Pub Date : 2018-06-10DOI: 10.29252/JORJANIBIOMEDJ.6.2.21
Z. Mohammadi, Shamsozoha Abolmaali, A. Akbarzadeh
Preparing Nanoarchaeosome Containing Triptorelin Acetate and Evaluation of Its Cellular Toxic Effect on PC3 Prostate Cancer Cell Line Zeynab Haj Mohammadi, Shamsozoha Abolmaali, Azim Akbarzadeh 1. Faculty of Biotechnology Department for Microbial biotechnology, Semnan University, Semnan, Iran. 2. Faculty of Biotechnology Department for Microbial biotechnology, Semnan University, Semnan, Iran. (0000-00016342-0828) 3. Pasteur Institute of Iran, Tehran, Iran.
{"title":"Preparing Nanoarchaeosome Containing Triptorelin Acetate and Evaluation of Its Cellular Toxic Effect on PC3 Prostate Cancer Cell Line","authors":"Z. Mohammadi, Shamsozoha Abolmaali, A. Akbarzadeh","doi":"10.29252/JORJANIBIOMEDJ.6.2.21","DOIUrl":"https://doi.org/10.29252/JORJANIBIOMEDJ.6.2.21","url":null,"abstract":"Preparing Nanoarchaeosome Containing Triptorelin Acetate and Evaluation of Its Cellular Toxic Effect on PC3 Prostate Cancer Cell Line Zeynab Haj Mohammadi, Shamsozoha Abolmaali, Azim Akbarzadeh 1. Faculty of Biotechnology Department for Microbial biotechnology, Semnan University, Semnan, Iran. 2. Faculty of Biotechnology Department for Microbial biotechnology, Semnan University, Semnan, Iran. (0000-00016342-0828) 3. Pasteur Institute of Iran, Tehran, Iran.","PeriodicalId":14723,"journal":{"name":"Jorjani Biomedicine Journal","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76275786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-01DOI: 10.29252/JORJANIBIOMEDJ.6.1.1
Soraya Larki, M. Maleki
Background and objectives: Endometrial tissue growth and its activity outside the uterus cause endometriosis. It has been suggested that various epigenetic deviations play a major role in the pathogenesis of endometriosis. Steroidogenic factor 1 (SF-1; NR5A1) is an essential transcription factor for estrogen biosynthesis in endometrial cells. The expression of SF-1 in endometriosis and lack of expression in normal endometrium is primarily determined by its promoter methylation. Here, we aimed to compare the methylation status of the SF-1 gene promoter region in women with endometriosis in comparison to healthy subjects. Methods: In the present case–control study, DNA was extracted from 25 endometrial tissue samples from women with endometriosis and 5 normal post-hysterectomy endometrium tissues which were collected from Tabriz hospitals including Vali-e-Asr, Taleghani, 29 Bahman and Shams in 2016. The obtained DNA samples were subjected to Bisulfite-treatment. Finally, the status of SF1gene promoter methylation was evaluated by methylation specific PCR method. Statistical analyses including descriptive and inferential statistics were conducted using tables, bar charts by statistical software SPSS version 20 and independence test. Results: The methylation status of SF-1 gene promoter was decreased significantly in endometriosis samples (P<0.05). Conclusion: SF-1 gene promoter hypomethylation could increase the relative expression of SF-1 gene in endometriosis which may lead to the development or progression of the disease.
背景与目的:子宫内膜组织生长及其在子宫外的活动可引起子宫内膜异位症。各种表观遗传偏差在子宫内膜异位症的发病机制中起重要作用。甾体生成因子1 (SF-1;NR5A1)是子宫内膜细胞雌激素生物合成的重要转录因子。SF-1在子宫内膜异位症中的表达和在正常子宫内膜中的表达缺失主要是由其启动子甲基化决定的。在这里，我们的目的是比较SF-1基因启动子区域的甲基化状态在子宫内膜异位症妇女与健康受试者的比较。方法:选取2016年在大不里士(Tabriz) Vali-e-Asr、Taleghani、29 Bahman和Shams等医院采集的子宫内膜异位症患者子宫内膜样本25份和子宫切除术后正常子宫内膜样本5份，进行病例对照研究。得到的DNA样品进行亚硫酸氢盐处理。最后，采用甲基化特异性PCR方法评价sf1基因启动子甲基化状态。统计分析包括描述性统计和推断性统计，采用SPSS version 20统计软件，采用表格、柱状图进行统计分析，并进行独立性检验。结果:SF-1基因启动子甲基化状态在子宫内膜异位症中显著降低(P<0.05)。结论:SF-1基因启动子低甲基化可增加SF-1基因在子宫内膜异位症中的相对表达，可能导致疾病的发生或进展。
{"title":"DNA hypomethylation of the nuclear receptor subfamily 5 (NR5A1) gene promoter is associated with endometriosis among women in north west of Iran","authors":"Soraya Larki, M. Maleki","doi":"10.29252/JORJANIBIOMEDJ.6.1.1","DOIUrl":"https://doi.org/10.29252/JORJANIBIOMEDJ.6.1.1","url":null,"abstract":"Background and objectives: Endometrial tissue growth and its activity outside the uterus cause endometriosis. It has been suggested that various epigenetic deviations play a major role in the pathogenesis of endometriosis. Steroidogenic factor 1 (SF-1; NR5A1) is an essential transcription factor for estrogen biosynthesis in endometrial cells. The expression of SF-1 in endometriosis and lack of expression in normal endometrium is primarily determined by its promoter methylation. Here, we aimed to compare the methylation status of the SF-1 gene promoter region in women with endometriosis in comparison to healthy subjects. Methods: In the present case–control study, DNA was extracted from 25 endometrial tissue samples from women with endometriosis and 5 normal post-hysterectomy endometrium tissues which were collected from Tabriz hospitals including Vali-e-Asr, Taleghani, 29 Bahman and Shams in 2016. The obtained DNA samples were subjected to Bisulfite-treatment. Finally, the status of SF1gene promoter methylation was evaluated by methylation specific PCR method. Statistical analyses including descriptive and inferential statistics were conducted using tables, bar charts by statistical software SPSS version 20 and independence test. Results: The methylation status of SF-1 gene promoter was decreased significantly in endometriosis samples (P<0.05). Conclusion: SF-1 gene promoter hypomethylation could increase the relative expression of SF-1 gene in endometriosis which may lead to the development or progression of the disease.","PeriodicalId":14723,"journal":{"name":"Jorjani Biomedicine Journal","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80701239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-01DOI: 10.29252/JORJANIBIOMEDJ.6.1.44
Maedeh Sadat Alavi, Sheida Jabal Ameli
Background and objectives: Cognitive-behavioral therapy is one of the interventions used in the treatment of chronic medical conditions such as MS in recent years. Hence, this study was designed to determine the effectiveness of cognitive-behavioral therapy on emotional control of MS patients in the city of Isfahan. Methods: The present study was a semi-experimental research which was conducted by using a pretest-posttest design with the control group. The study population consisted of all members of the MS Society of Isfahan in 2016-2017. Using the available sampling method, 30 subjects were selected and randomly divided into two experimental and control groups (15 subjects for each group). Both groups completed Williams and Chambless emotional control questionnaire as the pretest. After eight sessions (two one-hour sessions per week for four weeks) of cognitive-behavioral therapy, the posttest was performed for the groups. The data were analyzed using descriptive statistical methods, including mean and standard deviation and inferential statistics including univariate covariance analysis in the SPSS 20 software. Results: The study participants included 16 married women and 14 married men. The mean age of the participants in the experimental and control groups was 30.23 ± 7.06 and 29.34 ± 7.15, respectively. The results of the univariate covariance analysis showed that a significant difference in the mean scores of emotional control from the pre-test to the post-test stage between the experimental group and the control group (P = 0.001). Thus, in the experimental group, the mean total score of emotional control in the pretest and post-test phases was equal to 71.66 and 31.33, respectively, while these values in the control group in the pre-test and post-test phases were 67.06 and 62.93, respectively. Also in the experimental group, the scores of four sub-scales of anger, depressed mood, anxiety, and positive emotions revealed significant differences between the posttest stage and the pre-test stage (P =0.001). Therefore, the cognitive-behavioral therapy was effective in improving the emotional control of MS patients. Conclusion: Considering the effectiveness of cognitive-behavioral therapy, we recommended the use of this therapeutic method to improve the emotional control of the patients with MS.
{"title":"The Effectiveness of Cognitive-Behavioral Therapy on Emotional Control of MS Patients in the City of Isfahan","authors":"Maedeh Sadat Alavi, Sheida Jabal Ameli","doi":"10.29252/JORJANIBIOMEDJ.6.1.44","DOIUrl":"https://doi.org/10.29252/JORJANIBIOMEDJ.6.1.44","url":null,"abstract":"Background and objectives: Cognitive-behavioral therapy is one of the interventions used in the treatment of chronic medical conditions such as MS in recent years. Hence, this study was designed to determine the effectiveness of cognitive-behavioral therapy on emotional control of MS patients in the city of Isfahan. Methods: The present study was a semi-experimental research which was conducted by using a pretest-posttest design with the control group. The study population consisted of all members of the MS Society of Isfahan in 2016-2017. Using the available sampling method, 30 subjects were selected and randomly divided into two experimental and control groups (15 subjects for each group). Both groups completed Williams and Chambless emotional control questionnaire as the pretest. After eight sessions (two one-hour sessions per week for four weeks) of cognitive-behavioral therapy, the posttest was performed for the groups. The data were analyzed using descriptive statistical methods, including mean and standard deviation and inferential statistics including univariate covariance analysis in the SPSS 20 software. Results: The study participants included 16 married women and 14 married men. The mean age of the participants in the experimental and control groups was 30.23 ± 7.06 and 29.34 ± 7.15, respectively. The results of the univariate covariance analysis showed that a significant difference in the mean scores of emotional control from the pre-test to the post-test stage between the experimental group and the control group (P = 0.001). Thus, in the experimental group, the mean total score of emotional control in the pretest and post-test phases was equal to 71.66 and 31.33, respectively, while these values in the control group in the pre-test and post-test phases were 67.06 and 62.93, respectively. Also in the experimental group, the scores of four sub-scales of anger, depressed mood, anxiety, and positive emotions revealed significant differences between the posttest stage and the pre-test stage (P =0.001). Therefore, the cognitive-behavioral therapy was effective in improving the emotional control of MS patients. Conclusion: Considering the effectiveness of cognitive-behavioral therapy, we recommended the use of this therapeutic method to improve the emotional control of the patients with MS.","PeriodicalId":14723,"journal":{"name":"Jorjani Biomedicine Journal","volume":"72 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85591263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-01DOI: 10.29252/JORJANIBIOMEDJ.6.1.22
M. Tajbakhsh, N. Soleimani
Background and objectives: Historically, herbs and plants have been used for their therapeutic properties in the form of flavors and preservatives. Recently, the application of medicinal herbs has increased considering their numerous benefits and minimum side-effects. Treatment of bacterial infections is currently a major challenge in the healthcare systems across the world. The present study aimed to assess the bacterial effects of Zingiber officinale, Aloysia citrodora and Artemisia dracunculus essential oils on the survival of standard Gram-positive and Gram-negative bacterial strains. Methods: In the present experimental study, we evaluated the effects of Tarragon (A. dracunculus), Ginger (Z. officinale) and Lemon Beebrush (A. citrodora) essential oils on 6 strains of Staphylococcus aureus, Klebsiella spp, Salmonella typhimurium, Staphylococcus epidermidis, Proteus spp and Corynebacterium diphtheriae. The well-diffusion method was applied to assess the antibacterial properties of the essential oils. Moreover, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) tests were used to determine the bacterial and inhibitory concentrations of the extracts. Results: MIC and MBC results demonstrated that the ginger extract (0.125 mg/mL) had the most significant impact on Staphylococcus aureus, Salmonella typhimurium, and Staphylococcus epidermidis. Furthermore, tarragon extract (0.03125 mg/mL) had the most significant effect on Staphylococcus aureus, Proteus spp, and Corynebacterium diphtheriae. The antibacterial effects of these essential oils were not observed on other bacteria. Conclusion: Medicinal plants have long been used for their therapeutic properties. According to the results, ginger and tarragon extracts are effective combinations for the treatment of the infections caused by Gram-negative and Gram-positive bacteria.
{"title":"Evaluation of the Bactericidal Effects of Zingiber officinale, Aloysia citrodora and Artemisia dracunculus on the Survival of Standard Gram-Positive and Gram-Negative Bacterial Strains","authors":"M. Tajbakhsh, N. Soleimani","doi":"10.29252/JORJANIBIOMEDJ.6.1.22","DOIUrl":"https://doi.org/10.29252/JORJANIBIOMEDJ.6.1.22","url":null,"abstract":"Background and objectives: Historically, herbs and plants have been used for their therapeutic properties in the form of flavors and preservatives. Recently, the application of medicinal herbs has increased considering their numerous benefits and minimum side-effects. Treatment of bacterial infections is currently a major challenge in the healthcare systems across the world. The present study aimed to assess the bacterial effects of Zingiber officinale, Aloysia citrodora and Artemisia dracunculus essential oils on the survival of standard Gram-positive and Gram-negative bacterial strains. Methods: In the present experimental study, we evaluated the effects of Tarragon (A. dracunculus), Ginger (Z. officinale) and Lemon Beebrush (A. citrodora) essential oils on 6 strains of Staphylococcus aureus, Klebsiella spp, Salmonella typhimurium, Staphylococcus epidermidis, Proteus spp and Corynebacterium diphtheriae. The well-diffusion method was applied to assess the antibacterial properties of the essential oils. Moreover, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) tests were used to determine the bacterial and inhibitory concentrations of the extracts. Results: MIC and MBC results demonstrated that the ginger extract (0.125 mg/mL) had the most significant impact on Staphylococcus aureus, Salmonella typhimurium, and Staphylococcus epidermidis. Furthermore, tarragon extract (0.03125 mg/mL) had the most significant effect on Staphylococcus aureus, Proteus spp, and Corynebacterium diphtheriae. The antibacterial effects of these essential oils were not observed on other bacteria. Conclusion: Medicinal plants have long been used for their therapeutic properties. According to the results, ginger and tarragon extracts are effective combinations for the treatment of the infections caused by Gram-negative and Gram-positive bacteria.","PeriodicalId":14723,"journal":{"name":"Jorjani Biomedicine Journal","volume":"45 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72631438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-01DOI: 10.29252/JORJANIBIOMEDJ.6.1.12
B. Yahyaei, M. Nouri, Hamid Matmir
Background and objectives: Anabolic steroids are routinely consumed by athletes. Therefore, this study was conducted to investigate the effect of Boldenone (BOL) on testicular tissue and the healing effect of jujube (Ziziphus jujuba) hydroalcoholic extract in addition to physical exercise. Methods: In the present case–control study, 42 male wistar rats were purchased and divided into 3 experimental groups. 28 rats were subjected to BOL injection and subsequently divided into 4 groups. The control group, Sham and 8-week BOL group which was also divided into 4 subsequent groups of controlling BOL complications after 8 weeks, untreated control, jujube extract and jujube extract along with physical exercise. Results: In the present study we found that jujube extract exerted healing effects on all groups pf treated rats in addition to the exercise training groups. Conclusion: Jujube extract along with physical exercise may exert healing effects on testicular tissue after administration of anabolic BOL steroid.
{"title":"Healing effects of Ziziphus jujuba hydroalcoholic extract with exercise training on histopathological changes of male wistar rats testicular tissue in response to Boldenone steroid administration","authors":"B. Yahyaei, M. Nouri, Hamid Matmir","doi":"10.29252/JORJANIBIOMEDJ.6.1.12","DOIUrl":"https://doi.org/10.29252/JORJANIBIOMEDJ.6.1.12","url":null,"abstract":"Background and objectives: Anabolic steroids are routinely consumed by athletes. Therefore, this study was conducted to investigate the effect of Boldenone (BOL) on testicular tissue and the healing effect of jujube (Ziziphus jujuba) hydroalcoholic extract in addition to physical exercise. Methods: In the present case–control study, 42 male wistar rats were purchased and divided into 3 experimental groups. 28 rats were subjected to BOL injection and subsequently divided into 4 groups. The control group, Sham and 8-week BOL group which was also divided into 4 subsequent groups of controlling BOL complications after 8 weeks, untreated control, jujube extract and jujube extract along with physical exercise. Results: In the present study we found that jujube extract exerted healing effects on all groups pf treated rats in addition to the exercise training groups. Conclusion: Jujube extract along with physical exercise may exert healing effects on testicular tissue after administration of anabolic BOL steroid.","PeriodicalId":14723,"journal":{"name":"Jorjani Biomedicine Journal","volume":"72 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86330859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-06-01DOI: 10.29252/JORJANIBIOMEDJ.6.1.33
D. Bagheri, R. Mohseni, S. Mahdavi
Background and objectives: Environmental pollution is a major cause of various diseases. Massive production of hospital, industrial and household wastes lead to several health issues, threatening community health on a daily basis. The present study aimed to determine the association between socioeconomic status and pro-environmental behaviors in the citizens of Gorgan, Iran. Methods: This cross-sectional, descriptive-analytical study was conducted on 400 citizens of Gorgan. Participants were divided into three regions based on urban categorization. Data were collected using demographic and socioeconomic questionnaires. To assess environmental behaviors, a standard questionnaire was used based on the Dunlap spectrum. Data analysis was performed in SPSS version 16 using the analysis of variance (ANOVA), independent samples t-test and factor analysis. Results: No positive significant correlation was observed between gender and environmental behaviors. The results of ANOVA showed a significant association between marital status and environmental behaviors. In addition, the results of factor analysis indicated that five factors explained 55.49% of environmental behaviors. The results of ANOVA also demonstrated that middle-class citizens had a more responsible attitude toward the environment compared to others (P<0.05). Conclusion: According to the results, socioeconomic status influenced the social value orientations and responsible behaviors of individuals toward the environment. Therefore, increasing the quality of life and providing comprehensive education could enhance pro-environmental behaviors and promote community health.
背景与目的:环境污染是导致多种疾病的主要原因。医院、工业和家庭废物的大量产生导致若干健康问题，每天威胁着社区的健康。本研究旨在确定社会经济地位与伊朗戈尔根市民的亲环境行为之间的关系。方法:本横断面描述性分析研究对戈尔根市400名市民进行。根据城市分类，参与者被分为三个区域。通过人口统计和社会经济问卷收集数据。为了评估环境行为，使用了基于邓拉普谱的标准问卷。使用SPSS version 16进行数据分析，采用方差分析(ANOVA)、独立样本t检验和因子分析。结果:性别与环境行为无显著正相关。方差分析结果显示，婚姻状况与环境行为之间存在显著相关性。此外，因子分析结果表明，五个因素解释了55.49%的环境行为。方差分析的结果还显示，中产阶级公民对环境的负责态度比其他公民更强(P<0.05)。结论:社会经济地位影响着个体的社会价值取向和环境责任行为。因此，提高生活质量和提供全面的教育可以增强亲环境行为和促进社区健康。
{"title":"Association of Socioeconomic Status and Pro-Environmental Behaviors in the Citizens of Gorgan, Iran (2017)","authors":"D. Bagheri, R. Mohseni, S. Mahdavi","doi":"10.29252/JORJANIBIOMEDJ.6.1.33","DOIUrl":"https://doi.org/10.29252/JORJANIBIOMEDJ.6.1.33","url":null,"abstract":"Background and objectives: Environmental pollution is a major cause of various diseases. Massive production of hospital, industrial and household wastes lead to several health issues, threatening community health on a daily basis. The present study aimed to determine the association between socioeconomic status and pro-environmental behaviors in the citizens of Gorgan, Iran. Methods: This cross-sectional, descriptive-analytical study was conducted on 400 citizens of Gorgan. Participants were divided into three regions based on urban categorization. Data were collected using demographic and socioeconomic questionnaires. To assess environmental behaviors, a standard questionnaire was used based on the Dunlap spectrum. Data analysis was performed in SPSS version 16 using the analysis of variance (ANOVA), independent samples t-test and factor analysis. Results: No positive significant correlation was observed between gender and environmental behaviors. The results of ANOVA showed a significant association between marital status and environmental behaviors. In addition, the results of factor analysis indicated that five factors explained 55.49% of environmental behaviors. The results of ANOVA also demonstrated that middle-class citizens had a more responsible attitude toward the environment compared to others (P<0.05). Conclusion: According to the results, socioeconomic status influenced the social value orientations and responsible behaviors of individuals toward the environment. Therefore, increasing the quality of life and providing comprehensive education could enhance pro-environmental behaviors and promote community health.","PeriodicalId":14723,"journal":{"name":"Jorjani Biomedicine Journal","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74960007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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