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Identification of bacteria species among Enterobacteriaceae found in confectionery cakes 糕点中发现的肠杆菌科细菌的菌种鉴定
Pub Date : 2023-12-29 DOI: 10.31895/hcptbn.18.3-4.2
Jasenka Petrić, B. Hengl, I. Kovaček, Ksenija Markov, Đ. Ačkar, D. Knežević
The goal of this research was to identify which Enterobacteriaceae species are present in cakes from confectionery establishments and whether these are species that can be considered pathogenic for humans and what their harmful impact on human health could be. The sampling of confectionery cakes was carried out in the area of the City of Zagreb, and the samples were analysed for the presence of Enterobacteriaceae in accordance with the HRN ISO 21528-2:2017 standard. In cases where the samples contained the number of Enterobacteriaceae greater than 102 CFU/g, the MALDI-TOF method was used to identify individual species of enterobacteria. The results of the analyses determined the presence of 10 Enterobacteriaceae species: Enterobacter kobei, Enterobacter cloacae, Pantoea agglomerans, Serratia liquefaciens, Enterobacter asburiae, Klebsiella oxytoca, Buttiauxella gaviniae, Buttiauxella warmboldiae, Raoultella and Cedecea neteri. All species of Enterobacteriaceae determined in this research, according to the literature, were previously isolated from food or water, and all species except Buttiauxella warmboldiae were isolated from humans and the environment. However, for none of the species of Enterobacteriaceae determined in this research a literature reference to support food as a vehicle in case of human illness was found.
这项研究的目的是确定糖果店的蛋糕中含有哪些肠杆菌科细菌,这些细菌是否可被视为对人类具有致病性,以及它们对人类健康可能产生的有害影响。在萨格勒布市地区对糕点进行了取样,并根据 HRN ISO 21528-2:2017 标准对样品中是否含有肠杆菌科细菌进行了分析。如果样本中的肠杆菌数量超过 102 CFU/g,则采用 MALDI-TOF 方法来鉴定肠杆菌的各个种类。分析结果确定了 10 种肠杆菌:这 10 种肠杆菌科细菌是:Enterobacter kobei、Enterobacter cloacae、Pantoea agglomerans、Serratia liquefaciens、Enterobacter asburiae、Klebsiella oxytoca、Buttiauxella gaviniae、Buttiauxella warmboldiae、Raoultella 和 Cedecea neteri。根据文献资料,本研究中确定的所有肠杆菌科菌种以前都是从食物或水中分离出来的,除了温鲍氏布氏菌外,其他菌种都是从人类和环境中分离出来的。不过,在本次研究中确定的肠杆菌科细菌中,没有发现任何一种细菌的文献资料支持将食物作为人类疾病的载体。
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引用次数: 0
Development, bioactive and sensory analysis of the honey-filled chocolate pralines infused with ground ivy (Glechoma hederacea L.) extract 常春藤(Glechoma hederacea L.)提取物浸泡蜂蜜巧克力果仁糖的开发、生物活性和感官分析
Pub Date : 2023-12-29 DOI: 10.31895/hcptbn.18.1-2.6
Danijela Šeremet, Petra Subotić, K. Marković, Ana Mandura Jarić, Aleksandra Vojvodić Cebin, Nada Vahčić, D. Komes
Chocolate is consumed largely worldwide and it is known as one of the most craved foods. The market of chocolate products is growing steadily and is expected to continue to grow in the coming years. The aim of the present study was to formulate chocolate pralines with enriched sensory and bioactive attributes by incorporating different types of honey (false indigo, buckwheat, rapeseed, mandarin and sage) and ground ivy (Glechoma hederacea L.) extract as ingredients of the filler. The honey samples were subjected to physico-chemical analysis, sugar analysis using the HPLC-RID methodology, as well as to evaluation of antioxidant capacity. The bioactive and sensory properties and sugar composition of the formulated pralines were determined. Bioactive characterization included the determination of phenolic profile by HPLC-PAD methodology. Buckwheat honey showed the most pronounced antioxidant capacity with the value of 3.21 and 2.06 µmol Trolox/g dmb, determined by ABTS and DPPH assays, respectively, while the lowest values were measured for mandarin honey (0.41 and 0.32 µmol Trolox/g dmb). Specific bioactive compounds of cocoa: epicatechin, theobromine and caffeine, were detected in all formulated chocolate pralines, as well as the most predominant phenolic compounds of ground ivy: chlorogenic, caffeic, rosmarinic acid and rutin. Such enriched bioactive composition contributed to the pronounced antioxidant capacity of chocolate pralines. Finally, chocolate pralines prepared with mandarin honey were sensory evaluated as the sweetest and gained the highest scores for the overall acceptability.
巧克力在全世界的消费量很大,被称为最受欢迎的食品之一。巧克力产品市场正在稳步增长,预计未来几年还将继续增长。本研究的目的是通过添加不同类型的蜂蜜(假靛蓝、荞麦、油菜籽、柑橘和鼠尾草)和常春藤(Glechoma hederacea L.)提取物作为填充物成分,配制出具有丰富感官和生物活性属性的巧克力果仁糖。对蜂蜜样品进行了理化分析,采用 HPLC-RID 方法进行了糖分分析,并评估了抗氧化能力。测定了配制果仁糖的生物活性、感官特性和糖成分。生物活性特性包括采用 HPLC-PAD 方法测定酚类物质的概况。通过 ABTS 和 DPPH 法测定,荞麦蜂蜜的抗氧化能力最强,分别为 3.21 和 2.06 µmol Trolox/g dmb,而柑橘蜂蜜的抗氧化能力最低(0.41 和 0.32 µmol Trolox/g dmb)。在所有配制的果仁巧克力中都检测到了可可的特定生物活性化合物:表儿茶素、可可碱和咖啡因,以及常春藤中最主要的酚类化合物:绿原酸、咖啡酸、迷迭香酸和芦丁。这种丰富的生物活性成分有助于提高巧克力果仁糖的抗氧化能力。最后,用柑橘蜜制作的巧克力果仁糖在感官评价中是最甜的,在总体可接受性方面得分最高。
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引用次数: 0
Imunoenzimske metode u procjeni kvalitete meda i pčelinjih proizvoda
Pub Date : 2021-12-31 DOI: 10.31895/hcptbn.16.3-4.2
Karla Rotim, Ksenija Marković, Nada Vahčić
Imunoenzimska ELISA metoda (engl. Enzyme-Linked ImmunoSorbent Assay) se istražuje u posljednje vrijeme kao brza, jednostavna i visokospecifična analitička metoda za unaprjeđenje i standardizaciju kvalitete meda i pčelinjih proizvoda. Med i pčelinji proizvodi se osim zbog nutritivne vrijednosti učestalo koriste i zbog brojnih pozitivnih bioloških učinaka, a ELISA metoda svoju primjenu u standardizaciji njihove kvalitete može pronaći kod utvrđivanja autentičnosti, botaničkog podrijetla te razlikovanja uzoraka prirodnog meda. Metoda se razvija u svrhu određivanja pčelinjih proteina i peptida, kao što su apalbumin-1 i defensin-1, a koji mogu predstavljati biljege izvornosti. Imunoenzimska ELISA metoda primjenu pronalazi i u određivanju peludi određene biljne vrste u svrhu utvrđivanja botaničkog podrijetla, te pri procjeni kvalitete određivanjem enzima, kao i u svrhu razlikovanja uzoraka prirodnog meda određivanjem specifičnih proteina.
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引用次数: 0
High Hydrostatic pressure 高静水压力
Pub Date : 2021-12-31 DOI: 10.31895/hcptbn.16.3-4.3
Marko Škegro, S. Karlović, D. Ježek, T. Bosiljkov, M. Brnčić, Marko Marelja, F. Dujmić
Proces obrade visokim hidrostatskim tlakom, kao netoplinski proces, se koristi za inaktivaciju mikroorganizama uz minimalnu preinaku same namirnice. Postiže se jednaki standard sigurnosti hrane kao kod toplinske pasterizacije te se zadovoljavaju zahtjevi potrošača za svježim i minimalno procesiranim proizvodima uz kraće vrijeme obrade za razliku od konvencionalnih tehnika. U novijim istraživanjima, visoki tlak se smatra zaslužnim za promjene u strukturi stanice i biopolimera u stanicama obrađivanih namirnica što rezultira boljem vezivanju vode, procesima želiranja te nastanku novih tekstura i proizvoda. Ovaj rad prezentira upotrebu visokog hidrostatskog tlaka kod procesiranja crvenog mesa i mesnih prerađevina, voća i povrća te utjecaj na mikroorganizme u namirnici. Uz pasterizaciju, proces se koristi i za ekstrakciju bioaktivnih tvari iz sirovina, uz značajno bolje prinose i kvalitetu u usporedbi s konvencionalnim metodama ekstrakcije.
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引用次数: 26
Vegetable by-products in the European food legislative framework 欧洲食品立法框架中的蔬菜副产品
Pub Date : 2021-12-31 DOI: 10.31895/hcptbn.16.3-4.1
N. Knežević, Jasmina Ranilović, Tanja Cvetković, Slavka Grbavac, Marina Palfi
Vegetable by-products are a source of many useful bioactive compounds, dietary fiber, fatty acids and proteins that have a great potential in the development of new food products and the promotion of the circular economy and sustainability.Considering processed pepper by-products as an example, the aim of this paper is to provide an overview of EU food legislation that should be taken into account before placing such products on the market, as well as of the voluntary labeling of functional, nutritional and sustainability benefits important for consumers.
蔬菜副产品是许多有用的生物活性化合物、膳食纤维、脂肪酸和蛋白质的来源,在开发新的食品和促进循环经济和可持续发展方面具有很大的潜力。以加工辣椒副产品为例,本文的目的是概述在将此类产品投放市场之前应考虑的欧盟食品立法,以及对消费者重要的功能,营养和可持续性利益的自愿标签。
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引用次数: 1
Jestivo cvijeće
Pub Date : 2021-12-31 DOI: 10.31895/hcptbn.16.3-4.4
Marija Badanjak Sabolović, Lucija Jurašinović, Suzana Rimac Brnčić
Jestivo cvijeće dio je tradicionalne medicine već stoljećima, ali se koristi i za pripremu različitih jela poput juha i salata te kao dekoracija. U zadnje vrijeme, jestivo cvijeće postaje kulinarski trend. Sve veći interes za jestivo cvijeće u kulinarstvu potiču kuharice, kulinarski članci te televizijske emisije s naglaskom na njihove hranjive i funkcionalne sastojke. Cilj ovog rada je dati pregled dosadašnjih spoznaja o najzastupljenijim vrstamajestivog cvijeća na tržištu vezano uz njihovu nutritivnu vrijednost, antioksidacijsku aktivnost, funkcionalnost, a obuhvaća cvjetove ukrasnog i začinskog bilja.
酢浆草是许多食客的传统药材,除了煲汤、做沙拉外,还可以用来制作各种菜肴,也可以作为装饰品。近年来,食用蔬菜已成为一种烹饪趋势。厨师、烹饪文章和电视节目都在强调蔬菜的营养和功能特性,烹饪界对食用蔬菜的兴趣也与日俱增。本建议旨在概述目前市场上最常见的活体蔬菜的营养价值、抗氧化活性、功能性以及根茎和辛辣香草的味道。
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引用次数: 0
Overview of packaging materials for Dairy packaging 乳品包装材料概述
Pub Date : 2021-12-31 DOI: 10.31895/hcptbn.16.3-4.5
Irena Barukčić, M. Ščetar, Katarina Lisak Jakopović, M. Kurek, R. Božanić, K. Galić
Regarding milk producing, it is well known that processing and packaging are the two equally important operational phases. Packaging is the last, but definetly not the less important phase. Various automation strategies are constantly being utilized in every phase of processing, packaging and production/use of new packaging materials.The most suitable packaging will provide adequate properties to pack liquid milk and dairy products which have an important role to prolonged product shelf life. Temperature and moisture are important parameters which affect the shelf life of packaged milk and dairy products, due to their effect on the bacterial growth especially in fermented milk products. The aim of this review article is to introduce advantages and disadvantages and principles of packaging methods and materials correlated to shelflife of dairy products.
关于牛奶生产,众所周知,加工和包装是两个同等重要的操作阶段。包装是最后一个,但绝对不是次要的阶段。在加工、包装和生产/使用新包装材料的各个阶段,不断采用各种自动化策略。最合适的包装将提供足够的性能来包装液体牛奶和乳制品,这对延长产品的保质期有重要作用。温度和湿度是影响包装牛奶和乳制品保质期的重要参数,因为它们对细菌生长有影响,特别是在发酵乳制品中。本文旨在介绍与乳制品货架期相关的包装方法和材料的优缺点和原理。
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引用次数: 0
Biological denitrification 生物脱氮
Pub Date : 2021-06-01 DOI: 10.31895/hcptbn.16.1-2.4
Dijana Grgas, T. Landeka Dragičević, A. Štrkalj, Andrijana Brozinčević, Mirjana Galant, Tea Štefanac
Ubrzani napredak industrije, poljoprivrede i domaćinstva su pogodovali povišenim koncentracijama dušika u vodenom ekosustavu, što uzrokujeeutrofikaciju. Dušik se iz otpadne vode uklanja procesom biološke denitrifikacije. U ovom preglednom radu dan je osvrt na denitrifikaciju, s aspektamikroorganizama, koncentracije otopljenog kisika, donora i akceptora elektrona.
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引用次数: 68
Breastfeeding: Health benefits and dietary recommendations 母乳喂养:健康益处和饮食建议
Pub Date : 2021-06-01 DOI: 10.31895/hcptbn.16.1-2.1
Ines Panjkota Krbavčić, Anja Vukomanović
Breastfeeding is a natural way of feeding a newborn as it provides all the essential nutrients necessary for appropriate growth and development. Also, it is a delicate period when the emotional and physical connection between mother and child is created, providing the child with a sense ofsecurity, relaxation, and tenderness. Under that, the devotion of institutions and various associations to raise social and health awareness and promote the importance of breastfeeding is constantly increasing. World Health Organization (WHO) recommends exclusive breastfeeding during the first six months of an infant’s life, which provides the child many short- and long-term benefits. When talking about benefits, the emphasis is put primarily on the wellbeing of the child, while the focus on the needs of a nursing mother should be of equal importance. The period of lactation and breastfeeding demands the mother’s greater energy intake than the period of the last three-semester of pregnancy to meet all micro- and macronutrient needs of mother and child. The volume and the composition of milk, respectively the quality of infant’s nutrition, partially depends on the quality of the mother’s nutrition, and partially on the mother’s physical supplies from which nutrients are being compensated in the case of a dietary deficit. To avoid the negative effects of lactation and breastfeeding on the nutritional and health status of the mother in upcoming periods of life, it is crucial to give special attention to various, balanced, and energy-satisfying nutrition. Although the primary goal is to achieve the recommended intake of all essential nutrients by food intake, in cases of unbalanced nutrition, health limitations, or practicing restrictive diets, the use of dietary supplementation is needed. Because of the biological needs and fortified deficits, in dietary supplements for pregnant women could often be found iron, calcium, zinc, iodine, folate, and vitamins B12, C and D.
母乳喂养是一种自然的喂养新生儿的方式,因为它提供了适当生长和发育所需的所有必需营养素。同时,这也是一个微妙的时期,母亲和孩子之间的情感和身体联系被创造出来,为孩子提供了一种安全感、放松感和温柔感。在这种情况下,各机构和各种协会不断致力于提高社会和健康意识,宣传母乳喂养的重要性。世界卫生组织(世卫组织)建议在婴儿出生后的头六个月进行纯母乳喂养,这为儿童提供了许多短期和长期的好处。在谈到福利时,重点主要放在儿童的福祉上,而关注哺乳母亲的需求也应该同等重要。在哺乳期和哺乳期,母亲需要比怀孕最后三个学期更多的能量摄入,以满足母亲和孩子所有微量和宏量营养素的需求。乳汁的量和成分,分别是婴儿的营养质量,部分取决于母亲的营养质量,部分取决于母亲在饮食不足的情况下通过身体供应来补充营养。为了避免哺乳和母乳喂养对母亲在即将到来的生命时期的营养和健康状况的负面影响,特别注意各种、平衡和能量满足的营养是至关重要的。虽然主要目标是通过食物摄入达到所有必需营养素的推荐摄入量,但在营养不均衡、健康受限或实行限制性饮食的情况下,需要使用膳食补充剂。由于生理需求和强化缺陷,孕妇的膳食补充剂中通常含有铁、钙、锌、碘、叶酸以及维生素B12、C和D。
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引用次数: 1
Aerobni granulirani mulj u obradi otpadnih voda
Pub Date : 2021-06-01 DOI: 10.31895/hcptbn.16.1-2.3
Dijana Grgas, Tibela Landeka Dragičević, Anita Štrkalj, Andrijana Brozinčević, Adelina Ladavac, Tea Štefanac, Mirjana Galant
Aerobni granulirani mulj (AGS, engl. Aerobic Granular Sludge) predstavlja obećavajuću tehnologiju u obradi otpadnih voda kućanstva i industrije.Aerobne granule su samoimobilizirane mikrobne nakupine, bez nosača, a karakterizira ih kompaktna gusta struktura, visoko zadržavanje biomase,visoka učinkovitost uklanjanja onečišćenja i svojstvo brzog taloženja. Na formiranje i stabilnost aerobnog granuliranog mulja utječu brojničimbenici, poput koncentracija otopljenog kisika, vrijeme prozračivanja, vrsta izvora ugljika, sile smicanja, period gladovanja, vrijeme taloženja.Zbog slojevite strukture granule, sa vanjskim aerobnim slojem i anoksičnim i anaerobnim zonama prema središtu granule, moguće je istovremenouklanjanje C, N i P. AGS tehnologija ima potencijal smanjenja infrastrukture i operativnih troškova pročišćavanja otpadnih voda. Ovaj rad dajepregled najnovijih spoznaja iz literature o mehanizmu granulacije i svojstvima aerobnih granula.
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引用次数: 0
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Hrvatski časopis za prehrambenu tehnologiju, biotehnologiju i nutricionizam
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