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The mycobiome affects viral susceptibility of skin epithelium 霉菌生物群影响皮肤上皮细胞对病毒的敏感性
IF 2.2 4区 医学 Q3 MYCOLOGY Pub Date : 2024-07-09 DOI: 10.1016/j.mycmed.2024.101499
Liam F. Peterson , Lisa A. Beck , Matthew G. Brewer
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引用次数: 0
Exploring the Co-occurrence of dermatophyte infection and ichthyosis: A report of 3 cases 探讨皮癣菌感染与鱼鳞病的并发症:3 个病例的报告。
IF 2.2 4区 医学 Q3 MYCOLOGY Pub Date : 2024-07-07 DOI: 10.1016/j.mycmed.2024.101498
Elisabetta Magnaterra, Elisa M. Difonzo, Manfredi Magliulo, Samantha F. Berti, Massimo Gola, Luigi Pisano

Dermatophyte infections frequently pose diagnostic challenges, especially when occurring alongside ichthyosis, a genetic skin disorder characterized by dry, thickened, scaly skin. This case series outlines three cases where dermatophyte infections overlapped with ichthyosis, emphasizing the complexities in clinical identification and differential diagnosis. Atypical clinical presentations in these cases led to initial misdiagnoses. Ichthyosis, a genetic skin disorder characterized by thickened and scaly skin, creates an environment conducive to dermatophyte settlement, complicating the diagnostic process. The cases highlight the importance of considering fungal infections, even when clinical features deviate from the expected course. A vigilant diagnostic approach, including mycological examinations, is crucial for accurate identification and timely management.

皮癣菌感染经常给诊断带来挑战,尤其是与鱼鳞病同时发生时。鱼鳞病是一种遗传性皮肤病,其特征是皮肤干燥、增厚、脱屑。本系列病例概述了三个皮癣菌感染与鱼鳞病重叠的病例,强调了临床识别和鉴别诊断的复杂性。这些病例的非典型临床表现导致了最初的误诊。鱼鳞病是一种遗传性皮肤病,其特点是皮肤增厚、鳞屑增多,这为皮癣菌的定居创造了有利环境,使诊断过程变得更加复杂。这些病例强调了考虑真菌感染的重要性,即使临床特征与预期病程有偏差。警惕的诊断方法,包括真菌学检查,对于准确识别和及时治疗至关重要。
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引用次数: 0
Invasive phaeohyphomycosis co-infection with Alternaria spp. and Curvularia spp. in a neutropenic host 一名中性粒细胞减少的宿主同时感染交替孢霉属和卷曲孢霉属的侵袭性真菌病
IF 2.2 4区 医学 Q3 MYCOLOGY Pub Date : 2024-07-02 DOI: 10.1016/j.mycmed.2024.101497
Anna Sikora , Jeffrey Johnson , Ana Velez , John Greene

Phaeohyphomycoses are infections caused by dark-walled dematiaceous fungi. Alternaria and Curvularia are two genera of dematiaceous molds known to cause invasive fungal rhinosinusitis, particularly in immunocompromised patients. Co-infection with two dematiaceous fungi is rarely reported in the literature. This report describes a case of biopsy proven invasive fungal rhinosinusitis with Alternaria spp. and Curvularia spp. co-infection in a neutropenic host. The infection characteristics, microbiologic findings, and treatment are described.

真菌性鼻炎是由黑壁脱霉真菌引起的感染。Alternaria和Curvularia是两种已知可引起侵袭性真菌性鼻炎的脱霉菌属,尤其是在免疫力低下的患者中。文献中很少报道两种脱霉真菌同时感染的病例。本报告描述了一例经活检证实的侵袭性真菌性鼻炎病例,患者为中性粒细胞减少的宿主,同时伴有交替孢属真菌和卷曲孢属真菌的双重感染。文中描述了感染特征、微生物学检查结果和治疗方法。
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引用次数: 0
Asymptomatic colonization of stray dogs and domestic cats with Trichophyton mentagrophytes II* in Northern Iran 伊朗北部流浪狗和家猫身上的无症状毛癣菌 II*。
IF 2.2 4区 医学 Q3 MYCOLOGY Pub Date : 2024-06-26 DOI: 10.1016/j.mycmed.2024.101496
Vahid Oladzad , Ayatollah Nasrollahi Omran , Iman Haghani , Mojtaba Nabili , Jacques Guillot , Seyedmojtaba Seyedmousavi , Mohammad Taghi Hedayati

Background

Despite changes in the epidemiology of dermatophyte infections, the incidence of fungal infections associated with Trichophyton species still remains high among dogs and cats. The objective of the present study was to isolate and characterize dermatophytes from dogs and cats in Iran.

Method:

From December 2022 to May 2023, skin and hair samples were collected from symptomatic and asymptomatic cats and dogs in Mazandaran, a northern province of Iran. The samples were then inoculated into Mycosel™ Agar. Dermatophyte isolates were identified by sequencing the internal transcribed spacer region. Antifungal susceptibility tests were conducted using the Clinical and Laboratory Standards Institute (CLSI-M38-A3).

Result

Of the 250 samples collected (from 200 dogs and 50 cats), 20 (from 19 dogs and one cat) (8.0 %) were positive for dermatophyte growth. Based on sequence and phylogenetic analysis, all isolates belonged to T. mentagrophytes II*. Of these positive samples, 14 (70.0 %), 3 (15.0 %), 2 (10.0 %), and 1 (2.0 %) were isolated from asymptomatic stray dogs, symptomatic stray dogs, symptomatic domestic dogs, and symptomatic cats, respectively. Luliconazole and terbinafine displayed potent activity against all T. mentagrophytes isolates, with Minimum inhibitory concentration (MIC) values of 0.016 µg/ml. Miconazole and griseofulvin demonstrated higher MIC (1 and 8 µg/ml).

Conclusion

The present study indicated that T. mentagrophytes II* asymptomatic carriage is frequent in stray dogs in Iran. The potential risk to public health needs to be evaluated However, T. mentagrophytes genotype VIII, considered as an endemic and emerging human pathogenic clone in several countries, was not detected during the present survey.

背景:尽管皮癣菌感染的流行病学发生了变化,但猫狗中毛癣菌属真菌感染的发病率仍然很高。本研究旨在从伊朗的狗和猫身上分离出皮真菌并确定其特征:方法:2022 年 12 月至 2023 年 5 月,在伊朗北部省份马赞达兰收集了有症状和无症状猫狗的皮肤和毛发样本。然后将样本接种到 Mycosel™ 琼脂中。通过对内部转录间隔区进行测序,对分离出的皮真菌进行鉴定。抗真菌药敏试验采用临床和实验室标准研究所(CLSI-M38-A3)进行:在收集的 250 份样本(来自 200 只狗和 50 只猫)中,有 20 份(来自 19 只狗和 1 只猫)(8.0%)皮真菌生长呈阳性。根据序列和系统发育分析,所有分离株都属于颚癣毛囊菌 II*。在这些阳性样本中,14 个(70.0 %)、3 个(15.0 %)、2 个(10.0 %)和 1 个(2.0 %)分别从无症状的流浪狗、有症状的流浪狗、有症状的家犬和有症状的猫中分离出来。卢利康唑和特比萘芬对所有分离出的脑膜炎嗜血杆菌都有很强的抑制作用,最低抑制浓度(MIC)值为 0.016 µg/ml。咪康唑和格列齐特的最低抑菌浓度(MIC)较高(1 µg/ml 和 8 µg/ml):本研究表明,T. mentagrophytes II* 无症状携带在伊朗的流浪狗中很常见。然而,在本次调查中并未检测到在多个国家被视为地方病和新出现的人类致病克隆的T. mentagrophytes基因型VIII。
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引用次数: 0
Easy-to-use imaging-cytometry assay to analyze chitin patterns in yeasts 简便易用的成像-计数测定法,用于分析酵母菌中的几丁质模式。
IF 2.2 4区 医学 Q3 MYCOLOGY Pub Date : 2024-06-21 DOI: 10.1016/j.mycmed.2024.101493
Delphine Aldebert , Bastien Suarez , François Bettega , Emilie Boucher , Cecile Garnaud , Muriel Cornet

Background & Aim

Pathogenic fungi are a major threat to public health, and fungal infections are becoming increasingly common and treatment resistant. Chitin, a component of the fungal cell wall, modifies host immunity and contributes to antifungal resistance. Moreover, chitin content is regulated by chitin synthases and chitinases. However, the specific roles and mechanisms remain unclear. In this study, we developed a cytometric imaging assay to quantify chitin content and identify the distribution of chitin in the yeast cell wall.

Methods

The Candida albicans SC5314 and Nakaseomyces glabratus (ex. C. glabrata) ATCC2001 reference strains, as well as 106 clinical isolates, were used. Chitin content, distribution, and morphological parameters were analysed in 12 yeast species. Moreover, machine learning statistical software was used to evaluate the ability of the cytometric imaging assay to predict yeast species using the values obtained for these parameters.

Results

Our imaging-cytometry assay was repeatable, reproducible, and sensitive to variations in chitin content in C. albicans mutants or after antifungal stimulation. The evaluated parameters classified the yeast species into the correct clade with an accuracy of 85 %.

Conclusion

Our findings demonstrate that this easy-to-use assay is an effective tool for the exploration of chitin content in yeast species.

背景与目的:致病真菌是公共卫生的主要威胁,真菌感染越来越常见,耐药性也越来越强。几丁质是真菌细胞壁的一种成分,它能改变宿主的免疫力并导致抗真菌性。此外,几丁质含量受几丁质合成酶和几丁质酶的调节。然而,其具体作用和机制仍不清楚。在本研究中,我们开发了一种细胞成像检测方法,用于量化几丁质含量并确定几丁质在酵母细胞壁中的分布:方法:使用白念珠菌 SC5314 和 Nakaseomyces glabratus (ex. C. glabrata) ATCC2001 参考菌株以及 106 株临床分离株。分析了 12 种酵母菌的几丁质含量、分布和形态参数。此外,还使用机器学习统计软件评估了细胞成像测定法利用这些参数值预测酵母菌种类的能力:结果:我们的成像-细胞计量测定法具有可重复性和再现性,而且对白茨酵母突变体或抗真菌刺激后几丁质含量的变化很敏感。所评估的参数将酵母菌物种归入正确支系的准确率为 85%:我们的研究结果表明,这种易于使用的检测方法是检测酵母菌几丁质含量的有效工具。
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引用次数: 0
Increased terbinafine resistance among clinical genotypes of Trichophyton mentagrophytes/T. interdigitale species complex harboring squalene epoxidase gene mutations 携带角鲨烯环氧化物酶基因突变的脑门毛癣菌/趾间毛癣菌复合体临床基因型对特比萘芬的耐药性增强
IF 3.6 4区 医学 Q3 MYCOLOGY Pub Date : 2024-06-16 DOI: 10.1016/j.mycmed.2024.101495
Leila Zahedi Mohammadi , Masoomeh Shams-Ghahfarokhi , Zahra Salehi , Mehdi Razzaghi-Abyaneh

Terbinafine resistance has become epidemic as an emerging problem in treatment of dermatohpytosis. This could be attributed in part to a point mutation in the squalene epoxidase (SQLE) gene. In this study, point mutations in the SQLE gene were studied in T. rubrum and T. mentagrophytes/T. interdigitale species complex as two main causative agents of dermatophytosis. Antifungal susceptibility of clinical isolates of T. rubrum (n = 27) and T. mentagrophytes/T. interdigitale (n = 56) was assessed using the M38–3rd edition CLSI method. The SQLE gene and ITS region were sequenced for all the fungal strains, and the mutation sites and genotypes of the terbinafine-resistant strains were characterized. The results demonstrated that, in T. rubrum, the minimum inhibitory concentration of terbinafine (MIC50 and MIC90) was 0.03 μg/ml, and the geometric mean (G mean) concentration was 0.02. For the T. mentagrophytes complex, the MIC50 and MIC90 were 0.03 and 1.0 μg/ml, respectively, and the G mean concentration was 0.04 μg/ml. Four out of the five resistant strains were T. indotineae harboring the F397L and Q408L mutations, while the last one was T. mentagrophytes genotype VII, which harbors the F397L mutation. T. indotineae was the prominent causative agent of terbinafine resistance, with 80 % of the isolates, and T. mentagrophytes genotype VII was introduced as a new genotype in the terbinafine-resistant T. mentagrophytes complex. Our findings further substantiate the importance of antifungal susceptibility testing in selecting the choice of drug for effective treatment of dermatophytosis and highlight the importance of screening dermatophyte species for point mutations responsible for newly developed resistant strains to improve the current knowledge of overcoming infections caused by resistant species.

特比萘芬耐药性已成为治疗皮癣的一个新问题。这可能部分归因于角鲨烯环氧化酶(SQLE)基因的点突变。在这项研究中,研究了作为皮肤癣菌病两种主要致病菌的红癣菌(T. rubrum)和脑癣菌(T. mentagrophytes/T.interigitale)复合菌种中 SQLE 基因的点突变。采用 M38-3rd edition CLSI 方法评估了临床分离的 T. rubrum(n = 27)和 T. mentagrophytes/T. interdigitale(n = 56)的抗真菌敏感性。对所有真菌菌株的 SQLE 基因和 ITS 区域进行了测序,并对特比萘芬耐药菌株的突变位点和基因型进行了鉴定。结果表明,特比萘芬对红念珠菌的最小抑菌浓度(MIC50 和 MIC90)为 0.03 μg/ml,几何平均浓度(G 平均值)为 0.02。对于曼陀罗菌复合体,MIC50 和 MIC90 分别为 0.03 和 1.0 微克/毫升,几何平均浓度为 0.04 微克/毫升。五株耐药菌株中有四株是携带 F397L 和 Q408L 突变的吲哚癣菌,最后一株是携带 F397L 突变的颚癣菌基因型 VII。T.indotineae是对特比萘芬耐药的主要致病菌,占分离株的80%,而T.mentagrophytes基因型VII是对特比萘芬耐药的T.mentagrophytes复合体中的一个新基因型。我们的研究结果进一步证实了抗真菌药敏试验在选择药物以有效治疗皮癣菌病方面的重要性,并强调了筛查皮癣菌物种中导致新产生耐药菌株的点突变的重要性,以提高目前克服耐药物种引起的感染的知识。
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引用次数: 0
Antifungal efficacy and immunomodulatory effect of PLGA nanoparticle-encapsulated itraconazole in histoplasmosis in vivo model PLGA纳米粒包裹的伊曲康唑在组织胞浆菌病体内模型中的抗真菌疗效和免疫调节作用
IF 2.2 4区 医学 Q3 MYCOLOGY Pub Date : 2024-06-12 DOI: 10.1016/j.mycmed.2024.101494
Susana P. Mejía , Daniela López , Luz Elena Cano , Julián D. Muñoz , Jahir Orozco , Tonny W. Naranjo

Introduction

Histoplasma capsulatum is the etiological agent of histoplasmosis, the most common endemic pulmonary mycosis. Itraconazole (ITZ) is the choice for mild disease and a step-down therapy in severe and disseminated clinical presentations. Drug encapsulation into nanoparticles (NPs) is an alternative to improve drug solubility and bioavailability, reducing undesirable interactions and drug degradation and reaching the specific therapeutic target with lower doses.

Objective

evaluate the antifungal and immunomodulatory effect of ITZ encapsulated into poly(lactic-co-glycolic acid) (PLGA) NPs, administrated orally and intraperitoneally in an in vivo histoplasmosis model.

Results

After intranasal infection and treatment of animals with encapsulated ITZ by intraperitoneal and oral route, fungal burden control, biodistribution, immune response, and histopathology were evaluated. The results showed that the intraperitoneal administered and encapsulated ITZ has an effective antifungal effect, significantly reducing the Colony-Forming-Units (CFU) after the first doses and controlling the infection dissemination, with a higher concentration in the liver, spleen, and lung compared to the oral treatment. In addition, it produced a substantial immunomodulatory effect on pro- and anti-inflammatory cytokines and immune cell infiltrates confirmed by histopathology.

Conclusions

Overall, results suggest a synergistic effect of the encapsulated drug and the immunomodulatory effect contributing to infection control, preventing their dissemination.

导言荚膜组织胞浆菌是组织胞浆菌病的病原体,也是最常见的地方性肺霉菌病。伊曲康唑(ITZ)是轻症患者的首选药物,也是重症和播散性临床表现的降级疗法。将药物封装到纳米颗粒(NPs)中是提高药物溶解度和生物利用度、减少不良相互作用和药物降解以及以较低剂量达到特定治疗目标的一种替代方法。结果经鼻内感染后,用封装的 ITZ 通过腹腔和口服途径治疗动物,对真菌负荷控制、生物分布、免疫反应和组织病理学进行了评估。结果表明,腹腔给药和封装的ITZ具有有效的抗真菌作用,在首次给药后可显著减少菌落形成单位(CFU),并控制感染扩散,与口服治疗相比,ITZ在肝、脾和肺中的浓度更高。此外,经组织病理学证实,它还对促炎和抗炎细胞因子以及免疫细胞浸润产生了实质性的免疫调节作用。结论总体而言,研究结果表明封装药物的协同作用和免疫调节作用有助于控制感染,防止感染扩散。
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引用次数: 0
Risk of fungal exposure in the homes of patients with hematologic malignancies 血液系统恶性肿瘤患者家中接触真菌的风险
IF 3.6 4区 医学 Q3 MYCOLOGY Pub Date : 2024-06-08 DOI: 10.1016/j.mycmed.2024.101492
Marc Sautour , Adrien Guilloteau , Stéphane Valot , Louise Basmaciyan , Eloise Bailly , Nathalie Sixt , Jennifer Tetu , Ingrid Lafon , Denis Caillot , Frédéric Dalle

Background

Patients with hematological malignancies are at a high risk of developing invasive fungal infections (IFI) because they undergo several cycles of treatment leading to episodes of neutropenia. In addition, they alternate between hospital stays and periods spent at home. Thus, when an IFI is diagnosed during their hospital stays, it is highly challenging to identify the origin of the fungal contamination. The objective of this study was to analyze at home fungal exposure of 20 patients with leukemia by taking air and water samples in their living residence.

Methods

Air was sampled in 3 rooms of each home with a portable air system impactor. Tap water was collected at 3 water distribution points of each home. For positive samples, fungi were identified by mass spectrometry or on the basis of their morphological features.

Results

85 % of homes revealed the presence in air of Aspergillus spp. and those belonging to the section Fumigati presented the highest concentrations and the greatest frequency of isolation. Concerning mucorales, Rhizopus spp. and Mucor spp. were isolated in air of 20 % and 5 % of dwellings, respectively. In 4 homes, more than 70 % of the fungal species identified in air were potential opportunists; these were mainly Aspergillus spp. with concentrations greater than 20 cfu/m3. The water samples revealed the presence of Fusarium in 3 dwellings, with concentrations up to 80 cfu/L. Finally, for one patient, fungal species isolated during a period of hospitalization were phenotypically similar to those isolated in samples taken at home. For a second patient, a PCR Mucorale was positive on a sample of bronchoalveolar fluid while air samples taken at his home also revealed also the presence of mucorales.

Conclusion

The presence of opportunistic fungal species in the air of all the explored homes suggests the need for strengthened preventive measures in the home of immunocompromised patients. It would be interesting to compare the fungi isolated (from patients and from their environment) by genotyping studies aimed at specifying the correspondence existing between fungal species present in the patients’ homes and those responsible for IFI in the same patients.

背景血液系统恶性肿瘤患者罹患侵袭性真菌感染(IFI)的风险很高,因为他们要接受多个周期的治疗,导致中性粒细胞减少症的发作。此外,他们在住院期间和在家期间交替出现。因此,当他们在住院期间被诊断出 IFI 时,确定真菌污染的源头就非常具有挑战性。本研究的目的是通过采集 20 名白血病患者居住地的空气和水样本,分析他们在家中接触真菌的情况。在每个家庭的 3 个供水点采集自来水样本。结果85%的家庭发现空气中存在曲霉菌属,其中属于 Fumigati 类的曲霉菌属浓度最高,分离频率最高。在粘菌方面,20% 的住宅空气中分离出了根瘤菌属,5% 的住宅空气中分离出了粘孢子菌属。在 4 个住宅中,空气中 70% 以上的真菌种类是潜在的机会菌;主要是曲霉菌属,其浓度超过 20 cfu/m3。水样显示,3 个住宅中存在镰刀菌,浓度高达 80 cfu/L。最后,一名患者在住院期间分离出的真菌种类与在家中采集的样本中分离出的真菌种类在表型上相似。第二名患者的支气管肺泡液样本中的 PCR 粘孢子菌呈阳性,而在其家中采集的空气样本也显示存在粘孢子菌。有必要通过基因分型研究对(从患者及其环境中)分离出的真菌进行比较,以明确患者家中存在的真菌种类与导致同一患者 IFI 的真菌种类之间的对应关系。
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引用次数: 0
Novel griseofulvin zinc nanohybrid emulsion for intensifying the antimicrobial control of dermatophytes and some opportunistic pathogens 新型 Griseofulvin 锌纳米杂化乳液用于加强对皮癣菌和一些机会性病原体的抗菌控制
IF 2.2 4区 医学 Q3 MYCOLOGY Pub Date : 2024-06-04 DOI: 10.1016/j.mycmed.2024.101489
Farag M. Mosallam , Eman A. Helmy , Hebatallah A. Nasser , Ahmed I. El-Batal

Dermatophytosis is a critical sort of skin infection caused by dermatophytes. The long-term treatment of such skin infections may be improved through the application of nanotechnology. This study aimed to prepare griseofulvin zinc Nanohybrid emulsion (GF-Zn–NHE) to improve griseofulvin activity against dermatophytes and some opportunistic pathogenic yeasts and bacteria. The GF-Zn-NHE is prepared by ultra-homogenization ultra-sonication strategies and validated by UV–visible spectroscopy analysis that confirms presences of griseofulvin and Zn-NPs peaks at 265 and 360 nm, respectively. The GF-Zn-NHE has mean distribution size 50 nm and zeta potential in the range from -40 to -36 mV with no significant changes in size distribution and particle size within 120 day ageing. Fourier transform infrared spectroscopy spectrum confirmed the presence of griseofulvin and Zn-NPs stretching vibration peaks. Gamma ray has a negative influence on GF-Zn-NE production and stability. GF-Zn-NHE drug release 95% up to 24 h and 98% up to 72 h of GF was observed and Zinc 90% up to 24 h and 95% up to 72 h, respectively. High antimicrobial activity was observed with GF-Zn–NHE against dermatophytic pathogens in compare with GF, GF-NE, zinc nitrate and ketoconazole with inhibition zone ranged from 14 to 36 mm. The results have shown that the MIC value for Cryptococcus neoformans, Prophyromonas gingivalis and Pseudomonas aeruginosa is 0.125 mg ml −1 and for Trichophyton rubrum, L. bulgaricus and Escherichia coli value is 0.25 mg ml −1 and for Candida albicans, Malassezia furfur and Enterococcus faecalis is 0.5 mg ml −1 and finally 1 mg ml −1 for Streptococcus mutans. TEM of treated Cryptococcus neoformans cells with GF-Zn-NHE displayed essentially modified morphology, degradation, damage of organelles, vacuoles and other structures.

皮癣是由皮癣菌引起的一种严重的皮肤感染。应用纳米技术可改善此类皮肤感染的长期治疗。本研究旨在制备格列卫锌纳米杂化乳剂(GF-Zn-NHE),以提高格列卫对皮癣菌和一些机会致病性酵母菌和细菌的活性。GF-Zn-NHE 是通过超均质化超超声策略制备的,并通过紫外可见光谱分析进行了验证,证实在 265 纳米波长和 360 纳米波长处分别存在鬼臼毒素和 Zn-NPs 峰。GF-Zn-NHE 的平均分布尺寸为 50 纳米,zeta 电位在 -40 至 -36 mV 之间,在 120 天的老化过程中,尺寸分布和粒度没有发生显著变化。傅立叶变换红外光谱图证实了格列齐芬和 Zn-NPs 拉伸振动峰的存在。伽马射线对 GF-Zn-NE 的生成和稳定性有负面影响。观察到 GF-Zn-NHE 的药物释放率在 24 小时内为 95%,72 小时内为 98%,锌的药物释放率在 24 小时内为 90%,72 小时内为 95%。与 GF、GF-NE、硝酸锌和酮康唑相比,GF-锌-NHE 对皮真菌病原体具有较高的抗菌活性,抑菌区范围为 14 至 36 毫米。结果表明,对新生隐球菌、牙龈原虫和铜绿假单胞菌的 MIC 值为 0.125 毫克/毫升-1,对红色毛癣菌、保加利亚杆菌和大肠杆菌的 MIC 值为 0.25 毫克/毫升-1,对白色念珠菌、糠秕马拉色菌和粪肠球菌的 MIC 值为 0.5 毫克/毫升-1,最后对变异链球菌的 MIC 值为 1 毫克/毫升-1。用 GF-Zn-NHE 处理过的新生隐球菌细胞的 TEM 显示,其形态发生了本质上的改变,细胞器、空泡和其他结构发生了降解和破坏。
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引用次数: 0
Current knowledge and practice of Candida auris screening in France: A nationwide survey from the French Society of Medical Mycology (SFMM) 法国目前对念珠菌筛查的认识和实践:法国医学真菌学学会(SFMM)的一项全国性调查
IF 3.6 4区 医学 Q3 MYCOLOGY Pub Date : 2024-06-04 DOI: 10.1016/j.mycmed.2024.101490
J. Guitard , A.P. Bellanger , J. Dorin , S. Cassaing , A. Capitaine , F. Gabriel , M. Nicolas , N. Coron , P. Penn , M. Moniot , D. Quinio , S. Ranque , M. Sasso , P. Lepape , E. Dannaoui , S. Brun , C. Lacroix , M. Cornu , A. Debourgogne , M.F. Durieux , C. Hennequin

Due to large outbreaks observed worldwide, Candida auris has emerged as a major threat to healthcare facilities. To prevent these phenomena, a systematic screening should be performed in patients transferred from regions where the pathogen is highly endemic. In this study, we recorded and analyzed French mycologists' current knowledge and practice regarding C. auris screening and diagnosis. Thirty-six centers answered an online questionnaire. Only 11 (30.6 %) participants were aware of any systematic screening for C. auris for patients admitted to their hospital. In the case of post-admission screening, axillae/groins (n = 21), nares (n = 7), rectum (n = 9), and mouth (n = 6) alone or various combinations were the body sites the most frequently sampled. Only six centers (8.3 %) reported using a commercially available plate allowing the differentiation of C. auris colonies from that of other Candida species, while five laboratories (13.8 %) had implemented a C. auris-specific qPCR. Considering the potential impact on infected patients and the risk of disorganization in the care of patients, it is crucial to remember to biologists and clinicians the utmost importance of systematic screening on admission.

由于在全球范围内观察到的大规模疫情爆发,念珠菌已成为医疗机构的主要威胁。为了防止这些现象的发生,应该对从病原体高度流行地区转来的患者进行系统筛查。在这项研究中,我们记录并分析了法国真菌学专家目前对念珠菌筛查和诊断的认识和实践。36个中心回答了在线问卷。只有 11 位参与者(30.6%)知道他们医院对入院患者进行过系统的法氏囊病筛查。在入院后筛查中,腋窝/肛门(21 例)、鼻孔(7 例)、直肠(9 例)和口腔(6 例)是最常采样的身体部位。只有 6 个中心(8.3%)报告使用了市场上可买到的平板来区分白色念珠菌菌落和其他念珠菌菌落,而 5 个实验室(13.8%)使用了白色念珠菌特异性 qPCR。考虑到对受感染病人的潜在影响和病人护理混乱的风险,生物学家和临床医生必须牢记入院时进行系统筛查的极端重要性。
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引用次数: 0
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Journal de mycologie medicale
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