Background: Hyperuricemia is characterized by elevated levels of uric acid in the blood, produced from its precursor's xanthine and hypoxanthine via xanthine oxidase. Arcangelisia flava, an herbal medicine containing flavonoids, may decrease uric acid levels by inhibiting the xanthine oxidase. Objective: This study was to determine the effectiveness of ethanol extracts of A. flava leaves and stems in inhibiting xanthine oxidase. Methods: The leaves and stems of A. flava were extracted using ethanol 96%. Xanthine oxidase activity was measured using UV Vis spectrophotometry and represented as IC50 value. Allopurinol was used as a positive control. Results: The IC50 value of xanthine oxidase inhibitory of ethanol extract of A. flava leaves and stems as well as allopurinol was 174.62, 30.44, 24.03 ppm. Conclusion: The stems of A. flava have the activity of lowering uric acid levels better than the leaves.
{"title":"Xanthine oxidase inhibitory activity of Arcangelisia flava","authors":"Fatmawati, Subandrate, Safyudin, Medina Athiah, M Fitra Romadhon, Aulia Firdha Tariza","doi":"10.32889/actabioina.71","DOIUrl":"https://doi.org/10.32889/actabioina.71","url":null,"abstract":"Background: Hyperuricemia is characterized by elevated levels of uric acid in the blood, produced from its precursor's xanthine and hypoxanthine via xanthine oxidase. Arcangelisia flava, an herbal medicine containing flavonoids, may decrease uric acid levels by inhibiting the xanthine oxidase. \u0000Objective: This study was to determine the effectiveness of ethanol extracts of A. flava leaves and stems in inhibiting xanthine oxidase. \u0000Methods: The leaves and stems of A. flava were extracted using ethanol 96%. Xanthine oxidase activity was measured using UV Vis spectrophotometry and represented as IC50 value. Allopurinol was used as a positive control. \u0000Results: The IC50 value of xanthine oxidase inhibitory of ethanol extract of A. flava leaves and stems as well as allopurinol was 174.62, 30.44, 24.03 ppm. \u0000Conclusion: The stems of A. flava have the activity of lowering uric acid levels better than the leaves. \u0000 \u0000 ","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"14 3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116805144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mohammad Zaid Kidwai, Roshan Alam, H. Ahsan, M. Khan, Saba Khan
Background: The incidence and prevalence of type 2 diabetes mellitus (T2DM) is increasing due to obesity and sedentary lifestyles. Increased oxidative stress leads to oxidative damage of biomolecules and decreased antioxidant capacity.Aim: The study was designed to determine the malondialdehyde (MDA) and ferric reducing ability of plasma (FRAP) in T2DM patients at IIMS&R Hospital, Lucknow.Method: The oxidative stress was analyzed in T2DM patients as MDA. The total antioxidant capacity was estimated through the FRAP in T2DM patients and control subjects.Results: The level of MDA was observed to be higher (4.84 ± 1.09) in T2DM patients compared to controls (2.20 ± 0.85). The FRAP was lower among T2DM patients (509.46 ± 126.36) compared to controls (895.62 ± 179.92). The difference between FRAP in T2DM patients and controls was statistically significant (p=0.0001).Conclusion: MDA was found to be higher in T2DM, and the FRAP level was lower in T2DM patients compared to controls, signifying increased oxidative stress and decreased antioxidant level in T2DM patients.
{"title":"Analysis of malondialdehyde and ferric reducing ability of plasma in type 2 diabetes mellitus patients in Lucknow city, India","authors":"Mohammad Zaid Kidwai, Roshan Alam, H. Ahsan, M. Khan, Saba Khan","doi":"10.32889/actabioina.84","DOIUrl":"https://doi.org/10.32889/actabioina.84","url":null,"abstract":"Background: The incidence and prevalence of type 2 diabetes mellitus (T2DM) is increasing due to obesity and sedentary lifestyles. Increased oxidative stress leads to oxidative damage of biomolecules and decreased antioxidant capacity.Aim: The study was designed to determine the malondialdehyde (MDA) and ferric reducing ability of plasma (FRAP) in T2DM patients at IIMS&R Hospital, Lucknow.Method: The oxidative stress was analyzed in T2DM patients as MDA. The total antioxidant capacity was estimated through the FRAP in T2DM patients and control subjects.Results: The level of MDA was observed to be higher (4.84 ± 1.09) in T2DM patients compared to controls (2.20 ± 0.85). The FRAP was lower among T2DM patients (509.46 ± 126.36) compared to controls (895.62 ± 179.92). The difference between FRAP in T2DM patients and controls was statistically significant (p=0.0001).Conclusion: MDA was found to be higher in T2DM, and the FRAP level was lower in T2DM patients compared to controls, signifying increased oxidative stress and decreased antioxidant level in T2DM patients.","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114708663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Kurniawaty, Shina Megaputri, Syazili Mustofa, Soraya Rahmanisa, K. A. Audah, Silvia Andriani
Background: Bruguiera gymnorrhiza mangrove and propolis are often used as natural wound treatments. Its constituent is believed to promote wound healing. Objective: This study aims to explore the effect of topical administration of ethanol extract of Bruguiera gymnorrhiza mangrove leaves and propolis on wound healing activity. Methods: Twenty-four Sprague Dawley rats were randomly assigned to one of four treatment groups (n=6): aqua dest, standard wound medicine, ethanol extract of Bruguiera gymnorrhiza mangrove leaves, and propolis extract. Following the incision, treatment was given once a day for 14 days or until the wound healed. The cut area was observed by measuring the cut length using a ruler. Results: Standard wound medication took 9 days to promote healing, while the negative control (aqua dest) took 11.2 days, the ethanol extract of Bruguiera gymnorrhiza mangrove leaves took 7.3 days, and the propolis extract took 7.9 days. Conclusions: Topical administration of Bruguiera gymnorrhiza mangrove leaves ethanol extract and propolis had equal effects on the healing of wounds. Both are more effective than conventional wound ointments at healing cuts.
{"title":"Ethanol extract of Bruguiera gymnorrhiza mangrove leaves and propolis activity on macroscopic healing of cuts in vivo","authors":"E. Kurniawaty, Shina Megaputri, Syazili Mustofa, Soraya Rahmanisa, K. A. Audah, Silvia Andriani","doi":"10.32889/actabioina.94","DOIUrl":"https://doi.org/10.32889/actabioina.94","url":null,"abstract":"Background: Bruguiera gymnorrhiza mangrove and propolis are often used as natural wound treatments. Its constituent is believed to promote wound healing. \u0000Objective: This study aims to explore the effect of topical administration of ethanol extract of Bruguiera gymnorrhiza mangrove leaves and propolis on wound healing activity. \u0000Methods: Twenty-four Sprague Dawley rats were randomly assigned to one of four treatment groups (n=6): aqua dest, standard wound medicine, ethanol extract of Bruguiera gymnorrhiza mangrove leaves, and propolis extract. Following the incision, treatment was given once a day for 14 days or until the wound healed. The cut area was observed by measuring the cut length using a ruler. \u0000Results: Standard wound medication took 9 days to promote healing, while the negative control (aqua dest) took 11.2 days, the ethanol extract of Bruguiera gymnorrhiza mangrove leaves took 7.3 days, and the propolis extract took 7.9 days. \u0000Conclusions: Topical administration of Bruguiera gymnorrhiza mangrove leaves ethanol extract and propolis had equal effects on the healing of wounds. Both are more effective than conventional wound ointments at healing cuts.","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"60 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126533540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
N. Mudjihartini, R. Paramita, A. M. K. Siregar, Estiana Filzadiyanti, Pungguri Ayu Nega Sarsanti, E. Purwaningsih
Background: Free radicals in excessive concentrations damages cells and accelerate the aging process. Antioxidants found in Centella asiatica (CA) and Acalypha indica (AI) have the potential to prevent oxidative cellular damage. Objective: This study aimed to determine the effect of CA and AI on carbonyl and glutathione levels in the brain of older rats. Methods: 18-month age rats were treated using either AI, CA, or vitamin E. In addition, 18-month age and 2-month age untreated rats were used as a negative control. The brain carbonyl and glutathione levels were measured by Agustyanak and the Elmann method, respectively. Results: Treatment with CA significantly decreased brain carbonyl levels (2.87 nmol/mL) than the control rats (4.54 nmol/mL). Furthermore, treating AI did not reduce the brain carbonyl and GSH levels in aged brain rats. Conclusion: Centella asiatica can reduce the protein destruction that occurs with increasing age.
{"title":"Comparing the effect of Centella asiatica L. and Acalypha indica L. treatment to carbonyl and glutathione level in the brains of old rats","authors":"N. Mudjihartini, R. Paramita, A. M. K. Siregar, Estiana Filzadiyanti, Pungguri Ayu Nega Sarsanti, E. Purwaningsih","doi":"10.32889/actabioina.79","DOIUrl":"https://doi.org/10.32889/actabioina.79","url":null,"abstract":"Background: Free radicals in excessive concentrations damages cells and accelerate the aging process. Antioxidants found in Centella asiatica (CA) and Acalypha indica (AI) have the potential to prevent oxidative cellular damage. \u0000Objective: This study aimed to determine the effect of CA and AI on carbonyl and glutathione levels in the brain of older rats. \u0000Methods: 18-month age rats were treated using either AI, CA, or vitamin E. In addition, 18-month age and 2-month age untreated rats were used as a negative control. The brain carbonyl and glutathione levels were measured by Agustyanak and the Elmann method, respectively. \u0000Results: Treatment with CA significantly decreased brain carbonyl levels (2.87 nmol/mL) than the control rats (4.54 nmol/mL). Furthermore, treating AI did not reduce the brain carbonyl and GSH levels in aged brain rats. \u0000Conclusion: Centella asiatica can reduce the protein destruction that occurs with increasing age.","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126369411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Arfianti Arfianti, F. A. Djojosugito, Maisaroh Maisaroh, Hendra Asputra, Dita Kartika Sari, Tubagus Odih Rhomdani Wahid
Background: Mutations within the hepatitis B virus (HBV) reverse transcriptase (RT) gene have been associated with drug resistance against nucleos(t)ide analogs (NAs). Objective: This study aimed to identify mutations in the RT gene among patients with chronic hepatitis B (CHB) before receiving antiviral therapy and its relationship with the HBV genotypes. Methods: A total of 26 HBV DNA was extracted from the blood plasma of CHB patients. HBV RT gene was amplified and sequenced using the Sanger dideoxy sequencing method. The HBV genotype was determined through phylogenetic analysis using the Maximum Likelihood method. Results: The study subjects comprised 14 CHB patients without complications and 12 CHB patients with cirrhosis/hepatoma. CHB patients with cirrhosis/hepatoma were older than those without complications. The HBV genotypes comprised 15 (57.7%) genotype C and 11 (42.3%) genotype B. All treatment-naïve CHB patients did not demonstrate any classical NA resistance mutations within the RT gene. However, several putative and pretreatment resistance mutations, including F221Y, N238H, and V224I, were high frequency in more than 40% of study subjects. In addition, F221Y and N238H/Q mutations were frequently observed in genotype B, while V224 I was only found in patients infected with genotype C (p=0.000). Conclusions: There was no evidence of classical RT gene mutations associated with NA resistance in treatment-naïve patients with CHB. However, several putative and pretreatment mutations were identified as genotype-specific mutations and may contribute to antiviral resistance against NAs.
{"title":"Putative and pretreatment drug resistance mutations in reverse transcriptase gene among untreated chronic hepatitis B patients at Arifin Achmad Regional District Hospital, Riau, Indonesia","authors":"Arfianti Arfianti, F. A. Djojosugito, Maisaroh Maisaroh, Hendra Asputra, Dita Kartika Sari, Tubagus Odih Rhomdani Wahid","doi":"10.32889/actabioina.68","DOIUrl":"https://doi.org/10.32889/actabioina.68","url":null,"abstract":"Background: Mutations within the hepatitis B virus (HBV) reverse transcriptase (RT) gene have been associated with drug resistance against nucleos(t)ide analogs (NAs). \u0000Objective: This study aimed to identify mutations in the RT gene among patients with chronic hepatitis B (CHB) before receiving antiviral therapy and its relationship with the HBV genotypes. \u0000Methods: A total of 26 HBV DNA was extracted from the blood plasma of CHB patients. HBV RT gene was amplified and sequenced using the Sanger dideoxy sequencing method. The HBV genotype was determined through phylogenetic analysis using the Maximum Likelihood method. \u0000Results: The study subjects comprised 14 CHB patients without complications and 12 CHB patients with cirrhosis/hepatoma. CHB patients with cirrhosis/hepatoma were older than those without complications. The HBV genotypes comprised 15 (57.7%) genotype C and 11 (42.3%) genotype B. All treatment-naïve CHB patients did not demonstrate any classical NA resistance mutations within the RT gene. However, several putative and pretreatment resistance mutations, including F221Y, N238H, and V224I, were high frequency in more than 40% of study subjects. In addition, F221Y and N238H/Q mutations were frequently observed in genotype B, while V224 I was only found in patients infected with genotype C (p=0.000). \u0000Conclusions: There was no evidence of classical RT gene mutations associated with NA resistance in treatment-naïve patients with CHB. However, several putative and pretreatment mutations were identified as genotype-specific mutations and may contribute to antiviral resistance against NAs.","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125796049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Metal nanoclusters (NCs) with outstanding structural and optical properties have been intensively validated for applications in nanomedicine and nanotechnology. Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) is overexpressed in many cancer cells. Objective: The gold nanoclusters conjugated with a single domain antibody targeting CEACAM6 of 2A3 (2A3-AuNCs) were synthesized for the inhibition of cancer cells. Methods: 2A3-AuNCs were prepared via a facile hydrothermal approach. The cell viability was measured by resazurin dye reduction assay. The cell death was analyzed by fluorescence imaging. Results: Structural and optical characterizations demonstrated the successful synthesis of 2A3-AuNCs with a roughly spherical shape and a size of 2.35 nm. The 2A3-AuNCs revealed a maximum fluorescence intensity at 350 nm with a fluorescence quantum yield of 4.0%. The cell viability assay indicated that 2A3-AuNCs could inhibit the growths of cancer cells with overexpressed CEACAM6, including breast cancer MDA-MB-231 and MDA-MB-468 cells. The fluorescence imaging results also demonstrated that 2A3-AuNCs could inhibit the growth of cancer cells with MDA-MB-231 and MDA-MB-468 cells. Conclusion: Combination with the results of cell viability assay and fluorescence imaging, the surface ligand of 2A3 antibody on 2A3-AuNCs exhibited promising inhibition of CEACAM6 overexpressed cancer cells. Our work provides a potential application of AuNCs in cancer therapy.
{"title":"Inhibition of cancer cells using target-specific 2A3 antibody-conjugated gold nanoclusters","authors":"Jui-Chi Kuo, Tsung-Rong Kuo, Fajar Rinawati, Erna Susilowati, Sucipto, Dyah Ika Krisnawati","doi":"10.32889/actabioina.69","DOIUrl":"https://doi.org/10.32889/actabioina.69","url":null,"abstract":"Background: Metal nanoclusters (NCs) with outstanding structural and optical properties have been intensively validated for applications in nanomedicine and nanotechnology. Carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) is overexpressed in many cancer cells. \u0000Objective: The gold nanoclusters conjugated with a single domain antibody targeting CEACAM6 of 2A3 (2A3-AuNCs) were synthesized for the inhibition of cancer cells. \u0000Methods: 2A3-AuNCs were prepared via a facile hydrothermal approach. The cell viability was measured by resazurin dye reduction assay. The cell death was analyzed by fluorescence imaging. \u0000Results: Structural and optical characterizations demonstrated the successful synthesis of 2A3-AuNCs with a roughly spherical shape and a size of 2.35 nm. The 2A3-AuNCs revealed a maximum fluorescence intensity at 350 nm with a fluorescence quantum yield of 4.0%. The cell viability assay indicated that 2A3-AuNCs could inhibit the growths of cancer cells with overexpressed CEACAM6, including breast cancer MDA-MB-231 and MDA-MB-468 cells. The fluorescence imaging results also demonstrated that 2A3-AuNCs could inhibit the growth of cancer cells with MDA-MB-231 and MDA-MB-468 cells. \u0000Conclusion: Combination with the results of cell viability assay and fluorescence imaging, the surface ligand of 2A3 antibody on 2A3-AuNCs exhibited promising inhibition of CEACAM6 overexpressed cancer cells. Our work provides a potential application of AuNCs in cancer therapy.","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114456606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: The ongoing COVID-19 pandemic has led to the emergence of several variants of concern. To rapidly identify those variants, screening samples for whole-genome sequencing (WGS) prioritization could be performed. Objective: We optimized the polymerase chain reaction (PCR) screening method to identify the mutation in spike and ORF1a regions. Methods: We adopted primers targeting mutation in spike and ORF1a region from another study. We optimized the PCR screening method using kits readily available in Indonesia. Firstly, we compared N1 and N2 primers as internal positive control. We also compared GoTaq® 1-Step RT-qPCR System and Indonesia TFRIC-19 BioCOV-19 for the multiplex reaction. We used the optimized composition to screen SARS-CoV-2 positive samples from April – June 2021. Samples with spike and/or ORF1a target failure were subjected to whole genome sequencing (WGS). Results: The results demonstrated the N2 BioCOV-19 reaction as the optimized multiplex PCR composition for spike and ORF1a mutations screening. Whole-genome sequencing has shown that a sample with spike and ORF1a targets failure to be Alpha variant, while other samples with single target failure as non-variants of concern. Therefore, a multiplex RT-PCR composition has been optimized to detect mutation in spike and ORF1a regions. Conclusion: We have optimized a multiplex RT-PCR composition to detect mutation in spike and ORF1a regions.
{"title":"Optimization of multiplex PCR composition to screen for SARS-CoV-2 variants of concern","authors":"M. Savira, E. Asni, R. Kemal","doi":"10.32889/actabioina.58","DOIUrl":"https://doi.org/10.32889/actabioina.58","url":null,"abstract":"Background: The ongoing COVID-19 pandemic has led to the emergence of several variants of concern. To rapidly identify those variants, screening samples for whole-genome sequencing (WGS) prioritization could be performed. \u0000Objective: We optimized the polymerase chain reaction (PCR) screening method to identify the mutation in spike and ORF1a regions. \u0000Methods: We adopted primers targeting mutation in spike and ORF1a region from another study. We optimized the PCR screening method using kits readily available in Indonesia. Firstly, we compared N1 and N2 primers as internal positive control. We also compared GoTaq® 1-Step RT-qPCR System and Indonesia TFRIC-19 BioCOV-19 for the multiplex reaction. We used the optimized composition to screen SARS-CoV-2 positive samples from April – June 2021. Samples with spike and/or ORF1a target failure were subjected to whole genome sequencing (WGS). \u0000Results: The results demonstrated the N2 BioCOV-19 reaction as the optimized multiplex PCR composition for spike and ORF1a mutations screening. Whole-genome sequencing has shown that a sample with spike and ORF1a targets failure to be Alpha variant, while other samples with single target failure as non-variants of concern. Therefore, a multiplex RT-PCR composition has been optimized to detect mutation in spike and ORF1a regions. \u0000Conclusion: We have optimized a multiplex RT-PCR composition to detect mutation in spike and ORF1a regions.","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"185 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134049149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Marisa, L. Hayatie, S. Juliati, E. Suhartono, N. Komari
Background: Stenochlaena palustris, also known as kelakai or lemidi, is frequently linked to anti-inflammatory, anti-bacterial, anti-fungal, and antioxidant properties. S. palustris phytosterols are suggested to suppress the progression of breast cancer. Objective: The objective of this study is to evaluate the potential of phytosterols found in S. palustris to act as estrogen receptor (ER) inhibitors. Methods: Phytosterols (alpha-tocopherol, beta-sitosterol, campesterol, stigmasterol, fucosterol) were docked to estrogen receptor (PDB ID: 7KBS). Molecular docking parameters included Gibb's free energy and interactions between ligand and protein. ADMET properties were analyzed using pkCSM and SwissADME. Results: Alpha-tocopherol showed the highest interaction with the estrogen receptor with ΔG value -8. 9254 kcal/mol (the native ligand, raloxifene, had a G value of -12.052 kcal/mol). Leu387 (hydrogen bond); Phe404 (Phi-phi-T shaped), Leu391, Leu346, Trp383, Leu354, Ala350, Leu525, Leu349 (Alkyl) were among the residues by which a-tocopherol interacted with ER. Alpha-tocopherol has no hepatotoxicity and no skin sensitization. Conclusion: By suppressing ERa, phytosterols from S. palustris may have potential anti-breast cancer activity and may be used to prevent estrogen-dependent human cancers like breast cancer.
{"title":"Molecular docking of phytosterols in Stenochlaena palustris as anti-breast cancer","authors":"D. Marisa, L. Hayatie, S. Juliati, E. Suhartono, N. Komari","doi":"10.32889/actabioina.59","DOIUrl":"https://doi.org/10.32889/actabioina.59","url":null,"abstract":"Background: Stenochlaena palustris, also known as kelakai or lemidi, is frequently linked to anti-inflammatory, anti-bacterial, anti-fungal, and antioxidant properties. S. palustris phytosterols are suggested to suppress the progression of breast cancer. \u0000Objective: The objective of this study is to evaluate the potential of phytosterols found in S. palustris to act as estrogen receptor (ER) inhibitors. \u0000Methods: Phytosterols (alpha-tocopherol, beta-sitosterol, campesterol, stigmasterol, fucosterol) were docked to estrogen receptor (PDB ID: 7KBS). Molecular docking parameters included Gibb's free energy and interactions between ligand and protein. ADMET properties were analyzed using pkCSM and SwissADME. \u0000Results: Alpha-tocopherol showed the highest interaction with the estrogen receptor with ΔG value -8. 9254 kcal/mol (the native ligand, raloxifene, had a G value of -12.052 kcal/mol). Leu387 (hydrogen bond); Phe404 (Phi-phi-T shaped), Leu391, Leu346, Trp383, Leu354, Ala350, Leu525, Leu349 (Alkyl) were among the residues by which a-tocopherol interacted with ER. Alpha-tocopherol has no hepatotoxicity and no skin sensitization. \u0000Conclusion: By suppressing ERa, phytosterols from S. palustris may have potential anti-breast cancer activity and may be used to prevent estrogen-dependent human cancers like breast cancer.","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"104 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116011758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cellular senescence is one of the defense mechanisms of cells against oncogenic signals by permanently stopping the proliferation of the cell. Senescence cells show a similar characteristic, one of them is senescence-associated secretory phenotype (SASP). SASPs secrete various components, divided according to the type of molecule secreted and based on their mechanism of action against target cells. The main components of SASP are pro-inflammatory mediators. SASP performs dual and contradictory roles, which concurrently provides beneficial effects such as tumor suppression due to the termination of proliferation, recruitment of immune cells, and tissue repair. On the other hand, SASP produces detrimental effects on cells undergoing the senescence process as well as cells in the surrounding environment by increasing tumorigenesis. This review article explains the various components of the SASP, the role of SASP in the inflammatory process, tumor suppression, and tumorigenesis.
{"title":"The role of senescence-associated secretory phenotype (SASP) in cellular senescene","authors":"Filda Vionita Irene De Lime, N. Hardiany","doi":"10.32889/actabioina.33","DOIUrl":"https://doi.org/10.32889/actabioina.33","url":null,"abstract":"Cellular senescence is one of the defense mechanisms of cells against oncogenic signals by permanently stopping the proliferation of the cell. Senescence cells show a similar characteristic, one of them is senescence-associated secretory phenotype (SASP). SASPs secrete various components, divided according to the type of molecule secreted and based on their mechanism of action against target cells. The main components of SASP are pro-inflammatory mediators. SASP performs dual and contradictory roles, which concurrently provides beneficial effects such as tumor suppression due to the termination of proliferation, recruitment of immune cells, and tissue repair. On the other hand, SASP produces detrimental effects on cells undergoing the senescence process as well as cells in the surrounding environment by increasing tumorigenesis. This review article explains the various components of the SASP, the role of SASP in the inflammatory process, tumor suppression, and tumorigenesis.","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"63 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132759971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ni Made Wiasty Sukanty, F. C. Iswanti, S. Dewi, M. Faruqi, Alyssa Shafa Andiana, A. Prijanti
Background: Preeclampsia is one of the factors causing the high maternal mortality rate. The risk of morbidity and mortality is higher in Early Onset Preeclampsia (EOPE). Failure of spiral artery remodeling can cause oxidative stress that can inhibit placental development and increase trophoblast apoptosis. Objective: This study aims to analyze the oxidative stress and apoptosis of EOPE placentas. Methods: This study is an observational study with a cross-sectional design. A total of 31 EOPE placentas and 31 normal term placentas were used to measure the concentration of malondialdehyde (MDA) and the relative mRNA expression of FOXO3 and CASP3 using the spectrophotometric and RT-qPCR methods. Results: There was no difference in MDA concentration (p = 0.580) and FOXO3 (p = 0.467) and CASP3 (p = 0.243) mRNA expression in the normal and EOPE groups. There was a strong positive correlation between FOXO3 and CASP3 mRNA expression in the normal (p= 0.0001; r = 0.938) and EOPE groups (p = 0.0001; r = 0.855). There was no correlation between MDA concentration to FOXO3 (p = 0.124; r = 0.282) and CASP3 (p = 0.569; r = 0.106) mRNA expression in normal placenta. There was positive correlation between MDA concentration to FOXO3 (p = 0.016; r = 0.429) and CASP3 mRNA expression in EOPE placenta (p = 0.028; r = 0.395). Conclusion: These results indicate that cell integrity is still maintained through the autophagy process and the level of apoptosis in the EOPE placenta is regulated by ROS through FOXO3.
{"title":"Correlation between malondialdehyde level and FOXO3 and CASP3 mRNA expression changed in early-onset preeclampsia placenta","authors":"Ni Made Wiasty Sukanty, F. C. Iswanti, S. Dewi, M. Faruqi, Alyssa Shafa Andiana, A. Prijanti","doi":"10.32889/actabioina.61","DOIUrl":"https://doi.org/10.32889/actabioina.61","url":null,"abstract":"Background: Preeclampsia is one of the factors causing the high maternal mortality rate. The risk of morbidity and mortality is higher in Early Onset Preeclampsia (EOPE). Failure of spiral artery remodeling can cause oxidative stress that can inhibit placental development and increase trophoblast apoptosis. \u0000Objective: This study aims to analyze the oxidative stress and apoptosis of EOPE placentas. \u0000Methods: This study is an observational study with a cross-sectional design. A total of 31 EOPE placentas and 31 normal term placentas were used to measure the concentration of malondialdehyde (MDA) and the relative mRNA expression of FOXO3 and CASP3 using the spectrophotometric and RT-qPCR methods. \u0000Results: There was no difference in MDA concentration (p = 0.580) and FOXO3 (p = 0.467) and CASP3 (p = 0.243) mRNA expression in the normal and EOPE groups. There was a strong positive correlation between FOXO3 and CASP3 mRNA expression in the normal (p= 0.0001; r = 0.938) and EOPE groups (p = 0.0001; r = 0.855). There was no correlation between MDA concentration to FOXO3 (p = 0.124; r = 0.282) and CASP3 (p = 0.569; r = 0.106) mRNA expression in normal placenta. There was positive correlation between MDA concentration to FOXO3 (p = 0.016; r = 0.429) and CASP3 mRNA expression in EOPE placenta (p = 0.028; r = 0.395). \u0000Conclusion: These results indicate that cell integrity is still maintained through the autophagy process and the level of apoptosis in the EOPE placenta is regulated by ROS through FOXO3.","PeriodicalId":145722,"journal":{"name":"Acta Biochimica Indonesiana","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127143300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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