Pub Date : 2024-01-01DOI: 10.33899/ijvs.2023.140808.3091
Firas T. Al-Mubarak, Afnan G. Yaqoub, Manar Alnassar
Abstract
摘要
{"title":"A new RT-PCR assay for the revealing of Newcastle disease viruses by designing a pair of universal primers","authors":"Firas T. Al-Mubarak, Afnan G. Yaqoub, Manar Alnassar","doi":"10.33899/ijvs.2023.140808.3091","DOIUrl":"https://doi.org/10.33899/ijvs.2023.140808.3091","url":null,"abstract":"Abstract","PeriodicalId":14655,"journal":{"name":"Iraqi journal of Veterinary Sciences","volume":"7 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139129157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.33899/ijvs.2023.138795.2842
O. Al-Hyani, Ahmed Kh. Ali, A. Al-Saidya, Hana Kh. Ismail
Abstract
摘要
{"title":"Comparison between two medical drugs' effect on the crushed sciatic nerve healing in dogs","authors":"O. Al-Hyani, Ahmed Kh. Ali, A. Al-Saidya, Hana Kh. Ismail","doi":"10.33899/ijvs.2023.138795.2842","DOIUrl":"https://doi.org/10.33899/ijvs.2023.138795.2842","url":null,"abstract":"Abstract","PeriodicalId":14655,"journal":{"name":"Iraqi journal of Veterinary Sciences","volume":"7 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139129718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.33899/ijvs.2023.141286.3110
S. I. Anwar, S. A. Gharieb
Abstract
摘要
{"title":"Detection of subclinical paratuberculosis in dairy cattle in Egypt","authors":"S. I. Anwar, S. A. Gharieb","doi":"10.33899/ijvs.2023.141286.3110","DOIUrl":"https://doi.org/10.33899/ijvs.2023.141286.3110","url":null,"abstract":"Abstract","PeriodicalId":14655,"journal":{"name":"Iraqi journal of Veterinary Sciences","volume":"5 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139126243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.33899/ijvs.2023.141069.3102
Saifur Rehman, Muhammad H. Khan, Muhammad Bilal, W. P. Lokapirnasari, Shakkeb Ullah, Muhammad Sh. Khan, Muhammad K. Shah, Ambrina Tariq
Abstract
摘要
{"title":"An updated review of human monkeypox disease: A new potential global hazard","authors":"Saifur Rehman, Muhammad H. Khan, Muhammad Bilal, W. P. Lokapirnasari, Shakkeb Ullah, Muhammad Sh. Khan, Muhammad K. Shah, Ambrina Tariq","doi":"10.33899/ijvs.2023.141069.3102","DOIUrl":"https://doi.org/10.33899/ijvs.2023.141069.3102","url":null,"abstract":"Abstract","PeriodicalId":14655,"journal":{"name":"Iraqi journal of Veterinary Sciences","volume":"45 19","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139126996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.33899/ijvs.2023.139935.3000
H. R. Alnajm, Z. K. Imari, Talib A. Al-Rubeaye
The study aimed to research the genetic variation of the Awassi and Naimi sheep breeds using the two genes myostatin (MSTN) and calpastatin (CAST). Blood samples were collected from 100 animals of the two breeds, and then DNA was extracted using a commercial kit. We used the PCR and RFLP techniques to determine genotypes and allele frequencies. The results showed that the MSTN and CAST genes are polymorphic. The MSTN gene has allelic frequencies (M and m) of 0.81, 0.19, and 0.76, 0.24 in the Awassi and Naimi breeds, respectively. The frequencies of the genotypes MM, Mm, and mm in the Awassi breed were 0.70, 0.19, and 0.11, but in the Naimi breed, they were 0.67, 0.13, and 0.20, respectively. Moreover, the number of alleles observed (Na), the effective number of alleles (Ne) and observed (Ho), and expected (He) heterozygosity were found to be 3, 2.30, 0.24, and 0.35 in the Awassi breed and 2, 1.62, 0.17, and 0.26 in the Naimi breed, respectively. The allelic frequencies (M and N) of the CAST of the Awassi and Naimi breeds are 0.86, 0.14, and 0.88, 0.12, respectively. The frequencies of the genotypes MM, MN, and NN in the Awassi breed were 0.94, 0.04, and 0.02, respectively, while for the Naimi breed, they were 0.95, 0.02, and 0.03, respectively. Also, the Na, Ne, Ho, and He were found to be 2.8, 1.72, 29.6, and 28.57 in the Awassi breed and 1.10, 1.23, 0.17, and 0.15 in the Naimi breed, respectively. According to the chi-square of MSTN and CAST genes, both breeds were not in Hardy-Weinberg equilibrium balance.
{"title":"Study of genetic variation of myostatin (MSTN) and calpastatin (CAST) genes in two native Iraqi sheep by PCR-RFLP technique","authors":"H. R. Alnajm, Z. K. Imari, Talib A. Al-Rubeaye","doi":"10.33899/ijvs.2023.139935.3000","DOIUrl":"https://doi.org/10.33899/ijvs.2023.139935.3000","url":null,"abstract":"The study aimed to research the genetic variation of the Awassi and Naimi sheep breeds using the two genes myostatin (MSTN) and calpastatin (CAST). Blood samples were collected from 100 animals of the two breeds, and then DNA was extracted using a commercial kit. We used the PCR and RFLP techniques to determine genotypes and allele frequencies. The results showed that the MSTN and CAST genes are polymorphic. The MSTN gene has allelic frequencies (M and m) of 0.81, 0.19, and 0.76, 0.24 in the Awassi and Naimi breeds, respectively. The frequencies of the genotypes MM, Mm, and mm in the Awassi breed were 0.70, 0.19, and 0.11, but in the Naimi breed, they were 0.67, 0.13, and 0.20, respectively. Moreover, the number of alleles observed (Na), the effective number of alleles (Ne) and observed (Ho), and expected (He) heterozygosity were found to be 3, 2.30, 0.24, and 0.35 in the Awassi breed and 2, 1.62, 0.17, and 0.26 in the Naimi breed, respectively. The allelic frequencies (M and N) of the CAST of the Awassi and Naimi breeds are 0.86, 0.14, and 0.88, 0.12, respectively. The frequencies of the genotypes MM, MN, and NN in the Awassi breed were 0.94, 0.04, and 0.02, respectively, while for the Naimi breed, they were 0.95, 0.02, and 0.03, respectively. Also, the Na, Ne, Ho, and He were found to be 2.8, 1.72, 29.6, and 28.57 in the Awassi breed and 1.10, 1.23, 0.17, and 0.15 in the Naimi breed, respectively. According to the chi-square of MSTN and CAST genes, both breeds were not in Hardy-Weinberg equilibrium balance.","PeriodicalId":14655,"journal":{"name":"Iraqi journal of Veterinary Sciences","volume":"41 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139127015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.33899/ijvs.2023.139379.2932
S. A. Hussein, M. Hamad
Genetic depiction of Mycoplasma isolates was done by a PCR approach from the upper respiratory tract and conjunctival infections in domestic dogs in Mosul City. This was accomplished by amplifying the 16S rRNA gene, which is specific to the Mycoplasma genus. 100 domestic dogs of various ages, sexes, and breeds participated in the study from 1/2/2022 to 1/1/2023. There were 300 swabs total, including conjunctival, nasal, and oropharyngeal swabs. The swabs were subjected to mycoplasmal culturing in suitable conditions. The growing colonies were examined by light and a dissecting microscope. The DNA of growing colonies was extracted and amplified then migrated in agarose gel to observe the bands. The study's findings revealed the isolation of 58 Mycoplasma isolates, with an overall swab isolation rate of around 58% and a high rate of 68% from nasal swabs, then from oropharyngeal swabs and conjunctival swabs 58 and 45% sequentially. PCR technique showed that 34 isolates from the total of 58 isolates were assured for the 16S rRNA gene and yielded a band at 270bp, these findings are regarded as distinctive for the genus Mycoplasma . DNA Sequencing results of the 16S rRNA gene revealed that twenty-three 67.65% out of thirty-four Mycoplasma isolates were indicated as Mycoplasma cynos strain SM-MY-M23 which was dumped in the GenBank nucleotide sequence database under accession number OQ446513, so current results are considered as the first record of Mycoplasma cynos in dogs in Iraq.
{"title":"Genetically diagnosis of Mycoplasma isolated from respiratory and conjunctival infections in household dogs","authors":"S. A. Hussein, M. Hamad","doi":"10.33899/ijvs.2023.139379.2932","DOIUrl":"https://doi.org/10.33899/ijvs.2023.139379.2932","url":null,"abstract":"Genetic depiction of Mycoplasma isolates was done by a PCR approach from the upper respiratory tract and conjunctival infections in domestic dogs in Mosul City. This was accomplished by amplifying the 16S rRNA gene, which is specific to the Mycoplasma genus. 100 domestic dogs of various ages, sexes, and breeds participated in the study from 1/2/2022 to 1/1/2023. There were 300 swabs total, including conjunctival, nasal, and oropharyngeal swabs. The swabs were subjected to mycoplasmal culturing in suitable conditions. The growing colonies were examined by light and a dissecting microscope. The DNA of growing colonies was extracted and amplified then migrated in agarose gel to observe the bands. The study's findings revealed the isolation of 58 Mycoplasma isolates, with an overall swab isolation rate of around 58% and a high rate of 68% from nasal swabs, then from oropharyngeal swabs and conjunctival swabs 58 and 45% sequentially. PCR technique showed that 34 isolates from the total of 58 isolates were assured for the 16S rRNA gene and yielded a band at 270bp, these findings are regarded as distinctive for the genus Mycoplasma . DNA Sequencing results of the 16S rRNA gene revealed that twenty-three 67.65% out of thirty-four Mycoplasma isolates were indicated as Mycoplasma cynos strain SM-MY-M23 which was dumped in the GenBank nucleotide sequence database under accession number OQ446513, so current results are considered as the first record of Mycoplasma cynos in dogs in Iraq.","PeriodicalId":14655,"journal":{"name":"Iraqi journal of Veterinary Sciences","volume":"93 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139128673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.33899/ijvs.2023.134898.2466
Hanaa K. Alshammary, Shaker M. Mirhish
{"title":"Morphological study of laying and non-laying female genital system in peacock (Pavo cristatus)","authors":"Hanaa K. Alshammary, Shaker M. Mirhish","doi":"10.33899/ijvs.2023.134898.2466","DOIUrl":"https://doi.org/10.33899/ijvs.2023.134898.2466","url":null,"abstract":"","PeriodicalId":14655,"journal":{"name":"Iraqi journal of Veterinary Sciences","volume":"49 17","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139126812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.33899/ijvs.2023.140648.3074
Abeer S. Alnakeeb, Qaes T. Alsarhan
This study was targeted to evaluate the infection rate of Anaplasma phagocytophilum in cows in Mosul city-Iraq, using microscopic examination (ME) of the blood smears and nested polymerase chain reaction (N-PCR) technique to evaluate the efficiency of different diagnostic methods used in this study and to determine the parasitemia with the more common leucocytes infected with A. phagocytophilum . 50 blood samples were collected from cows in Mosul city. Blood smears were prepared for the primary detection of inclusion bodies (Morulae) inside leukocytes. The remaining blood was used to confirm the infection using the N-PCR technique. Results revealed that the infection rate of A. phagocytophilum in Mosul City was 58 and 72% using the ME method and N-PCR, respectively. Substantial compatibility was observed between the ME method and N-PCR technique based on the Kappa value 0.699 with a sensitivity of 80.55%, specificity of 100%, and accuracy of 86% of the ME method compared with the N-PCR technique. Infected cows with A. phagocytophilum were suffering from acute disease. Based on the ME of the blood smears, the total parasitemia of A. phagocytophilum inside the leukocytes ranged between 6-29%, with a mean of 17%. The highest significance of parasitemia in neutrophils was 40%, followed by lymphocytes was 25% compared with mononuclear cells, basophils, and eosinophils, which showed no significant change of parasitemia among them. In conclusion, A. phagocytophilum is widespread in cows in Mosul-Iraq; the ME method and N-PCR technique efficiently detect the A. phagocytophilum and neutrophils, which are mostly infected with bacteria.
{"title":"Detection of Anaplasma phagocytophilum in cows in Mosul City, Iraq","authors":"Abeer S. Alnakeeb, Qaes T. Alsarhan","doi":"10.33899/ijvs.2023.140648.3074","DOIUrl":"https://doi.org/10.33899/ijvs.2023.140648.3074","url":null,"abstract":"This study was targeted to evaluate the infection rate of Anaplasma phagocytophilum in cows in Mosul city-Iraq, using microscopic examination (ME) of the blood smears and nested polymerase chain reaction (N-PCR) technique to evaluate the efficiency of different diagnostic methods used in this study and to determine the parasitemia with the more common leucocytes infected with A. phagocytophilum . 50 blood samples were collected from cows in Mosul city. Blood smears were prepared for the primary detection of inclusion bodies (Morulae) inside leukocytes. The remaining blood was used to confirm the infection using the N-PCR technique. Results revealed that the infection rate of A. phagocytophilum in Mosul City was 58 and 72% using the ME method and N-PCR, respectively. Substantial compatibility was observed between the ME method and N-PCR technique based on the Kappa value 0.699 with a sensitivity of 80.55%, specificity of 100%, and accuracy of 86% of the ME method compared with the N-PCR technique. Infected cows with A. phagocytophilum were suffering from acute disease. Based on the ME of the blood smears, the total parasitemia of A. phagocytophilum inside the leukocytes ranged between 6-29%, with a mean of 17%. The highest significance of parasitemia in neutrophils was 40%, followed by lymphocytes was 25% compared with mononuclear cells, basophils, and eosinophils, which showed no significant change of parasitemia among them. In conclusion, A. phagocytophilum is widespread in cows in Mosul-Iraq; the ME method and N-PCR technique efficiently detect the A. phagocytophilum and neutrophils, which are mostly infected with bacteria.","PeriodicalId":14655,"journal":{"name":"Iraqi journal of Veterinary Sciences","volume":"117 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139126152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.33899/ijvs.2023.140335.3040
M. A. Khudeir, A. T. Alsultan, Y. I. Khudhair
Abstract
摘要
{"title":"Novel CRISPR/Cas13- based assay for diagnosis of avian infectious bronchitis","authors":"M. A. Khudeir, A. T. Alsultan, Y. I. Khudhair","doi":"10.33899/ijvs.2023.140335.3040","DOIUrl":"https://doi.org/10.33899/ijvs.2023.140335.3040","url":null,"abstract":"Abstract","PeriodicalId":14655,"journal":{"name":"Iraqi journal of Veterinary Sciences","volume":"8 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139129692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-01-01DOI: 10.33899/ijvs.2022.134545.2378
L. Razzak, Nur Adlin Hisham, Moayad M. Abu Darwish, Hassan Sheikh
Abstract
摘要
{"title":"Efficacy of vaccine from whole killed Vibrio alginolyticus cells on the immune response of white shrimp (Litopenaeus vannamei)","authors":"L. Razzak, Nur Adlin Hisham, Moayad M. Abu Darwish, Hassan Sheikh","doi":"10.33899/ijvs.2022.134545.2378","DOIUrl":"https://doi.org/10.33899/ijvs.2022.134545.2378","url":null,"abstract":"Abstract","PeriodicalId":14655,"journal":{"name":"Iraqi journal of Veterinary Sciences","volume":"32 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139129872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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