Pub Date : 2023-02-16DOI: 10.21608/javs.2023.178047.1198
A. Hassenin, Uzema Durrani
{"title":"Seroprevalence and Evidence of Borrelia burgdorferi Infection in Stray Dogs at AS- Sharqia Province, Egypt","authors":"A. Hassenin, Uzema Durrani","doi":"10.21608/javs.2023.178047.1198","DOIUrl":"https://doi.org/10.21608/javs.2023.178047.1198","url":null,"abstract":"","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49066793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-16DOI: 10.21608/javs.2023.174366.1193
D. Adah, A. Adah, N. Furo, Isiaku Abdulmajeed
{"title":"Ameliorative Effects of Elaeis guineensis Oil on Water Quality, Hematological and Biochemical Parameters of Clarias gariepinus under Transport Condition","authors":"D. Adah, A. Adah, N. Furo, Isiaku Abdulmajeed","doi":"10.21608/javs.2023.174366.1193","DOIUrl":"https://doi.org/10.21608/javs.2023.174366.1193","url":null,"abstract":"","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47836532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-23DOI: 10.21608/javs.2022.174716.1192
Azza M. Ezzeldin, E. Bashandy, Zakia A. M. Ahmed, T. Ismail
{"title":"Descriptive Epidemiology and Associated Risk Factors of Lumpy Skin Disease in Egypt during 2006-2018","authors":"Azza M. Ezzeldin, E. Bashandy, Zakia A. M. Ahmed, T. Ismail","doi":"10.21608/javs.2022.174716.1192","DOIUrl":"https://doi.org/10.21608/javs.2022.174716.1192","url":null,"abstract":"","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47075344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-14DOI: 10.21608/javs.2022.172532.1189
M. Kamel, Yaseein Soubhi Hussein
Camels play an important role in social life and the economy. So, the current study was planned to detect the blood constituents and milk components of Maghrebi She-Camel in different parties. Thirty pregnant she-camels were inseminated and became pregnant in this study during the first quarter of 2019; thirteen pregnant she-camel managed to give calving successfully during March 2020. According to multiple parities, they were classified into three groups; (1-2 parities), (3-4 parities) and (5-6 parities). Blood measurements included hematology, substances, ions and milk measurements included yield and components. No significant differences between all groups for all hematological measurements. The overall mean of daily milk production was significantly higher after eight weeks of lactation than at the beginning of lactation. No significant results were shown in milk fat, lactose and protein neither parties nor lactation period. Milk total solid significantly was in its lowest values eight days of parturition for the first and second parties and was at its highest values for the third and fourth parties. A significant difference in ash% was found between experimental parties for the first eight days of lactation and after eight weeks. In general, those insignificant differences in blood parameters, milk productivity and composition might indicate animal health and stability.
{"title":"Changes in Hematopoietic Levels and Milk Components during the First Weeks after Labor in the Maghrebi She-Camel","authors":"M. Kamel, Yaseein Soubhi Hussein","doi":"10.21608/javs.2022.172532.1189","DOIUrl":"https://doi.org/10.21608/javs.2022.172532.1189","url":null,"abstract":"Camels play an important role in social life and the economy. So, the current study was planned to detect the blood constituents and milk components of Maghrebi She-Camel in different parties. Thirty pregnant she-camels were inseminated and became pregnant in this study during the first quarter of 2019; thirteen pregnant she-camel managed to give calving successfully during March 2020. According to multiple parities, they were classified into three groups; (1-2 parities), (3-4 parities) and (5-6 parities). Blood measurements included hematology, substances, ions and milk measurements included yield and components. No significant differences between all groups for all hematological measurements. The overall mean of daily milk production was significantly higher after eight weeks of lactation than at the beginning of lactation. No significant results were shown in milk fat, lactose and protein neither parties nor lactation period. Milk total solid significantly was in its lowest values eight days of parturition for the first and second parties and was at its highest values for the third and fourth parties. A significant difference in ash% was found between experimental parties for the first eight days of lactation and after eight weeks. In general, those insignificant differences in blood parameters, milk productivity and composition might indicate animal health and stability.","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46271316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-14DOI: 10.21608/javs.2022.169619.1186
E. Koral, M. Durgut
The case includes a 7-month-old puppy poodle applied to a private hospital for weakness, tremors and seizure attacks. Clinical examination findings were normal. From blood samples, biochemical parameter measurements were carried out. The values of alkaline phosphatase (ALP), alanine aminotransferase (ALT), ammonia and fasting serum bile acids were high and the blood urea nitrogen (BUN) value was low. Ultrasonographic examination, shunted vein in the liver to the vena cava caudalis, that is, colour Doppler observed the extrahepatic shunt and turbulent flow in this shunted. Depending on clinical, laboratory (hemogram and biochemistry) and ultrasonographic observations, portosystemic shunt (PSS) was diagnosed and controlled one month after the treatment was recommended. The medical treatment included a hepatic formula diet (liver care), lactulose 0.5 ml/kg three times a day, metronidazole 15 mg/kg twice a day, S-adenosyl Methionine 15 mg/kg once a day, 400 international unite (IU) vitamin E once a day for 30 days. When turbulent flow is observed in the shunted vein in the liver to vena cava caudalis, ultrasound examination with color Doppler can help diagnose portosystemic shunt. After the treatment, clinical improvement was observed and clinical symptoms of hepatic encephalopathy including seizures and tremors, disappeared completely. Determination of turbulent flow with colour Doppler and decreased portal flow velocity with portal hypertension with PW-Doppler ultrasonography are important for the diagnosis of the portosystemic shunt. It was concluded that medical treatments might help before surgical treatments in portosystemic shunts. ـــــــــــــــــــــــــــــــــــــــــ
{"title":"Portosystemic Shunt in A Puppy Poodle and Medical Treatment: Case report","authors":"E. Koral, M. Durgut","doi":"10.21608/javs.2022.169619.1186","DOIUrl":"https://doi.org/10.21608/javs.2022.169619.1186","url":null,"abstract":"The case includes a 7-month-old puppy poodle applied to a private hospital for weakness, tremors and seizure attacks. Clinical examination findings were normal. From blood samples, biochemical parameter measurements were carried out. The values of alkaline phosphatase (ALP), alanine aminotransferase (ALT), ammonia and fasting serum bile acids were high and the blood urea nitrogen (BUN) value was low. Ultrasonographic examination, shunted vein in the liver to the vena cava caudalis, that is, colour Doppler observed the extrahepatic shunt and turbulent flow in this shunted. Depending on clinical, laboratory (hemogram and biochemistry) and ultrasonographic observations, portosystemic shunt (PSS) was diagnosed and controlled one month after the treatment was recommended. The medical treatment included a hepatic formula diet (liver care), lactulose 0.5 ml/kg three times a day, metronidazole 15 mg/kg twice a day, S-adenosyl Methionine 15 mg/kg once a day, 400 international unite (IU) vitamin E once a day for 30 days. When turbulent flow is observed in the shunted vein in the liver to vena cava caudalis, ultrasound examination with color Doppler can help diagnose portosystemic shunt. After the treatment, clinical improvement was observed and clinical symptoms of hepatic encephalopathy including seizures and tremors, disappeared completely. Determination of turbulent flow with colour Doppler and decreased portal flow velocity with portal hypertension with PW-Doppler ultrasonography are important for the diagnosis of the portosystemic shunt. It was concluded that medical treatments might help before surgical treatments in portosystemic shunts. ـــــــــــــــــــــــــــــــــــــــــ","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44154684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-13DOI: 10.21608/javs.2022.171742.1187
G. S.N., G. S., Jiddah A. A., A. A.
P.M.B
{"title":"The effect of Turmeric (Curcuma longa) Powder on Serum Biochemical parameters of Broilers","authors":"G. S.N., G. S., Jiddah A. A., A. A.","doi":"10.21608/javs.2022.171742.1187","DOIUrl":"https://doi.org/10.21608/javs.2022.171742.1187","url":null,"abstract":"P.M.B","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43038487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-13DOI: 10.21608/javs.2022.173225.1191
Ismael Idrees, G. Taqa, Saba Ibrahim
Acetylcholinesterase is required as an enzyme to counteract the effects of acetylcholine. The aim of the study is to assess how amitriptyline and Ashwagandha affect the acetylcholinesterase gene in rat salivary glands. Forty healthy albino rats were divided randomly into four equal groups: Group I (control) received distilled water for 30 days. Group II received amitriptyline (10mg/kg) for 30 days. Group III received ashwagandha watery root extract (200mg/kg) orally for 30 days and Group IV received the combination of amitriptyline orally and ashwagandha root extract orally for 30 days. Rats in each group were sacrificed after day 30 and salivary glands were dissected for measurement of the acetylcholinesterase gene using a Polymerase Chain Reaction technique (PCR). Acetylcholinesterase gene measurements reveal an increase in groups treated with amitriptyline alone (1.55±0.11) and in the group treated with a combination of amitriptyline with Ashwagandha (1.92±0.16), in comparison with the control group. There were no discernible differences between the Ashwagandha treated group (1.073± 0.25) compared to the control group (0.76±0.19).In conclusion , Amitriptyline alone and, when combined with Ashwagandha cause transcription of the acetylcholinesterase gene. ـــــــــــــــــــــــــــــــــــــــــ
{"title":"Acetylcholine Esterase Gene Expression in Salivary Glands of Albino Rats after Treatment with amitriptyline or/and Ashwagandha","authors":"Ismael Idrees, G. Taqa, Saba Ibrahim","doi":"10.21608/javs.2022.173225.1191","DOIUrl":"https://doi.org/10.21608/javs.2022.173225.1191","url":null,"abstract":"Acetylcholinesterase is required as an enzyme to counteract the effects of acetylcholine. The aim of the study is to assess how amitriptyline and Ashwagandha affect the acetylcholinesterase gene in rat salivary glands. Forty healthy albino rats were divided randomly into four equal groups: Group I (control) received distilled water for 30 days. Group II received amitriptyline (10mg/kg) for 30 days. Group III received ashwagandha watery root extract (200mg/kg) orally for 30 days and Group IV received the combination of amitriptyline orally and ashwagandha root extract orally for 30 days. Rats in each group were sacrificed after day 30 and salivary glands were dissected for measurement of the acetylcholinesterase gene using a Polymerase Chain Reaction technique (PCR). Acetylcholinesterase gene measurements reveal an increase in groups treated with amitriptyline alone (1.55±0.11) and in the group treated with a combination of amitriptyline with Ashwagandha (1.92±0.16), in comparison with the control group. There were no discernible differences between the Ashwagandha treated group (1.073± 0.25) compared to the control group (0.76±0.19).In conclusion , Amitriptyline alone and, when combined with Ashwagandha cause transcription of the acetylcholinesterase gene. ـــــــــــــــــــــــــــــــــــــــــ","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47416003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.21608/javs.2022.168375.1184
A. Adah, J. Ayo, D. Adah
The dromedary camel is mainly found in the tropics of Africa, the Middle East, and the Indian sub-content, where it is of great importance to nomadic and rural communities to provide high-quality animal protein in the form of milk and meat and as a means of transportation. In the Arabian Gulf region, the camel gained popularity and importance recently as a racing animal. The camel is well adapted to harsh environments characterized by inadequate water and vegetation, high ambient temperature
{"title":"Unique Physiological and Behavioural Adaptive Features of The One-Humped Camel (Camelus dromedarius) to Arid Environments","authors":"A. Adah, J. Ayo, D. Adah","doi":"10.21608/javs.2022.168375.1184","DOIUrl":"https://doi.org/10.21608/javs.2022.168375.1184","url":null,"abstract":"The dromedary camel is mainly found in the tropics of Africa, the Middle East, and the Indian sub-content, where it is of great importance to nomadic and rural communities to provide high-quality animal protein in the form of milk and meat and as a means of transportation. In the Arabian Gulf region, the camel gained popularity and importance recently as a racing animal. The camel is well adapted to harsh environments characterized by inadequate water and vegetation, high ambient temperature","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45294229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-01DOI: 10.21608/javs.2022.167692.1183
H. Sorour, H. Badr, M. Abdelaty, H. Roshdy, A. Mohammed, M. AbdelRahman
Salmonellosis is a major global pathogen in the poultry industry and is a significant public health concern. Ducks are known to be carriers of Salmonella . Therefore, monitoring salmonellosis is the most important strategy for preventing the disease. An experimental design was planned to study the pathogenicity of two Salmonella strains. One hundred and fifty chicks were divided into three groups; group one was inoculated with the Salmonella enteritidis strain, group two was inoculated with the Salmonella typhimurium strain, and group three was UN inoculated. Symptoms, postmortem lesions and mortality rate were recorded. The chick growth performance parameters were also determined. Using ANOVA for statistical analysis, there was a significant difference in body weight, body gain, feed consumption, and feed conversion ratio between the two infected groups and the blank group (uninoculated group). In this study, the prevalence of Salmonella enteritidis was (1.73%) and Salmonella typhimurium (0.43%) in imported ducklings in Egypt. Both Salmonella strains were subjected to an antimicrobial sensitivity test. It showed that Salmonella enteritidis had a 60% antimicrobial resistance profile and Salmonella typhimurium had a 20% antimicrobial resistance profile. Furthermore, genotypic characterization was performed and the seven virulence genes(stn, avrA, sopB, ompF, invA, Mgtc, Ssaq) were found. New pathological lesions of Salmonella infection were discovered, such as skull hemorrhage at 3 days and 6 days of age, and a liver similar to a button shape in necropsied infected chicks with Salmonella typhimurium at 21 days of age. Furthermore, hemorrhagic spots were observed on the duodenum. In the presence of Salmonella , Clostridium perferingens was discovered in a bacteriological investigation of duodenal lesions samples from infected chicks. At 30 days of age, administration of acetic acid (1%) as an alternative tool for controlling Salmonella . In conclusion, salmonellosis is a risk factor for necrotic enteritis, and using acetic acid to eliminate salmonella infection is insufficient.
{"title":"Virulence Range and New Pathological Pictures of Salmonella enteridits and Salmonella typhimurium Isolated from Ducklings in Experimental Infected Chicks","authors":"H. Sorour, H. Badr, M. Abdelaty, H. Roshdy, A. Mohammed, M. AbdelRahman","doi":"10.21608/javs.2022.167692.1183","DOIUrl":"https://doi.org/10.21608/javs.2022.167692.1183","url":null,"abstract":"Salmonellosis is a major global pathogen in the poultry industry and is a significant public health concern. Ducks are known to be carriers of Salmonella . Therefore, monitoring salmonellosis is the most important strategy for preventing the disease. An experimental design was planned to study the pathogenicity of two Salmonella strains. One hundred and fifty chicks were divided into three groups; group one was inoculated with the Salmonella enteritidis strain, group two was inoculated with the Salmonella typhimurium strain, and group three was UN inoculated. Symptoms, postmortem lesions and mortality rate were recorded. The chick growth performance parameters were also determined. Using ANOVA for statistical analysis, there was a significant difference in body weight, body gain, feed consumption, and feed conversion ratio between the two infected groups and the blank group (uninoculated group). In this study, the prevalence of Salmonella enteritidis was (1.73%) and Salmonella typhimurium (0.43%) in imported ducklings in Egypt. Both Salmonella strains were subjected to an antimicrobial sensitivity test. It showed that Salmonella enteritidis had a 60% antimicrobial resistance profile and Salmonella typhimurium had a 20% antimicrobial resistance profile. Furthermore, genotypic characterization was performed and the seven virulence genes(stn, avrA, sopB, ompF, invA, Mgtc, Ssaq) were found. New pathological lesions of Salmonella infection were discovered, such as skull hemorrhage at 3 days and 6 days of age, and a liver similar to a button shape in necropsied infected chicks with Salmonella typhimurium at 21 days of age. Furthermore, hemorrhagic spots were observed on the duodenum. In the presence of Salmonella , Clostridium perferingens was discovered in a bacteriological investigation of duodenal lesions samples from infected chicks. At 30 days of age, administration of acetic acid (1%) as an alternative tool for controlling Salmonella . In conclusion, salmonellosis is a risk factor for necrotic enteritis, and using acetic acid to eliminate salmonella infection is insufficient.","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45645742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-11-15DOI: 10.21608/javs.2022.159573.1177
S. Ameen, A. Selim, Mohamed Tarek, A. Zanaty, M. AboElkhair, A. Bazid
Despite widespread immunizations, infectious bronchitis (IB) remains a significant issue in the Egyptian poultry industry. Multiple IBV genotypes, GI-1, GI-13, GI-16, and GI-23 have been continually circulating among chicken flocks in Egypt, inducing a substantial economic loss to the poultry sector. In addition, live attenuated vaccines representing classical and variant strains can control IBV in Egypt, mainly H120 and 793B. The H120 vaccine is widely spread and offers inadequate protection against heterotypic IBVs in the field. Therefore, a homologous live-attenuated VAR2 vaccine was developed from the Egyptian variant-2 strain Eg/1212B/2012.IB. Variant II vaccine protects against the homologous IBV challenge under experimental and field circumstances. In this study, an experimental trial was performed to simulate field practices such as heterologous vaccination of day-old specific pathogen-free chicks with IBV H120 vaccine (representing GI-1) and IB Var 2 vaccine (representing GI-23). The current study aimed to determine the existence of nucleotide and amino acid variations within the S gene in isolated viruses following ten passages in the same bird. The deduced amino acid sequence of the S gene indicated viruses isolated from the 6 th and 10 th passages were identical and shared (96 %) and (83 %) identities with the IB variant II vaccine and H120, respectively. However, amino acid substitutions were observed at 26 positions in the N terminal domain (S1) and S2 is conserved compared to IB Var 2 vaccine. Most amino acid modifications occurred in the receptor binding domain (RBD) of the S1 gene. HVR2 has seven amino acid changes compared to the IB Var II vaccine. Isolates of P6 and P10 lacked IBV glycosylation site at position 139 which was detected in IBV/EG/1212B/2012 as well as IB variant II vaccine. The study also revealed no evidence of recombination between the two used live vaccines. ـــــــــــــــــــــــــــــــــــــــــ
尽管广泛的免疫接种，传染性支气管炎(IB)仍然是埃及家禽业的一个重要问题。多种IBV基因型GI-1、GI-13、GI-16和GI-23在埃及的鸡群中持续传播，给家禽业造成了巨大的经济损失。此外，代表经典毒株和变异毒株的减毒活疫苗可以控制埃及的IBV，主要是H120和793B。H120疫苗广泛传播，在野外对异型ibv提供的保护不足。因此，从埃及变体-2株Eg/1212B/2012.IB中开发出同源的VAR2减毒活疫苗。II型变异体疫苗在实验和田间环境下可防止同源IBV的攻击。在这项研究中，进行了一项模拟野外实践的实验试验，例如用IBV H120疫苗(代表GI-1)和IBV Var 2疫苗(代表GI-23)异种接种日龄特异性无病原体雏鸡。目前的研究旨在确定在同一只鸟中经过10次传代后分离病毒S基因内核苷酸和氨基酸变异的存在。S基因的氨基酸序列表明，从第6代和第10代分离的病毒分别与IB变型II疫苗和H120具有相同的(96%)和(83%)的同源性。然而，与IB Var 2疫苗相比，在N端结构域(S1)的26个位置观察到氨基酸取代，S2是保守的。大多数氨基酸修饰发生在S1基因的受体结合域(RBD)。与IB Var II疫苗相比，HVR2有7个氨基酸变化。分离株P6和P10缺乏在IBV/EG/1212B/2012和IB变体II疫苗中检测到的139位IBV糖基化位点。该研究还显示，没有证据表明两种使用过的活疫苗之间存在重组。ـــــــــــــــــــــــــــــــــــــــــ
{"title":"Genetic Changes of S Gene during Co-inoculation of Two Infectious Bronchitis Virus Vaccines in SPF Chicks","authors":"S. Ameen, A. Selim, Mohamed Tarek, A. Zanaty, M. AboElkhair, A. Bazid","doi":"10.21608/javs.2022.159573.1177","DOIUrl":"https://doi.org/10.21608/javs.2022.159573.1177","url":null,"abstract":"Despite widespread immunizations, infectious bronchitis (IB) remains a significant issue in the Egyptian poultry industry. Multiple IBV genotypes, GI-1, GI-13, GI-16, and GI-23 have been continually circulating among chicken flocks in Egypt, inducing a substantial economic loss to the poultry sector. In addition, live attenuated vaccines representing classical and variant strains can control IBV in Egypt, mainly H120 and 793B. The H120 vaccine is widely spread and offers inadequate protection against heterotypic IBVs in the field. Therefore, a homologous live-attenuated VAR2 vaccine was developed from the Egyptian variant-2 strain Eg/1212B/2012.IB. Variant II vaccine protects against the homologous IBV challenge under experimental and field circumstances. In this study, an experimental trial was performed to simulate field practices such as heterologous vaccination of day-old specific pathogen-free chicks with IBV H120 vaccine (representing GI-1) and IB Var 2 vaccine (representing GI-23). The current study aimed to determine the existence of nucleotide and amino acid variations within the S gene in isolated viruses following ten passages in the same bird. The deduced amino acid sequence of the S gene indicated viruses isolated from the 6 th and 10 th passages were identical and shared (96 %) and (83 %) identities with the IB variant II vaccine and H120, respectively. However, amino acid substitutions were observed at 26 positions in the N terminal domain (S1) and S2 is conserved compared to IB Var 2 vaccine. Most amino acid modifications occurred in the receptor binding domain (RBD) of the S1 gene. HVR2 has seven amino acid changes compared to the IB Var II vaccine. Isolates of P6 and P10 lacked IBV glycosylation site at position 139 which was detected in IBV/EG/1212B/2012 as well as IB variant II vaccine. The study also revealed no evidence of recombination between the two used live vaccines. ـــــــــــــــــــــــــــــــــــــــــ","PeriodicalId":15040,"journal":{"name":"Journal of Applied Veterinary Sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42320425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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