Pub Date : 2023-10-16DOI: 10.1080/00218839.2023.2263718
Ali Hasan, Javed Iqbal Qazi, Fouzia Tabssum, Ali Hussain
AbstractA study about the provision of Lactobacillus rhamnosus NR_113332) and organic acids was carried out. 108 colony-forming units of probiotics were provided to the caged workers with and without the addition of 2.99% lactic acid, 2.91% acetic acid, and 1.96% acetic acid. After two weeks of oral administration, on termination, five bees were collected and dissected from each cage, and their midguts were cut into 8 μm thick sections by microtome. Subsequently, these histologically processed preparations were observed under camera fitted microscope. It was observed that the experimental workers were provided with diets: (pollen+ 50% w/v sucrose in 1.96% acetic acid, pollen+ L. rhamnosus in 50% w/v sucrose in distilled water, pollen+ L. rhamnosus in 50% w/v sucrose in 2.99% lactic acid, pollen+ L. rhamnosus in 50% w/v sucrose in 2.91% acetic acid, pollen+ L. rhamnosus in 50% w/v sucrose in 1.96% acetic acid) had a considerable quantity of peritrophic membranes. The mean midgut lumen diameters (µm2) were 373.33 ± 98.38, 296.67 ± 23.33, 243.33 ± 71.72, 426.67 ± 40.55, 576.67 ± 93.87 as compared to control’s 240.00 ± 30.55. The provision of probiotic and organic acids resulted in a slightly disturbed midgut epithelial wall, the presence of more quantity of peritrophic membranes, saved regeneration crypts, more gastric cells discharging, and some cytoplasmic vacuolization of caged worker bees. Our study suggests that probiotics and organic acids can be used as an environmentally friendly apipromotor, but verification by field studies is needed.Keywords: Probioticsorganic acidsmidgutApis melliferaHistological changes AcknowledgmentsThe role of Dr. Sohail Ahmed, lecturer, department of poultry science, University of Veterinary and animal sciences Lahore is much appreciable for assisting in statistical analyses.Disclosure statementThe authors declare that they have no competing interests.
{"title":"Midgut health of <i>Apis mellifera</i> workers on oral administration of <i>Lactobacillus rhamnosus</i> and organic acids","authors":"Ali Hasan, Javed Iqbal Qazi, Fouzia Tabssum, Ali Hussain","doi":"10.1080/00218839.2023.2263718","DOIUrl":"https://doi.org/10.1080/00218839.2023.2263718","url":null,"abstract":"AbstractA study about the provision of Lactobacillus rhamnosus NR_113332) and organic acids was carried out. 108 colony-forming units of probiotics were provided to the caged workers with and without the addition of 2.99% lactic acid, 2.91% acetic acid, and 1.96% acetic acid. After two weeks of oral administration, on termination, five bees were collected and dissected from each cage, and their midguts were cut into 8 μm thick sections by microtome. Subsequently, these histologically processed preparations were observed under camera fitted microscope. It was observed that the experimental workers were provided with diets: (pollen+ 50% w/v sucrose in 1.96% acetic acid, pollen+ L. rhamnosus in 50% w/v sucrose in distilled water, pollen+ L. rhamnosus in 50% w/v sucrose in 2.99% lactic acid, pollen+ L. rhamnosus in 50% w/v sucrose in 2.91% acetic acid, pollen+ L. rhamnosus in 50% w/v sucrose in 1.96% acetic acid) had a considerable quantity of peritrophic membranes. The mean midgut lumen diameters (µm2) were 373.33 ± 98.38, 296.67 ± 23.33, 243.33 ± 71.72, 426.67 ± 40.55, 576.67 ± 93.87 as compared to control’s 240.00 ± 30.55. The provision of probiotic and organic acids resulted in a slightly disturbed midgut epithelial wall, the presence of more quantity of peritrophic membranes, saved regeneration crypts, more gastric cells discharging, and some cytoplasmic vacuolization of caged worker bees. Our study suggests that probiotics and organic acids can be used as an environmentally friendly apipromotor, but verification by field studies is needed.Keywords: Probioticsorganic acidsmidgutApis melliferaHistological changes AcknowledgmentsThe role of Dr. Sohail Ahmed, lecturer, department of poultry science, University of Veterinary and animal sciences Lahore is much appreciable for assisting in statistical analyses.Disclosure statementThe authors declare that they have no competing interests.","PeriodicalId":15006,"journal":{"name":"Journal of Apicultural Research","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136113201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-05DOI: 10.1080/00218839.2023.2247115
Peter Neumann, Lars Straub
There is consensus that climate change is one of the grand challenges facing humanity in the twenty first century, inevitably the most confided and undeniably one of the most pressing. Profound effects are inevitable in global agriculture, and beekeeping is certainly no exemption. Indeed, extreme weather and natural disasters have already had an impact on honey bees. Thus, it appears evident that climate change will constitute a key stress factor for managed bees and beekeepers alike contributing to increased colony losses and reduced income. Here, we review the literature on the impact of climate change on honey bees and beekeeping. Based on the literature, it is evident that at present there is no inclusive strategy for beekeeping to adequately deal with the challenges global climate change will bring. Here, we call for such a strategy and briefly list the main challenges for future beekeeping due to a changing climate as well as suggest possible countermeasures. Ultimately, the impact of climate change and its mitigation are at present insufficiently understood in the beekeeping context. This calls for respective concerted efforts of scientists, beekeepers, and other stakeholders to find a sustainable future for beekeeping. Such efforts will inevitably require evidence-based mitigation measures to deal with the increasing global impact of climate change.
{"title":"Beekeeping under climate change","authors":"Peter Neumann, Lars Straub","doi":"10.1080/00218839.2023.2247115","DOIUrl":"https://doi.org/10.1080/00218839.2023.2247115","url":null,"abstract":"There is consensus that climate change is one of the grand challenges facing humanity in the twenty first century, inevitably the most confided and undeniably one of the most pressing. Profound effects are inevitable in global agriculture, and beekeeping is certainly no exemption. Indeed, extreme weather and natural disasters have already had an impact on honey bees. Thus, it appears evident that climate change will constitute a key stress factor for managed bees and beekeepers alike contributing to increased colony losses and reduced income. Here, we review the literature on the impact of climate change on honey bees and beekeeping. Based on the literature, it is evident that at present there is no inclusive strategy for beekeeping to adequately deal with the challenges global climate change will bring. Here, we call for such a strategy and briefly list the main challenges for future beekeeping due to a changing climate as well as suggest possible countermeasures. Ultimately, the impact of climate change and its mitigation are at present insufficiently understood in the beekeeping context. This calls for respective concerted efforts of scientists, beekeepers, and other stakeholders to find a sustainable future for beekeeping. Such efforts will inevitably require evidence-based mitigation measures to deal with the increasing global impact of climate change.","PeriodicalId":15006,"journal":{"name":"Journal of Apicultural Research","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135481786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-03DOI: 10.1080/00218839.2023.2262872
Molly McCann, Grace P. McCormack
Ireland is home to a significant population of the black bee, Apis mellifera mellifera, however, in recent years the numbers of imported queens have increased 10 fold and beekeepers are observing more bees that are not totally black. Here we confirm an increase in the proportion of introgressed honey bees in Ireland via multiple approaches. The use of colour and wing morphometrics is becoming less reliable in Ireland to identify native honey bees due to the increased presence of various hybrid phenotypes and genotypes.
{"title":"Increased levels of introgression evident in Irish honey bees","authors":"Molly McCann, Grace P. McCormack","doi":"10.1080/00218839.2023.2262872","DOIUrl":"https://doi.org/10.1080/00218839.2023.2262872","url":null,"abstract":"Ireland is home to a significant population of the black bee, Apis mellifera mellifera, however, in recent years the numbers of imported queens have increased 10 fold and beekeepers are observing more bees that are not totally black. Here we confirm an increase in the proportion of introgressed honey bees in Ireland via multiple approaches. The use of colour and wing morphometrics is becoming less reliable in Ireland to identify native honey bees due to the increased presence of various hybrid phenotypes and genotypes.","PeriodicalId":15006,"journal":{"name":"Journal of Apicultural Research","volume":"57 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135739415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-03DOI: 10.1080/00218839.2023.2262871
Carlos A. Yadró, Ana R. Lopes, Dora Henriques, Eduard Musin, Jakob Wegener, M. Alice Pinto
AbstractGenetic analysis of the honey bee spermathecal content can be particularly useful to provide an estimate of the genetic diversity and purity of the surrounding populations. Here we compared the concentration and quality of DNA extracted from queen spermatheca using four commercial kits to determine the best method to obtain DNA suitable for single nucleotide polymorphism genotyping by next-generation sequencing. The four kits were tested with different adjustments in the lysis incubation time, use of RNA-carrier, elution conditions and number of re-elutions. Only the use of QIAamp DNA Microkit with 3 h of lysis incubation, the addition of RNA-carrier and multiple re-elutions produced a DNA concentration over the required threshold.Keywords: Honey beespermathecagenomic DNA isolationDNA extraction Disclosure statementNo potential conflict of interest was reported by the authors.Additional informationFundingContributions of Jakob Wegener and Eduard Musin were supported by funds of the Federal Ministry of Food and Agriculture based on a decision of the Parliament of the Federal Republic of Germany via the Federal Office for Agriculture and Food, grant number 2818BM040. Dora Henriques was supported by BEEHAPPY (POCI-01-0145-FEDER-029871; FCT and COMPETE/QREN/EU). Ana R. Lopes and Carlos A. Yadró were supported by Fundação para a Ciência e a Tecnologia (FCT) grants SFRH/BD/143627/2019 and 2021.06948.BD, respectively. FCT provided financial support by national funds (FCT/MCTES) to CIMO (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2021).
摘要对蜜蜂精囊含量的遗传分析对于估计周围种群的遗传多样性和纯度是非常有用的。在此，我们比较了四种商用试剂盒中提取的精子后DNA的浓度和质量，以确定下一代测序获得适合单核苷酸多态性基因分型的DNA的最佳方法。对四种试剂盒在裂解孵育时间、rna载体的使用、洗脱条件和再洗脱次数等方面进行了不同的调整。仅使用QIAamp DNA Microkit，裂解孵育3小时，添加rna载体和多次再洗脱，即可产生超过所需阈值的DNA浓度。关键词:蜜蜂永久生物基因组DNA分离DNA提取披露声明作者未报告潜在利益冲突。资金:根据德意志联邦共和国议会通过联邦农业和粮食办公室的决定，Jakob Wegener和Eduard Musin的捐款由联邦粮食和农业部提供资金支持，批准号为2818BM040。Dora Henriques得到BEEHAPPY (poci -01-0145- federal -029871;FCT和COMPETE/QREN/EU)。Ana R. Lopes和Carlos a . Yadró得到了基金会(FCT)第Ciência段(SFRH/BD/143627/2019和2021.06948号基金)的资助。分别为双相障碍。FCT通过国家基金(FCT/MCTES)向CIMO (UIDB/00690/2020和UIDP/00690/2020)和SusTEC (LA/P/0007/2021)提供资金支持。
{"title":"Genomic DNA extraction from honey bee ( <i>Apis mellifera</i> ) queen spermathecal content","authors":"Carlos A. Yadró, Ana R. Lopes, Dora Henriques, Eduard Musin, Jakob Wegener, M. Alice Pinto","doi":"10.1080/00218839.2023.2262871","DOIUrl":"https://doi.org/10.1080/00218839.2023.2262871","url":null,"abstract":"AbstractGenetic analysis of the honey bee spermathecal content can be particularly useful to provide an estimate of the genetic diversity and purity of the surrounding populations. Here we compared the concentration and quality of DNA extracted from queen spermatheca using four commercial kits to determine the best method to obtain DNA suitable for single nucleotide polymorphism genotyping by next-generation sequencing. The four kits were tested with different adjustments in the lysis incubation time, use of RNA-carrier, elution conditions and number of re-elutions. Only the use of QIAamp DNA Microkit with 3 h of lysis incubation, the addition of RNA-carrier and multiple re-elutions produced a DNA concentration over the required threshold.Keywords: Honey beespermathecagenomic DNA isolationDNA extraction Disclosure statementNo potential conflict of interest was reported by the authors.Additional informationFundingContributions of Jakob Wegener and Eduard Musin were supported by funds of the Federal Ministry of Food and Agriculture based on a decision of the Parliament of the Federal Republic of Germany via the Federal Office for Agriculture and Food, grant number 2818BM040. Dora Henriques was supported by BEEHAPPY (POCI-01-0145-FEDER-029871; FCT and COMPETE/QREN/EU). Ana R. Lopes and Carlos A. Yadró were supported by Fundação para a Ciência e a Tecnologia (FCT) grants SFRH/BD/143627/2019 and 2021.06948.BD, respectively. FCT provided financial support by national funds (FCT/MCTES) to CIMO (UIDB/00690/2020 and UIDP/00690/2020) and SusTEC (LA/P/0007/2021).","PeriodicalId":15006,"journal":{"name":"Journal of Apicultural Research","volume":"94 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135697021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-18DOI: 10.1080/00218839.2023.2251201
Aleksandar Uzunov, Sreten Andonov, Bjørn Dahle, Marin Kovačić, Janez Prešern, Goran Aleksovski, Magdalena Jovanovska, Borce Pavlov, Zlatko Puškadija, Jakob Wegener, Egoitz Galartza, Ricarda Scheiner, Ralph Büchler
Honey bees (Apis mellifera) have a peculiar and complex reproductive biology, with queens being polyandrous and mating with several drones during one or more mating (nuptial) flights in so-called drone congregation areas. Observing the virgin queens’ and drones’ flight behaviour provides data to understand and interpret a portion of the honey bees’ complex reproductive process. Observing the behaviour of the virgin queens on the hive entrance also serves to estimate the distance from the mating place or potential drone congregation areas (DCAs) as well as to detect the presence of airborne drones in the area.
{"title":"Standard methods for direct observation of honey bee ( <i>Apis mellifera</i> L.) nuptial flights","authors":"Aleksandar Uzunov, Sreten Andonov, Bjørn Dahle, Marin Kovačić, Janez Prešern, Goran Aleksovski, Magdalena Jovanovska, Borce Pavlov, Zlatko Puškadija, Jakob Wegener, Egoitz Galartza, Ricarda Scheiner, Ralph Büchler","doi":"10.1080/00218839.2023.2251201","DOIUrl":"https://doi.org/10.1080/00218839.2023.2251201","url":null,"abstract":"Honey bees (Apis mellifera) have a peculiar and complex reproductive biology, with queens being polyandrous and mating with several drones during one or more mating (nuptial) flights in so-called drone congregation areas. Observing the virgin queens’ and drones’ flight behaviour provides data to understand and interpret a portion of the honey bees’ complex reproductive process. Observing the behaviour of the virgin queens on the hive entrance also serves to estimate the distance from the mating place or potential drone congregation areas (DCAs) as well as to detect the presence of airborne drones in the area.","PeriodicalId":15006,"journal":{"name":"Journal of Apicultural Research","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135148655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-05DOI: 10.1080/00218839.2023.2252126
Ç. Özenirler, A. Schiesser, Burcu Daşer-Özgişi
{"title":"Insights into the gut microbiome of local steppe-vegetation inhabitant bees: microbial community analysis of Bombus niveatus niveatus, Bombus niveatus vorticosus, Bombus terrestris, and Apis mellifera","authors":"Ç. Özenirler, A. Schiesser, Burcu Daşer-Özgişi","doi":"10.1080/00218839.2023.2252126","DOIUrl":"https://doi.org/10.1080/00218839.2023.2252126","url":null,"abstract":"","PeriodicalId":15006,"journal":{"name":"Journal of Apicultural Research","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2023-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47903139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.1080/00218839.2023.2250454
Y. Korichi, Khelfane-Goucem Karima, Aouar-Sadli Malika
{"title":"Diversity and foraging behavior assessment of watermelon pollinators in Tizi-Ouzou area (Algeria)","authors":"Y. Korichi, Khelfane-Goucem Karima, Aouar-Sadli Malika","doi":"10.1080/00218839.2023.2250454","DOIUrl":"https://doi.org/10.1080/00218839.2023.2250454","url":null,"abstract":"","PeriodicalId":15006,"journal":{"name":"Journal of Apicultural Research","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48039109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-01DOI: 10.1080/00218839.2023.2247840
Gizem Sonmez Oskay, Gulsen Sude Uygur, D. Oskay, Nazli Arda
{"title":"Impact of stress factors internal and external to the hive on honey bees and their reflection on honey bee products: a review","authors":"Gizem Sonmez Oskay, Gulsen Sude Uygur, D. Oskay, Nazli Arda","doi":"10.1080/00218839.2023.2247840","DOIUrl":"https://doi.org/10.1080/00218839.2023.2247840","url":null,"abstract":"","PeriodicalId":15006,"journal":{"name":"Journal of Apicultural Research","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44899980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pollinators or pillagers? What is the impact of flower visitors on the production of yellow passion fruit?","authors":"Zilda Cristina Malheiros Lima, Priscila Silva Miranda, R. Pérez-Maluf, Aldenise Alves Moreira","doi":"10.1080/00218839.2023.2252132","DOIUrl":"https://doi.org/10.1080/00218839.2023.2252132","url":null,"abstract":"","PeriodicalId":15006,"journal":{"name":"Journal of Apicultural Research","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46201580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-21DOI: 10.1080/00218839.2023.2245211
Şeyma Suyabatmaz, Şengül Alpay Karaoğlu, R. Akpinar, A. Bozdeveci, Selim Bıyık, A. Güler, Selma Kaya
{"title":"Inhibitive and prophylactic efficacy of lactic acid bacteria from Apis mellifera (Hymenoptera: Apidae) in combating Paenibacillus infections","authors":"Şeyma Suyabatmaz, Şengül Alpay Karaoğlu, R. Akpinar, A. Bozdeveci, Selim Bıyık, A. Güler, Selma Kaya","doi":"10.1080/00218839.2023.2245211","DOIUrl":"https://doi.org/10.1080/00218839.2023.2245211","url":null,"abstract":"","PeriodicalId":15006,"journal":{"name":"Journal of Apicultural Research","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2023-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44120475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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