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Assessing Prevalence and Antibiotic Resistance of <i>Escherichia coli</i> and Other Enterobacteriaceae Isolated from Cambodian Fermented Fish and Vegetables 评估大肠埃希菌的流行和耐药性从柬埔寨发酵鱼和蔬菜中分离的其他肠杆菌科细菌
4区 农林科学 Q4 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-10-01 DOI: 10.6090/jarq.57.311
Rithy CHRUN, Hasika MITH, Siveng MENG, Sreypov LONG, Pichpunleu BORN, Yasuhiro INATSU
Escherichia coli (E. coli) and other Enterobacteriaceae isolated from fermented products were evaluated for pH, salt tolerance, and multidrug resistance to nine antimicrobial categories, including acetamides, aminoglycosides, β-lactams, cephalosporins, fluoroquinolones, polymyxins, quinolones, sulfonamides, and tetracycline. E. coli was identified in 34% of total fermented samples, followed by Proteus spp. (18%), Providencia spp. (3%), and Citrobacter spp. (3%). Other minor Enterobacteriaceae species detected in 6% of the total samples were Enterobacter aerogenes, Enterobacter cloacae, Ewardsiella tarda, Klebsiella oxytoca, Kluyvera cryocrescens, Morganella moganii, Raoultella planticola, Raoultella terrigena, and Salmonella enterica. Most isolated bacteria can withstand 8% NaCl concentration. However, they were inhibited at 4 pH. Over 50% of the isolated E. coli, Proteus mirabilis, Proteus penneri, and Proteus vulgaris were multidrug-resistant to β-lactams, polypeptides cyclic, sulfonamides, and tetracyclines.
对从发酵产品中分离的大肠杆菌和其他肠杆菌科细菌进行pH值、耐盐性和对9种抗菌药物的耐药性评估,包括乙酰胺类、氨基糖苷类、β-内酰胺类、头孢菌素类、氟喹诺酮类、多粘菌素类、喹诺酮类、磺胺类和四环素类。大肠杆菌占总发酵样品的34%,其次是变形杆菌(18%)、普罗维登西亚(3%)和柠檬酸杆菌(3%)。在6%的总样本中检出的其他小型肠杆菌科菌种为产气肠杆菌、阴沟肠杆菌、迟发爱德华菌、氧化克雷伯菌、低温克卢韦拉菌、莫氏摩根菌、足底拉乌尔菌、terrigera拉乌尔菌和肠沙门氏菌。大多数分离的细菌可以承受8%的NaCl浓度。然而,它们在4 ph下被抑制。超过50%的分离大肠杆菌、奇异变形杆菌、penneri变形杆菌和普罗特斯对β-内酰胺类、多肽环、磺胺类和四环素类具有多重耐药性。
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引用次数: 0
Taper Equations for Evaluating Private Plantation Teak (<i>Tectona grandis</i>) in Thailand 评估泰国私人种植柚木的锥度方程(&lt;i&gt;Tectona grandis&lt;/i&gt;)
4区 农林科学 Q4 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-10-01 DOI: 10.6090/jarq.57.329
Iwao NODA, Woraphun HIMMAPAN, Naoyuki FURUYA, Gaku HITSUMA
We applied data from 407 trees (5-33 years old) from private teak (Tectona grandis) plantations to three typical taper models, including the Goodwin cubic polynomial model comprising hyperbolic and parabolic terms and the Kozak variable-exponent taper model. On the basis of the three models, 18 variants were fitted using nonlinear regression analysis. All models were defined to predict stem diameters overbark using diameter at breast height overbark. A bark thickness equation was prepared to convert overbark diameters to ones excluding the bark. Goodness-of-fit and leave-one-out cross-validation appraisals were used to select the best model. A variant of the Kozak model (Model K8) performed the best across three prediction tests: diameter given height, height given diameter, and log volume given two heights. Taper equation K8, derived from Model K8, provided actual values within the 10% mean error and was sufficiently accurate and precise at the valuable bole part. Teak trees in our study were different in stem form and slender (a high value in slenderness coefficient) compared to those in the state-owned Forest Industry Organization (FIO) teak plantations, and the use of the FIO taper model for slender stems was challenging. Trees in the private plantations generally had thicker barks than those in the FIO plantations. We concluded that equation K8 is recommended for private teak plantations in this study area. These results will contribute to studies on teak taper equations and bark thickness in Thailand.
本文将407棵(5-33岁)柚木人工林的数据应用于三种典型的锥度模型,包括Goodwin三次多项式模型(包含双曲项和抛物线项)和Kozak变指数锥度模型。在这三个模型的基础上,用非线性回归分析方法拟合了18个变量。所有模型都被定义为使用胸围高度上的直径来预测茎的上树皮直径。建立了树皮厚度方程,将上树皮的直径转换为不含树皮的厚度。采用拟合优度评价和留一交叉验证评价来选择最佳模型。Kozak模型的一个变体(模型K8)在三个预测测试中表现最好:给定高度的直径、给定直径的高度和给定两个高度的对数体积。由模型K8导出的锥度方程K8提供了平均误差在10%以内的实际值,在有价值的孔部分足够准确和精确。与国有林工组织(FIO)柚木人工林相比,我们研究的柚木在茎型和细长度(长细系数值较高)方面存在差异,FIO细长茎锥度模型的应用具有挑战性。私人种植园的树木通常比FIO种植园的树木有更厚的树皮。我们的结论是,公式K8是推荐在本研究区域的私人柚木种植园。这些结果将有助于研究泰国柚木的锥度方程和树皮厚度。
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引用次数: 0
Effect of RNA<i>i</i>-mediated Suppression of <i>PGRP-LC</i> Gene Expression on Mortality Rates in Brown Planthoppers (<i>Nilaparvata lugens</i>) Infected with Live Bacteria RNA&lt; i&lt;/i&gt;-介导抑制PGRP-LC&lt;/i&gt;褐飞虱致死率的基因表达(&lt;i&gt;Nilaparvata lugens&lt;/i&gt;)感染活细菌
4区 农林科学 Q4 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-10-01 DOI: 10.6090/jarq.57.281
Aki SAGISAKA, Ritsuko MURAKAMI, Kenji WATANABE, Hiromitsu TANAKA
The brown planthopper (Nilaparvata lugens) is among the most destructive pests of rice in Asia. However, control of this pest is hampered because of the emergence of strains with resistance to standard chemical pesticides. Herein, we investigated the use of RNA interference (RNAi) to knock down genes in the immune system of N. lugens as a possible approach to the control of the pest. We suppressed the expression of the peptidoglycan recognition protein–LC gene and Toll-1 gene by RNAi-mediated knockdown in N. lugens infected with live bacteria. Mortality rates were compared between insects with and without RNAi-mediated gene knockdown. We found that suppression of peptidoglycan recognition protein–LC, which encodes a factor in the immune deficiency pathway, resulted in a significantly increased mortality rate in insects infected with Escherichia coli (gram-negative bacteria) or Micrococcus luteus (gram-positive bacteria). Our results indicate that RNAi knockdown of genes in the immune deficiency pathway will be of value for identifying candidate genes that can be used to control this pest species.
褐飞虱(Nilaparvata lugens)是亚洲最具破坏性的水稻害虫之一。然而,由于出现了对标准化学农药具有抗性的菌株,对这种害虫的控制受到阻碍。在此,我们研究了使用RNA干扰(RNAi)来敲低N. lugens免疫系统中的基因,作为控制害虫的可能方法。我们通过rnai介导的敲低,抑制了lugens感染活菌后肽聚糖识别蛋白- lc基因和Toll-1基因的表达。比较了rnai介导的基因敲低和未敲低昆虫的死亡率。我们发现,抑制肽聚糖识别蛋白- lc(编码免疫缺陷途径中的一个因子)会导致感染大肠杆菌(革兰氏阴性菌)或黄体微球菌(革兰氏阳性菌)的昆虫死亡率显著增加。我们的研究结果表明,RNAi敲低免疫缺陷途径中的基因将对鉴定可用于控制该害虫物种的候选基因具有价值。
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引用次数: 0
Relationship between Gill Raker Number and Early Growth of Pacific Bluefin Tuna <i>Thunnus orientalis</i> 鳃耙数量与太平洋蓝鳍金枪鱼早期生长的关系&lt;i&gt;Thunnus orientalis&lt;/i&gt
4区 农林科学 Q4 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-10-01 DOI: 10.6090/jarq.57.345
Hiroshige TANAKA, Yosuke TANAKA
This study examined the variation in the gill raker number (GRN: a meristic count) of Pacific bluefin tuna Thunnus orientalis in relation to its early growth using otolith microstructure analysis. A total of 360 age-0 fish (fork length: 102 mm-369 mm) in which the gill raker should have been fully developed were analyzed. The GRN on the upper limb, lower limb, and in total (including one gill raker at the joint of two limbs) ranged from 9-13, 21-26, and 32-40, respectively. GRN was negatively related to early growth, as indicated by the mean otolith increment width. Fish with fewer gill rakers attained better growth during the early life stage than fish with more gill rakers. This relationship was especially evident for growth from 15-19 days after hatching soon before metamorphosis. These results suggest that the early-stage growth of bluefin tuna is an important determinant of morphology. Furthermore, GRN can be an alternative tool for monitoring early growth and may also be related to survival and recruitment success.
本研究利用耳石微观结构分析方法,研究了太平洋蓝鳍金枪鱼鳃棘数(GRN:分生计数)的变化与其早期生长的关系。共分析了360条年龄为0岁的鱼(叉长:102 mm-369 mm),其中鳃耙应该已经完全发育。上肢GRN为9-13,下肢GRN为21-26,总GRN为32-40(包括两肢关节处的1个鳃耙)。从平均耳石增量宽度可以看出,GRN与早期生长呈负相关。在生命早期,鳃耙较少的鱼比鳃耙较多的鱼生长得更好。这种关系在孵化后15-19天的生长中尤其明显。这些结果表明,蓝鳍金枪鱼的早期生长是形态的重要决定因素。此外,GRN可以作为监测早期生长的替代工具,也可能与生存和招募成功有关。
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引用次数: 0
Breeding of Waxy Barley Cultivars in the National Barley Breeding Program of Japan 日本国家大麦育种计划中糯大麦品种的选育
4区 农林科学 Q4 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-10-01 DOI: 10.6090/jarq.57.251
Takuji TONOOKA
Waxy barley is considered a good source of dietary fiber and a convenient functional food in Japan; thus, domestic demand is rapidly increasing. However, most of the demand has been met by imports from countries, such as the USA and Canada, as domestic waxy barley production levels have remained low in Japan. To promote the domestic production of waxy barley, the National Agriculture and Food Research Organization has initiated an intensive breeding program to develop and disseminate elite waxy barley cultivars with superior agronomic traits, such as high-yield potential, high disease resistance, and high quality in terms of processing and food. Extensive planting of the newly bred cultivars raised the Japanese domestic self-sufficiency rate of waxy barley from 3 to 49% in 2016 to 2021, respectively.
大麦在日本被认为是膳食纤维的良好来源和方便的功能性食品;因此,国内需求正在迅速增加。然而,由于日本国内的糯大麦生产水平仍然很低,大部分需求都是通过从美国和加拿大等国家进口来满足的。为了促进国内糯大麦的生产,国家农业和食品研究组织启动了一项集约化育种计划,以开发和传播具有优异农艺性状的优质糯大麦品种,如高产潜力、高抗病性、加工和食品质量高。新品种的大量种植使日本国内大麦自给率分别从2016年的3%提高到2021年的49%。
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引用次数: 0
Spatial Sensitivity and Calibration of 10HS Soil Moisture Sensors in Converted Paddy Fields for Practical Use 10HS土壤水分传感器在改造水田的空间灵敏度与标定
4区 农林科学 Q4 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-10-01 DOI: 10.6090/jarq.57.299
Chika ZUKEMURA, Teruhito MIYAMOTO, Hiroshi OSARI
This study confirmed a utility of a 10HS sensor, a capacitance sensor, in converted paddy fields to exploit the practical influence volume from our results and the corrected FSC function. Converted paddy fields in Japan are typically used to cultivate lowland rice or upland crops, and many of them have a problem of poor drainage when upland crops are cultivated. One of the major causes of poor drainage is a presence of a hardpan layer due to soil puddling for lowland rice cultivation. To monitor soil moisture above the hardpan layer without the influence of the hardpan layer, we identified the spatial sensitivity around the sensor and determined the applicability of the sensor with the factory-supplied calibration (FSC) function, including under near-saturated conditions. As a result, regarding the spatial sensitivity, the influence volume of the sensor from our result is much smaller than that reported by Cobos (2008), and we suggested that the sensors should be installed at least 1.5 cm above the boundary between the hardpan and plowed layers. Moreover, regarding the calibrations of the sensors, a corrected FSC function that can account for the bulk density was proposed.
本研究证实了10HS传感器(电容传感器)在改造水田中的实用性,以利用我们的结果和校正的FSC函数的实际影响量。在日本,改造后的稻田通常用于种植低地水稻或旱地作物,其中许多稻田在种植旱地作物时存在排水不畅的问题。排水不良的主要原因之一是由于土壤在低地水稻种植中搅打而形成的硬层。为了在不受硬垫层影响的情况下监测硬垫层以上的土壤湿度,我们确定了传感器周围的空间灵敏度,并利用工厂提供的校准(FSC)函数确定了传感器的适用性,包括在近饱和条件下的适用性。因此,在空间敏感性方面,我们的结果得出的传感器的影响体积比Cobos(2008)报告的要小得多,我们建议传感器应安装在硬耕层和犁耕层之间边界以上至少1.5 cm的位置。此外,对于传感器的校准,提出了一个修正的FSC函数,可以解释堆积密度。
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引用次数: 0
The Role of Single Oral Dose of Excess Vitamin A and/or Vitamin E in Improving Ovarian Function Three Days Post-parturition in Primiparous Dairy Cows 单次口服过量维生素A和/或维生素E对初产奶牛产后3 d卵巢功能的改善作用
4区 农林科学 Q4 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-10-01 DOI: 10.6090/jarq.57.321
Mari AOKI
Dairy farmers dose their cows continuously with high amount of fat-soluble vitamin supplements to improve reproductive capacity and milk quality. However, these amounts are sometimes detrimental to the health of cows. Farmers require effective methods of vitamin supplementation methods without side effects. Our study aims to ascertain the effects of a single, high-concentration oral dose of vitamin A and E supplements on the reproductive performance of dairy cows. The cows were classified based on when did their first ovulation occur: within the first three weeks postpartum (early responder: ER) or after three weeks postpartum (late responder). This is based on the notion that the first postpartum ovulation is a useful indicator of reproductive function recovery in cows. We tested whether supplementation with vitamin A, E, or both effectively induced ovulation during the early postpartum period. Three out of the eight primiparous cows that were not administered a vitamin supplement and were considered ER. All six primiparous cows that were administered a single supplement mixture containing vitamins A (20,000,000 IU) and E (2,000 mg) three days postpartum were ERs. Additionally, all primiparous cows supplied either vitamin A (n = 4) or E (n = 4) alone were also ERs. All primiparous cows treated with even half the amount of vitamins A and E used in Experiment 1 (10,000,000 IU and 1,000 mg, respectively) were designated ERs. These results strongly suggest that even a single dose of excess vitamin A and E (whether administered jointly or separately) can improve ovarian function in dairy cattle during the early postpartum period.
奶农持续给奶牛喂食大量脂溶性维生素补充剂,以提高其繁殖能力和牛奶品质。然而,这些量有时对奶牛的健康有害。农民需要有效的维生素补充方法,没有副作用的方法。本研究旨在确定单次、高浓度口服维生素a和E补充剂对奶牛繁殖性能的影响。根据奶牛首次排卵的时间进行分类:产后前三周内(早期反应者:ER)或产后三周后(晚期反应者)。这是基于产后第一次排卵是奶牛生殖功能恢复的有用指标的概念。我们测试了在产后早期补充维生素A、E或两者是否有效地诱导排卵。8头未服用维生素补充剂的初产奶牛中有3头被认为是ER。产后3天给予含有维生素a(2000万IU)和维生素E (2000 mg)的单一补充混合物的6头初产奶牛均为急诊。此外,所有单独提供维生素A (n = 4)或维生素E (n = 4)的初产奶牛也发生了er。试验1中维生素A和维生素E用量(分别为1000万IU和1000 mg)为一半的所有初产奶牛均被指定为泌乳期。这些结果强烈表明,即使是单剂量的过量维生素a和E(无论是联合给药还是单独给药)也可以改善产后早期奶牛的卵巢功能。
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引用次数: 0
Promotion to Farmers of Snow Compaction (Yuki-Fumi) on Winter Wheat to Control Volunteer Potatoes without Depending on Chemical Materials 向农民推广冬小麦的雪压实(Yuki-Fumi)来控制志愿者马铃薯,而不依赖化学物质
4区 农林科学 Q4 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-10-01 DOI: 10.6090/jarq.57.261
Seiji SHIMODA, Tomoyoshi HIROTA, Masayuki ONODERA
Adaptation to climate change in response to changes in snow cover has led to the development of snow control technology on agricultural fields in Hokkaido. In the control of the soil frost depth and freezing to death of potato tuber leftovers in harvesting during the subsequent cultivation, winter wheat has been an effective countermeasure in crop rotation systems in Hokkaido. We investigated a method used to control soil frost depth by snow compaction (yuki-fumi) to balance between killing potatoes and growing winter wheat. In an experimental test in NARO, we found that snow compaction has a negative effect on wheat growth at a very early stage of the snow cover. Snow compaction does not reduce soil temperatures as much as snow removal, but in potato-to-wheat rotation, it reduced volunteer potatoes by 1/10 because of the shallow position of unharvested potatoes. Snow compaction on wheat can control volunteer potatoes without depending on pesticides and does not adversely affect wheat growth. These environment-friendly agricultural systems have gradually become widespread in eastern Hokkaido.
为了适应气候变化,应对积雪的变化,北海道农业领域的防雪技术得到了发展。冬小麦是北海道轮作制度中控制土壤冻深和马铃薯收获剩余物冻死的有效对策。研究了采用压实法控制土壤霜深的方法,以平衡马铃薯和冬小麦的种植。在NARO的一项试验中,我们发现在积雪覆盖的很早期,积雪压实对小麦生长有负面影响。雪压实并不像除雪那样降低土壤温度,但在马铃薯到小麦的轮作中,由于未收获的马铃薯位置较浅,它减少了1/10的志愿者马铃薯。小麦上的积雪压实可以在不依赖农药的情况下控制志愿马铃薯,也不会对小麦生长产生不利影响。这些环境友好型农业系统在北海道东部逐渐普及。
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引用次数: 0
Conservation Technology Model Fit for Farmlands in a Micro-watershed on the Ethiopian Highlands 适合埃塞俄比亚高原小流域农田的保护技术模式
4区 农林科学 Q4 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-10-01 DOI: 10.6090/jarq.57.269
Kazuhisa KODA, Gebreyohannes GIRMAY, Tesfay BERIHU
The Tigray government in the Federal Democratic Republic of Ethiopia has implemented several water harvesting technologies to improve agriculture in drylands. They constructed 92 micro-dam reservoirs from 1992 to 2012. However, the sedimentation resulting from gully erosion has been settling in more than half of the micro-dam reservoirs. Consequently, it is difficult to provide the required irrigation for the upland fields situated downstream, especially in situations of decreased water availability, caused by the sedimentation over the bottom pipe intake of the irrigational systems/structures. The objectives of the present research are: 1) to verify the conservation of agriculture on farmlands situated at upper slopes for reducing erosion, 2) to estimate the sedimentation rate in micro-dam reservoirs, constructed by the local government, engineers, and residents for recycling the sediments, and 3) to cultivate vegetables on reclaimed farmland by reusing the sediments in micro-dam reservoirs. Thus, this study reports a case study of the water and soil management technology model in a micro-watershed in the Ethiopian highlands.
埃塞俄比亚联邦民主共和国的提格雷政府已经实施了几种集水技术,以改善旱地的农业。从1992年到2012年,他们建造了92座微型水库。然而,超过一半的微坝水库由于沟槽侵蚀而淤积。因此,很难为位于下游的旱地提供所需的灌溉,特别是在灌溉系统/结构的底部管道进水口淤积造成可用水量减少的情况下。本研究的目的是:1)验证坡顶农田的农业保护作用,以减少侵蚀;2)估算由当地政府、工程师和居民为回收沉积物而建造的微坝水库的沉积速率;3)利用微坝水库的沉积物在开垦农田上种植蔬菜。因此,本研究报告了埃塞俄比亚高原小流域水土管理技术模式的案例研究。
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引用次数: 0
Effects of Forage Corn Silage Preparation on Early-instar Larval Survivability and Egg Hatchability of Fall Armyworms (<i>Spodoptera frugiperda</i>) 饲用玉米青贮对秋粘虫(&lt;i&gt;Spodoptera frugiperda&lt;/i&gt;)早期幼虫存活率和卵孵化率的影响
4区 农林科学 Q4 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-10-01 DOI: 10.6090/jarq.57.289
Masayuki HAYASHI, Sachiyo SANADA-MORIMURA, Naoki KATO, Ikuo HATTORI
In this study, we investigated the effects of laboratory-scale silage preparation using a plastic bag and incubation on the early-instar larval viability and egg hatchability of fall armyworms (FAWs) inoculated into corn. In experiments performed to evaluate the effects on larval viability, the impacts of vacuum and anaerobic conditions were assessed as factors expected to influence larval viability after silage preparation. In the experiment that evaluated the effects on egg hatchability, the effects of enclosed fresh corn or fermented corn silage were examined. In the experiment that evaluated the effects of vacuum during silage preparation, no viable larvae were detected in anaerobic conditions, regardless of the length of incubation time (24 h or 14 days). In the experiment that confirmed the effects of carbon dioxide (CO2) gas during silage preparation, no viable larvae were noted under enclosed CO2 conditions, regardless of the incubation period (24 h or 14 days). After 3 days of silage preparation, we also found that egg hatchability was 0.05%. These results suggest that forage corn contaminated with FAW has a reduced larval viability rate and egg hatchability in the early stages of silage preparation, suggesting a low chance of FAW spreading from corn silage prepared under airtight conditions.
本研究研究了实验室规模的袋装青贮和孵育对接种玉米的秋粘虫(FAWs)早期幼虫活力和卵孵化率的影响。在青贮后影响幼虫活力的试验中,真空和厌氧条件作为影响青贮后幼虫活力的因素进行了评估。在评价青贮对鸡蛋孵化率影响的试验中,考察了封闭新鲜玉米和发酵玉米青贮对鸡蛋孵化率的影响。在青贮准备过程中真空影响的试验中,无论孵育时间(24 h或14 d)的长短,在厌氧条件下均未检测到活仔鱼。在证实二氧化碳(CO2)气体在青贮准备过程中的作用的实验中,无论孵育时间是24 h还是14 d,在封闭的CO2条件下都没有发现活仔鱼。青贮准备3 d后,鸡蛋孵化率为0.05%。综上所示,在青贮前期,饲用玉米受FAW污染后,其幼虫存活率和卵孵化率均降低,说明在密闭条件下制备的青贮玉米中FAW的传播几率较低。
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引用次数: 0
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Jarq-japan Agricultural Research Quarterly
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