Pub Date : 2024-02-28DOI: 10.20473/jafh.v13i1.43652
Eri Bachtiar, Ajeng Dinda Lestari, S. Astuty, Sunarto Sunarto, Fiddy Semba Prasetiya
Marennine is a blue-green pigment produced by diatoms of the Haslea genus, one of which is Haslea ostrearia. This marennine pigment is water-soluble and confirmed to contain polyphenols and glycosides. There are two forms of marennine pigment: the intracellular form of marennine (IMn) and the extracellular form of marennine (EMn). Marennine pigments exhibit various biological activities such as antibacterial, antiviral, antioxidant, allelopathic, and inhibiting the growth of other diatoms. With this biological activity, marennine can be used in various fields, one of which is aquaculture.This research aims to determine the level of toxicity and analyze the effect of exposure to the extracellular marennine produced by H. ostrearia on the juvenile blood clam Tegillarca granosa. By using the toxicity test method, Blue water which is supernatant of H. ostrearia (BW) was tested on juvenile of commercially important bivalve species, the blood clams Tegillarca granosa, with three treatments, namely control (0 mg/L), treatment A (0.25 mg/L) and treatment B (0.5 mg/L of BW). The observation parameters of this study were LC50-72 h with one-way ANOVA analysis. The results showed that the BW produced by H. ostrearia increased the survival of juvenile blood clams T. granosa up to 27.7% in treatment B (0.5 mg/L) compared to control within 72 h. The one-way ANOVA analysis revealed that the control and treatment B (0.5 mg/L) were significantly different. This study shows that there is potential to develop the use of marennine in shellfish aquaculture.
马钱子碱是由 Haslea 属硅藻（其中一种是 Haslea ostrearia）产生的一种蓝绿色色素。这种马钱子碱色素是水溶性的，经证实含有多酚和苷。马钱子碱色素有两种形式：细胞内形式的马钱子碱（IMn）和细胞外形式的马钱子碱（EMn）。马钱子碱色素具有多种生物活性，如抗菌、抗病毒、抗氧化、等位病理学作用以及抑制其他硅藻的生长。本研究旨在确定 H. ostrearia 产生的细胞外马钱子碱的毒性水平，并分析暴露于其产生的细胞外马钱子碱对血蛤幼体（Tegillarca granosa）的影响。本研究采用毒性试验法，以 H. ostrearia 的上清液（BW）为蓝水，对具有重要商业价值的双壳贝类--血蛤幼体进行了试验，试验分为三个处理，即对照组（0 mg/L）、处理 A（0.25 mg/L）和处理 B（0.5 mg/L BW）。本研究的观察参数为 LC50-72 h，采用单因素方差分析。结果表明，与对照组相比，处理 B（0.5 mg/L）中 H. ostrearia 产生的 BW 在 72 h 内可提高血蛤 T. granosa 幼体的存活率达 27.7%。这项研究表明，在贝类养殖中使用马钱子碱具有开发潜力。
{"title":"Effect of Extracellular Marennine produced by Haslea Ostrearia on the Blood Clams Tegillarca Granosa","authors":"Eri Bachtiar, Ajeng Dinda Lestari, S. Astuty, Sunarto Sunarto, Fiddy Semba Prasetiya","doi":"10.20473/jafh.v13i1.43652","DOIUrl":"https://doi.org/10.20473/jafh.v13i1.43652","url":null,"abstract":"Marennine is a blue-green pigment produced by diatoms of the Haslea genus, one of which is Haslea ostrearia. This marennine pigment is water-soluble and confirmed to contain polyphenols and glycosides. There are two forms of marennine pigment: the intracellular form of marennine (IMn) and the extracellular form of marennine (EMn). Marennine pigments exhibit various biological activities such as antibacterial, antiviral, antioxidant, allelopathic, and inhibiting the growth of other diatoms. With this biological activity, marennine can be used in various fields, one of which is aquaculture.This research aims to determine the level of toxicity and analyze the effect of exposure to the extracellular marennine produced by H. ostrearia on the juvenile blood clam Tegillarca granosa. By using the toxicity test method, Blue water which is supernatant of H. ostrearia (BW) was tested on juvenile of commercially important bivalve species, the blood clams Tegillarca granosa, with three treatments, namely control (0 mg/L), treatment A (0.25 mg/L) and treatment B (0.5 mg/L of BW). The observation parameters of this study were LC50-72 h with one-way ANOVA analysis. The results showed that the BW produced by H. ostrearia increased the survival of juvenile blood clams T. granosa up to 27.7% in treatment B (0.5 mg/L) compared to control within 72 h. The one-way ANOVA analysis revealed that the control and treatment B (0.5 mg/L) were significantly different. This study shows that there is potential to develop the use of marennine in shellfish aquaculture. ","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":"45 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140421606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-28DOI: 10.20473/jafh.v13i1.30596
Abdul Rahem Faqih, Febriyani Eka Supriatin, Aulia Rahmawati, S. Anitasari, Gilang Drajat Maulana, Muhammad Bachrun Alim
High demand on as consume fish nor as decorative fish, causes an increasing production. But, an obstacle occurs on production, due to reproductive characteristic of Common Carp fish. Different time on sperm and eggs production from male and female fish cause difficulties in breeding. Hence, it needed sperm preservation so the breeding could be done in whole year. Natural extenders are the solution to preserve fish sperm without side effect. The purpose of this study was to determine the effect of different types of extenders and different doses on the percentage of sperm motility and viability of carp (Cyprinus carpio) after storage This research was conducted at the Faculty of Fisheries and Marine Sciences, Universitas Brawijaya and the Freshwater Cultivation Installation (IBAT Punten), Batu, East Java. The method in this study was Complete Random Design (CRD) with 6 treatments, K (100ml lactate ringer); A (1ml cider + 99ml lactate ringer); B (1ml coconut + 99ml lactate ringer); C (1ml date juice + 99ml lactate ringer); D (1ml honey + 99ml lactate ringer); E (1ml sugar cane + 99ml lactate ringer). The results showed that the highest motility was obtained in the treatment of using date extract extender with a motility percentage rate of 77.66%; highest viability was obtained in the honey extract with 80.71%; and the highest fertility rate was obtained in the honey extract treatment with 72.67 %.
{"title":"Effect of Different Natural Extenders in Maintaining the Quality of Sperm Fish (Cyprinus carpio)","authors":"Abdul Rahem Faqih, Febriyani Eka Supriatin, Aulia Rahmawati, S. Anitasari, Gilang Drajat Maulana, Muhammad Bachrun Alim","doi":"10.20473/jafh.v13i1.30596","DOIUrl":"https://doi.org/10.20473/jafh.v13i1.30596","url":null,"abstract":"High demand on as consume fish nor as decorative fish, causes an increasing production. But, an obstacle occurs on production, due to reproductive characteristic of Common Carp fish. Different time on sperm and eggs production from male and female fish cause difficulties in breeding. Hence, it needed sperm preservation so the breeding could be done in whole year. Natural extenders are the solution to preserve fish sperm without side effect. The purpose of this study was to determine the effect of different types of extenders and different doses on the percentage of sperm motility and viability of carp (Cyprinus carpio) after storage This research was conducted at the Faculty of Fisheries and Marine Sciences, Universitas Brawijaya and the Freshwater Cultivation Installation (IBAT Punten), Batu, East Java. The method in this study was Complete Random Design (CRD) with 6 treatments, K (100ml lactate ringer); A (1ml cider + 99ml lactate ringer); B (1ml coconut + 99ml lactate ringer); C (1ml date juice + 99ml lactate ringer); D (1ml honey + 99ml lactate ringer); E (1ml sugar cane + 99ml lactate ringer). The results showed that the highest motility was obtained in the treatment of using date extract extender with a motility percentage rate of 77.66%; highest viability was obtained in the honey extract with 80.71%; and the highest fertility rate was obtained in the honey extract treatment with 72.67 %.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140419074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-28DOI: 10.20473/jafh.v13i1.49943
M. A. Alamsjah, Adibi Rahiman bin Md Nor
The active ingredients contained in seaweed prepare for pharmaceutical, cosmetic and food supplement needs from seaweed Sargassum sp. and Ulva sp. in different seasonal conditions it is thought to have a contribution of specific active ingredients. However, the fatty acid profile and morphology of the seaweed species Sargassum sp. obtained in the dry season and rainy season had the same results as the profile of the seaweed Ulva sp. which was collected in the Bangsring Beach area, Wongsorejo District, Banyuwangi, Indonesia. This is thought to be due to the extreme differences in dry season and rainy season conditions that have not yet resulted in real or drastic changes in the composition and quantity of macro and micronutrients, water quality and active fatty acid compounds as well as the adaptability of seaweed. Fatty acids of Sargassum sp. dominated by the composition of linoleic acid (C18:2), palmitic acid (C16:0) and oleic acid (C18:1), while the fatty acids of Ulva sp. dominated by the composition of heptadecanoic acid (C17:0), pentadecanoic acid (C15:0) and linoleic acid (C18:2n6).
{"title":"Identification of Fatty Acid Content of Sargassum sp. Seaweed and Ulva sp. in Different Seasonal Conditions","authors":"M. A. Alamsjah, Adibi Rahiman bin Md Nor","doi":"10.20473/jafh.v13i1.49943","DOIUrl":"https://doi.org/10.20473/jafh.v13i1.49943","url":null,"abstract":"The active ingredients contained in seaweed prepare for pharmaceutical, cosmetic and food supplement needs from seaweed Sargassum sp. and Ulva sp. in different seasonal conditions it is thought to have a contribution of specific active ingredients. However, the fatty acid profile and morphology of the seaweed species Sargassum sp. obtained in the dry season and rainy season had the same results as the profile of the seaweed Ulva sp. which was collected in the Bangsring Beach area, Wongsorejo District, Banyuwangi, Indonesia. This is thought to be due to the extreme differences in dry season and rainy season conditions that have not yet resulted in real or drastic changes in the composition and quantity of macro and micronutrients, water quality and active fatty acid compounds as well as the adaptability of seaweed. Fatty acids of Sargassum sp. dominated by the composition of linoleic acid (C18:2), palmitic acid (C16:0) and oleic acid (C18:1), while the fatty acids of Ulva sp. dominated by the composition of heptadecanoic acid (C17:0), pentadecanoic acid (C15:0) and linoleic acid (C18:2n6).","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":"44 21","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140419165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-28DOI: 10.20473/jafh.v13i1.42045
Dzikri Wahyudi, Mardjoko Mardjoko, Fitria Nawir, Gagan Garnawansah, Waluyo Waluyo, Sri Hidayati, Muhammad Ar Rozzaaq Nugraha
The snubnose pompano (Trachinotus blochii), which is an introduced fish, is currently in increasing demand, both for the domestic market (Jakarta and other big cities) and international markets such as Singapore, Taiwan, Hong Kong, and China. The snubnose pompano can be cultivated in ponds because it is tolerant to changes in salinity (5-40 ppt). In this study, the use of lights installed in cages used to maintain the snubnose pompano nursery will be responded to natural food that is positively phototactic (zooplankton, shrimp, and small fish species) which will gather as feed at night. So, it is hoped that the use of these organisms as an alternative feed source can increase feed efficiency. The urgency of this research is to determine efforts to save on the use of artificial feed and to suppress parasite attacks. A t-test was carried out to compare the averages of two different samples, on the growth rate and health of snubnose pompano fish. Research using lights resulted in optimum growth of snubnose pompano with 100% survival rate, feed conversion ratio (FCR) value of 0.9 and produced snubnose pompano size 80–100 g/head within two months as well as healthy fish seeds. With indicators of parasitic attack that are lower than the treatment without light with a prevalence value of 25% (with lights); range of 25%-75% (without lights) and intensity 6-8 (with lights); range 11-35.67 (without light). The snubnose pompano seeds are always actively moving when the light is on, the use of the light can effectively catch organisms such as small shrimp or small crustaceans so that the snubnose pompano seeds can be used to be digested as an alternative food (as observed in the stomach contents and intestines of the snubnose pompano). The light also indicates that parasitic attacks on the snubnose pompano are less common.
{"title":"Application Of Lights in The Nursery of Snubnose Pompano Trachinotus blochii (Lacepède, 1801) In Ponds as An Effort to Increase Feed Cost Efficiency","authors":"Dzikri Wahyudi, Mardjoko Mardjoko, Fitria Nawir, Gagan Garnawansah, Waluyo Waluyo, Sri Hidayati, Muhammad Ar Rozzaaq Nugraha","doi":"10.20473/jafh.v13i1.42045","DOIUrl":"https://doi.org/10.20473/jafh.v13i1.42045","url":null,"abstract":"The snubnose pompano (Trachinotus blochii), which is an introduced fish, is currently in increasing demand, both for the domestic market (Jakarta and other big cities) and international markets such as Singapore, Taiwan, Hong Kong, and China. The snubnose pompano can be cultivated in ponds because it is tolerant to changes in salinity (5-40 ppt). In this study, the use of lights installed in cages used to maintain the snubnose pompano nursery will be responded to natural food that is positively phototactic (zooplankton, shrimp, and small fish species) which will gather as feed at night. So, it is hoped that the use of these organisms as an alternative feed source can increase feed efficiency. The urgency of this research is to determine efforts to save on the use of artificial feed and to suppress parasite attacks. A t-test was carried out to compare the averages of two different samples, on the growth rate and health of snubnose pompano fish. Research using lights resulted in optimum growth of snubnose pompano with 100% survival rate, feed conversion ratio (FCR) value of 0.9 and produced snubnose pompano size 80–100 g/head within two months as well as healthy fish seeds. With indicators of parasitic attack that are lower than the treatment without light with a prevalence value of 25% (with lights); range of 25%-75% (without lights) and intensity 6-8 (with lights); range 11-35.67 (without light). The snubnose pompano seeds are always actively moving when the light is on, the use of the light can effectively catch organisms such as small shrimp or small crustaceans so that the snubnose pompano seeds can be used to be digested as an alternative food (as observed in the stomach contents and intestines of the snubnose pompano). The light also indicates that parasitic attacks on the snubnose pompano are less common.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":"16 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140423055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-18DOI: 10.20473/jafh.v12i3.43348
Ade Kurniawan, Abdul Gani, Endang Muhammad, George M. Numberi, Endang Y. Papare, None Asti, Otto Sada
Feed was the most important part in aquaculture. Not only affecting growth, but the feed also impacted the cultivation environment. Poor feeding management in the use of feed will have a negative impact on the cultivation process. The purpose of the research was to create a dynamic system model. In the model a systemized algorithm was structured that would assist cultivators in carrying out aquaculture activities and with this model, problems that occurred were immediately resolved. The dynamic system model was the method used in this research. This model was used to determine the optimization of feed use based on several variables that support the process of using the feed. In this research, the production of Oreochromis niloticus was 14 kg, the amount of feed used was 10 kg, which affects the increase in the amount of organic matter, which was 3626.4 g (total feces + uneaten feed) in 65 days cultivation period. The result showed that the total feed was connected with organic waste, namely total N, total P, total feces, and total uneaten feed. The results obtained from this research were then poured into a dynamic system and the results were found, namely the relationship between feeding and organic waste, namely linear or perpendicular. The design of this dynamic system model was expected to enable tilapia cultivators to develop aquaculture businesses that were more environmentally friendly, effective, and efficient.
{"title":"Characteristics of Using Feed Impact on Tilapia (Oreochromis niloticus) Culture Using a Dynamics System Approach","authors":"Ade Kurniawan, Abdul Gani, Endang Muhammad, George M. Numberi, Endang Y. Papare, None Asti, Otto Sada","doi":"10.20473/jafh.v12i3.43348","DOIUrl":"https://doi.org/10.20473/jafh.v12i3.43348","url":null,"abstract":"Feed was the most important part in aquaculture. Not only affecting growth, but the feed also impacted the cultivation environment. Poor feeding management in the use of feed will have a negative impact on the cultivation process. The purpose of the research was to create a dynamic system model. In the model a systemized algorithm was structured that would assist cultivators in carrying out aquaculture activities and with this model, problems that occurred were immediately resolved. The dynamic system model was the method used in this research. This model was used to determine the optimization of feed use based on several variables that support the process of using the feed. In this research, the production of Oreochromis niloticus was 14 kg, the amount of feed used was 10 kg, which affects the increase in the amount of organic matter, which was 3626.4 g (total feces + uneaten feed) in 65 days cultivation period. The result showed that the total feed was connected with organic waste, namely total N, total P, total feces, and total uneaten feed. The results obtained from this research were then poured into a dynamic system and the results were found, namely the relationship between feeding and organic waste, namely linear or perpendicular. The design of this dynamic system model was expected to enable tilapia cultivators to develop aquaculture businesses that were more environmentally friendly, effective, and efficient.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135255804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
An important component in the application of biofloc is the presence of probiotic bacteria as a floc-forming agent. This study aimed to analyze the differences in the yield of vannamei shrimp in a biofloc system using commercial probiotics and independent probiotics on a laboratory scale. The study design was a completely randomized design with 3 treatments and 4 replications, including independent probiotics (IP), commercial probiotics (CP) and control (C). PM utilized an independent formula with starter bacteria Lactobacillus casei and PK contained bacteria Bacillus sp., Pseudomonas sp., Nitrosomonas sp., Aerobacter sp., Nitrobacter sp. Probiotics were fermented 24 hours before its application to the maintenance medium. A total of 15 shrimp/aquarium measuring 3.29 ± 0.48 g were reared for 27 days after the floc was first formed. The results showed that the growth and feed conversion ratio of vannamei shrimp in the biofloc system were higher than that of the control (P<0.05). Growth and feed conversion ratio between probiotic treatments did not show significantly different results (P>0.05). Survival in treatment and control groups was not significantly different (P>0.05). The observed floc volumes which increased during the rearing of vannamei shrimp were up to 6.50-7.50 mL/L. Several types of organisms found in the flock included nematodes, phytoplankton, copepods and protozoa. The observed water quality was the same in each treatment except for higher dissolved oxygen and ammonia in the control. This study recommends the use of independent probiotics in vannamei shrimp culture based on biofloc technology.
{"title":"The Effect of Different Probiotic Sources on Vannamei Shrimp (Litopenaeus vannamei) Cultivation with Biofloc System","authors":"Ardana Kurniaji, Diana Putri Renitasari, Siti Aisyah Saridu, Anton Anton, Yunarty Yunarty","doi":"10.20473/jafh.v12i3.37996","DOIUrl":"https://doi.org/10.20473/jafh.v12i3.37996","url":null,"abstract":"An important component in the application of biofloc is the presence of probiotic bacteria as a floc-forming agent. This study aimed to analyze the differences in the yield of vannamei shrimp in a biofloc system using commercial probiotics and independent probiotics on a laboratory scale. The study design was a completely randomized design with 3 treatments and 4 replications, including independent probiotics (IP), commercial probiotics (CP) and control (C). PM utilized an independent formula with starter bacteria Lactobacillus casei and PK contained bacteria Bacillus sp., Pseudomonas sp., Nitrosomonas sp., Aerobacter sp., Nitrobacter sp. Probiotics were fermented 24 hours before its application to the maintenance medium. A total of 15 shrimp/aquarium measuring 3.29 ± 0.48 g were reared for 27 days after the floc was first formed. The results showed that the growth and feed conversion ratio of vannamei shrimp in the biofloc system were higher than that of the control (P<0.05). Growth and feed conversion ratio between probiotic treatments did not show significantly different results (P>0.05). Survival in treatment and control groups was not significantly different (P>0.05). The observed floc volumes which increased during the rearing of vannamei shrimp were up to 6.50-7.50 mL/L. Several types of organisms found in the flock included nematodes, phytoplankton, copepods and protozoa. The observed water quality was the same in each treatment except for higher dissolved oxygen and ammonia in the control. This study recommends the use of independent probiotics in vannamei shrimp culture based on biofloc technology.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135255811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-18DOI: 10.20473/jafh.v12i3.40256
Gian Suryanatha Hartawan, Uun Yanuhar, Muhammad Musa, None Amira Baihani, None Yusuf Arif Wahyudi, None Choirul Huda, None Nico Rahman Caesar
Viral Nervous Necrosis (VNN) and Vibriosis are contagious diseases that can affect grouper fish from the larval to adult stages and cause significant economic losses for farmers. The owner brought the sunuk grouper to BKIPM Mataram to determine the fish's health. Since the grouper arrived at the floating net cage, the owner reports it has sustained bodily injuries. The grouper is separated from the healthy fish and placed in the area where the sick fish are. During maintenance, the fish exhibits a decrease in appetite, wounds on the mouth, operculum, and body from the head to the tail, low mobility, and swimming upside-down. The physical examination revealed that the fish was swimming limply in an inverted position and had lesions on various body parts. According to virological and bacteriological laboratory tests, the sunuk grouper infected with Vibriosis and VNN can be affected by the condition of fish that have been sick since they first arrived. Also, stress, contact with infected fish, and a decreased appetite result in sunuk grouper contracting the disease. Due to a disease, a fish's immune system does not function optimally. This condition makes sunuk susceptible to several infectious diseases.
{"title":"Viral Nervous Necrosis and Vibriosis in Grouper Fish: A Case Study","authors":"Gian Suryanatha Hartawan, Uun Yanuhar, Muhammad Musa, None Amira Baihani, None Yusuf Arif Wahyudi, None Choirul Huda, None Nico Rahman Caesar","doi":"10.20473/jafh.v12i3.40256","DOIUrl":"https://doi.org/10.20473/jafh.v12i3.40256","url":null,"abstract":"Viral Nervous Necrosis (VNN) and Vibriosis are contagious diseases that can affect grouper fish from the larval to adult stages and cause significant economic losses for farmers. The owner brought the sunuk grouper to BKIPM Mataram to determine the fish's health. Since the grouper arrived at the floating net cage, the owner reports it has sustained bodily injuries. The grouper is separated from the healthy fish and placed in the area where the sick fish are. During maintenance, the fish exhibits a decrease in appetite, wounds on the mouth, operculum, and body from the head to the tail, low mobility, and swimming upside-down. The physical examination revealed that the fish was swimming limply in an inverted position and had lesions on various body parts. According to virological and bacteriological laboratory tests, the sunuk grouper infected with Vibriosis and VNN can be affected by the condition of fish that have been sick since they first arrived. Also, stress, contact with infected fish, and a decreased appetite result in sunuk grouper contracting the disease. Due to a disease, a fish's immune system does not function optimally. This condition makes sunuk susceptible to several infectious diseases.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":"179 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135255802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-18DOI: 10.20473/jafh.v12i3.35007
Muhammad Septian Azhar Siregar, Eri Bachtiar, Atikah Nurhayati, Muhammad Wahyudi Lewaru
The frequent use of synthetic antioxidant compounds can cause degenerative diseases, especially consumers, so that natural antioxidant compounds are needed to reduce the risk of disease. One of the biotas that may contain potential as natural antioxidants is sea cucumber. Sea cucumbers are marine invertebrates that have therapeutic properties whose bioactive content has the potential as antioxidants. Thus, this study aims to determine the potential of two species of sea cucumber (Stichopus variegatus and Holothuria fuscocinerea) as natural antioxidant candidates as an alternative to synthetic antioxidants by knowing the bioactive content and analyzing the antioxidant activity of the sample. The research was started from April 1 to September 29, 2021 with methods including sampling in the waters of the Thousand Islands National Park, extraction, antioxidant testing, testing for total compound content, and data analysis on sample extracts of S. variegatus and H. fuscocinerea. The results of the DDPH antioxidant test and β-carotene bleaching assay after the one-way ANOVA test showed that the antioxidant activity value was P < 0.05, which means that there was a significant difference in sample concentration on antioxidant activity. In the method and -carotene bleaching assay, the sample extracts of S. variegatus and H. fuscocinerea were 46.37% and 45.75%, respectively. Based on the value of the antioxidant test results and bioactive content, it can be concluded that the sample extracts of S. variegatus and H. fuscocinerea have very weak antioxidant activity so that they cannot be used as alternative natural antioxidants to replace synthetic antioxidants.
{"title":"Antioxidant Activity of Gamat (Stichopus variegatus) and Milk Sea Cucumbers (Holothuria fuscocinerea) from the Thousand Islands National Park Waters","authors":"Muhammad Septian Azhar Siregar, Eri Bachtiar, Atikah Nurhayati, Muhammad Wahyudi Lewaru","doi":"10.20473/jafh.v12i3.35007","DOIUrl":"https://doi.org/10.20473/jafh.v12i3.35007","url":null,"abstract":"The frequent use of synthetic antioxidant compounds can cause degenerative diseases, especially consumers, so that natural antioxidant compounds are needed to reduce the risk of disease. One of the biotas that may contain potential as natural antioxidants is sea cucumber. Sea cucumbers are marine invertebrates that have therapeutic properties whose bioactive content has the potential as antioxidants. Thus, this study aims to determine the potential of two species of sea cucumber (Stichopus variegatus and Holothuria fuscocinerea) as natural antioxidant candidates as an alternative to synthetic antioxidants by knowing the bioactive content and analyzing the antioxidant activity of the sample. The research was started from April 1 to September 29, 2021 with methods including sampling in the waters of the Thousand Islands National Park, extraction, antioxidant testing, testing for total compound content, and data analysis on sample extracts of S. variegatus and H. fuscocinerea. The results of the DDPH antioxidant test and β-carotene bleaching assay after the one-way ANOVA test showed that the antioxidant activity value was P < 0.05, which means that there was a significant difference in sample concentration on antioxidant activity. In the method and -carotene bleaching assay, the sample extracts of S. variegatus and H. fuscocinerea were 46.37% and 45.75%, respectively. Based on the value of the antioxidant test results and bioactive content, it can be concluded that the sample extracts of S. variegatus and H. fuscocinerea have very weak antioxidant activity so that they cannot be used as alternative natural antioxidants to replace synthetic antioxidants.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":"39 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135255801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-18DOI: 10.20473/jafh.v12i3.31200
Jeremiah Olanipekun Jimoh, Bayode Paul Omobepade, Ademola Michael Akinsorotan, John Bunmi Olasunkanmi, Tolulope Omolayo Ariyomo, Victoria Oluwabunmi Fatoyinbo
This study investigated histopathological changes on the liver and gills of C. gariepinus juveniles treated intermittently with different concentrations of prophylactics. Potassium permanganate (T2), Hydrogen peroxide (T3) and Formalin (T4) were used as prophylactics and administered to 15 fish each at concentrations of 2 ppm, 150 ppm, and 100 ppm respectively. The treatments which were in three replicates each with a control set up (T1) were repeated biweekly for 10 weeks. The histomacrographs of the liver and the gills show that there were no visible alterations on the structures of the tissues of fish in the control experiment (T1). However, major histopathological observations for the fish in T2, T3, and T4 were severe degeneration in the gill structure, fusion of the gill rakers, slight changes in the hepatocytes, diffuse vacuolation of hepatocytes. Among the used chemicals, potassium permanganate appeared to have the least negative effects on the tissues and it is therefore recommended at 2 ppm for prophylactic treatment of fish against diseases.
{"title":"Histopathological Observations on Liver and Gills of Clarias gariepinus Juveniles Treated with Some Prophylactics","authors":"Jeremiah Olanipekun Jimoh, Bayode Paul Omobepade, Ademola Michael Akinsorotan, John Bunmi Olasunkanmi, Tolulope Omolayo Ariyomo, Victoria Oluwabunmi Fatoyinbo","doi":"10.20473/jafh.v12i3.31200","DOIUrl":"https://doi.org/10.20473/jafh.v12i3.31200","url":null,"abstract":"This study investigated histopathological changes on the liver and gills of C. gariepinus juveniles treated intermittently with different concentrations of prophylactics. Potassium permanganate (T2), Hydrogen peroxide (T3) and Formalin (T4) were used as prophylactics and administered to 15 fish each at concentrations of 2 ppm, 150 ppm, and 100 ppm respectively. The treatments which were in three replicates each with a control set up (T1) were repeated biweekly for 10 weeks. The histomacrographs of the liver and the gills show that there were no visible alterations on the structures of the tissues of fish in the control experiment (T1). However, major histopathological observations for the fish in T2, T3, and T4 were severe degeneration in the gill structure, fusion of the gill rakers, slight changes in the hepatocytes, diffuse vacuolation of hepatocytes. Among the used chemicals, potassium permanganate appeared to have the least negative effects on the tissues and it is therefore recommended at 2 ppm for prophylactic treatment of fish against diseases.","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135255809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-18DOI: 10.20473/jafh.v12i3.44446
Wiwin Sumiati, Gunanti Mahasri, Sri Subekti
Increased intensive cultivation of Punten Carp (Cyprinus carpio L.) is quite profitable, but on the other hand, it can result in a decrease in water quality due to uncontrolled leftover feed. Poor water quality can cause fish to become stressed then susceptible to disease, one of which is Myxobolus koi parasite infestation. The purpose of this study was to determine the effect of immunostimulant treatment of whole protein Myxobolus koi spores on the blood profile of Myxobolus-infested punten carp. The method used was an experiment with two treatments, the treatment without Myxobolus koi spore whole protein (P0) and the treatment of commercial feed added with an immunostimulant from Myxobolus koi spore whole protein of 5 µg/kg feed (P1). The study was conducted for 28 days and observed on days 0, 7, 14, 21, and 28, including the calculation of the number of erythrocytes, leukocytes, leukocyte differential, and water quality as supporting data. The abundance of erythrocytes in fish treated with P1 (1.16x106 cells/mm3-2.44x106 cells/mm3) was higher than in fish treated with P0 (1.09x106 cells/mm3-1.55x106 cells/mm3). The abundance of leukocytes in fish treated with P1 (3.79x104 cells/mm3-11.31x104 cells/mm3) was higher than in fish treated with P0 (3.51x104 cells/mm3-6.58x104 cells/mm3). The results of differential observations of leukocytes in the P0 treatment found basophils (0.3-1.3%), neutrophils (12.3-21.5%), eosinophils (2.5-4.9%), lymphocytes (61.9-76.8%) and monocytes (3.8-6.3%). The results of differential observations of leukocytes in the P1 treatment found basophils (0.3-1.0%), neutrophils (10.7-19.9%), eosinophils (2.4-3.9%), lymphocytes (61.8-72.6%) and monocytes (3.9-5.4%).
{"title":"Effect of Whole Protein Spore Myxobolus koi by Oral Treatment on Non-Specific Immune Response of Punten Carp (Cyprinus carpio L.) Infested with Myxobolus koi","authors":"Wiwin Sumiati, Gunanti Mahasri, Sri Subekti","doi":"10.20473/jafh.v12i3.44446","DOIUrl":"https://doi.org/10.20473/jafh.v12i3.44446","url":null,"abstract":"Increased intensive cultivation of Punten Carp (Cyprinus carpio L.) is quite profitable, but on the other hand, it can result in a decrease in water quality due to uncontrolled leftover feed. Poor water quality can cause fish to become stressed then susceptible to disease, one of which is Myxobolus koi parasite infestation. The purpose of this study was to determine the effect of immunostimulant treatment of whole protein Myxobolus koi spores on the blood profile of Myxobolus-infested punten carp. The method used was an experiment with two treatments, the treatment without Myxobolus koi spore whole protein (P0) and the treatment of commercial feed added with an immunostimulant from Myxobolus koi spore whole protein of 5 µg/kg feed (P1). The study was conducted for 28 days and observed on days 0, 7, 14, 21, and 28, including the calculation of the number of erythrocytes, leukocytes, leukocyte differential, and water quality as supporting data. The abundance of erythrocytes in fish treated with P1 (1.16x106 cells/mm3-2.44x106 cells/mm3) was higher than in fish treated with P0 (1.09x106 cells/mm3-1.55x106 cells/mm3). The abundance of leukocytes in fish treated with P1 (3.79x104 cells/mm3-11.31x104 cells/mm3) was higher than in fish treated with P0 (3.51x104 cells/mm3-6.58x104 cells/mm3). The results of differential observations of leukocytes in the P0 treatment found basophils (0.3-1.3%), neutrophils (12.3-21.5%), eosinophils (2.5-4.9%), lymphocytes (61.9-76.8%) and monocytes (3.8-6.3%). The results of differential observations of leukocytes in the P1 treatment found basophils (0.3-1.0%), neutrophils (10.7-19.9%), eosinophils (2.4-3.9%), lymphocytes (61.8-72.6%) and monocytes (3.9-5.4%).","PeriodicalId":15127,"journal":{"name":"Journal of Aquaculture and Fish Health","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135255800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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