Pub Date : 2023-08-09DOI: 10.21608/jbaar.2023.311827
A. Yameny
: Coronavirus-19 is caused by “Severe acute respiratory syndrome coronavirus-2” (SARS-CoV-2), COVID-19 pandemic, has led to millions of confirmed cases and deaths worldwide. Efficient diagnostic tools are in high demand, as rapid and specific testing plays an important role in patient management and decelerating disease spread. the current technologies used to detect COVID-19 in clinical laboratories will be classified into main three categories, I - The polymerase chain reaction (PCR) method , II - Immunological or serological methods (Antigen and Antibody Detection ), III - Biochemical and hematological methods which include 1-Inflammatory biomarkers (Elevated and decreased group), 2. Multi-organ damage/failure (Cardiac, Hepatic, and Renal) .
{"title":"COVID-19 Laboratory diagnosis methods","authors":"A. Yameny","doi":"10.21608/jbaar.2023.311827","DOIUrl":"https://doi.org/10.21608/jbaar.2023.311827","url":null,"abstract":": Coronavirus-19 is caused by “Severe acute respiratory syndrome coronavirus-2” (SARS-CoV-2), COVID-19 pandemic, has led to millions of confirmed cases and deaths worldwide. Efficient diagnostic tools are in high demand, as rapid and specific testing plays an important role in patient management and decelerating disease spread. the current technologies used to detect COVID-19 in clinical laboratories will be classified into main three categories, I - The polymerase chain reaction (PCR) method , II - Immunological or serological methods (Antigen and Antibody Detection ), III - Biochemical and hematological methods which include 1-Inflammatory biomarkers (Elevated and decreased group), 2. Multi-organ damage/failure (Cardiac, Hepatic, and Renal) .","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76731492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-30DOI: 10.21608/jbaar.2023.305675
Naima Essouaadi, A. El aissami, M. Labhi̇li̇li̇, A. Al-Selwi, F. Bentata, Mohamed Benchacho
{"title":"Effect of medicinal plants on Rhynchosporium secalis","authors":"Naima Essouaadi, A. El aissami, M. Labhi̇li̇li̇, A. Al-Selwi, F. Bentata, Mohamed Benchacho","doi":"10.21608/jbaar.2023.305675","DOIUrl":"https://doi.org/10.21608/jbaar.2023.305675","url":null,"abstract":"","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77205767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-20DOI: 10.21608/jbaar.2023.299574
K. El-Said, Asmaa M. Mohamed, Amro E Mohamed
Conventional chemotherapy is an effective approach to cancer treatment. However, several side effects limited its uses. Natural products have been reported for their anticancer potential. This study evaluated the anticancer efficacy of Urtica pilulifera leaves extract (UPLE) alone or in combination with the Cis chemotherapeutic drug Cis in Ehrlich ascites carcinoma (EAC)-bearing mice. Phytochemical constituents were determined in UPLE by quantitative methods. Seventy mice were divided into seven groups (n = 10) as follows: Gp1 was used as a negative control, from Gp2 to Gp7 were inoculated with 1 × 10 6 EAC-cells/mouse, then Gp2 left as a positive control, Gp3 was injected with Cis (2 mg/kg), Gp4 was injected with UPLE (100 mg/kg), Gp5 was co-treated with Cis as Gp3 and UPLE as Gp4. Gp6 was injected with a low dose of Cis (0.5 mg/kg), and Gp7 was co-treated with a low dose of Cis as Gp6 and UPLE as Gp4. The body weight change percentages (b.wt%) were calculated. On day 14, all groups were sacrificed, the ascitic fluids were harvested, and the total tumor volume, count, and live and dead tumor cells were measured. Sera samples were collected for biochemical parameters assessment. Liver tissues were collected for the determination of oxidants/antioxidants biomarkers. The results showed that combinatorial treatment of the high or low doses of Cis with UPLE led to a synergistic effect on the reduction in the % b.wt changes due to the decrease in the ascitic tumor fluid. Cotreatment with Cis and UPLE exacerbates the antitumor efficacy of Cis with a significant decrease in liver dysfunctions induced by Cis and enhances the hepatic antioxidant status.
{"title":"Urtica pilulifera leaves exacerbate the cisplatin effect in Ehrlich ascites carcinoma-bearing mice","authors":"K. El-Said, Asmaa M. Mohamed, Amro E Mohamed","doi":"10.21608/jbaar.2023.299574","DOIUrl":"https://doi.org/10.21608/jbaar.2023.299574","url":null,"abstract":"Conventional chemotherapy is an effective approach to cancer treatment. However, several side effects limited its uses. Natural products have been reported for their anticancer potential. This study evaluated the anticancer efficacy of Urtica pilulifera leaves extract (UPLE) alone or in combination with the Cis chemotherapeutic drug Cis in Ehrlich ascites carcinoma (EAC)-bearing mice. Phytochemical constituents were determined in UPLE by quantitative methods. Seventy mice were divided into seven groups (n = 10) as follows: Gp1 was used as a negative control, from Gp2 to Gp7 were inoculated with 1 × 10 6 EAC-cells/mouse, then Gp2 left as a positive control, Gp3 was injected with Cis (2 mg/kg), Gp4 was injected with UPLE (100 mg/kg), Gp5 was co-treated with Cis as Gp3 and UPLE as Gp4. Gp6 was injected with a low dose of Cis (0.5 mg/kg), and Gp7 was co-treated with a low dose of Cis as Gp6 and UPLE as Gp4. The body weight change percentages (b.wt%) were calculated. On day 14, all groups were sacrificed, the ascitic fluids were harvested, and the total tumor volume, count, and live and dead tumor cells were measured. Sera samples were collected for biochemical parameters assessment. Liver tissues were collected for the determination of oxidants/antioxidants biomarkers. The results showed that combinatorial treatment of the high or low doses of Cis with UPLE led to a synergistic effect on the reduction in the % b.wt changes due to the decrease in the ascitic tumor fluid. Cotreatment with Cis and UPLE exacerbates the antitumor efficacy of Cis with a significant decrease in liver dysfunctions induced by Cis and enhances the hepatic antioxidant status.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80672237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-18DOI: 10.21608/jbaar.2023.298847
Aly Shoun, A. Mohamed, Gina Fouad, Abd-El Aziz El- Rifaie, N. Radi
{"title":"Assessment of cbiL gene expression and vitamin B12 levels in acne vulgaris: involvement of both in-vivo and in-vitro studies","authors":"Aly Shoun, A. Mohamed, Gina Fouad, Abd-El Aziz El- Rifaie, N. Radi","doi":"10.21608/jbaar.2023.298847","DOIUrl":"https://doi.org/10.21608/jbaar.2023.298847","url":null,"abstract":"","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90347849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-14DOI: 10.21608/jbaar.2023.285435
K. El-Said, Walaa Amoush, Amro E Mohamed
{"title":"Effect of Phoenix dactylifera seeds extract on cadmium-induced hepatotoxicity in male mice","authors":"K. El-Said, Walaa Amoush, Amro E Mohamed","doi":"10.21608/jbaar.2023.285435","DOIUrl":"https://doi.org/10.21608/jbaar.2023.285435","url":null,"abstract":"","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80055948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-13DOI: 10.21608/jbaar.2023.285279
Doaa Sultan, I. Mandour, K. Geba, A. Khallaf, N. Radwan
: Aim: Multiple sclerosis (MS) is an autoimmune disease with a controversial etiology. Both genetic and environmental factors are thought to be involved in the risk of developing the disease. The purpose of this study was to assess the association of the Vitamin D receptor (VDR) BsmI variant with MS and to investigate the interaction of this variant with vitamin D levels. Method: 100 subjects were recruited for this study. Fifty patients were diagnosed with MS and 50 were healthy individuals. BsmI was genotyped by polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) analyses in both groups and serum 25-hydroxyvitamin D [25(OH)D] levels were determined in MS patients by high-performance liquid chromatography (HPLC). Results: The distribution of the genotype of VDR polymorphism BsmI did not differ significantly between MS patients and healthy controls . The G allele of BsmI was a statistically significant higher percentage in MS patients ( p -value 0.045). There is no statistically significant difference in the level of 25(OH)D between MS patients and the control group. Conclusions: The study findings suggest that the VDR gene variant BsmI G allele may increase the risk of the development of MS.
{"title":"VITAMIN D AND VITAMIN D RECEPTOR GENE VARIANT IN EGYPTIAN MULTIPLE SCLEROSIS PATIENTS: A CASE-CONTROL STUDY","authors":"Doaa Sultan, I. Mandour, K. Geba, A. Khallaf, N. Radwan","doi":"10.21608/jbaar.2023.285279","DOIUrl":"https://doi.org/10.21608/jbaar.2023.285279","url":null,"abstract":": Aim: Multiple sclerosis (MS) is an autoimmune disease with a controversial etiology. Both genetic and environmental factors are thought to be involved in the risk of developing the disease. The purpose of this study was to assess the association of the Vitamin D receptor (VDR) BsmI variant with MS and to investigate the interaction of this variant with vitamin D levels. Method: 100 subjects were recruited for this study. Fifty patients were diagnosed with MS and 50 were healthy individuals. BsmI was genotyped by polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) analyses in both groups and serum 25-hydroxyvitamin D [25(OH)D] levels were determined in MS patients by high-performance liquid chromatography (HPLC). Results: The distribution of the genotype of VDR polymorphism BsmI did not differ significantly between MS patients and healthy controls . The G allele of BsmI was a statistically significant higher percentage in MS patients ( p -value 0.045). There is no statistically significant difference in the level of 25(OH)D between MS patients and the control group. Conclusions: The study findings suggest that the VDR gene variant BsmI G allele may increase the risk of the development of MS.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79158600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-11DOI: 10.21608/jbaar.2023.284928
S. El-Naggar, Mohamed A. Basyony, Seham El-Feki, Ramadan M. Kandyel
{"title":"Parotoid gland secretions of the Egyptian toad (Bufo relgularis): In vivo antitumor effect on Ehrlich ascites carcinoma bearing mice","authors":"S. El-Naggar, Mohamed A. Basyony, Seham El-Feki, Ramadan M. Kandyel","doi":"10.21608/jbaar.2023.284928","DOIUrl":"https://doi.org/10.21608/jbaar.2023.284928","url":null,"abstract":"","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89416429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-09DOI: 10.21608/jbaar.2023.284727
Abrar Mohamed, A. Hamza, H. Fadl, A. Albkrye, Hadeel Ahmed, Hazem A. Abubaker, S. Elbager
{"title":"In Silico approach for identification, prediction of AMPD1 gene nsSNPs associated with Myoadenylate Deaminase deficiency","authors":"Abrar Mohamed, A. Hamza, H. Fadl, A. Albkrye, Hadeel Ahmed, Hazem A. Abubaker, S. Elbager","doi":"10.21608/jbaar.2023.284727","DOIUrl":"https://doi.org/10.21608/jbaar.2023.284727","url":null,"abstract":"","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84805983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-04DOI: 10.21608/jbaar.2023.284031
S. Hasan, H. Aldik, Farah Haddad
{"title":"Study of the protective role of polyphenol antioxidants from extracted Damiana (Turnera diffusa Willd) against chlorpyrifos pesticide-induced toxicity in male rats","authors":"S. Hasan, H. Aldik, Farah Haddad","doi":"10.21608/jbaar.2023.284031","DOIUrl":"https://doi.org/10.21608/jbaar.2023.284031","url":null,"abstract":"","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88127855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-12DOI: 10.21608/jbaar.2022.273855
Roniefhel Apsay, Helen D. Alalag
In these chaotic, stressful, and uncertain times, nurse managers, who constantly receive new information about the disease and its prevention, work hard to make correct decisions while leading the nursing team. Managers must be ready to face the impact of the pandemic on nurses. The study answered the question; What are your experiences managing the hospital workforce during the COVID-19 pandemic? The study utilizes a phenomenological interpretative design approach in exploring the experiences of the nurse manager in the nursing workforce amid the pandemic. Participants were five currently working as a nurse manager who provided consent for an in-depth interview. The data was gathered through unstructured interviews. The researcher used a combination of snowball and convenient sampling to recruit the participants. Findings suggest that the study participants had developed resiliency in managing the nursing workforce. They find their experience during the pandemic with the following emerging themes: Looking back: the devastating events of the Covid 19 pertain to chaotic events of the pandemic, Managing the hospital resources and staffing, whereas they strategize to solve the shortcomings of the hospital because of understaffing and limited PPE for the staff nurses; Overcoming the challenges during COVID 19 strategic planning on massive hiring of nurses and proper training of level four PPE for Covid nurses; Psychological intervention among staff nurses during COVID 19 pandemic; Causes of the mental breakdown of the staff nurses and lastly mentoring the new generation nurse. A nurse manager requires a lot of strategizing in the allocation of resources, and the adaptability of every leader in the flow of a dire situation. The researcher recommends to the future researcher explore possible research designs of Grounded theory to develop a concept/theory of the journey of the nurse manager in managing the nursing workforce.
{"title":"The journey of nurse managers in the nursing workforce management during the Covid-19 pandemic","authors":"Roniefhel Apsay, Helen D. Alalag","doi":"10.21608/jbaar.2022.273855","DOIUrl":"https://doi.org/10.21608/jbaar.2022.273855","url":null,"abstract":"In these chaotic, stressful, and uncertain times, nurse managers, who constantly receive new information about the disease and its prevention, work hard to make correct decisions while leading the nursing team. Managers must be ready to face the impact of the pandemic on nurses. The study answered the question; What are your experiences managing the hospital workforce during the COVID-19 pandemic? The study utilizes a phenomenological interpretative design approach in exploring the experiences of the nurse manager in the nursing workforce amid the pandemic. Participants were five currently working as a nurse manager who provided consent for an in-depth interview. The data was gathered through unstructured interviews. The researcher used a combination of snowball and convenient sampling to recruit the participants. Findings suggest that the study participants had developed resiliency in managing the nursing workforce. They find their experience during the pandemic with the following emerging themes: Looking back: the devastating events of the Covid 19 pertain to chaotic events of the pandemic, Managing the hospital resources and staffing, whereas they strategize to solve the shortcomings of the hospital because of understaffing and limited PPE for the staff nurses; Overcoming the challenges during COVID 19 strategic planning on massive hiring of nurses and proper training of level four PPE for Covid nurses; Psychological intervention among staff nurses during COVID 19 pandemic; Causes of the mental breakdown of the staff nurses and lastly mentoring the new generation nurse. A nurse manager requires a lot of strategizing in the allocation of resources, and the adaptability of every leader in the flow of a dire situation. The researcher recommends to the future researcher explore possible research designs of Grounded theory to develop a concept/theory of the journey of the nurse manager in managing the nursing workforce.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74839653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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