Dong-Jin Shin, C. Jo, Dongwook Kim, You-Ri Jung, Jun-Heon Lee, K. Nam, Hyo-Joon Choo, Aera Jang
{"title":"Taste-related and Volatile Organic Compounds of Fresh and Frozen–Thawed Chicken Breast Meat","authors":"Dong-Jin Shin, C. Jo, Dongwook Kim, You-Ri Jung, Jun-Heon Lee, K. Nam, Hyo-Joon Choo, Aera Jang","doi":"10.5187/jast.2023.e125","DOIUrl":"https://doi.org/10.5187/jast.2023.e125","url":null,"abstract":"","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"56 2","pages":""},"PeriodicalIF":2.3,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139280410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H. Cho, Minho Song, Ji Hwan Lee, H. Oh, Seju Chang, J. An, D. Song, Seung-Yeol Cho, Dong Jun Kim, Mi Suk Kim, Hyeun Bum Kim, Jin Ho Cho
Abstract
摘要
{"title":"Effects of phytogenic feed additives in growing and finishing pigs under different stocking density","authors":"H. Cho, Minho Song, Ji Hwan Lee, H. Oh, Seju Chang, J. An, D. Song, Seung-Yeol Cho, Dong Jun Kim, Mi Suk Kim, Hyeun Bum Kim, Jin Ho Cho","doi":"10.5187/jast.2023.e124","DOIUrl":"https://doi.org/10.5187/jast.2023.e124","url":null,"abstract":"Abstract","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"27 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139280461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Md. Hossain, S. Aung, Ji Park, Seon Kim, Sang Lee, Ki-El Nam
{"title":"Effects of Gender and Slaughter Age on Physicochemical and Functional Quality Traits of Korean Hanwoo Meat","authors":"Md. Hossain, S. Aung, Ji Park, Seon Kim, Sang Lee, Ki-El Nam","doi":"10.5187/jast.2023.e127","DOIUrl":"https://doi.org/10.5187/jast.2023.e127","url":null,"abstract":"","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"41 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139280471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Elick Kinara, J. Moturi, J. Mun, A. Hosseindoust, S. Ha, Se Rin Park, Pok Su Choi, SangIn Park, Jin Soo Kim
ABSTRACT
摘要
{"title":"Effects of Dietary Yeast β-Glucan on Lactating Sows under Heat Stress","authors":"Elick Kinara, J. Moturi, J. Mun, A. Hosseindoust, S. Ha, Se Rin Park, Pok Su Choi, SangIn Park, Jin Soo Kim","doi":"10.5187/jast.2023.e123","DOIUrl":"https://doi.org/10.5187/jast.2023.e123","url":null,"abstract":"ABSTRACT","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139281692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Seung Yun Lee, Da Young Lee, Ermie Jr. Mariano, Seung Hyeon Yun, Juhyun Lee, Jinmo Park, Yeongwoo Choi, Dahee Han, Jin Soo Kim, Seon-Tea Joo, Sun Jin Hur
This study aimed to analyze the leading research materials and research trends related to livestock food in Asia in recent years and propose future research agendas to ultimately contribute to the development of related livestock species. On analyzing more than 200 relevant articles, a high frequency of studies on livestock species and products with large breeding scales and vast markets was observed. Asia possesses the largest pig population and most extensive pork market, followed by that of beef, chicken, and milk; moreover, blood and egg markets have also been studied. Regarding research keywords, “meat quality” and “probiotics” were the most common, followed by “antioxidants,” which have been extensively studied in the past, and “cultured meat,” which has recently gained traction. The future research agenda for meat products is expected to be dominated by alternative livestock products, such as cultured and plant-derived meats; improved meat product functionality and safety; the environmental impacts of livestock farming; and animal welfare research. The future research agenda for dairy products is anticipated to include animal welfare, dairy production, probiotic-based development of high-quality functional dairy products, the development of alternative dairy products, and the advancement of lactose-free or personalized dairy products. However, determining the extent to which the various research articles’ findings have been applied in real-world industry proved challenging, and research related to animal food laws and policies and consumer surveys was lacking. In addition, studies on alternatives for sustainable livestock development could not be identified. Therefore, future research may augment industrial application, and multidisciplinary research related to animal food laws and policies as well as eco-friendly livestock production should be strengthened.
{"title":"Study on the current research trends and future agenda in animal products: an Asian perspective","authors":"Seung Yun Lee, Da Young Lee, Ermie Jr. Mariano, Seung Hyeon Yun, Juhyun Lee, Jinmo Park, Yeongwoo Choi, Dahee Han, Jin Soo Kim, Seon-Tea Joo, Sun Jin Hur","doi":"10.5187/jast.2023.e121","DOIUrl":"https://doi.org/10.5187/jast.2023.e121","url":null,"abstract":"This study aimed to analyze the leading research materials and research trends related to livestock food in Asia in recent years and propose future research agendas to ultimately contribute to the development of related livestock species. On analyzing more than 200 relevant articles, a high frequency of studies on livestock species and products with large breeding scales and vast markets was observed. Asia possesses the largest pig population and most extensive pork market, followed by that of beef, chicken, and milk; moreover, blood and egg markets have also been studied. Regarding research keywords, “meat quality” and “probiotics” were the most common, followed by “antioxidants,” which have been extensively studied in the past, and “cultured meat,” which has recently gained traction. The future research agenda for meat products is expected to be dominated by alternative livestock products, such as cultured and plant-derived meats; improved meat product functionality and safety; the environmental impacts of livestock farming; and animal welfare research. The future research agenda for dairy products is anticipated to include animal welfare, dairy production, probiotic-based development of high-quality functional dairy products, the development of alternative dairy products, and the advancement of lactose-free or personalized dairy products. However, determining the extent to which the various research articles’ findings have been applied in real-world industry proved challenging, and research related to animal food laws and policies and consumer surveys was lacking. In addition, studies on alternatives for sustainable livestock development could not be identified. Therefore, future research may augment industrial application, and multidisciplinary research related to animal food laws and policies as well as eco-friendly livestock production should be strengthened.","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"140 5","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135372234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bogun Kim, Ji yu Heo, Xiaoyue Xu, Hyunju Lee, Duleepa Pathiraja, Kim Jae-Young, Yi Hyun Choi, In-Geol Choi, Sae Hun Kim
It has been reported that the administration of Limosilactobacillus fermentum alleviates diseases such as osteoporosis and colitis. In this study, we report the complete genome sequence of Limosilactobacillusfermentum KUFM407, a probiotic strain of LAB isolated from Korean traditional fermented food, Kimchi. Whole genome sequencing of L. fermentum KUFM407 was performed on the Illumina MiSeq and Oxford Nanopore MinION platform. The genome consisted of one circular chromosome (2,077,616 bp) with a GC content of 51.5% and one circular plasmid sequence (13,931 bp). Genome annotation identified 1,932 protein-coding genes, 15 rRNAs, and 58 tRNAs in the assembly. The function annotation of the predicted proteins revealed genes involved in the biosynthesis of bacteriocin and fatty acids. The complete genome of L. fermentum KUFM407 could provide valuable information for the development of new probiotic food and health supplements.
{"title":"Complete genome sequence of candidate probiotic Limosilactobacillus fermentuum KUFM407","authors":"Bogun Kim, Ji yu Heo, Xiaoyue Xu, Hyunju Lee, Duleepa Pathiraja, Kim Jae-Young, Yi Hyun Choi, In-Geol Choi, Sae Hun Kim","doi":"10.5187/jast.2023.e122","DOIUrl":"https://doi.org/10.5187/jast.2023.e122","url":null,"abstract":"It has been reported that the administration of <italic>Limosilactobacillus fermentum</italic> alleviates diseases such as osteoporosis and colitis. In this study, we report the complete genome sequence of <italic>Limosilactobacillus</italic> <italic>fermentum</italic> KUFM407, a probiotic strain of LAB isolated from Korean traditional fermented food, Kimchi. Whole genome sequencing of <italic>L. fermentum</italic> KUFM407 was performed on the Illumina MiSeq and Oxford Nanopore MinION platform. The genome consisted of one circular chromosome (2,077,616 bp) with a GC content of 51.5% and one circular plasmid sequence (13,931 bp). Genome annotation identified 1,932 protein-coding genes, 15 rRNAs, and 58 tRNAs in the assembly. The function annotation of the predicted proteins revealed genes involved in the biosynthesis of bacteriocin and fatty acids. The complete genome of <italic>L. fermentum</italic> KUFM407 could provide valuable information for the development of new probiotic food and health supplements.","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"183 4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135371533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Korean native pigs (KNPs) have been one of the traditional livestock that have coexisted with the Korean people for centuries, primarily raised in rural areas of Korea. Korean native pigs have adapted to the climate and geography of Korean Peninsula for a long time, exhibiting excellent adaptability even in challenging environments. For these reasons, the preservation and purification of KNPs are crucial in securing unique genetic resources. Therefore, this review covers the characteristics, production status, commercial value and potential breeding directions of KNPs. Unfortunately, there is still a long way to go for the improvement of KNP. It is crucial to acknowledge the current challenges, identify the issues, and dedicate our efforts wholeheartedly to the breed
{"title":"The current status of Korean native pig production","authors":"Esther Lee, Jae-Cheol Jang, Sang-Hyon OH","doi":"10.5187/jast.2023.e120","DOIUrl":"https://doi.org/10.5187/jast.2023.e120","url":null,"abstract":"Korean native pigs (KNPs) have been one of the traditional livestock that have coexisted with the Korean people for centuries, primarily raised in rural areas of Korea. Korean native pigs have adapted to the climate and geography of Korean Peninsula for a long time, exhibiting excellent adaptability even in challenging environments. For these reasons, the preservation and purification of KNPs are crucial in securing unique genetic resources. Therefore, this review covers the characteristics, production status, commercial value and potential breeding directions of KNPs. Unfortunately, there is still a long way to go for the improvement of KNP. It is crucial to acknowledge the current challenges, identify the issues, and dedicate our efforts wholeheartedly to the breed","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"82 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136106529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sarbani Biswas, De Dang, Sungbo Cho, Dae-Kyung Kang, In Kim
This study was executed to estimate the impacts of increasing dietary standardized ileal digestible (SID) lysine and net energy levels on growth, nutrient absorption, and meat carcass traits in growing-finishing pigs. In total, 90 pigs [(Yorkshire × Landrace) × Duroc] were erratically dispensed to 3 treatments (6 replicate/treatment) with 5 pigs (3 barrows and 2 gilts) per pen, and their average primary body weight was 20.51± 0.02 kg. The trial period was 16 weeks (growing stage, initial to week 8; finishing stage, week 8 to week 16). The dietary treatments used included control (CON) as the basal diet, TRT1 (basal diet + 0.05% SID lysine), and TRT2 (basal diet + 0.05% SID lysine + 0.084 MJ/kg net energy) for both the growing and finishing stages. Both the TRT1 and TRT2 group diets improved (p = 0.033) average daily gain (ADG) at week 12 and tended to enhance (p = 0.088) body weight at week 12 and ADG at the overall period compared to the CON group. Moreover, pigs in the TRT2 group had higher backfat thickness (p = 0.034) at week 12 in comparison to the TRT1 and CON diets. Nevertheless, no treatment effect was found (p > 0.05) in nutrient absorption or carcass grade among the dietary treatments. Hence, incorporating the increasing level of 0.05% SID lysine and 0.084 MJ/kg net energy into the pig diet during the growing and finishing stages can be considered a suitable approach for enhancing both growth efficiency and carcass backfat thickness in pigs.
{"title":"Effects of increasing dietary lysine and energy levels on growth efficiency, nutrient absorption, and meat carcass traits in growing-finishing pigs","authors":"Sarbani Biswas, De Dang, Sungbo Cho, Dae-Kyung Kang, In Kim","doi":"10.5187/jast.2023.e118","DOIUrl":"https://doi.org/10.5187/jast.2023.e118","url":null,"abstract":"This study was executed to estimate the impacts of increasing dietary standardized ileal digestible (SID) lysine and net energy levels on growth, nutrient absorption, and meat carcass traits in growing-finishing pigs. In total, 90 pigs [(Yorkshire × Landrace) × Duroc] were erratically dispensed to 3 treatments (6 replicate/treatment) with 5 pigs (3 barrows and 2 gilts) per pen, and their average primary body weight was 20.51± 0.02 kg. The trial period was 16 weeks (growing stage, initial to week 8; finishing stage, week 8 to week 16). The dietary treatments used included control (CON) as the basal diet, TRT1 (basal diet + 0.05% SID lysine), and TRT2 (basal diet + 0.05% SID lysine + 0.084 MJ/kg net energy) for both the growing and finishing stages. Both the TRT1 and TRT2 group diets improved (<italic>p</italic> = 0.033) average daily gain (ADG) at week 12 and tended to enhance (<italic>p </italic>= 0.088) body weight at week 12 and ADG at the overall period compared to the CON group. Moreover, pigs in the TRT2 group had higher backfat thickness (<italic>p</italic> = 0.034) at week 12 in comparison to the TRT1 and CON diets. Nevertheless, no treatment effect was found (<italic>p</italic> &gt; 0.05) in nutrient absorption or carcass grade among the dietary treatments. Hence, incorporating the increasing level of 0.05% SID lysine and 0.084 MJ/kg net energy into the pig diet during the growing and finishing stages can be considered a suitable approach for enhancing both growth efficiency and carcass backfat thickness in pigs.","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"54 3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136106243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dongcheol Song, Jihwan Lee, yoonjeong Yoo, Hanjin Oh, Seyeon Chang, Jaewoo An, Sehyun Park, Kyeongho Jeon, Younghyun Cho, Yohan Yoon, Jinho Cho
This study aimed to evaluate the effects of mono- and multi-strain LAB probiotics on the growth performance, nutrient digestibility, blood profiles, fecal noxious gas emission, intestinal microbiota and intestinal morphology of weaning pigs challenged with or without Escherichia coli (E. coli) and Salmonella enterica (SE). In Exp. 1, a total of 60 crossbred weaning pigs were randomly allotted to one of five dietary treatments. The dietary treatments included: NC (negative control; basal diet with no supplement), PC (positive control; basal diet with 0.01% Lactiplantibacillus plantarum (LP) containing 1.0 × 108 CFU/g), K (basal diet with 0.1% Pediococcus acidilactici K (K) containing 1.0 × 109 CFU/g), WK1 (basal diet with 0.1% Pediococcus pentosaceus SMFM2016-WK1 (WK1) containing 1.0 × 109 CFU/g), K-WK1 (basal diet with 0.05% K + 0.05% WK1 containing 1.0 × 109 CFU/g). The average daily gain (ADG) was higher in the K group than in the WK1 group. Diarrhea score was lower in the K-WK1 group than in the NC group. At the genus level, Roseburia abundance in WK1 was higher than in the other treatment groups. At the species level, Blautia wexlerae abundance was lower in WK1 than in the other groups, whereas Succinivibrio dextrinosolvens abundance was higher in WK1. The serum pro-inflammatory cytokine levels in the PC and WK1 groups were as low as those in the NC group. Experiment 2 was conducted with two trials in a 2 × 5 factorial arrangement of treatments consisting of two levels of challenge (challenge and non-challenge) with E. coli and SE and five levels of probiotics same as Exp.1. Supplementation with LP and WK1 resulted in higher ADG and lower diarrhea scores than those in the other groups. Consequently, supplementation of WK1 showed a particularly positive effect on growth performance and diarrhea, villus height and intestinal microbiota in oral challenge experiment and feeding trial. Therefore, WK1 might be the most effective among the probiotics used in this experiment.
{"title":"Effects of probiotics on growth performance, intestinal morphology, intestinal microbiota weaning pig challenged with Escherichia coli and Salmonella enterica","authors":"Dongcheol Song, Jihwan Lee, yoonjeong Yoo, Hanjin Oh, Seyeon Chang, Jaewoo An, Sehyun Park, Kyeongho Jeon, Younghyun Cho, Yohan Yoon, Jinho Cho","doi":"10.5187/jast.2023.e119","DOIUrl":"https://doi.org/10.5187/jast.2023.e119","url":null,"abstract":"This study aimed to evaluate the effects of mono- and multi-strain LAB probiotics on the growth performance, nutrient digestibility, blood profiles, fecal noxious gas emission, intestinal microbiota and intestinal morphology of weaning pigs challenged with or without <italic>Escherichia coli (E. coli)</italic> and <italic>Salmonella enterica (</italic>SE<italic>)</italic>. In Exp. 1, a total of 60 crossbred weaning pigs were randomly allotted to one of five dietary treatments. The dietary treatments included: NC (negative control; basal diet with no supplement), PC (positive control; basal diet with 0.01% <italic>Lactiplantibacillus plantarum </italic>(LP) containing 1.0 × 10<sup>8</sup> CFU/g), K (basal diet with 0.1% <italic>Pediococcus acidilactici</italic> K (K) containing 1.0 × 10<sup>9</sup> CFU/g), WK1 (basal diet with 0.1% <italic>Pediococcus pentosaceus</italic> SMFM2016-WK1 (WK1) containing 1.0 × 10<sup>9</sup> CFU/g), K-WK1 (basal diet with 0.05% K + 0.05% WK1 containing 1.0 × 10<sup>9</sup> CFU/g). The average daily gain (ADG) was higher in the K group than in the WK1 group. Diarrhea score was lower in the K-WK1 group than in the NC group. At the genus level, <italic>Roseburia</italic> abundance in WK1 was higher than in the other treatment groups. At the species level, <italic>Blautia wexlerae</italic> abundance was lower in WK1 than in the other groups, whereas <italic>Succinivibrio dextrinosolvens</italic> abundance was higher in WK1. The serum pro-inflammatory cytokine levels in the PC and WK1 groups were as low as those in the NC group. Experiment 2 was conducted with two trials in a 2 × 5 factorial arrangement of treatments consisting of two levels of challenge (challenge and non-challenge) with <italic>E. coli</italic> and SE and five levels of probiotics same as Exp.1. Supplementation with LP and WK1 resulted in higher ADG and lower diarrhea scores than those in the other groups. Consequently, supplementation of WK1 showed a particularly positive effect on growth performance and diarrhea, villus height and intestinal microbiota in oral challenge experiment and feeding trial. Therefore, WK1 might be the most effective among the probiotics used in this experiment.","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"71 7","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136106530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Earwax, a semi-liquid substance, of both humans and non-human animals contains the stress hormone cortisol. Extraction of cortisol from earwax requires the use of organic solvents to eliminate interference factors. This study aimed to investigate the effects of organic solvents on the extraction efficiency of earwax cortisol from Hanwoo cattle. A factorial arrangement was used to determine the effects of solvent source (methyl alcohol, MA; diethyl ether, DE) and assay diluent buffer (ADB) (0 and 1 mL) on earwax cortisol concentration (ECC) in Hanwoo cattle (parity 3, body weight = 462 ± 5.6 kg). A 25 mg portion of the earwax sample was suspended in ADB, while another 25 mg portion was not suspended prior to extraction with MA and DE. ECC was measured using a commercial enzyme-linked immunosorbent assay cortisol kit. The results indicated that cortisol can be extracted from earwax with or without ADB. The extraction yield of cortisol from earwax was higher with MA than with DE (p < 0.05). In conclusion, MA is the preferred solvent for cortisol extraction owing to its high extraction yield, simple extraction process, and relatively low solvent consumption. Moreover, earwax can be used as a biomarker to evaluate the degree of chronic stress in Hanwoo cattle.
{"title":"Effect of organic solvents on earwax cortisol extraction in Hanwoo cattle","authors":"MOHAMMAD ATAALLAHI, Geun-Woo Park, Kyu-Hyun Park","doi":"10.5187/jast.2023.e117","DOIUrl":"https://doi.org/10.5187/jast.2023.e117","url":null,"abstract":"Earwax, a semi-liquid substance, of both humans and non-human animals contains the stress hormone cortisol. Extraction of cortisol from earwax requires the use of organic solvents to eliminate interference factors. This study aimed to investigate the effects of organic solvents on the extraction efficiency of earwax cortisol from Hanwoo cattle. A factorial arrangement was used to determine the effects of solvent source (methyl alcohol, MA; diethyl ether, DE) and assay diluent buffer (ADB) (0 and 1 mL) on earwax cortisol concentration (ECC) in Hanwoo cattle (parity 3, body weight = 462 ± 5.6 kg). A 25 mg portion of the earwax sample was suspended in ADB, while another 25 mg portion was not suspended prior to extraction with MA and DE. ECC was measured using a commercial enzyme-linked immunosorbent assay cortisol kit. The results indicated that cortisol can be extracted from earwax with or without ADB. The extraction yield of cortisol from earwax was higher with MA than with DE (<italic>p</italic> &lt; 0.05). In conclusion, MA is the preferred solvent for cortisol extraction owing to its high extraction yield, simple extraction process, and relatively low solvent consumption. Moreover, earwax can be used as a biomarker to evaluate the degree of chronic stress in Hanwoo cattle.","PeriodicalId":14923,"journal":{"name":"Journal of Animal Science and Technology","volume":"21 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134909492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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