Pub Date : 2022-08-25DOI: 10.21608/jbaar.2022.256197
N. Eskarous, Sobhy Hassab El-Nabi, M. A. Abd El Salam, K. Geba, S. G. GamalEl Din
: Background: DNA damage as Fragmentation has adverse effects on fertilization and embryo development, so it is one of the main causes of a male factor for infertility. Several techniques have been mentioned to elevation this damage. In our study, we determine DNA damage in human spermatozoa by sperm chromatin dispersion (SCD) method and Apoptosis of DNA in human spermatozoa by Optical density in gel electrophoresis in male infertility. Objects and Methods: Semen samples were collected from 100 men and were analyzed by standard light microscopic according to the World Organization (5 th edition) for diagnostic fertility. Furthermore, Sperm DNA damage was determined by using Halosperm Kit, then assessment apoptosis by optical density in Gel Electrophoresis. Results : The mean value of DNA by SCD method in infertile males increased with a value of 47.95±10.96 % when compared with the control value of 21.2 ±2.64 % with (p< 0.00001). On the other hand, the mean value of DNA by measurement of Optical density in Gel Electrophoresis in infertile males decreased with a value of 120.27±18.73 when compare with the control value of 144.4±45 with (p =0.833). Conclusion: by SCD method and other methods for detection of DNA apoptosis by gel electrophoresis addition to routine semen analysis play important role in the diagnosis and management of male infertility.
{"title":"Detection of DNA damage by SCD and Rate of Apoptosis DNA by Gel Electrophoresis among infertile males","authors":"N. Eskarous, Sobhy Hassab El-Nabi, M. A. Abd El Salam, K. Geba, S. G. GamalEl Din","doi":"10.21608/jbaar.2022.256197","DOIUrl":"https://doi.org/10.21608/jbaar.2022.256197","url":null,"abstract":": Background: DNA damage as Fragmentation has adverse effects on fertilization and embryo development, so it is one of the main causes of a male factor for infertility. Several techniques have been mentioned to elevation this damage. In our study, we determine DNA damage in human spermatozoa by sperm chromatin dispersion (SCD) method and Apoptosis of DNA in human spermatozoa by Optical density in gel electrophoresis in male infertility. Objects and Methods: Semen samples were collected from 100 men and were analyzed by standard light microscopic according to the World Organization (5 th edition) for diagnostic fertility. Furthermore, Sperm DNA damage was determined by using Halosperm Kit, then assessment apoptosis by optical density in Gel Electrophoresis. Results : The mean value of DNA by SCD method in infertile males increased with a value of 47.95±10.96 % when compared with the control value of 21.2 ±2.64 % with (p< 0.00001). On the other hand, the mean value of DNA by measurement of Optical density in Gel Electrophoresis in infertile males decreased with a value of 120.27±18.73 when compare with the control value of 144.4±45 with (p =0.833). Conclusion: by SCD method and other methods for detection of DNA apoptosis by gel electrophoresis addition to routine semen analysis play important role in the diagnosis and management of male infertility.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89909102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-22DOI: 10.21608/jbaar.2022.255697
F. Kadhum
Lactobacillus acidophilus represents a probiotic bacterium that may be found in the mouth, gut, and vaginal canal. Lactobacillus acidophillus may help to relieve diarrhea, bloating, and cramps caused by bacteria that may cause everything from diarrhea to life-threatening colon inflammation. Escherichia coli (hencefourth E. Coil ) bacteria are found in healthy people's intestines as natural flora. Only a very small percentage, like the E. coli O157:H7 variety, can lead to severe stomach discomfort, bloody diarrhea, and vomiting; the rest of the E. coli variety is harmless.
{"title":"Antimicrobial activity of Lactobacillus acidophilus against pathogenic Escherichia coli isolated from diarrhea patients","authors":"F. Kadhum","doi":"10.21608/jbaar.2022.255697","DOIUrl":"https://doi.org/10.21608/jbaar.2022.255697","url":null,"abstract":"Lactobacillus acidophilus represents a probiotic bacterium that may be found in the mouth, gut, and vaginal canal. Lactobacillus acidophillus may help to relieve diarrhea, bloating, and cramps caused by bacteria that may cause everything from diarrhea to life-threatening colon inflammation. Escherichia coli (hencefourth E. Coil ) bacteria are found in healthy people's intestines as natural flora. Only a very small percentage, like the E. coli O157:H7 variety, can lead to severe stomach discomfort, bloody diarrhea, and vomiting; the rest of the E. coli variety is harmless.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":"102 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80511374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-20DOI: 10.21608/jbaar.2022.255496
Mamoun Ghazalah, Sameer El-Masry, I. Helmy, Ehab Abd-Elkhalek
Background: Host genetic polymorphism is one of the major unalterable major factors for HCV infection, NF-κB proteins play multiple roles in immune response and involve in HCV infection and progression. Aim of the study: To investigate the associations between single nucleotide polymorphism (SNPs) in NF-Kb and the susceptibility as well as resolution of HCV infection. Patients and Methods: This prospective case-control study was conducted at the physical examination center on 150 Egyptian population, including 50 uninfected control cases, 50 cases with spontaneous viral clearance, and 50 cases with persistent HCV infection, they are genotyped for four SNPs (rs11820062, rs230530, rs1056890 and rs3774963) using a Taq Man assay. Results: The current study revealed that the mutation in rs_11820062 of the I kappa B alpha gene significantly increased the risk for HCV infection with a p-value <0.05. Conclusion: This study revealed that genetic variants of the NF-κB pathway genes (rs11820062 T allele) are associated with an increased risk of HCV susceptibility. light polypeptide gene enhancer
背景:宿主基因多态性是HCV感染的主要不可改变的主要因素之一，NF-κB蛋白在免疫应答中发挥多重作用，参与HCV感染和进展。研究目的:探讨NF-Kb单核苷酸多态性(snp)与HCV感染的易感性和消退之间的关系。患者和方法:本前瞻性病例对照研究在体检中心对150名埃及人群进行了研究，其中包括50例未感染的对照病例、50例自发性病毒清除病例和50例持续性HCV感染病例，采用Taq Man法对4个snp (rs11820062、rs230530、rs1056890和rs3774963)进行基因分型。结果:本研究发现I κ B α基因rs_11820062突变显著增加HCV感染的风险，p值<0.05。结论:本研究揭示NF-κB通路基因(rs11820062 T等位基因)的遗传变异与HCV易感性增加相关。轻型多肽基因增强子
{"title":"Genetic study of I kappa B alpha gene promoter polymorphism associated with hepatitis C virus in Egyptian patients","authors":"Mamoun Ghazalah, Sameer El-Masry, I. Helmy, Ehab Abd-Elkhalek","doi":"10.21608/jbaar.2022.255496","DOIUrl":"https://doi.org/10.21608/jbaar.2022.255496","url":null,"abstract":"Background: Host genetic polymorphism is one of the major unalterable major factors for HCV infection, NF-κB proteins play multiple roles in immune response and involve in HCV infection and progression. Aim of the study: To investigate the associations between single nucleotide polymorphism (SNPs) in NF-Kb and the susceptibility as well as resolution of HCV infection. Patients and Methods: This prospective case-control study was conducted at the physical examination center on 150 Egyptian population, including 50 uninfected control cases, 50 cases with spontaneous viral clearance, and 50 cases with persistent HCV infection, they are genotyped for four SNPs (rs11820062, rs230530, rs1056890 and rs3774963) using a Taq Man assay. Results: The current study revealed that the mutation in rs_11820062 of the I kappa B alpha gene significantly increased the risk for HCV infection with a p-value <0.05. Conclusion: This study revealed that genetic variants of the NF-κB pathway genes (rs11820062 T allele) are associated with an increased risk of HCV susceptibility. light polypeptide gene enhancer","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83196130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-08-20DOI: 10.21608/jbaar.2022.255500
Khalil Alhalfawy, Bahgat A. Elfiky, A. Zahran, Z. Kasemy, Mahmoud Zayed
{"title":"Stem cell therapy in renal diseases","authors":"Khalil Alhalfawy, Bahgat A. Elfiky, A. Zahran, Z. Kasemy, Mahmoud Zayed","doi":"10.21608/jbaar.2022.255500","DOIUrl":"https://doi.org/10.21608/jbaar.2022.255500","url":null,"abstract":"","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82102458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-28DOI: 10.21608/jbaar.2022.251726
Alyaa Majid, Ş. Adem, Anwer Kadhim
The acute coronary syndromes [unstable angina (UA) and acute myocardial infarction (AMI)] are more dangerous than other ischemic heart diseases (IHD) due to acute morphological changes in atherosclerotic plaques which cause (acute ischemia) severe imbalance between myocardium demand and oxygen supply. In this study we investigated adiponectin hormone, creatine kinase(CK), lactate dehydrogenase(LDH), malondialdehyde(MDA), nitric oxide(NO), superoxide dismutase(SOD), and glutathione(GSH) in patients with unstable angina and acute myocardial infarction, This study has been carried out on 100 patients with unstable angina and acute myocardial infarction and 50 healthy subjects. the study shows that there is a significant difference in the concentration of serum Adiponectin, CK, LDH, MDA, NO, SOD, and GSH between (controls, AMI and UA ) in the (male and female ) groups (p≤0.05).
{"title":"Evaluation of Adiponectin hormone and some biochemical parameters with unstable angina and acute myocardial infarction patients","authors":"Alyaa Majid, Ş. Adem, Anwer Kadhim","doi":"10.21608/jbaar.2022.251726","DOIUrl":"https://doi.org/10.21608/jbaar.2022.251726","url":null,"abstract":"The acute coronary syndromes [unstable angina (UA) and acute myocardial infarction (AMI)] are more dangerous than other ischemic heart diseases (IHD) due to acute morphological changes in atherosclerotic plaques which cause (acute ischemia) severe imbalance between myocardium demand and oxygen supply. In this study we investigated adiponectin hormone, creatine kinase(CK), lactate dehydrogenase(LDH), malondialdehyde(MDA), nitric oxide(NO), superoxide dismutase(SOD), and glutathione(GSH) in patients with unstable angina and acute myocardial infarction, This study has been carried out on 100 patients with unstable angina and acute myocardial infarction and 50 healthy subjects. the study shows that there is a significant difference in the concentration of serum Adiponectin, CK, LDH, MDA, NO, SOD, and GSH between (controls, AMI and UA ) in the (male and female ) groups (p≤0.05).","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77005893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-28DOI: 10.21608/jbaar.2022.251729
A. El-Adly, A. Wardany, Mohey Shikhoun
A pandemic-scale outbreak of the newly discovered coronavirus disease 2019 (COVID-19), fast-spreading viral pneumonia, is currently occurring. Due to the disease's overall vulnerability, different age groups have different clinical characteristics and test findings. The purpose of this study was to describe the COVID-19 laboratory results in various age and sex groups. Reverse transcriptase polymerase chain reaction (RT-PCR) for SARS-2 RNA was used in the study, which had 1100 individuals with typical cold symptoms. It was reported that 660 of these cases tested positive for the test, while 440 tested negatives, therefore all cases underwent laboratory testing. Our research revealed that males had higher COVID-19 positivity than females (215/660; 67.4%), with males scoring 445/660; 32.6%). Age does not statistically differ between COVID-19 positive and negative cases. Hematological parameters in blood cells revealed that Lymphocytes differ significantly between COVID-19-infected and uninfected patients as these cells decline in the presence of COVID-19 infection. There are no significant differences in hemoglobin (Hgb percent), red blood cells (RBCs), total white blood cells (WBCS), basophils, neutrophils, monocytes, and eosinophils, as well as blood platelets (PLTS). Erythrocyte sedimentation rate (ESR) is unimportant, whereas COVID-19 infection increases ferritin and C-reactive proteins.
{"title":"Biochemical changes in Egyptian patients infected with COVID-19","authors":"A. El-Adly, A. Wardany, Mohey Shikhoun","doi":"10.21608/jbaar.2022.251729","DOIUrl":"https://doi.org/10.21608/jbaar.2022.251729","url":null,"abstract":"A pandemic-scale outbreak of the newly discovered coronavirus disease 2019 (COVID-19), fast-spreading viral pneumonia, is currently occurring. Due to the disease's overall vulnerability, different age groups have different clinical characteristics and test findings. The purpose of this study was to describe the COVID-19 laboratory results in various age and sex groups. Reverse transcriptase polymerase chain reaction (RT-PCR) for SARS-2 RNA was used in the study, which had 1100 individuals with typical cold symptoms. It was reported that 660 of these cases tested positive for the test, while 440 tested negatives, therefore all cases underwent laboratory testing. Our research revealed that males had higher COVID-19 positivity than females (215/660; 67.4%), with males scoring 445/660; 32.6%). Age does not statistically differ between COVID-19 positive and negative cases. Hematological parameters in blood cells revealed that Lymphocytes differ significantly between COVID-19-infected and uninfected patients as these cells decline in the presence of COVID-19 infection. There are no significant differences in hemoglobin (Hgb percent), red blood cells (RBCs), total white blood cells (WBCS), basophils, neutrophils, monocytes, and eosinophils, as well as blood platelets (PLTS). Erythrocyte sedimentation rate (ESR) is unimportant, whereas COVID-19 infection increases ferritin and C-reactive proteins.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80109140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-07-04DOI: 10.21608/jbaar.2022.247840
Hayat Ahmed, N. Ali
: Background: Type 2 diabetes mellitus (DM2) is an important health problem worldwide affecting about 8 percent of the population. Diabetes Mellitus is one of the most common chronic diseases in Sudan. These patients have many changes in their blood, and many factors affect the glucose tolerance that is mediated by insulin, one of these changes is S.ferritin level, a marker of iron storage. Increased serum ferritin, reflecting body iron overload, is often associated with insulin resistance. The role of iron in the pathogenesis of diabetes is suggested by an increased incidence of type2 diabetes mellitus in diverse causes of iron overload, and reversal or improvement in glycemic control with a reduction in iron load achieved using either phlebotomy or iron-chelating therapy. Aim: To evaluate serum ferritin level in type-2 Diabetes Mellitus Sudanese Patients. Materials and Method: A case-control study was done in 45diabetic patients and 45normal healthy individuals as a control group, including measurement of serum ferritin by an electrochemiluminescence immunoassay (ECLIA) method full automated chemical analyzer. COBAS e411 machine used Roche HITACHI Kit and quantification of HbA1c level by i-chroma instrument. Results: The study showed that serum ferritin levels were significantly increased in diagnosed cases of type 2 diabetes mellitus in comparison with the age and gender-matched healthy individuals (P. value= 0.008). There was an insignificant correlation between serum ferritin and HbA1c(P. value=0.431). Conclusion: Therefore, the findings of the present study indicate that serum ferritin was increased in diabetes and this increase may contribute to the pathogenesis of this disease as well as to the development of complications. Thus, routine screening for serum ferritin concentration in pre-diabetes and diabetic patients should be done to assess the body's iron stores.
{"title":"Evaluation of Serum Ferritin in Type-2 Diabetes Mellitus Sudanese Patients","authors":"Hayat Ahmed, N. Ali","doi":"10.21608/jbaar.2022.247840","DOIUrl":"https://doi.org/10.21608/jbaar.2022.247840","url":null,"abstract":": Background: Type 2 diabetes mellitus (DM2) is an important health problem worldwide affecting about 8 percent of the population. Diabetes Mellitus is one of the most common chronic diseases in Sudan. These patients have many changes in their blood, and many factors affect the glucose tolerance that is mediated by insulin, one of these changes is S.ferritin level, a marker of iron storage. Increased serum ferritin, reflecting body iron overload, is often associated with insulin resistance. The role of iron in the pathogenesis of diabetes is suggested by an increased incidence of type2 diabetes mellitus in diverse causes of iron overload, and reversal or improvement in glycemic control with a reduction in iron load achieved using either phlebotomy or iron-chelating therapy. Aim: To evaluate serum ferritin level in type-2 Diabetes Mellitus Sudanese Patients. Materials and Method: A case-control study was done in 45diabetic patients and 45normal healthy individuals as a control group, including measurement of serum ferritin by an electrochemiluminescence immunoassay (ECLIA) method full automated chemical analyzer. COBAS e411 machine used Roche HITACHI Kit and quantification of HbA1c level by i-chroma instrument. Results: The study showed that serum ferritin levels were significantly increased in diagnosed cases of type 2 diabetes mellitus in comparison with the age and gender-matched healthy individuals (P. value= 0.008). There was an insignificant correlation between serum ferritin and HbA1c(P. value=0.431). Conclusion: Therefore, the findings of the present study indicate that serum ferritin was increased in diabetes and this increase may contribute to the pathogenesis of this disease as well as to the development of complications. Thus, routine screening for serum ferritin concentration in pre-diabetes and diabetic patients should be done to assess the body's iron stores.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83167980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-17DOI: 10.21608/jbaar.2022.244515
Farouk Hachem, H. Abdallah, A. Ibrahimi, H. Elsayegh, Y. Nouini
Lymphoepithelioma-like carcinoma is an undifferentiated carcinoma with histological features similar to undifferentiated, non-keratinizing carcinoma of the nasopharynx. Lymphoepithelioma-like carcinoma of the urinary bladder is uncommon with a reported incidence of 0.4%1.3% of all bladder cancer. We report a case of a 72 year-old-man with a muscle-invasive lymphoepithelioma-like carcinoma of the bladder who was treated with radical cystectomy with a 12-month follow-up.
{"title":"A Case Report of Lymphoepithelioma-like, a Variant of Urothelial Carcinoma of the Urinary Bladder","authors":"Farouk Hachem, H. Abdallah, A. Ibrahimi, H. Elsayegh, Y. Nouini","doi":"10.21608/jbaar.2022.244515","DOIUrl":"https://doi.org/10.21608/jbaar.2022.244515","url":null,"abstract":"Lymphoepithelioma-like carcinoma is an undifferentiated carcinoma with histological features similar to undifferentiated, non-keratinizing carcinoma of the nasopharynx. Lymphoepithelioma-like carcinoma of the urinary bladder is uncommon with a reported incidence of 0.4%1.3% of all bladder cancer. We report a case of a 72 year-old-man with a muscle-invasive lymphoepithelioma-like carcinoma of the bladder who was treated with radical cystectomy with a 12-month follow-up.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":"283 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73561868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-01DOI: 10.21608/jbaar.2022.246476
M. Fouda, B. Zarif, Victoria Samir, Sara A Mekkawy, Mohamed Omran
Previous research has linked an imbalance of the tissue inhibitors of metalloproteinase-1 (TIMP-1) and fibronectin (FN) to heart failure as a part of the extracellular matrix network (ECM) biochemistry profile, which is vital for cardiac homeostasis. This study aimed to assess the diagnostic performance of FN, TIMP-1, and CK-MB in heart failure (HF). Sixty patients (45 with acute and 15 with chronic HF) were recruited. Thirty individuals (20 with ischemic heart diseases, as other cardiac diseases, and 10 healthy individuals) were recruited as a control group. The biotin double antibody sandwich technology determined levels of human fibronectin and tissue inhibitors of metalloproteinase-1. FN was the most effective biomarker in differentiating HF patients from healthy individuals (AUC = 0.850) ( P < 0.001), followed by TIMP (AUC = 0.74) and CK MB (AUC = 0.660). The sensitivity and specificity of FN were 82% and 70%, respectively, at a cutoff of 80 ng/ml. In addition, FN and TIMP had the same AUC (0.71) and efficiency (65%) in distinguishing HF patients from controls, followed by CK-MB (AUC = 0.70). We developed a novel model for HF diagnosis named the HFD model based on three biomarkers (FN, TIMP, and CK MB). The HFD model had an AUC of 0.77 in distinguishing HF patients from healthy individuals, with a sensitivity, specificity, and accuracy reaching 80%. For differentiating HF patients from controls, the HFD model had 0.8 AUC, 76% sensitivity, 75% specificity, and 76% accuracy.
{"title":"Evaluation of the diagnostic performances of tissue inhibitors of metalloproteinase-1 and fibronectin for heart failure","authors":"M. Fouda, B. Zarif, Victoria Samir, Sara A Mekkawy, Mohamed Omran","doi":"10.21608/jbaar.2022.246476","DOIUrl":"https://doi.org/10.21608/jbaar.2022.246476","url":null,"abstract":"Previous research has linked an imbalance of the tissue inhibitors of metalloproteinase-1 (TIMP-1) and fibronectin (FN) to heart failure as a part of the extracellular matrix network (ECM) biochemistry profile, which is vital for cardiac homeostasis. This study aimed to assess the diagnostic performance of FN, TIMP-1, and CK-MB in heart failure (HF). Sixty patients (45 with acute and 15 with chronic HF) were recruited. Thirty individuals (20 with ischemic heart diseases, as other cardiac diseases, and 10 healthy individuals) were recruited as a control group. The biotin double antibody sandwich technology determined levels of human fibronectin and tissue inhibitors of metalloproteinase-1. FN was the most effective biomarker in differentiating HF patients from healthy individuals (AUC = 0.850) ( P < 0.001), followed by TIMP (AUC = 0.74) and CK MB (AUC = 0.660). The sensitivity and specificity of FN were 82% and 70%, respectively, at a cutoff of 80 ng/ml. In addition, FN and TIMP had the same AUC (0.71) and efficiency (65%) in distinguishing HF patients from controls, followed by CK-MB (AUC = 0.70). We developed a novel model for HF diagnosis named the HFD model based on three biomarkers (FN, TIMP, and CK MB). The HFD model had an AUC of 0.77 in distinguishing HF patients from healthy individuals, with a sensitivity, specificity, and accuracy reaching 80%. For differentiating HF patients from controls, the HFD model had 0.8 AUC, 76% sensitivity, 75% specificity, and 76% accuracy.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88819593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-06-01DOI: 10.21608/jbaar.2022.246929
A. Abbood
Preterm birth (PTB) and PE are the two major causes of perinatal mortality, morbidity, and long-range neurological disability. PTB defined as delivery before 37 weeks gestation, has become an epidemic in developed countries, Indeed PTB and PE are leading causes of maternal and neonatal death worldwide. The immunohistochemical study show: - The staining intensity for PE immunohistochemical showed the greatest (+2) intensity was recorded at 52% for anti-CD3 lymphocyte biomarker. Also, followed by 28% of (+1) Intensity was comparable biomarkers. (12%) of +3 and 8% for 0 intensity when compared with Tonsil control positive and placental tissue control negative. On the other hand, the staining intensity for PE immunohistochemical shows the greatest frequency of (0) intensity was recorded for anti-CD8 cytotoxic T-cell biomarker with 52 % then +1 (28%) and 20% for +2 when compared with skin control positive and negative.
{"title":"The Role of CD3 and CD8 in Preterm Preeclamptic women by using Immunohistochemical technique","authors":"A. Abbood","doi":"10.21608/jbaar.2022.246929","DOIUrl":"https://doi.org/10.21608/jbaar.2022.246929","url":null,"abstract":"Preterm birth (PTB) and PE are the two major causes of perinatal mortality, morbidity, and long-range neurological disability. PTB defined as delivery before 37 weeks gestation, has become an epidemic in developed countries, Indeed PTB and PE are leading causes of maternal and neonatal death worldwide. The immunohistochemical study show: - The staining intensity for PE immunohistochemical showed the greatest (+2) intensity was recorded at 52% for anti-CD3 lymphocyte biomarker. Also, followed by 28% of (+1) Intensity was comparable biomarkers. (12%) of +3 and 8% for 0 intensity when compared with Tonsil control positive and placental tissue control negative. On the other hand, the staining intensity for PE immunohistochemical shows the greatest frequency of (0) intensity was recorded for anti-CD8 cytotoxic T-cell biomarker with 52 % then +1 (28%) and 20% for +2 when compared with skin control positive and negative.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89815761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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