Pub Date : 2024-06-09DOI: 10.21608/javs.2024.285311.1333
Aml S. Ibrahim, O. W. Hegab, Neveen S.M. Soliman
Milk rice pudding (MRP) is a commercial and popular dairy dessert, but owing to its characteristics and valuable ingredients, it may be contaminated by many pathogenic and spoilage microorganisms. So, this study aimed to improve the quality and safety of MRP by using cinnamon and clove essential oils. Concerning the evaluation of the minimum inhibitory concentration (MIC) for both oils with 0.2, 0.5, and 1% concentrations, B. cereus and B. subtilis were sensitive (+) to cinnamon and clove 0.5%, with inhibition zones of 13.3 and 14 mm for cinnamon and 11.3 and 12 mm for clove EO, respectively. While both bacteria were very sensitive (++) to cinnamon 1% (18.8 and 19.5 mm) and clove 1% (17.3 and 18.7 mm), respectively. Therefore, MRP was prepared by adding cinnamon and clove EOs at 0.6%. Treatments containing EOs showed a significant reduction of tested microorganisms compared to controls. B. cereus wasn’t detected in clove and cinnamon EO treatments at day 21 of the storage period, while B. subtilis vanished on day 14 for the cinnamon treatment and on day 21 for clove MRP. Moreover, the results revealed the enhancement of sensory characteristics of MRP supplemented with EOs without any significant alteration in their pH values. This study recommends the addition of cinnamon and clove EOs (0.6%) to MRP, as it isn’t only an excellent substitution of chemical preservatives with powerful antibacterial efficiency but also improves the overall acceptance of the product.
{"title":"Effect of Applying Clove and Cinnamon Essential Oils to Milk Rice Pudding in Controlling Bacillus cereus and Bacillus subtilis Growth with Respect to the Sensory Traits","authors":"Aml S. Ibrahim, O. W. Hegab, Neveen S.M. Soliman","doi":"10.21608/javs.2024.285311.1333","DOIUrl":"https://doi.org/10.21608/javs.2024.285311.1333","url":null,"abstract":"Milk rice pudding (MRP) is a commercial and popular dairy dessert, but owing to its characteristics and valuable ingredients, it may be contaminated by many pathogenic and spoilage microorganisms. So, this study aimed to improve the quality and safety of MRP by using cinnamon and clove essential oils. Concerning the evaluation of the minimum inhibitory concentration (MIC) for both oils with 0.2, 0.5, and 1% concentrations, B. cereus and B. subtilis were sensitive (+) to cinnamon and clove 0.5%, with inhibition zones of 13.3 and 14 mm for cinnamon and 11.3 and 12 mm for clove EO, respectively. While both bacteria were very sensitive (++) to cinnamon 1% (18.8 and 19.5 mm) and clove 1% (17.3 and 18.7 mm), respectively. Therefore, MRP was prepared by adding cinnamon and clove EOs at 0.6%. Treatments containing EOs showed a significant reduction of tested microorganisms compared to controls. B. cereus wasn’t detected in clove and cinnamon EO treatments at day 21 of the storage period, while B. subtilis vanished on day 14 for the cinnamon treatment and on day 21 for clove MRP. Moreover, the results revealed the enhancement of sensory characteristics of MRP supplemented with EOs without any significant alteration in their pH values. This study recommends the addition of cinnamon and clove EOs (0.6%) to MRP, as it isn’t only an excellent substitution of chemical preservatives with powerful antibacterial efficiency but also improves the overall acceptance of the product.","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":" 18","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141367625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-07DOI: 10.21608/javs.2024.276758.1328
A. Aremu, O. Oridupa, Norazah Bashar
Diabetes mellitus is a major health challenge that has harmful effects on the quality of life globally as a result of its numerous complications. This study aimed to evaluate the positive modulatory effect of Lawsonia inermis (LI) Linn on various haemobiochemical parameters and lipid profiles in streptozotocin-induced diabetic rats. Ten groups of diabetic rats (n = 5) were orally administered 50 and 100 mg/kg of three different fractions of Lawsonia inermis : metformin (500 mg/kg) and glibenclamide (5 mg/kg), whereas untreated hyperglycaemic and normoglycaemic rats received distilled water. The results showed increased red blood cells (RBC) in treated rats compared to untreated diabetic rats. Hb, MCV, MCH, and MCHC decreased nonsignificantly, whereas WBC increased nonsignificantly. Neutrophil increased non-significantly in diabetic, untreated rats, while all the treatment groups decreased non-significantly. Lymphocytes and monocytes increased non-significantly (p > 0.05). Most treatment groups showed a non-significant (p > 0.05) increase in the platelet count. The ALT levels decreased non-significantly (p > 0.05) compared to normoglycemic rats. The AST levels decreased significantly (p<0.01). ALP decreased non-significantly in both treated and untreated groups, whereas bilirubin did not show any significant changes. Creatinine and urea levels in untreated diabetic rats increased non-significantly, while treatment groups decreased non-significantly. Lawsonia inermis -treated rats showed significant improvement in erythrocytic fragility, while glycated haemoglobin in untreated-diabetic and glibenclamide-treated rats increased significantly (p > 0.001). Triglycerides and high-density lipoprotein decreased non-significantly, while low-density lipoprotein increased non-significantly (p > 0.05) in diabetic untreated rats. All treatment groups showed a non-significant (P > 0.05) decrease in low-density lipoprotein. Lawsonia inermis showed a significant positive modulatory effect on the haemobiochemical changes, glycated Hb, osmotic fragility, and lipid profile in streptozotocin-induced diabetic rats.
{"title":"Effect of Different Fractions of Lawsonia inermis Linn on Haematobiochemical Changes, Osmotic Fragility and Lipid Profile in Streptozotocin-induced Diabetic Wistar Rats","authors":"A. Aremu, O. Oridupa, Norazah Bashar","doi":"10.21608/javs.2024.276758.1328","DOIUrl":"https://doi.org/10.21608/javs.2024.276758.1328","url":null,"abstract":"Diabetes mellitus is a major health challenge that has harmful effects on the quality of life globally as a result of its numerous complications. This study aimed to evaluate the positive modulatory effect of Lawsonia inermis (LI) Linn on various haemobiochemical parameters and lipid profiles in streptozotocin-induced diabetic rats. Ten groups of diabetic rats (n = 5) were orally administered 50 and 100 mg/kg of three different fractions of Lawsonia inermis : metformin (500 mg/kg) and glibenclamide (5 mg/kg), whereas untreated hyperglycaemic and normoglycaemic rats received distilled water. The results showed increased red blood cells (RBC) in treated rats compared to untreated diabetic rats. Hb, MCV, MCH, and MCHC decreased nonsignificantly, whereas WBC increased nonsignificantly. Neutrophil increased non-significantly in diabetic, untreated rats, while all the treatment groups decreased non-significantly. Lymphocytes and monocytes increased non-significantly (p > 0.05). Most treatment groups showed a non-significant (p > 0.05) increase in the platelet count. The ALT levels decreased non-significantly (p > 0.05) compared to normoglycemic rats. The AST levels decreased significantly (p<0.01). ALP decreased non-significantly in both treated and untreated groups, whereas bilirubin did not show any significant changes. Creatinine and urea levels in untreated diabetic rats increased non-significantly, while treatment groups decreased non-significantly. Lawsonia inermis -treated rats showed significant improvement in erythrocytic fragility, while glycated haemoglobin in untreated-diabetic and glibenclamide-treated rats increased significantly (p > 0.001). Triglycerides and high-density lipoprotein decreased non-significantly, while low-density lipoprotein increased non-significantly (p > 0.05) in diabetic untreated rats. All treatment groups showed a non-significant (P > 0.05) decrease in low-density lipoprotein. Lawsonia inermis showed a significant positive modulatory effect on the haemobiochemical changes, glycated Hb, osmotic fragility, and lipid profile in streptozotocin-induced diabetic rats.","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":"5 21","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141004383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-07DOI: 10.21608/javs.2024.280576.1329
Ashraf Abu-Seida
.
.
{"title":"Gangrene of the Tongue Develops 48 Hours Following Ovariohysterectomy as a Treatment of Open-Cervix Pyometra in a Bitch","authors":"Ashraf Abu-Seida","doi":"10.21608/javs.2024.280576.1329","DOIUrl":"https://doi.org/10.21608/javs.2024.280576.1329","url":null,"abstract":".","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":"23 37","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141004792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-05-06DOI: 10.21608/javs.2024.276537.1324
H. Rahma, Dh. Jwher
The present study aimed to isolate and identify Methicillin-Resistant Staphylococcus aureus MRSA from the nasal cavity of healthy cows and buffaloes and their breeders in Nineveh Governorate and detect some virulence factors by using molecular methods. A total of 150 samples of cotton swabs were collected randomly from different areas of Nineveh governorate. The samples were cotton swabs from the nasal passages of healthy cattle, buffaloes, and their breeders (50 swabs of each type). All the samples were subjected to culture and molecular testing. The results showed the highest isolation percentage of S. aureus from cattle followed by breeders, then buffaloes, at 54%, 40%, and 32%, respectively. The total isolation percentage of MRSA was 65.1%. The highest percentage was in buffaloes, followed by breeders and cattle, at 93.75%, 70%, and 44.44%, respectively. Out of 41 isolates from cattle, buffaloes, and their breeders, the virulence genes clf A, clf B, and coa were detected in MRSA at rates of 100%, 80.49%, and 65.85%, respectively. The current study concluded that cattle and buffalo are considered carriers and potential transmitters of MRSA, which makes them risk factors for infection in humans, especially those who are in direct contact with animals. Together, these findings also highlight the need to prevent the transmission of zoonotic pathogens to humans via occupational exposure or consumption of contaminated animal products.
{"title":"Detection of clfA, clfB and coa genes in Methicillin-Resistant Staphylococcus aureus (MRSA) isolated from Nasal Cavity of Cows, Buffalo and their Breeders in Nineveh Governorate, Iaq","authors":"H. Rahma, Dh. Jwher","doi":"10.21608/javs.2024.276537.1324","DOIUrl":"https://doi.org/10.21608/javs.2024.276537.1324","url":null,"abstract":"The present study aimed to isolate and identify Methicillin-Resistant Staphylococcus aureus MRSA from the nasal cavity of healthy cows and buffaloes and their breeders in Nineveh Governorate and detect some virulence factors by using molecular methods. A total of 150 samples of cotton swabs were collected randomly from different areas of Nineveh governorate. The samples were cotton swabs from the nasal passages of healthy cattle, buffaloes, and their breeders (50 swabs of each type). All the samples were subjected to culture and molecular testing. The results showed the highest isolation percentage of S. aureus from cattle followed by breeders, then buffaloes, at 54%, 40%, and 32%, respectively. The total isolation percentage of MRSA was 65.1%. The highest percentage was in buffaloes, followed by breeders and cattle, at 93.75%, 70%, and 44.44%, respectively. Out of 41 isolates from cattle, buffaloes, and their breeders, the virulence genes clf A, clf B, and coa were detected in MRSA at rates of 100%, 80.49%, and 65.85%, respectively. The current study concluded that cattle and buffalo are considered carriers and potential transmitters of MRSA, which makes them risk factors for infection in humans, especially those who are in direct contact with animals. Together, these findings also highlight the need to prevent the transmission of zoonotic pathogens to humans via occupational exposure or consumption of contaminated animal products.","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":"5 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141006389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-17DOI: 10.21608/javs.2024.266563.1311
Masao Arisato, Shozo Okano
To elucidate the relationship between obesity, awakening time, and anesthesia in dogs, we conducted a comparative analysis of the awakening time following isoflurane and sevoflurane anesthesia in both standard-weight and obese groups. Six female beagle dogs within the standard weight range underwent 1-hour inhalation anesthesia with either isoflurane or sevoflurane. We recorded the time of extubation, head lift, and standing. Additionally, a parallel experiment was conducted on the same test animals after inducing obesity through nutritional management, with criteria for obesity defined as a body conditioning score of 4 or higher and a body fat percentage of 30% or higher. Under isoflurane anesthesia, we observed a tendency for a longer awakening time in the obese group compared to the standard weight group. However, this trend was not evident with sevoflurane anesthesia. Furthermore, when comparing awakening time with both anesthetics, no significant difference was found between the standard weight groups. In contrast, within the obese group, awakening time was significantly prolonged with isoflurane anesthesia compared to sevoflurane anesthesia. These results confirm that obesity contributes to delay awakening in dogs. Additionally, in the anesthesia management of obese dogs, the use of sevoflurane is anticipated to reduce awakening time compared to isoflurane.
{"title":"Effect of Obesity on Awakening Time after Isoflurane/Sevoflurane Inhalation Anesthesia in Dogs","authors":"Masao Arisato, Shozo Okano","doi":"10.21608/javs.2024.266563.1311","DOIUrl":"https://doi.org/10.21608/javs.2024.266563.1311","url":null,"abstract":"To elucidate the relationship between obesity, awakening time, and anesthesia in dogs, we conducted a comparative analysis of the awakening time following isoflurane and sevoflurane anesthesia in both standard-weight and obese groups. Six female beagle dogs within the standard weight range underwent 1-hour inhalation anesthesia with either isoflurane or sevoflurane. We recorded the time of extubation, head lift, and standing. Additionally, a parallel experiment was conducted on the same test animals after inducing obesity through nutritional management, with criteria for obesity defined as a body conditioning score of 4 or higher and a body fat percentage of 30% or higher. Under isoflurane anesthesia, we observed a tendency for a longer awakening time in the obese group compared to the standard weight group. However, this trend was not evident with sevoflurane anesthesia. Furthermore, when comparing awakening time with both anesthetics, no significant difference was found between the standard weight groups. In contrast, within the obese group, awakening time was significantly prolonged with isoflurane anesthesia compared to sevoflurane anesthesia. These results confirm that obesity contributes to delay awakening in dogs. Additionally, in the anesthesia management of obese dogs, the use of sevoflurane is anticipated to reduce awakening time compared to isoflurane.","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":" 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140390823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-10DOI: 10.21608/javs.2024.266431.1312
A. Atiyah, Alkattan L.M.
Histopathological and immunohistochemical assessment of fabricated coral shell hydroxyapatite (CSHA) and plasma rich fibrin (PRF) in remodeling of the induced critical size defect of the mandibular bone in the dogs: Twenty-seven adult dogs of both sexes were included and equally divided into three equal groups: control, plasma-rich fibrin (PRF) and hydroxyapatite group (CSHA). The experimental mandibular bone defect was induced in a circular shape, and the dimensions of the defect were 14×5mm. Evaluation of the healing progress of the defect and associated macroscopical, histopathological, and Immunohistological findings was recorded in all studied groups at 7, 15, and 30 days post-operatively. Macroscopically, the healing was evaluated by the presence of new bone tissue filling the bone gap defect in all groups during different follow-up periods. In the plasma-rich fibrin (PRF) group, the gap was highly filled with hard, firm tissues that filled all borders and the centre of the induced gap in comparison with the coral shell hydroxyl apatite group (CSHA), which is partially filled with hard tissue. Histopathologically, the progress of healing in the PRF group was represented by the presence of highly mature connective tissue and new woven bone formation at seven days and well-developed mature bone inside defective bone at 15 and 30 days post-operatively, whereas in the CSHA group, the results were represented by the occlusion of highly mature connective tissue and new woven bone formation inside the induced hole at 15 and 30 days post-operatively. At 30 days post-surgery, in the control group, there was the presence of newly formed woven bone surrounded by the edge of the mandible bone. The immunohistochemical expression of the alkaline phosphatase (ALP) in the mandible bone at 30 days PS in the control group was represented by weak positive expression, while mild positive expression was indicated in the CSHA group and moderate positive expression in the PRF group. In conclusion, this research exhibited the role of both CSHA and PRF in improving the healing process of defective mandible bones, with a clear superiority of the beneficial value of using PRF. The histopathological and immunohistochemistry assessments emphasize these results.
{"title":"Impact of Fabricated Coral Shell Hydroxyapatite Powder and Autologous Plasma Rich- fibrin in Remodeling of the Mandibular Bone Critical Size Defect in Dogs: Histopathological and Immunohistochemical Study","authors":"A. Atiyah, Alkattan L.M.","doi":"10.21608/javs.2024.266431.1312","DOIUrl":"https://doi.org/10.21608/javs.2024.266431.1312","url":null,"abstract":"Histopathological and immunohistochemical assessment of fabricated coral shell hydroxyapatite (CSHA) and plasma rich fibrin (PRF) in remodeling of the induced critical size defect of the mandibular bone in the dogs: Twenty-seven adult dogs of both sexes were included and equally divided into three equal groups: control, plasma-rich fibrin (PRF) and hydroxyapatite group (CSHA). The experimental mandibular bone defect was induced in a circular shape, and the dimensions of the defect were 14×5mm. Evaluation of the healing progress of the defect and associated macroscopical, histopathological, and Immunohistological findings was recorded in all studied groups at 7, 15, and 30 days post-operatively. Macroscopically, the healing was evaluated by the presence of new bone tissue filling the bone gap defect in all groups during different follow-up periods. In the plasma-rich fibrin (PRF) group, the gap was highly filled with hard, firm tissues that filled all borders and the centre of the induced gap in comparison with the coral shell hydroxyl apatite group (CSHA), which is partially filled with hard tissue. Histopathologically, the progress of healing in the PRF group was represented by the presence of highly mature connective tissue and new woven bone formation at seven days and well-developed mature bone inside defective bone at 15 and 30 days post-operatively, whereas in the CSHA group, the results were represented by the occlusion of highly mature connective tissue and new woven bone formation inside the induced hole at 15 and 30 days post-operatively. At 30 days post-surgery, in the control group, there was the presence of newly formed woven bone surrounded by the edge of the mandible bone. The immunohistochemical expression of the alkaline phosphatase (ALP) in the mandible bone at 30 days PS in the control group was represented by weak positive expression, while mild positive expression was indicated in the CSHA group and moderate positive expression in the PRF group. In conclusion, this research exhibited the role of both CSHA and PRF in improving the healing process of defective mandible bones, with a clear superiority of the beneficial value of using PRF. The histopathological and immunohistochemistry assessments emphasize these results.","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":"23 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140396598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-07DOI: 10.21608/javs.2024.262746.1309
Mohammed Adam, A. Ajadi, J. Atata, Olatunde David Akanbi, S. Shoyinka, Muhammad Abubakar, Moshood Olaniyi
Canine pathologies refer to the various diseases, disorders, and abnormalities that can affect a dog’s different body systems and cause mortality. The primary aim of this study was to investigate the common pathologies that are associated with the mortality of dogs in Ilorin, Kwara State. A retrospective review of post-mortem records from 7 years was undertaken at a necropsy unit of the Department of Veterinary Pathology, University of Ilorin. Data collected included age, sex, breed, disease pathogens, and year. A total of 150 dog carcasses were presented for necropsy during the period between January 2016 and December 2022. In this study, the cases used were confirmed based on PCR results in some cases, bacterial culture and isolation, parasite identification, gross lesions, and histopathological findings in other cases. Descriptive statistics were employed to evaluate the effect of disease pathogens on age, sex, and breed distribution patterns associated with the mortality of dogs. The prevalence of viral infection among other diseases was statistically significant (34.00%; OR = 0.53; P<0.01). The percentage prevalence of each disease showed that bacterial infections were evident, with Staphylococcosis being the most prevalent at 49.01%, followed by Salmonellosis at 7.84%, Leptospirosis at 23.53%, and Brucellosis at 19.6%. Parasitic infections were also notable, encompassing canine babesiosis (34.80%), acariosis (39.10%), and canine ehrlichiosis (26.08%). Toxicosis cases were largely dominated by diclovous poisoning, constituting 88.88% of instances, while medication toxicity was observed in 11.11% of cases. Neoplastic conditions were represented by hepatic lymphoma, with a prevalence of 1%, while nutritional deficiencies included hepatic lipidosis, noted in 1% of cases. Traumatic injuries were common, with bone fracture having the highest prevalence at 66.67% and muscle laceration observed in 33.33% of cases. Intestinal obstruction cases, specifically gastric impaction, were noted in 1% of cases. Viral infections comprised parvovirus infection with the highest prevalence at 81%, and canine distemper was observed in 18.92% of cases. In conclusion, this study has exposed the leading causes of mortalities among the dog population, which include parvovirus enteritis, staphylococcosis, and acariosis infections. The emphasis should be on preventive measures to curtail disease transmission and associated financial loss.
{"title":"Retrospective Study of Canine Pathologies Causing Mortalities in Ilorin Kwara State, Nigeria","authors":"Mohammed Adam, A. Ajadi, J. Atata, Olatunde David Akanbi, S. Shoyinka, Muhammad Abubakar, Moshood Olaniyi","doi":"10.21608/javs.2024.262746.1309","DOIUrl":"https://doi.org/10.21608/javs.2024.262746.1309","url":null,"abstract":"Canine pathologies refer to the various diseases, disorders, and abnormalities that can affect a dog’s different body systems and cause mortality. The primary aim of this study was to investigate the common pathologies that are associated with the mortality of dogs in Ilorin, Kwara State. A retrospective review of post-mortem records from 7 years was undertaken at a necropsy unit of the Department of Veterinary Pathology, University of Ilorin. Data collected included age, sex, breed, disease pathogens, and year. A total of 150 dog carcasses were presented for necropsy during the period between January 2016 and December 2022. In this study, the cases used were confirmed based on PCR results in some cases, bacterial culture and isolation, parasite identification, gross lesions, and histopathological findings in other cases. Descriptive statistics were employed to evaluate the effect of disease pathogens on age, sex, and breed distribution patterns associated with the mortality of dogs. The prevalence of viral infection among other diseases was statistically significant (34.00%; OR = 0.53; P<0.01). The percentage prevalence of each disease showed that bacterial infections were evident, with Staphylococcosis being the most prevalent at 49.01%, followed by Salmonellosis at 7.84%, Leptospirosis at 23.53%, and Brucellosis at 19.6%. Parasitic infections were also notable, encompassing canine babesiosis (34.80%), acariosis (39.10%), and canine ehrlichiosis (26.08%). Toxicosis cases were largely dominated by diclovous poisoning, constituting 88.88% of instances, while medication toxicity was observed in 11.11% of cases. Neoplastic conditions were represented by hepatic lymphoma, with a prevalence of 1%, while nutritional deficiencies included hepatic lipidosis, noted in 1% of cases. Traumatic injuries were common, with bone fracture having the highest prevalence at 66.67% and muscle laceration observed in 33.33% of cases. Intestinal obstruction cases, specifically gastric impaction, were noted in 1% of cases. Viral infections comprised parvovirus infection with the highest prevalence at 81%, and canine distemper was observed in 18.92% of cases. In conclusion, this study has exposed the leading causes of mortalities among the dog population, which include parvovirus enteritis, staphylococcosis, and acariosis infections. The emphasis should be on preventive measures to curtail disease transmission and associated financial loss.","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":"18 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140260036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-07DOI: 10.21608/javs.2024.266445.1310
Taimaa Yahya, Yaareb Mousa
Nowadays, there is a need for good and efficacious NSAIDs with minimal side effects to be applied in veterinary medicine. The aim was to compare the pharmacodynamics (analgesia and inhibition of COX-2) and pharmacokinetics between selective (nimesulide) and non-selective (aspirin) COX-2 inhibitors in mice. Assessing the median effective doses by using the up-and-down method, COX-2 activity and plasma concentrations for both nimesulide and aspirin with their pharmacokinetic profiles in mice. The median effective doses (ED50s) of nimesulide and aspirin were found to be 7.9 and 212.23 mg/kg, respectively, using the hot-plate. Both nimesulide (15.8 mg/kg, i.m.) and aspirin (424.5 mg/kg, i.m.) inhibited COX-2 activity through a decrease in COX-2 concentrations in the plasma, liver, and kidney of mice, with superior inhibition when administering nimesulide in comparison to the control (negative and positive) and aspirin-treated groups. Plasma concentrations of nimesulide (15.8 mg/kg, i.m.) measured for different comparable periods of 0.5, 1, 2, 4, and 24 hours were higher than those of aspirin, which were 14.62, 9.22, 9.88, 7.38 and 2.27 µg/ml, respectively, while aspirin (424.5 mg/kg, i.m.) was 4.35, 3.17, 2.54, 2.25 and 1.21 µg/ml, at the same measured times. Nimesulide pharmacokinetic variables were estimated to be AUC0-∞ 169.18, AUMC0-∞ 2358,72, Kel 0.06, Cmax 14.62, Tmax 0.5, t1/2β 11.07, MRT 13.94, Vss 1.49, and Cl 0.09, while aspirin pharmacokinetic parameters differed to be 82.31, 2428.32, 0.03, 4.35, 0.5, 21.25, 158.12, and 5.16, respectively. The study concluded that nimesulide has superior pharmacological properties (analgesic, antipyretic, and anti-inflammatory) than aspirin due to its ability to inhibit COX-2 more selectively and its unique pharmacokinetics in mice, which may be useful in veterinary medicine.
{"title":"Pharmacodynamic and Pharmacokinetic Comparison between Selective and Non-selective COX-2 Inhibitors in Mice","authors":"Taimaa Yahya, Yaareb Mousa","doi":"10.21608/javs.2024.266445.1310","DOIUrl":"https://doi.org/10.21608/javs.2024.266445.1310","url":null,"abstract":"Nowadays, there is a need for good and efficacious NSAIDs with minimal side effects to be applied in veterinary medicine. The aim was to compare the pharmacodynamics (analgesia and inhibition of COX-2) and pharmacokinetics between selective (nimesulide) and non-selective (aspirin) COX-2 inhibitors in mice. Assessing the median effective doses by using the up-and-down method, COX-2 activity and plasma concentrations for both nimesulide and aspirin with their pharmacokinetic profiles in mice. The median effective doses (ED50s) of nimesulide and aspirin were found to be 7.9 and 212.23 mg/kg, respectively, using the hot-plate. Both nimesulide (15.8 mg/kg, i.m.) and aspirin (424.5 mg/kg, i.m.) inhibited COX-2 activity through a decrease in COX-2 concentrations in the plasma, liver, and kidney of mice, with superior inhibition when administering nimesulide in comparison to the control (negative and positive) and aspirin-treated groups. Plasma concentrations of nimesulide (15.8 mg/kg, i.m.) measured for different comparable periods of 0.5, 1, 2, 4, and 24 hours were higher than those of aspirin, which were 14.62, 9.22, 9.88, 7.38 and 2.27 µg/ml, respectively, while aspirin (424.5 mg/kg, i.m.) was 4.35, 3.17, 2.54, 2.25 and 1.21 µg/ml, at the same measured times. Nimesulide pharmacokinetic variables were estimated to be AUC0-∞ 169.18, AUMC0-∞ 2358,72, Kel 0.06, Cmax 14.62, Tmax 0.5, t1/2β 11.07, MRT 13.94, Vss 1.49, and Cl 0.09, while aspirin pharmacokinetic parameters differed to be 82.31, 2428.32, 0.03, 4.35, 0.5, 21.25, 158.12, and 5.16, respectively. The study concluded that nimesulide has superior pharmacological properties (analgesic, antipyretic, and anti-inflammatory) than aspirin due to its ability to inhibit COX-2 more selectively and its unique pharmacokinetics in mice, which may be useful in veterinary medicine.","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":"52 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140258835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-06DOI: 10.21608/javs.2024.262263.1307
Ahmad Salisu, Abubakar Adamu Abdul, Buhari Salisu, A. Salisu, Yakubu Abubakar Sadiq, Peter Charles
The stainless-steel orthopaedic implants used for the management of various types of fracture internal fixations are very expensive; this makes their utilization in food animals relatively noneconomical. There is a need to have an improvised implant that is cheaper and available for use in food animals to manage complicated fractures requiring open reduction and internal fixation. The objective of this study is to evaluate the haematological and biochemical changes following the reduction of stable femoral fractures in goats using conventional (Kirschner pin) and improvised chrome vanadium-coated long crews. Twelve apparently healthy Red Sokoto bucks were randomly divided into two groups of six (n=6) were used for this study. A transverse femoral diaphyseal fracture was created using orthopaedic wire in both groups. In group A, a conventional Kirschner pin size 4.0x125 mm 2 , single trocar, non-threaded, was used to immobilize the fracture, while in group B, an improvised test chrome vanadium (long screw) size 2.3 mm was used for the fracture immobilizations. Blood samples were collected before fracture induction at the base line, serving as a control. The haematological and biochemical assessments were performed at 0 weeks (immediate postoperative) and subsequently at 2, 4, 6, 8, 10 and 12 weeks postoperatively. Packed cell volume (PCV%), haemoglobin concentration (Hb), red blood cell count (RBCs), white blood cell count (WBCs) and differential leukocytic count (neutrophils, lymphocytes and monocytes) were evaluated. Also, serum activity of alkaline phosphatase (ALP), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) was detected. Besides, serum calcium, phosphorous and creatinine levels were evaluated. There were no significant differences between the two groups in PCV, haemoglobin, RBCs, WBCs and neutrophils, and the values were within the normal range. However, lymphocytes and monocytes were significantly (p<0.05) different at weeks 2 and 6, respectively. Biochemical parameters revealed significant (p<0.05) changes in serum ALT (weeks 0, 2, 4), ALP (week 6), creatinine (weeks 10 and 12), and calcium (week 2) at some postoperative intervals. However, no variations were observed in serum AST and phosphorous, which were within the normal range. The improvised chrome vanadium (Long screw) can be used safely in goats for the management of stable femoral fractures without significant adverse changes to hemato-biochemical profiles within twelve weeks.
{"title":"Haemato-biochemical Response to Kirschner Pin and Improvised Chrome Vanadium Long Screw Used for the Stabilization of Femoral Fracture in Goats","authors":"Ahmad Salisu, Abubakar Adamu Abdul, Buhari Salisu, A. Salisu, Yakubu Abubakar Sadiq, Peter Charles","doi":"10.21608/javs.2024.262263.1307","DOIUrl":"https://doi.org/10.21608/javs.2024.262263.1307","url":null,"abstract":"The stainless-steel orthopaedic implants used for the management of various types of fracture internal fixations are very expensive; this makes their utilization in food animals relatively noneconomical. There is a need to have an improvised implant that is cheaper and available for use in food animals to manage complicated fractures requiring open reduction and internal fixation. The objective of this study is to evaluate the haematological and biochemical changes following the reduction of stable femoral fractures in goats using conventional (Kirschner pin) and improvised chrome vanadium-coated long crews. Twelve apparently healthy Red Sokoto bucks were randomly divided into two groups of six (n=6) were used for this study. A transverse femoral diaphyseal fracture was created using orthopaedic wire in both groups. In group A, a conventional Kirschner pin size 4.0x125 mm 2 , single trocar, non-threaded, was used to immobilize the fracture, while in group B, an improvised test chrome vanadium (long screw) size 2.3 mm was used for the fracture immobilizations. Blood samples were collected before fracture induction at the base line, serving as a control. The haematological and biochemical assessments were performed at 0 weeks (immediate postoperative) and subsequently at 2, 4, 6, 8, 10 and 12 weeks postoperatively. Packed cell volume (PCV%), haemoglobin concentration (Hb), red blood cell count (RBCs), white blood cell count (WBCs) and differential leukocytic count (neutrophils, lymphocytes and monocytes) were evaluated. Also, serum activity of alkaline phosphatase (ALP), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) was detected. Besides, serum calcium, phosphorous and creatinine levels were evaluated. There were no significant differences between the two groups in PCV, haemoglobin, RBCs, WBCs and neutrophils, and the values were within the normal range. However, lymphocytes and monocytes were significantly (p<0.05) different at weeks 2 and 6, respectively. Biochemical parameters revealed significant (p<0.05) changes in serum ALT (weeks 0, 2, 4), ALP (week 6), creatinine (weeks 10 and 12), and calcium (week 2) at some postoperative intervals. However, no variations were observed in serum AST and phosphorous, which were within the normal range. The improvised chrome vanadium (Long screw) can be used safely in goats for the management of stable femoral fractures without significant adverse changes to hemato-biochemical profiles within twelve weeks.","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":"11 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140262164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-24DOI: 10.21608/javs.2024.262508.1308
A. E. Eissa
Mass fish kills are abrupt events in which a significant number of fish of different ages and species perish in a specific aquatic region. Dramatic waves of mass kills have involved a large variety of economic fish species in both Egyptian fisheries and the aquaculture sector. Several episodes of mass mortalities of various infectious and noninfectious aetiologies have targeted a wide spectrum of freshwater and marine fish in both open-water and captive environments. Kafr El-Sheikh, Sharkia, Dakahlia, Fayoum, Alexandria and Giza were the most affected Egyptian provinces. The Mediterranean basin, Lake Manzala, Lake Mariott, Lake Burullus, River Nile, and some provincial water streams and municipal water drains were all reported to have had one or more episodes of mass kills over the past two decades. These mass kills were mainly attributed to different types / magnitudes of aquatic environmental pollution, which was explicitly aggravated by poor aquaculture / fishing practices. Further, the frequent absence of veterinary guidance has had a great negative impact on providing possible solutions for this disaster in the near future. Ultimately, adopting Good Aquaculture Practice (GAP), competent biosecurity strategies, and regular veterinary supervision will be the most practical solutions that ensure the reliable growth and sustainability of both Egyptian fisheries and aquaculture sectors.
鱼类大量死亡是指在一个特定的水域中,大量不同年龄和种类的鱼类死亡的突发事件。在埃及渔业和水产养殖业中,各种经济鱼类都曾出现过大规模死亡的现象。在开放水域和人工饲养环境中,各种感染性和非感染性病原体导致的大规模死亡事件已针对多种淡水鱼和海水鱼。Kafr El-Sheikh、Sharkia、Dakahlia、Fayoum、Alexandria 和 Giza 是埃及受影响最严重的省份。据报告,地中海盆地、曼扎拉湖、马里奥特湖、布鲁鲁斯湖、尼罗河以及一些省份的溪流和市政排水沟在过去二十年里都发生过一次或多次大规模死亡事件。这些大规模死亡事件主要归因于不同类型/程度的水生环境污染,而不良的水产养殖/捕鱼方式则明显加剧了污染。此外,兽医指导的频繁缺失也对在不久的将来为这场灾难提供可能的解决方案产生了极大的负面影响。最终,采用良好水产养殖规范 (GAP)、有效的生物安全策略和定期的兽医监督将是确保埃及渔业和水产养殖业可靠增长和可持续发展的最切实可行的解决方案。
{"title":"A mini-review on fish mass kills within the Egyptian fisheries and aquaculture sectors: Impacts and proposed solutions","authors":"A. E. Eissa","doi":"10.21608/javs.2024.262508.1308","DOIUrl":"https://doi.org/10.21608/javs.2024.262508.1308","url":null,"abstract":"Mass fish kills are abrupt events in which a significant number of fish of different ages and species perish in a specific aquatic region. Dramatic waves of mass kills have involved a large variety of economic fish species in both Egyptian fisheries and the aquaculture sector. Several episodes of mass mortalities of various infectious and noninfectious aetiologies have targeted a wide spectrum of freshwater and marine fish in both open-water and captive environments. Kafr El-Sheikh, Sharkia, Dakahlia, Fayoum, Alexandria and Giza were the most affected Egyptian provinces. The Mediterranean basin, Lake Manzala, Lake Mariott, Lake Burullus, River Nile, and some provincial water streams and municipal water drains were all reported to have had one or more episodes of mass kills over the past two decades. These mass kills were mainly attributed to different types / magnitudes of aquatic environmental pollution, which was explicitly aggravated by poor aquaculture / fishing practices. Further, the frequent absence of veterinary guidance has had a great negative impact on providing possible solutions for this disaster in the near future. Ultimately, adopting Good Aquaculture Practice (GAP), competent biosecurity strategies, and regular veterinary supervision will be the most practical solutions that ensure the reliable growth and sustainability of both Egyptian fisheries and aquaculture sectors.","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":"41 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140434305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}