Pub Date : 2023-01-01DOI: 10.5958/2277-8934.2023.00025.5
M. Hossein, Young-Bum Son, Y. Jeong, Mina Kang, Hui-Choun Kim, Yu-Chan Bae, Han Seock Kim, Ji-Young Noh, Kyung Ik Hwang, W. Hwang
Evaluation of the dose-specific effects of ionomycin on the parthenogenetic activation of Camelus dromedarius oocytes was done in the present study. Ovaries were collected from a local slaughterhouse. Cumulus-oocyte-complexes (COCs) were aspirated and cultured in a commercial IVM medium (BO-IVM) for 42 hrs in a humidified atmosphere containing 5% CO 2 at 38°C. All metaphase II oocytes were activated either 2.5 µM, 5.0 µM or 10.0 µM of ionomycin for 4 min. Activated oocytes were immediately incubated in 2.0 mM 6-dimethylaminopurine (6-DMAP) in a commercial embryo culture medium (BO-IVC) for 4 hrs at 39°C in a humidified incubator with 5% CO 2 . Oocytes were cultured in BO-IVC for 7 days and developmental stages were monitored and recorded. After 42 hrs of in vitro maturation, 71.28% of oocytes were found with the extruded first polar body (metaphase II oocytes). The oocytes in the 5.0 µM of ionomycin group showed the highest blastocyst formation rate (56.99%) compared to the 2.5 µM (6.58%) and 10.0 µM (1.81%) groups. We recommended 5.0 µM of ionomycin for 4 min followed by incubation in 2.0 mM 6-DMAP for 4 hrs to activate camel oocytes.
本研究评价了离子霉素对骆驼卵母细胞孤雌生殖激活的剂量特异性效应。卵巢是从当地一家屠宰场采集的。将积云卵母细胞复合物(COCs)在商业IVM培养基(BO-IVM)中抽吸并在含5% co2的38°C湿化气氛中培养42小时。所有中期II卵母细胞分别被2.5µM、5.0µM或10.0µM的离子霉素激活4分钟。被激活的卵母细胞立即在2.0 mM 6-二甲氨基嘌呤(6-DMAP)中，在商业胚胎培养基(BO-IVC)中，在含5%二氧化碳的39°C加湿培养箱中孵育4小时。卵母细胞在BO-IVC中培养7 d，监测并记录发育阶段。体外成熟42 h后，71.28%的卵母细胞出现挤压的第一极体(中期卵母细胞)。与2.5µM组(6.58%)和10.0µM组(1.81%)相比，5.0µM组的卵母细胞囊胚形成率最高(56.99%)。我们推荐用5.0µM离子霉素孵育4分钟，然后用2.0 mM 6-DMAP孵育4小时来激活骆驼卵母细胞。
{"title":"Dose Specific Effects of Ionomycin on Parthenogenetic Activation of in vitro Matured Dromedary Oocytes","authors":"M. Hossein, Young-Bum Son, Y. Jeong, Mina Kang, Hui-Choun Kim, Yu-Chan Bae, Han Seock Kim, Ji-Young Noh, Kyung Ik Hwang, W. Hwang","doi":"10.5958/2277-8934.2023.00025.5","DOIUrl":"https://doi.org/10.5958/2277-8934.2023.00025.5","url":null,"abstract":"Evaluation of the dose-specific effects of ionomycin on the parthenogenetic activation of Camelus dromedarius oocytes was done in the present study. Ovaries were collected from a local slaughterhouse. Cumulus-oocyte-complexes (COCs) were aspirated and cultured in a commercial IVM medium (BO-IVM) for 42 hrs in a humidified atmosphere containing 5% CO 2 at 38°C. All metaphase II oocytes were activated either 2.5 µM, 5.0 µM or 10.0 µM of ionomycin for 4 min. Activated oocytes were immediately incubated in 2.0 mM 6-dimethylaminopurine (6-DMAP) in a commercial embryo culture medium (BO-IVC) for 4 hrs at 39°C in a humidified incubator with 5% CO 2 . Oocytes were cultured in BO-IVC for 7 days and developmental stages were monitored and recorded. After 42 hrs of in vitro maturation, 71.28% of oocytes were found with the extruded first polar body (metaphase II oocytes). The oocytes in the 5.0 µM of ionomycin group showed the highest blastocyst formation rate (56.99%) compared to the 2.5 µM (6.58%) and 10.0 µM (1.81%) groups. We recommended 5.0 µM of ionomycin for 4 min followed by incubation in 2.0 mM 6-DMAP for 4 hrs to activate camel oocytes.","PeriodicalId":15097,"journal":{"name":"Journal of Camel Practice and Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71074108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5958/2277-8934.2023.00028.0
Sanwar Mal, S. Joshi, P. Thanvi, Devendra Singh, Vijay Yogi, Priyanka, Pura Ram, Rajesh Kumar Siyag
The investigation was carried out on the adrenal glands of 6 recently dead adult camels. Histologically, the adrenals were divided into stroma and parenchyma. Stroma consisted of capsule and trabeculae. Collagen and reticular fibres were present at the capsule and trabeculae. Parenchyma was composed of the cortex and medulla. The cortex was divided into three parts according to their cell arrangement: zona glomerulosa, zona fasciculata, and zona reticularis. The trabeculae entered into the cortex at various distances. The medulla was divided into the inner and outer parts. The outer zone was lined by columnar-shaped cells, and the inner area had polyhedral cells. Patches of the medulla were seen in some cortical areas. The adrenal gland of the camel was surrounded by a thick layer of dense connective tissue fibres, specially collagen and reticular predominance over the elastic and muscle fibres.
{"title":"Histological Study of Adrenal Gland of One Humped Camel (Camelus dromedarius)","authors":"Sanwar Mal, S. Joshi, P. Thanvi, Devendra Singh, Vijay Yogi, Priyanka, Pura Ram, Rajesh Kumar Siyag","doi":"10.5958/2277-8934.2023.00028.0","DOIUrl":"https://doi.org/10.5958/2277-8934.2023.00028.0","url":null,"abstract":"The investigation was carried out on the adrenal glands of 6 recently dead adult camels. Histologically, the adrenals were divided into stroma and parenchyma. Stroma consisted of capsule and trabeculae. Collagen and reticular fibres were present at the capsule and trabeculae. Parenchyma was composed of the cortex and medulla. The cortex was divided into three parts according to their cell arrangement: zona glomerulosa, zona fasciculata, and zona reticularis. The trabeculae entered into the cortex at various distances. The medulla was divided into the inner and outer parts. The outer zone was lined by columnar-shaped cells, and the inner area had polyhedral cells. Patches of the medulla were seen in some cortical areas. The adrenal gland of the camel was surrounded by a thick layer of dense connective tissue fibres, specially collagen and reticular predominance over the elastic and muscle fibres.","PeriodicalId":15097,"journal":{"name":"Journal of Camel Practice and Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71074179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5958/2277-8934.2023.00029.2
S. Arroum, A. Sboui, M. Atigui, I. Fguiri, M. Dbara, N. Ayeb, Zahra Farah, M. Hammadi, T. Khorchani
{"title":"Camel’s Kefir Milk: Optimisation of Processing Conditions","authors":"S. Arroum, A. Sboui, M. Atigui, I. Fguiri, M. Dbara, N. Ayeb, Zahra Farah, M. Hammadi, T. Khorchani","doi":"10.5958/2277-8934.2023.00029.2","DOIUrl":"https://doi.org/10.5958/2277-8934.2023.00029.2","url":null,"abstract":"","PeriodicalId":15097,"journal":{"name":"Journal of Camel Practice and Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71074192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5958/2277-8934.2023.00016.4
A. Sara, M. D. Tingari, H. Ismail
{"title":"Ultrastructural and morphometric studies on the pelvic urethral gland of the one-humped camel (Camelus dromedarius)","authors":"A. Sara, M. D. Tingari, H. Ismail","doi":"10.5958/2277-8934.2023.00016.4","DOIUrl":"https://doi.org/10.5958/2277-8934.2023.00016.4","url":null,"abstract":"","PeriodicalId":15097,"journal":{"name":"Journal of Camel Practice and Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71074367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5958/2277-8934.2023.00023.1
T. Munkhjargal, B. Chinchuluun, S. Lkhagvatseren, S. Gantuya, G. Battsetseg, Z. Batsukh, R. Schuster, U. Wernery
{"title":"Review of Bacterial and Viral Infections of Bactrian Camels in Mongolia","authors":"T. Munkhjargal, B. Chinchuluun, S. Lkhagvatseren, S. Gantuya, G. Battsetseg, Z. Batsukh, R. Schuster, U. Wernery","doi":"10.5958/2277-8934.2023.00023.1","DOIUrl":"https://doi.org/10.5958/2277-8934.2023.00023.1","url":null,"abstract":"","PeriodicalId":15097,"journal":{"name":"Journal of Camel Practice and Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71074486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5958/2277-8934.2023.00021.8
M. Lal, Neelam Mahala, Aastha Mittal, Anisha Ramdas, Chetan Shrivastava, Uma S. Dubey
{"title":"Studies on Cytotoxic Activity of Camel Milk Whey Protein as a Nutraceutical against HeLa Cells","authors":"M. Lal, Neelam Mahala, Aastha Mittal, Anisha Ramdas, Chetan Shrivastava, Uma S. Dubey","doi":"10.5958/2277-8934.2023.00021.8","DOIUrl":"https://doi.org/10.5958/2277-8934.2023.00021.8","url":null,"abstract":"","PeriodicalId":15097,"journal":{"name":"Journal of Camel Practice and Research","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71074568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5958/2277-8934.2023.00002.4
Alsaleem Ahmed, Kandeel Mahmoud
{"title":"MVA-based vaccine efficacies on the immune response and seroprevalence of MERS-CoV: A systematic review","authors":"Alsaleem Ahmed, Kandeel Mahmoud","doi":"10.5958/2277-8934.2023.00002.4","DOIUrl":"https://doi.org/10.5958/2277-8934.2023.00002.4","url":null,"abstract":"","PeriodicalId":15097,"journal":{"name":"Journal of Camel Practice and Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71073805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5958/2277-8934.2023.00007.3
Neelam Mahala, Aastha Mittal, M. Lal, Uma S. Dubey
{"title":"Chromatographic purification of proteins with cytotoxic potential from camel milk whey against cervical cancer cell line","authors":"Neelam Mahala, Aastha Mittal, M. Lal, Uma S. Dubey","doi":"10.5958/2277-8934.2023.00007.3","DOIUrl":"https://doi.org/10.5958/2277-8934.2023.00007.3","url":null,"abstract":"","PeriodicalId":15097,"journal":{"name":"Journal of Camel Practice and Research","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71073939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5958/2277-8934.2023.00015.2
S. Vyas, R. K. Sawal, Mohammed Matin Ansari, K. Nath, Subroto Vyas, G. Purohit
{"title":"Ultrasonographic assessment of follicular size to reduce the age of first service in the dromedary heifers","authors":"S. Vyas, R. K. Sawal, Mohammed Matin Ansari, K. Nath, Subroto Vyas, G. Purohit","doi":"10.5958/2277-8934.2023.00015.2","DOIUrl":"https://doi.org/10.5958/2277-8934.2023.00015.2","url":null,"abstract":"","PeriodicalId":15097,"journal":{"name":"Journal of Camel Practice and Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71074336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.5958/2277-8934.2023.00024.3
M. Waheed, I. Ghoneim, S. El-Bahr
{"title":"Analysis of Glycosidase Activities, Steroids Hormones Concentrations and Selected Biochemical Parameters in Serum of Dromedary Camels (Camelus dromedarius) during Follicular Phase and Parturition","authors":"M. Waheed, I. Ghoneim, S. El-Bahr","doi":"10.5958/2277-8934.2023.00024.3","DOIUrl":"https://doi.org/10.5958/2277-8934.2023.00024.3","url":null,"abstract":"","PeriodicalId":15097,"journal":{"name":"Journal of Camel Practice and Research","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71074500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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