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Effect of Applying Clove and Cinnamon Essential Oils to Milk Rice Pudding in Controlling Bacillus cereus and Bacillus subtilis Growth with Respect to the Sensory Traits 在牛奶米布丁中添加丁香和肉桂精油对控制蜡样芽孢杆菌和枯草芽孢杆菌生长及感官特征的影响
Pub Date : 2024-06-09 DOI: 10.21608/javs.2024.285311.1333
Aml S. Ibrahim, O. W. Hegab, Neveen S.M. Soliman
Milk rice pudding (MRP) is a commercial and popular dairy dessert, but owing to its characteristics and valuable ingredients, it may be contaminated by many pathogenic and spoilage microorganisms. So, this study aimed to improve the quality and safety of MRP by using cinnamon and clove essential oils. Concerning the evaluation of the minimum inhibitory concentration (MIC) for both oils with 0.2, 0.5, and 1% concentrations, B. cereus and B. subtilis were sensitive (+) to cinnamon and clove 0.5%, with inhibition zones of 13.3 and 14 mm for cinnamon and 11.3 and 12 mm for clove EO, respectively. While both bacteria were very sensitive (++) to cinnamon 1% (18.8 and 19.5 mm) and clove 1% (17.3 and 18.7 mm), respectively. Therefore, MRP was prepared by adding cinnamon and clove EOs at 0.6%. Treatments containing EOs showed a significant reduction of tested microorganisms compared to controls. B. cereus wasn’t detected in clove and cinnamon EO treatments at day 21 of the storage period, while B. subtilis vanished on day 14 for the cinnamon treatment and on day 21 for clove MRP. Moreover, the results revealed the enhancement of sensory characteristics of MRP supplemented with EOs without any significant alteration in their pH values. This study recommends the addition of cinnamon and clove EOs (0.6%) to MRP, as it isn’t only an excellent substitution of chemical preservatives with powerful antibacterial efficiency but also improves the overall acceptance of the product.
牛奶米布丁(MRP)是一种商业化的流行乳品甜点,但由于其特点和珍贵成分,可能会受到许多致病和腐败微生物的污染。因此,本研究旨在通过使用肉桂和丁香精油来提高麦乳精的质量和安全性。关于两种精油 0.2、0.5 和 1%浓度的最低抑菌浓度(MIC)评估,蜡样芽孢杆菌和枯草芽孢杆菌对肉桂和丁香 0.5%的浓度敏感(+),肉桂和丁香环氧乙烷的抑菌区分别为 13.3 毫米和 14 毫米,丁香环氧乙烷的抑菌区分别为 11.3 毫米和 12 毫米。而这两种细菌对肉桂 1%(18.8 和 19.5 毫米)和丁香 1%(17.3 和 18.7 毫米)非常敏感(++)。因此,在制备 MRP 时添加了 0.6% 的肉桂和丁香环氧乙烷。与对照组相比,含有环氧乙烷的处理明显减少了测试微生物的数量。在贮藏期的第 21 天,丁香和肉桂环氧乙烷处理中未检测到蜡样芽孢杆菌,而肉桂处理中的枯草芽孢杆菌在第 14 天消失,丁香 MRP 中的枯草芽孢杆菌在第 21 天消失。此外,研究结果表明,添加了环氧乙烷的 MRP 感官特征得到了改善,但其 pH 值没有发生任何显著变化。本研究建议在 MRP 中添加肉桂和丁香环氧乙烷(0.6%),因为它不仅能很好地替代具有强大抗菌功效的化学防腐剂,还能提高产品的整体接受度。
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引用次数: 0
Effect of Different Fractions of Lawsonia inermis Linn on Haematobiochemical Changes, Osmotic Fragility and Lipid Profile in Streptozotocin-induced Diabetic Wistar Rats 不同提取物对链脲佐菌素诱导的糖尿病 Wistar 大鼠血液生化变化、渗透脆性和血脂谱的影响
Pub Date : 2024-05-07 DOI: 10.21608/javs.2024.276758.1328
A. Aremu, O. Oridupa, Norazah Bashar
Diabetes mellitus is a major health challenge that has harmful effects on the quality of life globally as a result of its numerous complications. This study aimed to evaluate the positive modulatory effect of Lawsonia inermis (LI) Linn on various haemobiochemical parameters and lipid profiles in streptozotocin-induced diabetic rats. Ten groups of diabetic rats (n = 5) were orally administered 50 and 100 mg/kg of three different fractions of Lawsonia inermis : metformin (500 mg/kg) and glibenclamide (5 mg/kg), whereas untreated hyperglycaemic and normoglycaemic rats received distilled water. The results showed increased red blood cells (RBC) in treated rats compared to untreated diabetic rats. Hb, MCV, MCH, and MCHC decreased nonsignificantly, whereas WBC increased nonsignificantly. Neutrophil increased non-significantly in diabetic, untreated rats, while all the treatment groups decreased non-significantly. Lymphocytes and monocytes increased non-significantly (p > 0.05). Most treatment groups showed a non-significant (p > 0.05) increase in the platelet count. The ALT levels decreased non-significantly (p > 0.05) compared to normoglycemic rats. The AST levels decreased significantly (p<0.01). ALP decreased non-significantly in both treated and untreated groups, whereas bilirubin did not show any significant changes. Creatinine and urea levels in untreated diabetic rats increased non-significantly, while treatment groups decreased non-significantly. Lawsonia inermis -treated rats showed significant improvement in erythrocytic fragility, while glycated haemoglobin in untreated-diabetic and glibenclamide-treated rats increased significantly (p > 0.001). Triglycerides and high-density lipoprotein decreased non-significantly, while low-density lipoprotein increased non-significantly (p > 0.05) in diabetic untreated rats. All treatment groups showed a non-significant (P > 0.05) decrease in low-density lipoprotein. Lawsonia inermis showed a significant positive modulatory effect on the haemobiochemical changes, glycated Hb, osmotic fragility, and lipid profile in streptozotocin-induced diabetic rats.
糖尿病是一项重大的健康挑战,其众多并发症对全球的生活质量造成了有害影响。本研究旨在评估Lawsonia inermis (LI) Linn对链脲佐菌素诱导的糖尿病大鼠的各种血液生化指标和血脂谱的积极调节作用。给 10 组糖尿病大鼠(n = 5)口服 50 和 100 毫克/千克的三种不同的茵陈罗汉果萃取物:二甲双胍(500 毫克/千克)和格列本脲(5 毫克/千克),而给未经处理的高血糖大鼠和正常血糖大鼠口服蒸馏水。结果显示,与未治疗的糖尿病大鼠相比,治疗大鼠的红细胞(RBC)增加了。血红蛋白(Hb)、血浆容积(MCV)、血红蛋白胆固醇(MCH)和血红蛋白胆固醇胆固醇(MCHC)均无明显减少,而白细胞(WBC)则无明显增加。未治疗的糖尿病大鼠的中性粒细胞增加不明显,而所有治疗组大鼠的中性粒细胞减少不明显。淋巴细胞和单核细胞增加无显著性差异(P > 0.05)。大多数治疗组的血小板计数增加不明显(p > 0.05)。与正常血糖大鼠相比,ALT 水平下降不明显(p > 0.05)。谷草转氨酶水平明显下降(p 0.001)。未经治疗的糖尿病大鼠的甘油三酯和高密度脂蛋白下降不明显,而低密度脂蛋白上升不明显(P > 0.05)。所有治疗组的低密度脂蛋白下降均不显著(P > 0.05)。茵陈对链脲佐菌素诱导的糖尿病大鼠的血液生化变化、糖化血红蛋白、渗透脆性和血脂谱有显著的积极调节作用。
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引用次数: 0
Gangrene of the Tongue Develops 48 Hours Following Ovariohysterectomy as a Treatment of Open-Cervix Pyometra in a Bitch 治疗母犬开放性子宫颈脓肿的卵巢切除术后 48 小时出现舌头坏疽
Pub Date : 2024-05-07 DOI: 10.21608/javs.2024.280576.1329
Ashraf Abu-Seida
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引用次数: 0
Detection of clfA, clfB and coa genes in Methicillin-Resistant Staphylococcus aureus (MRSA) isolated from Nasal Cavity of Cows, Buffalo and their Breeders in Nineveh Governorate, Iaq 检测从伊拉克尼尼微省奶牛、水牛及其饲养者鼻腔中分离出的耐甲氧西林金黄色葡萄球菌(MRSA)的 clfA、clfB 和 coa 基因
Pub Date : 2024-05-06 DOI: 10.21608/javs.2024.276537.1324
H. Rahma, Dh. Jwher
The present study aimed to isolate and identify Methicillin-Resistant Staphylococcus aureus MRSA from the nasal cavity of healthy cows and buffaloes and their breeders in Nineveh Governorate and detect some virulence factors by using molecular methods. A total of 150 samples of cotton swabs were collected randomly from different areas of Nineveh governorate. The samples were cotton swabs from the nasal passages of healthy cattle, buffaloes, and their breeders (50 swabs of each type). All the samples were subjected to culture and molecular testing. The results showed the highest isolation percentage of S. aureus from cattle followed by breeders, then buffaloes, at 54%, 40%, and 32%, respectively. The total isolation percentage of MRSA was 65.1%. The highest percentage was in buffaloes, followed by breeders and cattle, at 93.75%, 70%, and 44.44%, respectively. Out of 41 isolates from cattle, buffaloes, and their breeders, the virulence genes clf A, clf B, and coa were detected in MRSA at rates of 100%, 80.49%, and 65.85%, respectively. The current study concluded that cattle and buffalo are considered carriers and potential transmitters of MRSA, which makes them risk factors for infection in humans, especially those who are in direct contact with animals. Together, these findings also highlight the need to prevent the transmission of zoonotic pathogens to humans via occupational exposure or consumption of contaminated animal products.
本研究旨在从尼尼微省健康奶牛和水牛及其饲养者的鼻腔中分离和鉴定耐甲氧西林金黄色葡萄球菌 MRSA,并采用分子方法检测一些毒力因子。研究人员从尼尼微省的不同地区随机采集了 150 份棉拭子样本。这些棉拭子样本来自健康牛、水牛及其饲养者的鼻腔(每种类型各 50 个棉拭子样本)。所有样本都进行了培养和分子检测。结果显示,牛的金黄色葡萄球菌分离率最高,其次是种牛,然后是水牛,分别为 54%、40% 和 32%。MRSA 的总分离率为 65.1%。水牛的比例最高,其次是种牛和牛,分别为 93.75%、70% 和 44.44%。在来自牛、水牛及其种牛的 41 个分离物中,MRSA 的毒力基因 clf A、clf B 和 coa 的检出率分别为 100%、80.49% 和 65.85%。目前的研究认为,牛和水牛被认为是 MRSA 的携带者和潜在传播者,这使它们成为人类感染的危险因素,尤其是那些与动物直接接触的人。这些研究结果也突出表明,有必要防止人畜共患病病原体通过职业接触或食用受污染的动物产品传播给人类。
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引用次数: 0
Effect of Obesity on Awakening Time after Isoflurane/Sevoflurane Inhalation Anesthesia in Dogs 肥胖对狗吸入异氟醚/塞氟醚麻醉后苏醒时间的影响
Pub Date : 2024-03-17 DOI: 10.21608/javs.2024.266563.1311
Masao Arisato, Shozo Okano
To elucidate the relationship between obesity, awakening time, and anesthesia in dogs, we conducted a comparative analysis of the awakening time following isoflurane and sevoflurane anesthesia in both standard-weight and obese groups. Six female beagle dogs within the standard weight range underwent 1-hour inhalation anesthesia with either isoflurane or sevoflurane. We recorded the time of extubation, head lift, and standing. Additionally, a parallel experiment was conducted on the same test animals after inducing obesity through nutritional management, with criteria for obesity defined as a body conditioning score of 4 or higher and a body fat percentage of 30% or higher. Under isoflurane anesthesia, we observed a tendency for a longer awakening time in the obese group compared to the standard weight group. However, this trend was not evident with sevoflurane anesthesia. Furthermore, when comparing awakening time with both anesthetics, no significant difference was found between the standard weight groups. In contrast, within the obese group, awakening time was significantly prolonged with isoflurane anesthesia compared to sevoflurane anesthesia. These results confirm that obesity contributes to delay awakening in dogs. Additionally, in the anesthesia management of obese dogs, the use of sevoflurane is anticipated to reduce awakening time compared to isoflurane.
为了阐明狗的肥胖、苏醒时间和麻醉之间的关系,我们对标准体重组和肥胖组的异氟醚和七氟醚麻醉后的苏醒时间进行了比较分析。六只标准体重范围内的雌性小猎犬接受了 1 小时的异氟醚或七氟醚吸入麻醉。我们记录了拔管、抬头和站立的时间。此外,在通过营养管理诱导肥胖后,我们对相同的实验动物进行了平行实验,肥胖的标准是体质评分达到或超过 4 分,体脂率达到或超过 30%。在异氟烷麻醉下,我们观察到肥胖组的苏醒时间往往比标准体重组更长。然而,这种趋势在七氟醚麻醉下并不明显。此外,在比较两种麻醉剂的苏醒时间时,标准体重组之间没有发现明显差异。相反,在肥胖组中,与七氟醚麻醉相比,异氟醚麻醉的苏醒时间明显延长。这些结果证实,肥胖会导致狗的苏醒时间延迟。此外,在对肥胖犬进行麻醉管理时,与异氟醚相比,使用七氟醚有望缩短苏醒时间。
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引用次数: 0
Impact of Fabricated Coral Shell Hydroxyapatite Powder and Autologous Plasma Rich- fibrin in Remodeling of the Mandibular Bone Critical Size Defect in Dogs: Histopathological and Immunohistochemical Study 珊瑚贝壳羟基磷灰石粉和自体血浆富纤维蛋白在重塑犬下颌骨临界尺寸缺陷中的影响:组织病理学和免疫组化研究
Pub Date : 2024-03-10 DOI: 10.21608/javs.2024.266431.1312
A. Atiyah, Alkattan L.M.
Histopathological and immunohistochemical assessment of fabricated coral shell hydroxyapatite (CSHA) and plasma rich fibrin (PRF) in remodeling of the induced critical size defect of the mandibular bone in the dogs: Twenty-seven adult dogs of both sexes were included and equally divided into three equal groups: control, plasma-rich fibrin (PRF) and hydroxyapatite group (CSHA). The experimental mandibular bone defect was induced in a circular shape, and the dimensions of the defect were 14×5mm. Evaluation of the healing progress of the defect and associated macroscopical, histopathological, and Immunohistological findings was recorded in all studied groups at 7, 15, and 30 days post-operatively. Macroscopically, the healing was evaluated by the presence of new bone tissue filling the bone gap defect in all groups during different follow-up periods. In the plasma-rich fibrin (PRF) group, the gap was highly filled with hard, firm tissues that filled all borders and the centre of the induced gap in comparison with the coral shell hydroxyl apatite group (CSHA), which is partially filled with hard tissue. Histopathologically, the progress of healing in the PRF group was represented by the presence of highly mature connective tissue and new woven bone formation at seven days and well-developed mature bone inside defective bone at 15 and 30 days post-operatively, whereas in the CSHA group, the results were represented by the occlusion of highly mature connective tissue and new woven bone formation inside the induced hole at 15 and 30 days post-operatively. At 30 days post-surgery, in the control group, there was the presence of newly formed woven bone surrounded by the edge of the mandible bone. The immunohistochemical expression of the alkaline phosphatase (ALP) in the mandible bone at 30 days PS in the control group was represented by weak positive expression, while mild positive expression was indicated in the CSHA group and moderate positive expression in the PRF group. In conclusion, this research exhibited the role of both CSHA and PRF in improving the healing process of defective mandible bones, with a clear superiority of the beneficial value of using PRF. The histopathological and immunohistochemistry assessments emphasize these results.
对制备的珊瑚贝壳羟基磷灰石(CSHA)和富血浆纤维蛋白(PRF)在诱导狗下颌骨临界大小缺损重塑中的作用进行组织病理学和免疫组化评估:将 27 只成年雌雄犬平均分为三组:对照组、富血浆纤维蛋白组(PRF)和羟基磷灰石组(CSHA)。实验组下颌骨缺损呈圆形,缺损尺寸为 14×5mm。在术后 7 天、15 天和 30 天,对所有研究组的缺损愈合进展以及相关的宏观、组织病理学和免疫组织学结果进行评估。在不同的随访期内,所有研究组的愈合情况都是通过新骨组织填充骨间隙缺损来评估的。在富含血浆纤维蛋白(PRF)组中,间隙高度填充了坚硬的组织,填充了诱导间隙的所有边界和中心,而在珊瑚贝壳羟基磷灰石(CSHA)组中,间隙部分填充了坚硬的组织。从组织病理学角度看,PRF 组的愈合进展表现为术后 7 天出现高度成熟的结缔组织和新的骨编织,术后 15 天和 30 天缺损骨内出现发育良好的成熟骨,而 CSHA 组的结果则表现为术后 15 天和 30 天高度成熟的结缔组织闭塞,诱导孔内有新的骨编织形成。对照组在术后 30 天时,下颌骨边缘出现了新形成的编织骨。对照组术后 30 天下颌骨中碱性磷酸酶(ALP)的免疫组化表达为弱阳性,CSHA 组为轻度阳性,PRF 组为中度阳性。总之,本研究显示了 CSHA 和 PRF 在改善下颌骨缺损愈合过程中的作用,其中 PRF 的有益价值明显高于 CSHA。组织病理学和免疫组化评估结果都强调了这一点。
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引用次数: 0
Retrospective Study of Canine Pathologies Causing Mortalities in Ilorin Kwara State, Nigeria 尼日利亚伊洛林-夸拉州导致死亡的犬类疾病回顾性研究
Pub Date : 2024-03-07 DOI: 10.21608/javs.2024.262746.1309
Mohammed Adam, A. Ajadi, J. Atata, Olatunde David Akanbi, S. Shoyinka, Muhammad Abubakar, Moshood Olaniyi
Canine pathologies refer to the various diseases, disorders, and abnormalities that can affect a dog’s different body systems and cause mortality. The primary aim of this study was to investigate the common pathologies that are associated with the mortality of dogs in Ilorin, Kwara State. A retrospective review of post-mortem records from 7 years was undertaken at a necropsy unit of the Department of Veterinary Pathology, University of Ilorin. Data collected included age, sex, breed, disease pathogens, and year. A total of 150 dog carcasses were presented for necropsy during the period between January 2016 and December 2022. In this study, the cases used were confirmed based on PCR results in some cases, bacterial culture and isolation, parasite identification, gross lesions, and histopathological findings in other cases. Descriptive statistics were employed to evaluate the effect of disease pathogens on age, sex, and breed distribution patterns associated with the mortality of dogs. The prevalence of viral infection among other diseases was statistically significant (34.00%; OR = 0.53; P<0.01). The percentage prevalence of each disease showed that bacterial infections were evident, with Staphylococcosis being the most prevalent at 49.01%, followed by Salmonellosis at 7.84%, Leptospirosis at 23.53%, and Brucellosis at 19.6%. Parasitic infections were also notable, encompassing canine babesiosis (34.80%), acariosis (39.10%), and canine ehrlichiosis (26.08%). Toxicosis cases were largely dominated by diclovous poisoning, constituting 88.88% of instances, while medication toxicity was observed in 11.11% of cases. Neoplastic conditions were represented by hepatic lymphoma, with a prevalence of 1%, while nutritional deficiencies included hepatic lipidosis, noted in 1% of cases. Traumatic injuries were common, with bone fracture having the highest prevalence at 66.67% and muscle laceration observed in 33.33% of cases. Intestinal obstruction cases, specifically gastric impaction, were noted in 1% of cases. Viral infections comprised parvovirus infection with the highest prevalence at 81%, and canine distemper was observed in 18.92% of cases. In conclusion, this study has exposed the leading causes of mortalities among the dog population, which include parvovirus enteritis, staphylococcosis, and acariosis infections. The emphasis should be on preventive measures to curtail disease transmission and associated financial loss.
犬病理学是指可能影响犬不同身体系统并导致死亡的各种疾病、失调和异常。本研究的主要目的是调查与夸拉州伊洛林市犬只死亡有关的常见病症。研究人员在伊洛林大学兽医病理学系的尸体解剖室对 7 年来的尸检记录进行了回顾性审查。收集的数据包括年龄、性别、品种、疾病病原体和年份。在 2016 年 1 月至 2022 年 12 月期间,共有 150 具狗尸体被送去尸检。在本研究中,部分病例是根据 PCR 结果确认的,其他病例则是根据细菌培养和分离、寄生虫鉴定、大体病变和组织病理学结果确认的。研究采用了描述性统计方法,以评估疾病病原体对与狗的死亡率相关的年龄、性别和品种分布模式的影响。在其他疾病中,病毒感染的发病率具有显著的统计学意义(34.00%;OR = 0.53;P<0.01)。各种疾病的发病率百分比显示,细菌感染非常明显,其中葡萄球菌病的发病率最高,为 49.01%,其次是沙门氏菌病(7.84%)、钩端螺旋体病(23.53%)和布鲁氏菌病(19.6%)。寄生虫感染也很常见,包括犬巴贝斯虫病(34.80%)、犬吸虫病(39.10%)和犬埃立克病(26.08%)。中毒病例主要是二氯甲烷中毒,占 88.88%,药物中毒占 11.11%。肿瘤性疾病以肝淋巴瘤为代表,发病率为 1%,营养缺乏包括肝脂质沉着病,发病率为 1%。外伤很常见,其中骨折的发病率最高,为 66.67%,肌肉裂伤占 33.33%。1%的病例出现肠梗阻,特别是胃嵌塞。病毒感染包括副病毒感染,发病率最高,占 81%,犬瘟热占 18.92%。总之,这项研究揭示了导致犬只死亡的主要原因,其中包括副病毒性肠炎、葡萄球菌病和螨虫感染。重点应放在预防措施上,以减少疾病传播和相关的经济损失。
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引用次数: 0
Pharmacodynamic and Pharmacokinetic Comparison between Selective and Non-selective COX-2 Inhibitors in Mice 选择性和非选择性 COX-2 抑制剂在小鼠体内的药效学和药代动力学比较
Pub Date : 2024-03-07 DOI: 10.21608/javs.2024.266445.1310
Taimaa Yahya, Yaareb Mousa
Nowadays, there is a need for good and efficacious NSAIDs with minimal side effects to be applied in veterinary medicine. The aim was to compare the pharmacodynamics (analgesia and inhibition of COX-2) and pharmacokinetics between selective (nimesulide) and non-selective (aspirin) COX-2 inhibitors in mice. Assessing the median effective doses by using the up-and-down method, COX-2 activity and plasma concentrations for both nimesulide and aspirin with their pharmacokinetic profiles in mice. The median effective doses (ED50s) of nimesulide and aspirin were found to be 7.9 and 212.23 mg/kg, respectively, using the hot-plate. Both nimesulide (15.8 mg/kg, i.m.) and aspirin (424.5 mg/kg, i.m.) inhibited COX-2 activity through a decrease in COX-2 concentrations in the plasma, liver, and kidney of mice, with superior inhibition when administering nimesulide in comparison to the control (negative and positive) and aspirin-treated groups. Plasma concentrations of nimesulide (15.8 mg/kg, i.m.) measured for different comparable periods of 0.5, 1, 2, 4, and 24 hours were higher than those of aspirin, which were 14.62, 9.22, 9.88, 7.38 and 2.27 µg/ml, respectively, while aspirin (424.5 mg/kg, i.m.) was 4.35, 3.17, 2.54, 2.25 and 1.21 µg/ml, at the same measured times. Nimesulide pharmacokinetic variables were estimated to be AUC0-∞ 169.18, AUMC0-∞ 2358,72, Kel 0.06, Cmax 14.62, Tmax 0.5, t1/2β 11.07, MRT 13.94, Vss 1.49, and Cl 0.09, while aspirin pharmacokinetic parameters differed to be 82.31, 2428.32, 0.03, 4.35, 0.5, 21.25, 158.12, and 5.16, respectively. The study concluded that nimesulide has superior pharmacological properties (analgesic, antipyretic, and anti-inflammatory) than aspirin due to its ability to inhibit COX-2 more selectively and its unique pharmacokinetics in mice, which may be useful in veterinary medicine.
如今,兽医领域需要副作用小、疗效好的非甾体抗炎药。本研究旨在比较小鼠体内选择性(尼美舒利)和非选择性(阿司匹林)COX-2 抑制剂的药效学(镇痛和对 COX-2 的抑制)和药代动力学。采用上下法评估中位有效剂量、尼美舒利和阿司匹林的 COX-2 活性和血浆浓度及其在小鼠体内的药代动力学特征。通过热板法发现,尼美舒利和阿司匹林的中位有效剂量(ED50s)分别为 7.9 毫克/千克和 212.23 毫克/千克。尼美舒利(15.8 毫克/千克,静注)和阿司匹林(424.5 毫克/千克,静注)都能通过降低小鼠血浆、肝脏和肾脏中 COX-2 的浓度来抑制 COX-2 的活性,与对照组(阴性和阳性)和阿司匹林处理组相比,尼美舒利的抑制作用更强。尼美舒利(15.8 毫克/千克,静注)在 0.5、1、2、4 和 24 小时不同可比时间段的血浆浓度分别为 14.62、9.22、9.88、7.38 和 2.27 微克/毫升,高于阿司匹林,而阿司匹林(424.5 毫克/千克,静注)在相同测量时间段的血浆浓度分别为 4.35、3.17、2.54、2.25 和 1.21 微克/毫升。尼美舒利的药代动力学变量估计为:AUC0-∞ 169.18、AUMC0-∞ 2358、72、Kel 0.06、Cmax 14.62、Tmax 0.5、t1/2β 11.07、MRT 13.94、Vss 1.49、Cl 0.09,而阿司匹林药代动力学参数差异分别为 82.31、2428.32、0.03、4.35、0.5、21.25、158.12、5.16。研究认为,尼美舒利具有比阿司匹林更优越的药理特性(镇痛、解热和抗炎),这是因为尼美舒利能够更有选择性地抑制 COX-2,并且在小鼠体内具有独特的药代动力学,这可能会在兽医学中发挥作用。
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引用次数: 0
Haemato-biochemical Response to Kirschner Pin and Improvised Chrome Vanadium Long Screw Used for the Stabilization of Femoral Fracture in Goats 用于稳定山羊股骨骨折的克氏针和改良铬钒长螺钉的血液生化反应
Pub Date : 2024-03-06 DOI: 10.21608/javs.2024.262263.1307
Ahmad Salisu, Abubakar Adamu Abdul, Buhari Salisu, A. Salisu, Yakubu Abubakar Sadiq, Peter Charles
The stainless-steel orthopaedic implants used for the management of various types of fracture internal fixations are very expensive; this makes their utilization in food animals relatively noneconomical. There is a need to have an improvised implant that is cheaper and available for use in food animals to manage complicated fractures requiring open reduction and internal fixation. The objective of this study is to evaluate the haematological and biochemical changes following the reduction of stable femoral fractures in goats using conventional (Kirschner pin) and improvised chrome vanadium-coated long crews. Twelve apparently healthy Red Sokoto bucks were randomly divided into two groups of six (n=6) were used for this study. A transverse femoral diaphyseal fracture was created using orthopaedic wire in both groups. In group A, a conventional Kirschner pin size 4.0x125 mm 2 , single trocar, non-threaded, was used to immobilize the fracture, while in group B, an improvised test chrome vanadium (long screw) size 2.3 mm was used for the fracture immobilizations. Blood samples were collected before fracture induction at the base line, serving as a control. The haematological and biochemical assessments were performed at 0 weeks (immediate postoperative) and subsequently at 2, 4, 6, 8, 10 and 12 weeks postoperatively. Packed cell volume (PCV%), haemoglobin concentration (Hb), red blood cell count (RBCs), white blood cell count (WBCs) and differential leukocytic count (neutrophils, lymphocytes and monocytes) were evaluated. Also, serum activity of alkaline phosphatase (ALP), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) was detected. Besides, serum calcium, phosphorous and creatinine levels were evaluated. There were no significant differences between the two groups in PCV, haemoglobin, RBCs, WBCs and neutrophils, and the values were within the normal range. However, lymphocytes and monocytes were significantly (p<0.05) different at weeks 2 and 6, respectively. Biochemical parameters revealed significant (p<0.05) changes in serum ALT (weeks 0, 2, 4), ALP (week 6), creatinine (weeks 10 and 12), and calcium (week 2) at some postoperative intervals. However, no variations were observed in serum AST and phosphorous, which were within the normal range. The improvised chrome vanadium (Long screw) can be used safely in goats for the management of stable femoral fractures without significant adverse changes to hemato-biochemical profiles within twelve weeks.
用于治疗各种骨折内固定的不锈钢骨科植入物非常昂贵,因此在食用动物中使用这些植入物相对不划算。因此,有必要开发一种更便宜的简易植入物,供食用动物用于处理需要切开复位和内固定的复杂骨折。本研究的目的是评估使用传统(Kirschner 针)和简易铬钒涂层长套管对山羊稳定股骨骨折进行复位后的血液学和生化变化。本研究使用了 12 只明显健康的红色索科托公山羊,随机分为两组,每组 6 只(n=6)。两组均使用矫形钢丝造成股骨二骺横向骨折。A 组使用尺寸为 4.0x125 mm 2、单套管、无螺纹的传统克氏针固定骨折,B 组使用尺寸为 2.3 mm 的简易试验铬钒(长螺钉)固定骨折。骨折诱导前在基线处采集血样作为对照。在术后 0 周(术后即刻)以及术后 2、4、6、8、10 和 12 周进行血液和生化评估。评估的指标包括包装细胞容积(PCV%)、血红蛋白浓度(Hb)、红细胞计数(RBCs)、白细胞计数(WBCs)和白细胞差异计数(中性粒细胞、淋巴细胞和单核细胞)。此外,还检测了血清碱性磷酸酶(ALP)、丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)的活性。此外,还评估了血清钙、磷和肌酐水平。两组患者的 PCV、血红蛋白、红细胞、白细胞和中性粒细胞无明显差异,均在正常范围内。然而,淋巴细胞和单核细胞在第 2 周和第 6 周分别有显著差异(p<0.05)。生化指标显示,在术后的一些间隔期,血清谷丙转氨酶(第 0、2、4 周)、谷草转氨酶(第 6 周)、肌酐(第 10 和 12 周)和血钙(第 2 周)均有明显变化(p<0.05)。不过,血清谷草转氨酶和磷没有变化,均在正常范围内。简易铬钒(长螺钉)可安全地用于山羊稳定型股骨骨折的治疗,在十二周内不会对血液生化指标产生明显的不良变化。
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引用次数: 0
A mini-review on fish mass kills within the Egyptian fisheries and aquaculture sectors: Impacts and proposed solutions 关于埃及渔业和水产养殖业鱼类大量死亡的小型回顾:影响和拟议解决方案
Pub Date : 2024-02-24 DOI: 10.21608/javs.2024.262508.1308
A. E. Eissa
Mass fish kills are abrupt events in which a significant number of fish of different ages and species perish in a specific aquatic region. Dramatic waves of mass kills have involved a large variety of economic fish species in both Egyptian fisheries and the aquaculture sector. Several episodes of mass mortalities of various infectious and noninfectious aetiologies have targeted a wide spectrum of freshwater and marine fish in both open-water and captive environments. Kafr El-Sheikh, Sharkia, Dakahlia, Fayoum, Alexandria and Giza were the most affected Egyptian provinces. The Mediterranean basin, Lake Manzala, Lake Mariott, Lake Burullus, River Nile, and some provincial water streams and municipal water drains were all reported to have had one or more episodes of mass kills over the past two decades. These mass kills were mainly attributed to different types / magnitudes of aquatic environmental pollution, which was explicitly aggravated by poor aquaculture / fishing practices. Further, the frequent absence of veterinary guidance has had a great negative impact on providing possible solutions for this disaster in the near future. Ultimately, adopting Good Aquaculture Practice (GAP), competent biosecurity strategies, and regular veterinary supervision will be the most practical solutions that ensure the reliable growth and sustainability of both Egyptian fisheries and aquaculture sectors.
鱼类大量死亡是指在一个特定的水域中,大量不同年龄和种类的鱼类死亡的突发事件。在埃及渔业和水产养殖业中,各种经济鱼类都曾出现过大规模死亡的现象。在开放水域和人工饲养环境中,各种感染性和非感染性病原体导致的大规模死亡事件已针对多种淡水鱼和海水鱼。Kafr El-Sheikh、Sharkia、Dakahlia、Fayoum、Alexandria 和 Giza 是埃及受影响最严重的省份。据报告,地中海盆地、曼扎拉湖、马里奥特湖、布鲁鲁斯湖、尼罗河以及一些省份的溪流和市政排水沟在过去二十年里都发生过一次或多次大规模死亡事件。这些大规模死亡事件主要归因于不同类型/程度的水生环境污染,而不良的水产养殖/捕鱼方式则明显加剧了污染。此外,兽医指导的频繁缺失也对在不久的将来为这场灾难提供可能的解决方案产生了极大的负面影响。最终,采用良好水产养殖规范 (GAP)、有效的生物安全策略和定期的兽医监督将是确保埃及渔业和水产养殖业可靠增长和可持续发展的最切实可行的解决方案。
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引用次数: 0
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Journal of Applied Veterinary Sciences
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