemic hypoxia","authors":"H R Helmi, F. Ferdinal, A. Prijanti, S. A. Jusman, Frans D Suyatna","doi":"10.32889/actabioina.v1i2.18","DOIUrl":"https://doi.org/10.32889/actabioina.v1i2.18","url":null,"abstract":"Background: Chronic systemic hypoxia is severe environmental stress for the heart and might lead to the development of heart failure. Apelin is an endogenous peptide that has been shown to have various beneficial effects on cardiac function. Apelin appears to have a role to play in the ventricular dysfunction and maintaining the performance of the heart. \u0000Objectives: In the present study we want to investigate the adaptive response of heart tissue to chronic systemic hypoxia and the correlation with apelin expression and oxidative stress in rat. \u0000Methods: An experimental study was performed using 28 Sprague-Dawley male rats, 8 weeks of age. Rats were divided into 7 groups 4 each, namely control group; normoxia (O2 atmosphere) and the treatment group of hypoxia (8% O2) for 6 hours; 1;3;5;7 and 14 days respectively. Body weight and heart weight were measured at each treatment. Ventricular thickness was measured by caliper, Apelin mRNA was measured using real-time qRT-PCR with Livak formula and malondialdehyde (MDA) level was used to assess oxidative stress due to cardiac tissue hypoxia. \u0000Results: Macroscopic exams showed hypertrophy at day 7th. The relative expression of Apelin mRNA in hypoxic heart is decreased at the beginning and then increased, starting from day-7 to day-14. The MDA levels were significantly increased from day-7 and were strongly correlated with relative expression Apelin. \u0000Conclusion: It is concluded that the increase of Apelin expression is related to oxidative stress in heart tissue of rats during chronic systemic hypoxia.","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129415822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-31DOI: 10.32889/actabioina.v1i2.12
Meiliza Indriani, Ya’kub Rahadiyanto, Yusuf Effendi, B. Putera, Zen Hafy
Background: The stem cell transplantation successful influenced by the quality of the umbilical cord blood which includes the number of CD34 + and Total Nucleated Cells (TNC). Objectives: The study aims to determine the correlation between umbilical cord length and it’s diameter, with the number of CD34 + and TNC cells as indicators of the quality of cord blood storage feasibility. Several other factors as maternal age, gestation period, and infant birth weight also examined. Methods: Thirty four of umbilical cords from the delivered woman in Dr. Mohammad Hoesin Hospital (RSMH) Palembang were collected from May to June 2018. The length and diameter of the cord were immediately measured after delivery. The evaluated cells were counted with a flow cytometer at Klaster Stem Cell and Tissue Engineering Research Centre (SCTE) IMERI Faculty of Medicine University of Indonesia (FKUI). Results: Spearman correlation test show that there was no correlation between the length and diameter of the umbilical cord, maternal age, gestational period and infant weight, with the number of CD34 + and TNC cells in the cord (p> 0.05). However, the profile analysis indicated that the longer and larger the diameter of the umbilical cord, the higher concentration of the CD34+ and TNC cells. Conclusion: This study suggested that the younger maternal age, older gestational age, and higher infant birth weight, also normal hemoglobin level, tend to increase the number of CD34+ and TNC cells in the cord blood.
{"title":"The relationship between the umbilical cord length and it's diameter with the total CD34+ and total nucleated cell (TNC) as a parameter of cord blood selection","authors":"Meiliza Indriani, Ya’kub Rahadiyanto, Yusuf Effendi, B. Putera, Zen Hafy","doi":"10.32889/actabioina.v1i2.12","DOIUrl":"https://doi.org/10.32889/actabioina.v1i2.12","url":null,"abstract":"Background: The stem cell transplantation successful influenced by the quality of the umbilical cord blood which includes the number of CD34 + and Total Nucleated Cells (TNC). \u0000Objectives: The study aims to determine the correlation between umbilical cord length and it’s diameter, with the number of CD34 + and TNC cells as indicators of the quality of cord blood storage feasibility. Several other factors as maternal age, gestation period, and infant birth weight also examined. \u0000Methods: Thirty four of umbilical cords from the delivered woman in Dr. Mohammad Hoesin Hospital (RSMH) Palembang were collected from May to June 2018. The length and diameter of the cord were immediately measured after delivery. The evaluated cells were counted with a flow cytometer at Klaster Stem Cell and Tissue Engineering Research Centre (SCTE) IMERI Faculty of Medicine University of Indonesia (FKUI). \u0000Results: Spearman correlation test show that there was no correlation between the length and diameter of the umbilical cord, maternal age, gestational period and infant weight, with the number of CD34 + and TNC cells in the cord (p> 0.05). However, the profile analysis indicated that the longer and larger the diameter of the umbilical cord, the higher concentration of the CD34+ and TNC cells. \u0000Conclusion: This study suggested that the younger maternal age, older gestational age, and higher infant birth weight, also normal hemoglobin level, tend to increase the number of CD34+ and TNC cells in the cord blood.","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132287023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-31DOI: 10.32889/actabioina.v1i2.15
A. Rosdah, Evi Lusiana, M. Reagan, Abdurrahman Akib, Fadhila Khairunnisa, Afkara Husna
Background: Centella asiatica (L.) Urb is a native herb from Asian countries such as India, China, and Indonesia. This herb has been widely used as a cure for various diseases. However, studies investigating the aqueous extract of Centella asiatica as a nootropic in healthy individuals are still very limited. Objective: This study aims to investigate the potential of aqueous extract of Centella asiatica in enhancing cognitive function of healthy male Wistar rats. Methods: Rats were randomly allocated to four treatment groups, i.e. without treatment and aqueous Centella asiatica extract at doses of 200, 400 and 800 mg/kg. To determine enhancement of cognitive function, novel object recognition (NOR) test was conducted after the course of treatment. Acetylcholine content was assessed by enzyme-linked immunosorbent assay. Results: There was a significantly high preference index towards the novel object in the NOR test in groups treated with 200 mg/kg and 800 mg/kg of the aqueous extract compared to control. This was further confirmed by a significant increase of brain acetylcholine content in rats treated with 200 mg/kg of the extract. Conclusion: Therefore, this study confirms that the aqueous extract is effective in enhancing cognitive performance of healthy Wistar rats.
{"title":"Enhancing cognitive function of healthy Wistar rats with aqueous extract of Centella asiatica","authors":"A. Rosdah, Evi Lusiana, M. Reagan, Abdurrahman Akib, Fadhila Khairunnisa, Afkara Husna","doi":"10.32889/actabioina.v1i2.15","DOIUrl":"https://doi.org/10.32889/actabioina.v1i2.15","url":null,"abstract":"Background: Centella asiatica (L.) Urb is a native herb from Asian countries such as India, China, and Indonesia. This herb has been widely used as a cure for various diseases. However, studies investigating the aqueous extract of Centella asiatica as a nootropic in healthy individuals are still very limited. \u0000Objective: This study aims to investigate the potential of aqueous extract of Centella asiatica in enhancing cognitive function of healthy male Wistar rats. \u0000Methods: Rats were randomly allocated to four treatment groups, i.e. without treatment and aqueous Centella asiatica extract at doses of 200, 400 and 800 mg/kg. To determine enhancement of cognitive function, novel object recognition (NOR) test was conducted after the course of treatment. Acetylcholine content was assessed by enzyme-linked immunosorbent assay. \u0000Results: There was a significantly high preference index towards the novel object in the NOR test in groups treated with 200 mg/kg and 800 mg/kg of the aqueous extract compared to control. This was further confirmed by a significant increase of brain acetylcholine content in rats treated with 200 mg/kg of the extract. \u0000Conclusion: Therefore, this study confirms that the aqueous extract is effective in enhancing cognitive performance of healthy Wistar rats.","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133826060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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