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Isolation and Antibiogram Profiles of Staphylococcus aureus Isolates from Cow milk and Dog samples 牛奶和狗样品中金黄色葡萄球菌分离株的分离及其抗体谱
Pub Date : 2022-11-15 DOI: 10.21608/javs.2022.164610.1181
A. Zedan, N. Alatfeehy, S. Marouf
Staphylococcus aureus ( S. aureus ) is a commensal bacterium associated with serious infections in men and animals. Recently, multidrug-resistant (MDR) strains of S. aureus especially the so-called methicillin-resistant S. aureus (MRSA), represent a serious challenge that hinders the control of infections in man and animals. This study aimed to monitor milk samples from mastitic cows and vaginal and nasal swabs from dogs for the incidence of S. aureus . In addition, the isolates' antibiogram profiles were assessed to determine the extent of MDR and MRSA existence among the recovered isolates. Out of 260 samples, 29 (11%) S. aureus isolates were recovered with the highest incidence in milk samples (15/90, 17%), followed by vaginal swabs (8/90, 9%) and nasal swabs (6/80, 7%). Identification of the isolates was confirmed by PCR amplification of 16S rRNA gene sequence. Twenty S. aureus isolates were tested against seven antibacterial agents. Surprisingly, all the twenty isolates were MRSA and three bitch vaginal isolates were MDR. The findings of this study call for more research and cooperation between authors interested in assessing the MRSA and MRD bacterial incidence in both medical and veterinary fields. The cooperation will augment the challenge of disseminating MRSA and MDR staphylococci from animals to humans and vice versa.
金黄色葡萄球菌(金黄色葡萄球菌)是一种与人类和动物严重感染相关的共生细菌。最近,耐多药金黄色葡萄球菌(MDR)菌株,特别是所谓的耐甲氧西林金黄色葡萄球菌(MRSA),对人类和动物感染的控制构成了严重的挑战。这项研究的目的是监测乳腺炎奶牛的牛奶样本和狗的阴道和鼻腔拭子的金黄色葡萄球菌的发病率。此外,对分离株的抗生素谱进行了评估,以确定在回收的分离株中存在MDR和MRSA的程度。260份样本中检出29株(11%)金黄色葡萄球菌,其中乳样感染率最高(15/90,17%),其次为阴道拭子(8/90,9%)和鼻拭子(6/80,7%)。通过16S rRNA基因序列的PCR扩增证实了分离株的身份。对20株金黄色葡萄球菌进行了抗7种抗菌药物的试验。令人惊讶的是,20株分离株均为MRSA, 3株母狗阴道分离株为MDR。这项研究的结果呼吁对MRSA和MRD细菌在医学和兽医领域的发病率评估感兴趣的作者进行更多的研究和合作。这一合作将增加MRSA和MDR葡萄球菌从动物向人类传播的挑战,反之亦然。
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引用次数: 1
Preparation of a Combined Inactivated Vaccine against Riemerella anatipestifer and Duck Viral Hepatitis 鸭疫里默氏菌和鸭病毒性肝炎联合灭活疫苗的研制
Pub Date : 2022-11-15 DOI: 10.21608/javs.2022.163930.1179
S. Abodalal, M. Ismail, Eman El Rawy
Riemerella anatipestifer ( R. anatipestifer ) infection and duck virus hepatitis (DVH) are enormous dangers for the duck industry and its investment. So, in the current study, a combined inactivated vaccine against both of them was prepared to combat their adverse effect. One hundred and thirty-three ducks of one-day-old of age were used and grouped randomly into four groups. Group (1) was vaccinated with R. anatipestifer vaccine, group (2) was vaccinated with DHV vaccine, group (3) was vaccinated with the prepared combined vaccine of both and finally, group (4) was kept as a negative control. Vaccination was at one day old of age. The vaccinated groups with Riemerella vaccine had 72.7% protection against challenges with the virulent strain with the highest antibody titers in 6 th week as measured by the indirect Hemagglutination test. The control group had 90.9% mortality when challenged against R. anatipestifer , with no detectable antibody titers. DVH-vaccinated groups exhibited their highest serum-neutralizing antibody titers by the 5 th and 6 th weeks post-vaccination. The Control group had no detectable antibody titers against DVH. Statistically, it was clear that there were no significant differences between the results of different groups vaccinated with combined or single vaccines of the same agent. Briefly, combined vaccines of R. Anatipestifer and duck viral hepatitis have harmonized effects with the priority to decrease the stress on birds and workers. Besides its efficiency, the economic side as providing one-shot vaccines instead of each one separately.
鸭疫里默氏菌(R. anatipestier)感染和鸭病毒性肝炎(DVH)是鸭业及其投资的巨大威胁。因此,在目前的研究中,准备了一种针对这两种病毒的联合灭活疫苗来对抗它们的副作用。选用1日龄肉鸭133只,随机分为4组。接种鸭疫R.疫苗组(1),接种DHV疫苗组(2),接种配制的两种疫苗联合疫苗组(3),最后将组(4)作为阴性对照。接种疫苗是在一天大的时候。间接血凝试验结果显示,接种里默氏疫苗的组在第6周对抗体滴度最高的毒力菌株的攻击有72.7%的保护作用。未检测到抗体滴度的对照组鸭疫鼠攻毒死亡率为90.9%。接种dvh疫苗组在接种后第5周和第6周血清中和抗体滴度最高。对照组未检测到DVH抗体滴度。从统计学上看,接种同一种疫苗的联合疫苗或单一疫苗的不同组之间的结果显然没有显著差异。简而言之,鸭疫R.和鸭病毒性肝炎联合疫苗的效果协调一致,重点是减少对鸟类和工人的压力。除了效率之外,经济方面是提供一次性疫苗而不是每一种疫苗。
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引用次数: 0
Detergent Maceration: A Convenient Skeleton Preparation Technique for Teaching and Demonstration of Veterinary Anatomy 洗洁精浸渍:兽医解剖教学演示中一种方便的骨架制备技术
Pub Date : 2022-11-15 DOI: 10.21608/javs.2022.158955.1175
Md. Ashraf Zaman Faruk, S. Das
The current study investigates to develop a convenient technique for preparation of a dog skeleton without any unpleasant smell, cracking, crumbling or discoloration of bones by using a combination of commercially available detergent and Hydrogen peroxide (H 2 O 2 ). Specimens were skinned and immersed in a pre-heated 10-liter detergent solution at 50°C to 60°C for 2-3 hours then left for 12 hours. Maceration was deemed completed when any remaining soft tissue was easily removed under running tap water or by soft brush. Then the bones were kept for bleaching with 3% H 2 O 2 for 24-48 hours and allowed to dry properly in the sun. It is shown that the detergent maceration was remarkably faster compared to the traditional burial, insect, hot or cool-water methods, which requires up to several days. In this entire process, skeletons were prepared and installed in correct conformation in only about 92 hours (less than four days). In contrast, the whole maceration was completed in 15 hours (boiling and cooling). On the other hand, the longest time was taken for bleaching and drying purposes, which were 2 days, respectively. The method investigated here for the preparation of skeletal materials in an essentially odorless way, which has shown to be more convenient (faster, easier, safer, and cheaper) compare to previous models in the fields of veterinary anatomy. We believe this project's result will help improve the preparation and preservation technique of skeletons for gross anatomical studies in veterinary institutions.
目前的研究旨在开发一种方便的技术,通过使用商用洗涤剂和过氧化氢(H2 O2)的组合来制备狗骨架,而不会产生任何难闻的气味、开裂、碎裂或变色。将样品去皮,并在50°C至60°C的预热10升洗涤剂溶液中浸泡2-3小时,然后放置12小时。当任何残留的软组织在自来水下或用软刷轻易去除时,就认为完成了Macation。然后将骨头用3%H2 O2漂白24-48小时,并在阳光下适当干燥。研究表明,与传统的埋葬、昆虫、热水或冷水浸泡方法相比,洗涤剂浸泡速度明显更快,后者需要几天的时间。在整个过程中,仅用了大约92小时(不到4天),骨架就以正确的构象制备并安装好了。相反,整个浸渍在15小时内完成(沸腾和冷却)。另一方面,漂白和干燥的时间最长,分别为2天。本文研究的骨骼材料制备方法基本上没有气味,与兽医解剖学领域的以前模型相比,这种方法更方便(更快、更容易、更安全、更便宜)。我们相信,该项目的成果将有助于改进兽医机构骨骼的制备和保存技术,以进行大体解剖研究。
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引用次数: 0
Polycyclic Aromatic Hydrocarbons in Grilled Meats from Restaurants 餐馆烤肉中的多环芳烃
Pub Date : 2022-11-15 DOI: 10.21608/javs.2022.158847.1174
Abdel-Azeem Abdel-Latif, Fatma H. M. A., Jehan M. M. Ouf, Mohamed H. H. Roby, N. Abdel-Atty
Polycyclic aromatic hydrocarbons are a group of lipophilic compounds that can be generated during the preparation of food items at elevated temperatures. They are regarded as potentially genotoxic and carcinogenic to human beings, related to increased incidence of breast and colorectal cancers, oxidative DNA injury, and bad effects on children neuro-differentiation. Thus, they are considered a public health concerns. A total of thirty samples of grilled beef steak, beef kofta and chicken (ten each) were collected from different restaurants. The samples were extracted by magnesium sulfate and sodium acetate in acetonitrile then purified in magnesium sulfate, primary, secondary amine and silica gel, and finally measured by gas chromatography-mass spectrometry (GC-MS). Benzo[a]pyrene was recorded with the highest average level (3.63µg/kg) in grilled kofta samples, but it was not detected in chicken samples. On the other hand, PAH4, PAH8 and ƩPAHs content were more abundant in grilled beef steak (5.32, 9.97 and 56.91µg/kg). Meanwhile, they recorded the least concentrations of grilled chicken from different restaurants. Furthermore, benzo[a]pyrene exceeded the permissible limits of the European Commission and Egyptian National Food Safety Authority in grilled kofta samples; further studies are needed to investigate the limits of exposure to these harmful compounds from meats and other food items. ــــــــــــــــــــــ
多环芳烃是一组亲脂化合物,在高温下制备食品时可以产生。它们被认为对人类具有潜在的遗传毒性和致癌性,与乳腺癌和结直肠癌的发病率增加、DNA氧化损伤以及对儿童神经分化的不良影响有关。因此,它们被认为是一个公共卫生问题。从不同的餐厅共收集了30份烤牛排、炸牛肉和鸡肉样品(各10份)。样品用硫酸镁和乙酸钠在乙腈中提取,然后用硫酸镁、伯胺、仲胺和硅胶纯化,最后用气相色谱-质谱(GC-MS)测定。在烤肉丸样品中,苯并[a]芘的平均含量最高(3.63µg/kg),但在鸡肉样品中未检测到。烤牛排中PAH4、PAH8和ƩPAHs含量较高(分别为5.32、9.97和56.91µg/kg)。同时,他们记录了来自不同餐馆的烤鸡的最低浓度。此外,烤肉丸样品中的苯并[a]芘超过了欧洲委员会和埃及国家食品安全局的允许限量;需要进一步的研究来调查从肉类和其他食物中接触这些有害化合物的限度。ــــــــــــــــــــــ
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引用次数: 0
The Effect of Adding Bee Pollen on The Sexual Efficiency of Quail Males 添加蜂花粉对雄性鹌鹑性效率的影响
Pub Date : 2022-11-15 DOI: 10.21608/javs.2022.164168.1180
M. Yonis, A. Hassan
This study aims to determine the role of bee pollen in improving the reproductive efficiency of quail males raised at high density. The total number was 288 birds at 7 weeks old, randomly distributed to 6 experimental groups. The 1 st group was placed at a rate of 21 birds / m 2 , 2 nd group was placed at a rate of 21 birds / m 2 and addition of bee pollen 20 g/kg feed, the 3 rd group was at a rate of 21 birds / m 2 addition of bee pollen 30 g/kg feed, the 4 th group was high density 75 birds / m 2 , 5 th group was high density75 birds/ m 2 and supplemented with bee pollen 20 g/kg feed, and the 6 th group was high density 75 birds/m 2 and supplemented with bee pollen 30 g/kg feed. The results showed that adding 20g and 30g of bee pollen to the diet of 21 birds caused a significant increase in testosterone hormone. In contrast, the high density of birds caused a significant decrease in testosterone, sperm count and live sperm accompanied by an increase in the dead and deformed sperm. Furthermore, decrease in body weight. Adding 20g and 30g of bee pollen to the high density of birds resulted in a significant increase in testosterone, sperm count, and live sperm accompanied by a reduction in dead and deformed sperm and an increase in body weight. The study concluded that the high density of the birds' numbers had a negative impact on the bird's reproductive efficiency and body weight while adding bee pollen to the diet improved quail male body weight and reproductive efficiency.
本研究旨在确定蜂花粉在提高高密度饲养的鹌鹑雄性繁殖效率方面的作用。7周龄时的总数量为288只,随机分为6个实验组。第1组以21只/m 2的速度放置,第2组以21两只/m 2的速率放置并添加蜂花粉20 g/kg饲料,第3组以2 1只/m 2添加蜂花粉30 g/kg饲料,第6组为高密度75只/m2,蜂花粉30g/kg饲料。结果表明,在21只鸟类的日粮中分别添加20克和30克蜂花粉,可显著提高雄性激素水平。相比之下,高密度的鸟类导致睾酮、精子数量和活精子显著减少,同时死亡和畸形精子增加。此外,体重减轻。在高密度的鸟类中添加20克和30克蜂花粉,可以显著增加睾酮、精子数量和活精子,同时减少死亡和畸形精子,增加体重。研究得出结论,高密度的鸟类数量对鹌鹑的繁殖效率和体重产生了负面影响,而在饮食中添加蜂花粉可以提高鹌鹑雄性的体重和繁殖效率。
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引用次数: 1
Sero-prevalence of Border Disease Virus Antibodies in Recently Introduced Dorper Sheep Flocks at Debre Birhan Agricultural Research Center, Ethiopia 埃塞俄比亚Debre Birhan农业研究中心新近引进的杜珀羊群中边境病病毒抗体的血清流行率
Pub Date : 2022-10-31 DOI: 10.21608/javs.2022.155122.1171
Chekol Getahun, Tadiwos Dessie, Eridachew Tesema, Enyiew Alamirew
A study was conducted to investigate the serological prevalence of Border Disease Virus (BDV) antibodies in newly introduced Dorper sheep flocks at Debre Birhan Agricultural Research Center (DBARC), Amhara Region, Ethiopia. Blood samples were collected from the jugular vein of 186 sheep in the research center and the serum was decanted and sent to the National Animal Health Diagnostic and Investigation Center, Sebeta, Ethiopia for laboratory examination. All serum samples were examined using blocking ELISA test with a sensitivity of 94.3% and specificity of 93.7% to detect antibodies for BDV. Of these, 55 (29.57%) tested sero-positive and the rest 131 (70.43%) tested sero-negative for BDV antibodies. The sero-prevalence of sheep born in the research center was found at 23.97% (29/121) whereas the sero-prevalence of those sheep imported directly from abroad was found at 40% (26/65). In this research, a higher prevalence of BDV antibodies were recorded in the imported sheep than in the sheep which are born in the research center and the difference in prevalence between the two groups was found statistically significant (P<0.05). Therefore, it is of utmost importance to study the status of the disease from indigenous breeds of sheep and utilize more sensitive tests such as RT-PCR.
在埃塞俄比亚阿姆哈拉地区Debre Birhan农业研究中心(DBARC)进行了一项研究,以调查新引进的Dorper绵羊群中边境病病毒(BDV)抗体的血清学流行率。从研究中心186只绵羊的颈静脉采集血样,倾析血清,送往埃塞俄比亚塞贝塔的国家动物健康诊断和调查中心进行实验室检查。所有血清样品均采用阻断ELISA法检测BDV抗体,灵敏度为94.3%,特异性为93.7%。其中,55人(29.57%)的血清BDV抗体检测呈阳性,其余131人(70.43%)的血清抗体检测呈阴性。研究中心出生的绵羊的血清患病率为23.97%(29/121),而直接从国外进口的绵羊的血清学患病率为40%(26/65)。在本研究中,进口绵羊的BDV抗体患病率高于研究中心出生的绵羊,两组之间的患病率差异具有统计学意义(P<0.05)。因此,研究本地绵羊品种的疾病状况并利用更敏感的测试如RT-PCR是至关重要的。
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引用次数: 0
Struvite Bladder Stone in a Cat with Hyperthyroidism 甲状腺功能亢进猫的Struite膀胱结石
Pub Date : 2022-09-22 DOI: 10.21608/javs.2022.155293.1172
E. Koral, C. Er, A. Barut
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引用次数: 0
Some Biochemical changes induced by Toxic Effects of Sulfur in Mice 硫的毒性作用引起小鼠的一些生化变化
Pub Date : 2022-09-13 DOI: 10.21608/javs.2022.155557.1173
F. Hassan, B. Al-baggou
{"title":"Some Biochemical changes induced by Toxic Effects of Sulfur in Mice","authors":"F. Hassan, B. Al-baggou","doi":"10.21608/javs.2022.155557.1173","DOIUrl":"https://doi.org/10.21608/javs.2022.155557.1173","url":null,"abstract":"","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47621815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of Histopathological Changes Induced by Amitraz in Rats 阿米特拉兹致大鼠组织病理学改变的鉴定
Pub Date : 2022-09-09 DOI: 10.21608/javs.2022.154375.1169
Zainab. J. Mohammed, Rizgar Nabi, Y. Mousa, M. Mahmood
{"title":"Identification of Histopathological Changes Induced by Amitraz in Rats","authors":"Zainab. J. Mohammed, Rizgar Nabi, Y. Mousa, M. Mahmood","doi":"10.21608/javs.2022.154375.1169","DOIUrl":"https://doi.org/10.21608/javs.2022.154375.1169","url":null,"abstract":"","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47724226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessment of the Knowledge, Attitude and Practices of Dog Owners on Rabies in Ilorin, Kwara State, Nigeria 尼日利亚夸拉州伊洛林市养狗人对狂犬病知识、态度和做法的评估
Pub Date : 2022-09-08 DOI: 10.21608/javs.2022.154332.1168
J. Aiyedun, A. Darajat, Daodu B.O., Oludairo O.O., I. Olorunshola, Nwoha R.I.O., I. Oluboyede
{"title":"Assessment of the Knowledge, Attitude and Practices of Dog Owners on Rabies in Ilorin, Kwara State, Nigeria","authors":"J. Aiyedun, A. Darajat, Daodu B.O., Oludairo O.O., I. Olorunshola, Nwoha R.I.O., I. Oluboyede","doi":"10.21608/javs.2022.154332.1168","DOIUrl":"https://doi.org/10.21608/javs.2022.154332.1168","url":null,"abstract":"","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43570943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Journal of Applied Veterinary Sciences
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