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Evolution and Phylodynamics of the Hemagglutinin Protein of Influenza A/(H1N1)pdm09 Virus Isolates from India from 2009 to 2020. 2009 - 2020年印度甲型H1N1流感pdm09病毒分离株血凝素蛋白的进化和系统动力学
IF 2.2 4区 医学 Q4 INFECTIOUS DISEASES Pub Date : 2023-07-24 DOI: 10.7883/yoken.JJID.2022.453
Kiruba Ramesh, Kannapiran Ethiraj, Sivasubramanian Srinivasan, Kaveri Krishnasamy, Kumar Rajendran, Gracyfathima Selvaraj, Padmapriya Padmanaban

Influenza A/(H1N1)pdm09 virus evolves through continuous antigenic variation in both surface antigens, such as hemagglutinin (HA) and neuraminidase (NA) proteins, which affect its pathogenicity, the effectiveness of the host immune response, and drug resistance. This study reports the evolution and dynamics of 527 HA protein sequences of influenza A/(H1N1)pdm09 Indian isolates submitted from 2009 to 2020. These isolates were aligned with a reference sequence and 22 sequences representing different clades using MEGA X, and subjected to phylogenetic analysis. The strains were predominantly grouped in clades 6B.1 and 6B.2. Prediction of glycosylation sites using the BioEdit and NetNglyc servers showed 12 glycosylation sites distributed in both the stem and globular head regions of HA. Functional evaluation showed that there were 22 deleterious mutations that could affect the function of HA. In addition, 403 unique mutations were distributed across various isolates, indicating the dynamics of antigenic variation in Indian isolates. These results provide an understanding of the frequency, phylodynamics, and impact of mutations in Indian isolates of influenza A/(H1N1)pdm09 relative to global isolates. Monitoring the genomic evolution of the virus will support studies on strain selection for vaccine development and devising control and prevention measures to manage this respiratory infection.

甲型H1N1流感pdm09病毒通过两种表面抗原(如血凝素(HA)和神经氨酸酶(NA)蛋白)的持续抗原变异而进化,从而影响其致病性、宿主免疫反应的有效性和耐药性。本研究报告了2009 - 2020年提交的甲型H1N1流感pdm09印度分离株527个HA蛋白序列的进化和动态。利用MEGA X将这些分离株与参考序列和22个代表不同支系的序列进行比对,并进行系统发育分析。菌株主要集中在6B支系。2.选b。使用BioEdit和NetNglyc服务器预测糖基化位点显示,12个糖基化位点分布在HA的茎和球状头区域。功能评价结果显示,有22个有害突变可影响HA功能。此外,403个独特的突变分布在不同的分离株中,表明印度分离株的抗原变异动态。这些结果提供了对印度甲型H1N1流感pdm09分离株相对于全球分离株的频率、系统动力学和突变影响的理解。监测病毒的基因组进化将支持研究疫苗开发的毒株选择和制定控制和预防措施,以管理这种呼吸道感染。
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引用次数: 0
A Nosocomial Outbreak Caused by Human Rhinovirus Species A Type 61 in a Welfare Facility in Gunma Prefecture, Japan. 日本群马县一家福利院发生由甲型61型人鼻病毒引起的院内暴发。
IF 2.2 4区 医学 Q4 INFECTIOUS DISEASES Pub Date : 2023-07-24 DOI: 10.7883/yoken.JJID.2023.039
Ryo Shimada, Hiroyuki Tsukagoshi, Rina Kubota, Daisuke Shinoda, Yuri Shinohara, Akio Saito, Fumitaka Inoue, Tadaaki Endo, Nobuhiro Saruki

Human rhinovirus (HRV) infections are generally referred to as the common cold, and are the main cause of mild symptoms. HRV is less frequently implicated in the development of severe respiratory infections. This study reports a nosocomial outbreak of bronchitis and pneumonia caused by HRV in a hospital during the COVID-19 epidemic in September 2022 in Gunma Prefecture, Japan. The patient continued to be symptomatic for nine days. During this outbreak, all 15 residents displayed respiratory symptoms. HRV-A was detected in 12 of the 12 samples, and phylogenetic analysis classified the strain as HRV-A type 61. HRV, COVID-19, and other respiratory infections cannot be differentiated based solely on clinical symptoms. A surveillance system to monitor them is thus needed.

人类鼻病毒(HRV)感染通常被称为普通感冒,是轻微症状的主要原因。HRV较少涉及严重呼吸道感染的发展。本研究报告了2022年9月日本群马县2019冠状病毒病流行期间一家医院HRV引起的支气管炎和肺炎的院内暴发。患者症状持续了9天。在这次爆发期间,所有15名居民都出现了呼吸道症状。12份样本中有12份检测到HRV-A,系统发育分析将该菌株归类为HRV-A 61型。不能仅仅根据临床症状来区分HRV、COVID-19和其他呼吸道感染。因此需要一个监测它们的监测系统。
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引用次数: 0
Distribution of Human Sapovirus Strain Genotypes over the Last Four Decades in Japan: a Global Perspective. 人类萨波病毒株基因型在日本过去四十年的分布:全球视角。
IF 2.2 4区 医学 Q4 INFECTIOUS DISEASES Pub Date : 2023-07-24 DOI: 10.7883/yoken.JJID.2022.704
Yen Hai Doan, Yasutaka Yamashita, Hiroto Shinomiya, Takumi Motoya, Naomi Sakon, Rieko Suzuki, Hideaki Shimizu, Naoki Shigemoto, Seiya Harada, Shunsuke Yahiro, Kyoko Tomioka, Akie Sakagami, Yo Ueki, Rika Komagome, Kyohei Saka, Reiko Okamoto-Nakagawa, Komei Shirabe, Fuminori Mizukoshi, Yono Arita, Kei Haga, Kazuhiko Katayama, Hirokazu Kimura, Masamichi Muramatsu, Tomoichiro Oka

Sapovirus (SaV) infections are a public health problem because they cause acute gastroenteritis in humans of all ages, both sporadically and as outbreaks. However, only a limited amount of SaV sequence information, especially whole-genome sequences for all the SaV genotypes, is publicly available. Therefore, in this study, we determined the full/near-full-length genomic sequences of 138 SaVs from the 2001 to 2015 seasons in 13 prefectures across Japan. The genogroup GI was predominant (67%, n = 92), followed by genogroups GII (18%, n = 25), GIV (9%, n = 12), and GV (6%, n = 9). Within the GI genogroup, four different genotypes were identified: GI.1 (n = 44), GI.2 (n = 40), GI.3 (n = 7), and GI.5 (n = 1). We then compared these Japanese SaV sequences with 3,119 publicly available human SaV sequences collected from 49 countries over the last 46 years. The results indicated that GI.1, and GI.2 have been the predominant genotypes in Japan, as well as in other countries, over at least four decades. The 138 newly determined Japanese SaV sequences together with the currently available SaV sequences, could facilitate a better understanding of the evolutionary patterns of SaV genotypes.

萨波病毒(SaV)感染是一个公共卫生问题,因为它们在所有年龄段的人中引起急性胃肠炎,既有零星的,也有暴发的。然而,只有有限数量的SaV序列信息,特别是所有SaV基因型的全基因组序列是公开的。因此,在本研究中,我们确定了日本13个县2001年至2015年季节138个sav的全/近全长基因组序列。GI基因组占主导地位(67%,n = 92),其次是GII基因组(18%,n = 25)、GIV基因组(9%,n = 12)和GV基因组(6%,n = 9)。在GI基因组中,鉴定出四种不同的基因型:GI.1 (n = 44)、GI.2 (n = 40)、GI.3 (n = 7)和GI.5 (n = 1)。然后,我们将这些日本SaV序列与过去46年来从49个国家收集的3119个公开的人类SaV序列进行了比较。结果表明,GI.1和GI.2在至少40年的时间里一直是日本以及其他国家的主要基因型。新确定的138个日本SaV序列和现有的SaV序列,有助于更好地了解SaV基因型的进化模式。
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引用次数: 2
Prevalence of Sapovirus and Astrovirus in Pediatric Infectious Gastroenteritis Surveillance in Kobe City, Japan, 2016-2019. 神户市2016-2019年儿童感染性胃肠炎病毒和星状病毒流行监测
IF 2.2 4区 医学 Q4 INFECTIOUS DISEASES Pub Date : 2023-07-24 DOI: 10.7883/yoken.JJID.2023.037
Takeshi Hanafusa, Kentaro Arikawa, Yoshihiko Tanimoto

Sapovirus (SaV) and astrovirus (AstV) are important viral causes of acute gastroenteritis. From 2016 to 2019, 172 stool samples were collected from children with gastroenteritis in Kobe, Japan for sentinel surveillance of infectious gastroenteritis. In this study, we tested 53 of the 172 stool samples that tested negative for other enteric viruses to determine the prevalence of SaV and AstV. The samples were screened for SaV and AstV using real-time polymerase chain reaction. Positive samples were genotyped by sequencing and genetic analysis of partial regions of the capsid and RNA-dependent RNA polymerase. Of the 53 samples tested, 19 (35.8%) were positive for SaV, and three (5.7%) were positive for AstV. Of the total samples, 11.0% (19/172) and 1.7% (3/172) were positive for SaV and AstV, respectively. The most frequently detected genotype of SaV was GI.1, followed by GII.3. The AstV genotypes were MAstV1.1 and MAstV1.4. This study indicates that SaV and AstV are important causes of viral gastroenteritis in children.

腺病毒(SaV)和星状病毒(AstV)是引起急性胃肠炎的重要病毒。2016 - 2019年,在日本神户收集172例肠胃炎患儿粪便样本,对感染性肠胃炎进行哨点监测。在这项研究中,我们对172个粪便样本中的53个进行了其他肠道病毒阴性检测,以确定SaV和asv的流行程度。采用实时聚合酶链反应对样品进行SaV和AstV的筛选。阳性样品通过衣壳和RNA依赖性RNA聚合酶部分区域的测序和遗传分析进行基因分型。53份检测样本中,SaV阳性19份(35.8%),AstV阳性3份(5.7%)。SaV和AstV阳性率分别为11.0%(19/172)和1.7%(3/172)。最常见的SaV基因型为GI.1,其次为gi .3。AstV基因型分别为MAstV1.1和MAstV1.4。本研究提示SaV和AstV是儿童病毒性胃肠炎的重要病因。
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引用次数: 0
The Ubiquitination of RIPK2 is Mediated by Peli3 and Negatively Regulates the Onset of Infectious Osteomyelitis. RIPK2的泛素化是由Peli3介导的,并负向调控感染性骨髓炎的发生。
IF 2.2 4区 医学 Q4 INFECTIOUS DISEASES Pub Date : 2023-07-24 DOI: 10.7883/yoken.JJID.2022.622
Lixiang Le, Haojie Shan, Yiwei Lin, Wenyang Xia, Xin Ma, Chaolai Jiang, Zhongmin Shi, Youjia Xu

Osteomyelitis is the infection and destruction of the bone. To date, there is no universal protocol for its treatment. Receptor-interacting serine/threonine-protein kinase 2 (RIPK2) has been implicated in osteomyelitis development. However, the detailed mechanism remains unknown. Here, 6-8w wild-type or Pellino E3 Ubiquitin Protein Ligase Family Member 3 (Peli3)-deficient mice were injected with Staphylococcus aureus to induce osteomyelitis. RAW264.7 cells or bone marrow-derived macrophages isolated from mice were treated with lipopolysaccharide (LPS). Knocking down Peli3 in RAW264.7 cells increased the expression of inflammatory cytokines (interleukin-1β, interleukin-6, and tumor necrosis factor-α) after LPS stimulation. Inflammation was also activated in S. aureus-induced Peli3-deficient mice. Moreover, S. aureus-infected Peli3-deficient mice also displayed more severe symptoms of osteomyelitis than S. aureus-infected wild-type mice. Moreover, Peli3 targets and degrades RIPK2 through K48-linked ubiquitination, and negatively modulates osteomyelitis by degrading RIPK2. Our data further expands the current understanding of RIPK2 in osteomyelitis, and suggests that RIPK2 might serve as a novel therapeutic target for treating osteomyelitis.

骨髓炎是对骨骼的感染和破坏。到目前为止,还没有通用的治疗方案。受体相互作用丝氨酸/苏氨酸蛋白激酶2 (RIPK2)与骨髓炎的发展有关。然而,其具体机制尚不清楚。本研究采用6-8w野生型或Pellino E3泛素蛋白连接酶家族成员3 (Peli3)缺陷小鼠注射金黄色葡萄球菌诱导骨髓炎。用脂多糖(LPS)处理小鼠RAW264.7细胞或骨髓源性巨噬细胞。在LPS刺激后,RAW264.7细胞中敲低Peli3可增加炎症因子(白细胞介素-1β、白细胞介素-6和肿瘤坏死因子-α)的表达。金黄色葡萄球菌诱导的peli3缺陷小鼠的炎症也被激活。此外,金黄色葡萄球菌感染的peli3缺陷小鼠比金黄色葡萄球菌感染的野生型小鼠表现出更严重的骨髓炎症状。此外,Peli3通过k48相关的泛素化作用靶向并降解RIPK2,并通过降解RIPK2负向调节骨髓炎。我们的数据进一步扩展了目前对RIPK2在骨髓炎中的理解,并表明RIPK2可能作为治疗骨髓炎的新靶点。
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引用次数: 0
Role of Viral Load and Host Cytokines in Determining the Disease Severity of Respiratory Syncytial Virus-Associated Acute Lower Respiratory Tract Infections in Children. 病毒载量和宿主细胞因子在确定儿童呼吸道合胞病毒相关急性下呼吸道感染疾病严重程度中的作用
IF 2.2 4区 医学 Q4 INFECTIOUS DISEASES Pub Date : 2023-07-24 DOI: 10.7883/yoken.JJID.2022.673
Subhabrata Sarkar, Radha Kanta Ratho, Meenu Singh, Mini Pritam Singh, Amarjeet Singh, Megha Sharma

Respiratory syncytial virus (RSV) is a major cause of acute lower respiratory tract infections (ALRTIs). In this study, we aimed to evaluate the role of viral load, cytokines, matrix metalloproteinase 9 (MMP-9), and tissue inhibitor of metalloproteinase 1 (TIMP-1) in determining the severity of RSV disease and identify potential biomarkers of disease severity. A total of 142 patients with RSV infection (aged between 2 months and 5 years) who presented with ALRTI between December 2013 and March 2016 were enrolled. Their nasopharyngeal aspirates were subjected to RSV viral load quantification, and local cytokine levels of interleukin 6 (IL-6), tumor necrosis factor α (TNF-α), IL-17A, interferon γ (IFN-γ), and IL-10 were determined using a cytokine bead array. The levels of MMP-9 and TIMP-1 in 109 aspirates were calculated using Quantikine ELISA. These parameters were compared for different disease severity categories. A higher viral load and increased levels of TNF-α, MMP-9, and MMP-9:TIMP-1 were associated with greater severity of disease; whereas levels of IL-17A, IFN-γ, and IFN-γ:IL-10 were associated with disease resolution. When defining the transition from non-severe to severe disease, MMP-9 had a sensitivity and specificity of 89.7% and 85.4%, respectively. Moreover, MMP-9:TIMP-1 had a sensitivity and specificity of 87.2% and 76.8%, respectively. Hence, MMP-9, MMP-9:TIMP-1, TNF-α, and IL-10 could serve as potential biomarkers for disease progression in RSV-infected children.

呼吸道合胞病毒(RSV)是急性下呼吸道感染(ALRTIs)的主要原因。在这项研究中,我们旨在评估病毒载量、细胞因子、基质金属蛋白酶9 (MMP-9)和金属蛋白酶1组织抑制剂(TIMP-1)在确定RSV疾病严重程度中的作用,并确定疾病严重程度的潜在生物标志物。在2013年12月至2016年3月期间出现呼吸道合胞病毒感染的142例患者(年龄在2个月至5岁之间)被纳入研究。对患者的鼻咽分泌物进行RSV病毒载量定量检测,并用细胞因子头阵列检测局部细胞因子IL-6 (IL-6)、肿瘤坏死因子α (TNF-α)、IL-17A、干扰素γ (IFN-γ)和IL-10水平。采用定量酶联免疫吸附试验(Quantikine ELISA)计算109例抽吸者的MMP-9和TIMP-1水平。对不同疾病严重程度类别的这些参数进行比较。较高的病毒载量和升高的TNF-α、MMP-9和MMP-9:TIMP-1水平与更严重的疾病相关;而IL-17A、IFN-γ和IFN-γ:IL-10的水平与疾病消退有关。在定义从非严重疾病到严重疾病的转变时,MMP-9的敏感性和特异性分别为89.7%和85.4%。MMP-9:TIMP-1的敏感性和特异性分别为87.2%和76.8%。因此,MMP-9、MMP-9:TIMP-1、TNF-α和IL-10可以作为rsv感染儿童疾病进展的潜在生物标志物。
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引用次数: 0
Trends of Mismatches in Real-Time RT-PCR Assays Developed by the National Institute of Infectious Diseases, Japan for Omicron Variant of Severe Acute Respiratory Syndrome Coronavirus 2. 日本国立传染病研究所开发的严重急性呼吸综合征冠状病毒组粒变异实时RT-PCR检测方法的错配趋势
IF 2.2 4区 医学 Q4 INFECTIOUS DISEASES Pub Date : 2023-05-24 DOI: 10.7883/yoken.JJID.2022.556
Kazuya Shirato, Makoto Ujike, Miyuki Kawase

The Omicron variant of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in late 2021 and gradually overtook the Delta variant, which was the predominant variant at that time. The Omicron variant has been consecutively replaced by related sublineages. The real-time RT-PCR assays developed by the National Institute of Infectious Diseases (NIID), Japan (i.e., the NIID-N2 and NIID-S2 assays) are the reference assays that have been used in Japan since the outbreak of SARS-CoV-2. To evaluate the applicability of the NIID assays for the Omicron variants, trends in the prevalence of nucleotide mismatches in the primer/probe sequences were traced using sequences registered in the Global Initiative on Sharing Avian Influenza Data database. Approximately 99% of the deposited Omicron variant sequences did not have any mismatches in the NIID assay primer/probes from January to August 2022. This indicates that the NIID assays have been able to detect the changing SARS-CoV-2 Omicron variants.

严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)的Omicron变体于2021年底出现,并逐渐取代当时占主导地位的Delta变体。基因组变体已连续被相关的亚谱系所取代。日本国立传染病研究所(NIID)开发的实时RT-PCR检测方法(即NIID- n2和NIID- s2检测方法)是自SARS-CoV-2爆发以来日本使用的参考检测方法。为了评估NIID检测对Omicron变异的适用性,利用全球共享禽流感数据倡议数据库中注册的序列,追踪了引物/探针序列中核苷酸错配的流行趋势。在2022年1月至8月期间,大约99%的沉淀的Omicron变异序列在NIID检测引物/探针中没有任何错配。这表明NIID检测已经能够检测到不断变化的SARS-CoV-2基因组变体。
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引用次数: 0
Surveillance of the Antimicrobial Susceptibility and Molecular Characteristics of Neisseria gonorrhoeae Isolates Collected in Changsha, China from 2016 to 2021. 2016 - 2021年长沙市淋病奈瑟菌分离株药敏及分子特征监测
IF 2.2 4区 医学 Q4 INFECTIOUS DISEASES Pub Date : 2023-05-24 DOI: 10.7883/yoken.JJID.2022.532
Qianqin Yuan, Shiya Shi, Yufeng Dai, Mengjie Jiang, Ping Jiang, Danning Xu, Qinglin Liu, Chuanhao Jiang, Xinwu Guo, Hongzhi Chen, Lingli Tang

Antibiotic treatment is critical for individuals infected with gonorrhea and preventing disease transmission. This study aimed to analyze the antimicrobial susceptibility and molecular epidemiological characteristics of Neisseria gonorrhoeae isolates in Changsha, China. A total of 271 N.gonorrhoeae isolates collected from the clinical laboratories of two hospitals between 2016 and 2021 were analyzed for antimicrobial susceptibility using the agar dilution method. N. gonorrhoeae multi-antigen sequence typing (NG-MAST) was conducted for genotyping, and phylogenetic analysis was performed using the porB and tbpB sequences. The results showed that antimicrobial resistance against ciprofloxacin, tetracycline, and penicillin was high, and these drugs are no longer recommended for the treatment of gonorrhea. All isolates were susceptible to spectinomycin. However, in 2016-2021, a total of 15 (5.5%) ceftriaxone (CRO)-resistant strains and 31 (11.4%) isolates with decreased susceptibility to CRO were found, and the resistance rate to azithromycin had reached 7.1% in 2016-2017. Epidemiologically, the mosaic penA allele was identified in all CRO-resistant isolates. Based on NG-MAST, ST5061 was the most prevalent ST. Phylogenetic analysis suggested that the resistant isolates did not cluster independently. Despite focus on the local situation, this study raises the need for better gonorrhea medication and highlights that CRO may not be adequate as first-line treatment for gonorrhea in Changsha.

抗生素治疗对感染淋病的个体和预防疾病传播至关重要。本研究旨在分析长沙地区淋病奈瑟菌分离株的抗菌药物敏感性和分子流行病学特征。采用琼脂稀释法对2016 - 2021年两所医院临床实验室采集的271株淋病奈瑟菌进行药敏分析。采用淋病奈瑟菌多抗原序列分型(NG-MAST)进行基因分型,并用porB和tbpB序列进行系统发育分析。结果显示,对环丙沙星、四环素和青霉素的耐药性较高,这些药物已不再推荐用于治疗淋病。所有分离株均对大观霉素敏感。然而,2016-2021年共发现头孢曲松(CRO)耐药菌株15株(5.5%),CRO敏感性降低的菌株31株(11.4%),2016-2017年对阿奇霉素的耐药率为7.1%。流行病学上,在所有抗cro菌株中均鉴定出花叶型penA等位基因。NG-MAST检测结果显示ST5061是最常见的ST.菌株,系统发育分析表明耐药菌株不是独立聚集的。尽管本研究着眼于当地情况,但提出了改善淋病药物治疗的必要性,并强调CRO可能不足以作为长沙市淋病的一线治疗方法。
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引用次数: 1
Possible Transmission of Severe Fever with the Thrombocytopenia Syndrome Virus to an Individual Who Buried an Infected Cat. 重症发热伴血小板减少综合征病毒可能传播给掩埋受感染猫的人。
IF 2.2 4区 医学 Q4 INFECTIOUS DISEASES Pub Date : 2023-05-24 DOI: 10.7883/yoken.JJID.2022.425
Hirohisa Mekata, Takeshi Kawaguchi, Kosho Iwao, Kazumi Umeki, Kentaro Yamada, Kunihiko Umekita, Tamaki Okabayashi

Severe fever with thrombocytopenia syndrome (SFTS) is caused by the severe fever with thrombocytopenia syndrome virus (SFTSV). Although SFTS is a fatal tick-borne zoonosis, it can infect humans without tick bite exposure. Recently, direct transmission of SFTSV from companion pets to humans has become a major problem. We present a case of SFTSV transmission from a dead community cat to a woman who buried the cat in Miyazaki Prefecture, Japan. The community cat died without a diagnosis of SFTS, and the woman buried it without taking any precautions. She developed symptoms of SFTS 9 days later. The woman tested positive for SFTS viral RNA and anti-SFTSV antibodies. The cat's carcass was exhumed, and tissue samples were collected to confirm the viral infection. Numerous copies of viral RNA were detected. The SFTSV M segment sequences in the cat and the woman were 100% homologous. The woman claimed that she had touched blood that had leaked from the cat's body while burying it. However, she could have been infected while transporting the cat to the animal hospital. This study highlights the risk of SFTSV infection from contact with sick or dead community cats.

发热伴血小板减少综合征(SFTS)是由发热伴血小板减少综合征病毒(SFTSV)引起的。虽然SFTS是一种致命的蜱传人畜共患病,但它可以在没有蜱叮咬的情况下感染人类。最近,从伴侣宠物到人类的SFTSV直接传播已成为一个主要问题。我们报告了日本宫崎县一只死猫向一名埋葬猫的妇女传播SFTSV的病例。这只社区猫在没有被诊断出患有SFTS的情况下死亡,该妇女在没有采取任何预防措施的情况下埋葬了它。9天后出现SFTS症状。该妇女的SFTS病毒RNA和抗sftsv抗体检测呈阳性。猫的尸体被挖掘出来,并收集了组织样本以确认病毒感染。检测到大量的病毒RNA拷贝。猫和妇女的SFTSV M片段序列同源性为100%。这名女子声称,她在埋葬猫的时候接触到了从猫身上流出的血。然而,她可能是在运送猫到动物医院的过程中被感染的。这项研究强调了SFTSV感染的风险来自与生病或死亡的社区猫的接触。
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引用次数: 2
RBF Protein with MA103 Adjuvant Elicited Protective Immunity against Human Respiratory Syncytial Virus in BALB/c Mice. 含MA103佐剂的RBF蛋白诱导BALB/c小鼠对人呼吸道合胞病毒的保护性免疫
IF 2.2 4区 医学 Q4 INFECTIOUS DISEASES Pub Date : 2023-05-24 DOI: 10.7883/yoken.JJID.2022.476
Qiongqiong Fang, Hai Li, Hu Ren, Lei Cao, Hongqiao Hu, Yan Zhang, Wenbo Xu

The development of a vaccine against human respiratory syncytial virus (HRSV) has been hampered by enhanced respiratory disease due to the Th2-biased immune response. In the present study, MA103 and aluminum phosphate (Adju-Phos) adjuvants were used to verify the immunogenicity of the recombinant fusion (RBF) protein (F protein expressed by Escherichia coli). Both adjuvants significantly increased the neutralizing antibody titer and number of interferon gamma (IFN-γ)-secreting CD4+ T cells in mice. Based on the immunoglobulin G1 (IgG1)/IgG2a and IFN-γ/interleukin 4-secreting CD4+ T cell ratio, however, MA103 significantly enhanced the Th1-biased immune response. The pathological damage to the lung in the RBF/MA103 group was less than what was seen in the RBF/Adju-Phos group. Additionally, the number of HRSV copies in the lungs of the RBF/MA103 group decreased by approximately 3 × log10. These results suggested that MA103 provides better protection against HRSV in mice.

人类呼吸道合胞病毒(HRSV)疫苗的开发一直受到th2偏向性免疫反应导致的呼吸道疾病加剧的阻碍。本研究采用MA103和磷酸铝(aji - phos)佐剂对重组融合蛋白(大肠杆菌表达的F蛋白)的免疫原性进行了验证。两种佐剂均显著增加小鼠体内分泌干扰素γ (IFN-γ)的CD4+ T细胞的中和抗体滴度和数量。然而,基于免疫球蛋白G1 (IgG1)/IgG2a和IFN-γ/白细胞介素4分泌CD4+ T细胞的比例,MA103显著增强了th1偏向性免疫应答。RBF/MA103组肺病理损伤明显小于RBF/ jul - phos组。此外,RBF/MA103组肺中HRSV拷贝数减少了约3 × log10。这些结果表明MA103对小鼠HRSV具有更好的保护作用。
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引用次数: 0
期刊
Japanese journal of infectious diseases
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