Pub Date : 2015-05-25DOI: 10.4172/2167-0250.1000S1-001
H. Jeng, M. Chao, Ruei-Nian Li, C. Pan, Wen-Yi Lin
This study aimed to assess the correlation between 8-oxo-7,8-dihydro-2’-deoxyguanosine (8-oxo-dGuo) in semen and urine, and to compare the analytical methods of the isotope-diluted liquid chromatograph-tandem mass spectrometry (LC-MS/MS) coupled with an on-line Solid-Phase Extraction (SPE) and commercial Enzyme- Linked Immunosorbent Assay (ELISA) used for detecting 8-oxo-dGuo as an oxidative DNA damage marker. Semen and urine samples were simultaneously collected from 85 apparently healthy human subjects. An optimized DNA extraction method was employed to extract DNA from sperm while minimizing oxidation of DNA. All of the biological samples were analyzed by LC-MS/MS and ELISA. All of the biological samples were detected with 8-oxodGuo. ELISA consistently detected two to three times higher 8-oxodGuo levels in urine samples than LC-MS/MS. However, there was no significant correlation between measurements of 8-oxo-dGuo levels in urine and semen. In conclusion, the LC-MS/MS coupled with an SPE was a sensitive method to detect and quantify 8-oxo-dGuo in human sperm and urine. Urinary 8-oxo-dGuo may not be a reliable marker for detecting oxidatively damaged DNA in sperm.
{"title":"Measurement of 8-Oxo-7,8-Dihydro-2â-Deoxyguanosine in Human Semenand Urine by Isotope-Dilution Liquid Chromatography-Tandem MassSpectrometry with On-Line Solid-Phase Extraction: Comparison with aCommercial Available Enzyme-Linked Immunosorbent Assay","authors":"H. Jeng, M. Chao, Ruei-Nian Li, C. Pan, Wen-Yi Lin","doi":"10.4172/2167-0250.1000S1-001","DOIUrl":"https://doi.org/10.4172/2167-0250.1000S1-001","url":null,"abstract":"This study aimed to assess the correlation between 8-oxo-7,8-dihydro-2’-deoxyguanosine (8-oxo-dGuo) in semen and urine, and to compare the analytical methods of the isotope-diluted liquid chromatograph-tandem mass spectrometry (LC-MS/MS) coupled with an on-line Solid-Phase Extraction (SPE) and commercial Enzyme- Linked Immunosorbent Assay (ELISA) used for detecting 8-oxo-dGuo as an oxidative DNA damage marker. Semen and urine samples were simultaneously collected from 85 apparently healthy human subjects. An optimized DNA extraction method was employed to extract DNA from sperm while minimizing oxidation of DNA. All of the biological samples were analyzed by LC-MS/MS and ELISA. All of the biological samples were detected with 8-oxodGuo. ELISA consistently detected two to three times higher 8-oxodGuo levels in urine samples than LC-MS/MS. However, there was no significant correlation between measurements of 8-oxo-dGuo levels in urine and semen. In conclusion, the LC-MS/MS coupled with an SPE was a sensitive method to detect and quantify 8-oxo-dGuo in human sperm and urine. Urinary 8-oxo-dGuo may not be a reliable marker for detecting oxidatively damaged DNA in sperm.","PeriodicalId":15029,"journal":{"name":"Journal of andrology","volume":"2015 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2015-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4172/2167-0250.1000S1-001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70784992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-06-07DOI: 10.4172/2167-0250.1000117
Xiansheng Zhang, D. Tang, Jiajia Yang, K. Shi, Jingjing Gao, Z. Hao, Jun Zhou, C. Liang
Objective: To determine the efficacy and safety of sertraline monotherapy and combination therapy with sertraline and sildenafil in the treatment of APE without concomitant diseases. Methods: The study was conducted in 120 outpatients diagnosed with APE but without concomitant diseases. These patients were randomly divided into two groups: group A was treated with 50 mg sertraline daily; group B was treated with 50 mg sertraline daily and 50 mg sildenafil as needed. Assessment of the efficacy and safety of the two therapies was performed after 4 and 8 weeks. Patient or partner reports of Intravaginal Ejaculatory Latency Time (IELT), Premature Ejaculation Profile (PEP), Clinical Global Impression of Change (CGIC), and Treatment-Emergent Adverse Events (TEAEs) were assessed in this study. All the assessments were compared in the two groups after the treatment period. The efficacy was assessed by IELT, PEP and CGIC. On the other hand, safety was assessed by TEAEs. Results: 112 participants completed the study voluntarily. The two groups were similar regarding demographics. At the end of study period, both groups had significant improvements in IELT and PEP measures compared with pretreatment (P<0.001). Compared with group A, group B had significantly greater values of IELT (7.20 ± 2.93 vs. 5.04 ± 2.79), PEP measures, and CGIC (subjects reporting at least ‘better’: 58.2% vs. 35.8%) (P<0.05 for all). Adverse effects including headache, flushing, etc. were found in both groups, and the total incidence was higher in group B than group A (31.7% vs. 23.3%, respectively), but the difference was not significant. All the adverse effects were mild and tolerated. Conclusion: Both sertraline monotherapy and combination therapy with sildenafil and sertraline were efficacious and safe in the treatment of APE without concomitant diseases. The combination therapy had a higher efficacy than sertraline monotherapy without more adverse effects.
{"title":"Combination of Sertraline and Sildenafil versus Sertraline Monotherapy in the Treatment of Acquired Premature Ejaculation without Concomitant Diseases","authors":"Xiansheng Zhang, D. Tang, Jiajia Yang, K. Shi, Jingjing Gao, Z. Hao, Jun Zhou, C. Liang","doi":"10.4172/2167-0250.1000117","DOIUrl":"https://doi.org/10.4172/2167-0250.1000117","url":null,"abstract":"Objective: To determine the efficacy and safety of sertraline monotherapy and combination therapy with sertraline and sildenafil in the treatment of APE without concomitant diseases. \u0000Methods: The study was conducted in 120 outpatients diagnosed with APE but without concomitant diseases. These patients were randomly divided into two groups: group A was treated with 50 mg sertraline daily; group B was treated with 50 mg sertraline daily and 50 mg sildenafil as needed. Assessment of the efficacy and safety of the two therapies was performed after 4 and 8 weeks. Patient or partner reports of Intravaginal Ejaculatory Latency Time (IELT), Premature Ejaculation Profile (PEP), Clinical Global Impression of Change (CGIC), and Treatment-Emergent Adverse Events (TEAEs) were assessed in this study. All the assessments were compared in the two groups after the treatment period. The efficacy was assessed by IELT, PEP and CGIC. On the other hand, safety was assessed by TEAEs. \u0000Results: 112 participants completed the study voluntarily. The two groups were similar regarding demographics. At the end of study period, both groups had significant improvements in IELT and PEP measures compared with pretreatment (P<0.001). Compared with group A, group B had significantly greater values of IELT (7.20 ± 2.93 vs. 5.04 ± 2.79), PEP measures, and CGIC (subjects reporting at least ‘better’: 58.2% vs. 35.8%) (P<0.05 for all). Adverse effects including headache, flushing, etc. were found in both groups, and the total incidence was higher in group B than group A (31.7% vs. 23.3%, respectively), but the difference was not significant. All the adverse effects were mild and tolerated. \u0000Conclusion: Both sertraline monotherapy and combination therapy with sildenafil and sertraline were efficacious and safe in the treatment of APE without concomitant diseases. The combination therapy had a higher efficacy than sertraline monotherapy without more adverse effects.","PeriodicalId":15029,"journal":{"name":"Journal of andrology","volume":"3 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2014-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70784321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-05-27DOI: 10.4172/2167-0250.1000116
Swetasmita Mishra, Kranthi, R. Kumar, N. Malhotra, Kuldeep Mohanty, V. Pathak, R. Dada
Background: Sperm DNA is susceptible to oxidative damage due to intrinsic and extrinsic factors which cause oxidative stress and due to limited DNA damage detection and repair mechanism. Reactive oxygen species (ROS) are the chief cause of sperm DNA damage. So, this study was planned to assess oxidative stress levels and correlate with sperm DNA damage. Material and Method: The study included 35 men who had fathered a child in the last one year and 54 male partners of couple experiencing primary infertility. Semen analysis was done according to World Health Organization (1999) criteria. ROS measurement was done by direct chemiluminescence method using luminol as a probe. DNA damage was assessed by sperm chromatin structure assay (SCSA) and expressed as percentage DFI. 8- Hydroxy-2’-deoxyguanosine (8-OHdG) estimation was carried out by competitive ELISA. Results: The seminal ROS level (RLU/sec/million sperm) was significantly higher (40.52 ± 18.32) in infertile men as compared to fertile controls (14.04 ± 6.67) (p<0.0001). The mean values of 8-OHdG levels (pg/ml) were also significantly higher in patients (30.92 ± 3.27) as compared to fertile controls (14.29 ± 2.24) (p<0.0001) and mean DFI (%) of infertile men was found to be 35.48 ± 12.95, which was higher as compared to controls (24.18 ± 8.76). There was a strong positive correlation between these parameters. Conclusion: Majority of the sperm DNA damage in infertile men is caused by oxidative damage to the genomic DNA. In presence of limited DNA damage detection and repair mechanism in sperm, prevention of oxidative stress by simple lifestyle interventions may actually be therapeutic.
{"title":"Oxidative Damage to Sperm DNA: Clinical Implications","authors":"Swetasmita Mishra, Kranthi, R. Kumar, N. Malhotra, Kuldeep Mohanty, V. Pathak, R. Dada","doi":"10.4172/2167-0250.1000116","DOIUrl":"https://doi.org/10.4172/2167-0250.1000116","url":null,"abstract":"Background: Sperm DNA is susceptible to oxidative damage due to intrinsic and extrinsic factors which cause oxidative stress and due to limited DNA damage detection and repair mechanism. Reactive oxygen species (ROS) are the chief cause of sperm DNA damage. So, this study was planned to assess oxidative stress levels and correlate with sperm DNA damage. \u0000Material and Method: The study included 35 men who had fathered a child in the last one year and 54 male partners of couple experiencing primary infertility. Semen analysis was done according to World Health Organization (1999) criteria. ROS measurement was done by direct chemiluminescence method using luminol as a probe. DNA damage was assessed by sperm chromatin structure assay (SCSA) and expressed as percentage DFI. 8- Hydroxy-2’-deoxyguanosine (8-OHdG) estimation was carried out by competitive ELISA. \u0000Results: The seminal ROS level (RLU/sec/million sperm) was significantly higher (40.52 ± 18.32) in infertile men as compared to fertile controls (14.04 ± 6.67) (p<0.0001). The mean values of 8-OHdG levels (pg/ml) were also significantly higher in patients (30.92 ± 3.27) as compared to fertile controls (14.29 ± 2.24) (p<0.0001) and mean DFI (%) of infertile men was found to be 35.48 ± 12.95, which was higher as compared to controls (24.18 ± 8.76). There was a strong positive correlation between these parameters. \u0000Conclusion: Majority of the sperm DNA damage in infertile men is caused by oxidative damage to the genomic DNA. In presence of limited DNA damage detection and repair mechanism in sperm, prevention of oxidative stress by simple lifestyle interventions may actually be therapeutic.","PeriodicalId":15029,"journal":{"name":"Journal of andrology","volume":"3 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2014-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70784027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-05-25DOI: 10.4172/2167-0250.1000118
F. Dursun, E. Malkoç, S. Okçelik, A. Çırakoğlu, F. Ates, K. Karademir, T. Şenkul, H. Soydan
Aim: To determine whether there is a change in sexual satisfaction and self-esteem in adult patients with monosymptomatic enuresis. Methods: This study was a prospective questionnaire analysis. 35 adult age individuals still suffering from monosymptomatic enuresis filled out 5-item Arizona Sexual Experiences (ASEX) scale and the 10-item Self-Esteem Scale (SES). Same query forms were filled out by 23 similar aged healthy control group and the results were compared. A value of P<0.05 was used as significance limit. Results: Mean age of the 35 individuals included in the study was 21.6 ± 1.39 for the patient group and 22 ± 2.59 for the control group (n:23). While 7 patients in the enuresis group had sexual dysfunction, there was only in 1 patient in the control group. The self-esteem score has decreased in 22 respondents in patient group whereas there wasn't any decline in the self-esteem of control group. The two groups' responses to SES questions were statistically different (p<0.02). Conclusion: Monosymptomatic enuresis at adult ages might affect body respect negatively as well as it might have negative effects on sexual function independent of lower urinary tract symptoms.
{"title":"The Effect of Nocturnal Enuresis in Adults on Sexual Satisfaction and Self-Esteem","authors":"F. Dursun, E. Malkoç, S. Okçelik, A. Çırakoğlu, F. Ates, K. Karademir, T. Şenkul, H. Soydan","doi":"10.4172/2167-0250.1000118","DOIUrl":"https://doi.org/10.4172/2167-0250.1000118","url":null,"abstract":"Aim: To determine whether there is a change in sexual satisfaction and self-esteem in adult patients with monosymptomatic enuresis. \u0000Methods: This study was a prospective questionnaire analysis. 35 adult age individuals still suffering from monosymptomatic enuresis filled out 5-item Arizona Sexual Experiences (ASEX) scale and the 10-item Self-Esteem Scale (SES). Same query forms were filled out by 23 similar aged healthy control group and the results were compared. A value of P<0.05 was used as significance limit. \u0000Results: Mean age of the 35 individuals included in the study was 21.6 ± 1.39 for the patient group and 22 ± 2.59 for the control group (n:23). While 7 patients in the enuresis group had sexual dysfunction, there was only in 1 patient in the control group. The self-esteem score has decreased in 22 respondents in patient group whereas there wasn't any decline in the self-esteem of control group. The two groups' responses to SES questions were statistically different (p<0.02). \u0000Conclusion: Monosymptomatic enuresis at adult ages might affect body respect negatively as well as it might have negative effects on sexual function independent of lower urinary tract symptoms.","PeriodicalId":15029,"journal":{"name":"Journal of andrology","volume":"3 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2014-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70783902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-03-31DOI: 10.4172/2167-0250.1000115
G. Parenti, U. Giorgi, Elisa Gaddoni, V. Conteduca, S. Zago, P. Campioni, M. Giganti, F. Albarello
Purpose: To evaluate the association between grade II and III testicular Microlithiasis (TM) and Testicular Germ Cell Tumor (TGCT), reporting disease-free survival over 7 years. Materials and Methods: The association between TM and TGCT was studied in 7,320 male patients referred to the radiology department of an Italian hospital for several scrotal disease. TM associated with TCGT was diagnosed in all men by ultrasound (US) and by testicular histology specimens. All patients with TM were followed-up annually with US. Chi-square, Kaplan-Meyer and Fisher’s exact test were used for statistical analysis. Results: The incidence of TM was 1.4% (98 of 7320). Twenty eight patients with TGCT (28 of 58, 48.2%) had associated TM. During the follow-up, testicular cancer was detected in six patients (incidence 6.12%, 95% confidence interval from 2.8 to 12.7); four of these were recurrences of TGCT, while the other two patients were ex novo TGCT. There was a significant difference (p < 0.001) between the rate of TGCT in men with TM (28 of 98, 28.5%) and that in men without TM (30 of 7222, 0.4%) with an odds ratio of 95.89 [95% CI 42.7 - 110.5]. Conclusion: The association found between TGCT and TM addresses microlithiasis as a prospective marker for testicular tumor. Yearly follow-up with US should be taken into consideration, encouraging self-examination in the meantime.
{"title":"Testicular Microlithiasis and Testicular Germ Cell Tumors: A Seven Year Retrospective Study","authors":"G. Parenti, U. Giorgi, Elisa Gaddoni, V. Conteduca, S. Zago, P. Campioni, M. Giganti, F. Albarello","doi":"10.4172/2167-0250.1000115","DOIUrl":"https://doi.org/10.4172/2167-0250.1000115","url":null,"abstract":"Purpose: To evaluate the association between grade II and III testicular Microlithiasis (TM) and Testicular Germ Cell Tumor (TGCT), reporting disease-free survival over 7 years. \u0000Materials and Methods: The association between TM and TGCT was studied in 7,320 male patients referred to the radiology department of an Italian hospital for several scrotal disease. TM associated with TCGT was diagnosed in all men by ultrasound (US) and by testicular histology specimens. All patients with TM were followed-up annually with US. Chi-square, Kaplan-Meyer and Fisher’s exact test were used for statistical analysis. \u0000Results: The incidence of TM was 1.4% (98 of 7320). Twenty eight patients with TGCT (28 of 58, 48.2%) had associated TM. During the follow-up, testicular cancer was detected in six patients (incidence 6.12%, 95% confidence interval from 2.8 to 12.7); four of these were recurrences of TGCT, while the other two patients were ex novo TGCT. There was a significant difference (p < 0.001) between the rate of TGCT in men with TM (28 of 98, 28.5%) and that in men without TM (30 of 7222, 0.4%) with an odds ratio of 95.89 [95% CI 42.7 - 110.5]. \u0000Conclusion: The association found between TGCT and TM addresses microlithiasis as a prospective marker for testicular tumor. Yearly follow-up with US should be taken into consideration, encouraging self-examination in the meantime.","PeriodicalId":15029,"journal":{"name":"Journal of andrology","volume":"3 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2014-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70783972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-02-25DOI: 10.4172/2167-0250.1000114
T. Nadapdap, D. Lutan, Khm Arsyad, S. Ilyas
Background: Pollution Plumbum (Pb) has become a public health problem in the world, especially in developing countries, such as Asia, Africa and Latin America. For the humans to cause disturbances in the body’s organs such as the testes, will indirectly affect the quantity and quality of sperm. Aim: In the present study, the ability of lead to adversely affect the male reproductive system was investigated and chitosan (CHn) was administered orally to prevent the adverse effects of Pb. Materials and methods: Thirty Wistar rats, randomised into six groups (n=5), were used for this study. Distilled water administration for 7 weeks (negative control), Pb for 2 weeks + Pb and distilled water for 5 weeks (positive control), Pb for 2 weeks + Pb and chitosan -0.5% for 5 weeks (treatment-1), Pb for 2 weeks + Pb and chitosan-0,75% for 5 weeks (treatment-2), Pb for 2 weeks + Pb and chitosan-1% for 5 weeks (treatment-3). All treatments were for 7 weeks. Statistical Analysis: Kruskal-Wallis and Mann–Whitney U-test were used to analyse the results obtained. Results: The obtained results showed that Pb caused a significant reduction in the sperm count, sperm motility, normal sperm morphology, and sperm viability, but not a significant increase in ratio of testis/100 g body weight of rat. Chitosan, however, significantly reduced these adverse effects of Pb in cauda epididimis (sperm count, sperm motility, normal sperm morphology, and sperm viability). Conclusion: These findings lead to the conclusion that chitosan significantly lowered the adverse effects of Pb exposure on the testis as well as Pb-induced oxidative stress.
{"title":"Influence of Chitosan from Shrimp Skin to Quality and Quantity of Sperm of Albino Rats after Administration of Lead","authors":"T. Nadapdap, D. Lutan, Khm Arsyad, S. Ilyas","doi":"10.4172/2167-0250.1000114","DOIUrl":"https://doi.org/10.4172/2167-0250.1000114","url":null,"abstract":"Background: Pollution Plumbum (Pb) has become a public health problem in the world, especially in developing countries, such as Asia, Africa and Latin America. For the humans to cause disturbances in the body’s organs such as the testes, will indirectly affect the quantity and quality of sperm. \u0000Aim: In the present study, the ability of lead to adversely affect the male reproductive system was investigated and chitosan (CHn) was administered orally to prevent the adverse effects of Pb. \u0000Materials and methods: Thirty Wistar rats, randomised into six groups (n=5), were used for this study. Distilled water administration for 7 weeks (negative control), Pb for 2 weeks + Pb and distilled water for 5 weeks (positive control), Pb for 2 weeks + Pb and chitosan -0.5% for 5 weeks (treatment-1), Pb for 2 weeks + Pb and chitosan-0,75% for 5 weeks (treatment-2), Pb for 2 weeks + Pb and chitosan-1% for 5 weeks (treatment-3). All treatments were for 7 weeks. \u0000Statistical Analysis: Kruskal-Wallis and Mann–Whitney U-test were used to analyse the results obtained. \u0000Results: The obtained results showed that Pb caused a significant reduction in the sperm count, sperm motility, normal sperm morphology, and sperm viability, but not a significant increase in ratio of testis/100 g body weight of rat. Chitosan, however, significantly reduced these adverse effects of Pb in cauda epididimis (sperm count, sperm motility, normal sperm morphology, and sperm viability). \u0000Conclusion: These findings lead to the conclusion that chitosan significantly lowered the adverse effects of Pb exposure on the testis as well as Pb-induced oxidative stress.","PeriodicalId":15029,"journal":{"name":"Journal of andrology","volume":"3 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2014-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70783922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2014-02-25DOI: 10.4172/2167-0250.1000113
Ashraf Cm, P. Dharmaraj, S. Sankalp, R. Sujatha, S. Swati, D. Vijayalakshmi, Esteves Sc
Objective: We describe our micro-TESE experience in a large group of men with Non-Obstructive Azoospermia (NOA) and poor prognosis for Sperm Retrieval (SR), and critically analyze the method´s results and limitations. Methods: An ART facility was setup in a tertiary care center to perform SR using microsurgery. One hundred and eighty men with NOA underwent micro-TESE while their female partners received ovarian stimulation for Oocyte Pickup (OCP). Micro-TESE was performed on the day prior to OCP, and surgically-retrieved testicular sperm were used for sperm injections. We assessed sperm retrieval rates, operative aspects, and ICSI outcomes. Results: The success of micro-TESE at obtaining testicular sperm for Intracytoplasmic Sperm Injection (ICSI) was 54.4% with no major complications. Sperm were obtained in 73.6 % of cases in which clearly dilated seminiferous tubules were seen, with minimal tissue excision which facilitated laboratory processing. Patients with successful and failed retrievals did not differ with respect to baseline characteristics, and presence of varicocele. Retrieval rates differed pertaining to testicular histology category. Also, retrieval rates were higher (53.1% vs. 35.6%) in patients who received medication to boost testosterone production prior to micro-TESE compared with those who did not. Sperm injections resulted in normal fertilization and embryo cleavage of 61% and 75%, respectively. A cumulative clinical pregnancy rate per ICSI cycle of 29.78 %, with an implantation rate of 19 %was achieved. Conclusions: Micro-TESE is a valid method of SR in NOA. It yields sustainable results in poor prognosis azoospermic patients, with minimal damage to the testes. Our experience with micro-TESE applied to the most difficult cases of azoospermia is very reassuring, and we advocate that micro-TESE should be the method of choice in such cases.
目的:我们描述了我们在一大群非阻塞性无精子症(NOA)和预后不良的男性精子回收(SR)中的微tese经验，并批判性地分析了该方法的结果和局限性。方法:在某三级保健中心设置ART设施，采用显微外科技术进行SR手术。180名患有NOA的男性接受了显微tese，而他们的女性伴侣则接受了卵巢刺激以获取卵母细胞(OCP)。在OCP前一天进行Micro-TESE，手术取出的睾丸精子用于精子注射。我们评估了精子回收率、手术方面和ICSI结果。结果:显微tese取精成功率为54.4%，无重大并发症。精子在73.6%的病例中得到明显扩张的精管，最小的组织切除，方便实验室处理。成功和失败的患者在基线特征和精索静脉曲张的存在方面没有差异。睾丸组织类型不同，恢复率也不同。此外，与未接受micro-TESE治疗的患者相比，在micro-TESE治疗前接受促进睾酮产生药物治疗的患者恢复率更高(53.1% vs. 35.6%)。注射精子的受精率为61%，胚胎卵裂率为75%。每个ICSI周期的累积临床妊娠率为29.78%，着床率为19%。结论:显微tese是一种有效的NOA SR测定方法。它对预后不良的无精子患者产生持续的结果，对睾丸的损害最小。我们将微型tese应用于最困难的无精子症病例的经验非常令人放心，我们主张在这种情况下应该选择微型tese方法。
{"title":"Microdissection Testicular Sperm Extraction (Micro-TESE): Results of a Large Series from India","authors":"Ashraf Cm, P. Dharmaraj, S. Sankalp, R. Sujatha, S. Swati, D. Vijayalakshmi, Esteves Sc","doi":"10.4172/2167-0250.1000113","DOIUrl":"https://doi.org/10.4172/2167-0250.1000113","url":null,"abstract":"Objective: We describe our micro-TESE experience in a large group of men with Non-Obstructive Azoospermia (NOA) and poor prognosis for Sperm Retrieval (SR), and critically analyze the method´s results and limitations. \u0000Methods: An ART facility was setup in a tertiary care center to perform SR using microsurgery. One hundred and eighty men with NOA underwent micro-TESE while their female partners received ovarian stimulation for Oocyte Pickup (OCP). Micro-TESE was performed on the day prior to OCP, and surgically-retrieved testicular sperm were used for sperm injections. We assessed sperm retrieval rates, operative aspects, and ICSI outcomes. \u0000Results: The success of micro-TESE at obtaining testicular sperm for Intracytoplasmic Sperm Injection (ICSI) was 54.4% with no major complications. Sperm were obtained in 73.6 % of cases in which clearly dilated seminiferous tubules were seen, with minimal tissue excision which facilitated laboratory processing. Patients with successful and failed retrievals did not differ with respect to baseline characteristics, and presence of varicocele. Retrieval rates differed pertaining to testicular histology category. Also, retrieval rates were higher (53.1% vs. 35.6%) in patients who received medication to boost testosterone production prior to micro-TESE compared with those who did not. Sperm injections resulted in normal fertilization and embryo cleavage of 61% and 75%, respectively. A cumulative clinical pregnancy rate per ICSI cycle of 29.78 %, with an implantation rate of 19 %was achieved. \u0000Conclusions: Micro-TESE is a valid method of SR in NOA. It yields sustainable results in poor prognosis azoospermic patients, with minimal damage to the testes. Our experience with micro-TESE applied to the most difficult cases of azoospermia is very reassuring, and we advocate that micro-TESE should be the method of choice in such cases.","PeriodicalId":15029,"journal":{"name":"Journal of andrology","volume":"3 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2014-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70783886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-12-05DOI: 10.4172/2167-0250.1000111
C. Palma, J. Vinay, J. Sàrquella, J. Sanchez, Ariel Castro, Cesar Rojas-Cruz, F. Algaba, I. Gallegos, R. Rodrigo
The aim of this study is to determine the presence of adipocyte accumulation under penile tunica albuginea in a group of refractory erectile dysfunction patients. Nine erectile dysfunction patients (case group) and eleven potent patients with Peyronie’s disease (control group) underwent penile prosthesis implantation and curvature correction surgeries, respectively. In both groups, sub-albuginean tissue samples were taken within the operative time. Groups were compared in terms of clinical characteristics, co-morbidities and presence of sub-albuginean adipocyte accumulation. Of the nine patients in the case group, eight presented cavernous fat cell accumulation, while only one patient in the control group presented this finding (p<0.05). A significant association (p<0.05) was found between adipocyte accumulation and erectile dysfunction (OR 35 CI 95% 1.98-1727.62). A similar association with chronic arterial hypertension was also found (OR 20 CI 95% 1.29-1008.46). This is the first human study to report an association between erectile dysfunction and penile sub-albuginean fat accumulation. Metabolic syndrome-related conditions could cause disruption in androgen homeostasis, leading to adipocyte accumulation. Venous leakage secondary to accumulation of fat under tunica albuginea could be an important element in the pathophysiology of erectile dysfunction, especially in metabolic syndrome patients that do not respond to medical therapy.
本研究的目的是确定一组难治性勃起功能障碍患者阴茎白膜下脂肪细胞堆积的存在。9例勃起功能障碍患者(病例组)和11例强直性Peyronie病患者(对照组)分别行阴茎假体植入术和阴茎弯曲矫正术。两组均在手术时间内采集亚白蛋白组织样本。比较各组的临床特征、合并症和亚白蛋白脂肪细胞堆积的存在。病例组9例患者中，8例出现海绵状脂肪细胞堆积，对照组仅有1例出现海绵状脂肪细胞堆积(p<0.05)。脂肪细胞堆积与勃起功能障碍之间存在显著相关性(p<0.05) (OR 35 CI 95% 1.98-1727.62)。与慢性动脉高血压也发现了类似的关联(OR 20 CI 95% 1.29-1008.46)。这是第一个报道勃起功能障碍和阴茎亚白蛋白脂肪堆积之间关系的人类研究。代谢综合征相关的疾病可能导致雄激素稳态的破坏，导致脂肪细胞的积累。白膜下脂肪堆积引起的静脉渗漏可能是勃起功能障碍病理生理学的一个重要因素，尤其是在对药物治疗无效的代谢综合征患者中。
{"title":"Sub-Albuginean Adipocyte Accumulation is Associated with Erectile Dysfunction: First Clinical Evidence and Pathophysiological Implications","authors":"C. Palma, J. Vinay, J. Sàrquella, J. Sanchez, Ariel Castro, Cesar Rojas-Cruz, F. Algaba, I. Gallegos, R. Rodrigo","doi":"10.4172/2167-0250.1000111","DOIUrl":"https://doi.org/10.4172/2167-0250.1000111","url":null,"abstract":"The aim of this study is to determine the presence of adipocyte accumulation under penile tunica albuginea in a group of refractory erectile dysfunction patients. Nine erectile dysfunction patients (case group) and eleven potent patients with Peyronie’s disease (control group) underwent penile prosthesis implantation and curvature correction surgeries, respectively. In both groups, sub-albuginean tissue samples were taken within the operative time. Groups were compared in terms of clinical characteristics, co-morbidities and presence of sub-albuginean adipocyte accumulation. Of the nine patients in the case group, eight presented cavernous fat cell accumulation, while only one patient in the control group presented this finding (p<0.05). A significant association (p<0.05) was found between adipocyte accumulation and erectile dysfunction (OR 35 CI 95% 1.98-1727.62). A similar association with chronic arterial hypertension was also found (OR 20 CI 95% 1.29-1008.46). This is the first human study to report an association between erectile dysfunction and penile sub-albuginean fat accumulation. Metabolic syndrome-related conditions could cause disruption in androgen homeostasis, leading to adipocyte accumulation. Venous leakage secondary to accumulation of fat under tunica albuginea could be an important element in the pathophysiology of erectile dysfunction, especially in metabolic syndrome patients that do not respond to medical therapy.","PeriodicalId":15029,"journal":{"name":"Journal of andrology","volume":"2 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2013-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70784248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-11-27DOI: 10.4172/2167-0250.1000110
Tian Yang, Jie Zhao, Wei Li
Selective regulation of gene transcription is clearly important for cells to orchestrate an adaptive response to heat stress. We previously showed that Metastasis Associated Protein 1 (MTA1), a component of the nucleosome remodeling and deacetylase (NuRD) complex, operates as a negative coregulator of p53 in the maintenance of the apoptotic balance in pachytene spermatocytes (PS) after heat stress, although specific mechanisms are not well defined. The purpose of the current study was to investigate potential upstream signaling events activating MTA1 pathway in murine PS. Using murine sialoadenectomy model, it was demonstrated that deprivation of circulated EGF significantly impaired the in vivo expression of MTA1 in the PS. The upstream regulation of MTA1 expression during meiosis by endogenous EGF was further confirmed in vitro using a selective Epidermal Growth Factor Receptor (EGFR) inhibitor, tryphostin AG1478. Moreover, inhibition of EGF signaling by AG1478 treatment significantly suppressed the heat stress-induced MTA1 in PS. The available data collectively suggest that EGF signaling regulates the expression of MTA1 in PS, and early activation of EGF/MTA1 cascade in PS in response to heat stress may serve as an intrinsic self-defensive mechanism maintaining apoptotic balance during meiotic heat stress.
{"title":"Epidermal Growth Factor Signaling Regulates the Expression of Metastasis Tumor Antigen 1 in Mouse Pachytene Spermatocyte","authors":"Tian Yang, Jie Zhao, Wei Li","doi":"10.4172/2167-0250.1000110","DOIUrl":"https://doi.org/10.4172/2167-0250.1000110","url":null,"abstract":"Selective regulation of gene transcription is clearly important for cells to orchestrate an adaptive response to heat stress. We previously showed that Metastasis Associated Protein 1 (MTA1), a component of the nucleosome remodeling and deacetylase (NuRD) complex, operates as a negative coregulator of p53 in the maintenance of the apoptotic balance in pachytene spermatocytes (PS) after heat stress, although specific mechanisms are not well defined. The purpose of the current study was to investigate potential upstream signaling events activating MTA1 pathway in murine PS. Using murine sialoadenectomy model, it was demonstrated that deprivation of circulated EGF significantly impaired the in vivo expression of MTA1 in the PS. The upstream regulation of MTA1 expression during meiosis by endogenous EGF was further confirmed in vitro using a selective Epidermal Growth Factor Receptor (EGFR) inhibitor, tryphostin AG1478. Moreover, inhibition of EGF signaling by AG1478 treatment significantly suppressed the heat stress-induced MTA1 in PS. The available data collectively suggest that EGF signaling regulates the expression of MTA1 in PS, and early activation of EGF/MTA1 cascade in PS in response to heat stress may serve as an intrinsic self-defensive mechanism maintaining apoptotic balance during meiotic heat stress.","PeriodicalId":15029,"journal":{"name":"Journal of andrology","volume":"2 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2013-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70783856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2013-11-27DOI: 10.4172/2167-0250.1000112
M. Dashti, Afaf Y Alhamar, H. Shawky, M. Bakhiet
Objective: Current information on using anti-estrogenic compounds, antioxidant vitamins and minerals in treatment of male factor infertility still remains controversial. Herein, we investigated the pregnancy outcome in male factor infertile patients using a combination of non-specific empiric modalities and Intra Uterine Insemination (IUI) procedures. Subjects and Methods: The study involved a group of 33 infertile couples with mild male factor infertility who previously failed two IUI attempts. The patients received tamoxifen, vitamin E, zinc, and selenium for three months prior to their third IUI treatment cycle. Four important parameters were mainly noted: sperm concentration, motility, forward progression and the percentage normal forms. Results: There was no difference between these parameters in semen samples of our study group in the first and second IUI treatment cycles (p<0.96, p<0.23, p<0.59, p<0.84 respectively). However, after completion of the empiric therapy course and in the third IUI treatment cycle, significant differences in overall values for the four semen parameters were detected in comparison to the earlier two IUI cycles (range p<0.005 to p<0.0005), except for semen volume and sperm normal forms, resulting in a chemical pregnancy rate of 30.3%, a clinical pregnancy rate of 21.2% and a delivery rate of 18.1%. Grouping the female patients according to the Body Mass Index (BMI) showed imperative differences in pregnancy outcome, yet there was no clear effect of age over pregnancy success rates in our study group. Conclusion: Combined empirical therapies can improve semen parameters in infertile men with mild male factor. Double insemination procedures with improved semen samples, can contribute in increasing the chances of pregnancy and life birth more significantly in females with lower BMI.
{"title":"Effectiveness of Combined Empirical Therapies and Double IUI Procedures in Treatment of Male Factor Infertility","authors":"M. Dashti, Afaf Y Alhamar, H. Shawky, M. Bakhiet","doi":"10.4172/2167-0250.1000112","DOIUrl":"https://doi.org/10.4172/2167-0250.1000112","url":null,"abstract":"Objective: Current information on using anti-estrogenic compounds, antioxidant vitamins and minerals in treatment of male factor infertility still remains controversial. Herein, we investigated the pregnancy outcome in male factor infertile patients using a combination of non-specific empiric modalities and Intra Uterine Insemination (IUI) procedures. \u0000Subjects and Methods: The study involved a group of 33 infertile couples with mild male factor infertility who previously failed two IUI attempts. The patients received tamoxifen, vitamin E, zinc, and selenium for three months prior to their third IUI treatment cycle. Four important parameters were mainly noted: sperm concentration, motility, forward progression and the percentage normal forms. \u0000Results: There was no difference between these parameters in semen samples of our study group in the first and second IUI treatment cycles (p<0.96, p<0.23, p<0.59, p<0.84 respectively). However, after completion of the empiric therapy course and in the third IUI treatment cycle, significant differences in overall values for the four semen parameters were detected in comparison to the earlier two IUI cycles (range p<0.005 to p<0.0005), except for semen volume and sperm normal forms, resulting in a chemical pregnancy rate of 30.3%, a clinical pregnancy rate of 21.2% and a delivery rate of 18.1%. Grouping the female patients according to the Body Mass Index (BMI) showed imperative differences in pregnancy outcome, yet there was no clear effect of age over pregnancy success rates in our study group. \u0000Conclusion: Combined empirical therapies can improve semen parameters in infertile men with mild male factor. Double insemination procedures with improved semen samples, can contribute in increasing the chances of pregnancy and life birth more significantly in females with lower BMI.","PeriodicalId":15029,"journal":{"name":"Journal of andrology","volume":"2013 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2013-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70783877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：