Journal of Cellular Physiology最新文献

RETRACTION: D. Pal, S. Sur, R. Roy, S. Mandal, and C. Kumar Panda, “Epigallocatechin Gallate in Combination With Eugenol or Amarogentin Shows Synergistic Chemotherapeutic Potential in Cervical Cancer Cell Line,” Journal of Cellular Physiology 234, no. 1 (2019): 825–836, https://doi.org/10.1002/jcp.26900.

The above article, published online on 4 August 2018 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the journal Editor-in-Chief, Robert Heath; and Wiley Periodicals LLC. The retraction has been agreed due to concerns raised by third parties. Figures 3A and 7B were found to contain duplicated image elements that were presented in different scientific contexts. Due to the time elapsed since publication, the authors were unable to retrieve the raw data underlying the article, and the clarification they provided was insufficient to address the concerns. Accordingly, the article has been retracted, as the editors have lost confidence in the accuracy and the integrity of the whole body of data presented. The authors have been informed of the retraction decision and disagree with it.

Calpain-1 and -2 are calcium-dependent cysteine proteases associated with sperm processes, such as capacitation, the acrosomal reaction, and motility, making them important for the acquisition of fertilizing capacity among spermatozoa. Moreover, their inhibition significantly reduces in vitro fertilization. Guinea pig spermatozoa express only calpain-1, which has been implicated in spectrin cytoskeletal remodeling and NOX2 and NOX4 activation during capacitation. However, little is known about the mechanisms by which calpains participate in capacitation and the acrosomal reaction. The interaction of spectrin with phospholipids maintains the asymmetry of the plasma membrane. Since spectrin is a significant target of calpain, calpain may be involved in the dynamics of phospholipids and other membrane lipids during the capacitation process. Therefore, this work aims to elucidate the role of calpain in capacitation and acrosomal reactions, as well as its relationship with the dynamics of different membrane lipids related to capacitation and the acrosomal reaction. The results show that calpain-1 inhibition by calpeptin significantly reduced the number of spermatozoa that underwent capacitation and acrosomal reactions. Inhibition of calpain-1 also blocked protein phosphorylation at Tyr, as well as calcium influx and actin polymerization, which required for successful capacitation. Calpain inhibition also prevented phosphatidylserine translocation and the dynamics of caveolin-1, both processes associated with capacitation and the acrosome reaction. Sperm capacitation in the presence of cholesterol prevented the dynamics of phosphatidylserine, GM1 and caveolin-1. However, calpain-1 inhibition did not prevent cholesterol or GM1 ganglioside dynamics. The results of this investigation provide strong evidence for the mechanisms by which calpain-1 regulates capacitation and the acrosome reaction in guinea pig spermatozoa, suggesting that calpain-1 is a key player in optimal capacitation and the acrosome reaction.

Palmitic acid (PA), the most abundant saturated fatty acid (SFA) in humans, plays a key role in energy metabolism, membrane synthesis, and signaling. Oligodendrocyte precursor cells (OPCs), which generate mature oligodendrocytes (OLs) forming the myelin sheath, are responsive to metabolic and redox signals. Despite increasing interest in lipid metabolism and mitochondrial dynamics as regulators of OPC fate, the effects of PA remain unclear. This study investigates the biphasic, dose-dependent effects of PA on OPCs using the oligodendrocyte precursor MO3.13 cell line and employs rat organotypic slice cultures to evaluate the effects of non-toxic PA doses under pathological conditions and on axonal (re)-myelination. In MO3.13 cells, high-dose PA (100 µM) induces mitochondrial fragmentation and caspase-7 activation, accompanied by reduced mitofusin-2 (MFN2) and phosphorylated dynamin-related protein 1 at Ser616 (p-DRP1), indicating altered fusion-fission balance and impaired reactive oxygen species (ROS) generation. In contrast, low-dose PA (25 µM) triggers a protective response involving nuclear factor erythroid 2-related factor 2 (Nrf2) activation and upregulation of antioxidant and lipid-regulatory genes (glutamate-cysteine ligase modifier subunit [GCLM], NAD(P)H dehydrogenase [quinone] 1 [NQO1], peroxisome proliferator-activated receptor gamma [PPARγ], and cluster of differentiation 36 [CD36]) resulting in reduced intracellular ROS and enhanced lipid mobilization. PA 25 µM promotes OPC differentiation by inhibiting migration and cell cycle progression and increasing myelin basic protein (MBP) and proteolipid protein (PLP) expression. Notably, early exposure (1 day) favors mitochondrial fusion, whereas prolonged exposure (4 days) shows a physiological shift to fission. PA 25 µM prevents neurodegeneration in hippocampal organotypic slice cultures exposed to a neuroinflammatory insult. In cerebellar organotypic slice cultures, PA 25 µM enhances axonal myelination and accelerates remyelination following lysolecithin-induced demyelination. These findings highlight the physiological relevance of low-dose PA in modulating OLs.

Retraction: H. Li, J. Yang, X. Wei, C. Song, D. Dong, Y. Huang, X. Lan, M. Plath, C. Lei, Y. Ma, X. Qi, Y. Bai, and H. Chen, "CircFUT10 Reduces Proliferation and Facilitates Differentiation of Myoblasts by Sponging miR-133a," Journal of Cellular Physiology 233, no. 6 (2018): 4643-4651, https://doi.org/10.1002/jcp.26230. The above article, published online on 18 October 2017 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the journal Editor-in-Chief, Robert Heath; and Wiley Periodicals LLC. The retraction has been agreed upon following concerns raised by a third party regarding data presented in the article. An investigation identified duplication between the two MyoD bands shown in Figure 3B and two MyhC bands in Figure 3H. All MyhC bands in Figure 3H were also found to be identical to western blot bands published in another article by the same authors, where they were presented as representing a different protein. Furthermore, overlaps were identified between images in Figure 6 A and images published in another article by the same authors, which were also used to represent different material. Finally, the miR-133a flow cytometry data shown in Figure 6B was also found to be identical to a dot plot found in another article published by the same authors, again representing different material. The authors cooperated with the investigation and provided some supporting data; however, this was not sufficient to restore the editors' confidence in the results. The editors consider the results and conclusions unreliable. The authors did not respond when asked to agree to the final wording of the retraction.