Pub Date : 2023-05-27DOI: 10.20473/jafh.v12i2.37044
Giri Pratama, A. Prajitno, T. D. Sulistiyati, Desy Amalia Hidayati, Tania Nilakhandi
This study aimed to determine the antibacterial activity of bitter leaf extract (A. paniculata) against the growth of E. tarda bacteria. This test was carried out using Kirby Bauer method (disc test) using five different concentrations, namely 50 mg/L, 100 mg/L, 150 mg/L, 200 mg/L, and 250 mg/L in TSA (Tryptone Soya Agar) and incubated for 2x24 hours using a temperature of 30 oC. The positive control used was chloramphenicol (5 mg/L), while the negative control used PBS. Bitter leaf extract contains active compounds that have bacteriostatic antibacterial properties. After incubation for 24 hours the concentration of 250 mg/L showed the highest inhibition zone with a diameter of 7.82 mm and decreased at 48 hours to 7.46 mm. After being incubated for 24 hours, the concentration of 250 mg/L obtained an effectiveness value of 57.08% which is the best effectiveness, and there was a decrease in the 48th hour to 54.45%.
{"title":"Effect of Bitter Leaf Extract (Andrographis paniculata) against Edwardsiella tarda Bacteria In vitro","authors":"Giri Pratama, A. Prajitno, T. D. Sulistiyati, Desy Amalia Hidayati, Tania Nilakhandi","doi":"10.20473/jafh.v12i2.37044","DOIUrl":"https://doi.org/10.20473/jafh.v12i2.37044","url":null,"abstract":"This study aimed to determine the antibacterial activity of bitter leaf extract (A. paniculata) against the growth of E. tarda bacteria. This test was carried out using Kirby Bauer method (disc test) using five different concentrations, namely 50 mg/L, 100 mg/L, 150 mg/L, 200 mg/L, and 250 mg/L in TSA (Tryptone Soya Agar) and incubated for 2x24 hours using a temperature of 30 oC. The positive control used was chloramphenicol (5 mg/L), while the negative control used PBS. Bitter leaf extract contains active compounds that have bacteriostatic antibacterial properties. After incubation for 24 hours the concentration of 250 mg/L showed the highest inhibition zone with a diameter of 7.82 mm and decreased at 48 hours to 7.46 mm. After being incubated for 24 hours, the concentration of 250 mg/L obtained an effectiveness value of 57.08% which is the best effectiveness, and there was a decrease in the 48th hour to 54.45%.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43968631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-27DOI: 10.20473/jafh.v12i2.37049
Arung Mulya, Sania Citra, Ari Okta Andini, Rasali Abdur rahman, Win Ariga, S. Cahyani, Izzul Islam, V. Hasan
Marente is a village at the foot of the ancient Sumbawa volcano, located on the Tiu Kele river and the Agal waterfall, which empties directly into the sea. The ecological condition of the Tiu Kele river is an ideal environment for Stiphodon fish to live and breed. Therefore, this study focused on identifying the species of Stiphodon sp. (Gobiidae), whose presence has not been recorded in West Nusa Tenggara. There is still no literature study related to the Gobiidae family, especially the genus Stiphodon in the waters of the Tiu Kele river, which makes this research very important to do. This research was conducted from September 13 to October 13, 2021, in Tiu Kele river, Marente village, Sumbawa. The tools used are net, dipper, aquarium, sampling tubes, thermometer, pH meter, and camera, while the material used is 96% alcohol for specimen preparation. The identification results stated that Stiphodon atropurpureus had clearly visible characteristics. It had a bright blue body from the snout and cheeks to the tail, the dorsal fin is separated and fibrous. These results were also later strengthened from various related references about the physical properties and characteristics of Stiphodon atropurpureus. The results of this research can later be used as a reference and new data related to the distribution of stiphodon in Indonesia, especially in Lesser Sunda.
{"title":"Fist Report of Stiphodon atropurpureus (Herre, 1927) in Marente Village, Sumbawa, West Nusa Tenggara","authors":"Arung Mulya, Sania Citra, Ari Okta Andini, Rasali Abdur rahman, Win Ariga, S. Cahyani, Izzul Islam, V. Hasan","doi":"10.20473/jafh.v12i2.37049","DOIUrl":"https://doi.org/10.20473/jafh.v12i2.37049","url":null,"abstract":"Marente is a village at the foot of the ancient Sumbawa volcano, located on the Tiu Kele river and the Agal waterfall, which empties directly into the sea. The ecological condition of the Tiu Kele river is an ideal environment for Stiphodon fish to live and breed. Therefore, this study focused on identifying the species of Stiphodon sp. (Gobiidae), whose presence has not been recorded in West Nusa Tenggara. There is still no literature study related to the Gobiidae family, especially the genus Stiphodon in the waters of the Tiu Kele river, which makes this research very important to do. This research was conducted from September 13 to October 13, 2021, in Tiu Kele river, Marente village, Sumbawa. The tools used are net, dipper, aquarium, sampling tubes, thermometer, pH meter, and camera, while the material used is 96% alcohol for specimen preparation. The identification results stated that Stiphodon atropurpureus had clearly visible characteristics. It had a bright blue body from the snout and cheeks to the tail, the dorsal fin is separated and fibrous. These results were also later strengthened from various related references about the physical properties and characteristics of Stiphodon atropurpureus. The results of this research can later be used as a reference and new data related to the distribution of stiphodon in Indonesia, especially in Lesser Sunda.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43560158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-27DOI: 10.20473/jafh.v12i2.22985
Widyaningsih Rahayu, Dahlia Wulan Sari
As population activity is increasing and the Kapuas river is still a means of transportation, causing Corbicula largillierti to accumulate heavy metals (Pb) and Coliform bacteria in their bodies. This makes it dangerous to be consumed by humans. Thus, this research has two main aims. First, to find out the concentration of Pb and Coliform inside Corbicula largillierti that lives at Kapuas river, Pontianak and the river’s water. Second, to analyze the health risk of consuming the clams. Furthermore, the Atomic Absorption Spectrophotometer (AAS) is used to analyze the Pb sample and the Most Probable Number (MPN) method is used to analyze the Coliform. The result of this research shows that concentration Pb and Coliform from the Kapuas river have exceeded the limits designated for aquatic biota based on the Decree of the State Minister for the Environment No. 51 of 2004. In addition, the health risk is measured using Estimated Daily Intake (EDI), Target Hazard Quotient (THQ) and Target Carcinogenic Risk (TR) with scores for each are >10 times the RfD, >1 and 10−3. This result shows that based on the clam Corbicula largillierti consumption for 70 years life span of 60 kg body weight, had a high and negative effect on health risk problems and had a cancer risk for consumers. However, the health risk problem might increase much worse due to the long and continual period of consumption exceeding estimated daily intake.
{"title":"Health Risk Assessment Via the Consumption of Clam (Corbicula largillierti) Accumulated Heavy Metals (Pb) and Coliform Bacteria at Kapuas River, West Borneo","authors":"Widyaningsih Rahayu, Dahlia Wulan Sari","doi":"10.20473/jafh.v12i2.22985","DOIUrl":"https://doi.org/10.20473/jafh.v12i2.22985","url":null,"abstract":"As population activity is increasing and the Kapuas river is still a means of transportation, causing Corbicula largillierti to accumulate heavy metals (Pb) and Coliform bacteria in their bodies. This makes it dangerous to be consumed by humans. Thus, this research has two main aims. First, to find out the concentration of Pb and Coliform inside Corbicula largillierti that lives at Kapuas river, Pontianak and the river’s water. Second, to analyze the health risk of consuming the clams. Furthermore, the Atomic Absorption Spectrophotometer (AAS) is used to analyze the Pb sample and the Most Probable Number (MPN) method is used to analyze the Coliform. The result of this research shows that concentration Pb and Coliform from the Kapuas river have exceeded the limits designated for aquatic biota based on the Decree of the State Minister for the Environment No. 51 of 2004. In addition, the health risk is measured using Estimated Daily Intake (EDI), Target Hazard Quotient (THQ) and Target Carcinogenic Risk (TR) with scores for each are >10 times the RfD, >1 and 10−3. This result shows that based on the clam Corbicula largillierti consumption for 70 years life span of 60 kg body weight, had a high and negative effect on health risk problems and had a cancer risk for consumers. However, the health risk problem might increase much worse due to the long and continual period of consumption exceeding estimated daily intake.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43166539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-27DOI: 10.20473/jafh.v12i2.35023
K. Rahardjo, Woro Hastuti Setyantini, M. Amin
Shrimp farmers have reported mass mortality of white shrimp (Litopenaeus vannamei), reaching 2-3 kg/day in Gerongan Village, Kraton District, Pasuruan Regency in mid-2021. Preliminary analysis suggests that mass mortality was caused by bacterial diseases. Thus, to find out the main pathogen causing the mass mortality, the present study investigated microbial composition in rearing media of white shrimp (Litopenaeus vannamei) infected by the white feces disease (WFD) and healthy white shrimp using next-generation sequencing (NGS) technology. The research was conducted by collecting normal water samples and infected shrimp pond water samples. The results of the NGS assay showed that Vibrio vulnificus was found dominant in WFD-infected shrimp pond water, therefore was suspected to be the main cause of WFD.
{"title":"Profiling of Microbial Community in Rearing Water of White Shrimp (Litopenaeus vannamei) Infected with White Feces Disease Syndrome","authors":"K. Rahardjo, Woro Hastuti Setyantini, M. Amin","doi":"10.20473/jafh.v12i2.35023","DOIUrl":"https://doi.org/10.20473/jafh.v12i2.35023","url":null,"abstract":"Shrimp farmers have reported mass mortality of white shrimp (Litopenaeus vannamei), reaching 2-3 kg/day in Gerongan Village, Kraton District, Pasuruan Regency in mid-2021. Preliminary analysis suggests that mass mortality was caused by bacterial diseases. Thus, to find out the main pathogen causing the mass mortality, the present study investigated microbial composition in rearing media of white shrimp (Litopenaeus vannamei) infected by the white feces disease (WFD) and healthy white shrimp using next-generation sequencing (NGS) technology. The research was conducted by collecting normal water samples and infected shrimp pond water samples. The results of the NGS assay showed that Vibrio vulnificus was found dominant in WFD-infected shrimp pond water, therefore was suspected to be the main cause of WFD.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49522922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-27DOI: 10.20473/jafh.v12i2.32371
H. Ariadi, Tholibah Mujtahidah, Abdul Wafi
The purpose of this study was to evaluate the suitability of water quality parameters in intensive white shrimp (Litopenaeus vannamei) cultivation activities based on Good Aquaculture Practice (GAP) quality standards implication practice. This research was carried out with the ex-pose facto causal design concept during one cycle of shrimp cultivation, with the indicator being studied was the condition of the water quality parameters which were then corrected with the GAP standard according to the Minister of Agriculture Regulation No. 75 of 2016. The results showed that the condition of the water quality parameters during the cultivation period was still in accordance with the GAP quality standards, except for the alkalinity parameter which had a value of 157 mg/L and organic matter 104.43 mg/L, both values were above the GAP quality standard threshold. The abnormal condition of the two parameters was caused by unpredictable natural and seasonal factors. This can be seen from the trend of the temperature and salinity parameter graphs that fluctuate unstable. Furthermore, for technical parameters, the cultivation system at the research site was still in accordance with the cultivation quality standards listed in the GAP. Based on the discussions, it can be concluded that the water quality parameters at the research pond location as a whole were still in accordance with the GAP quality standard which refers to the Minister of Fisheries Regulation No. 75 of 2016, except for the alkalinity and organic matter parameters which had a slightly worse concentration than the GAP quality standard.
{"title":"Implications of Good Aquaculture Practice (GAP) Application on Intensive Shrimp Ponds and The Effect on Water Quality Parameter Compatibility","authors":"H. Ariadi, Tholibah Mujtahidah, Abdul Wafi","doi":"10.20473/jafh.v12i2.32371","DOIUrl":"https://doi.org/10.20473/jafh.v12i2.32371","url":null,"abstract":"The purpose of this study was to evaluate the suitability of water quality parameters in intensive white shrimp (Litopenaeus vannamei) cultivation activities based on Good Aquaculture Practice (GAP) quality standards implication practice. This research was carried out with the ex-pose facto causal design concept during one cycle of shrimp cultivation, with the indicator being studied was the condition of the water quality parameters which were then corrected with the GAP standard according to the Minister of Agriculture Regulation No. 75 of 2016. The results showed that the condition of the water quality parameters during the cultivation period was still in accordance with the GAP quality standards, except for the alkalinity parameter which had a value of 157 mg/L and organic matter 104.43 mg/L, both values were above the GAP quality standard threshold. The abnormal condition of the two parameters was caused by unpredictable natural and seasonal factors. This can be seen from the trend of the temperature and salinity parameter graphs that fluctuate unstable. Furthermore, for technical parameters, the cultivation system at the research site was still in accordance with the cultivation quality standards listed in the GAP. Based on the discussions, it can be concluded that the water quality parameters at the research pond location as a whole were still in accordance with the GAP quality standard which refers to the Minister of Fisheries Regulation No. 75 of 2016, except for the alkalinity and organic matter parameters which had a slightly worse concentration than the GAP quality standard.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46397565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-27DOI: 10.20473/jafh.v12i2.36747
Eka Fitria Febriani, M. Widodo, A. Faqih
Red ginger (Zingiber officinale var. Rubrum) as one of the Indonesian spices has so many important roles, especially in the health sector as a medicinal plant, which has many active compounds including phenols, alkaloids, flavonoids, steroids and tannins. From some researches before, we can know that plants which grow in different places or locations have different tolerances generally. That statements can show that they have different content in their constituent metabolites definitely. Therefore, phytochemical analysis is needed to analyze the content of each red ginger (Z. officinale var. Rubrum) from three locations such as Red ginger (Z. officinale var. Rubrum) from Batu, Malang (RGB), Plaosan, Magetan (RGP) and Simalungun, Medan (RGS) to analyze each content its contains. Also, perform LC50 was needed for analyzing its effect on Zebra fish (Danio rerio) as a model fish. For this research, we use an experimental study with a Completely Randomized Factorial Design (CRFD) method that has two independent variable such as different dosage and type of red ginger (Z. officinale var. Rubrum). In this study, from five dosages (10 mg/l; 20 mg/l; 30 mg/l; 40 mg/l; 50 mg/l) and three types of red ginger (Z. officinale var. Rubrum) treatment, it found that the highest mortality and lowest survival rate were at the highest dosage (50 mg/l) in RGB and extreme increase or decrease in the curve is found in RGP treatment.
红姜(Zingiber officinale var. Rubrum)作为印度尼西亚香料之一，具有许多重要的作用，特别是在卫生部门作为药用植物，它具有许多活性化合物，包括酚类，生物碱，类黄酮，类固醇和单宁。从之前的一些研究中，我们可以知道，生长在不同地方或地点的植物通常具有不同的耐受性。这些陈述可以明确地表明它们的组成代谢物含量不同。因此，需要进行植物化学分析，对巴都马郎红姜(RGB)、老山马吉丹红姜(RGP)和棉兰司马伦贡红姜(RGS)三个产地的每种红姜(Z. officinale var. Rubrum)的含量进行分析，以分析其所含的每种成分。此外，还需要进行LC50来分析其对斑马鱼(Danio rerio)作为模型鱼的影响。本研究采用完全随机因子设计(CRFD)方法进行实验研究，该方法有两个自变量，即不同剂量和不同类型的红姜(Z. officinale var. Rubrum)。在本研究中，从五种剂量(10mg /l;20 mg / l;30毫克/升;40 mg / l;50 mg/l)和3种红姜(Z. officinale var. Rubrum)处理后，发现RGB在最高剂量(50 mg/l)时死亡率最高，存活率最低，RGP处理时曲线急剧上升或下降。
{"title":"Phytochemical Analysis from Three Different Methanolic Extracts of Red Ginger (Zingiber officinale var. Rubrum) Against LC50 Treatment of Zebra Fish as Model Fish","authors":"Eka Fitria Febriani, M. Widodo, A. Faqih","doi":"10.20473/jafh.v12i2.36747","DOIUrl":"https://doi.org/10.20473/jafh.v12i2.36747","url":null,"abstract":"Red ginger (Zingiber officinale var. Rubrum) as one of the Indonesian spices has so many important roles, especially in the health sector as a medicinal plant, which has many active compounds including phenols, alkaloids, flavonoids, steroids and tannins. From some researches before, we can know that plants which grow in different places or locations have different tolerances generally. That statements can show that they have different content in their constituent metabolites definitely. Therefore, phytochemical analysis is needed to analyze the content of each red ginger (Z. officinale var. Rubrum) from three locations such as Red ginger (Z. officinale var. Rubrum) from Batu, Malang (RGB), Plaosan, Magetan (RGP) and Simalungun, Medan (RGS) to analyze each content its contains. Also, perform LC50 was needed for analyzing its effect on Zebra fish (Danio rerio) as a model fish. For this research, we use an experimental study with a Completely Randomized Factorial Design (CRFD) method that has two independent variable such as different dosage and type of red ginger (Z. officinale var. Rubrum). In this study, from five dosages (10 mg/l; 20 mg/l; 30 mg/l; 40 mg/l; 50 mg/l) and three types of red ginger (Z. officinale var. Rubrum) treatment, it found that the highest mortality and lowest survival rate were at the highest dosage (50 mg/l) in RGB and extreme increase or decrease in the curve is found in RGP treatment.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46138033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-18DOI: 10.20473/jafh.v12i1.36940
Tania Nilakandhi, A. Prajitno, Mohammad Fadjar, Desy Amalia Hidayati, Giri Pratama
Barriers to cultivation activities are disease attacks, one of which is caused by infection with the bacterium Edwardsiella tarda. Treatment efforts can be made using natural ingredients such as jackfruit (A. heterophyllus) leaves. This study aims to determine the content of antibacterial compounds in the jackfruit (A. heterophyllus) leaves crude extract and their effect on Edwardsiella tarda bacteria in vitro. The inhibition test used disc test with five different concentrations of jackfruit (A. heterophyllus) leaves crude extract with three replications: 75 mg/L, 150 mg/L, 225 mg/L, 300 mg/L and 375 mg/L with a comparison using two types of controls (positive with 5 mg/L chloramphenicol antibiotics and negative with no treatment) using Tryptone Soy Agar (TSA) media and incubated for 2x24 hours. The results of phytochemical screening proved that the jackfruit (A. heterophyllus) leaves crude extract contains flavonoids, alkaloids, tannins and saponins. Treatment E with a dose of 375 mg/L gave the highest inhibition of 7.80 mm and treatment A with a dose of 75 mg/L gave the lowest inhibition of 4.38 mm. The relationship between the treatment given to the test parameter in the form of the resulting inhibitory power obtained the equation y = 0.0111x + 3.6297 with an R2 value of 0.974 which means 97% of the treatment given in the form of crude extract of jackfruit (A. heterophyllus) leaves affects inhibition of bacteria E. tarda.
{"title":"Effect of Giving Jackfruit (Artocarpus heterophyllus) Leaves Crude Extract as An Alternative Antibacterial of Edwardsiella tarda Bacteria In Vitro","authors":"Tania Nilakandhi, A. Prajitno, Mohammad Fadjar, Desy Amalia Hidayati, Giri Pratama","doi":"10.20473/jafh.v12i1.36940","DOIUrl":"https://doi.org/10.20473/jafh.v12i1.36940","url":null,"abstract":"Barriers to cultivation activities are disease attacks, one of which is caused by infection with the bacterium Edwardsiella tarda. Treatment efforts can be made using natural ingredients such as jackfruit (A. heterophyllus) leaves. This study aims to determine the content of antibacterial compounds in the jackfruit (A. heterophyllus) leaves crude extract and their effect on Edwardsiella tarda bacteria in vitro. The inhibition test used disc test with five different concentrations of jackfruit (A. heterophyllus) leaves crude extract with three replications: 75 mg/L, 150 mg/L, 225 mg/L, 300 mg/L and 375 mg/L with a comparison using two types of controls (positive with 5 mg/L chloramphenicol antibiotics and negative with no treatment) using Tryptone Soy Agar (TSA) media and incubated for 2x24 hours. The results of phytochemical screening proved that the jackfruit (A. heterophyllus) leaves crude extract contains flavonoids, alkaloids, tannins and saponins. Treatment E with a dose of 375 mg/L gave the highest inhibition of 7.80 mm and treatment A with a dose of 75 mg/L gave the lowest inhibition of 4.38 mm. The relationship between the treatment given to the test parameter in the form of the resulting inhibitory power obtained the equation y = 0.0111x + 3.6297 with an R2 value of 0.974 which means 97% of the treatment given in the form of crude extract of jackfruit (A. heterophyllus) leaves affects inhibition of bacteria E. tarda.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45779460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-18DOI: 10.20473/jafh.v12i1.36844
Diana Permatasari, Mohamad Fadjar, Ating Yuniarti
Aquaculture has a land potential of 17.92 million ha consisting of 2.83 million ha of freshwater aquaculture potential, 2.96 million ha of brackish water aquaculture and 12.12 million ha of marine aquaculture. One of the freshwater species that has the potential to be cultivated is catfish (C. gariepinus). But now, as a result of the decline in the quality of the freshwater environment, catfish are prone to contamination by plastic waste. If microplastic particles have accumulated in large numbers in the fish's body, it can clog the fish's digestive tract and affect the growth of catfish which will affect the results of aquaculture production. The purpose of this study was to determine the survival rate or survival rate of catfish exposed to microplastics. The research design used in this study was a Completely Randomized Design (CRD) with 1 control variable, namely no mixing of microplastics in commercial feeds and 3 different doses of microplastics, namely A (5%), B (10%), C (15%) mixed with commercial feed, where each treatment was repeated 3 times. The results of the one way ANOVA analysis showed that the treatment of exposure to microplastics with different doses did not have a significant effect (P<0.05) on the survival rate and length growth of catfish. The survival rate ranges from 65- 100%. Survival values that were not significantly different could indicate that there was competition for space and dominance of larger fish in the aquarium which affected fish growth, feed utilization and fish survival. Meanwhile, the length growth value ranged from 21-28cm. The length growth value which was not significantly different could indicate that the dose of microplastic used in the study did not result in muscle and bone cell division which was the cause of the increase in body length of fish.
{"title":"Survival Rate and Growth Length of Catfish (Clarias gariepinus) Exposed to Microplastics","authors":"Diana Permatasari, Mohamad Fadjar, Ating Yuniarti","doi":"10.20473/jafh.v12i1.36844","DOIUrl":"https://doi.org/10.20473/jafh.v12i1.36844","url":null,"abstract":"Aquaculture has a land potential of 17.92 million ha consisting of 2.83 million ha of freshwater aquaculture potential, 2.96 million ha of brackish water aquaculture and 12.12 million ha of marine aquaculture. One of the freshwater species that has the potential to be cultivated is catfish (C. gariepinus). But now, as a result of the decline in the quality of the freshwater environment, catfish are prone to contamination by plastic waste. If microplastic particles have accumulated in large numbers in the fish's body, it can clog the fish's digestive tract and affect the growth of catfish which will affect the results of aquaculture production. The purpose of this study was to determine the survival rate or survival rate of catfish exposed to microplastics. The research design used in this study was a Completely Randomized Design (CRD) with 1 control variable, namely no mixing of microplastics in commercial feeds and 3 different doses of microplastics, namely A (5%), B (10%), C (15%) mixed with commercial feed, where each treatment was repeated 3 times. The results of the one way ANOVA analysis showed that the treatment of exposure to microplastics with different doses did not have a significant effect (P<0.05) on the survival rate and length growth of catfish. The survival rate ranges from 65- 100%. Survival values that were not significantly different could indicate that there was competition for space and dominance of larger fish in the aquarium which affected fish growth, feed utilization and fish survival. Meanwhile, the length growth value ranged from 21-28cm. The length growth value which was not significantly different could indicate that the dose of microplastic used in the study did not result in muscle and bone cell division which was the cause of the increase in body length of fish.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49072860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-18DOI: 10.20473/jafh.v12i1.37056
Desy Amalia Hidayati, A. Prajitno, Titik Dwi Sulistyawati, Giri Pratama, Tania Nilakandhi
This study aimed to analyze the antibacterial activity of red galangal extract (Alpinia purpurata) against the growth of E. tarda. This aims of this study was to determine the antibacterial compounds and the antibacterial effectiveness of red galangal extract (A. purpurata) against the growth of E. tarda bacteria. Antibacterial activity test was carried out by MIC test and disc test. The MIC test used doses of 1 ppm, 10 ppm, 100 ppm, 500 ppm, and 1000 ppm with incubation for 24 hours. The disc test used doses of 100 ppm, 200 ppm, 300 ppm, 400 ppm, 500 ppm and a positive control of 5 ppm chloramphenicol and a negative control without treatment. Photochemical test of red galangal extract (A. purpurata) contains flavonoid compounds, alkaloids, tannins, triterpenoids, and saponins. The results of the disc test showed that the highest inhibitory diameter was 7.55 mm at a dose of 500 ppm and was bacteriostatic because it decreased its inhibitory zone after 48 hours of incubation. The highest inhibitory effectiveness was 56.56% at a dose of 500 ppm after 24 hours of incubation.
{"title":"Antibacterial Activity of Red Galangal (A. purpurata) Extract on the Growth of E. tarda Bacteria","authors":"Desy Amalia Hidayati, A. Prajitno, Titik Dwi Sulistyawati, Giri Pratama, Tania Nilakandhi","doi":"10.20473/jafh.v12i1.37056","DOIUrl":"https://doi.org/10.20473/jafh.v12i1.37056","url":null,"abstract":"This study aimed to analyze the antibacterial activity of red galangal extract (Alpinia purpurata) against the growth of E. tarda. This aims of this study was to determine the antibacterial compounds and the antibacterial effectiveness of red galangal extract (A. purpurata) against the growth of E. tarda bacteria. Antibacterial activity test was carried out by MIC test and disc test. The MIC test used doses of 1 ppm, 10 ppm, 100 ppm, 500 ppm, and 1000 ppm with incubation for 24 hours. The disc test used doses of 100 ppm, 200 ppm, 300 ppm, 400 ppm, 500 ppm and a positive control of 5 ppm chloramphenicol and a negative control without treatment. Photochemical test of red galangal extract (A. purpurata) contains flavonoid compounds, alkaloids, tannins, triterpenoids, and saponins. The results of the disc test showed that the highest inhibitory diameter was 7.55 mm at a dose of 500 ppm and was bacteriostatic because it decreased its inhibitory zone after 48 hours of incubation. The highest inhibitory effectiveness was 56.56% at a dose of 500 ppm after 24 hours of incubation.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43593689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-18DOI: 10.20473/jafh.v12i1.29898
B. Takwin, Dimas Putra Perdana, Muhaemi Muktiniati, F. Azhar
The problem arises in vanamei shrimp cultivation is the emergence of diseases, such as vibriosis caused by Vibrio parahaemolyticus. Jatropha leaf extract can be used as an immunostimulant because it contains bioactive compounds namely flavonoid, tannins, saponins, and alkaloids. This research used a completely randomized design (CRD) with 5 treatments with 3 replications. The treatments used were; P1 (feed without extract and bacterial infection); P2 (feed without extract and NaCl injection); P3 : Feed + 1% + bacterial infection; P4 : Feed + 2% extract from feed and bacterial infection, P5 : Feed + 3% extract of feed and bacterial infection. Based on the results of the study, the administration of Jatropha leaf extract was able to increase the highest SR value of P2 by 88.3%, the highest THC value of P5 at 24.46 x 106 cells/ml, DHC values such as hyaline cells ranged from 36.67-71%, granular cells ranged from 17-38.57%, and semigranular cells ranged from 12-24.67%, the highest AF value in P5 was 77.25%, and the lowest TBC value was at P2 of 6 x 108 CFU/ml, the lowest TVC value was at P2 of 0.56 x 106 CFU/ml. The higher the dose of extract given, the higher the THC and AF, besides that, Jatropha leaves were also able to increase the SR value and suppress the growth of total bacteria and total Vibrio in shrimp intestines.
{"title":"Utilization of Jatropha Leaf (Jatropa curcas) Extract as Immune System Enhancer of Vannamei Shrimp (Litopenaeus vannamei) Infected with Vibrio parahaemolyticus","authors":"B. Takwin, Dimas Putra Perdana, Muhaemi Muktiniati, F. Azhar","doi":"10.20473/jafh.v12i1.29898","DOIUrl":"https://doi.org/10.20473/jafh.v12i1.29898","url":null,"abstract":"The problem arises in vanamei shrimp cultivation is the emergence of diseases, such as vibriosis caused by Vibrio parahaemolyticus. Jatropha leaf extract can be used as an immunostimulant because it contains bioactive compounds namely flavonoid, tannins, saponins, and alkaloids. This research used a completely randomized design (CRD) with 5 treatments with 3 replications. The treatments used were; P1 (feed without extract and bacterial infection); P2 (feed without extract and NaCl injection); P3 : Feed + 1% + bacterial infection; P4 : Feed + 2% extract from feed and bacterial infection, P5 : Feed + 3% extract of feed and bacterial infection. Based on the results of the study, the administration of Jatropha leaf extract was able to increase the highest SR value of P2 by 88.3%, the highest THC value of P5 at 24.46 x 106 cells/ml, DHC values such as hyaline cells ranged from 36.67-71%, granular cells ranged from 17-38.57%, and semigranular cells ranged from 12-24.67%, the highest AF value in P5 was 77.25%, and the lowest TBC value was at P2 of 6 x 108 CFU/ml, the lowest TVC value was at P2 of 0.56 x 106 CFU/ml. The higher the dose of extract given, the higher the THC and AF, besides that, Jatropha leaves were also able to increase the SR value and suppress the growth of total bacteria and total Vibrio in shrimp intestines.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43502319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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