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Antioxidant Action of Phytic Acid in Processed Fish Waste and Cooked Fish Sausages 加工鱼渣和熟鱼肠中植酸的抗氧化作用
4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-09-27 DOI: 10.1080/10498850.2023.2260825
Taida Juliana Adorian, Luciane Ferreira de Moura, Ana Carolina Kohlrausch Klinger, Ana Betine Beutinger Bender, Caroline Sefrin Speroni, Fernanda Rodrigues Goulart Ferrigolo, Naglezi DeMenezes Lovatto, Leila Picolli da Silva
ABSTRACTThe study evaluated the efficiency of phytic acid (PA) for stabilization of processed fish waste (PFW – ventral muscle and vertebral column without fins) and its use as a substitute for pork in cooked sausages. The PA and sodium erythorbate (0.05%) was compared by quantifying hydroperoxides. PFW stabilized with 0.05% PA was used to replace pork (0, 25, 50, and 75% PFW) sausage formulation. All formulations showed a difference in texture in comparison to control, which had higher levels of hydroperoxides. Phytic acid has antioxidant action on PFW, optimized in 0.05%. PFW can substitute up to 25% for pork in cooked sausages.KEYWORDS: Aquaculturefish productmechanically separated meatoxidative stability AcknowledgmentsThe authors are thankful to the financial assistance provided by the Foundation Coordination for the Improvement of Higher Education Personnel (CAPES) by means of a Master’s degree scholarship, and to the National Council for Scientific and Technological Development (CNPq) for granting a research productivity scholarship to Professor Leila Picolli da Silva. They are also grateful to the companies Bremil and Ingal Food for supplying antioxidants.Author contributionsLFM: carried out the research, laboratory and statistical analyses; LPS: professor who guided the research and helped in writing the manuscript; TJA: data processing and manuscript writing; ACKK: data processing and manuscript writing; ABBB: assisted in laboratory analyses; CSS: assisted in laboratory analyses; FRGF: assisted in laboratory analyses; NML: assisted in laboratory analyses.Disclosure statementNo potential conflict of interest was reported by the author(s).Ethics approvalThe study design was conducted with the ethical approval of all relevant bodies in Universidad Federal de Santa Maria, Brazil.Data availability statementhttps://doi.org/10.17632/654y68mwpc.1
摘要本研究评价了植酸(PA)对加工鱼废物(PFW -腹肌和无鳍的脊柱)的稳定性及其在熟香肠中替代猪肉的应用。通过对过氧化氢的定量分析,比较了PA和赤藓酸钠(0.05%)的含量。用0.05% PA稳定的PFW代替猪肉(0、25、50、75% PFW)香肠配方。与氢过氧化物含量较高的对照组相比,所有配方的质地都有所不同。植酸对PFW有抗氧化作用,最佳浓度为0.05%。在煮熟的香肠中,PFW可以代替高达25%的猪肉。作者感谢高等教育人员发展协调基金会(CAPES)提供的硕士学位奖学金和国家科学技术发展委员会(CNPq)为Leila Picolli da Silva教授提供的研究生产力奖学金。他们也感谢brremil和Ingal食品公司提供抗氧化剂。slfm:进行研究、实验室和统计分析;LPS:指导研究并帮助撰写手稿的教授;TJA:数据处理和稿件撰写;ACKK:数据处理和稿件撰写;协助实验室分析;CSS:协助实验室分析;FRGF:协助实验室分析;协助实验室分析。披露声明作者未报告潜在的利益冲突。伦理批准本研究设计是在巴西圣玛丽亚联邦大学所有相关机构的伦理批准下进行的。数据可用性声明://doi.org/10.17632/654y68mwpc.1
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引用次数: 0
Immunohistochemical Evaluation of Histamine Levels and Hygienic Aspect of Little Tunny (Euthynnus alletteratus) Musculature at Different Environment Time and Temperature 不同环境时间和温度下小金枪鱼肌肉组织组胺水平和卫生状况的免疫组化评价
4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-09-26 DOI: 10.1080/10498850.2023.2259900
Wafaa A. Mohamed, Eman I. Hassanen, Hayam A. Mansour, Mahmoud A. Mahmoud
ABSTRACT We investigated the effect of storage time and temperature on muscular histamine (HIS) contents in little tunny (LT). Fifty LT were preserved at various temperatures (0- 21°C). We collected muscles from several areas at 0-, 6-, 12-, 18-, and 24 h post-fishing for further examination. Throughout storage time and temperature, there was a significant increase in fish deterioration, HIS levels, and HFB count in abdominal > tail > dorsal muscle. Vitek2 gave 99% confidence identity of the highly histaminergic strains Lelliottia amnigena-1 and Acinetobacter haemolyticus. There was a positive correlation between HIS levels, HFB counts, and microscopic lesion scores during storage.KEYWORDS: Histaminelittle tunnyLelliottia amnigena-1Acinetobacter haemolyticus Author contributionsResearch concept: WAM, EIH, HAM, MAM; Fish sampling: WAM; Sensory evaluation: HAM, WAM, MAM; Bacteriological examination: HAM, WAM; Muscular autolysis scoring: WAM, EIH, MAM; Determination of Histamine contents, immunohistochemistry and statistical analysis: WAM, EIH; Drafting manuscript: WAM, EIH, HAM, MAM; Revision and final approval of this version for publication: WAM, EIH, HAM, MAM; Teamwork leader: MAM.Disclosure statementNo potential conflict of interest was reported by the author(s).Data availability statementThe authors confirm that all the data supporting the findings of this study are available within this article.Supplementary dataSupplemental data for this article can be accessed online at https://doi.org/10.1080/10498850.2023.2259900.
摘要本试验研究了贮藏时间和温度对小金枪鱼肌肉组胺(HIS)含量的影响。50个LT在不同温度(0- 21℃)下保存。我们在捕鱼后0、6、12、18和24小时收集了几个区域的肌肉,以便进一步检查。在不同的贮藏时间和温度下,鱼的劣化程度、HIS水平和腹部>尾部>背部肌肉的HFB计数显著增加。Vitek2对高组胺能菌株水蛭-1和溶血不动杆菌的鉴定置信度为99%。贮藏期间HIS水平、HFB计数和显微病变评分呈正相关。关键词:组胺小隧道溶血不动杆菌研究概念:WAM, EIH, HAM, MAM;鱼类取样:WAM;感官评价:HAM, WAM, MAM;细菌学检查:HAM、WAM;肌肉自溶评分:WAM、EIH、MAM;组胺含量测定、免疫组化及统计分析:WAM、EIH;起草稿:WAM, EIH, HAM, MAM;本版本修订并最终批准发布:WAM, EIH, HAM, MAM;团队领导:MAM。披露声明作者未报告潜在的利益冲突。数据可用性声明作者确认,支持本研究结果的所有数据均可在本文中获得。本文的补充数据可以在线访问https://doi.org/10.1080/10498850.2023.2259900。
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引用次数: 0
Effects of High Pressure Treatment and Chitosan Coating on Selected Enzymatic Activities and Quality Indices of Barramundi ( Lates calcarifer ) Muscle 高压处理和壳聚糖包衣对梭鱼肌肉部分酶活性和品质指标的影响
4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-09-24 DOI: 10.1080/10498850.2023.2260799
Binh Q. Truong, Roman Buckow, Minh H. Nguyen
ABSTRACTThree different treatments: pressurization at 200 MPa for 3 min (HPP), chitosan coating and then pressurization (CHPP), and chitosan coating (CHI) were used on barramundi muscle to investigate quality indices and enzymatic activities during chilled storage (4 ± 1°C). HPP significantly improved texture and reduced proteolytic activity and drip loss of barramundi muscle compared to CHI. Chitosan coating significantly reduced microbial growth as compared to HPP. CHPP treatment improved texture and reduced protease activity, total volatile basic nitrogen formation, drip loss, and microbial growth, but it accelerated lipid oxidation. Thus, HPP is the most favorable for improving barramundi muscle quality during chilled storage up to 23 days.KEYWORDS: High-pressure processingbarramundichitosanchilled storageenzymatic activities Disclosure statementNo potential conflict of interest was reported by the author(s).Data availability statementData are available within the article or its supplementary materials.
摘要采用200 MPa加压3 min (HPP)、壳聚糖包覆后加压(CHPP)和壳聚糖包覆(CHI) 3种不同处理方法,研究了在4±1°C冷藏条件下鲱鱼肌肉的品质指标和酶活性。与CHI相比,HPP显著改善了barramundi肌肉的质地,降低了蛋白质水解活性和滴水损失。与HPP相比,壳聚糖涂层显著降低了微生物的生长。CHPP处理改善了籽粒织质,降低了蛋白酶活性、总挥发性碱性氮形成、滴漏损失和微生物生长,但加速了脂质氧化。因此,在冷藏23天内,HPP对改善梭鱼肌肉品质最为有利。关键词:高压加工barramundichitsanchylstorage酶活性披露声明作者未报告潜在的利益冲突。数据可用性声明数据可在文章或其补充材料中获得。
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引用次数: 0
Identification of Endogenous and Artificial Formaldehyde Biomarkers in Bombay Duck ( Harpodon nehereus ) Using 1H NMR-Based Metabolomics Approach 利用基于1H核磁共振的代谢组学方法鉴定孟买鸭(Harpodon nehereus)内源性和人工甲醛生物标志物
4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-09-21 DOI: 10.1080/10498850.2023.2259364
Giri Rohmad Barokah, Tati Nurhayati, Hedi Indra Januar, Agoes Jacoeb
ABSTRACTFormaldehyde can occur endogenously in fisheries products by enzymatic processes. However, the practice of illegally adding artificial formaldehyde still occurs in several developing countries, including Indonesia. The aim of this study is to identify the biomarkers used to distinguish endogenous from artificial formaldehyde in Bombay duck (Harpodon nehereus) as a sample fisheries product. The 1H NMR-based metabolomic approach was used to identify metabolites in samples. Metabolite analysis with proton NMR detected 11 metabolites in Bombay duck fillet samples (i.e. lactic acid, acetic acid, trimethylamine oxide (TMAO), trimethylamine (TMA), dimethylamine, lysine, anserine, inosine, inosine-5’-monophospate, and hypoxanthine). The results of the study showed that three metabolites in this list, namely trimethylamine oxide (TMAO), trimethylamine (TMA), and lysine, can be used as indicators to distinguish endogenous formaldehyde from artificial formaldehyde in Bombay duck fish.KEYWORDS: BiomarkersFormaldehydeHarpodon nehereusmetabolomic Disclosure statementNo potential conflict of interest was reported by the author(s).Additional informationFundingThis study was funded by the Indonesian Endowment Fund for Education (LPDP) scholarship program for 2020. Our special thanks goes to Sri Iswani who assisted us with laboratory analysis. During the course of the study and its preparation for publication preparation, the author Giri Rohmad Barokah has been the main contributor.
甲醛可以通过酶促过程在水产品中内源性产生。然而,非法添加人造甲醛的做法在包括印度尼西亚在内的一些发展中国家仍然存在。本研究的目的是鉴定用于区分孟买鸭(Harpodon nehereus)作为样本渔业产品的内源性甲醛和人造甲醛的生物标志物。采用基于1H核磁共振的代谢组学方法鉴定样品中的代谢物。利用质子核磁共振对孟买鸭片样品进行代谢物分析,检测出11种代谢物(即乳酸、乙酸、氧化三甲胺(TMAO)、三甲胺(TMA)、二甲胺、赖氨酸、雁胺、肌苷、肌苷-5′-单磷酸肌苷和次黄嘌呤)。研究结果表明,该列表中的三种代谢物,即氧化三甲胺(TMAO)、三甲胺(TMA)和赖氨酸,可以作为区分孟买鸭鱼中内源甲醛和人工甲醛的指标。关键词:生物标志物甲醛鱼叉子代谢组学披露声明作者未报告潜在的利益冲突。本研究由2020年印尼教育捐赠基金(ldp)奖学金项目资助。我们特别感谢Sri Iswani,他协助我们进行了实验室分析。在研究和准备出版的过程中,作者Giri Rohmad Barokah一直是主要贡献者。
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引用次数: 0
Microbial Succession and Flavor Changes During Fermentation of Semi-Dried Sea Bass ( Lateolabrax maculatus ) 半干黑鲈发酵过程中微生物演替及风味变化
4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-09-20 DOI: 10.1080/10498850.2023.2258868
Jie Zheng, Hao Ding, Lirong Bai, Shuai Zhu, Hongyuan Hui, Xinyue Zhu, Wen Chen, Aijun Hu
ABSTRACTThe Chinese traditional food, fermented semi-dried sea bass (FSSB), is famous for its unique flavor. The microbiota succession and flavor formation during fermentation remain unknown. This study utilized high-throughput sequencing to investigate the evolving microbiota and chemical determination to explore volatile compounds. The relevant abundance of microorganisms in the middle-fermented stage was significantly (p <.05) higher than that of the post-fermented stage. In addition, the main microbial phyla during fermentation were Firmicutes and Proteobacteria. In conclusion, this study contributes to understanding the changes in flavor substances during fermentation and the varying patterns of change among different floras.KEYWORDS: Fermented fishsea bassflavor compoundshigh-throughput sequencing Disclosure statementNo potential conflict of interest was reported by the author(s).Additional informationFundingThis work was supported by the Tianjin Agriculture and Rural Committee [Contract no.: 201801170]; Tianjin Municipal Science and Technology Bureau [Contract no.: 17ZXYENC00140].
摘要发酵半干鲈鱼是中国传统食品,以其独特的风味而闻名。发酵过程中微生物群的继承和风味的形成尚不清楚。本研究利用高通量测序研究进化的微生物群和化学测定来探索挥发性化合物。发酵中期相关微生物丰度显著(p < 0.05)高于发酵后。发酵过程中主要的微生物门为厚壁菌门和变形菌门。综上所述,本研究有助于了解发酵过程中风味物质的变化以及不同菌群之间的变化规律。关键词:发酵鱼海鲈鱼风味化合物高通量测序披露声明作者未报告潜在利益冲突。本研究得到天津市农业和农村委员会的支持[合同编号:: 201801170);天津市科学技术局[合同编号:: 17 zxyenc00140]。
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引用次数: 0
Mapping of Marine Rest Raw Material in the Norwegian Seafood Industry: A Decade of Results 绘制海洋休息原料在挪威海产品产业:十年的成果
4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-09-10 DOI: 10.1080/10498850.2023.2255179
Magnus Stoud Myhre, Pernille Kristiane Skavang, Jannicke Remme, Robert Wolff, Ana Carvajal
From 2013 to 2022, the available volumes, degree of utilization, as well as the application of different fractions of marine rest raw materials, like heads and skins, originating from the Norwegian seafood industry have been studied and mapped. The majority of the rest raw materials were still used for feed applications in 2021, but there has been observed a steady increase in volume to direct or indirect human consumption products in the period. While there are efforts to produce new high-quality products, several barriers must be addressed before a significant increase in the valorization of the industry occurs.
从2013年到2022年,对来自挪威海产品行业的海洋休息原料(如鱼头和鱼皮)的可用量、利用程度以及不同馏分的应用进行了研究和绘制。2021年,其余大部分原材料仍用于饲料用途,但在此期间,直接或间接人类消费产品的数量稳步增长。虽然正在努力生产新的高质量产品,但在该行业的价值显著增加之前,必须解决几个障碍。
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引用次数: 3
Effect of Freezing Process on the Chemical Properties of Blue Swimming Crab Portunus segnis: Storage Stability and Acceptability 冷冻过程对青蟹化学性质的影响:贮藏稳定性和可接受性
IF 1.6 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-08-29 DOI: 10.1080/10498850.2023.2249477
F. Ghribi, S. Bejaoui, I. Chetoui, M. Marengo, Mehdi Bouaziz, N. Soudani, Sylvie Gobert, M. El Cafsi
ABSTRACT The effect of frozen storage at a commercial temperature (−30°C) on the nutritional characteristics of the blue swimming crab Portunus segnis for a period of 120 days was investigated. Lipid and protein oxidation was investigated by analyses of peroxide value, thiobarbituric acid reactive substances, free fatty acids, and advanced oxidation protein products. Results showed significant lipid deterioration with extended storage time and changes in fatty acids composition and lipid classes. From a nutritional standpoint, it would be preferable for human consumption to eat frozen crabs for no more than 30 days as they retain a higher nutritional value.
{"title":"Effect of Freezing Process on the Chemical Properties of Blue Swimming Crab Portunus segnis: Storage Stability and Acceptability","authors":"F. Ghribi, S. Bejaoui, I. Chetoui, M. Marengo, Mehdi Bouaziz, N. Soudani, Sylvie Gobert, M. El Cafsi","doi":"10.1080/10498850.2023.2249477","DOIUrl":"https://doi.org/10.1080/10498850.2023.2249477","url":null,"abstract":"ABSTRACT The effect of frozen storage at a commercial temperature (−30°C) on the nutritional characteristics of the blue swimming crab Portunus segnis for a period of 120 days was investigated. Lipid and protein oxidation was investigated by analyses of peroxide value, thiobarbituric acid reactive substances, free fatty acids, and advanced oxidation protein products. Results showed significant lipid deterioration with extended storage time and changes in fatty acids composition and lipid classes. From a nutritional standpoint, it would be preferable for human consumption to eat frozen crabs for no more than 30 days as they retain a higher nutritional value.","PeriodicalId":15091,"journal":{"name":"Journal of Aquatic Food Product Technology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42880888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of Biogenic Amine Degradation Ability of Staphylococcus xylosus JCM 2418 and its Application in Shrimp Paste 木糖葡萄球菌JCM 2418生物胺降解能力评价及其在虾膏中的应用
IF 1.6 4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-08-10 DOI: 10.1080/10498850.2023.2244948
Yang Zhang, Xinyu Li, Xinxiu Ma, Jingran Bi, Gongliang Zhang, H. Hou
ABSTRACT Microbial strains with amine oxidase activity could effectively degrade undesirable biogenic amines in food. Staphylococcus xylosus JCM 2418 was sensitive to common antibiotics, without hemolysis, and had protease activity. A 15% salt concentration was beneficial to the expression of the amine oxidase gene (yobN), restricted the expression of the lysine decarboxylase gene (ldc), and was more effective in degrading biogenic amines. The strain was inoculated in shrimp paste as a co-starter to promote the formation of volatile odor. Additionally, the tyramine, putrescine, cadaverine, and histamine levels were reduced by 42.4, 16.4, 22.2, and 20.8%, respectively.
{"title":"Evaluation of Biogenic Amine Degradation Ability of Staphylococcus xylosus JCM 2418 and its Application in Shrimp Paste","authors":"Yang Zhang, Xinyu Li, Xinxiu Ma, Jingran Bi, Gongliang Zhang, H. Hou","doi":"10.1080/10498850.2023.2244948","DOIUrl":"https://doi.org/10.1080/10498850.2023.2244948","url":null,"abstract":"ABSTRACT Microbial strains with amine oxidase activity could effectively degrade undesirable biogenic amines in food. Staphylococcus xylosus JCM 2418 was sensitive to common antibiotics, without hemolysis, and had protease activity. A 15% salt concentration was beneficial to the expression of the amine oxidase gene (yobN), restricted the expression of the lysine decarboxylase gene (ldc), and was more effective in degrading biogenic amines. The strain was inoculated in shrimp paste as a co-starter to promote the formation of volatile odor. Additionally, the tyramine, putrescine, cadaverine, and histamine levels were reduced by 42.4, 16.4, 22.2, and 20.8%, respectively.","PeriodicalId":15091,"journal":{"name":"Journal of Aquatic Food Product Technology","volume":null,"pages":null},"PeriodicalIF":1.6,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44527742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessment of Nutritional Constituents Content and Biomedical Aspects of Five Gracilaria Species: A Multivariate Analysis 五种葛属植物营养成分含量及生物医学评价:多变量分析
4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-08-09 DOI: 10.1080/10498850.2023.2256718
Dharmendra Kumar, Shivankar Agrawal, Manoj Kumar, Dinabandhu Sahoo
ABSTRACTThis study assesses the nutritional aspects of five Gracilaria sp. (G. verrucosa, G. corticata, G. crassa, G. cylindrical, and G. edulis) including their antioxidant and in-vitro anti-diabetic potential. Liquid chromatography-mass spectrometry (LC-MS) reveals the presence of free amino acids. Total phenolic content 13.25 ± 0.39 mg GAE g-1, flavonoid content 44.45 ± 0.36 mg GAE g-1, ferrous ion chelating ability 28.32%, and hydrogen peroxide radical scavenging activity 16.53 ± 0.5% contents were recorded highest in G. crassa, while DPPH activity was recorded highest in G. corticata 33.7 ± 0.35%, and total polyamine content was recorded maximum in G. edulis (2.54 ± 0.27). Polysaccharide yield in red alga Gracilaria sp. varies from highest (25.14 ± 0.6) in G. crassa to lowest (16.99 ± 0.53) in G. edulis. The highest number was 12 types of free amino acids present in G. edulis. The highest α‑amylase (46.80%) was recorded in G. crassa, while the highest α‑glucosidase (80.63%) was observed in G. corticata.KEYWORDS: Gracilaria sp.antioxidant activityα‑amylaseα‑glucosidasepolyaminesphenol compoundsmultivariate analysisprincipal component analysis Authors’ contributionsDK, SA, MK, and DS have a role in the design of the study, performed experiments, analysis, and interpretation of data, and all authors contributed to manuscript writing and approved the final manuscript.Disclosure statementNo potential conflict of interest was reported by the author(s).
摘要本研究评价了五种江蓠属植物(疣状江蓠、皮质江蓠、粗江蓠、圆柱江蓠和毛蕊江蓠)的抗氧化和体外抗糖尿病活性。液相色谱-质谱分析(LC-MS)显示游离氨基酸的存在。粗草中总酚含量最高(13.25±0.39 mg GAE g-1),类黄酮含量最高(44.45±0.36 mg GAE g-1),铁离子螯合能力最高(28.32%),过氧化氢自由基清除能力最高(16.53±0.5%),皮草中DPPH含量最高(33.7±0.35%),毛竹中总多胺含量最高(2.54±0.27)。红藻的多糖产量从粗草最高(25.14±0.6)到毛蕊最低(16.99±0.53)。毛竹中游离氨基酸最多,有12种。α -淀粉酶最高的是粗草(46.80%),α -葡萄糖苷酶最高的是皮草(80.63%)。作者的贡献(sdk、SA、MK和DS)参与了研究的设计、实验、分析和数据解释,所有作者都参与了论文的撰写并批准了最终稿件。披露声明作者未报告潜在的利益冲突。
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引用次数: 0
Inhibitory Mechanism of EGCG on Advanced Glycation End Products Formation During Low-Temperature Vacuum Heating of Russian Sturgeon EGCG对俄罗斯鲟鱼低温真空加热过程中晚期糖基化终产物形成的抑制机制
4区 农林科学 Q2 Agricultural and Biological Sciences Pub Date : 2023-08-09 DOI: 10.1080/10498850.2023.2257204
Yue-Wen Chen, Min Zhang, Jing-Jing Fu, Jian-Ling Wei, Wen-Qiang Cai, Xiu-Ping Dong
ABSTRACTAdvanced glycation end products (AGEs) are generated in all stages of food thermal treatment. In order to reveal the inhibitory mechanism of AGEs, a range of concentration of (−)-epigallocatechin-3-gallate (EGCG) has been added during low-temperature vacuum heating (LTVH), and high-resolution mass spectrometry-based proteomics was applied for identifying and quantifying N-glycosylated proteins, N-glycosylated peptides, and N-glycosylated sites. The results indicated that, compared with atmospheric cooking, LTVH significantly decreased the generation of AGEs. The addition of EGCG has a positive effect on the inhibition of AGEs when the concentration is lower than 2,000 μg/mL, and 200 μg/mL was the optimal concentration.KEYWORDS: Low-temperature vacuum heating (LTVH)advanced glycation end products(−)-epigallocatechin-3-gallate (EGCG)proteomics Disclosure statementNo potential conflict of interest was reported by the author(s).Ethical approvalEthics approval was not required for this research.Supplementary materialSupplemental data for this article can be accessed online at https://doi.org/10.1080/10498850.2023.2257204.Additional informationFundingThis work was supported by the Foundation of Zhejiang Educational Committee [Y201942135]; Zhejiang Provincial Natural Science Foundation [LQ21C200004]; National Key R&D Program of China [2018YFD0400600].
在食品热处理的各个阶段都会产生晚期糖基化终产物。为了揭示AGEs的抑制机制,在低温真空加热(LTVH)过程中加入一定浓度的(−)-表没食子儿茶素-3-没食子酸酯(EGCG),并应用基于高分辨率质谱的蛋白质组学技术鉴定和定量n-糖基化蛋白、n-糖基化肽和n-糖基化位点。结果表明,与常压蒸煮相比,低温蒸煮显著降低了AGEs的生成。当EGCG浓度低于2000 μg/mL时,EGCG对AGEs的抑制有正向作用,200 μg/mL为最佳浓度。关键词:低温真空加热(LTVH)晚期糖基化终产物(−)-表没食子儿茶素-3-没食子酸酯(EGCG)蛋白质组学披露声明作者未报告潜在的利益冲突。伦理批准本研究不需要伦理批准。补充资料本文补充资料可在线获取:https://doi.org/10.1080/10498850.2023.2257204.Additional information经费来源:浙江省教委基金资助[Y201942135];浙江省自然科学基金[LQ21C200004];国家重点研发计划[2018YFD0400600]。
{"title":"Inhibitory Mechanism of EGCG on Advanced Glycation End Products Formation During Low-Temperature Vacuum Heating of Russian Sturgeon","authors":"Yue-Wen Chen, Min Zhang, Jing-Jing Fu, Jian-Ling Wei, Wen-Qiang Cai, Xiu-Ping Dong","doi":"10.1080/10498850.2023.2257204","DOIUrl":"https://doi.org/10.1080/10498850.2023.2257204","url":null,"abstract":"ABSTRACTAdvanced glycation end products (AGEs) are generated in all stages of food thermal treatment. In order to reveal the inhibitory mechanism of AGEs, a range of concentration of (−)-epigallocatechin-3-gallate (EGCG) has been added during low-temperature vacuum heating (LTVH), and high-resolution mass spectrometry-based proteomics was applied for identifying and quantifying N-glycosylated proteins, N-glycosylated peptides, and N-glycosylated sites. The results indicated that, compared with atmospheric cooking, LTVH significantly decreased the generation of AGEs. The addition of EGCG has a positive effect on the inhibition of AGEs when the concentration is lower than 2,000 μg/mL, and 200 μg/mL was the optimal concentration.KEYWORDS: Low-temperature vacuum heating (LTVH)advanced glycation end products(−)-epigallocatechin-3-gallate (EGCG)proteomics Disclosure statementNo potential conflict of interest was reported by the author(s).Ethical approvalEthics approval was not required for this research.Supplementary materialSupplemental data for this article can be accessed online at https://doi.org/10.1080/10498850.2023.2257204.Additional informationFundingThis work was supported by the Foundation of Zhejiang Educational Committee [Y201942135]; Zhejiang Provincial Natural Science Foundation [LQ21C200004]; National Key R&D Program of China [2018YFD0400600].","PeriodicalId":15091,"journal":{"name":"Journal of Aquatic Food Product Technology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135746758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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Journal of Aquatic Food Product Technology
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