Pub Date : 2024-01-01DOI: 10.33899/ijvs.2023.142183.3161
D. A. Darwish, H. Masoud, M. S. Helmy, W. T. Abbas, R. Shaapan, N. Toaleb, M. A. Ibrahim
Moniezia expansa worms are a significant source of alkaline phosphatase (ALP) and acetylcholinesterase (AChE) enzymes. The current study presents a simple and reproducible ALP and AChE purification method from Moniezia expansa helminthes by precipitating the proteins with ammonium sulfate and chromatography on the Sephacryl S-300 column. The M. expansa ALP purified at 1070.8 U/mg, displaying 6.0 purification folds and 53.6% yield, while M. expansa AChE is at 5250 U/mg, displaying 2.0 purification folds and 43% yield. The M. expansa ALP isoenzyme displayed its optimum activity at pH 9.6, while the M. expansa AChE isoenzyme displayed its optimum activity at pH 8.0. The affinity of M. expansa ALP for several substrates revealed that p-nitrophenyl phosphate preferentially cleaved with a Km value of 4.4 mM. M. expansa AChE preferentially cleaved acetylthiocholine iodide with a Km value of 0.9 mM. M. expansa ALP is strongly stimulated with Co 2+ , Mn 2+ , Ni 2+ , and Mg 2+ and reduced with Zn 2+ , Cu 2+ , Ca 2+ , EDTA and DTT. On the other hand, M. expansa AChE is significantly induced with Co 2+ , Zn 2+ , and Ni 2+ and inhibited with Mg 2+ , Ca 2+ , EDTA, 1,10-phenanthroline and eserine. The antisera of the purified M. expansa ALP and AChE found effective for determining the two enzymes in different unknown sera from different animal species, including humans, sheep and fish. These results may provide a possible future application of such enzymes in producing ALP and AChE-coated ELISA plates for research purposes.
扩张莫尼茨绦虫是碱性磷酸酶（ALP）和乙酰胆碱酯酶（AChE）的重要来源。本研究提出了一种简单、可重复的扩张莫尼茨绦虫 ALP 和 AChE 纯化方法，即用硫酸铵沉淀蛋白质，然后用 Sephacryl S-300 色谱柱进行层析。扩张莫尼茨绦虫ALP的纯化率为1070.8 U/mg，纯化倍数为6.0，产率为53.6%；扩张莫尼茨绦虫AChE的纯化率为5250 U/mg，纯化倍数为2.0，产率为43%。扩张麦氏ALP同工酶在pH值为9.6时显示出最佳活性，而扩张麦氏AChE同工酶在pH值为8.0时显示出最佳活性。扩张莫氏ALP对几种底物的亲和力显示，对硝基苯磷酸优先裂解，Km值为4.4 mM。扩张莫氏 AChE 优先裂解碘化乙酰硫代胆碱，Km 值为 0.9 mM。扩张莫氏菌的 ALP 受 Co 2+ 、Mn 2+ 、Ni 2+ 和 Mg 2+ 的强烈刺激，而受 Zn 2+ 、Cu 2+ 、Ca 2+ 、EDTA 和 DTT 的强烈刺激则会降低。另一方面，Co 2+ 、Zn 2+ 和 Ni 2+ 能显著诱导扩张麦芽糖乙酰胆碱酯酶，而 Mg 2+ 、Ca 2+ 、EDTA、1,10-菲罗啉和丝氨酸能抑制该酶。纯化的扩张莫氏杆菌 ALP 和 AChE 抗血清可有效测定不同动物物种（包括人、羊和鱼）的未知血清中的这两种酶。这些结果为将来将此类酶应用于生产 ALP 和 AChE 涂层 ELISA 板的研究提供了可能。
{"title":"Isolation, characterization, and ELISA applications of alkaline phosphatase and acetylcholinesterase from Moniezia expansa","authors":"D. A. Darwish, H. Masoud, M. S. Helmy, W. T. Abbas, R. Shaapan, N. Toaleb, M. A. Ibrahim","doi":"10.33899/ijvs.2023.142183.3161","DOIUrl":"https://doi.org/10.33899/ijvs.2023.142183.3161","url":null,"abstract":"Moniezia expansa worms are a significant source of alkaline phosphatase (ALP) and acetylcholinesterase (AChE) enzymes. The current study presents a simple and reproducible ALP and AChE purification method from Moniezia expansa helminthes by precipitating the proteins with ammonium sulfate and chromatography on the Sephacryl S-300 column. The M. expansa ALP purified at 1070.8 U/mg, displaying 6.0 purification folds and 53.6% yield, while M. expansa AChE is at 5250 U/mg, displaying 2.0 purification folds and 43% yield. The M. expansa ALP isoenzyme displayed its optimum activity at pH 9.6, while the M. expansa AChE isoenzyme displayed its optimum activity at pH 8.0. The affinity of M. expansa ALP for several substrates revealed that p-nitrophenyl phosphate preferentially cleaved with a Km value of 4.4 mM. M. expansa AChE preferentially cleaved acetylthiocholine iodide with a Km value of 0.9 mM. M. expansa ALP is strongly stimulated with Co 2+ , Mn 2+ , Ni 2+ , and Mg 2+ and reduced with Zn 2+ , Cu 2+ , Ca 2+ , EDTA and DTT. On the other hand, M. expansa AChE is significantly induced with Co 2+ , Zn 2+ , and Ni 2+ and inhibited with Mg 2+ , Ca 2+ , EDTA, 1,10-phenanthroline and eserine. The antisera of the purified M. expansa ALP and AChE found effective for determining the two enzymes in different unknown sera from different animal species, including humans, sheep and fish. These results may provide a possible future application of such enzymes in producing ALP and AChE-coated ELISA plates for research purposes.","PeriodicalId":14655,"journal":{"name":"Iraqi journal of Veterinary Sciences","volume":"92 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139124995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.33899/ijvs.2023.140352.3039
Enaam E. Jabori, Hana Kh. Ismail, L. W. Khaleel, Ahmed N. Flaih
Abstract
摘要
{"title":"Costus-loaded silver nanoparticles mitigated AMPK and related pathways in the albino rat atherosclerosis model","authors":"Enaam E. Jabori, Hana Kh. Ismail, L. W. Khaleel, Ahmed N. Flaih","doi":"10.33899/ijvs.2023.140352.3039","DOIUrl":"https://doi.org/10.33899/ijvs.2023.140352.3039","url":null,"abstract":"Abstract","PeriodicalId":14655,"journal":{"name":"Iraqi journal of Veterinary Sciences","volume":"86 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139125132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.33899/ijvs.2023.139898.2998
A. G. Atiyah, L. M. Alkattan, A. M. Shareef
Abstract
摘要
{"title":"The radiological study of using fabricated calcium hydroxide from quail eggshell and plasma-rich fibrin for reconstitution of a mandibular bone gap in dogs","authors":"A. G. Atiyah, L. M. Alkattan, A. M. Shareef","doi":"10.33899/ijvs.2023.139898.2998","DOIUrl":"https://doi.org/10.33899/ijvs.2023.139898.2998","url":null,"abstract":"Abstract","PeriodicalId":14655,"journal":{"name":"Iraqi journal of Veterinary Sciences","volume":"33 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139127372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.33899/ijvs.2023.142082.3157
K. I. Alkhazraji, R. A. Naser
Abstract
摘要
{"title":"Macroscopic and histomorphometry investigation of pancreas in adult local partridge (Francolinus francolinus)","authors":"K. I. Alkhazraji, R. A. Naser","doi":"10.33899/ijvs.2023.142082.3157","DOIUrl":"https://doi.org/10.33899/ijvs.2023.142082.3157","url":null,"abstract":"Abstract","PeriodicalId":14655,"journal":{"name":"Iraqi journal of Veterinary Sciences","volume":"44 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139125561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.33899/ijvs.2023.141516.3119
Ahmed S. Naser, Y. Albadrany, Manahel A. Abdullah
Abstract
摘要
{"title":"Evaluation of the advantages of orphenadrine in anaesthesia caused by ketamine in mice","authors":"Ahmed S. Naser, Y. Albadrany, Manahel A. Abdullah","doi":"10.33899/ijvs.2023.141516.3119","DOIUrl":"https://doi.org/10.33899/ijvs.2023.141516.3119","url":null,"abstract":"Abstract","PeriodicalId":14655,"journal":{"name":"Iraqi journal of Veterinary Sciences","volume":"53 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139127800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.33899/ijvs.2023.139817.2984
Fadhaa H. Abdullah, Nagham M. Al-gburi
The dog was an essential source of Salmonella ( S. ) transmission. One hundred sixty-five rectal swabs were taken from apparently healthy dogs 90 and diarrheal dogs 75 in Baghdad province, Iraq. The Salmonella species was found using standard bacterial culture, biochemical tests, an analytical profile index API-20, the VITEK2 compact system, and serotyping. Salmonella isolates were screened using the disc diffusion technique for susceptibility to ten antimicrobials. Findings indicated that the Salmonella species prevalence rate was 6.06% from dogs’ fecal samples, with three species identified; the most common serovar was S. Typhimurium, S . Enteritidis, and S . Muenchen, which was isolated for the first time from dogs in Iraq. Statistically significant risk factors concerning the diarrheal state, breed, feeding, and body condition were found. High occurrences were in diarrheal dogs 10.66% compared to non-diarrheic dogs 2.22%; the large dog breed had a higher isolate rate of Salmonella than the small breed. Thin body condition dogs were more at risk than fatty dogs, and the dogs consuming uncooked feed had a higher percentage of Salmonella isolates and were more at risk than those consuming mixed food. Non-significant differences were found according to the gender, age, and educational status of dog owners. Salmonella isolates exhibited high resistance to cefotaxime, ampicillin, azithromycin, gentamycin, and tetracycline but were sensitive to norfloxacin. All the isolates were multidrug-resistant (MDR) except S . Muenchen. In conclusion, diarrheal and apparently healthy dogs carry and shed resistant Salmonella spp., a potential public health risk.
狗是沙门氏菌（S. ）传播的重要来源。从伊拉克巴格达省 90 只表面健康的狗和 75 只腹泻的狗身上采集了 165 份直肠拭子。通过标准细菌培养、生化试验、API-20 分析概况指数、VITEK2 紧凑型系统和血清分型发现了沙门氏菌的种类。沙门氏菌分离物采用盘扩散技术对 10 种抗菌药进行药敏试验。研究结果表明，狗粪便样本中沙门氏菌的感染率为 6.06%，确定了三个菌种；最常见的血清型是鼠伤寒沙门氏菌、肠炎沙门氏菌和痢疾沙门氏菌。Enteritidis 和 S .Muenchen，这是首次从伊拉克的狗身上分离出的。研究发现，与腹泻状态、品种、喂养和身体状况有关的风险因素在统计学上具有重要意义。腹泻犬的发病率为 10.66%，而非腹泻犬的发病率为 2.22%；大型犬种的沙门氏菌分离率高于小型犬种。体型瘦弱的狗比体型肥胖的狗更容易感染沙门氏菌，食用未煮熟饲料的狗分离出沙门氏菌的比例较高，比食用混合饲料的狗更容易感染沙门氏菌。狗主人的性别、年龄和教育程度差异不大。沙门氏菌分离物对头孢噻肟、氨苄西林、阿奇霉素、庆大霉素和四环素的耐药性较高，但对诺氟沙星敏感。除了 S .Muenchen除外。总之，腹泻狗和表面健康的狗都携带并传播耐药沙门氏菌属，这是一种潜在的公共卫生风险。
{"title":"Risk factors assessment and antimicrobial resistance of Salmonella isolates from apparently healthy and diarrheal dogs in Baghdad, Iraq","authors":"Fadhaa H. Abdullah, Nagham M. Al-gburi","doi":"10.33899/ijvs.2023.139817.2984","DOIUrl":"https://doi.org/10.33899/ijvs.2023.139817.2984","url":null,"abstract":"The dog was an essential source of Salmonella ( S. ) transmission. One hundred sixty-five rectal swabs were taken from apparently healthy dogs 90 and diarrheal dogs 75 in Baghdad province, Iraq. The Salmonella species was found using standard bacterial culture, biochemical tests, an analytical profile index API-20, the VITEK2 compact system, and serotyping. Salmonella isolates were screened using the disc diffusion technique for susceptibility to ten antimicrobials. Findings indicated that the Salmonella species prevalence rate was 6.06% from dogs’ fecal samples, with three species identified; the most common serovar was S. Typhimurium, S . Enteritidis, and S . Muenchen, which was isolated for the first time from dogs in Iraq. Statistically significant risk factors concerning the diarrheal state, breed, feeding, and body condition were found. High occurrences were in diarrheal dogs 10.66% compared to non-diarrheic dogs 2.22%; the large dog breed had a higher isolate rate of Salmonella than the small breed. Thin body condition dogs were more at risk than fatty dogs, and the dogs consuming uncooked feed had a higher percentage of Salmonella isolates and were more at risk than those consuming mixed food. Non-significant differences were found according to the gender, age, and educational status of dog owners. Salmonella isolates exhibited high resistance to cefotaxime, ampicillin, azithromycin, gentamycin, and tetracycline but were sensitive to norfloxacin. All the isolates were multidrug-resistant (MDR) except S . Muenchen. In conclusion, diarrheal and apparently healthy dogs carry and shed resistant Salmonella spp., a potential public health risk.","PeriodicalId":14655,"journal":{"name":"Iraqi journal of Veterinary Sciences","volume":"131 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139128463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.33899/ijvs.2023.141118.3104
Ayman A. Al-Alhially
{"title":"First analysis of beak and feather disease of psittacine in Nineveh province, Iraq","authors":"Ayman A. Al-Alhially","doi":"10.33899/ijvs.2023.141118.3104","DOIUrl":"https://doi.org/10.33899/ijvs.2023.141118.3104","url":null,"abstract":"","PeriodicalId":14655,"journal":{"name":"Iraqi journal of Veterinary Sciences","volume":"33 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139129501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.33899/ijvs.2023.141780.3135
Y. Albadrany, Ahmed S. Naser, Khalid A. Shaban
Abstract
摘要
{"title":"Detection of potential effects of orphenadrine upon anesthesia with propofol and/or thiopental in mice","authors":"Y. Albadrany, Ahmed S. Naser, Khalid A. Shaban","doi":"10.33899/ijvs.2023.141780.3135","DOIUrl":"https://doi.org/10.33899/ijvs.2023.141780.3135","url":null,"abstract":"Abstract","PeriodicalId":14655,"journal":{"name":"Iraqi journal of Veterinary Sciences","volume":"42 17","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139129360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.33899/ijvs.2023.139185.2906
H.Kh. Ismail, H. B. Al-sabaawy, R. T. Altaee, A. Alsaidya, A. H. Alhamdani
Abstract
摘要
{"title":"Study the biochemical, hematological, and histopathological alteration in heat-stress fish treated with an immune stimulant","authors":"H.Kh. Ismail, H. B. Al-sabaawy, R. T. Altaee, A. Alsaidya, A. H. Alhamdani","doi":"10.33899/ijvs.2023.139185.2906","DOIUrl":"https://doi.org/10.33899/ijvs.2023.139185.2906","url":null,"abstract":"Abstract","PeriodicalId":14655,"journal":{"name":"Iraqi journal of Veterinary Sciences","volume":"48 13","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139125221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.33899/ijvs.2023.140242.3033
Sahar O. Saleh, Asmaa A. Hussien, A. Youseef, Waleed K. Younis, A. G. Mubarak
Abstract
摘要
{"title":"Prevalence, antibiotic resistance, and phylogenetic analysis of Listeria monocytogenes isolated from various sources in Egypt: fish, vegetables, and humans","authors":"Sahar O. Saleh, Asmaa A. Hussien, A. Youseef, Waleed K. Younis, A. G. Mubarak","doi":"10.33899/ijvs.2023.140242.3033","DOIUrl":"https://doi.org/10.33899/ijvs.2023.140242.3033","url":null,"abstract":"Abstract","PeriodicalId":14655,"journal":{"name":"Iraqi journal of Veterinary Sciences","volume":"93 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139128798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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