Pub Date : 2023-06-16DOI: 10.20473/jafh.v12i3.31497
Nur Fauziyah Martiningsih, Uun Yanuhar, Mohammad Musa, Dwi Retna Kumalaningrum
This study examines the function of Brachionus sp. protein on the Cluster Differentiation-4 (CD4) immune system in cantang grouper infected with VNN. The purpose of this study was to determine the protein content of Brachionus sp. which has the potential as an antivirus and to find out the benefits of Brachionus sp. on the expression of CD4 as an indicator of increased fish body defense system against VNN. Brachionus sp. itself contains three protein bands with molecular weights of 122.73 kDa, 75.49 kDa, and 13.77 kDa, which are expected to increase the immune system against VNN on cantang grouper. Protein injection of Brachionus with doses of 35 µl, 105 µl and 170 µl /150 g cantang grouper, showed that the lowest decrease was at a dose of 105 µl/150 gram cantang groupers.
{"title":"Exploration of Brachionus sp. Protein on the In Vivo Response of CD4 at Cantang Grouper (Epinephelus sp.) with VNN Infection (Viral Nervous Necrosis)","authors":"Nur Fauziyah Martiningsih, Uun Yanuhar, Mohammad Musa, Dwi Retna Kumalaningrum","doi":"10.20473/jafh.v12i3.31497","DOIUrl":"https://doi.org/10.20473/jafh.v12i3.31497","url":null,"abstract":"This study examines the function of Brachionus sp. protein on the Cluster Differentiation-4 (CD4) immune system in cantang grouper infected with VNN. The purpose of this study was to determine the protein content of Brachionus sp. which has the potential as an antivirus and to find out the benefits of Brachionus sp. on the expression of CD4 as an indicator of increased fish body defense system against VNN. Brachionus sp. itself contains three protein bands with molecular weights of 122.73 kDa, 75.49 kDa, and 13.77 kDa, which are expected to increase the immune system against VNN on cantang grouper. Protein injection of Brachionus with doses of 35 µl, 105 µl and 170 µl /150 g cantang grouper, showed that the lowest decrease was at a dose of 105 µl/150 gram cantang groupers.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":"73 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135671119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-16DOI: 10.20473/jafh.v12i3.17949
Dr. Woro Hastuti Satyantini, Ir., M.Si, Ahmad Syahrul Mubarak, Sri Subekti
One of the obstacles often encountered in white shrimp farming is the presence of bacterial diseases caused by Vibrio group bacteria. One of the efforts to control Vibriosis is by using antagonistic bacteria as biocontrol agents, one of which is using Pseudomonas diminuta. In this study Pseudomonas diminuta and Vibrio harveyi were co-cultured with the aim of determining the optimal density and effective incubation time of P. diminuta which could provide the highest inhibition of V. harveyi growth. This study used a completely randomized design with six treatments P0 (P. diminuta 105 CFU/ml), P1 (V. harveyi 106 CFU/ml), P2 (P. diminuta 105 CFU/ml+V. harveyi 106 CFU/ml), P3 (P. diminuta 106 CFU/ml+V. harveyi 106 CFU/ml), P4 (P. diminuta 107 CFU/ml+V. harveyi 106 CFU/ml), P5 (P. diminuta 108 CFU/ml+V. harveyi 106 CFU/ml). The results of this study showed that P2, P3, P4 and P5 had decreased growth of V. harveyi when compared to control P1 (V.harveyi 106 CFU/ml). The decrease in the growth of V. harveyi occurred at the 8th to 48th hour. V. harveyi in P3 (2.72 × 108 CFU/ml), P2 (2.80 × 108 CFU/ml), P4 (2.96 × 108 CFU/ml) and P5 (2.90 × 108 CFU/ml) at the 48th hour of incubation was lower than P1 (3, 15×108 CFU/ml). Based on the results of the Duncan test showed that P2, P3, P4 and P5 were significantly different (p <0.05) from P1. From this study it was concluded that administration of P. diminuta at a density of 106 CFU/ml was able to reduce the growth of V. harveyi. The greatest decrease in the growth of V. harveyi occurred at the 48th hour.
{"title":"Antagonism Pseudomonas diminuta on The Growth of Vibrio harveyi with Mix Culture Method","authors":"Dr. Woro Hastuti Satyantini, Ir., M.Si, Ahmad Syahrul Mubarak, Sri Subekti","doi":"10.20473/jafh.v12i3.17949","DOIUrl":"https://doi.org/10.20473/jafh.v12i3.17949","url":null,"abstract":"One of the obstacles often encountered in white shrimp farming is the presence of bacterial diseases caused by Vibrio group bacteria. One of the efforts to control Vibriosis is by using antagonistic bacteria as biocontrol agents, one of which is using Pseudomonas diminuta. In this study Pseudomonas diminuta and Vibrio harveyi were co-cultured with the aim of determining the optimal density and effective incubation time of P. diminuta which could provide the highest inhibition of V. harveyi growth. This study used a completely randomized design with six treatments P0 (P. diminuta 105 CFU/ml), P1 (V. harveyi 106 CFU/ml), P2 (P. diminuta 105 CFU/ml+V. harveyi 106 CFU/ml), P3 (P. diminuta 106 CFU/ml+V. harveyi 106 CFU/ml), P4 (P. diminuta 107 CFU/ml+V. harveyi 106 CFU/ml), P5 (P. diminuta 108 CFU/ml+V. harveyi 106 CFU/ml). The results of this study showed that P2, P3, P4 and P5 had decreased growth of V. harveyi when compared to control P1 (V.harveyi 106 CFU/ml). The decrease in the growth of V. harveyi occurred at the 8th to 48th hour. V. harveyi in P3 (2.72 × 108 CFU/ml), P2 (2.80 × 108 CFU/ml), P4 (2.96 × 108 CFU/ml) and P5 (2.90 × 108 CFU/ml) at the 48th hour of incubation was lower than P1 (3, 15×108 CFU/ml). Based on the results of the Duncan test showed that P2, P3, P4 and P5 were significantly different (p <0.05) from P1. From this study it was concluded that administration of P. diminuta at a density of 106 CFU/ml was able to reduce the growth of V. harveyi. The greatest decrease in the growth of V. harveyi occurred at the 48th hour.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135671117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-27DOI: 10.20473/jafh.v12i2.35201
M. Kasnir, Khairun Nisaa, Agria Budi Darmawan, N. Farizah, I. Sudrajat
Tilapia fish have a salinity tolerance of 0 to 10 ppt. However, the increase in salinity causes a decrease in the growth rate of these fish. In addition, the rapid maturation of the gonads causes this fish to experience a slowdown in growth. This study aimed to know the effect of giving Melastoma malabathricum extract on the growth performance of tilapia fry reared in brackish water. The study consisted of treatment with 0, 1, 3, and 6 g/kg of feed with three replications of M. malabathricum leaf extract. M. malabathricum leaf extract was mixed in feed with a CMC concentration of 2%. Feed is given as much as 3% body weight twice a day. Observations of growth performance were carried out every seven days, and the amount of feed was readjusted based on the results of sampling fish weight. The study was conducted for 70 days using brackish water with a salinity of 8 ppt. Based on the study results, it was found that the highest growth of tilapia fry at the end of the study was found in the treatment of 1 g/kg feed. The same thing was also found in measuring feed efficiency, survival, and specific growth rate. It indicated that the best concentration of M. malabathricum leaf extract in supporting the growth performance of tilapia fry was 1 g/kg feed.
{"title":"Potential Bioactive Compounds of Melastoma malabathricium Leaf Extract in Feed on Growth Performance and Survival Rate of Tilapia Fry in Brackish Water","authors":"M. Kasnir, Khairun Nisaa, Agria Budi Darmawan, N. Farizah, I. Sudrajat","doi":"10.20473/jafh.v12i2.35201","DOIUrl":"https://doi.org/10.20473/jafh.v12i2.35201","url":null,"abstract":"Tilapia fish have a salinity tolerance of 0 to 10 ppt. However, the increase in salinity causes a decrease in the growth rate of these fish. In addition, the rapid maturation of the gonads causes this fish to experience a slowdown in growth. This study aimed to know the effect of giving Melastoma malabathricum extract on the growth performance of tilapia fry reared in brackish water. The study consisted of treatment with 0, 1, 3, and 6 g/kg of feed with three replications of M. malabathricum leaf extract. M. malabathricum leaf extract was mixed in feed with a CMC concentration of 2%. Feed is given as much as 3% body weight twice a day. Observations of growth performance were carried out every seven days, and the amount of feed was readjusted based on the results of sampling fish weight. The study was conducted for 70 days using brackish water with a salinity of 8 ppt. Based on the study results, it was found that the highest growth of tilapia fry at the end of the study was found in the treatment of 1 g/kg feed. The same thing was also found in measuring feed efficiency, survival, and specific growth rate. It indicated that the best concentration of M. malabathricum leaf extract in supporting the growth performance of tilapia fry was 1 g/kg feed.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48513309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-27DOI: 10.20473/jafh.v12i2.35022
R. Prasetyo, A. Mubarak, E. Saputra, J. Triastuti
Collagen is a connective tissue protein that is mostly produced from cattle and pigs. The use of cows and pigs as the manufacture of collagen causes a disease case, among others are bovine spongiform encephalopathy and transmissible spongiform disease. One of the alternative materials in the isolation of collagen is the use of fish skin. The purpose of this study was to determine the effect of the concentration of acetic acid solution on the characteristics of red snapper collagen produced by the hydroextraction method and to determine the optimum concentration of acetic acid solution in the red snapper skin collagen isolation process using the hydroextraction method. This study was experimental with Completely Randomized Design (CRD) consisting of 3 acetic acid treatment: (P1) acetic acid with concentration 0,1 M; (P2) 0,15 M and (P3) 0,2 M. This study showed that the use of different acetic acid had a significant effect (p<0.05) on the yield parameters that is 1.72 – 2.46%, water content 11.12 – 12.8%, and protein content 83.66 – 84.81% and had no significant effect on the pH. The best treatment was P3 which use the higher concentration of acetic acid that produced higher yield 2.46% and protein content of 84.81%. P3 with concentration of acetic acid 0,2 M can be used for found the best result of characterization of collagen.
{"title":"The Characterization of Collagen Isolated from Red Snapper Fish Skin (Lutjanus sp.) by Hydroextraction Method with Different Concentration of Acid Solution","authors":"R. Prasetyo, A. Mubarak, E. Saputra, J. Triastuti","doi":"10.20473/jafh.v12i2.35022","DOIUrl":"https://doi.org/10.20473/jafh.v12i2.35022","url":null,"abstract":"Collagen is a connective tissue protein that is mostly produced from cattle and pigs. The use of cows and pigs as the manufacture of collagen causes a disease case, among others are bovine spongiform encephalopathy and transmissible spongiform disease. One of the alternative materials in the isolation of collagen is the use of fish skin. The purpose of this study was to determine the effect of the concentration of acetic acid solution on the characteristics of red snapper collagen produced by the hydroextraction method and to determine the optimum concentration of acetic acid solution in the red snapper skin collagen isolation process using the hydroextraction method. This study was experimental with Completely Randomized Design (CRD) consisting of 3 acetic acid treatment: (P1) acetic acid with concentration 0,1 M; (P2) 0,15 M and (P3) 0,2 M. This study showed that the use of different acetic acid had a significant effect (p<0.05) on the yield parameters that is 1.72 – 2.46%, water content 11.12 – 12.8%, and protein content 83.66 – 84.81% and had no significant effect on the pH. The best treatment was P3 which use the higher concentration of acetic acid that produced higher yield 2.46% and protein content of 84.81%. P3 with concentration of acetic acid 0,2 M can be used for found the best result of characterization of collagen.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46681376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-27DOI: 10.20473/jafh.v12i2.34474
Ekaete Patrick Anwa-Udondiah, O. Okunade, A. S. Aboluwodi, I. O. Soboyejo, B. M. Lawal, I. Akintayo
This experiment investigated the impact of two water sources and their quality on the survival, growth and health of Clarias gariepinus fingerlings reared in indoor concrete tanks. 400 fingerlings of mean weight 15.33±3.47 g and total length 12.63±1.00 cm (mean±standard deviation) was randomly distributed into four concrete tanks of 12 m3 capacity each. Water quality was analyzed bi-weekly throughout study duration. Growth parameters such as mean weight gain (MWG), specific growth rate (SGR) and feed conversion rate (FCR) were calculated using standard procedures. At study termination, duplicate groups per treatment were assessed for survival rate and a health status check was determined by a hematological evaluation and microscopic examination of parasitic incidence. Results indicated that fish reared in water sourced from bore-hole [BH] performed comparatively significantly better in growth (p < 0.05) than fish in water from earthen pond [EP] as the MWG stood at 294.05±79.17 g for the former against 211.03±54.62 g for the latter with the survival rates for both treatments being in excess of 90%. Additionally, fish reared in EP presented with a higher infestation load of Trichodina spp., although there was no significant difference (U = 7, p > 0.05) between fish reared in EP and BH treatments with regards to their packed cell volume levels and white blood cells. The outcome of this study shed light on the impact a water source can have on the growth and health status of C. gariepinus with a recommendation of microbiological analysis of water supply from open/surface systems before usage for the African catfish aquaculture.
{"title":"Impact of Water Source and Quality on Survival, Growth and Health of Clarias gariepinus Fingerlings Reared in Indoor Concrete Tanks","authors":"Ekaete Patrick Anwa-Udondiah, O. Okunade, A. S. Aboluwodi, I. O. Soboyejo, B. M. Lawal, I. Akintayo","doi":"10.20473/jafh.v12i2.34474","DOIUrl":"https://doi.org/10.20473/jafh.v12i2.34474","url":null,"abstract":"This experiment investigated the impact of two water sources and their quality on the survival, growth and health of Clarias gariepinus fingerlings reared in indoor concrete tanks. 400 fingerlings of mean weight 15.33±3.47 g and total length 12.63±1.00 cm (mean±standard deviation) was randomly distributed into four concrete tanks of 12 m3 capacity each. Water quality was analyzed bi-weekly throughout study duration. Growth parameters such as mean weight gain (MWG), specific growth rate (SGR) and feed conversion rate (FCR) were calculated using standard procedures. At study termination, duplicate groups per treatment were assessed for survival rate and a health status check was determined by a hematological evaluation and microscopic examination of parasitic incidence. Results indicated that fish reared in water sourced from bore-hole [BH] performed comparatively significantly better in growth (p < 0.05) than fish in water from earthen pond [EP] as the MWG stood at 294.05±79.17 g for the former against 211.03±54.62 g for the latter with the survival rates for both treatments being in excess of 90%. Additionally, fish reared in EP presented with a higher infestation load of Trichodina spp., although there was no significant difference (U = 7, p > 0.05) between fish reared in EP and BH treatments with regards to their packed cell volume levels and white blood cells. The outcome of this study shed light on the impact a water source can have on the growth and health status of C. gariepinus with a recommendation of microbiological analysis of water supply from open/surface systems before usage for the African catfish aquaculture.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41550367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-27DOI: 10.20473/jafh.v12i2.38536
Nur Fidya Destianti, Himawan Prasetiyo, Ahmad Satibi, M. Rudi, Ferry Dwi Cahyadi, Agung Setyo Sasongko, Ardana Kurniaji
The parameter value of ornamental fish is determined by the brighter the color of a type of ornamental fish, the higher the value so that farmers need to maintain the color of the ornamental fish by providing feed containing color pigments that can be obtained from vegetable and animal sources of carotenoids. Sunkist balloon molly fish (Poecilia sp.) were stocked in an aquarium measuring 60 × 30 × 30 cm3 (5 fish/l), Objectives of this study was to determine the effect of feeding formulations using different carotenoid sources with the best treatment for improving the color quality of sunkist balloon molly fish (Poecilia sp.). The method used was experimental (quantitative) completely randomized design (CRD) with 3 treatments, 1 control, and 3 repetitions. Feed was given ad libitum (5% body weight) 3 times a day. Color quality data were analyzed using ImageJ Software and one-way ANOVA test. The results of the study showed that feed utilization efficiency, feed conversion ratio and growth performance were not significantly different between treatments. There was a significant effect on improving the quality of the color shown in the P₃ (yellow pumpkin) treatment of 10% as the best treatment among others. Water quality parameter that observed trend to be normal and stable from the beginning to the end of the maintenance period.
{"title":"Formulation of Feed with Different Source of Carotenoids on the Colors Quality of Sunkist Balloon Molly Fish (Poecilia sp.)","authors":"Nur Fidya Destianti, Himawan Prasetiyo, Ahmad Satibi, M. Rudi, Ferry Dwi Cahyadi, Agung Setyo Sasongko, Ardana Kurniaji","doi":"10.20473/jafh.v12i2.38536","DOIUrl":"https://doi.org/10.20473/jafh.v12i2.38536","url":null,"abstract":"The parameter value of ornamental fish is determined by the brighter the color of a type of ornamental fish, the higher the value so that farmers need to maintain the color of the ornamental fish by providing feed containing color pigments that can be obtained from vegetable and animal sources of carotenoids. Sunkist balloon molly fish (Poecilia sp.) were stocked in an aquarium measuring 60 × 30 × 30 cm3 (5 fish/l), Objectives of this study was to determine the effect of feeding formulations using different carotenoid sources with the best treatment for improving the color quality of sunkist balloon molly fish (Poecilia sp.). The method used was experimental (quantitative) completely randomized design (CRD) with 3 treatments, 1 control, and 3 repetitions. Feed was given ad libitum (5% body weight) 3 times a day. Color quality data were analyzed using ImageJ Software and one-way ANOVA test. The results of the study showed that feed utilization efficiency, feed conversion ratio and growth performance were not significantly different between treatments. There was a significant effect on improving the quality of the color shown in the P₃ (yellow pumpkin) treatment of 10% as the best treatment among others. Water quality parameter that observed trend to be normal and stable from the beginning to the end of the maintenance period.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42918005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-27DOI: 10.20473/jafh.v12i2.38647
Liza Nurohmah, Catur Sriherwanto, I. Suja’i, E. Yunita
Aquafeed biofloatation through tempeh mould fermentation on sinking aquafeeds produces water-floating property, an alternative to the expensive extrusion technique. However, the role of the fungal mycelium in this biofloatation remains unclear. This study aimed to investigate the role of surface mycelium of the fermented feed in the buoyancy. Commercial sinking feed was fermented using a tempeh starter at ambient temperature (28–33 °C) for 48 h. Freshly fermented feeds were produced, some of which were peeled to remove the surface mycelium, while the others were left intact. After 24-h oven-drying at 50 °C, physical tests were done on the peeled and unpeeled fermented feeds, plus unfermented feed as a negative control. Results showed that the unpeeled fermented feed had the highest floatability (48% at the 60th minute), and continued floating until the 120th minute with 36% floatability. In contrast, the unfermented feed did not float at all, while the peeled fermented feed sank within the first 2 minutes. Only the unpeeled fermented feed showed hydrophobic characteristics (> 90° contact angle and 20.16 s water absorption time). Thus, the hydrophobic surface mycelium might prevent rapid water infiltration into the fermented feed matrix, enabling the intact fermented feed to float longer.
{"title":"Aquafeed Biofloatation through Mycelial Hydrophobic Coating","authors":"Liza Nurohmah, Catur Sriherwanto, I. Suja’i, E. Yunita","doi":"10.20473/jafh.v12i2.38647","DOIUrl":"https://doi.org/10.20473/jafh.v12i2.38647","url":null,"abstract":"Aquafeed biofloatation through tempeh mould fermentation on sinking aquafeeds produces water-floating property, an alternative to the expensive extrusion technique. However, the role of the fungal mycelium in this biofloatation remains unclear. This study aimed to investigate the role of surface mycelium of the fermented feed in the buoyancy. Commercial sinking feed was fermented using a tempeh starter at ambient temperature (28–33 °C) for 48 h. Freshly fermented feeds were produced, some of which were peeled to remove the surface mycelium, while the others were left intact. After 24-h oven-drying at 50 °C, physical tests were done on the peeled and unpeeled fermented feeds, plus unfermented feed as a negative control. Results showed that the unpeeled fermented feed had the highest floatability (48% at the 60th minute), and continued floating until the 120th minute with 36% floatability. In contrast, the unfermented feed did not float at all, while the peeled fermented feed sank within the first 2 minutes. Only the unpeeled fermented feed showed hydrophobic characteristics (> 90° contact angle and 20.16 s water absorption time). Thus, the hydrophobic surface mycelium might prevent rapid water infiltration into the fermented feed matrix, enabling the intact fermented feed to float longer.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44391497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-27DOI: 10.20473/jafh.v12i2.33710
Jasmadi Jasmadi, C. D. Poeloengasih, H. Novianty, A. Sefrienda, S. Permadi, I. T. Suryaningtyas, B. Kumayanjati, D. Setyono
Ulva sp. is green macroalgae that very potential for producing food with high nutrients content. This species is found on a coastline in intertidal zone of Indonesia’s waters. There have been several research to this species start from post-harvest handling, nutrition, probiotic, and its ulvaran, however the information of effect of temperature storage to Ulva lactuca freshness is still lack. This study was undergone to evaluate the freshness quality of Ulva lactuca by sensory changes in different temperatures short-term storage. In order to evaluate the effect of the different storage circumstances, the fresh U. lactuca was collected from its natural habitat (intertidal zone of Sepanjang Coast, Yogyakarta Indonesia). It was rinsed from debris and epiphyte, stored in transparent polyethylene bag, and were stored in 4 °C, 15-20 °C and room temperature for five days. Color, pH, ash, moisture, crude protein, chlorophyl, Total Plate Count, sensory analysis was undergone along the storage period. The sensory evaluation score of U. lactuca in 4 °C were more than 6, and better than other storages. The crude protein (U. lactuca stored in 4 °C) decreased significantly on day 4 by 5.53%, it was lower than others. The TPC of all samples varied from 147x103in 0 days of storage to 2,462.5x103 CFU/ml on the last day of storage. In summaries, sensory scores of U. lactuca are more constant and higher in 4 °C than in other storages, despite minor nutrient content deterioration.
{"title":"Characteristic of Freshness Ulva lactuca Under Different Temperatures at Short-term Storage","authors":"Jasmadi Jasmadi, C. D. Poeloengasih, H. Novianty, A. Sefrienda, S. Permadi, I. T. Suryaningtyas, B. Kumayanjati, D. Setyono","doi":"10.20473/jafh.v12i2.33710","DOIUrl":"https://doi.org/10.20473/jafh.v12i2.33710","url":null,"abstract":"Ulva sp. is green macroalgae that very potential for producing food with high nutrients content. This species is found on a coastline in intertidal zone of Indonesia’s waters. There have been several research to this species start from post-harvest handling, nutrition, probiotic, and its ulvaran, however the information of effect of temperature storage to Ulva lactuca freshness is still lack. This study was undergone to evaluate the freshness quality of Ulva lactuca by sensory changes in different temperatures short-term storage. In order to evaluate the effect of the different storage circumstances, the fresh U. lactuca was collected from its natural habitat (intertidal zone of Sepanjang Coast, Yogyakarta Indonesia). It was rinsed from debris and epiphyte, stored in transparent polyethylene bag, and were stored in 4 °C, 15-20 °C and room temperature for five days. Color, pH, ash, moisture, crude protein, chlorophyl, Total Plate Count, sensory analysis was undergone along the storage period. The sensory evaluation score of U. lactuca in 4 °C were more than 6, and better than other storages. The crude protein (U. lactuca stored in 4 °C) decreased significantly on day 4 by 5.53%, it was lower than others. The TPC of all samples varied from 147x103in 0 days of storage to 2,462.5x103 CFU/ml on the last day of storage. In summaries, sensory scores of U. lactuca are more constant and higher in 4 °C than in other storages, despite minor nutrient content deterioration.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41566209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-27DOI: 10.20473/jafh.v12i2.39231
C. Huda, U. Yanuhar, M. Musa, Y. Wahyudi, N. S. Junirahma, N. Caesar
Cantang grouper (Epinephelus sp.) is one of the Export Oriented commodities which is a mainstay in increasing Indonesian mariculture production. The disease that causes many deaths in cantang grouper is Viral Nervous Necrosis. One of the most effective ways is by giving vaccines to fish. This study aims to develop a recombinant protein Chlorella vulgaris vaccine with the addition of an adjuvant to increase the immunity of cantang grouper infected with VNN. The method used was experimental with a Completely Randomized Design (CRD) consiting of seven treatments and three replications, namely (K+) cantang grouper infected with VNN, (T1) Healthy cantang grouper was given 33 µl of vaccine, (T2) Healthy cantang grouper was given vaccine of 66 µl, (T3) Healthy cantang grouper was given 112 µl of vaccine, (T4) Healthy cantang grouper was given 33 ul of vaccine and then tested against Viral Nervous Necrosis, (T5) Healthy cantang grouper was given 66 ul vaccine and then tested against Viral Nervous Necrosis, (T6) Healthy cantang grouper was given 112 ul vaccine and then tested against Viral Nervous Necrosis. The fish used were 8-10 cm in size and were reared for 56 days. The results showed that the Chlorella vulgaris protein recombinant vaccine with the addition of adjuvant gave a very significant effect on the immunity of cantang grouper infected with VNN with the best dose of 33 µl (T4) seen from the relative protection level (RPS) (75,6%), survival of cantang grouper (78%) after challenge test and a significant increase in antibody titer.
{"title":"Antibody Response in The Blood of Cantang Grouper Infected by Viral Nervous Necrosis with Recombinant Protein Chlorella Vulgaris Vaccination","authors":"C. Huda, U. Yanuhar, M. Musa, Y. Wahyudi, N. S. Junirahma, N. Caesar","doi":"10.20473/jafh.v12i2.39231","DOIUrl":"https://doi.org/10.20473/jafh.v12i2.39231","url":null,"abstract":"Cantang grouper (Epinephelus sp.) is one of the Export Oriented commodities which is a mainstay in increasing Indonesian mariculture production. The disease that causes many deaths in cantang grouper is Viral Nervous Necrosis. One of the most effective ways is by giving vaccines to fish. This study aims to develop a recombinant protein Chlorella vulgaris vaccine with the addition of an adjuvant to increase the immunity of cantang grouper infected with VNN. The method used was experimental with a Completely Randomized Design (CRD) consiting of seven treatments and three replications, namely (K+) cantang grouper infected with VNN, (T1) Healthy cantang grouper was given 33 µl of vaccine, (T2) Healthy cantang grouper was given vaccine of 66 µl, (T3) Healthy cantang grouper was given 112 µl of vaccine, (T4) Healthy cantang grouper was given 33 ul of vaccine and then tested against Viral Nervous Necrosis, (T5) Healthy cantang grouper was given 66 ul vaccine and then tested against Viral Nervous Necrosis, (T6) Healthy cantang grouper was given 112 ul vaccine and then tested against Viral Nervous Necrosis. The fish used were 8-10 cm in size and were reared for 56 days. The results showed that the Chlorella vulgaris protein recombinant vaccine with the addition of adjuvant gave a very significant effect on the immunity of cantang grouper infected with VNN with the best dose of 33 µl (T4) seen from the relative protection level (RPS) (75,6%), survival of cantang grouper (78%) after challenge test and a significant increase in antibody titer.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43338442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-27DOI: 10.20473/jafh.v12i2.31675
U. Enyidi, I. Ayogu
Phytogenic feed additives are plausible alternatives to antibiotics and serve as growth promoters in aquafeed. This research aims at finding the effects of varying inclusions of natural antioxidants like hibiscus (Hibiscus sabdariffa L.) and turmeric (Curcuma longa) on the growth, hematology, and nutrient utilization of Clarias gariepinus after 60 days feeding. This research used six feeds labeled as F1- F6 that varied in the composition of Curcuma longa and Hibiscus sabdariffa. The weight (g) inclusions of C. longa and H. sabdariffa were as follows, F1 0:250, F2 50:200, F3 100:15, F4 150:100, F5 200:50 and F6 250:0. Juvenile African catfish stocked at 9 catfish per 15L aquaria per 3 replicate aquariums (27/treatment feed) were fed for 60d. The catfish fed with F1 (250g H. sabdariffa) had the best specific growth rate 5.76±0.04 % day-1, lowest feed conversion ratio 1.01±0.01, weight gain 31.65±0.13 g, and protein efficiency ratio 0.96±0.07. The growth of African catfish increased with the increasing inclusion of hibiscus supplements. Hematobiochemical parameters ALT, AST was better for catfish fed high hibiscus supplements. We noticed that hibiscus inclusion was inversely proportional to cholesterol and total triglycerides levels of fish. The deposit of adipose tissues in the catfish was higher, with increasing inclusion of hibiscus than turmeric supplement.
{"title":"Substitution of Hibiscus sabdariffa with Curcuma longa in the Diets of Clarias gariepinus and the Effects on the Growth, Nutrient, and Hematobiochemistry","authors":"U. Enyidi, I. Ayogu","doi":"10.20473/jafh.v12i2.31675","DOIUrl":"https://doi.org/10.20473/jafh.v12i2.31675","url":null,"abstract":"Phytogenic feed additives are plausible alternatives to antibiotics and serve as growth promoters in aquafeed. This research aims at finding the effects of varying inclusions of natural antioxidants like hibiscus (Hibiscus sabdariffa L.) and turmeric (Curcuma longa) on the growth, hematology, and nutrient utilization of Clarias gariepinus after 60 days feeding. This research used six feeds labeled as F1- F6 that varied in the composition of Curcuma longa and Hibiscus sabdariffa. The weight (g) inclusions of C. longa and H. sabdariffa were as follows, F1 0:250, F2 50:200, F3 100:15, F4 150:100, F5 200:50 and F6 250:0. Juvenile African catfish stocked at 9 catfish per 15L aquaria per 3 replicate aquariums (27/treatment feed) were fed for 60d. The catfish fed with F1 (250g H. sabdariffa) had the best specific growth rate 5.76±0.04 % day-1, lowest feed conversion ratio 1.01±0.01, weight gain 31.65±0.13 g, and protein efficiency ratio 0.96±0.07. The growth of African catfish increased with the increasing inclusion of hibiscus supplements. Hematobiochemical parameters ALT, AST was better for catfish fed high hibiscus supplements. We noticed that hibiscus inclusion was inversely proportional to cholesterol and total triglycerides levels of fish. The deposit of adipose tissues in the catfish was higher, with increasing inclusion of hibiscus than turmeric supplement.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44388455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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