Pub Date : 2023-07-24DOI: 10.35516/jjps.v16i2.1465
R. Abu Khalaf, A. Nasrallah, G. Albadawi
Cardiovascular diseases, as coronary heart disease, heart failure, and hypertension are the first leading cause of death in the United States and the third globally. CETP is a glycoprotein excreted mainly from the liver and found in plasma. Normal plasma CETP concentration is 1-4 µg/ml, while the ratio increased 70-80% in dyslipidemic patients. There is a growing need for new CETP inhibitors which encourages us to conduct this research. In this work, synthesis and in vitro study for four new 4-bromophenethylbenzamides 9a-d were carried out. In vitro study showed that the targeted compounds 9a-d exhibit acceptable activity against CETP, where compound 9a has a % inhibition of 40.7 at 10 µM concentration. It was found that the presence of the oxy group in both 9a and 9c enhances their activity which could be attributed to Hydrogen-bond formation with the amino acid residues of the CETP binding site.
{"title":"Cholesteryl Ester Transfer Protein Inhibitory Activity of New 4-Bromophenethyl Benzamides","authors":"R. Abu Khalaf, A. Nasrallah, G. Albadawi","doi":"10.35516/jjps.v16i2.1465","DOIUrl":"https://doi.org/10.35516/jjps.v16i2.1465","url":null,"abstract":"Cardiovascular diseases, as coronary heart disease, heart failure, and hypertension are the first leading cause of death in the United States and the third globally. CETP is a glycoprotein excreted mainly from the liver and found in plasma. Normal plasma CETP concentration is 1-4 µg/ml, while the ratio increased 70-80% in dyslipidemic patients. There is a growing need for new CETP inhibitors which encourages us to conduct this research. In this work, synthesis and in vitro study for four new 4-bromophenethylbenzamides 9a-d were carried out. In vitro study showed that the targeted compounds 9a-d exhibit acceptable activity against CETP, where compound 9a has a % inhibition of 40.7 at 10 µM concentration. It was found that the presence of the oxy group in both 9a and 9c enhances their activity which could be attributed to Hydrogen-bond formation with the amino acid residues of the CETP binding site.","PeriodicalId":14719,"journal":{"name":"Jordan Journal of Pharmaceutical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46457752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-24DOI: 10.35516/jjps.v16i2.1474
Aamir Ahmad
Lung cancer is the most common cancer globally, accounting for a quarter of all cancer-related deaths. Non-small cell lung cancer (NSCLC) is the major lung cancer subtype. Research in the last few decades has led to the identification of key molecular targets resulting in targeted therapies, such as the use of tyrosine kinase inhibitors (TKIs) targeting epidermal growth factor receptors (EGFR). EGFR-TKIs themselves have evolved through few generations based on the knowledge gained from gene mutations in the EGFR leading to therapy resistance. As part of pharmacogenetics, it is well known that patients often respond differentially to different therapies, based on their genetic makeup. This has opened up avenues for precision medicine in the treatment of NSCLC patients with the identification of EGFR mutations and the most optimum treatment strategy. Since time is of the essence, it is critical that the NSCLC patients be administered a therapy that they are most likely to respond to. Evolving data validates this notion and it is expected that such an approach will invariably lead to improved patient outcomes.
{"title":"Precision Medicine and Pharmacogenetics: Stratification and Improved Outcome in Non-Small Cell Lung Cancer","authors":"Aamir Ahmad","doi":"10.35516/jjps.v16i2.1474","DOIUrl":"https://doi.org/10.35516/jjps.v16i2.1474","url":null,"abstract":"Lung cancer is the most common cancer globally, accounting for a quarter of all cancer-related deaths. Non-small cell lung cancer (NSCLC) is the major lung cancer subtype. Research in the last few decades has led to the identification of key molecular targets resulting in targeted therapies, such as the use of tyrosine kinase inhibitors (TKIs) targeting epidermal growth factor receptors (EGFR). EGFR-TKIs themselves have evolved through few generations based on the knowledge gained from gene mutations in the EGFR leading to therapy resistance. As part of pharmacogenetics, it is well known that patients often respond differentially to different therapies, based on their genetic makeup. This has opened up avenues for precision medicine in the treatment of NSCLC patients with the identification of EGFR mutations and the most optimum treatment strategy. Since time is of the essence, it is critical that the NSCLC patients be administered a therapy that they are most likely to respond to. Evolving data validates this notion and it is expected that such an approach will invariably lead to improved patient outcomes.","PeriodicalId":14719,"journal":{"name":"Jordan Journal of Pharmaceutical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42870181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-24DOI: 10.35516/jjps.v16i2.1484
Muhammed Alzweiri
A new evaluation method based on gas chromatography (GC) is considered for the screening of the inhibitory activity of various compounds against certain enzymes using immobilized pH-sensitive matrix pellets. This approach of analysis is being utilized in developing sensitive GC methods for enzymes attributed to pH change in their media. Particularly, for those which require accurate and sensitive assay methods. The method has been implemented in drug discovery research related to antagonism of carbonic anhydrase, isozyme 3 (CAIII). This isozyme has a strong potential in treating obesity, hyperlipidemia and cancer. The method was also used in investigation of various peptide derivatives with an epoxide warhead for their anti-lipase activity. Recently, the method has been used in evaluating some tacrine analogues for their inhibition against acetylcholinesterase enzyme. The latter is important in treating Alzheimer’s disease (AD) which is a worldwide mental disorder manifested with dementia symptoms and anticipated to be the second causality of deaths by 2040.
{"title":"Gas Chromatography with Modified pH‐sensitive Pellets in Evaluating the Activity of Some Enzymes Attributed with a Change in the Medium pH","authors":"Muhammed Alzweiri","doi":"10.35516/jjps.v16i2.1484","DOIUrl":"https://doi.org/10.35516/jjps.v16i2.1484","url":null,"abstract":"A new evaluation method based on gas chromatography (GC) is considered for the screening of the inhibitory activity of various compounds against certain enzymes using immobilized pH-sensitive matrix pellets. This approach of analysis is being utilized in developing sensitive GC methods for enzymes attributed to pH change in their media. Particularly, for those which require accurate and sensitive assay methods. The method has been implemented in drug discovery research related to antagonism of carbonic anhydrase, isozyme 3 (CAIII). This isozyme has a strong potential in treating obesity, hyperlipidemia and cancer. The method was also used in investigation of various peptide derivatives with an epoxide warhead for their anti-lipase activity. Recently, the method has been used in evaluating some tacrine analogues for their inhibition against acetylcholinesterase enzyme. The latter is important in treating Alzheimer’s disease (AD) which is a worldwide mental disorder manifested with dementia symptoms and anticipated to be the second causality of deaths by 2040.","PeriodicalId":14719,"journal":{"name":"Jordan Journal of Pharmaceutical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43255932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.35516/jjps.v16i2.1322
Hamza Alhamad, D. Jaber, Mohammad B. Nusair, Fares Albahar, S. Edaily, N. Q. Al-hamad, Haneen A Basheer
Introduction: This study evaluates undergraduate pharmacy students' and examiners’ perceptions of implementing OSCE exam. Methods: A sample of 185 undergraduate pharmacy students (138 from Zarqa University and 47 from Yarmouk University) and 20 examiners were invited to complete a quantitative survey and qualitative focus group discussion, respectively. Results: 103 out of 185 (56%, response rate) undergraduate pharmacy students completed the quantitative survey, with 11 examiners out of 20 (55%) agreeing to participate in the examiners' focus group discussion. Most pharmacy students agreed that OSCE exam was a practical and useful experience (74.8%) and should be part of the assessment in other pharmacy courses (61.2%). However, less than a quarter thought that OSCE exam was not fair (17.5%), very intimidating (20.4%), and needed more time (29.1%). Examiners were generally in favour of OSCE exam being well-organised and well-administered despite the need for a large place to conduct and a good number of pharmacy staff to implement. Conclusion: Pharmacy students and examiners agreed that OSCE exam is an excellent and preferable clinical assessment tool. This study provides a scheme to evaluate OSCE exam as a clinical assessment tool and would help policy-makers gain more insight into the impact of implementing OSCE exam on students' clinical knowledge and communicational skills development and learning process.
{"title":"Implementing OSCE Exam for Undergraduate Pharmacy Students: A Two Institutional Mixed-Method Study","authors":"Hamza Alhamad, D. Jaber, Mohammad B. Nusair, Fares Albahar, S. Edaily, N. Q. Al-hamad, Haneen A Basheer","doi":"10.35516/jjps.v16i2.1322","DOIUrl":"https://doi.org/10.35516/jjps.v16i2.1322","url":null,"abstract":"Introduction: This study evaluates undergraduate pharmacy students' and examiners’ perceptions of implementing OSCE exam. \u0000Methods: A sample of 185 undergraduate pharmacy students (138 from Zarqa University and 47 from Yarmouk University) and 20 examiners were invited to complete a quantitative survey and qualitative focus group discussion, respectively. \u0000Results: 103 out of 185 (56%, response rate) undergraduate pharmacy students completed the quantitative survey, with 11 examiners out of 20 (55%) agreeing to participate in the examiners' focus group discussion. Most pharmacy students agreed that OSCE exam was a practical and useful experience (74.8%) and should be part of the assessment in other pharmacy courses (61.2%). However, less than a quarter thought that OSCE exam was not fair (17.5%), very intimidating (20.4%), and needed more time (29.1%). Examiners were generally in favour of OSCE exam being well-organised and well-administered despite the need for a large place to conduct and a good number of pharmacy staff to implement. \u0000Conclusion: Pharmacy students and examiners agreed that OSCE exam is an excellent and preferable clinical assessment tool. This study provides a scheme to evaluate OSCE exam as a clinical assessment tool and would help policy-makers gain more insight into the impact of implementing OSCE exam on students' clinical knowledge and communicational skills development and learning process.","PeriodicalId":14719,"journal":{"name":"Jordan Journal of Pharmaceutical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47364485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This research was carried out to assess the minimum inhibitory concentration (MIC) and the genotoxic potential of ethanolic and aqueous (cold and hot) fruit extracts of Capparis spinosa L. (C. spinosa) plant against different types of bacterial strains. The antimicrobial effect of these extracts against the tested bacteria was investigated using broth microdilution method. The potential genotoxic effect was evaluated by ERIC-PCR technique. Results of the current study revealed that the MIC values of ethanolic fruit extract against the tested bacterial had a range of 12.5 mg/ml to 25 mg/ml. However, aqueous fruit extracts had an MIC with a range of 50 mg/ml to 100 mg/mL. The potential genotoxic activity of cold aqueous extract was determined according to the changes in ERIC-PCR profile of E. coli strain treated with extract in comparison to that untreated (negative control). Results of this study suggest the genotoxic effect of aqueous fruit extract on E. coli. Further research is required to assess and identify the biological molecules and their mechanisms in the context of the genotoxicity. In vivo genotoxicity assessment or with the presence of liver extract is recommended to evaluate the safety of using fruits for therapeutic purposes and a valuable nutrient source.
{"title":"In vitro Assessment of Antibacterial Activity and Potential Genotoxic Effect of Fruit Extracts of Capparis spinosa L. Plant","authors":"G. Adwan","doi":"10.35516/jjps.v16i2.517","DOIUrl":"https://doi.org/10.35516/jjps.v16i2.517","url":null,"abstract":"This research was carried out to assess the minimum inhibitory concentration (MIC) and the genotoxic potential of ethanolic and aqueous (cold and hot) fruit extracts of Capparis spinosa L. (C. spinosa) plant against different types of bacterial strains. The antimicrobial effect of these extracts against the tested bacteria was investigated using broth microdilution method. The potential genotoxic effect was evaluated by ERIC-PCR technique. Results of the current study revealed that the MIC values of ethanolic fruit extract against the tested bacterial had a range of 12.5 mg/ml to 25 mg/ml. However, aqueous fruit extracts had an MIC with a range of 50 mg/ml to 100 mg/mL. The potential genotoxic activity of cold aqueous extract was determined according to the changes in ERIC-PCR profile of E. coli strain treated with extract in comparison to that untreated (negative control). Results of this study suggest the genotoxic effect of aqueous fruit extract on E. coli. Further research is required to assess and identify the biological molecules and their mechanisms in the context of the genotoxicity. In vivo genotoxicity assessment or with the presence of liver extract is recommended to evaluate the safety of using fruits for therapeutic purposes and a valuable nutrient source.","PeriodicalId":14719,"journal":{"name":"Jordan Journal of Pharmaceutical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46325413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.35516/jjps.v16i2.1330
F. Brahmi, Nabyla Khaled-Khodja, Radia Bezeghouche, Soraya Bouharis, M. F. Elsebai, Khodir Madani, L. Boulekbache‐Makhlouf
This study was carried out to safeguard ancestral knowledge about most Lamiaceae plants used by the population of the Bejaia region (north-eastern Algeria) for medicinal and food purposes. Using 200 questionnaire sheets, ethnobotanical surveys were performed between February and July 2020. The data were analyzed by calculating quantitative indices such as Relative Citation Frequency (RFC), Plant Part Value (PPV) use index, and Fidelity Level (FL). It was shown that women hold ethnobotanical information (52%) more than men (48%), older persons are expected to provide more reliable information and the majority of users have a university level. Otherwise, herbal medicine is used more in rural areas than in urban and 55% of the studied plants are cultivated while 45% are wild. The leaves are the most used part (PPV = 0.592) and the infusion method was the most commonly used (69.7%). Ethnobotanical analysis revealed that Mentha spicata L. (RFC=0.44), Lavendula stoechas L. (RFC=0.215), and Salvia officinalis L. (RFC=0.205) are frequently used. Digestive pathologies are the major therapeutic indications and 41.44% of species were used for seasoning meat and fish. This survey could constitute an important source of information and a database for further research in the fields of phytochemistry and pharmacology
开展这项研究是为了保护贝加亚地区(阿尔及利亚东北部)人口用于医药和食品目的的大多数Lamiaceae植物的祖先知识。在2020年2月至7月期间，使用200份问卷进行了民族植物学调查。通过计算相对被引频次(RFC)、植物成分价值(PPV)利用指数和保真度(FL)等定量指标对数据进行分析。研究表明，女性拥有的民族植物学信息(52%)比男性(48%)多，老年人有望提供更可靠的信息，大多数用户具有大学学历。此外，草药在农村地区的使用比在城市更多，研究的植物中55%是栽培的，45%是野生的。使用最多的部位是叶片(PPV = 0.592)，使用最多的部位是冲泡法(69.7%)。民族植物学分析结果显示，薄荷(Mentha spicata L.) (RFC=0.44)、薰衣草(Lavendula stoechas L.) (RFC=0.215)和鼠尾草(Salvia officinalis L.) (RFC=0.205)被广泛使用。消化疾病是主要的治疗适应症，41.44%的品种用于调味肉类和鱼类。该调查可为植物化学和药理学领域的进一步研究提供重要的信息来源和数据库
{"title":"Ethnobotanical Study of the Most Lamiaceae Used as Medicinal and Culinary Plants by the Population of Bejaia Province, Algeria","authors":"F. Brahmi, Nabyla Khaled-Khodja, Radia Bezeghouche, Soraya Bouharis, M. F. Elsebai, Khodir Madani, L. Boulekbache‐Makhlouf","doi":"10.35516/jjps.v16i2.1330","DOIUrl":"https://doi.org/10.35516/jjps.v16i2.1330","url":null,"abstract":"This study was carried out to safeguard ancestral knowledge about most Lamiaceae plants used by the population of the Bejaia region (north-eastern Algeria) for medicinal and food purposes. Using 200 questionnaire sheets, ethnobotanical surveys were performed between February and July 2020. The data were analyzed by calculating quantitative indices such as Relative Citation Frequency (RFC), Plant Part Value (PPV) use index, and Fidelity Level (FL). It was shown that women hold ethnobotanical information (52%) more than men (48%), older persons are expected to provide more reliable information and the majority of users have a university level. Otherwise, herbal medicine is used more in rural areas than in urban and 55% of the studied plants are cultivated while 45% are wild. The leaves are the most used part (PPV = 0.592) and the infusion method was the most commonly used (69.7%). Ethnobotanical analysis revealed that Mentha spicata L. (RFC=0.44), Lavendula stoechas L. (RFC=0.215), and Salvia officinalis L. (RFC=0.205) are frequently used. Digestive pathologies are the major therapeutic indications and 41.44% of species were used for seasoning meat and fish. This survey could constitute an important source of information and a database for further research in the fields of phytochemistry and pharmacology","PeriodicalId":14719,"journal":{"name":"Jordan Journal of Pharmaceutical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43993532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ebtesam Alhawamdeh, N. Bulatova, A. Yousef, M. Alabbadi, E. A. Omer
Vitiligo is brought on by functional melanocyte loss and manifests as white maculae that may cover the whole body's skin. There is a genetic background in the pathogenesis of vitiligo. Polymorphisms in different parts of catalase gene may affect the disease activity and result in less functional catalase, thus, accumulation of hydrogen peroxide, one of the oxidative factors that damage melanocytes. We evaluated the CAT 262 genetic polymorphism of vitiligo patients using the polymerase chain reaction (PCR) technique with at least one C and at least one T model. The study included 48 vitiligo patient and 51 control individuals. Family history of vitiligo was present in 27.1% of patients and autoimmune disease were diagnosed in 16.7% of patients. Three quarters of vitiligo patients (75.0%) reported that emotional stress was the major triggering factor for their disease. The CC genotype was predominant (56.2% in vitiligo patients and 62.7% in control) with no significant difference between the study groups (p=0.7). Catalase activity in blood was comparable between the study arms (159.1±21.6 MU/L in vitiligo patients and 151.3±25.4 MU/L in controls (p=0.15). We conclude that neither genetic polymorphism in CAT 262 C/T nor blood catalase activity is associated with vitiligo.
{"title":"A Cross-sectional Study of the Catalase Genetic Polymorphism (-262 cytosine/ thymine) and Blood Catalase Activity among Jordanian Vitiligo Patients","authors":"Ebtesam Alhawamdeh, N. Bulatova, A. Yousef, M. Alabbadi, E. A. Omer","doi":"10.35516/jjps.v16i2.438","DOIUrl":"https://doi.org/10.35516/jjps.v16i2.438","url":null,"abstract":"Vitiligo is brought on by functional melanocyte loss and manifests as white maculae that may cover the whole body's skin. There is a genetic background in the pathogenesis of vitiligo. Polymorphisms in different parts of catalase gene may affect the disease activity and result in less functional catalase, thus, accumulation of hydrogen peroxide, one of the oxidative factors that damage melanocytes. We evaluated the CAT 262 genetic polymorphism of vitiligo patients using the polymerase chain reaction (PCR) technique with at least one C and at least one T model. The study included 48 vitiligo patient and 51 control individuals. Family history of vitiligo was present in 27.1% of patients and autoimmune disease were diagnosed in 16.7% of patients. Three quarters of vitiligo patients (75.0%) reported that emotional stress was the major triggering factor for their disease. The CC genotype was predominant (56.2% in vitiligo patients and 62.7% in control) with no significant difference between the study groups (p=0.7). Catalase activity in blood was comparable between the study arms (159.1±21.6 MU/L in vitiligo patients and 151.3±25.4 MU/L in controls (p=0.15). We conclude that neither genetic polymorphism in CAT 262 C/T nor blood catalase activity is associated with vitiligo.","PeriodicalId":14719,"journal":{"name":"Jordan Journal of Pharmaceutical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41683403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.35516/jjps.v16i2.1319
Naoual Teggar, B. Bakchiche, Mohamed El-Sayed Abdel-Aziz, S. Bardaweel, M. Ghareeb
Propolis is considered a natural resin produced by the bee and is still used in folk medicine. Six propolis samples from Apis mellifera (P1-P6) collected from different regions in Algeria were investigated for their contents and biological activities. The obtained results revealed that propolis P1 exhibited the highest total phenolics (210.93 mg GAE/g propolis), total flavonoids (34.33 mg QE/g propolis), and tannins (23.36 mg CE/g propolis). For antioxidant activities, P1 showed strong free radical scavenging activity with EC50 values of 0.055, 0.0306, 0.109 and 0.071 mg/mL, respectively for DPPH, ABTS, FRAP, and phosphomolybdenum assays. On the other hand, all propolis demonstrated antibacterial activities against G+ve bacteria (S. aureus) with slightly higher activities that were associated with P1 and P5 (9.83 and 10.92mm, respectively). P5 exhibited the lowest MIC and MBC against S. aureus with values of 62.5 and 125 µg/ml, respectively. Furthermore, all propolis had moderate to low antimicrobial activities against C. albicans (yeast) with moderate activities for P1 and P6 (13.33 and 8.50 mm, respectively). Chemical profiling of the most bioactive propolis samples (P1, P4, and P5) using HPLC‑fingerprint analysis mainly led to detecting phenolic acids and flavonoids in variable percentages.
{"title":"Chemical Composition and Biological Evaluation of Algerian Propolis from Six Different Regions","authors":"Naoual Teggar, B. Bakchiche, Mohamed El-Sayed Abdel-Aziz, S. Bardaweel, M. Ghareeb","doi":"10.35516/jjps.v16i2.1319","DOIUrl":"https://doi.org/10.35516/jjps.v16i2.1319","url":null,"abstract":"Propolis is considered a natural resin produced by the bee and is still used in folk medicine. Six propolis samples from Apis mellifera (P1-P6) collected from different regions in Algeria were investigated for their contents and biological activities. The obtained results revealed that propolis P1 exhibited the highest total phenolics (210.93 mg GAE/g propolis), total flavonoids (34.33 mg QE/g propolis), and tannins (23.36 mg CE/g propolis). For antioxidant activities, P1 showed strong free radical scavenging activity with EC50 values of 0.055, 0.0306, 0.109 and 0.071 mg/mL, respectively for DPPH, ABTS, FRAP, and phosphomolybdenum assays. On the other hand, all propolis demonstrated antibacterial activities against G+ve bacteria (S. aureus) with slightly higher activities that were associated with P1 and P5 (9.83 and 10.92mm, respectively). P5 exhibited the lowest MIC and MBC against S. aureus with values of 62.5 and 125 µg/ml, respectively. Furthermore, all propolis had moderate to low antimicrobial activities against C. albicans (yeast) with moderate activities for P1 and P6 (13.33 and 8.50 mm, respectively). Chemical profiling of the most bioactive propolis samples (P1, P4, and P5) using HPLC‑fingerprint analysis mainly led to detecting phenolic acids and flavonoids in variable percentages.","PeriodicalId":14719,"journal":{"name":"Jordan Journal of Pharmaceutical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41667339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.35516/jjps.v16i2.1331
Boshra Yosef, N. Kaddar, Arige Boubou
Type 2 diabetes mellitus (T2DM) is an increasingly prevalent chronic disease that associates with an increased risk of micro-and macrovascular complications. There is persuasive evidence that dapagliflozin may reduce chronic inflammation besides its glucose-lowering effect, which in term prevents the development of the disease and its complications. Therefore, this study aims to evaluate the effects of dapagliflozin on the inflammatory marker C-reactive protein (CRP) levels in T2DM patients. Patients with T2DM were randomly assigned into two groups, group 1 (n=52) receiving a daily dose of dapagliflozin as an add-on therapy with oral antihyperglycemic agents, and group 2 (control, n=60) who received oral antihyperglycemic agents (Metformin, Sulfonylureas, Thiazolidinediones, and Gliptins). After six months, our results showed a significant change in CRP levels from baseline after receiving dapagliflozin compared to the control. Although the reduction level of CRP was statically significant with both 5 mg and 10 mg doses, it was higher with the latter one. In addition, the reduction in CRP levels was statistically significant in both controlled and uncontrolled, but more important in uncontrolled disease. An insignificant positive correlation was seen between HbA1c and CRP on admission (r: 0.21, p: 0.1) and during the follow-up period, at 3 months (r: 0.10, p: 0.4) and 6 months (r: 0.08, p: 0.5). Our study showed that dapagliflozin has a beneficial effect on inflammation by reducing CRP levels CRP in patients with T2DM.
{"title":"Evaluation of the Effect of Dapagliflozin on CRP Levels in Type 2 Diabetes Patients","authors":"Boshra Yosef, N. Kaddar, Arige Boubou","doi":"10.35516/jjps.v16i2.1331","DOIUrl":"https://doi.org/10.35516/jjps.v16i2.1331","url":null,"abstract":"Type 2 diabetes mellitus (T2DM) is an increasingly prevalent chronic disease that associates with an increased risk of micro-and macrovascular complications. There is persuasive evidence that dapagliflozin may reduce chronic inflammation besides its glucose-lowering effect, which in term prevents the development of the disease and its complications. Therefore, this study aims to evaluate the effects of dapagliflozin on the inflammatory marker C-reactive protein (CRP) levels in T2DM patients. Patients with T2DM were randomly assigned into two groups, group 1 (n=52) receiving a daily dose of dapagliflozin as an add-on therapy with oral antihyperglycemic agents, and group 2 (control, n=60) who received oral antihyperglycemic agents (Metformin, Sulfonylureas, Thiazolidinediones, and Gliptins). After six months, our results showed a significant change in CRP levels from baseline after receiving dapagliflozin compared to the control. Although the reduction level of CRP was statically significant with both 5 mg and 10 mg doses, it was higher with the latter one. In addition, the reduction in CRP levels was statistically significant in both controlled and uncontrolled, but more important in uncontrolled disease. An insignificant positive correlation was seen between HbA1c and CRP on admission (r: 0.21, p: 0.1) and during the follow-up period, at 3 months (r: 0.10, p: 0.4) and 6 months (r: 0.08, p: 0.5). Our study showed that dapagliflozin has a beneficial effect on inflammation by reducing CRP levels CRP in patients with T2DM.","PeriodicalId":14719,"journal":{"name":"Jordan Journal of Pharmaceutical Sciences","volume":"73 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41272143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.35516/jjps.v16i2.1321
Y. R. Yabansabra, Sally Felter Bebari, E. Simaremare
Dengue hemorrhagic fever (DHF) is a disease caused by infection with the dengue virus. It is controlled by using repellents that protect humans from mosquito bites. One of the repellents used by the community includes DEET chemicals but natural repellents used against mosquitoes such as the zodia plant are also needed. Zodia leaves (Evodia suaveolens Scheff) contain linalool and -pinene compounds as well as evodiamine and rutacarpine. Linalool functions by disrupting the nervous system in mosquitoes thereby causing convulsion and death. Therefore, this study aims to formulate and evaluate the zodia oil and test its effectiveness as a mosquito repellent (Aedes aegypti L) with repelling power method. The formulations were prepared with various concentrations of 25 %, 50 %, and 75 %. Based on the results, the protective power of Formula I (25 %) for 0, 1st, 2nd, and 3rd hours, respectively, was 100%, 100 %, 89.28 %, and 92.85 %. Furthermore, Formula II (50 % concentration) showed a protective power of 100 %, 100 %, 90.90 % and 91.66 %, while the third Formula (75 % concentration) showed a protection power of 100 %, 100 %, 96.15% and 93.33%. Therefore, it was concluded that the three zodia oil formulas are effective as a repellent against Aedes aegypti L mosquitoes.
{"title":"The Effectiveness of Zodia Leaves (Evodia Suaveolens Scheff) Oil as Aedes aegypti L Mosquito Repellent in Papua","authors":"Y. R. Yabansabra, Sally Felter Bebari, E. Simaremare","doi":"10.35516/jjps.v16i2.1321","DOIUrl":"https://doi.org/10.35516/jjps.v16i2.1321","url":null,"abstract":"Dengue hemorrhagic fever (DHF) is a disease caused by infection with the dengue virus. It is controlled by using repellents that protect humans from mosquito bites. One of the repellents used by the community includes DEET chemicals but natural repellents used against mosquitoes such as the zodia plant are also needed. Zodia leaves (Evodia suaveolens Scheff) contain linalool and -pinene compounds as well as evodiamine and rutacarpine. Linalool functions by disrupting the nervous system in mosquitoes thereby causing convulsion and death. Therefore, this study aims to formulate and evaluate the zodia oil and test its effectiveness as a mosquito repellent (Aedes aegypti L) with repelling power method. The formulations were prepared with various concentrations of 25 %, 50 %, and 75 %. Based on the results, the protective power of Formula I (25 %) for 0, 1st, 2nd, and 3rd hours, respectively, was 100%, 100 %, 89.28 %, and 92.85 %. Furthermore, Formula II (50 % concentration) showed a protective power of 100 %, 100 %, 90.90 % and 91.66 %, while the third Formula (75 % concentration) showed a protection power of 100 %, 100 %, 96.15% and 93.33%. Therefore, it was concluded that the three zodia oil formulas are effective as a repellent against Aedes aegypti L mosquitoes.","PeriodicalId":14719,"journal":{"name":"Jordan Journal of Pharmaceutical Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47700420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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