Pub Date : 2019-10-01DOI: 10.21608/jbaar.2019.115401
M. Abdelhalim, M. Helal, D. El-Kady, N. Kamel, A. Sleem, O. Abdel-Salam, A. Abdallah, G. Elmegeed
The investigation of drugs used for the treatment of inflammatory diseases with limited side effects becomes an urgent need for inflammation patients. This search aimed at the preparation of novel hybrid heterosteroids with structures especially non-ulcerogenic and anti-inflammatory activities. The heterocyclic steroids were formulated using simple and effective techniques. IR, H NMR, C NMR spectra and elemental microanalysis were used to characterize the synthesized compound. The in vivo anti-inflammatory activity of some of these compounds was studied using carrageenan-induced paw oedema assay. Also, the effect of the different compounds on the development of gastric mucosal damage induced in rats by 96% EtOH administration was studied. The most marked and sustained inhibition of the oedema response was observed with the administration of the low and high doses (25 and 50 mg/kg) of compounds 8, 4, 23, 14 as well as by the high dose of compound 32. There was no significant difference in the degree of oedema inhibition between the low and high doses of compounds 8, 23, 14 at all-time points in the study. All investigated compounds administered at (50 mg/kg) dose inhibited gastric mucosal lesions induced in rats by 96% EtOH administration. Compounds 4, 9, 11, 26, 32 were the most effective in inhibiting lesion formation. No lesions were observed after the administration of compounds 9, 26 at 50 mg/kg. These findings are approaching a distinctive chance to create fresh anti-inflammatory drugs that eliminate the ulcerogenic liabilities connected with drugs presently on the market.
{"title":"Synthesis of Thiophenyl, Thiazolyl, and Pyridinyl Androstane Derivatives: A Novel Class of Potent Anti-inflammatory and Anti-ulcerogenic Heterocyclic Steroids","authors":"M. Abdelhalim, M. Helal, D. El-Kady, N. Kamel, A. Sleem, O. Abdel-Salam, A. Abdallah, G. Elmegeed","doi":"10.21608/jbaar.2019.115401","DOIUrl":"https://doi.org/10.21608/jbaar.2019.115401","url":null,"abstract":"The investigation of drugs used for the treatment of inflammatory diseases with limited side effects becomes an urgent need for inflammation patients. This search aimed at the preparation of novel hybrid heterosteroids with structures especially non-ulcerogenic and anti-inflammatory activities. The heterocyclic steroids were formulated using simple and effective techniques. IR, H NMR, C NMR spectra and elemental microanalysis were used to characterize the synthesized compound. The in vivo anti-inflammatory activity of some of these compounds was studied using carrageenan-induced paw oedema assay. Also, the effect of the different compounds on the development of gastric mucosal damage induced in rats by 96% EtOH administration was studied. The most marked and sustained inhibition of the oedema response was observed with the administration of the low and high doses (25 and 50 mg/kg) of compounds 8, 4, 23, 14 as well as by the high dose of compound 32. There was no significant difference in the degree of oedema inhibition between the low and high doses of compounds 8, 23, 14 at all-time points in the study. All investigated compounds administered at (50 mg/kg) dose inhibited gastric mucosal lesions induced in rats by 96% EtOH administration. Compounds 4, 9, 11, 26, 32 were the most effective in inhibiting lesion formation. No lesions were observed after the administration of compounds 9, 26 at 50 mg/kg. These findings are approaching a distinctive chance to create fresh anti-inflammatory drugs that eliminate the ulcerogenic liabilities connected with drugs presently on the market.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90450237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-09-28DOI: 10.21608/jbaar.2019.147698
H. Hameed
{"title":"Study the effect of pheromones on gene expression in the brain of the honeybee Apis mellifera","authors":"H. Hameed","doi":"10.21608/jbaar.2019.147698","DOIUrl":"https://doi.org/10.21608/jbaar.2019.147698","url":null,"abstract":"","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88916233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-16DOI: 10.21608/JBAAR.2019.147401
Mahmoud Sayed Ahmed Shahin
Background: Medication errors represent a serious problem in the hospital setting and remain a challenge to navigate among hospitalized patients in all departments. Mistakes in medication administration are considered a significant issue that threatens a patient’s safety and may increase their hospital stay, treatment costs, and mortality rate. Medication errors commonly committed by nurses may include medication preparation or administration errors, which are associated with the highest risk areas in nursing practice. Methodology: A pretest-posttest, quasi-experimental, the observational design was used. Convenience sampling was employed to include all intravenous medication errors committed by nurses in three ICUs of Jordan University Hospital (pretest: 236 errors and post-test: 68 errors, respectively). A designed incident report was used for data collection. Data collection was carried out simultaneously in the three ICUs during nurses’ preparation and administration of intravenous medications over two months for pretest and posttest data (May and June 2018). A tailored evidencedbased educational program designed using Phillips's Manual of I. V. Therapeutics: Evidence-based Practice for Infusion Therapy was furnished to all registered nurses utilizing structured classroom lectures and on-the-job training; moreover, educational medals of common medications and illustration posters were used as additional reminders.Results and Conclusion: More than half of nurses were females and held bachelor’s degrees. Half of the observed medication errors were identified in the surgical ICU. Intravenous medication errors observed during the day shift were significantly higher in number than those in the night shift. A significant reduction in the number of medication errors was noted after the implementation of a bundle of interventions (i.e., there was a reduction from 236 errors to 68 errors). Giving (1) an omeprazole push and then (2) administering vancomycin rapidly thereafter, followed by (3) administering omeprazole at the wrong time, were the three most observed medication BioBacta Journal of Bioscience and Applied Research
{"title":"Improving Intravenous Medication Administration and Reducing Medication Errors Among Critical Care Nurses at Jordan University Hospital","authors":"Mahmoud Sayed Ahmed Shahin","doi":"10.21608/JBAAR.2019.147401","DOIUrl":"https://doi.org/10.21608/JBAAR.2019.147401","url":null,"abstract":"Background: Medication errors represent a serious problem in the hospital setting and remain a challenge to navigate among hospitalized patients in all departments. Mistakes in medication administration are considered a significant issue that threatens a patient’s safety and may increase their hospital stay, treatment costs, and mortality rate. Medication errors commonly committed by nurses may include medication preparation or administration errors, which are associated with the highest risk areas in nursing practice. Methodology: A pretest-posttest, quasi-experimental, the observational design was used. Convenience sampling was employed to include all intravenous medication errors committed by nurses in three ICUs of Jordan University Hospital (pretest: 236 errors and post-test: 68 errors, respectively). A designed incident report was used for data collection. Data collection was carried out simultaneously in the three ICUs during nurses’ preparation and administration of intravenous medications over two months for pretest and posttest data (May and June 2018). A tailored evidencedbased educational program designed using Phillips's Manual of I. V. Therapeutics: Evidence-based Practice for Infusion Therapy was furnished to all registered nurses utilizing structured classroom lectures and on-the-job training; moreover, educational medals of common medications and illustration posters were used as additional reminders.Results and Conclusion: More than half of nurses were females and held bachelor’s degrees. Half of the observed medication errors were identified in the surgical ICU. Intravenous medication errors observed during the day shift were significantly higher in number than those in the night shift. A significant reduction in the number of medication errors was noted after the implementation of a bundle of interventions (i.e., there was a reduction from 236 errors to 68 errors). Giving (1) an omeprazole push and then (2) administering vancomycin rapidly thereafter, followed by (3) administering omeprazole at the wrong time, were the three most observed medication BioBacta Journal of Bioscience and Applied Research","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88755484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-07-29DOI: 10.21608/jbaar.2019.147394
Asma Ahmad Filimban, F. Al-Faraj, A. Oti
Waste produced from the end of useful life electrical and electronic equipment (EEE) denoted as e-waste, is one of the fastest waste streams worldwide. The growing amount of e-waste has caused a considerable challenge for policymakers to manage e-waste in an environmentally-sound mode in both developed and developing countries. Effective management of e-waste has become a global concern that is receiving growing attention due to the rapid increase in the quantity of e-waste. This is mainly attributed to the rapid technological innovation and shortening product’s useful lifespan coupled with the tendency of people to keep up with the advanced technologies. This study compared and critically appraised three e-waste management models (producer responsibility, not-producer responsibility, and sharing responsibility) currently applied in Malaysia and the United States of America (USA), in an attempt to explore best management practices for the collection and treatment of e-waste that can be adopted in the Kingdom of Saudi Arabia. The data presented in this paper are secondary data obtained from a wide range of authoritative sources. This study recommends developing an e-waste national policy and regulatory framework to effectively manage the rapidly growing rate of e-waste in the Kingdom of Saudi Arabia.
{"title":"Towards Sustainable Management of E-Waste in the Kingdom of Saudi Arabia: A Comparative Study of Three International Models","authors":"Asma Ahmad Filimban, F. Al-Faraj, A. Oti","doi":"10.21608/jbaar.2019.147394","DOIUrl":"https://doi.org/10.21608/jbaar.2019.147394","url":null,"abstract":"Waste produced from the end of useful life electrical and electronic equipment (EEE) denoted as e-waste, is one of the fastest waste streams worldwide. The growing amount of e-waste has caused a considerable challenge for policymakers to manage e-waste in an environmentally-sound mode in both developed and developing countries. Effective management of e-waste has become a global concern that is receiving growing attention due to the rapid increase in the quantity of e-waste. This is mainly attributed to the rapid technological innovation and shortening product’s useful lifespan coupled with the tendency of people to keep up with the advanced technologies. This study compared and critically appraised three e-waste management models (producer responsibility, not-producer responsibility, and sharing responsibility) currently applied in Malaysia and the United States of America (USA), in an attempt to explore best management practices for the collection and treatment of e-waste that can be adopted in the Kingdom of Saudi Arabia. The data presented in this paper are secondary data obtained from a wide range of authoritative sources. This study recommends developing an e-waste national policy and regulatory framework to effectively manage the rapidly growing rate of e-waste in the Kingdom of Saudi Arabia.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82992557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-05-11DOI: 10.21608/jbaar.2019.141365
A. Abdi, Mohd Salleh
Knowledge, Attitude and Practice toward Prevention of Hepatitis B Virus Infection among Somalian Immigrant in the State of Selangor, Malaysia, and their HBV Infection Status Abdirauf Mohamed Abdi, Mohd Nazil Salleh Department of Health Sciences, Faculty of Engineering and Life Sciences, Universiti Selangor, Shah Alam City Campus, Jalan Zikron A 7/A, Seksyen 7, 40000 Shah Alam Selangor, Malaysia *Corresponding author: Associate Professor Dr. Mohd Nazil Salleh, Department of Health Sciences, Faculty of Engineering and Life Sciences, Universiti Selangor, Shah Alam City Campus, Jalan Zikron A 7/A, Seksyen 7, 40000 Shah Alam Selangor, Malaysia. Email: drnazil@unisel.edu.my. Tel: +603 -5522 3471
{"title":"Knowledge, Attitude and Practice toward Prevention of Hepatitis B Virus Infection among Somalian Immigrant in the State of Selangor, Malaysia, and their HBV Infection Status","authors":"A. Abdi, Mohd Salleh","doi":"10.21608/jbaar.2019.141365","DOIUrl":"https://doi.org/10.21608/jbaar.2019.141365","url":null,"abstract":"Knowledge, Attitude and Practice toward Prevention of Hepatitis B Virus Infection among Somalian Immigrant in the State of Selangor, Malaysia, and their HBV Infection Status Abdirauf Mohamed Abdi, Mohd Nazil Salleh Department of Health Sciences, Faculty of Engineering and Life Sciences, Universiti Selangor, Shah Alam City Campus, Jalan Zikron A 7/A, Seksyen 7, 40000 Shah Alam Selangor, Malaysia *Corresponding author: Associate Professor Dr. Mohd Nazil Salleh, Department of Health Sciences, Faculty of Engineering and Life Sciences, Universiti Selangor, Shah Alam City Campus, Jalan Zikron A 7/A, Seksyen 7, 40000 Shah Alam Selangor, Malaysia. Email: drnazil@unisel.edu.my. Tel: +603 -5522 3471","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80887734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-05-06DOI: 10.21608/JBAAR.2019.141087
A. Mahmood, A. Al-kazaz, Khadier Z. Mayouf, A. Adhiah
Molecular expression (reverse transcription-quantitative polymerase chain reaction; RT-qPCR) and DNA-sequencing-based single nucleotide polymorphisms (SNPs) of interleukin 17A (IL17A) gene were determined in 51 etanercept-treated Iraqi rheumatoid arthritis (RA) patients and 45 control. The results revealed that the relative expression (2-∆∆Ct) of the IL17A gene was increased by 1.28 ± 0.29 fold in RA patients, and such profile was approximated in males (1.66 ± 0.58) and female (1.01 ± 0.28) patients. Concerning PCR-amplified DNA sequences, out of the 10 encountered SNPs, two SNPs (rs8193038 and rs3819025) showed allele frequencies that exceeded 10%. The rs8193038 SNP allele and genotype frequencies showed no significant variations between RA patients and control. The second SNP (rs3819025) was observed to have three genotypes (AA, AG, and GG). Among these genotypes, it was observed that the homozygous genotype of the mutant allele (GG) was only recorded in patients with a frequency of 13.7%, while none of the control had this genotype. Such a difference was significant even after the correction of probability (pc = 0.05), and the associated OR was 15.34 (95% C.I.: 1.39 - 169.24). It was also observed that G allele showed a significant increased frequency in patients (25.5 vs. 12.2%; OR = 2.46; 95% C.I.: 1.14 - 5.30; p = 0.015), while A allele frequency was significantly decreased (74.5 vs. 87.8%; OR = 0.41; 95% C.I.: 0.19 - 0.88; p = 0.015). However, the significance in both cases was lost when the probability was corrected. It was also observed that there was no significant impact of the rs3819025 SNP genotypes on the expression of the IL17A gene. In conclusion, the IL17A gene showed an increased expression in RA patients, and rs3024419 SNP is suggested to be associated with an increased risk to develop the disease in the Iraqi population.
{"title":"Molecular expression and single nucleotide polymorphisms of the IL17A gene among etanercept-treated rheumatoid arthritis patients","authors":"A. Mahmood, A. Al-kazaz, Khadier Z. Mayouf, A. Adhiah","doi":"10.21608/JBAAR.2019.141087","DOIUrl":"https://doi.org/10.21608/JBAAR.2019.141087","url":null,"abstract":"Molecular expression (reverse transcription-quantitative polymerase chain reaction; RT-qPCR) and DNA-sequencing-based single nucleotide polymorphisms (SNPs) of interleukin 17A (IL17A) gene were determined in 51 etanercept-treated Iraqi rheumatoid arthritis (RA) patients and 45 control. The results revealed that the relative expression (2-∆∆Ct) of the IL17A gene was increased by 1.28 ± 0.29 fold in RA patients, and such profile was approximated in males (1.66 ± 0.58) and female (1.01 ± 0.28) patients. Concerning PCR-amplified DNA sequences, out of the 10 encountered SNPs, two SNPs (rs8193038 and rs3819025) showed allele frequencies that exceeded 10%. The rs8193038 SNP allele and genotype frequencies showed no significant variations between RA patients and control. The second SNP (rs3819025) was observed to have three genotypes (AA, AG, and GG). Among these genotypes, it was observed that the homozygous genotype of the mutant allele (GG) was only recorded in patients with a frequency of 13.7%, while none of the control had this genotype. Such a difference was significant even after the correction of probability (pc = 0.05), and the associated OR was 15.34 (95% C.I.: 1.39 - 169.24). It was also observed that G allele showed a significant increased frequency in patients (25.5 vs. 12.2%; OR = 2.46; 95% C.I.: 1.14 - 5.30; p = 0.015), while A allele frequency was significantly decreased (74.5 vs. 87.8%; OR = 0.41; 95% C.I.: 0.19 - 0.88; p = 0.015). However, the significance in both cases was lost when the probability was corrected. It was also observed that there was no significant impact of the rs3819025 SNP genotypes on the expression of the IL17A gene. In conclusion, the IL17A gene showed an increased expression in RA patients, and rs3024419 SNP is suggested to be associated with an increased risk to develop the disease in the Iraqi population.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81341559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-21DOI: 10.21608/JBAAR.2019.138652
Basma M. Sheta, M. El-Alfy, H. El-Hamid
Studying migratory birds wintering stopover areas has a global concern. The contamination of heavy metals is one of the serious threats to birds in the wetland ecosystem. In the present study, heavy metal exposure risk to birds in Burullus Lake was assessed. The concentrations of Cr, Ni, Pb, Cd, and Co in water, plant, and fish were determined in 10 bird habitats. A heavy metals' exposure risk assessment model to migratory birds in Burullus Lake had been done. It showed that small waders, represented by dunlin, are exposed to higher doses and risk than the waterfowls, represented by mallard. Evaluation of this risk to migratory birds could not be done using water exposure doses only, but food exposure including plant and fish must be included. It is a priority to perform more efforts to reduce serious contamination with Co and Ni. The risk for fish feeders can be summarized according to the following order: Co > Ni > Cd > Pb > Cr, while the risk for plant feeders is: Co > Ni > Cr > Cd > Pb. The health risk of heavy metal exposures was calculated using Target Hazard Quotient (THQ) and Hazard index (HI). Results showed that THQs for all metals were below the standard (<1), while HI values were lower than one except for Co and Cd. HI for these two metals are considered an appreciable hazard risk to human health. Therefore, it is recommended to analyze the heavy metal risk on a temporal basis.
研究候鸟的越冬停留地是全球关注的问题。重金属污染是湿地生态系统中鸟类生存的严重威胁之一。本研究对布鲁勒斯湖鸟类重金属暴露风险进行了评价。测定了10个鸟类栖息地水、植物和鱼类中Cr、Ni、Pb、Cd和Co的浓度。建立了布鲁勒斯湖候鸟重金属暴露风险评价模型。研究表明，以鸭为代表的小涉禽比以绿头鸭为代表的水禽暴露于更高的剂量和风险。对候鸟的这种风险的评估不能只使用水接触剂量，但必须包括包括植物和鱼类在内的食物接触。当务之急是采取更多措施减少Co和Ni的严重污染。鱼类摄食者的风险大小为Co > Ni > Cd > Pb > Cr，植物摄食者的风险大小为Co > Ni > Cr > Cd > Pb。采用目标危害商(THQ)和危害指数(HI)计算重金属暴露的健康风险。结果表明，除Co和Cd外，所有金属的thq均低于标准(<1)，而HI值低于1，这两种金属的HI值被认为对人体健康有明显的危害风险。因此，建议在时间基础上分析重金属风险。
{"title":"A Heavy Metal Exposure Risk assessment Model to Migratory Birds and Human in Burullus Lake, Egypt","authors":"Basma M. Sheta, M. El-Alfy, H. El-Hamid","doi":"10.21608/JBAAR.2019.138652","DOIUrl":"https://doi.org/10.21608/JBAAR.2019.138652","url":null,"abstract":"Studying migratory birds wintering stopover areas has a global concern. The contamination of heavy metals is one of the serious threats to birds in the wetland ecosystem. In the present study, heavy metal exposure risk to birds in Burullus Lake was assessed. The concentrations of Cr, Ni, Pb, Cd, and Co in water, plant, and fish were determined in 10 bird habitats. A heavy metals' exposure risk assessment model to migratory birds in Burullus Lake had been done. It showed that small waders, represented by dunlin, are exposed to higher doses and risk than the waterfowls, represented by mallard. Evaluation of this risk to migratory birds could not be done using water exposure doses only, but food exposure including plant and fish must be included. It is a priority to perform more efforts to reduce serious contamination with Co and Ni. The risk for fish feeders can be summarized according to the following order: Co > Ni > Cd > Pb > Cr, while the risk for plant feeders is: Co > Ni > Cr > Cd > Pb. The health risk of heavy metal exposures was calculated using Target Hazard Quotient (THQ) and Hazard index (HI). Results showed that THQs for all metals were below the standard (<1), while HI values were lower than one except for Co and Cd. HI for these two metals are considered an appreciable hazard risk to human health. Therefore, it is recommended to analyze the heavy metal risk on a temporal basis.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89750517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-03-29DOI: 10.21608/jbaar.2019.138335
Kelly S. Levano, Luis Jaramillo-Valverde, Elizabeth Delgado, H. Guio
DNA isolation is the first step for most molecular tests, thus indicating the importance of using a reliable and suitable method for this procedure. The use of magnetic nanoparticles for blood DNA isolation offers several advantages including subjecting little mechanical stress to your sample, obtaining higher quality and quantity of DNA, and non-laborious procedures. In this study, we isolated genomic DNA using gelatin-coated magnetic nanoparticles from blood samples and test its use compare with three commercial blood DNA isolation kits. DNA purity and yield were assessed by measuring absorbance at A260/A280 and by agarose gel electrophoresis. The suitability of the isolated DNA for downstream applications was analyzed by endpoint PCR and Sanger sequencing. In this study, gelatin-coated magnetite particles for genomic DNA isolation provide an efficient, simple, and inexpensive method that does not require the use of commercial blood DNA Isolation Kit.
{"title":"Gelatin-coated magnetic nanoparticles-based DNA isolation method: A comparison with commercial DNA isolation kits from whole blood","authors":"Kelly S. Levano, Luis Jaramillo-Valverde, Elizabeth Delgado, H. Guio","doi":"10.21608/jbaar.2019.138335","DOIUrl":"https://doi.org/10.21608/jbaar.2019.138335","url":null,"abstract":"DNA isolation is the first step for most molecular tests, thus indicating the importance of using a reliable and suitable method for this procedure. The use of magnetic nanoparticles for blood DNA isolation offers several advantages including subjecting little mechanical stress to your sample, obtaining higher quality and quantity of DNA, and non-laborious procedures. In this study, we isolated genomic DNA using gelatin-coated magnetic nanoparticles from blood samples and test its use compare with three commercial blood DNA isolation kits. DNA purity and yield were assessed by measuring absorbance at A260/A280 and by agarose gel electrophoresis. The suitability of the isolated DNA for downstream applications was analyzed by endpoint PCR and Sanger sequencing. In this study, gelatin-coated magnetite particles for genomic DNA isolation provide an efficient, simple, and inexpensive method that does not require the use of commercial blood DNA Isolation Kit.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89980880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-03-28DOI: 10.21608/jbaar.2019.138334
W. Fathy, Sabah Farouk, Gamalat A Elgedawy, A. Abd-Elghany,, Amira Ahmed
Waleed M Fathy, Sabah Farouk, Gamalat Elgedawy, Ayman Abd-Elhakim Abd-Elghany, Amira Ahmed Clinical Pathology Department, Faculty of Medicine, Menufia University, Egypt Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, Sadat City University Egypt Biochemistry Department, National Liver Institute, Menufia University, Egypt Obstetrics and Gynecology Department, General Organization of Teaching Hospital, Egypt.
Waleed M Fathy, Sabah Farouk, Gamalat Elgedawy, Ayman Abd-Elhakim Abd-Elghany, Amira Ahmed临床病理学系，埃及Menufia大学医学院分子生物系，基因工程与生物技术研究所，萨达特城市大学埃及生物化学系，Menufia大学国家肝脏研究所，埃及妇产科，埃及教学医院总组织。
{"title":"Assessment of cell-free DNA for early detection of ovarian cancer in women","authors":"W. Fathy, Sabah Farouk, Gamalat A Elgedawy, A. Abd-Elghany,, Amira Ahmed","doi":"10.21608/jbaar.2019.138334","DOIUrl":"https://doi.org/10.21608/jbaar.2019.138334","url":null,"abstract":"Waleed M Fathy, Sabah Farouk, Gamalat Elgedawy, Ayman Abd-Elhakim Abd-Elghany, Amira Ahmed Clinical Pathology Department, Faculty of Medicine, Menufia University, Egypt Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, Sadat City University Egypt Biochemistry Department, National Liver Institute, Menufia University, Egypt Obstetrics and Gynecology Department, General Organization of Teaching Hospital, Egypt.","PeriodicalId":15163,"journal":{"name":"Journal of Bioscience and Applied Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2019-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90435413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-03-26DOI: 10.21608/jbaar.2019.121250
El-Hassan M. Mokhamer, E. Radwan, M. Elsaka
The main purpose of this study is to evaluate the impact of heavy metals (Pb, Zn, Cu, Fe, and Cd) aquatic pollution of EL-Mahmoudeyia canal on the antioxidant enzymatic activities, GSH content and lipid peroxidation levels (MDA) in Oreochromis. niloticus muscles tissues collected from two areas ELMahmoudeyia canal as Polluted area and Rosetta branch of river Nile as reference area in summer 2018 and winter 2019 as well as DNA damage was assessed in fish gills(erythrocytes) samples by applying comet assay. EL-Mahmoudeyia canal exposed to excessive of industrial effluents which impact the living organisms especially fish. The herein results showed that higher concentrations of heavy metals (Pb, Zn, Cu, Fe, and Cd) were detected in water and fish samples collected from the polluted area in comparison with the reference area, especially in winter. The accumulation patterns of heavy metals in muscles of O. niloticus, were in the following order: Fe > Zn >Pb> Cu and Cd.The antioxidant enzymatic activities of (SOD, CAT, GPx and GST) and the lipid peroxidation biomarker MDA levels in muscles of O. niloticus collected from the polluted area were found to be significantly increased compared to that of the reference area. Meanwhile, there was a significant decrease in the GSH content level in muscles of O. niloticus collected from the polluted area compared to that of the reference area. A significant elevation in DNA damage frequencies was observed in fish collected from the polluted areas compared with those from the reference area. These noticeable alterations in the selected antioxidant enzymatic activities in muscles of the O. niloticus go in parallel with the remarkable elevation in the levels of the detected heavy metals in water from EL Mahmoudeyia canal, as a result of pollution in these areas. This study explored the utility of the DNA damage, the altered antioxidant enzymatic activities, GSH content and MDA level as biomarkers of aquatic pollution.
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